ライフサイエンスコーパス: blot

1 ays (fluorescent probe exclusion and Western blot).

2 ithm (enzyme immunoassay followed by Western blot).

3 horylation (Na(V)1.5, at serine 571, Western blotting).

4 s by immunohistochemistry but not by Western blot.

5 g test 7% presented an indeterminate Western blot.

6 included in the Methods section for Western Blot.

7 however it could not be detected by Northern blot.

8 and protein expression assessed with Western blot.

9 ative polymerase chain reaction, and Western blot.

10 n in colonic tissues was examined by western blot.

11 eatment as investigated by ELISA and western blot.

12 ay, caspase activity assay, FACS and Western blot.

13 gely relies on traditional ELISA and Western blot.

14 ls were quantified in hippocampus by Western blot.

15 nant Borrelia immunogenic protein A) Western blot.

16 d NPTX1 and NPTX receptor (NPTXR) by Western blot.

17 MS/MS analysis and some confirmed by western blot.

18 gets of miR-216a, was analyzed using western blot.

19 ared to that of untreated peanuts by western blot.

20 ative polymerase chain reaction, and Western blot.

21 only an ~50-kDa fragment appeared in Western blots.

22 l time quantitative PCR (RT-qPCR) or western blots.

23 levels using droplet digital PCR and Western blots.

24 4, and 6 (MVM, BM) was determined by Western blotting.

25 fers functionality to TLR4-levels by Western blotting.

26 Several changes can take place in wine after blotting.

27 H) were assessed by respirometry and Western blotting.

28 confirmed experimentally using tRNA Northern blotting.

29 bility, electrophoretic mobility shifts, and blotting.

30 ex and putamen were determined using western blotting.

31 s were independently validated using Western Blotting.

32 A (VEGF-A) were analyzed by means of Western blotting.

33 tors, was evaluated by Sambucus nigra lectin blotting.

34 -) mice after sepsis was assessed by Western blotting.

35 in the rinse samples was analyzed by Western blotting.

36 sfected HK-2 cells was determined by western blotting.

37 s further confirmed by both FRET and western blotting.

38 activation of NR4A1 was analyzed by western blotting.

39 phosphorylation was determined using Western blotting.

40 mass spectrometry (MS) or individual western blotting.

41 es, as measured by real-time PCR and western blotting.

42 ng a more quantitative read-out than Western blotting.

43 ve reaction for platelet factor-4 by Western blotting.

44 levels in Sprague-Dawley rats using western blotting, [(3)H]UCB-J autoradiography and immunostaining

45 tissue is confirmed by qRT-PCR and northern blot after oxidation/beta-elimination procedure.

46 uantitative real-time PCR (qPCR) and Western blot analyses confirmed that the levels of synaptotagmin

47 Western blot analyses indicated that sedentary rats had lower ex

48 Western blot analyses revealed drastic reduction of alpha-toxin

49 tein during immunogold-labeling- and western blot analyses, including relatively a greater membrane s

50 tative polymerase chain reaction and Western blot analyses, were performed to assess the potency and

51 chemical-, immunogold-labeling-, and western blot analyses.

52 stin, Arrb2) using RT-PCR, qPCR, and western blot analyses.

53 yme-linked immunosorbent assays, and Western blot analyses.

54 ugh transcriptome, RT-PCR, qPCR, and Western blot analyses.

55 Western blotting analyses revealed that 12(S)-HETE-lysophospholi

56 structural, immunohistochemical, and Western blotting analyses, we found that human and mouse amnion

57 NG pathway in the TME as assessed by western blot analysis and gene expression profiling.

58 ductal epithelial cells, as shown by Western blot analysis and mass spectrometry.

59 us retromer protein components using Western blot analysis and reverse transcription quantitative pol

60 qPCR, immunohistochemistry, and western blot analysis assessed intestinal Slc26a6 expression.

61 Western blot analysis confirmed significant inhibition of GLI1 a

62 Western blot analysis demonstrated a 3-5-fold increase in ATG9A

63 ing assay, Kim1 mRNA assessment, and Western blot analysis for cleaved caspase 3.

64 Western blot analysis further corroborated the MRM quantificatio

65 protein products were identified in western blot analysis in GLD1:II.3 and GLD2:II.2 (Fig.

66 protein products were identified in western blot analysis in GLD1:II.3 and GLD2:II.2 (Fig.

67 Western blot analysis of COS-1 cells transfected with recombinan

68 -isolated with EVs was determined by Western blot analysis of lipoprotein markers APOB and APOE.

69 Quantitative RT-PCR and Western blot analysis of sciatic nerve tissues showed that SC-Ex

70 ymerase Chain Reaction (qRT-PCR) and western blot analysis performed on (I) HEK293 stably transfected

71 Western blot analysis revealed significantly altered neurotrophi

72 The Western blot analysis revealed that CurDD increased Bax protein

73 Western blot analysis revealed that PFL treatment suppressed HER

74 ase (SERCA) activity was reduced and western blot analysis showed decreased or absent SERCA1 protein.

75 Western blot analysis showed that conditional KO of the miR-17-9

76 Western blot analysis showed that DPP6-L was dominantly expresse

77 ion tegument protein composition, as Western blot analysis shows a significant reduction in the tegum

78 Western blot analysis shows multiple SDS-stable assemblies in sy

79 Western blot analysis shows significant increase in rate-limitin

80 as mass spectrometry experiments and western blot analysis substantiated our approach.

81 chain reaction, flow cytometry, and western blot analysis that PMNs from FcgammaRIIIB WT donors and

82 Western blot analysis using anti-pan-serine/threonine antibodies

83 Western blot analysis was used to determine the protein levels o

84 rinergic receptors was determined by Western blot analysis, and mRNA expression was analyzed by micro

85 ophin protein content was evident by Western blot analysis, at three months post excision in skeletal

86 products were conducted by western blot, dot blot analysis, Raman spectroscopy, atomic force microsco

87 Protein expression was determined by Western blot analysis, using two antibodies for each HLA, specif

88 Using flow cytometry and Western blot analysis, we observed that blocking Ship1/2 abrogat

89 lectivity, and reproducibility using Western blot analysis.

90 lidated by coimmunoprecipitation and Western blot analysis.

91 chnology on the Luminex platform and western blot analysis.

92 ated fat necrosis was analyzed using Western blot analysis.

93 Signaling was studied by Western blot analysis.

94 Western blotting analysis detected hot spots on 67-kDa proteins

95 Western blot and confocal microscopy analysis revealed degradati

96 specific PLA(2)R1 antibody levels by western blot and ELISA.

97 in colon cancer tissues confirmed by western blot and IHC.

98 aluated for specificity, followed by Western blot and immunofluorescence analysis on islets from 10 a

99 1S-K(v)11.1 trafficking, as shown by Western blot and immunofluorescence microcopy analysis.

100 Western blot and immunofluorescence microscopy revealed the pres

101 11.1 trafficking, as demonstrated by Western blot and immunofluorescence microscopy.

102 ell as reverse-phase protein arrays, western blot and immunofluorescence staining were used to evalua

103 Western blot and immunofluorescence were performed for MTOC and

104 rvillin isoforms in muscle fibres by western blot and immunohistochemical analyses.

105 Western blot and immunohistochemical analysis of tissue from tem

106 unoprecipitation, mass spectrometry, Western blot and immunohistochemistry, we found that the target

107 Western blot and immunostaining analyses of AV nodes showed that

108 4 in gingival tissue was assessed by western blot and localization by immunohistochemistry.

109 re analyzed in corneal lysates using Western blot and Luminex assays.

110 d mitophagy markers were examined by western blot and mitochondrial biogenesis was inferred from Mito

111 assessed CGG repeat allele size by Southern Blot and PCR analysis.

112 Analyses by RNA gel blot and polysome association suggest that the tRNA defi

113 Using dot-blot and primer extension assays, we measured the suscep

114 athway of TLR4 was investigated with Western blot and proteomic analysis.

115 Using immunostaining, western blot and qPCR methods, we firstly identified that ITCH w

116 and mRNA expression were measured by Western blot and quantitative PCR analyses, respectively, and in

117 d NKCC1 levels were determined using Western blot and quantitative polymerase chain reaction analyses

118 ular analysis of exosomes, including Western blot and quantitative polymerase chain reaction.

119 ormation and removal from the genome by slot blot and repair capacity in an excision assay, and used

120 MARCO, and p-CREB were measured with western blot and RNAseq.

121 ogical diagnosis was confirmed using Western blot and specific polymerase chain reaction with sequenc

122 and CYP4A protein expression, using Western blot and then compared to PFAA concentrations in liver a

123 neocortex (CX) were determined with western blots and compared between MTLE-HS patients who were sho

124 t least 10 times more sensitive than Western blots and could detect quantitative changes in protein e

125 patient sera with fibrinogen in both western blots and ELISAs.

126 tein assays including mass spectrometry, dot blots and Western blotting were developed to determine t

127 Moreover, western blotting and confocal microscopy revealed that LH stimul

128 in tumor cells by 50% as measured by Western blotting and flow cytometry.

129 Here we use western blotting and immunofluorescence to examine the effects o

130 d primary human hepatocytes (PHH) by Western blotting and immunofluorescence.

131 cells using a SMAD luciferase assay, Western blotting and Immunohistochemistry.

132 ut of HEK 293 and A549 cells by both Western blotting and lipid mass spectrometry, we observed dimini

133 Our Western blotting and messenger RNA expression data demonstrated

134 performance leads to variability in Western blotting and other immunoassays.

135 Western blotting and PCR were used to assay changes in EC coupli

136 ere analyzed by immunosorbent assay, Western blotting and quantitative PCR.

137 esponding to ECM were measured using Western blotting and quantitative RT-PCR with subsequent matrix

138 abnormal prion protein (PrP(Sc)) by western blotting and real-time quaking-induced conversion (RT-Qu

139 ntioxidant system was carried out by Western blotting and showed sex-related differences.

140 n levels of the targeted proteins by Western blotting and used quantitative microscopic assays to con

141 molecules were measured for protein (Western blot) and messenger RNA (quantitative reverse transcript

142 measurements at both the cell population (by blotting) and single-cell (by imaging) levels.

143 f the lungs, followed by the RT-PCR, western blot, and confocal microscopy, was performed.

144 (Chiu score, Alcian blue staining), Western blot, and electrophysiological assessment (Ussing chambe

145 esophageal cancer cells was shown by Western blot, and esophageal cancer cells were able to induce ne

146 ransmission electron microscopy, and Western blot, and EV-associated miRNAs were identified by a RT-q

147 ation was verified by PCR analyses, Southern blot, and germ-line transmission.

148 s of male and female mice using PCR, Western blot, and immunofluorescence staining.

149 tion and to perform flow cytometric, Western blot, and immunohistochemical tumor analyses.

150 ain glycoproteins by flow cytometry, Western blot, and immunohistochemistry.

151 s enzyme-linked immunosorbent assay, Western blot, and lateral flow assays.

152 e enzyme-linked immunosorbent assay, Western Blot, and mass spectrometry have enabled discovery and s

153 Control and Preeclampsia subjects by Western blot, and overall, lower expression was noted in samples

154 correlate well with cell viability, western blot, and qPCR data.

155 nocytochemistry, immunofluorescence, Western blot, and RT-PCR were performed in highly purified perip

156 by immunoelectron microscopy (IEM), Western blots, and enzyme activities.

157 re mislabelled when selecting representative blots, and were erroneously duplicated from GluA2.

158 ins were resolved by gel electrophoresis and blotted, and Siglec-8 ligands detected.

159 ethods such as immunohistochemistry, western-blotting, and also by enzyme-linked immunosorbent assay.

160 uantitative PCR, immunofluorescence, Western blotting, and both chromogenic and single-molecule in si

161 ioenergetics utilizing trypan blue, Southern blotting, and extracellular flux analysis, respectively.

162 termined by luminescence-based plate assays, blotting, and imaging.

163 s studied using immunoprecipitation, Western blotting, and immunofluorescence in NIH/3T3 cells transf

164 re assessed by qPCR, flow cytometry, Western blotting, and immunofluorescence.

165 cells was analyzed by real-time PCR, western blotting, and immunohistochemical staining.

166 physiological, immunohistochemistry, Western blotting, and patch clamping of membrane potentials was

167 by histology, immunohistochemistry, western blotting, and polymerase chain reaction.

168 In situ activity assays, Western blotting, and quantitative PCR were used to investigate

169 The Geenius and HIV-1 Western blot assay results were negative or indeterminate for 73

170 -induced recombinant tau oligomers and a dot blot assay, we discovered a mAb (M204) that binds oligom

171 his hypothesis, we performed EYS Far-Western blotting assay and generated pomgnt1 mutant zebrafish.

172 med by the Geenius HIV 1/2 and HIV-1 Western blot assays (Bio-Rad).

173 tative polymerase chain reaction and Western blot assays in fresh human tissues (n = 8) and primary c

174 transcription-quantitative PCR, and Western blotting assessments showed that VSV-EBOVDeltaMLD produc

175 of M and S opsin were quantified by western blotting at 10 weeks.

176 LTL was measured by Southern blotting at baseline and ~14 yr thereafter.

177 corrected Figure S1 with the correct western blot bands of Na-K ATPase.The authors would like to apol

178 ication and assessed with chemical-probe and blot-based assays for cysteine S-nitrosation, sulfenylat

179 MS-based protein identification and Northern blotting-based rRNA detection approaches identified two

180 reported mutations were compared by Western blotting, Ca2(+) flux assays, differentiation of transdu

181 ERCA activity measurement and SERCA1 western blot can assist in proving the pathogenicity of novel AT

182 analysis of proteins of interest by western blotting can be completed within 1-2 weeks.

183 iver histology, gene expression, and western blot characterization of BA and FA synthesis/transport.

184 Immunofluorescence and Western blot confirmed PTH1R expression in arterial VSM that was

185 Kv3.3 immunostaining was present and western blots confirmed loss of subunit protein in the respectiv

186 Subsequent immunoprecipitation/Western blots confirmed the endogenous interaction in HCT116, 29

187 ional approaches, cell transfection, Western blot, confocal microscopy, cell degranulation, prostagla

188 This blot corresponds to the p-Rb panel, as can be seen in th

189 We demonstrated that the new dot blot coupled to biotin-Si-NPs successfully detected Camp

190 ion/invasion and preeclampsia, while Western Blot data showed the activation of HIF-1alpha, but not N

191 ations, with available muscle biopsy Western blot data, were included irrespective of whether they pr

192 Western blotting demonstrated higher levels of inflammatory fact

193 Western blotting demonstrated that Muller cell-specific AQP4 was

194 Western blot detected several specific uEV kidney and EV markers

195 tive in all CJD PNS samples, whereas western blotting detected PrP(Sc) in the sciatic nerve in one VV

196 Cortical tissue was collected for Western blot detection of astrocyte and microglial activation (G

197 Western blotting determined P-gp and BCRP protein levels.

198 f RT-QuIC products were conducted by western blot, dot blot analysis, Raman spectroscopy, atomic forc

199 Using immunofluorescence assays and Western blotting during infection in cell culture and in chicken

200 tion and DNA repair were analyzed by western blotting, electrophoretic mobility-shift assay, and immu

201 Western blots, ELISAs, and confocal immunocytochemistry showed E

202 oduced during the standard technique used to blot EM grids and that these manifest in nonuniform ice

203 ein levels were further confirmed by Western blot, enzyme-linked immunosorbent assay and immunohistoc

204 roducible results within and between Western blotting experiments and the observed effect confirmed w

205 We corroborated by western blotting experiments that PTPN14 and CAV1 co-inmunopreci

206 mastigote excretory secretory antigen (TESA) blot for detection of immunoglobulin M (IgM)-specific sh

207 The IgM TESA blot for detection of SAPA bands is rapid, relatively in

208 The detection limit of Western blot for low-abundance PEGylated interferon (Pegasys) an

209 ALDH/CD44 (CSC markers for MEC), and Western blots for Bmi-1 expression (marker of stem cell self-ren

210 elay lines that enables automated, fast, and blot-free sample vitrification.

211 Northern blots from skeletal muscle total RNA showed severe reduc

212 Blotting has been the standard technique for preparing a

213 applied a combination of molecular (Southern blot hybridization and reverse-transcription cleaved amp

214 dynamic chemistry (ChemNAT) with reverse dot-blot hybridization in an array format for a rapid and ea

215 Further analysis was performed by Western blot, immunohistochemistry, real-time polymerase chain r

216 scription polymerase chain reaction, Western blotting, immunohistochemistry, and fluorometric assays.

217 arian tissue by quantitative PCR and Western blot/immunohistochemistry, respectively.

218 expression levels were determined by Western blot in HEK293T cells transfected with variant or wild-t

219 be seen in the unprocessed version of these blots in Supplementary Fig.

220 abel 'IP-PBP3' above the second of the three blots incorrectly read 'IP-PBP1B'.

221 Using Western blotting, kinetic assays, and microfluidic analyses, we

222 A (quantitative RT-PCR) and protein (western blotting) levels.

223 nd reporting in immunoassays such as Western blotting may promote improved reproducibility across the

224 nitiative in 1993-1998 with data on Southern blot-measured LTL and self-reported usual sleep duration

225 lected at birth for morphometric and Western blot measurements of HIF-1a, HIF-2a, VEGF (vascular endo

226 rescently labeled chelator heads in PAGE and blot membranes.

227 ds to detect His-tagged proteins in PAGE and blot membranes.

228 -5 orders of magnitude from conventional Dot-blot methods.

229 Western blot monitoring of teichoic acid production revealed dif

230 ase chain reaction (n=4-6/group) and Western blot (n=3-4/group).

231 s of high purity, as demonstrated by Western Blots of compartment-specific marker proteins.

232 mparison of these results with redox Western blots of Prx3 and Prx2 oxidation states demonstrated rea

233 tectable by both inverse RT-PCR and northern blotting of HPV16-transformed cells.

234 ch cell in the microwell and perform western blotting on each resultant lysate.

235 for MPC2 in click chemistry-enabled western blotting or global mass spectrometry-based proteomic exp

236 2d, the Western blot panels representing GAPDH endogenous loading contro

237 Hence, such an enhanced dot blot paves the path to the development of a portable and

238 As shown by immunohistochemistry and Western blotting, PPARalpha was downregulated in the corneas of

239 s were screened for HO-1 expression (Western blots) prior to put-in (basal) and post reperfusion (str

240 assage, relative incubation periods, western blot profiles, and neuropathology were maintained.

241 were here integrated with optimized western blotting protocols in solving the complex IEF pattern of

242 Confocal microscopy and western blotting provided evidence of the formation of a covalen

243 The feasibility of Quantitative Dot Blot (QDB) method for this purpose was explored in this

244 Echocardiography, Western blotting, qPCR, immunohistochemistry, immunofluorescence

245 escence and confocal microscopy, and western blotting quantification of the protein phosphorylation w

246 field and rotarod tests, followed by Western blotting, quantitative PCR, and immunohistochemistry to

247 d using Illumina multiplex Elisa and western blot, respectively.

248 was measured using real-time PCR and western blotting, respectively.

249 Western blotting results also revealed decreased protein express

250 amate-induced calcium elevations and Western blots reveal ionotropic glutamate receptor expression pr

251 A highly sensitive sandwich Western blot revealed read-through translation of Igh (Ter5H) me

252 Southern blotting revealed btl genes in 14 diverse Mycetohabitans

253 Western blotting revealed that protein expression of TLR4 was ma

254 resonance energy transfer (FRET) and western blotting revealed the activation of Src in ECs was signi

255 Signaling pathways were examined by Western blot, RNA-seq, and qPCR.

256 BECs and AVM-BECs were determined by Western blot, RT-qPCR (quantitative reverse transcription polyme

257 iptase polymerase chain reaction and Western blot showed a marked reduction of protein expression.

258 Western blotting showed expression of sialin, a known nitrate tr

259 end our RNA sequencing results with northern blots, showing that tRNAs and Y RNAs in the non-vesicula

260 Protein blots stained with nitroblue tetrazolium (NBT) showed in

261 icin degradation was monitored using western blot, staining by Coomassie or Periodic Acid-Schiff base

262 Quantitative RT-PCR and Northern blotting studies showed reduced or loss of expression of

263 Northern blotting suggests that these anti-CRISPRs manipulate sin

264 related with optical micrographs and western blot tests.

265 On Western blotting, the mean ratios of pNFKB:NFKB for control and

266 Contrary to common Western blotting, the separation occurs in nondenaturing conditi

267 iChemR23 was examined by qRT-PCR and Western blotting.The roles of the MAPK and PI3K-Akt signaling pa

268 .3 fg or ~10(4) molecules, whereas the total blotting time is about 5 min.

269 used immunofluorescence staining and western blot to test the transduction efficiency of this vector.

270 We used RNA sequencing and Western blotting to analyze the signaling pathways regulated by

271 s, fast scan cyclic voltammetry, and western blotting to examine whether sleep/wake state and/or ligh

272 (Ser235/Ser236) was performed using Western blotting to infer the degree of activation of AMPK signa

273 we have used immunoprecipitation and Western blotting to show that in response to LPS, LPCAT2, but no

274 P-Wb (allele-imbalanced DNA pulldown-Western blot) to detect allele-specific protein:fSNP binding.

275 and the C2C12 cell line for utrophin western blots, to independently evaluate the site blocking effic

276 otein acetylation levels in cells by western blotting (tubulin vs histone acetylation), and by assess

277 ab (Charybdis feriatus) allergens by western blot using patients' sera.

278 duced as shown by flow cytometry and Western blotting, using strains expressing different glutamine l

279 Western blot validation showed overexpression of ANXA2 and CDC42

280 When compared to qPCR, IgM TESA blot was both sensitive and specific for congenital Chag

281 f this Article originally published the same blot was inadvertently presented as both p-Rb and Cyclin

282 Dot blotting was also used to analyze expression changes in

283 Additionally, quantitative Western blotting was carried out for pNFKB and NFKB in specimens

284 Western blotting was carried out to investigate expression level

285 Western blotting was performed to measure levels of total-Tau, p

286 ell lines (Kelly and SK-N-BE(2)C) by Western blot (WB) and immunohistochemistry.

287 The utility of Western blotting (WB) for the serodiagnosis of canine bartonello

288 To improve the response of standard dot blots, we have applied a new enhancement strategy that i

289 orescence activated cell sorting and Western blot were performed in patient-derived fibroblasts and m

290 ce and in vivo electrophysiology and Western blots were conducted in rats subject to fluid percussion

291 ong immunoreactivity around 70kDa on Western blotting were also anti-Drebrin-positive.

292 assay, flow cytometry analysis, and Western blotting were applied to evaluate the activation of rela

293 ing mass spectrometry, dot blots and Western blotting were developed to determine the level of Ara h

294 qPCR and western blotting were used to assess ion channel expression.

295 , regulation of 15-PGDH, RT-PCR, and Western blot, were performed using peripheral blood from healthy

296 idate the in situ scWB with slab-gel western blot, while revealing cell-to-cell heterogeneity in stre

297 Western Blotting with anti- WFhb1-1 antibody revealed a signific

298 r binding partners were determined by ligand blotting with CLC/Gal-10, followed by coimmunoprecipitat

299 Western blotting would be a simple end point detection method fo

300 Cell viability, Western Blotting, Zymogram, and Real-time PCR analyses were perf