ライフサイエンスコーパス: butyryl

1 Furthermore, butyryl ACP itself underwent slow conformational exchang

2 rate-bound form of ACP (crystal structure of butyryl-ACP from Escherichia coli) suggests that conform

3 Unexpectedly, butyryl act ACP showed almost identical backbone (1)H-(1

4 iled analyses, which indicated that (1) only butyryl-acyl carrier protein (ACP) and S-adenosylmethion

5 hway is based on a thioesterase specific for butyryl-acyl carrier protein (ACP), which allows native

6 tonization but did induce the deacylation of butyryl-acyl carrier protein.

7 yl-ADP-ribose, O-propionyl-ADP-ribose, and O-butyryl-ADP-ribose to produce ADP-ribose (ADPr) and acet

8 ost potent (EC(50): 1 nM), followed by the N-butyryl analogue.

9 oxidation cycle with optimal activity toward butyryl- and hexanoyl-CoA.

10 nhibitor (IC50 = 30 nM), 1-(2-amino-3-methyl-butyryl)-azetidine-2-carbonitrile (AMAC), which has show

11 similar analog, 1-(2-dimethylamino-3-methyl-butyryl)-azetidine-2-carbonitrile (DAMAC), that does not

12 talyzed the transfer of acetyl-, propionyl-, butyryl-, benzoyl-, phenylacetyl-, and malonylphosphopan

13 tion of T(alpha) peptides carrying maleimido-butyryl-biocytin by avidin-agarose chromatography; and (

14 N-maleimide moiety of the reagent, maleimido-butyryl-biocytin, containing a biotinyl group; (iv) tryp

15 4, 6, or 7 conferred both acetyl (AChE) and butyryl (BuChE) cholinesterase inhibitory activities at

16 ale mice oxidizing myristoyl-, octanoyl-, or butyryl-carnitine as well.

17 cGMP and its analogs, 8-Br-cGMP and 2'-butyryl-cGMP, also competed with the Pgamma-1-83BC C ter

18 ppropriate acid chlorides (benzoyl chloride, butyryl chloride, and ethyl chlorooxoacetate).

19 ion of enzyme, acetyl cholinesterase (AChE), butyryl cholinesterase (BChE), tyrosinase and alkaline p

20 viously shown by proton NMR that horse serum butyryl cholinesterase, like serine proteases, forms a s

21 as monoamine oxidases (MAOs) and acetyl- and butyryl-cholinesterase (AChE and BChE) inhibitors.

22 Acetyl- and butyryl-cholinesterase (AChE and BuChE) activities were

23 In contrast, human or equine butyryl-cholinesterase (BuChE) converted CPT-11 to SN-38

24 iomarkers to the active sites of acetyl- and butyryl-cholinesterase enzymes revealed promising bindin

25 e seen in mRNA levels of the related enzyme, butyryl-cholinesterase, nor of the high-affinity choline

26 e, acetoacetyl coenzyme A (acetoacetyl-CoA), butyryl CoA, acetoacetate, and beta-hydroxybutyrate.

27 talyze the condensation of malonyl-pfACP and butyryl-CoA (k(cat) 200 min(-1), K(M) 35.7 +/- 4.4 micro

28 imple equation: crotonyl-CoA + NADH + H(+) = butyryl-CoA + NAD(+) with Km = 1.4 mum ferredoxin or 2.0

29 Priming DEBS with endogenous butyryl-CoA affords an alternative and more direct route

30 the interconversion of isobutyryl-CoA and n-butyryl-CoA also catalyzes the interconversion between i

31 ible interconversion of isobutyryl-CoA and n-butyryl-CoA and exists as a heterotetramer.

32 nthesis in S. collinus, which suggested that butyryl-CoA and isobutyryl-CoA function as starter units

33 Butyryl-CoA and isobutyryl-CoA interacted with the acety

34 The DEBS polyketide synthase (PKS) used butyryl-CoA and methylmalonyl-CoA supplied in vivo by th

35 oA dehydrogenase has maximal activity toward butyryl-CoA and negligible activity toward substrates lo

36 ylate via a novel pathway thought to involve butyryl-CoA and propionyl-CoA as intermediates.

37 We have shown that we can produce butyryl-CoA at levels of up to 50% of the total CoA pool

38 tical to those seen for MM-CoA; in addition, butyryl-CoA binds and behaves in a manner similar to pro

39 e biosynthesis and that either acetyl-CoA or butyryl-CoA can be a starter unit for palmitate biosynth

40 ssigned to bcd(red):crotonyl-CoA and bcd(ox):butyryl-CoA charge-transfer complexes, demonstrating the

41 The final reduction of crotonyl-CoA to butyryl-CoA completes the cycle, which we call the semiq

42 substrates of ZhuH, although acetyl-CoA and butyryl-CoA could also be accepted and elongated.

43 dies, the biosynthesis of the palmitate from butyryl-CoA decreases in the presence of thiolactomycin.

44 and rapid-reaction kinetics of the isolated butyryl-CoA dehydrogenase (bcd) component of the electro

45 Electron-transferring flavoprotein (Etf) and butyryl-CoA dehydrogenase (Bcd) from Acidaminococcus fer

46 A (CoA) dehydrogenase (BHBD), crotonase, and butyryl-CoA dehydrogenase (BCD) from Clostridium acetobu

47 s genes for a D-lactate dehydrogenase (Ldh), butyryl-CoA dehydrogenase (Bcd), and LrgAB.

48 a-FADH(-)) transfers one electron further to butyryl-CoA dehydrogenase (Bcd); two such transfers enab

49 a-FADH(-)) transfers one electron further to butyryl-CoA dehydrogenase (Bcd); two such transfers enab

50 with alpha-FAD and beta-FAD) and tetrameric butyryl-CoA dehydrogenase (Bcd, with delta-FAD in each s

51 ectron transferring flavoprotein (EtfAf) and butyryl-CoA dehydrogenase (BcdAf) of Acidaminococcus fer

52 in decreased abundance of the gene encoding butyryl-coA dehydrogenase (P=0.02).

53 ween an acetyl-, propionyl-, isobutyryl-, or butyryl-CoA derived primer unit and a malonyl-CoA derive

54 at CCR plays a significant role in providing butyryl-CoA for monensin A biosynthesis and is present i

55 reductase, which converts acetoacetyl-CoA to butyryl-CoA for use as a 4C extender unit during tylacto

56 tylicum are responsible for the formation of butyryl-CoA from acetoacetyl-CoA.

57 n high concentrations of 4-hydroxy-4-phospho-butyryl-CoA in brain and liver.

58 catalyzes the conversion of crotonyl-CoA to butyryl-CoA in the presence of NADPH, was previously clo

59 EBS 1+TE can convert acetyl-, propionyl-, or butyryl-CoA into the corresponding C8-, C9-, and C10-lac

60 particular, its tolerance toward acetyl- and butyryl-CoA is unexpectedly strong.

61 he high concentration of 4-hydroxy-4-phospho-butyryl-CoA may be related to the cerebral dysfunction o

62 e inactivation upon incubation with either n-butyryl-CoA or isobutyryl-CoA.

63 tase, a primary metabolic enzyme involved in butyryl-CoA production in streptomycetes, was expressed

64 lysines correlated with the acetyl-CoA: (iso)butyryl-CoA ratio in liver.

65 ed as a transcriptional unit and form a BCS (butyryl-CoA synthesis) operon.

66 The butyryl-CoA synthesized was further extended to hexanoyl

67 mutant using butyryl-, crotonyl-, and 2-aza-butyryl-CoA thioesters.

68 nd R207Q) to catalyze the rearrangement of n-butyryl-CoA to isobutyryl-CoA.

69 e the exergonic reduction of crotonyl-CoA to butyryl-CoA to the endergonic reduction of ferredoxin bo

70 lmitate (a branched-chain fatty acid), while butyryl-CoA was converted to palmitate (a straight-chain

71 in 824(pAADB1) fermentations suggested that butyryl-CoA was limiting butanol production in 824(pAADB

72 ed with GroEL/ES, and the R147W variant when butyryl-CoA was used as a substrate.

73 inactivation when either isobutyryl-CoA or n-butyryl-CoA was used as substrate.

74 f the loading didomain, although acetyl- and butyryl-CoA were also accepted with approximately 40-fol

75 t was unable to exchange the alpha-proton of butyryl-CoA with D(2)O.

76 ucial metabolic intermediates acetyl-CoA and butyryl-CoA with substantial velocities.

77 Optimal substrate (butyryl-CoA) was seen to shift the flavin redox potentia

78 action (lactate + crotonyl-CoA -> pyruvate + butyryl-CoA) yielded a k(cat) of 2.5 +/- 0.1 s(-1) and a

79 9-h lag in growth was observed, during which butyryl-CoA, a degradation product of ethylmalonyl-CoA,

80 f 2.4 microM for acetyl-CoA, 0.71 microM for butyryl-CoA, and 0.41 microM for isobutyryl-CoA.

81 yme A (CoA) derivatives, such as acetyl-CoA, butyryl-CoA, HMG-CoA, and malonyl-CoA, as well as NADPH

82 substrate specificity and is able to accept butyryl-CoA, leading to the production of polyketides wi

83 bcd with crotonyl-CoA and oxidized bcd with butyryl-CoA, long-wavelength-absorbing intermediates are

84 th the results using our standard substrates butyryl-CoA, octanoyl-CoA, and palmitoyl-CoA.

85 04 muM and 5.2 +/- 0.5 muM for D-lactate and butyryl-CoA, respectively.

86 the interconversion of isobutyryl-CoA and n-butyryl-CoA, whereas GTPase activity is associated with

87 ensation of two acetyl-CoA molecules to form butyryl-CoA, which is then transformed to succinyl-CoA w

88 h the priming of the enzyme by acetyl-CoA or butyryl-CoA.

89 ng acetyl-CoA approximately propionyl-CoA >> butyryl-CoA.

90 to Bcd for the reduction of crotonyl-CoA to butyryl-CoA.

91 ansfers enable Bcd to reduce crotonyl-CoA to butyryl-CoA.

92 n which is distinct from the metabolism of n-butyryl-CoA.

93 ansfers enable Bcd to reduce crotonyl-CoA to butyryl-CoA.

94 and Dh-FADH(-) that converts crotonyl-CoA to butyryl-CoA.

95 skeleton rearrangement of isobutyryl-CoA to butyryl-CoA.

96 enzyme caused the midpoint potential for the butyryl-CoA/crotonyl-CoA couple (E(BCoA/CCoA)) to shift

97 en measured in the presence and absence of a butyryl-CoA/crotonyl-CoA mixture.

98 Butyryl-CoA:acetate CoA-transferase activity was detecte

99 ere analyzed for 16S rRNA sequencing and the butyryl-CoA:acetateCoA transferase (BCoAT) gene expressi

100 M), which interconverts isobutyryl-CoA and n-butyryl-CoA; ethylmalonyl-CoA mutase, which interconvert

101 Most of these genes were related to butyryl coenzyme A and butyrate formation and/or assimil

102 butyrate formation genes (ptb and buk); the butyryl coenzyme A biosynthesis operon genes; fructose b

103 s of the butyrate-producing bacterial enzyme butyryl-coenzyme A (CoA):acetate CoA-transferase (BCoAT)

104 ate kinase and phosphotransbutyrylase or via butyryl-coenzyme A (CoA):acetate CoA-transferase.

105 the relative abundance of the gene encoding butyryl-coenzyme A (CoA):acetate-CoA-transferase, a majo

106 hen present at physiological concentrations, butyryl-coenzyme A and NADPH were not substrates for Rhl

107 of wild-type and the Glu367-Gln mutant using butyryl-, crotonyl-, and 2-aza-butyryl-CoA thioesters.

108 n from both cell types, as are forskolin, di-butyryl cyclic adenosine monophosphate, and adrenocortic

109 e of an acylated species of ACP is that of a butyryl derivative of Escherichia coli ACP.

110 f 7-deacetyl-7-(O-[N-methylpiperazino]-gamma-butyryl)-dihydrochloride-forskolin (MPB-forskolin), we f

111 nd 7-deacetyl-7-O-(N-methylpiperazino)-gamma-butyryl-forskolin was effective exclusively in the PFH,

112 n of 7beta-deacetyl-7beta-[gamma-(morpholino)butyryl]-forskolin hydrochloride (7Db-forskolin, a water

113 in when provided with a soluble beta-hydroxy-butyryl-heptapeptidyl thioester substrate.

114 Act ACP was also derivatised with butyryl, hexanoyl, and octanoyl groups.

115 ombinant wild type SCAD kcat/K(m) values for butyryl-hexanoyl-, and octanoyl-CoA were 220, 22, and 3.

116 (m) of 9.3, 2.8, and 1.5 microM-1 min-1 with butyryl-, hexanoyl-, and octanoyl-CoA used as substrates

117 inosa, along with its cognate autoinducer, N-butyryl homoserine lactone (C(4)-HSL), regulates gene ex

118 ne (3OC12-HSL) (formerly called PAI-1) and N-butyryl homoserine lactone (C4-HSL) (formerly called PAI

119 decanoyl)-homoserine lactone (C12-HSL) and N-butyryl homoserine lactone (C4-HSL), on cell viability a

120 production by a large excess of exogenous N-butyryl homoserine lactone (C4-HSL).

121 , and RhlI, which directs the synthesis of N-butyryl homoserine lactone (PAI-2).

122 Increased amounts of rhamnolipids and N-butyryl homoserine lactone were detected in the biofilm

123 system is composed of RhlR and the signal N-butyryl homoserine lactone.

124 N-(3-oxododecanoyl) homoserine lactone and N-butyryl homoserine lactone].

125 red for growth, addition of the RhlI product butyryl-homoserine lactone (C4-HSL), or bacteria that pr

126 sI mutant PAO-MW1 alongside plasma treated N-butyryl-homoserine lactone and n-(3-oxo-dodecanoyl)-homo

127 , N-3-oxododecanoyl-homoserine lactone and N-butyryl-homoserine lactone, can both enter eukaryotic ce

128 ), whereas RhlI catalyzes the synthesis of N-butyryl-homoserine lactone.

129 2,3,6-Tri-O-butyryl-Ins(1,4,5)P(3)/AM (6-Ins(1,4,5)P(3)), a cell-per

130 l (k(cat)/K(m) 15.8 +/- 1.8 m(-1) s(-1)) and butyryl (k(cat)/K(m) 17.5 +/- 2.1 m(-1) s(-1)) derivativ

131 d exsC expression, resulting in increased N-(butyryl)-l-homoserine-lactone quorum sensing signal and

132 l-homoserine lactone (3-oxo-C(12)-HSL) and N-butyryl-l-homoserine lactone (C(4)-HSL), respectively.

133 (3-oxododecanoyl)-L-homoserine lactone and N-butyryl-L-homoserine lactone (C(4)-HSL), which are known

134 en coupled to FabI, purified P. aeruginosa N-butyryl-L-homoserine lactone (C4-HSL) synthase, RhlI, co

135 ; (4) RhlI was able to direct synthesis of N-butyryl-L-homoserine lactone from crotonyl-ACP in a reac

136 decanoyl)-L-homoserine lactone) and PAI-2 (N-butyryl-L-homoserine lactone) respectively.

137 N-(3-oxododecanoyl)-L-homoserine lactone, N-butyryl-L-homoserine lactone, and the Pseudomonas quinol

138 (3-oxododecanoyl)-L-homoserine lactone and N-butyryl-L-homoserine lactone.

139 convert authentic N-butyrylvinylglycine to N-butyryl-L-homoserine lactone.

140 heir cognate autoinducer ligand and not by N-butyryl-L-homoserine lactone.

141 onine (SAM) were required for synthesis of N-butyryl-L-homoserine lactone; (2) when present at physio

142 resulting odourless and tasteless prodrug (O-butyryl-L-serine, which we named SerBut) from the gut, e

143 developed that contain a genetically encoded butyryl lysine and are subsequently used to select for l

144 Interestingly, propionyl- and butyryl-lysine peptides were found to bind tighter to Hs

145 Sirt2, and Sirt3, suggesting propionyl- and butyryl-lysine proteins may be sirtuin substrates in viv

146 quinone chromophore in the dyad using an iso-butyryl mask.

147 d hyaluronan, the total NH2, N-acetyl, and N-butyryl moieties were 0, 82.2 +/- 4.6, and 22.7 +/- 3.8%

148 gly, NDTBT transferred hexanoyl, acetyl, and butyryl more rapidly than butenoyl or benzoyl from the C

149 ll permeant and non-radiolabeled 2,5,6-tri-O-butyryl-myo-inositol 1,3, 4-trisphosphate-hexakis(acetox

150 NANS module 2+TE with (+/-)-2-methyl-3-keto-butyryl-N-acetylcysteamine thioester (1), the SNAC analo

151 ine was found to be negligible and that of n-butyryl-pantetheinephosphate low, and therefore, it is e

152 The assay relies on 2'-amino-butyryl-pyrene-uridine incorporated in a 58-nucleotide r

153 A single intermediate, assigned to N-butyryl- S-adenosylmethionine, was observed.

154 the carboxylate oxygen of the presumptive N-butyryl-SAM intermediate attacks the methylene carbon ad