ライフサイエンスコーパス: cDNA clone

1 subsequently incorporated into a full-length cDNA clone.

2 ome lineage expressed from a D. melanogaster cDNA clone.

3 d with in vitro transcripts of an infectious cDNA clone.

4 alanines by mutagenesis of an infectious FCV cDNA clone.

5 t background of a recombinant VSV infectious cDNA clone.

6 t RNA synthesis pattern was recovered from a cDNA clone.

7 with a (32)P-labeled probe derived from the cDNA clone.

8 to the LC coding region of an infectious FCV cDNA clone.

9 un cloned into a modified 1a/JFH1 infectious cDNA clone.

10 l VEEV strain originating from an infectious cDNA clone.

11 collections contain principally two types of cDNA clones.

12 al of 68,721 ESTs were generated from 68,131 cDNA clones.

13 5' sequences from about half that number of cDNA clones.

14 oncoding transcript represented by two IMAGE cDNA clones.

15 and derived 5' ESTs from >30,000 unique rat cDNA clones.

16 enetics system to recover UUKV entirely from cDNA clones.

17 as proposed from the analysis of overlapping cDNA clones.

18 rtially sequenced over 5100 random T. reesei cDNA clones.

19 ymerase in all eight independently generated cDNA clones.

20 overed from the sequence of rhabdomyosarcoma cDNA clones.

21 t sequencing or pyrosequencing of individual cDNA clones.

22 re obtained from random sequencing of 16,152 cDNA clones.

23 ae by multiple chromatographic steps, led to cDNA cloning.

24 transcription-polymerase chain reaction and cDNA cloning.

25 ikely an artifactual sequence created during cDNA cloning.

26 By use of an infectious SAT2 cDNA clone, 10 structurally exposed and highly variable

27 From 6316 cDNA clones, 3980 retinal expressed sequence tags (ESTs)

28 A second type of cDNA clone, a 'full-ORF' (F-ORF) expression clone, is on

29 The availability of these infectious cDNA clones affords us an opportunity to understand the

30 By combining cDNA cloning, Affymetrix (Santa Clara, CA) genechips cov

31 ese strategies--sequencing randomly selected cDNA clones, aligning protein sequences identified in ot

32 irect delivery in mice of an infectious ZIKV cDNA clone allows the rescue of recombinant (r)ZIKV in v

33 By using a "pure" culture of CTV from a cDNA clone and green fluorescent protein-labeled virus w

34 mutagenized p7 of an infectious genotype 1a cDNA clone and tested RNA transcripts of each mutant for

35 MaxT permutation analysis using t tests (20 cDNA clones and 10 unique genes), significance analysis

36 ), significance analysis for microarrays (33 cDNA clones and 15 genes, at an estimated false discover

37 For example, the value of full-length cDNA clones and deep expressed sequence tag resources, o

38 port the generation of infectious SFTSV from cDNA clones and demonstrate that the behavior of recombi

39 this system is effective for both individual cDNA clones and for cDNA libraries, permitting the direc

40 erformed a gain-of-function screen of 15,779 cDNA clones and identified 40 genes affecting exon 18 of

41 se that will aid creation of functional NPHV cDNA clones and other novel tools for hepacivirus studie

42 sion in lymphocytes determined by sequencing cDNA clones and quantifying 6FAM-labeled PCR products fo

43 Analysis of cDNA clones and RT-PCR with total mRNA revealed alternat

44 Thus, obtaining full-length cDNA clones and sequences for most or all genes in an or

45 sing differential RNA selection, full-length cDNA cloning and 454 transcriptome sequencing of the ric

46 This observation has led to the cDNA cloning and characterization of a fourth SCD isofor

47 This study describes cDNA cloning and characterization of mouse RALDH4.

48 un-based approach that led ultimately to the cDNA cloning and functional characterization of many of

49 diterpene synthase sequences for full-length cDNA cloning and functional characterization.

50 ed the expression of four of the isoforms by cDNA cloning and mass spectrometric analysis of proteins

51 fragments may have arisen in the process of cDNA cloning and not from splicing abnormalities.

52 cDNA cloning and RNA sequencing (RNA-Seq) were used to i

53 B), 22 (BCL8C), 2 (BCL8D), and 10 (BCL8E) by cDNA cloning and sequence analysis.

54 cDNA cloning and sequencing has shown that allurin is a

55 Using a library of cDNAs (1.8 x 10(6) cDNA clones) and an intermittent cisplatin selection sys

56 utated to alanine using a type A full-genome cDNA clone, and the virus progeny was analyzed for defec

57 d myosin as determined using immunoblotting, cDNA cloning, and/or LC-MS/MS.

58 chniques; functionally related sets of human cDNA clones; and genome-scale gene collections for Sacch

59 cDNA clones are valuable reagents for functional studies

60 Libraries of cDNA clones are valuable resources for analyzing the exp

61 Using a 9,280-cDNA clone array, we have compared steady-state RNA from

62 Therefore, we constructed chimeric cDNA clones between CHIKV and VEEV or SFV to probe the e

63 ibe the construction of the first human PARG cDNA clone by reverse transcription of CF3 human fibrobl

64 Open reading frames (ORFs) derived from cDNA clones can be used to generate constructs allowing

65 Using cDNA cloning, cell culture expression, and activity assa

66 SOURCE provides content both in gene and cDNA clone-centric pages, and thus simplifies analysis o

67 We have identified a putative SWI3-like cDNA clone, CHB2 (AtSWI3B), from Arabidopsis thaliana by

68 ription-PCR and assembled into a full-length cDNA clone, clone C, which contained 14 mutations compar

69 IgE-reactive cDNA clones coding for portions of high molecular weight

70 Initial sequence analysis of our murine cDNA clone collections showed that as much as 86, 45, an

71 Subsequent studies showed that the human cDNA clone complemented an ATR-deficient bacterial mutan

72 The cDNA clone comprised an open reading frame of 1461 bp en

73 and JHM.WU and, utilizing these full-length cDNA clones, constructed chimeric viruses and mapped the

74 titutional effort to identify and sequence a cDNA clone containing a complete ORF for each human and

75 The infectivity of an RUB infectious cDNA clone containing the mutations D1210A/D1217A was de

76 WV, we designed a series of novel infectious cDNA clones corresponding to coexisting DWV genotypes, t

77 solated and characterized various myotrophin cDNA clones corresponding to the multiple transcripts by

78 reveals homologies to a previously reported cDNA clone, CP8.

79 titution of G1100D in an infectious DA virus cDNA clone demonstrated a major role for this mutation i

80 Hybridomas and cDNA clones derived from the immunized mice included man

81 The cDNA clone-derived RNA is infectious in cells, generatin

82 mutation introduced into an infectious DENV2 cDNA clone did not yield detectable virus by plaque assa

83 We identified a tomato cDNA clone, DIG3, that encodes a protein that interacts

84 created by transfection of a genotype I HDV cDNA clone directly into the liver of a woodchuck that w

85 ill summarize the major collections of human cDNA clones, discuss some limitations common to most of

86 rging from large-scale genome sequencing and cDNA cloning efforts.

87 with the EP4 receptor as a bait identified a cDNA clone encoding a 669-amino acid protein, designated

88 cles could be rescued from a full-length FCV cDNA clone encoding a nonfunctional VP2 when VP2 was pro

89 A cDNA clone encoding a presumed full-length glycine-rich

90 In this study, a cDNA clone encoding a putative ACC oxidase, PtACO1, was

91 uences identified a single full-length maize cDNA clone encoding all the peptide sequences obtained f

92 A cDNA clone encoding alpha-conotoxin GI, the first conoto

93 at a single intraperitoneal inoculation of a cDNA clone encoding an attenuated rZIKV was safe, highly

94 ing the methods of proteomics, a full-length cDNA clone encoding an enzyme matching the properties of

95 A full-length cDNA clone encoding hebraein was isolated from a cDNA li

96 s of a zebrafish cDNA library, we isolated a cDNA clone encoding receptor activity-modifying protein

97 A cDNA clone encoding the entire 14,150-nucleotide genome

98 omplementary DNA (cDNA) library to isolate a cDNA clone encoding the ferritin heavy chain polypeptide

99 A cDNA clone encoding the human B cell alloantigen DC alph

100 A cDNA clone encoding the lignin-related enzyme caffeoyl C

101 Analysis of a cDNA clone encoding the prepropeptide precursor of pl14a

102 genomes were constructed from two infectious cDNA clones encoding a virulent and an attenuated isolat

103 interaction of CB3-RD with DAF, we produced cDNA clones encoding both CB3-RD and CB3-Nancy and mutat

104 on and sequencing of genomic and full-length cDNA clones encoding DC3.

105 We isolated several related but distinct cDNA clones encoding novel structure proteins (NSP) when

106 right Yellow-2 protoplasts identified single cDNA clones encoding proteins that interact with either,

107 equence tag database, we have identified two cDNA clones encoding putative cytosolic STs.

108 trap in yeast cells was utilized to identify cDNA clones encoding putative secreted proteins.

109 Twenty-three different cDNA clones encoding putative toxins were characterized

110 the complementary DNA (cDNA) for eNOS and 23 cDNA clones encoding the Asp-His-His-Cys motif (DHHC) pa

111 cDNA clones encoding the enzyme that synthesizes CGA, hy

112 Infectious cDNA clones encoding the marker viruses also contain uni

113 Analysis of cDNA clones encoding the novel peptides as well as those

114 of 19-kD alpha-zein genes, we characterized cDNA clones encoding these proteins from a developing en

115 t here the isolation of a complementary DNA (cDNA) clone encoding one such enzyme, mannan synthase (M

116 erminus of exon 7 in all M. nemestrina TRIM5 cDNA clones examined.

117 ent HDA+SSCP patterns were detected among 33 cDNA clones examined.

118 nify analogous doubly spliced Rej mRNAs, and cDNA clones expressing two of them also showed Rej activ

119 We describe herein the cDNA cloning, expression, and characterization of a hemo

120 We have identified this factor as the novel cDNA clone FL14676485 that encodes for the human hypothe

121 We identified a cDNA clone for epiprofin, which is preferentially expres

122 , we report the development of an infectious cDNA clone for the SVA/HLJ/CHA/2016 strain.

123 be used at any scale, from the isolation of cDNA clones for a particular gene of interest, to the im

124 The isolation of full-length cDNA clones for each of these candidate genes resulted i

125 We isolated two corresponding cDNA clones for Sfhex that encode proteins with >99% ami

126 teins led to the identification of a 4.75-kb cDNA clone from a Strongylocentrotus purpuratus ovary cD

127 A cDNA clone from human brain encoding the mammalian homol

128 We previously sequenced an R2 cDNA clone from the yellow fever mosquito, Aedes aegypti

129 Here we demonstrate that the CRSBP-1 cDNA cloned from bovine liver libraries encodes a 322-re

130 ea mays, including ESTs representing 484,032 cDNA clones from 53 libraries and 36,565 fully sequenced

131 By screening of 1500 cDNA clones from a resistant-susceptible mosquito subtra

132 , rhinophores), we isolated five full-length cDNA clones from an A. californica central nervous syste

133 cDNA microarrays with >11,000 cDNA clones from an NOD spleen cDNA library were used to

134 ecules, MHC class I genes were identified in cDNA clones from Arabian horse A2152, which presented bo

135 striction enzymes, nor the sequencing of 130 cDNA clones from benign and malignant breast tissue and

136 Approximately 2x10(6) cDNA clones from each genotype were sequenced, correspon

137 ions against a microarray consisting of 2173 cDNA clones from five pine expressed sequence tag librar

138 echniques, we measured mRNA levels in 11 283 cDNA clones from the cerebral cortex of Tg2576 mice and

139 and searching and sequencing the NF2-related cDNA clones from the IMAGE consortium.

140 We aligned full-length cDNA clones from the Mammalian Gene Collection to the hu

141 Differential display was used to isolate cDNA clones from the three species showing differential

142 cDNA clones from the UGT2A3 gene (located on chromosome

143 A total of 11,000 cDNA clones from these libraries, representing 5,866 uni

144 b genes, Ssxb1 to Ssxb12, were identified by cDNA cloning from mouse testis and mouse tumors.

145 I), and salmon GnRH (sGnRH) were isolated by cDNA cloning from the brain of the Atlantic croaker, Mic

146 The slc5a8 cDNA, cloned from mouse kidney, codes for a protein cons

147 gene encoding Cl.ir channels in heart, ClC-2 cDNAs cloned from rat (rClC-2) and guinea pig (gpClC-2)

148 ions were found in CD247 complementary DNAs (cDNAs) cloned from the patient as well as in cDNA and ge

149 mutations at M-58 and E-191 in the chimeric cDNA clones further improved the viability of the chimer

150 Forty-eight of 89 (54%) sequenced cDNA clones had large deletions, each beginning and/or e

151 cDNA clones harboring viral sequences were selected and

152 cDNA clones have long been valuable reagents for studyin

153 the coding region of genes, and full-length cDNA clones have proven to be useful for investigation o

154 plication of a second virus generated from a cDNA clone, HRV-C11.

155 etermine the function of CBP, an orthologous cDNA clone (Hs CBP) was isolated from the sugar beet cys

156 f the 2'-O-MTase using a virulent infectious cDNA clone, icPC22A, as the backbone.

157 Random sequencing of cDNA clones identified approximately 100 unique species.

158 cDNA cloning identified a 2733-bp transcript from AT6.1

159 Extensive cDNA cloning identified long and short forms of Xtrb2, t

160 m a single human fetal fast skeletal TnTbeta cDNA clone in order to circumvent the problem of N-termi

161 tions were introduced into an infectious FCV cDNA clone in order to evaluate the functional importanc

162 We characterized nine cDNA clones in details.

163 nes that were not represented by full-length cDNA clones in our Drosophila Gene Collection.

164 Additionally, at least 55% of the cDNA clones in this study were completely or partially a

165 CHV1-EP713 infectious cDNA clones in which the p48 coding region was deleted,

166 umber alterations across 42,000 mapped human cDNA clones, in a series of 54 gliomas of varying histog

167 Sequence analysis of these cDNA clones indicates that each clone has a unique 5' UT

168 Here, we isolated a cDNA clone Ipeglu1 encoding Ipecac alkaloid beta-D-gluco

169 The full-length zebrafish CTCF cDNA clone is 4244 bp in length with an open reading fra

170 This cDNA clone is available at the American Type Culture Col

171 cDNA cloning is a central technology in molecular biolog

172 We constructed infectious cDNA clones lacking the ER retrieval signal, the endocyt

173 A cDNA clone library of the expressed 16S rRNA corroborate

174 Some but not all of these cDNA clones may represent the entire mRNA sequence, but

175 nomic hybridization (CGH) analysis of a 4559 cDNA clone microarray.

176 After next-generation RNA sequencing, viral cDNA clones mimicking the enterovirus RNA sequences foun

177 We report the full-length cDNA cloning, molecular characterization and functional

178 Two DFR cDNA clones (MtDFR1 and MtDFR2) were isolated from the m

179 In vitro transcripts from an infectious cDNA clone mutated to eliminate potential glycosylation

180 A highly reactive cDNA clone of 1,428 bp encoded a trichomonad protein of

181 A cDNA clone of 1.1 kb encoding a 108-aa polypeptide was i

182 An infectious cDNA clone of a genotype 3 strain of hepatitis E virus a

183 We isolated a full-length cDNA clone of amphioxus AmphiNk2-tin, an NK2 gene simila

184 bovine virulence, we assembled an infectious cDNA clone of an RGD-containing A(24)Cru and derived mut

185 y site-directed mutagenesis on a full-length cDNA clone of BC.

186 th open reading frame (ORF) of an infectious cDNA clone of HCV (genotype 1a, H77 strain) in the nontr

187 An infectious cDNA clone of hepatitis E virus was mutated in order to

188 The 2861 bp cDNA clone of hGC-1 contained an open reading frame of 1

189 truction and the properties of a full-length cDNA clone of HRV16, pR16.11, which produces in vitro tr

190 Here, an infectious cDNA clone of IBV was used to address the importance of

191 ced, polyadenylated BART RNAs, a full-length cDNA clone of one of the BART isoforms was obtained and

192 We used a full-length cDNA clone of ONNV to construct a series of mutants in w

193 ogical disease, we constructed a full-length cDNA clone of silver-haired bat-associated RV (SHBRV) st

194 In this study, we constructed a full-length cDNA clone of SL-CoV WIV1 (rWIV1), an ORFX deletion muta

195 constructs engineered based on an infectious cDNA clone of T36 isolate of CTV, including hybrids cont

196 s in JPV, a plasmid containing a full-length cDNA clone of the genome of JPV was constructed.

197 We inserted a full-length cDNA clone of the genomic RNA of the dicistrovirus Rhopa

198 nt wild-type 88-1961 strain (r88) and from a cDNA clone of the highly attenuated Jeryl Lynn vaccine s

199 aring the 197-aa deletion from an infectious cDNA clone of the highly virulent PEDV PC22A strain (inf

200 A partial cDNA clone of the mouse orthologue was obtained by RT-PC

201 ting of combinations of genes derived from a cDNA clone of the neurovirulent wild-type 88-1961 strain

202 A cDNA clone of unknown function, DEA1, was isolated from

203 e these mutants, we engineered an infectious cDNA clone of VSV and employed N-RNA templates from thos

204 g sequences were inserted into a full-length cDNA clone of VSV, and the virus recovery, kinetics of g

205 We first constructed a stable full-length cDNA clone of ZIKV in a novel linear vector from which i

206 We report an infectious cDNA clone of ZIKV that was generated using a clinical i

207 th IFN sensitivity and virulence, infectious cDNA clones of a virulent North American strain and the

208 Three of the seven cDNA clones of centromeric genes from rice centromere 8

209 lowing inoculation with RNA transcripts from cDNA clones of hepatitis C virus (HCV).

210 transiently in HEK293 cells using expression cDNA clones of human alpha1C, alpha2delta, and beta subu

211 We have used recombinant cDNA clones of representative persistent (CR6) and nonpe

212 and in vivo characterizations of infectious cDNA clones of swine HEV.

213 In this study, cDNA clones of the cotton KCBP homolog GhKCBP were isola

214 ectious virus was recovered from each of the cDNA clones of the HN glycoprotein mutants, employing a

215 tagged oda5 allele, we isolated genomic and cDNA clones of the wild-type gene.

216 Since the development of infectious cDNA clones of viral RNA genomes and the means of delive

217 t on the purification, characterization, and cDNA cloning of a novel UGAT involved in the biosynthesi

218 northern bobwhite myoglobin were deduced by cDNA cloning of the coding sequence from mRNA.

219 from 53 libraries and 36,565 fully sequenced cDNA clones, out of which 31,552 clones are non-redundan

220 A full-length cDNA clone predicts a novel vertebrate-specific protein

221 es rapid subtraction hybridization (RaSH) of cDNA clones prepared from two cell populations, a driver

222 orresponding to exon 10, which is missing in cDNAs cloned previously from human tissues.

223 Full-length cDNA clones provide information about intron and exon st

224 agenesis was performed to generate three new cDNA clones (pSHEV-1, pSHEV-2, and pSHEV-3) which differ

225 tr1 by transfection with a recombinant hCtr1 cDNA clone reduced endogenous hCtr1 mRNA levels, whereas

226 Using an NPHV consensus cDNA clone, replication was not observed in primary equi

227 ional analyses of the CHV-1/EP713 infectious cDNA clone, reported here, define the requirements for p

228 Partial cDNA clones represented two groups, NMTase A and NMTase

229 Incyte GeneAlbum 1-6, which contains 65,873 cDNA clones representing 33,515 individual genes.

230 Microarray analysis, including cDNA clones representing most UniGene clusters from 12p1

231 Thirty randomly selected cDNA clones representing the differentially regulated ge

232 tudy of their subcellular location using the cDNA clone resources of TAIR.

233 Sequence analysis of genomic DNA and cDNA clones revealed nine paralogous genes tightly clust

234 cDNA cloning revealed six SAS1B splice variants, each co

235 ies and highlights discrepancies between any cDNA clone sequence and its expected reference sequence.

236 Direct cDNA cloning showed that mouse DESC1 encodes a multidoma

237 From cDNA clones showing rhizome-enriched expression, express

238 Two distinct cDNA clones showing sequence homology to higher-plant pe

239 identify the RNA activators, we developed a cDNA cloning strategy based on stringent affinity of RNA

240 A 1,086-bp reactive cDNA clone that encoded a protein of 362 amino acids wit

241 MSPE was used to isolate a novel full-length cDNA clone that encodes a 66-kDa murine G+C-rich promote

242 was screened resulting in the isolation of a cDNA clone that encodes a 738 amino acid protein (SmSmad

243 in-conjugating enzyme, have isolated a human cDNA clone that may function as a signal peptidase and h

244 Based on this screening, we identified a cDNA clone that was allowing yeast cells to grow in the

245 A cDNA clone that was isolated encoded a protein that was

246 A libraries for the isolation of full-length cDNA clones that are not yet available in the public dom

247 s transformed with a human cDNA library, and cDNA clones that rescued growth at low K(+) concentratio

248 KCC1 gene, we mapped the 5' end of the KCC1 cDNA, cloned the corresponding genomic DNA, and identifi

249 he mutagenesis of the CHV-1/EP713 infectious cDNA clone to define the requirements for p29 and p48 cl

250 unya fever (CHIKF) pandemic, we used an EILV cDNA clone to design a chimeric virus containing the chi

251 toprotein (AFP) genes by hydridizing labeled cDNA clones to HpaII and MspI digests of DNA from differ

252 In this paper, we utilized genomic and cDNA cloning to elucidate the sequence of the 5'-region

253 generated from all these myotrophin-specific cDNA clones translate in vitro to a 12-kD protein.

254 R products representing assemblies of BAC or cDNA clones typically require maintenance, propagation,

255 d dimers of the Broccoli aptamer into a SINV cDNA clone using sites in nsP3 (genomic RNA), the 3'UTR

256 om cultured cells and identified full-length cDNA clones using amino acid sequences from internal pep

257 a strategy for high-throughput sequencing of cDNA clones using the transposon Tn5.

258 Screening with a microarray containing 864 cDNA clones using wild-type and brn-3b (-/-) retinal cDN

259 cDNA cloning, using a mouse retina library or RT-PCR fro

260 on developed to help automate the process of cDNA clone validation.

261 The other three cDNA clones varied in length, but contained identical op

262 teristics expected of the mu 3 receptor, the cDNA clone was expressed in a heterologous system.

263 A PtaAGP6 full-length cDNA clone was expressed in bacteria.

264 The infectious cDNA clone was further used to generate a luciferase ZIK

265 RNA transcribed from the full-length cDNA clone was highly infectious upon transfection into

266 The FL14676485 cDNA clone was isolated from a 43(HIV) lambda ZAP Escher

267 Using a candidate gene approach, a cDNA clone was isolated that was predicted to encode the

268 A full-length LapN cDNA clone was isolated, and the deduced sequence had 77

269 specific cDNA library and a full-length NEC3 cDNA clone was isolated.

270 of these cleavage sites in an infectious FCV cDNA clone was lethal for the virus, indicating that the

271 The cDNA clone was obtained and used to generate an RNAi con

272 cDNA microarray containing 7,712 macrophage cDNA clones was used to compare the transcriptional prof

273 our previously described infectious ZIKV-RGN cDNA clone, we identified a natural polymorphism in the

274 mparative genomic DNA sequence analysis, and cDNA cloning, we found that the mouse clade B cluster at

275 Three distinct 2DL4 cDNA clones were analyzed: one encoding the "conventiona

276 tion of XyG, and partial sequences of 10,000 cDNA clones were determined.

277 ssion subtraction cDNA library from which 11 cDNA clones were found by differential dot blot hybridiz

278 Nine hundred and sixty-nine cDNA clones were found to be differentially expressed be

279 Infectious cDNA clones were generated from an epizootic subtype IC

280 Full-length cDNA clones were identified encoding two candidate enzym

281 nal genes in the 881-kb contig; 11 groups of cDNA clones were identified in addition to those for S2-

282 Transcripts of cDNA clones were infectious when they were mechanically

283 at had been characterized previously, 14 new cDNA clones were isolated as part of this work.

284 Three cDNA clones were isolated from a porcine parotid cDNA li

285 on library and three distinct immunoreactive cDNA clones were isolated.

286 s for reverse transcriptase-PCR, and several cDNA clones were isolated.

287 the nucleotide differences between these two cDNA clones were not critical for replication.

288 sis of human draft genomic DNA, and multiple cDNA clones were obtained.

289 Over 20,000 cDNA clones were partially sequenced from a normalized (

290 apsid and ORF3 proteins, indicating that the cDNA clones were replication competent.

291 On the basis of these Cmu2 markers, 22 cDNA clones were selected from an epigonal organ cDNA li

292 To characterize this library, 25,277 cDNA clones were sequenced and aligned with various data

293 RNA transcripts of mutant poliovirus cDNA clones were transfected into HeLa cells.

294 ption-polymerase chain reaction (RT-PCR) and cDNA cloning were conducted by conventional procedures.

295 of the specific mRNA sequences (assayed with cDNA clones) were in the (A)+ class.

296 We previously isolated a partial soybean cDNA clone whose transcript abundance is increased upon

297 oarrays were generated that carry about 8500 cDNA clones with approximately 6000 obtained from mammar

298 s, we constructed a series of BUNV S segment cDNA clones with deletions in the 3' and/or 5' UTR and t

299 rsion of our strategy that can be applied to cDNA clones with large cloning vectors, thereby overcomi

300 Transfection of CVB3 cDNA clones with the 5'-terminal deletions into HeLa cel