ライフサイエンスコーパス: bleb

1 actor 2 (MLF2) as a luminal component of the bleb.

2 ents but to different degrees in the growing bleb.

3 lated with the size of the intradermal fluid bleb.

4 sion to account for the shape of a traveling bleb.

5 es of 13 consecutive patients with a leaking bleb.

6 ed safely in eyes with functioning filtering blebs.

7 fficient to both induce and suppress nuclear blebs.

8 e pseudopods or, unexpectedly, migrate using blebs.

9 cilitate viral spread via infected apoptotic blebs.

10 ccurs, and naturally gives rise to traveling blebs.

11 s concentrated kinase activity within leader blebs.

12 pots represent three-dimensional unilamellar blebs.

13 antity of astrocyte-derived round membranous blebs.

14 ial association of PIP(2) and Kras(V12) with blebs.

15 zed proteins, but only PIP(2) is enhanced on blebs.

16 ropel themselves, including by pseudopods or blebbing.

17 e RhoC signaling during cell contraction and blebbing.

18 palatal shelf development, and subepidermal blebbing.

19 rtex, which may pressurize the cytoplasm for blebbing.

20 ensive cell contraction followed by membrane blebbing.

21 ne protrusions that resemble plasma membrane blebbing.

22 c machinery, and extensive cortical membrane blebbing.

23 l death but morphologically prevent membrane blebbing.

24 ronectin and cadherin 2 function to suppress blebbing.

25 a(2+)-dependent swelling (-80%) and membrane blebbing (-90%); 3) reduced calpain-dependent protein cl

26 formation of cellular protrusions in form of blebs, a type of protrusion found in various cell types.

27 Membrane blebbing accompanies various cellular processes, includi

28 MICS) technique to repair late-onset leaking blebs after trabeculectomy.

29 defects characterized by the development of blebs along the hair shaft in mice.

30 Cell contraction and blebbing also frequently occur as part of the cytopathic

31 nsible for the deregulation of the filtering bleb and subsequent loss of IOP control.

32 ons, but the interplay between the expanding bleb and the membrane tubes remains poorly understood.

33 All patients showed flat filtering bleb and uncontrolled IOP (34.5 +/- 11 mmHg), under maxi

34 on of Krtap5-5 from cancer cells led to cell blebbing and a loss of keratins 14 and 18, in addition t

35 , we show that hyen D induces rapid membrane blebbing and cell necrosis.

36 sembled vesicles and, in some cases, promote blebbing and division.

37 um response factor) pathway for sustained PM blebbing and entotic invasion.

38 e actin cortex in cells during initiation of blebbing and in blebs at different stages of their expan

39 ally and structurally distinct from membrane blebbing and involves disruption to the platelet microtu

40 the evagination occurs via a process akin to blebbing and is not driven by actin polymerization.

41 rop in cell height potentiated by persistent blebbing and loss of cortical F-actin homogeneity.

42 tion state is a major determinant of nuclear blebbing and morphology via its contribution to nuclear

43 nuclear deformation that may lead to nuclear blebbing and nuclear envelope rupture.

44 cells in safeguarding neurons against axonal blebbing and poliomyelitis from murine betacoronavirus-i

45 Both the dynamics of blebbing and the connection to nuclear pore biogenesis r

46 automatically finds lamellipodia, filopodia, blebs and other motifs.

47 , Ezrb and cell-adhesion proteins to inhibit blebs and promote polarized actin-rich protrusive activi

48 Blebs and pseudopods can both power cell migration, with

49 or dDia2 restores cells' ability to initiate blebs and thus migrate, though pseudopods are still lost

50 rosis and hypotony, a filtering conjunctival bleb, and focal scleromalacia with localized pigmentatio

51 ation, nanopore-conducted currents, membrane blebbing, and cell death.

52 ng, clathrin-mediated endocytosis, polarized blebbing, and endocytic vesicle sorting.

53 e in cellular Tropomyosin-3, plasma membrane blebbing, and release of 0.1- to 1-mum-diameter MPs.

54 is accompanied by nuclear volume expansion, blebbing, and rupture, ultimately resulting in reduced c

55 lanoma cells from actin-driven protrusion to blebbing, and we present tools to quantify how cells man

56 gonistically to organize actin within leader blebs, and Erk mediates this effect.

57 icial and deep cells: lamellipodia are lost, blebs appear instead, and collective migration fails.

58 dications, bleb morphology using the Indiana Bleb Appearance Grading Scale, and complication rates.

59 Nuclear blebs are associated with both lamin and chromatin alter

60 Blebs are extruded by intracellular pressure and are ini

61 mean aspect ratio h /d=0.38, indicating the blebs are mostly oblate.

62 Blebs are pressure-driven cell protrusions implicated in

63 Blebs are pressure-driven protrusions that play an impor

64 Membrane blebs are specialized cellular protrusions that play div

65 l nuclear morphology and protrusions termed "blebs" are diagnostic markers for many human afflictions

66 mean (SD) grades were 1.4 (0.1) for central bleb area, 1.4 (0.1) for bleb height, and 3.4 (0.2) for

67 Central bleb area, maximal bleb area, and bleb height were graded on a scale of 1 t

68 Central bleb area, maximal bleb area, and bleb height were grade

69 erwise, with a patent filtration and diffuse bleb as confirmed with anterior segment OCT imaging.

70 Using nuclear envelope (NE) blebbing as a phenotypic measure, we establish a direct

71 ly more tense, vascularized, or encapsulated blebs as a late complication (P = .046).

72 o membrane conformations and define membrane blebs as cellular compartments for direct interactions o

73 As previous studies have used blebs as reporters of membrane tension and pressure dyna

74 d in a 5-fold increase in surface-associated blebs, as well an onset of a wrinkled surface morphotype

75 ipodia, filopodia, invadopodia, and membrane blebs, as well as on cell-cell and cell-extracellular ma

76 crease in heterochromatin suppresses nuclear blebbing associated with nuclear rupture and DNA damage.

77 n-regulated MRTF transcriptional pathway for bleb-associated invasive motility, such as during entosi

78 related (eg, post-penetrating keratoplasty), bleb-associated, glaucoma drainage device-associated, an

79 loss of OM material through vesiculation and blebbing at cell-division sites and compensatory shrinka

80 Moreover, Ca(2+) can induce some blebbing at mM concentrations but not nearly as effectiv

81 n cells during initiation of blebbing and in blebs at different stages of their expansion-retraction

82 017) examine the membrane source that allows bleb-based cell migration in vivo.

83 and therefore suppresses efficient amoeboid bleb-based invasion.

84 Because amoeboid leader bleb-based migration (LBBM) occurs in confined spaces an

85 Eps8 to enhance cortex tension and drive the bleb-based migration of cancer cells under non-adhesive

86 e actin cortex mechanics and thereby mediate bleb-based migration of cancer cells.

87 ing these components, revealing a processive bleb-based protrusion program that is mechanistically di

88 to switch between mesenchymal and amoeboid (bleb-based) migration.

89 omes were not associated with the age of the bleb but were more likely in Black patients.

90 ependent of ubiquitin conjugation within the bleb, but strictly dependent on POM121, a transmembrane

91 easurements suggest that Zn(2+) caused lipid blebbing by decreasing the area per lipid on the side of

92 Blebbing can be divided into three distinct phases: bleb

93 ed myotube apoptosis (widespread microscopic blebs, caspase 3/7 activation, and annexin V binding at

94 Recent experiments involving blebbing cells have led to conflicting hypotheses regard

95 s, bleb morphology score using the Wuerzburg bleb classification score and any added glaucoma medicat

96 th only 20% of the silenced cells developing blebs compared with 53% of the control cells.

97 y this minimal invasive method, hypertrophic bleb complication of XEN gel implant has been successful

98 Nuclear envelope herniations (blebs) containing FG-nucleoporins and ubiquitin are the

99 Bleb contents are trapped by the narrow neck that separa

100 This inhibition of cellular blebbing correlated with a 25% decrease in cytosolic fre

101 determined by FRET analysis both at cell and bleb cortices.

102 njection site but within the confines of the bleb created by the intradermal bolus.

103 e particles was probed by TIRF microscopy on bleb-derived supported membranes.

104 only 1 of the 40 eyes did a cystic avascular bleb develop, with all the other eyes being non-cystic i

105 s early as postnatal Day 3 (P3); thereafter, blebs devoid of actin or tubulin appeared at the region

106 or mean injection volumes of 60 muL the mean bleb diameter is ~8 mm, with mean aspect ratio h /d=0.38

107 of migration from primarily pseudopodial to bleb driven in <30 s.

108 ugh the contractile actin cortex involved in bleb-driven motility is well characterized, a role for t

109 The transition to bleb-driven motility requires extracellular calcium and

110 e propose that mechanical resistance induces bleb-driven movement in Dictyostelium, which is chemotac

111 ontrols the ability of cells to contract and bleb during a variety of cellular processes, including c

112 specially, structural mechanisms controlling bleb dynamics are incompletely understood.

113 Ezrin is sufficient and important to sustain bleb dynamics for cell-in-cell invasion when SRF is supp

114 Both signals associate with bleb edges, as expected for membrane-localized proteins,

115 hat annexin overexpression promoted external blebs enriched in Ca2+ and correlated with a reduction o

116 ate in the direction of a very large 'leader bleb.' Eps8 bundling activity promotes cortex tension an

117 visual acuity (VA), mean deviation (MD), bleb evaluation, according to Moorfields Bleb Grading Sy

118 tokinesis, actin-dependent motility and cell blebbing, eventually abrogating gastrulation.

119 System (MBGS); spectral domain OCT (SD-OCT) bleb examination; number of glaucoma medications; freque

120 ation propagate faster than the timescale of bleb expansion and that pressure equilibrates slower tha

121 ip among cytoplasmic rheology, pressure, and bleb expansion dynamics, and provide a more detailed pic

122 ty and elasticity of the cytoplasm determine bleb expansion time.

123 he transition into a tube-flattening mode of bleb expansion while also predicting that the blebbing r

124 stored in tubes may serve as a reservoir for bleb expansion.

125 re equilibrates faster than the timescale of bleb expansion.

126 re equilibrates slower than the timescale of bleb expansion.

127 ilibration when compared to the timescale of bleb expansion.

128 mics have previously been used to understand bleb expansion.

129 cations (P = 1.000), bleb height (P = .625), bleb extension (P = .216), bleb vascularity (P = .672),

130 cy of trabeculectomy surgery with respect to bleb failure or IOP control was observed in both types o

131 Membranous blebs form as a cytotoxic reaction to lymphocyte attack.

132 role of physical forces in controlling where blebs form, and shows that in certain circumstances they

133 ng F-actin levels but blocking pseudopod and bleb formation and migration.

134 , E4orf4 induced a high incidence of nuclear bleb formation and repetitive nuclear ruptures, which pr

135 We induced bleb formation by disruption of the cortex and found tha

136 Bleb formation has been correlated with nonmuscle myosin

137 Bleb formation is independent of ubiquitin conjugation w

138 Bleb formation is ion specific, inducible by exposing th

139 -IIC) have the same or differential roles in bleb formation is not well understood.

140 mug/mL also inhibited cell growth and led to bleb formation on a fraction of the cells.

141 ongue with increasing sizes of intramyelinic bleb formation that could result in radial fractures of

142 f the invaginations strongly interfered with bleb formation, cell motility, and the ability of the ce

143 IC1-GFP-expressing MCF-7 cells show multiple bleb formation, compared with 36% of cells expressing GF

144 nt background results in a stark increase of bleb formation, even though Tor2A does not respond to LA

145 The kinetics of bleb formation, its dependence on POM121, and a reductio

146 llowing the inflation of the membrane during bleb formation.

147 n and intracellular pressure to drive leader bleb formation.

148 mpile an unbiased description of a "typical" bleb formed at the plasma membrane and quantify the effe

149 udies have relied heavily on recordings from blebs formed after axon transection, which may exaggerat

150 crostent and iStent Supra), and conjunctival bleb-forming procedures (Xen gel stent and InnFocus micr

151 emotaxing Dictyostelium cells preferentially bleb from concave regions, where membrane tension facili

152 by the narrow neck that separates the leader bleb from the cell body.

153 e adapted giant plasma membrane vesicles (or blebs) from native cell membranes expressing appropriate

154 hagocytic but not channel, pore, or membrane-blebbing function, and double-transfected P2X7L and P2X7

155 vely, the cornea became clear, the filtering bleb functioned well, and IOP returned to normal values.

156 for fibrosis and failure of the conjunctival bleb, further studies are required to explore the effect

157 Such findings have implications for blebbing, fusion, oxidation, and related properties of P

158 There was an insignificant difference in the bleb grading morphology regarding both groups (p = 0.35)

159 D), bleb evaluation, according to Moorfields Bleb Grading System (MBGS); spectral domain OCT (SD-OCT)

160 It was recently found that bleb growth is facilitated by a local supply of membrane

161 the jet injection method, and we modeled the bleb growth with an exponential saturation.

162 into three distinct phases: bleb nucleation, bleb growth, and bleb retraction.

163 Following nucleation and bleb growth, the actin cortex, comprising actin, cross-l

164 number of glaucoma medications (P = 1.000), bleb height (P = .625), bleb extension (P = .216), bleb

165 SD-OCT analysis denied differences in bleb height between MMC vs OLO (140.5 +/- 20.3 mu vs 129

166 Central bleb area, maximal bleb area, and bleb height were graded on a scale of 1 to 5 (1 indicati

167 4 (0.1) for central bleb area, 1.4 (0.1) for bleb height, and 3.4 (0.2) for bleb vascularity.

168 oid features such as cell rounding, membrane blebbing, high levels of contractility, and increased in

169 hrombin receptor PAR4 triggers cell membrane blebbing in a RhoA-and beta-arrestin-dependent manner.

170 A/myosin-II contractility to further augment blebbing in confinement.

171 We induced membrane blebbing in human HT1080 fibrosarcoma cells by inhibitin

172 r rigidity, which results in reduced nuclear blebbing in lamin B1 null nuclei.

173 The transition to blebbing in these conditions occurred through an interme

174 as well as nonapoptotic plasma membrane (PM) blebbing in this cellular motile process.

175 NM-IIB-GFP can form filaments in blebs in 41% of NM-IIB-GFP-expressing cells, whereas fil

176 ne, is sufficient to predict the location of blebs in rounded cells moving in a highly resistive envi

177 erature-dependent diffusion on cell membrane blebs, in cells depleted of cholesterol, and upon acute

178 Cells can also migrate using blebs, in which the edge is driven forward by hydrostati

179 retinal damage, particularly when SR vector bleb includes the fovea.

180 unction of Cdc42, and their unfolding allows bleb inflation and dynamic cell-shape changes performed

181 Because of their mechanical nature, blebs inform us about general cell-surface mechanics, in

182 oplasm shows that pressure disturbances from bleb initiation propagate faster than the timescale of b

183 gh an intermediate filopodial stage, whereas bleb initiation was biased toward filopodial bases, wher

184 ates calcium influx, directing movement with blebs instead of pseudopods.

185 ocular pressure (IOP) and the trabeculectomy bleb integrity, in a small series of eyes, both trabecul

186 ction, which results in reintegration of the bleb into the cellular cortex.

187 A bleb is initiated when the cytoskeleton detaches from th

188 Since nonapoptotic membrane blebbing is now recognized as an important regulator of

189 We find that cortical blebbing is tightly coupled to MRTF nuclear shuttling to

190 ion demonstrating that activation of MscL in blebs is identical to that in reconstituted bilayers.

191 Manipulation of blebs is sufficient to interfere with PCP and directed m

192 es of membrane protrusions, such as multiple blebs, lamellipodia, combinations of both, or absence of

193 hieve this, we generate HEK293 cell membrane blebs largely free of cytoskeleton.

194 The onset of bleb leak following trabeculectomy ranged from 7 months

195 Complete resolution of the bleb leak was observed following surgery in all cases.

196 Recurrent bleb leak was reported in 1 patient 2 years following in

197 Resolution of the bleb leak without the need for additional therapy or int

198 d the occurrence of complications, including bleb leak, hypotony, hyphema, choroidal effusion, choroi

199 ry glaucoma or juvenile glaucoma, history of bleb leak, intraocular pressure sustained below the targ

200 Michael's Hospital for bleb leaks repaired with MICS from 2012 to 2017.

201 Different stages of the bleb life cycle (expansion, pausing, and retraction) are

202 longer timescales that recapitulate the full bleb life cycle, including both expansion and healing by

203 M-II isoforms have differential roles in the bleb life cycle.

204 This excess is stored in a population of bleb-like protrusions (BLiPs), whose size distribution i

205 Irregular nuclear shapes characterized by blebs, lobules, micronuclei, or invaginations are hallma

206 ow PIEZO1 is activated by bilayer tension in bleb membranes, gating at lower pressures indicative of

207 lasticity and instigate a mode termed stable bleb migration in embryonic and tumor cells.

208 nvert border cells to a round morphology and blebbing migration mode.

209 resulted in comparable surgical outcomes and bleb morphologies.

210 , of post-TCP conjunctival changes on future bleb morphology and survival.

211 tion on intraocular pressure control and the bleb morphology in eyes that have undergone previous suc

212 wound, does not affect intraocular pressure, bleb morphology or function after one year of follow-up

213 Regarding the bleb morphology, the mean bleb morphology score in the temporal group was 10.50 +/

214 w-up regarding Intraocular pressure changes, bleb morphology score using the Wuerzburg bleb classific

215 uded the number of IOP-lowering medications, bleb morphology using the Indiana Bleb Appearance Gradin

216 raocular pressure and assessment of eventual bleb morphology variations in the follow-up period is ma

217 perative recovery of intraocular pressure or bleb morphology with or without the use of SPG-178.

218 Regarding the bleb morphology, the mean bleb morphology score in the t

219 ere bleb revision (32.1%) and intraoperative bleb needling (28.6%) and at any time postoperatively we

220 (28.6%) and at any time postoperatively were bleb needling (36.1%), bleb revision (23.9%), and tube s

221 When intraoperative bleb needling cases were excluded, the reoperation rate

222 Bleb needling may defer or avoid more invasive procedure

223 patients over the long term using a modified bleb needling technique, despite an average time from tr

224 be cis mutations in TEK/Tie-2 in blue rubber bleb nevus and sporadic vascular malformations.

225 Blue rubber bleb nevus syndrome (Bean syndrome) is a rare, severe di

226 E2, in 15 of 17 individuals with blue rubber bleb nevus syndrome.

227 T1105N-T1106P is recurrent in blue rubber bleb nevus, whereas Y897C-R915C is recurrent in sporadic

228 ition, sAC inhibition reversed BSIA membrane blebbing, nuclear condensation, and DNA fragmentation.

229 g can be divided into three distinct phases: bleb nucleation, bleb growth, and bleb retraction.

230 s, we establish that PAR4-dependent membrane blebbing occurs independently of the Galpha (q/11)- and

231 atform, we demonstrate that nuclear envelope blebbing occurs rapidly and synchronously immediately af

232 The model provides conditions under which blebbing occurs, and naturally gives rise to traveling b

233 misplacement and abnormal beating of cilia, blebbing of the microvilli.

234 WCR, injury to the midgut was manifested by "blebbing" of the midgut epithelium into the gut lumen.

235 eudopods can both power cell migration, with blebs often favored in tissues, where cells encounter in

236 is sufficient to increase nuclear volume and blebbing on two-dimensional surfaces, and acts synergist

237 analysis revealed the presence of pores and blebs on mutant-Lkt-treated PMNs.

238 ow the dermis), and cross-section (true skin bleb) perspectives and find that the top-view alone, as

239 rom mutant Krt75 mouse models reproduced the blebbing phenotype when grafted in vivo.

240 ransmembrane potential dissipation, membrane blebbing, phosphatidylserine exposure, DNA damage and ch

241 ent facilitation, membrane permeabilization, blebbing, phospholipid scrambling, inflammasome activati

242 Unlike blebbing, procoagulant ballooning is irreversible and a

243 that wild-type cells undergo a reduction in bleb protrusions near late gastrulation accompanied by a

244 leb expansion while also predicting that the blebbing rate is impaired by elevating the concentration

245 id (<10 s), sustained shift to movement with blebs rather than pseudopods.

246 is showed the significant risk factors for a bleb-related infection to be diagnoses of pigmentary gla

247 ion demonstrated a protective effect against bleb-related infections (P < .01) when risk factors were

248 d patients made aware of, the possibility of bleb-related infections long after trabeculectomy, espec

249 term follow-up demonstrates the incidence of bleb-related infections to be less than 2%, and describe

250 The Kaplan-Meier estimated incidence of bleb-related infections was 2.0% +/- 0.5% (mean +/- stan

251 Bleb-related infections were defined as blebitis, endoph

252 d describes the risk factors associated with bleb-related infections.

253 Twenty-four eyes were diagnosed with bleb-related infections; 15 eyes were found to have bleb

254 all cases, the initially repaired filtering blebs remained functional at last follow-up, and no addi

255 Here we show that MIIA specifically drives bleb retraction during cytokinesis.

256 MII then drives the final phase, bleb retraction, which results in reintegration of the b

257 MIIA at the cortex and its ability to drive bleb retraction.

258 MIIA both contribute to its ability to drive bleb retraction.

259 bout mechanisms of cytoskeleton assembly and bleb retraction.

260 y assemble the cytoskeleton, which can drive bleb retraction.

261 y the effect of Arp2/3 complex inhibition on bleb retraction.

262 ct phases: bleb nucleation, bleb growth, and bleb retraction.

263 postoperatively were bleb needling (36.1%), bleb revision (23.9%), and tube shunt implant (13.2%).

264 ost common reoperations within 180 days were bleb revision (32.1%) and intraoperative bleb needling (

265 Surgical bleb revision demonstrated a protective effect against b

266 , there was a higher unadjusted incidence of bleb revision in patients who had maculopathy (7.6 vs. 1

267 A protective effect of surgical bleb revision was demonstrated.

268 The fibrotic group had marked bleb scarring and vascularization and worse logMAR visua

269 Strikingly, numerous "bleb" shaped microprojections were observed extending fr

270 es or against mechanical resistance, but how bleb sites are selected and directed to the cell front r

271 f the cytoplasm relieves pressure and limits bleb size, and that both permeability and elasticity of

272 ly localizing components of the omega-shaped bleb structure.

273 We also find that traveling blebs tend to expand in all directions unless otherwise

274 ppreciation of physiological significance of blebbing, the molecular and, especially, structural mech

275 cancerous single-cells transitioning from a blebbing to a pseudopodial morphology.

276 he confines of tissues, cancer cells can use blebs to migrate.

277 Cells often employ fast, pressure-driven blebs to move through tissues or against mechanical resi

278 The model predicts conditions under which blebs travel or remain stationary, as well as the bleb t

279 travel or remain stationary, as well as the bleb traveling velocity, a quantity that has remained el

280 Furthermore, PM blebbing triggered SRF-mediated up-regulation of the met

281 Bleb-type cellular protrusions play key roles in a range

282 eight (P = .625), bleb extension (P = .216), bleb vascularity (P = .672), or complications rates.

283 Bleb vascularity was graded on a scale of 1 to 5 (1 indi

284 1.4 (0.1) for bleb height, and 3.4 (0.2) for bleb vascularity.

285 rrelation between mitochondrial dynamics and bleb vesicle formation using osmotic pressure stimuli in

286 y, and cell membrane-derived vesicles called blebs, Ward et al. visualize intermediates of the HIV-ce

287 A filtration bleb was seen that extended through the nasal 180 degree

288 for biased signaling in PAR4 since membrane blebbing was dependent on some, but not all, signaling p

289 apshots of fusion of the same particles with blebs were examined by cryo-ET.

290 -associated envelope abnormalities, that is, blebs, were confirmed by electron microscopy and immunof

291 15 patients, with otherwise well functioning blebs, were presented with uncontrolled IOP, in a variab

292 e each measurement before the cell undergoes blebbing, which is associated with a considerable increa

293 ost prominently in the formation of membrane blebs, which were shown to colocalize with incoming vira

294 tours, we use cell geometry to predict where blebs will form in migrating cells.

295 on should focus on generating an 8- to 10-mm bleb with each injection.

296 expression also affected nsPEF-induced cell blebbing, with only 20% of the silenced cells developing

297 ctive option for treating late-onset leaking blebs without cutting or excising conjunctival tissue.

298 A surgical technique to repair leaking blebs without incision or excision of conjunctiva is des

299 Six (30%) cystic thin avascular blebs without oozing were recorded in the MMC group and

300 atin results in a softer nucleus and nuclear blebbing, without perturbing lamins.