ライフサイエンスコーパス: butyryl-CoA

1 and Dh-FADH(-) that converts crotonyl-CoA to butyryl-CoA.

2 ansfers enable Bcd to reduce crotonyl-CoA to butyryl-CoA.

3 skeleton rearrangement of isobutyryl-CoA to butyryl-CoA.

4 h the priming of the enzyme by acetyl-CoA or butyryl-CoA.

5 ng acetyl-CoA approximately propionyl-CoA >> butyryl-CoA.

6 to Bcd for the reduction of crotonyl-CoA to butyryl-CoA.

7 ansfers enable Bcd to reduce crotonyl-CoA to butyryl-CoA.

8 n which is distinct from the metabolism of n-butyryl-CoA.

9 9-h lag in growth was observed, during which butyryl-CoA, a degradation product of ethylmalonyl-CoA,

10 Butyryl-CoA:acetate CoA-transferase activity was detecte

11 ere analyzed for 16S rRNA sequencing and the butyryl-CoA:acetateCoA transferase (BCoAT) gene expressi

12 e, acetoacetyl coenzyme A (acetoacetyl-CoA), butyryl CoA, acetoacetate, and beta-hydroxybutyrate.

13 Priming DEBS with endogenous butyryl-CoA affords an alternative and more direct route

14 the interconversion of isobutyryl-CoA and n-butyryl-CoA also catalyzes the interconversion between i

15 ible interconversion of isobutyryl-CoA and n-butyryl-CoA and exists as a heterotetramer.

16 nthesis in S. collinus, which suggested that butyryl-CoA and isobutyryl-CoA function as starter units

17 Butyryl-CoA and isobutyryl-CoA interacted with the acety

18 The DEBS polyketide synthase (PKS) used butyryl-CoA and methylmalonyl-CoA supplied in vivo by th

19 oA dehydrogenase has maximal activity toward butyryl-CoA and negligible activity toward substrates lo

20 ylate via a novel pathway thought to involve butyryl-CoA and propionyl-CoA as intermediates.

21 f 2.4 microM for acetyl-CoA, 0.71 microM for butyryl-CoA, and 0.41 microM for isobutyryl-CoA.

22 We have shown that we can produce butyryl-CoA at levels of up to 50% of the total CoA pool

23 tical to those seen for MM-CoA; in addition, butyryl-CoA binds and behaves in a manner similar to pro

24 e biosynthesis and that either acetyl-CoA or butyryl-CoA can be a starter unit for palmitate biosynth

25 ssigned to bcd(red):crotonyl-CoA and bcd(ox):butyryl-CoA charge-transfer complexes, demonstrating the

26 The final reduction of crotonyl-CoA to butyryl-CoA completes the cycle, which we call the semiq

27 substrates of ZhuH, although acetyl-CoA and butyryl-CoA could also be accepted and elongated.

28 enzyme caused the midpoint potential for the butyryl-CoA/crotonyl-CoA couple (E(BCoA/CCoA)) to shift

29 en measured in the presence and absence of a butyryl-CoA/crotonyl-CoA mixture.

30 dies, the biosynthesis of the palmitate from butyryl-CoA decreases in the presence of thiolactomycin.

31 and rapid-reaction kinetics of the isolated butyryl-CoA dehydrogenase (bcd) component of the electro

32 Electron-transferring flavoprotein (Etf) and butyryl-CoA dehydrogenase (Bcd) from Acidaminococcus fer

33 A (CoA) dehydrogenase (BHBD), crotonase, and butyryl-CoA dehydrogenase (BCD) from Clostridium acetobu

34 s genes for a D-lactate dehydrogenase (Ldh), butyryl-CoA dehydrogenase (Bcd), and LrgAB.

35 a-FADH(-)) transfers one electron further to butyryl-CoA dehydrogenase (Bcd); two such transfers enab

36 a-FADH(-)) transfers one electron further to butyryl-CoA dehydrogenase (Bcd); two such transfers enab

37 with alpha-FAD and beta-FAD) and tetrameric butyryl-CoA dehydrogenase (Bcd, with delta-FAD in each s

38 ectron transferring flavoprotein (EtfAf) and butyryl-CoA dehydrogenase (BcdAf) of Acidaminococcus fer

39 in decreased abundance of the gene encoding butyryl-coA dehydrogenase (P=0.02).

40 ween an acetyl-, propionyl-, isobutyryl-, or butyryl-CoA derived primer unit and a malonyl-CoA derive

41 M), which interconverts isobutyryl-CoA and n-butyryl-CoA; ethylmalonyl-CoA mutase, which interconvert

42 at CCR plays a significant role in providing butyryl-CoA for monensin A biosynthesis and is present i

43 reductase, which converts acetoacetyl-CoA to butyryl-CoA for use as a 4C extender unit during tylacto

44 tylicum are responsible for the formation of butyryl-CoA from acetoacetyl-CoA.

45 yme A (CoA) derivatives, such as acetyl-CoA, butyryl-CoA, HMG-CoA, and malonyl-CoA, as well as NADPH

46 n high concentrations of 4-hydroxy-4-phospho-butyryl-CoA in brain and liver.

47 catalyzes the conversion of crotonyl-CoA to butyryl-CoA in the presence of NADPH, was previously clo

48 EBS 1+TE can convert acetyl-, propionyl-, or butyryl-CoA into the corresponding C8-, C9-, and C10-lac

49 particular, its tolerance toward acetyl- and butyryl-CoA is unexpectedly strong.

50 talyze the condensation of malonyl-pfACP and butyryl-CoA (k(cat) 200 min(-1), K(M) 35.7 +/- 4.4 micro

51 substrate specificity and is able to accept butyryl-CoA, leading to the production of polyketides wi

52 bcd with crotonyl-CoA and oxidized bcd with butyryl-CoA, long-wavelength-absorbing intermediates are

53 he high concentration of 4-hydroxy-4-phospho-butyryl-CoA may be related to the cerebral dysfunction o

54 imple equation: crotonyl-CoA + NADH + H(+) = butyryl-CoA + NAD(+) with Km = 1.4 mum ferredoxin or 2.0

55 th the results using our standard substrates butyryl-CoA, octanoyl-CoA, and palmitoyl-CoA.

56 e inactivation upon incubation with either n-butyryl-CoA or isobutyryl-CoA.

57 tase, a primary metabolic enzyme involved in butyryl-CoA production in streptomycetes, was expressed

58 lysines correlated with the acetyl-CoA: (iso)butyryl-CoA ratio in liver.

59 04 muM and 5.2 +/- 0.5 muM for D-lactate and butyryl-CoA, respectively.

60 ed as a transcriptional unit and form a BCS (butyryl-CoA synthesis) operon.

61 The butyryl-CoA synthesized was further extended to hexanoyl

62 mutant using butyryl-, crotonyl-, and 2-aza-butyryl-CoA thioesters.

63 nd R207Q) to catalyze the rearrangement of n-butyryl-CoA to isobutyryl-CoA.

64 e the exergonic reduction of crotonyl-CoA to butyryl-CoA to the endergonic reduction of ferredoxin bo

65 lmitate (a branched-chain fatty acid), while butyryl-CoA was converted to palmitate (a straight-chain

66 in 824(pAADB1) fermentations suggested that butyryl-CoA was limiting butanol production in 824(pAADB

67 ed with GroEL/ES, and the R147W variant when butyryl-CoA was used as a substrate.

68 inactivation when either isobutyryl-CoA or n-butyryl-CoA was used as substrate.

69 Optimal substrate (butyryl-CoA) was seen to shift the flavin redox potentia

70 f the loading didomain, although acetyl- and butyryl-CoA were also accepted with approximately 40-fol

71 the interconversion of isobutyryl-CoA and n-butyryl-CoA, whereas GTPase activity is associated with

72 ensation of two acetyl-CoA molecules to form butyryl-CoA, which is then transformed to succinyl-CoA w

73 t was unable to exchange the alpha-proton of butyryl-CoA with D(2)O.

74 ucial metabolic intermediates acetyl-CoA and butyryl-CoA with substantial velocities.

75 action (lactate + crotonyl-CoA -> pyruvate + butyryl-CoA) yielded a k(cat) of 2.5 +/- 0.1 s(-1) and a