ライフサイエンスコーパス: budding of

1 es into the PSAP region of M protein rescued budding of a PPPY mutant of VSV to wild-type levels.

2 Thus, proteasome inhibitors can affect the budding of a virus that assembles within the cytoplasm.

3 is critical region uniformly resulted in the budding of abnormal, long tubular particles.

4 it is rendered PTAP or PPPY dependent; (iii) budding of all EIAV clones is blocked by dominant negati

5 s Y400 and L402 in cdE2 as important for the budding of alphaviruses.

6 In pancreatic beta-cells, the budding of AP-1/clathrin-coated vesicles, a portion of w

7 the cell, just as epsin1 is involved in the budding of AP2 CCVs.

8 ly reducing WAVE1 in N2a cells decreased the budding of APP-containing vesicles and reduced cell-surf

9 Plasma membrane budding of Atg-16L-positive vesicles represents a very e

10 The budding of avian sarcoma leukosis virus and HIV-1 Gag vi

11 Budding of bald FIV and HIV particles was blocked by car

12 tein, which might influence the assembly and budding of BDV.

13 ependent of its ability to promote vesicular budding of beta-amyloid precursor protein.

14 t not catalytically inactive, PLD1 increases budding of betaAPP-containing vesicles from the trans-Go

15 Budding of biotin-tagged SFV was continuous for at least

16 -ribosylation factor appears to regulate the budding of both COPI and clathrin-coated transport vesic

17 G101/ESCRT-I activity and thereby rescue the budding of both mutant Gag particles and HIV-1 viruses l

18 inal fragment of TSG101 (TSG-3') blocked the budding of both PTAP-dependent and PPPY-dependent retrov

19 in disrupted 14-3-3 binding and improved the budding of both virus-like particles (VLPs) and recombin

20 Budding of C-type retroviruses begins when the viral Gag

21 ty not only inhibits GTP-induced fission and budding of caveolae but also prevents caveolae-mediated

22 ET-A inhibitor BQ123, blocks the ET-induced budding of caveolae.

23 This IBS also impairs the budding of CD63 and several other viral and nonviral EMV

24 g, regulation of the immune response and the budding of certain enveloped viruses such as human immun

25 lts suggest that ankyrin plays a role in the budding of clathrin-coated pits during endocytosis.

26 reported that annexin VI is required for the budding of clathrin-coated pits from human fibroblast pl

27 We found that when the budding of clathrin-coated vesicle is blocked without si

28 e mutant of dynamin, a gene required for the budding of clathrin-coated vesicles (CCVs).

29 ut by the assembly of clathrin coats and the budding of clathrin-coated vesicles from the neuronal pl

30 inhibition of dynamin, a GTPase required for budding of clathrin-coated vesicles from the plasma memb

31 The large GTPase dynamin is required for budding of clathrin-coated vesicles from the plasma memb

32 ly associated with the trans-Golgi where the budding of clathrin-coated vesicles occurs.

33 In contrast, when budding of clathrin-coated vesicles was blocked at the p

34 Amantadine, which blocks the budding of clathrin-coated vesicles, inhibited phagocyto

35 During the budding of coat protein complex II (COPII) vesicles from

36 to the medial cisternae, and do not inhibit budding of COP-coated vesicles, but do accumulate docked

37 Budding of COPI-coated vesicles from Golgi membranes req

38 ndoplasmic reticulum is brought about by the budding of COPII vesicles.

39 c lipids and that this assembly promotes the budding of COPII-coated vesicles.

40 ly of new virions is thought to occur by the budding of core viral particles into a late secretory pa

41 The strong bias toward distal budding of daughter cells requires the distal-pole tag B

42 ociated with activation of senescence, while budding of daughter cells was associated with senescence

43 the cell cortex; in particular, the bipolar budding of diploid cells depends on persistent landmarks

44 This leads to the budding of disc-derived vesicles, specifically at the si

45 To determine whether budding of DNA-containing HBV virions at intracellular m

46 strate that seipin is required for vectorial budding of droplets toward the cytoplasm.

47 of human and murine tetherin and facilitated budding of Ebola particles, as did the HIV-1 Vpu protein

48 Budding of Ebola virus (EBOV) particles from the plasma

49 wild-type vps4, significantly inhibited the budding of Ebola virus (Zaire).

50 Interestingly, we found that budding of Ebola virus VLPs was more pronounced in TLR4-

51 SGylation system (UbE1L and UbcH8), inhibits budding of Ebola virus VP40 VLPs.

52 and that expression of human SOCS3 enhances budding of Ebola VLPs and infectious virus via a mechani

53 mother cell wall, a type of ECM, through the budding of ectosomes directly from the membranes of its

54 in the p9 protein on its ability to mediate budding of EIAV Gag particles.

55 s4, and proteasome inhibitors to disrupt the budding of EIAV particles bearing each of the three type

56 erminal TSG101 fragment potently impairs the budding of EIAV when it is rendered PTAP or PPPY depende

57 We also examined the role of NC in the budding of EIAV, a retrovirus relying exclusively on the

58 the p38 alpha and p38 beta isoforms suppress budding of embryonic mouse lung explants and isolated en

59 a mechanistic explanation for the polarized budding of EMVs and retroviruses, including HIV.

60 Endophilins A1 and A2 promote the budding of endocytic vesicles from the plasma membrane,

61 topic expression of E-cadherin also impaired budding of endoderm in vitro.

62 The budding of endoplasmic reticulum (ER)-derived vesicles i

63 le formation at the multivesicular body, the budding of enveloped RNA viruses such as HIV-1, and the

64 ecessary and sufficient for the assembly and budding of enveloped virus-like particles from the cell.

65 ies implicate rafts as sites of assembly and budding of enveloped virus.

66 The ESCRTs are also needed for budding of enveloped viruses including human immunodefic

67 body sorting, abscission during cytokinesis, budding of enveloped viruses, and repair of the plasma m

68 ular processes such as assembly of caveolae, budding of enveloped viruses, and sorting of lipids and

69 of vesicles into the lumen of endosomes, the budding of enveloped viruses, and the separation of cell

70 ompared with the Brownian simulations of the budding of enveloped viruses.

71 Here we show that the budding of equine infectious anemia virus (EIAV) from in

72 P egress and that Amot co-expression rescues budding of eVP40 VLPs in a dose-dependent and PPxY-depen

73 ell receptor (TCR) ligands and ICAM-1 induce budding of extracellular microvesicles enriched in funct

74 of the viral matrix protein (VP) 40 to drive budding of filamentous particles that can also incorpora

75 When expressed alone, VP40 induces budding of filamentous virus-like particles, suggesting

76 with others have established that efficient budding of filoviruses, arenaviruses, and other viruses

77 Budding of filoviruses, arenaviruses, and rhabdoviruses

78 lar protein Alix is sufficient to rescue the budding of FIV mutants devoid of canonical L-domains.

79 As such, budding of Gag from yeast cells appears to represent ESC

80 Furthermore, in vitro budding of GLUT4 vesicles but not GLUT1 or the transferr

81 mechanism of eHAV egress involving endosomal budding of HAV capsids into multivesicular bodies.

82 intracellular multivesicular body, where the budding of HBV virions takes place.

83 ins) play critical roles in the assembly and budding of herpesviruses.

84 orting machinery that has been implicated in budding of HIV and Ebola virus.

85 Efficient budding of HIV from the plasma membrane requires a small

86 We propose a model for the budding of HIV virions through lipid rafts whereby host

87 of membrane necks in processes including the budding of HIV-1 and cytokinesis.

88 sicular bodies in endolysosomal sorting; the budding of HIV-1 and other viruses from the plasma membr

89 for cytokinesis in animal cells, and for the budding of HIV-1 from human macrophages and T lymphocyte

90 Efficient budding of HIV-1 from the plasma membrane of infected ce

91 ic vesicles, in contrast to the plasmalemmal budding of HIV-1 typically seen with infected T cells.

92 Interestingly, budding of HIV-2 virus-like particles from cells was enh

93 ns ranging from lysosomal degradation to the budding of HIV.

94 tor downregulation, lysosome biogenesis, and budding of HIV.

95 ult suggests that Nedd4 is involved early in budding of HTLV-1.

96 oviral budding from the plasma membrane, and budding of human immunodeficiency virus type 1 (HIV-1) i

97 Proteasome inhibitors reduce the budding of human immunodeficiency virus types 1 (HIV-1)

98 ) interactions are necessary to initiate the budding of individual membrane vesicles, the dependence

99 ortant for proper virus assembly and for the budding of infectious particles.

100 ng, but the protein interactions that govern budding of infectious virus are not known.

101 e particles (VLPs) that accurately mimic the budding of infectious virus.

102 ins, it has been proposed that the polarized budding of influenza virions depends on the interaction

103 atrix protein M1 plays a pivotal role in the budding of influenza virus from the plasma membrane (PM)

104 of M2-mediated membrane scission during the budding of influenza viruses.

105 quire expression of the envelope protein for budding of intracellular capsids from the cell, suggesti

106 ires expression of the envelope proteins for budding of intracellular M1 into virus particles.

107 RT-III executes membrane scission during the budding of intralumenal vesicles (ILVs) at endosomes.

108 In eukaryotic cells, the budding of intraluminal vesicles (IVLs) is mediated by t

109 describe a prototype therapeutic that blocks budding of JUNV and has the potential to function as a b

110 l-free assay to recapitulate COPII-dependent budding of large lipoprotein cargoes from the ER.

111 cating that GTP-bound LdSar1 is required for budding of Ldgp63-containing COPII vesicles.

112 brane (FIT) proteins, is required for proper budding of LDs from the ER.

113 The budding of LDs requires extensive ER-LD crosstalk, but h

114 interactions required for HIV-1 assembly and budding of lentivirus particles.

115 Budding of lentiviruses occurs at the plasma membrane, b

116 The assembly and budding of lentiviruses, such as human immunodeficiency

117 the isolated V domain in vitro and impaired budding of lentiviruses.

118 (VLPs) by a mechanism that accurately mimics budding of live virus.

119 s, the lymphatic vascular system develops by budding of lymphatic progenitor endothelial cells from e

120 th in endosomal vesicle formation and in the budding of many enveloped RNA viruses, including HIV-1.

121 The budding of many enveloped RNA viruses, including human i

122 transport (ESCRT) machinery is necessary for budding of many enveloped viruses.

123 Ubiquitin is important for the budding of many retroviruses and other enveloped viruses

124 Using PPxY-dependent budding of Marburg (MARV) VP40 virus-like particles (VLP

125 ace may be useful in inhibiting assembly and budding of MARV.

126 step in cellular-trafficking pathways is the budding of membranes by protein coats, which recent expe

127 f viral components at cellular membranes and budding of membranes to release particles.

128 ic cellular mechanism that results in direct budding of microvesicles from the plasma membrane, provi

129 rom a characteristic pattern of branching or budding of modules, which may remain attached or become

130 However, the budding of mouse mammary tumor virus (MMTV; cytoplasmic

131 und that proteasome inhibition decreased the budding of murine leukemia virus (plasma membrane assemb

132 with mVP40-DeltaLPLGIM successfully rescued budding of mVP40-DeltaLPLGIM into VLPs at mVP40-WT level

133 d that FIT proteins are necessary to promote budding of nascent LDs from the ER.

134 ons demonstrate that pro-SRIF processing and budding of nascent secretory vesicles from the TGN can b

135 tors pervanadate and zinc potently inhibited budding of nascent secretory vesicles.

136 ix, and the RNPs that presumably control the budding of nascent virions from host cells.

137 We found that budding of new boutons at Syn(-) NMJs was significantly

138 at sites of synaptic growth and facilitates budding of new boutons via a cAMP/PKA-dependent pathway.

139 Additionally, neogenesis or the budding of new islet cells from pancreatic ducts has bee

140 esicle-Associated Membrane Protein-2 and (c) budding of new recycling sites from previously existing

141 ith endosome-like compartments and, finally, budding of new synaptic vesicles from endosomes, althoug

142 tments of the host cell for the assembly and budding of new virion particles.

143 Thus, NAK could participate in the budding of new virions, the modification of viral protei

144 with the protein's role in facilitating the budding of new virus particles from infected cells.

145 s ALIX and HD-PTP play a crucial role in the budding of newly assembled capsids into multivesicular e

146 irus morphogenesis that involves a transient budding of newly made immature viral particles into the

147 irus Nipah virus (NiV), we have examined the budding of NiV M.

148 th the defect being attributed mainly to the budding of noninfectious particles.

149 portion of TSG101 (TSG-3') potently inhibits budding of not only HIV-1 but also murine leukemia virus

150 ovide evidence that MSP export occurs by the budding of novel vesicles that have both inner and outer

151 o help modify the nuclear lamina and promote budding of nucleocapsids at the inner nuclear membrane.

152 (BV) into the host cell and (ii) egress and budding of nucleocapsids newly produced from the plasma

153 ena such as perturbation growth, necking and budding of offspring droplets from a bulk body are obser

154 or depletion of cellular TSG101 reduced the budding of only M40-containing VLPs but not that of wt M

155 Wnt signaling may be a common theme for the budding of organ anlagen from the endoderm.

156 stigate this effect further, we examined the budding of other retroviruses from proteasome inhibitor-

157 a related sequence, FPIV, important for the budding of parainfluenza virus 5.

158 Matrix (M) proteins reportedly direct the budding of paramyxoviruses from infected cells.

159 ding the spike (S) glycoprotein, facilitated budding of particles that contained a corona-like halo r

160 We found that FABP5 promotes the budding of particles ~150 nm in diameter and modulates t

161 y of coexpressed wt HN protein to direct the budding of particles.

162 s that regulates segregation, packaging, and budding of peroxisomal importomer subcomplexes, thereby

163 an colonies normally develop through asexual budding of polyps that remain interconnected by continuo

164 Ypt1p to mediate intra-Golgi traffic or the budding of post-Golgi vesicles from the trans-Golgi.

165 functions as an Arf4 effector that regulates budding of post-TGN carriers, along with FIP3 and Rab11.

166 al RNA with C protein into nucleocapsid, and budding of prM and E proteins into virions.

167 f influenza virus proteins necessary for the budding of progeny virions needs to accumulate at budozo

168 rus play a critical role in the assembly and budding of progeny virions.

169 us (RV) play a key role in both assembly and budding of progeny virions.

170 he mouse-adaptive changes did not affect the budding of RAVV VP40 in mouse cells, suggesting that thi

171 Budding of retroviruses and some other enveloped RNA vir

172 , our studies establish a role for NC in the budding of retroviruses harboring divergent L domains an

173 y, we examined a possible role for NC in the budding of retroviruses relying on divergent L domains a

174 Budding of retroviruses requires the structural precurso

175 During assembly and budding of retroviruses, host cell proteins are incorpor

176 mportant role in filovirus budding, and that budding of retroviruses, rhabdoviruses, and filoviruses

177 Calculations demonstrate that the budding of reverse micelles formed from interfacial Sr(I

178 host cell proteins that regulate directional budding of RSV are undefined.

179 important for efficient genome packaging or budding of RSV from the infected cell.

180 Ppl stimulated budding of RTCs, but blocked membrane delivery to the RO

181 Assembly and budding of SARS-CoV-2 from the host ER-Golgi intermediat

182 e data localize the sterol-regulated step to budding of SCAP from ER and provide a system for biochem

183 ein kinase D1 (PKD) that specifically blocks budding of secretory vesicles from the TGN and does not

184 rather than DAG is a key step in regulating budding of secretory vesicles from the trans-Golgi netwo

185 uantitative biochemical assay to monitor the budding of Semliki Forest virus (SFV), an enveloped alph

186 ns into multivesicular bodies (MVBs) and the budding of several enveloped viruses, including HIV-1.

187 s for signal transduction and as the site of budding of several enveloped viruses, including influenz

188 ir conservation and importance for efficient budding of several negative-stranded RNA viruses.

189 of < or =7.0, conditions that inhibited the budding of SFV but not the budding of the rhabdovirus ve

190 at E2 position 209 is required for complete budding of Sindbis virus particles although several diff

191 tinuous with the plasma membrane; incomplete budding of smaller vesicles from 100nm vesicles further

192 plays an essential role in the assembly and budding of some enveloped RNA viruses.

193 dimer-forming leucine zipper domain restores budding of spherical particles morphologically similar t

194 domains that cause membrane deformation and budding of spherical vesicles, as seen by fluorescent an

195 s a cellular antiviral factor that restricts budding of structurally diverse enveloped viruses.

196 both M and F proteins are able to drive the budding of SV and propose the possible role of actin in

197 roline in the SV5 M protein resulted in poor budding of SV5 VLPs and failure of recombinant SV5 virus

198 te further the requirements for assembly and budding of SV5, we generated two double-mutant recombina

199 The involvement of ARF proteins in the budding of SVs was addressed in a cell-free reconstituti

200 Specifically, we observed defects in the budding of synaptic vesicles from the plasma membrane, i

201 e targeted either toward the reformation and budding of synaptic vesicles, toward secretion via exocy

202 oscopy and super-resolution imaging show the budding of syntaphilin cargos, which then share a ride o

203 Budding of TACV Z was Tsg101 independent but required th

204 BBLF1 is hypothesized to facilitate the budding of tegumented capsid into glycoprotein-embedded

205 Budding of the (nucleo)capsid at and scission from the i

206 Osmotically induced budding of the ATPS-containing GVs led to structures whe

207 However, the budding of the chimeric virus was delayed and infectious

208 ived vesicles (ectosomes), formed by outward budding of the ciliary membrane, carry enzymes and other

209 genitor cells is impaired due to inefficient budding of the cysts and a failure of the cells in the c

210 ates that ILVs form individually from inward budding of the endosomal limiting membrane, plant ILVs f

211 Subsequent inward budding of the endosomal membrane generates multivesicul

212 ing of ubiquitinated cargo with intralumenal budding of the endosomal membrane, two essential steps i

213 During budding of the epithelial tubes of the salivary glands i

214 e and neomycin, but not brefeldin A, inhibit budding of the ESVs in vitro.

215 The pharyngeal pouches, which form by budding of the foregut endoderm, are essential for segme

216 ubiquitinated transmembrane proteins and the budding of the HIV virus.

217 t-negative (DN) mutant of WWP1 could inhibit budding of the intact HTLV-1 virus.

218 f is generally insufficient to induce robust budding of the isolated WD in culture.

219 examined the role of the MVB pathway in the budding of the late-domain-containing rhabdovirus vesicu

220 Heintzelman et al. suggested that budding of the limbs is caused by a higher liquid-like c

221 FGFR1 signaling results in increased lateral budding of the mammary ductal epithelium, and that susta

222 Here we focus on its ability to promote budding of the mature virus from the cell surface.

223 s to have direct biological relevance during budding of the nascent influenza virus, which does not u

224 nsitivity to RET signaling, including excess budding of the ND, increased phospho-ERK and increased e

225 pUL34, and misregulated, capsid-independent budding of the NE.

226 To investigate the requirements for budding of the paramyxovirus simian virus 5 (SV5), its M

227 (CME) involves nanoscale bending and inward budding of the plasma membrane, by which cells regulate

228 to 600-nm microvesicles derived from direct budding of the plasma membrane, while the second pool is

229 Vs), a main type of EVs generated by outward budding of the plasma membrane.

230 Native cytosol requires ATP to initiate the budding of the pre-chylomicron transport vesicle from in

231 hat inhibited the budding of SFV but not the budding of the rhabdovirus vesicular stomatitis virus.

232 intra-endoplasmic reticulum (ER) sorting and budding of the RING-domain peroxins (Pex2, Pex10, and Pe

233 ical for the intra-ER sorting and subsequent budding of the RING-domain peroxins.

234 ules is determined stochastically during the budding of the somatic organelles from the trans-Golgi n

235 The budding of the urogenital sinus epithelium into the surr

236 vity is required for both maintenance of the budding of the venous endothelial cells and differentiat

237 ruses of several other families, entails the budding of the viral nucleocapsid through the plasma mem

238 40 (mVP40), which regulates the assembly and budding of the virions.

239 ne indentations rich in PI(4,5)P2 and inward budding of these membrane domains into the lumen of GUVs

240 Annexin VI is not required for the budding of these new coated pits and ALLN does not inhib

241 Budding of these viruses is dependent on the presence of

242 critical role for late domain motifs in the budding of these viruses, including Ebola virus, it rema

243 Budding of this chimera was severely impaired, however,

244 cells do not appear to be necessary for the budding of this organ.

245 o interact with a ubiquitin (Ub) ligase, and budding of this virus is dependent on Ub.

246 les, and mutation of this sequence inhibited budding of transfected M2 protein in vivo.

247 The budding of transport vesicles from the Golgi complex is

248 act with EH domain proteins that function in budding of transport vesicles from the plasma membrane o

249 inker upstream of the zipper domain leads to budding of tubular rather than spherical particles.

250 ng, as the anchors that induced the greatest budding of TyA-GFP are the same as those that mediate re

251 osphate-binding domain induced only a slight budding of TyA-GFP, approximately 2% of control, and no

252 Gag lacks PPxY motifs, we now show that the budding of various HIV-1 L-domain mutants is dramaticall

253 are believed to support the transport and/or budding of vesicles along microtubules, were tested.

254 transport, but not with those that block the budding of vesicles from the ER.

255 atidylethanolamine asymmetry and the dynamic budding of vesicles from the plasma membrane, supporting

256 This step might involve budding of vesicles from the trans-Golgi.

257 e, and gamma-synergin, which may mediate the budding of vesicles in the trans-Golgi complex.

258 B vesicles but is not a prerequisite for the budding of vesicles into the endosome lumen.

259 d pits on the plasma membrane and subsequent budding of vesicles is an energetically demanding proces

260 ARF regulates the budding of vesicles that mediate endoplasmic reticulum t

261 or GGAs) are minimal components required for budding of vesicles.

262 In the secretory pathway, budding of vesicular transport carriers from the trans-G

263 ruses are complex processes that require the budding of viral nucleocapsids into the lumen of cytopla

264 that is activated during, or shortly after, budding of viral particles from the surface of infected

265 d is characterized by polarized assembly and budding of virions and clustering of cellular organelles

266 lymphocytes resulted in a massive and rapid budding of virions from lymphocytes, followed by their i

267 nfectious virus into the culture medium, and budding of virions from the plasma membrane.

268 ix protein (eVP40) orchestrates assembly and budding of virions in part by hijacking select WW-domain

269 rted to the plasma membrane for assembly and budding of virions.

270 gent that interferes with cell migration and budding of virus from lipid rafts, blocks transmission o

271 owth by impairing late steps in the assembly/budding of virus particles at the plasma membrane.

272 This promotes budding of virus particles into cytoplasmic vesicles der

273 ate phase of infection for a short duration, budding of virus particles, as determined by protein ana

274 interaction also appears to be important for budding of virus particles.

275 Hrs222-777 can recruit Tsg101 and rescue the budding of virus-like Gag particles that are missing nat

276 owed that the M protein alone can induce the budding of virus-like particles (vesicles) from the plas

277 s the JUNV Z-Tsg101 interaction and inhibits budding of virus-like particles (VLPs) driven by ectopic

278 iphoton fluorescence microscopy to visualize budding of virus-like particles (VLPs) of Rous sarcoma v

279 mammalian cells results in the formation and budding of virus-like particles (VLPs) which mimic the b

280 III filaments, we examine HIV-1 Gag-mediated budding of virus-like particles and find that depleting

281 e of PR release from Gag, also do not affect budding of virus-like particles from cells.

282 s required late in replication for efficient budding of virus-like particles from cells.

283 xpression of a single protein, Gag, leads to budding of virus-like particles into the extracellular s

284 The only retrovirus protein required for the budding of virus-like particles is the Gag protein; howe

285 rvation may reflect either rapid Z-dependent budding of virus-like particles upon coassociation or a

286 hat formation of this dimer is essential for budding of virus-like particles.

287 loss of RSV filament formation and a lack of budding of virus-like particles.

288 rus polyprotein Gag co-opts this process for budding of virus-like particles.

289 s show that proteasome inhibitors reduce the budding of viruses that utilize either a PPPY- or PTAP-b

290 ne fission event topologically resembles the budding of viruses, such as HIV-1, from infected cells.

291 sorting (VPS) machinery, is required for the budding of viruses, such as human immunodeficiency virus

292 us sarcoma virus Gag abolishes formation and budding of VLPs at the plasma membranes of baculovirus-i

293 e of the arenavirus-encoded proteins rescued budding of VLPs in the presence of BST-2.

294 14-3-3 protein overexpression reduced the budding of VLPs.

295 f VP40 which subsequently leads to efficient budding of VLPs.

296 The assembly and budding of VP40 from the plasma membrane of host cells s

297 e "x" is any amino acid) that facilitate the budding of VP40-containing VLPs.

298 PTAP and PPEY motifs contribute to efficient budding of VP40-containing VLPs; (ii) PTAP and PPEY can

299 Budding of WAEVs requires secretory carrier-associated m

300 rts a strong dominant-negative effect on the budding of wild-type Gag, further supporting the importa