ライフサイエンスコーパス: c-di-GMP

1 c-di-GMP can assume alternative oligomeric states to eff

2 c-di-GMP caused a persistent increase in cAMP, which sti

3 c-di-GMP interaction leads to active site obstruction, h

4 c-di-GMP interacts with a conserved N-terminal RxxxR mot

5 c-di-GMP interacts with PilZ C-domain motifs 1 and 2 (Rx

6 c-di-GMP often regulates the function of its protein tar

7 c-di-GMP signals are integrated into the genetic differe

8 c-di-GMP, a universal bacterial second messenger, can tr

9 CFP) as a biomass indicator and the GFP as a c-di-GMP reporter.

10 For this, we developed a c-di-GMP-sequestering peptide (CSP) that was derived fro

11 rmation and dispersal is mediated by LapD, a c-di-GMP receptor, and LapG, a periplasmic protease, whi

12 tional and biochemical characterization of a c-di-GMP PDE, PdcA, 1 of 37 confirmed or putative c-di-G

13 ylate (c-di-GMP), by increased activity of a c-di-GMP specific phosphodiesterase.

14 econd messenger, 3-5 cyclic diguanylic acid (c-di-GMP) to the master repressor, BldD.

15 activated by a 3',5'-cyclic diguanylic acid (c-di-GMP)-regulated transcription factor, MrkH.

16 Additionally, c-di-GMP was found to be localized at the outer boundary

17 Although c-di-GMP is known to stimulate the innate sensor STING,

18 also compare the mechanisms of c-di-AMP and c-di-GMP binding by the respective receptors that allow

19 Comparison of apo- and c-di-GMP-bound MrkH structures reveals a large 138 degre

20 onmental regulation of biofilm formation and c-di-GMP signalling.

21 necting role between cellular metabolism and c-di-GMP signalling in P. putida.

22 inally, we directly detect elevated pGpG and c-di-GMP in the orn strain.

23 nfirm coupling of the ATPase active site and c-di-GMP binding, as well as the functional significance

24 e regions in apo structure stabilization and c-di-GMP interaction allows distinction between the stat

25 d c-di-GMP-induced cAMP synthesis as well as c-di-GMP-induced stalk gene transcription.

26 of the biofilm and thus represent an average c-di-GMP concentration across the entire biofilm.

27 suggest that, as opposed to other bacteria, c-di-GMP turns down the T6SS in A. tumefaciens thus impa

28 ystal structure of a ternary complex between c-di-GMP, sigma(WhiG), and its anti-sigma factor, RsiG.

29 The interaction between c-di-GMP and the ATPase MshE promotes pilus extension, w

30 d by c-di-GMP, thus revealing a link between c-di-GMP signaling and chromosome biology.

31 irulence factors, and suggest a link between c-di-GMP signaling and nutrient availability.

32 -95 alters the binding stoichiometry between c-di-GMP and Alg44 from 2:1 to 1:1.

33 us, as revealed by a telling synergy between c-di-GMP and DIF-1.

34 outcome without undesired cross talk between c-di-GMP-dependent systems.

35 us formation were shown to specifically bind c-di-GMP.

36 naling pathway, FleQ, has been shown to bind c-di-GMP.

37 CSP binds c-di-GMP with submicromolar affinity.

38 RsiG binds c-di-GMP in the absence of sigma(WhiG), employing a nove

39 We also show that SadB binds c-di-GMP with higher affinity than FleQ and propose that

40 of the ribbon-helix-helix family that binds c-di-GMP in Myxococcus xanthus.

41 BldD-(c-di-GMP) sits on top of the regulatory network that con

42 tion necessitates the assembly of the BldD2-(c-di-GMP)4 complex.

43 influence the cell cycle by influencing both c-di-GMP and (p)ppGpp.

44 of coincidence detection that relies on both c-di-GMP and LapG binding to LapD for receptor activatio

45 for c-di-GMP binding abolish binding to both c-di-GMP and DNA, rendering these protein variants non-f

46 one component of the protection afforded by c-di-GMP.

47 ation of the ML beta-glucan synthase BgsA by c-di-GMP binding to its C-terminal domain.

48 suggest that the regulation of chemotaxis by c-di-GMP through MapZ orthologs/homologs is widespread i

49 BldD activity is controlled by c-di-GMP concentration and BldO potentially responds to

50 , and its activity is directly controlled by c-di-GMP.

51 nthesize c-di-GMP, whereas it is degraded by c-di-GMP-specific phosphodiesterases (PDEs).

52 estalk cells reduced stalk gene induction by c-di-GMP, whereas PKA activation bypassed the c-di-GMP r

53 est that the protective response mediated by c-di-GMP is multifactorial, involving chemotactic respon

54 chromosome organization and is modulated by c-di-GMP, thus revealing a link between c-di-GMP signali

55 e factors have been shown to be regulated by c-di-GMP.

56 Indeed, domain reorientation by c-di-GMP complexation with MrkH, which leads to a highly

57 In these cases, c-di-GMP-induced cell death was rescued by complementati

58 ly by restoring BrlR production and cellular c-di-GMP levels to wild-type levels.

59 ction cascade leading to changes in cellular c-di-GMP levels remains unknown, certain L- and D-amino

60 omponent signaling system, increase cellular c-di-GMP levels, and signal the onset of the cell cycle.

61 e biosynthesis genes in response to cellular c-di-GMP.

62 t GGDEF-EAL domain arrangement, RmdA cleaves c-di-GMP to GMP and exhibits residual DGC activity.

63 rulence, and confirm the existence of common c-di-GMP signalling pathways that are capable of regulat

64 However, at physiological concentrations c-di-GMP is a monomer and little is known about how high

65 Many bacteria contain c-di-GMP-metabolizing enzymes but lack known c-di-GMP re

66 In C. crescentus, c-di-GMP works as a major regulator of pole morphogenesi

67 The elucidation of the CSP.c-di-GMP complex structure by NMR identified a linear c-

68 dictions, that SpoT can effectively decrease c-di-GMP levels in response to nitrogen starvation just

69 related proteins that can contain degenerate c-di-GMP turnover domains.

70 RmcA activity such that the protein degrades c-di-GMP and thereby inhibits matrix production during o

71 terium Azoarcus sp. strain CIB that degrades c-di-GMP in response to aromatic hydrocarbons, including

72 In addition, a CfcR-dependent c-di-GMP boost was observed during this stage in Deltars

73 2 (RxxxR and D/NxSxxG) and a newly described c-di-GMP-binding motif in the MrkH N domain.

74 Hence P. aeruginosa is able to differentiate c-di-GMP output using structurally highly related protei

75 e intestinal pathogen Clostridium difficile, c-di-GMP inhibits flagellar motility and toxin productio

76 on is regulated by 3',5'-cyclic diguanylate (c-di-GMP) and requires production of the type IV mannose

77 The second messenger cyclic diguanylate (c-di-GMP) controls diverse cellular processes among bact

78 Cyclic diguanylate (c-di-GMP) is a broadly conserved intracellular second me

79 Cyclic diguanylate (c-di-GMP) is a near universal signaling molecule produce

80 n artificial increase of cyclic diguanylate (c-di-GMP) levels in Sinorhizobium meliloti 8530, a bacte

81 The signaling molecule cyclic diguanylate (c-di-GMP) mediates physiological adaptation to extracell

82 cterial second messenger cyclic diguanylate (c-di-GMP) regulates a wide range of cellular functions f

83 bility landscapes of the cyclic diguanylate (c-di-GMP) riboswitch.

84 of the second messenger, cyclic diguanylate (c-di-GMP), by increased activity of a c-di-GMP specific

85 previously reported that cyclic diguanylate (c-di-GMP), synthesized by diguanylate cyclase A (DgcA),

86 the signalling molecule, cyclic diguanylate (c-di-GMP).

87 the residues required for binding of dimeric c-di-GMP in vitro are also required for efficient algina

88 Bacterial usage of the cyclic dinucleotide c-di-GMP is widespread, governing the transition between

89 ketide DIF-1 or by the cyclical dinucleotide c-di-GMP.

90 nvolve signaling by the cyclic dinucleotides c-di-GMP and c-di-AMP.

91 y mechanisms of gene expression under direct c-di-GMP control via FleQ and FleQ-like bEBPs.

92 NG-dependent host response to cytosolic DNA, c-di-GMP, cGAMP, HIV-1, and DNA viruses.

93 gulatory mechanisms governing control of EAL c-di-GMP phosphodiesterases.

94 Thus, the CFP/GFP ratio gives the effective c-di-GMP per biomass.

95 an EPS biosynthesis gene cluster at elevated c-di-GMP levels.

96 n structure: while DeltarbdA showed elevated c-di-GMP levels, restricted motility and promoted biofil

97 a-anti-sigma complex formation and establish c-di-GMP as the central integrator of Streptomyces devel

98 dA phosphodiesterase mutant producing excess c-di-GMP displays marked attenuation in vitro and in viv

99 sses conserved motifs with high affinity for c-di-GMP binding, the findings here suggest that c-di-GM

100 icating that removal of pGpG is critical for c-di-GMP homeostasis.

101 A main effector for c-di-GMP signaling in the opportunistic pathogen Pseudom

102 gca- structures to identify target genes for c-di-GMP, and used these genes to investigate the c-di-G

103 substitutions in CdbA regions important for c-di-GMP binding abolish binding to both c-di-GMP and DN

104 ating that cAMP is also the intermediate for c-di-GMP in vivo.

105 ng a prototypical transmembrane receptor for c-di-GMP, LapD, and a cognate periplasmic protease, LapG

106 next to pssZ, are primarily responsible for c-di-GMP-dependent EPS production.

107 mmary, our results indicate a vital role for c-di-GMP in allowing Brucella to successfully navigate s

108 This is an additional role for c-di-GMP in bacterial physiology.

109 ted motility and promoted biofilm formation, c-di-GMP levels were decreased in Deltapa2072, and biofi

110 Using flow cells for biofilm formation, c-di-GMP showed a non-uniform distribution across the bi

111 R, which is responsible for most of the free c-di-GMP during stationary phase in static conditions.

112 Global c-di-GMP levels were unaffected by spermine supplementat

113 ed by variations in local rather than global c-di-GMP pools.

114 he bacterial second messenger cyclic di-GMP (c-di-GMP) controls biofilm formation and other phenotype

115 The second messenger cyclic di-GMP (c-di-GMP) controls the transition between different life

116 hat the protein levels of two cyclic di-GMP (c-di-GMP) diguanylate cyclases (DGCs), GcpA and GcpL, ar

117 he bacterial second messenger cyclic di-GMP (c-di-GMP) has emerged as a prominent mediator of bacteri

118 Cyclic di-GMP (c-di-GMP) is a second messenger that modulates multiple

119 Cyclic di-GMP (c-di-GMP) is a widespread second messenger that plays a

120 racellular signaling molecule cyclic di-GMP (c-di-GMP) regulates the lifestyle of bacteria and contro

121 The cyclic di-GMP (c-di-GMP) second messenger represents a signaling system

122 sition requires activation of cyclic di-GMP (c-di-GMP) synthesis by the Hk1/Rrp1 TCS; B. burgdorferi

123 prokaryotic second messenger cyclic di-GMP (c-di-GMP) to coordinate responses to shifting environmen

124 tion via the second messenger cyclic di-GMP (c-di-GMP).

125 of second messengers such as cyclic di-GMP (c-di-GMP).

126 Cyclic dimeric GMP (c-di-GMP) has emerged as a key regulatory player in the

127 ular secondary messenger cyclic dimeric-GMP (c-di-GMP) in response to environmental conditions.

128 The complex metabolic pathways governing c-di-GMP synthesis and degradation are highly regulated,

129 els of bis-(3',5')-cyclic-dimeric-guanosine (c-di-GMP), a second messenger that stimulates matrix pro

130 lates the biofilm mode of life, and a higher c-di-GMP concentration reduces cell detachment from biof

131 rovide detailed structural insights into how c-di-GMP controls the activity of an enzyme target indir

132 elae is a monofunctional PDE that hydrolyzes c-di-GMP to 5'pGpG.

133 regulated, but the specific cues that impact c-di-GMP signaling are largely unknown.

134 selected on the basis of predicted impaired c-di-GMP turnover function: DeltafimX showed increased,

135 lm formation by P. putida through changes in c-di-GMP content and altered expression of structural el

136 on: adaptation though incremental changes in c-di-GMP network proteins acquires knowledge from past e

137 ginine pools appears to determine changes in c-di-GMP turnover.

138 h hydrolyze a single phosphodiester group in c-di-GMP to produce 5'-phosphoguanylyl-(3',5')-guanosine

139 of many genes encoding enzymes implicated in c-di-GMP turnover.

140 ibit a low-temperature-dependent increase in c-di-GMP, indicating that these DGCs are required for te

141 e main one causing a significant increase in c-di-GMP.

142 , including a large set of genes involved in c-di-GMP biosynthesis, degradation, and transmission.

143 e response regulator domain that resulted in c-di-GMP degradation.

144 orn strain could inhibit PDE-As, increasing c-di-GMP concentration.

145 have been ascribed to any of the individual c-di-GMP synthases or phosphodiesterases (PDEs).

146 affect DGC activity of SadC, OdaI inhibited c-di-GMP production by SadC.

147 conformation of the riboswitch that inhibits c-di-GMP binding.

148 n that is induced by binding an intercalated c-di-GMP dimer.

149 revealing an unusual, partially intercalated c-di-GMP dimer bound at the RsiG-sigma(WhiG) interface.

150 44 in complex with dimeric self-intercalated c-di-GMP and characterize its dinucleotide-binding site

151 -binding motif, in which a self-intercalated c-di-GMP dimer is tightly bound by a network of H bonds

152 Here, we present a strategy to intercept c-di-GMP signaling pathways by directly targeting the se

153 e that endogenously expressed CSP intercepts c-di-GMP signaling and effectively inhibits biofilm form

154 ng swimming motility or global intracellular c-di-GMP.

155 inosa Deltaorn mutant had high intracellular c-di-GMP levels, causing this strain to overexpress extr

156 ndent PDEs, thereby increasing intracellular c-di-GMP in Deltaorn cells.

157 e world-the input stimuli-into intracellular c-di-GMP levels that then regulate genes for biofilm for

158 cer, which cues degradation of intracellular c-di-GMP leading to transcription of the swarming progra

159 nd fluorescence was calibrated against known c-di-GMP concentrations.

160 c-di-GMP-metabolizing enzymes but lack known c-di-GMP receptors.

161 P are associated with the biofilm lifestyle, c-di-GMP hydrolysing phosphodiesterases (PDEs) have been

162 tide (CSP) that was derived from a CheY-like c-di-GMP effector protein.

163 complex structure by NMR identified a linear c-di-GMP-binding motif, in which a self-intercalated c-d

164 erases (PDE-As) end signaling by linearizing c-di-GMP to 5'-phosphoguanylyl-(3',5')-guanosine (pGpG),

165 sterically regulated by GTP, further linking c-di-GMP levels to nutrient availability.

166 re identified as critical to maintaining low c-di-GMP concentrations generated after initial phagocyt

167 Maintenance of low c-di-GMP concentrations by these phosphodiesterases was

168 ation phase and biofilm formation, while low c-di-GMP levels unleash T6SS and T4SS to advance plant c

169 ponse than did wild-type Brucella or the low-c-di-GMP guanylate cyclase DeltacgsB mutant.

170 Our data suggest that the major c-di-GMP-controlled targets determining the timing and m

171 generate a fluorescent biosensor to measure c-di-GMP concentrations in thousands of individual bacte

172 The second messenger c-di-GMP (or cyclic diguanylate) regulates biofilm forma

173 Elevated levels of the second messenger c-di-GMP activate biosynthesis of an unknown exopolysacc

174 response to binding of the second messenger c-di-GMP to a C-terminal extension.

175 ncrease in the level of the second messenger c-di-GMP.

176 trated by the intracellular second-messenger c-di-GMP.

177 ecule, the intracellular secondary messenger c-di-GMP (Bis-(3'-5')-cyclic dimeric guanosine monophosp

178 Second messengers, c-di-GMP and (p)ppGpp, are vital regulatory molecules in

179 cribe the role of two key second messengers, c-di-GMP and cAMP, in this process.

180 AmrZ itself has been shown to modulate c-di-GMP levels through the control of many genes encodi

181 -amino acids have been described to modulate c-di-GMP turnover in some bacteria.

182 We also show that temperature modulates c-di-GMP levels in a similar fashion in the Gram-negativ

183 reviously showed that the signaling molecule c-di-GMP binds BldD, a master repressor, to control init

184 Cyclic diguanosine monophosphate (c-di-GMP) is a second messenger that controls diverse fu

185 is-(3'-5') cyclic diguanosine monophosphate (c-di-GMP) phosphodiesterase MbaA.

186 RNA) RsmB, cyclic diguanylate monophosphate (c-di-GMP) and flagellar regulator have been reported to

187 regulator cyclic diguanylate monophosphate (c-di-GMP) through changes in the activity and localizati

188 -5')-cyclic-dimeric-guanosine monophosphate (c-di-GMP) acts as an innate immune system modulator.

189 ,5')-cyclic dimeric guanosine monophosphate (c-di-GMP) binding activity post-translationally regulate

190 nger cyclic dimeric guanosine monophosphate (c-di-GMP) by posttranscriptionally repressing expression

191 r bis-3,5-cyclic di-guanosine monophosphate (c-di-GMP) determines when Streptomyces initiate sporulat

192 -5')-cyclic dimeric guanosine monophosphate (c-di-GMP) in cells respiring on nitrate than those grown

193 -5')-cyclic dimeric guanosine monophosphate (c-di-GMP) is a dynamic intracellular signaling molecule

194 ger cyclic di-3',5'-guanosine monophosphate (c-di-GMP) is a key regulator of bacterial motility and v

195 d by cyclic dimeric guanosine monophosphate (c-di-GMP).

196 -5')-cyclic dimeric guanosine monophosphate (c-di-GMP).

197 le PDE gene is sufficient to impact multiple c-di-GMP-dependent phenotypes, including the production

198 ed and synthesized by simplifying the native c-di-GMP structure and replacing the charged phosphodies

199 affinity cyclic GMP-AMP (3'3'-cGAMP) but not c-di-GMP or 2'3'-cGAMP.

200 In the absence of c-di-GMP, ShkA predominantly adopts a compact domain arr

201 This work also revealed a basal affinity of c-di-GMP-unbound receptor for LapG, the relevance of whi

202 AdVCA0848) that produces elevated amounts of c-di-GMP when expressed in mammalian cells in vivo.

203 of free CheR1, revealed that the binding of c-di-GMP induces dramatic structural changes in MapZ tha

204 We identified a class of c-di-GMP-responsive proteins, represented by the AraC-li

205 review provides an up-to-date compendium of c-di-GMP pathways connected to biofilm formation, biofil

206 to catalyze the synthesis and degradation of c-di-GMP.

207 ose a mathematical model for the dynamics of c-di-GMP and (p)ppGpp in C. crescentus and analyze how t

208 ide insights into the molecular evolution of c-di-GMP binding to proteins.

209 ese results suggest that the dimeric form of c-di-GMP represents the biologically active signaling mo

210 be endogenous, as shown by the inability of c-di-GMP to induce cell death in Dictyostelium HMX44A ce

211 aintain high viability and a higher level of c-di-GMP to reduce cell detachment.

212 mutants, instead having an elevated level of c-di-GMP, suggesting that the role of Bd1971 is to moder

213 As high levels of c-di-GMP are associated with the biofilm lifestyle, c-di

214 motes pilus extension, whereas low levels of c-di-GMP correlate with enhanced retraction.

215 We also demonstrate that elevated levels of c-di-GMP within the cell decrease the activity of the Ty

216 conditions by modulating cellular levels of c-di-GMP.

217 action, excess pGpG extends the half-life of c-di-GMP, indicating that removal of pGpG is critical fo

218 Although unrelated in sequence, the mode of c-di-GMP binding to CuxR is highly reminiscent to that o

219 ional data that reveal an unexpected mode of c-di-GMP recognition that is associated with major confo

220 Here, we analyzed the binding mode of c-di-GMP to the allosteric inhibitory site (I-site) of d

221 ines the proposed global and local models of c-di-GMP signaling specificity in bacteria, and attempts

222 s are required for temperature modulation of c-di-GMP levels.

223 n is also controlled by a complex network of c-di-GMP-metabolizing enzymes.

224 urium virulence was due to overproduction of c-di-GMP-regulated cellulose, as deletion of the cellulo

225 adenovirus vaccine, fostering production of c-di-GMP as well as proinflammatory responses in mice.

226 Expression profiling of c-di-GMP-regulated genes through the enzootic cycle supp

227 for the in situ, real time quantification of c-di-GMP and show that the amount of this biofilm-regula

228 rent methodologies for the quantification of c-di-GMP are typically based on chemical extraction, rep

229 We found that pGpG reduced the rate of c-di-GMP degradation in cell lysates and inhibited the a

230 To determine the role of c-di-GMP in Brucella physiology and in shaping host-Bruc

231 the biofilm, with concentrated hot spots of c-di-GMP.

232 As revealed by the structure of c-di-GMP-complexed FleQ, the second messenger interacts

233 these data confirm that in vivo synthesis of c-di-GMP stimulates strong innate immune responses that

234 de (FMN) variant class, and also variants of c-di-GMP-I and -II riboswitches that might recognize dif

235 e analysed the influence of L-amino acids on c-di-GMP levels in the plant-beneficial bacterium Pseudo

236 RsmA also showed a negative impact on c-di-GMP levels in a double mutant DeltarsmIE through th

237 There is limited information on c-di-GMP metabolism, particularly on regulatory mechanis

238 opose that such peculiar control reflects on c-di-GMP being a key second messenger that silences ener

239 ow the PTS (Ntr) system influences (p)ppGpp, c-di-GMP, GMP and GTP concentrations.

240 l, our data support a role for the predicted c-di-GMP-binding protein LapD in inhibiting LapG-depende

241 ant at the restrictive temperature prevented c-di-GMP-induced cAMP synthesis as well as c-di-GMP-indu

242 d1971 interacts with several GGDEF proteins (c-di-GMP producers), but mutants of Bd1971 do not share

243 a beta-barrel domain, represents a prototype c-di-GMP receptor.

244 phenotype from proteins containing putative c-di-GMP turnover and Per-Arnt-Sim (PAS) sensory domains

245 GMP PDE, PdcA, 1 of 37 confirmed or putative c-di-GMP metabolism proteins in C. difficile 630.

246 e highly conserved residues markedly reduces c-di-GMP binding and biofilm formation by V. cholerae.

247 5-mutagenesis shows that M6 killing requires c-di-GMP-dependent signalling, diverse fungicides and re

248 We present the structure of the RsiG-(c-di-GMP)(2)-sigma(WhiG) complex, revealing an unusual,

249 d regulatory network that connects the sRNA, c-di-GMP signalling and flagellar master regulator FlhDC

250 Diguanylate cyclases synthesize c-di-GMP, whereas it is degraded by c-di-GMP-specific ph

251 s are tested for their capacity of targeting c-di-GMP signaling.

252 response regulator domain, and a C-terminal c-di-GMP phosphodiesterase (PDE) domain.

253 Here we demonstrate that c-di-GMP also intervenes later in development to control

254 Further studies demonstrate that c-di-GMP is essential for RsiG to inhibit sigma(WhiG).

255 en together, these results demonstrated that c-di-GMP could trigger cell death in Dictyostelium only

256 (2020) demonstrates that c-di-GMP controls spore formation in Streptomyces venezu

257 We show here that c-di-GMP was not sufficient to induce cell death in Dict

258 Our results indicate that c-di-GMP directly controls MshE activity, thus regulatin

259 These data also raise the possibility that c-di-GMP enhances the expression of a subset of RpoS-dep

260 h higher affinity than FleQ and propose that c-di-GMP produced by AdrA modulates flagella synthesis t

261 Proteomics analysis revealed that c-di-GMP regulates several processes critical for virule

262 Here, we show that c-di-GMP binds BldD using an ordered, sequential mechani

263 -GMP binding, the findings here suggest that c-di-GMP can regulate both motility and biofilm formatio

264 The c-di-GMP network has a bow-tie shaped architecture that

265 Additionally, the c-di-GMP-governed auto-aggregation and biofilm phenotype

266 ylate cyclases (DGCs) CdgB and CdgC, and the c-di-GMP phosphodiesterases (PDEs) RmdA and RmdB, are po

267 to residues in the C-domain motif 2 and the c-di-GMP-binding N-domain motif.

268 releasing inhibition of protease LapG by the c-di-GMP effector protein LapD, and resulting in proteol

269 -di-GMP, whereas PKA activation bypassed the c-di-GMP requirement for stalk gene expression.

270 we observed increased transcription from the c-di-GMP-regulated pel promoter.

271 rimetric binding analysis of residues in the c-di-GMP binding site demonstrate that mutation of Arg-1

272 necessary to complete the final step in the c-di-GMP degradation pathway.

273 GMP, and used these genes to investigate the c-di-GMP signal transduction pathway.

274 Moreover, expression of the c-di-GMP and calcium-regulated, biofilm-promoting brp ex

275 I)(L/V/I)xxxxLxxxLxxQ that binds half of the c-di-GMP molecule, primarily through hydrophobic interac

276 cence protein (GFP) under the control of the c-di-GMP-responsive cdrA promoter (Rybtke, M.

277 cence protein (GFP) under the control of the c-di-GMP-responsive cdrA promoter.

278 The structure shows that the c-di-GMP binding region of Alg44 adopts a PilZ domain fo

279 mdA, and DeltarmdB strains revealed that the c-di-GMP specified by these enzymes has a global regulat

280 ts we propose a mathematical model where the c-di-GMP network is analogous to a machine learning clas

281 In their c-di-GMP bound conformation Cle proteins interact with t

282 Strikingly, these c-di-GMP-binding motifs also stabilize an open state con

283 This c-di-GMP-binding motif is present in diverse bacterial p

284 Thus, c-di-GMP cannot only stabilize domain interactions, but

285 ntrol to this cell death and perhaps also to c-di-GMP effects in other situations and organisms.

286 late receptor function and may also apply to c-di-GMP-metabolizing enzymes that are akin to LapD.

287 The 3D structure of MotI bound to c-di-GMP was solved, and MotI-fluorescent fusions locali

288 ons and that this activity is fundamental to c-di-GMP signal transduction.

289 nd to define the sensor enzymes important to c-di-GMP regulation.

290 ational changes in the receptor that lead to c-di-GMP-dependent protease recruitment.

291 utida biofilms, nutrient starvation triggers c-di-GMP hydrolysis by phosphodiesterase BifA, releasing

292 ifies the elusive function of the ubiquitous c-di-GMP network, a key regulator of bacterial social tr

293 This structure reveals a unique c-di-GMP-binding mode, featuring a tandem array of two h

294 e and mechanism of a previously unrecognized c-di-GMP-responsive transcription factor and provide ins

295 ping host-Brucella interactions, we utilized c-di-GMP regulatory enzyme deletion mutants.

296 While c-di-GMP and (p)ppGpp are both synthesized from GTP mole

297 ity of the signaling network associated with c-di-GMP in P. aeruginosa.

298 The interactions of CdbA with c-di-GMP and DNA appear to be mutually exclusive and res

299 protein MapZ cocrystallized in complex with c-di-GMP and its protein target CheR1, a chemotaxis-regu

300 N1-145) from Vibrio cholerae in complex with c-di-GMP at a 1.37 A resolution.