ライフサイエンスコーパス: cRNA

1 cRNA generated from PC-enriched (PC(+)) and PC-depleted

2 cRNA was synthesized from cellular RNA and hybridized to

3 cRNAs encoding full-length Mst1, and N- and C-terminal c

4 on of these mutant cRNAs and wild type Kv1.1 cRNA into Xenopus oocytes exerted a potent dominant nega

5 -1alpha/cav-1beta) zebrafish and human cav-1 cRNAs, revealing nonredundant and evolutionarily conserv

6 amma(2) (gamma(2)-N' with 837 bp) and rho(1) cRNAs were coinjected in Xenopus oocytes.

7 R1(100)+NR2A cRNAs as compared with NR1(100) cRNA alone, there is little or no effect of the NR2A sub

8 Co-injection of CFTR and Kir1.1a cRNA into Xenopus oocytes lead to the expression of K+ s

9 s ruled out by comparing MBDs with alpha(1C)-cRNA alone (4.7 +/- 0.1 ms) with beta(3xo) (14.3 +/- 1.1

10 we coinjected wild-type or mutant Kv beta 2 cRNAs and Kv1.4 cRNA in Xenopus laevis oocytes.

11 same manner as KCNQ1+KCNEx (where x=1 or 2) cRNA coinjection.

12 beta, gamma subunits) with syntaxin 1A or 3 cRNAs in Xenopus oocytes.

13 tive site; no information is available on 3' cRNA binding.

14 A template reveals a binding site for the 3' cRNA at the dimer interface.

15 ved upon hybridization of radiolabeled (35S) cRNA probes to thin sections of reproductive tissues (ma

16 ild-type or mutant Kv beta 2 cRNAs and Kv1.4 cRNA in Xenopus laevis oocytes.

17 t was induced by injection of Cx50 or Cx45.6 cRNA.

18 potentials, was observed in (Cx56 + Cx45.6) cRNA-injected oocytes compared with Cx56 cRNA-injected o

19 paired Xenopus oocytes injected with Cx31.9 cRNA, demonstrated junctional currents indicative of gap

20 A cRNA probe identifying both mRNAs showed that the transc

21 ly specific affinity-purified antibody and a cRNA probe to generate a detailed mapping of BDNF immuno

22 milar "dose" dependence: they plateaued at a cRNA ratio (MiRP1:Kv4.2) of 13:1, with half-maximum effe

23 non-concatenated receptors expressed from a cRNA ratio of 1:1:5 coding for alpha4, beta2, and delta

24 s and embryo development by microinjecting a cRNA that encodes a constitutively active (Ca(2+)-indepe

25 After injecting oocytes with NBCe1-A cRNA (Day 0), we measured NBC current (I(NBC)) by two-el

26 ction of a mutated or deleted residue 1 of a cRNA chloramphenicol acetyltransferase reporter construc

27 .3 angstrom, in the presence or absence of a cRNA or vRNA template.

28 staining, while coinjection of transferrin-a cRNA partially restored these defects.

29 ith in situ hybridization techniques using a cRNA probe to the exon encoding mature rat BDNF protein.

30 0.2% current reduction at -140 mV for WT:AAA cRNA ratios of 4:1, 3:1, 2:1, 1:1, and 1:2, respectively

31 are unknown; further, little is known about cRNA promoter conformations.

32 After cRNA injection of K(+)-transporter genes into Xenopus oo

33 However, a similar dataset, in which all cRNA identities and relative levels are known prospectiv

34 s; and (iii) coinjection of mammalian alpha1 cRNA with cRNA encoding either of the two Xenopus beta s

35 at arise after injection of mammalian alpha1 cRNAs (alpha(1C) and alpha(1E)); (ii) coinjection of a X

36 Amplified cRNA probes mixed with a universal standard were hybridi

37 Amplified cRNA was analyzed using 22,000-gene microarrays (Agilent

38 entified by microarray analysis of amplified cRNA from SPEM, and surface mucous cells were isolated b

39 subcloned into the vector, pcDNA3.1(-), and cRNA transcribed from the BCIRK1 cDNA clone was injected

40 Using antibodies and cRNA probes specific for alpha1A channels, we found that

41 icroorganisms.We also demonstrated cDNA- and cRNA-labeling and fragmentation with this method.

42 based on expression of neuronal markers and cRNA microarray analyses.

43 oocytes with guard cell protoplast mRNA and cRNA for KAT1, an inward K(+) channel expressed in guard

44 cDNA (three new data sets) and cRNA hybridization (four existing data sets) data were c

45 , the RNAP-bound 3' termini of both vRNA and cRNA exist in two conformations, corresponding to the pr

46 nitored single, surface-immobilized vRNA and cRNA initiation complexes in real-time.

47 found in virions or the NA-specific vRNA and cRNA levels in infected cells.

48 new surface binding site for the 3' vRNA and cRNA promoters on FluPol, referred to as the mode B site

49 racterize a binding site for the 3' vRNA and cRNA promoters.

50 binding domain located on both the vRNA and cRNA strands, is this RNA bound when double or single st

51 se functions, FluPol interacts with vRNA and cRNA using conserved promoter elements at the 5' and 3'

52 well as 3' deletion mutants of both vRNA and cRNA, nonviral RNA, and hybrid viral/nonviral RNA, were

53 ion was confirmed using additional antisense cRNA or oligo-cDNA probes complementary to different reg

54 35S-Labeled sense and antisense cRNA that recognizes rat CD44 standard form was used as

55 y with a digoxigenin (DIG)-labeled antisense cRNA probe.

56 Antisense cRNAs for ex5 hybridized with mRNAs in cell bodies, wher

57 After digoxigenin-labeled antisense cRNAs had been transcribed from hybridization-positive c

58 IRP binding is abrogated when APP cRNA probe is mutated in the core IRE domain (Delta4 bas

59 In Xenopus oocytes expressing AQP2 cRNAs, single-channel water permeabilities of mutants L2

60 cytes microinjected with either AQP7 or AQP9 cRNA exhibited increased transport of (73)As(III).

61 y of Xenopus oocytes injected with aquaporin cRNA by measuring the rate of swelling in hypotonic solu

62 Oocytes injected with wild-type AQY1 cRNAs exhibit high Pf values, whereas oocytes injected w

63 f values, whereas oocytes injected with AQY1 cRNAs from laboratory strains exhibit low Pf values and

64 ments (vRNAs) through intermediates known as cRNAs.

65 We used antisense (AS) cRNA to test the contribution of xKv3.1 to the maturatio

66 r each DNA microarray project and associated cRNA target information are stored in a MySQL relational

67 of oocytes injected with NtCBP4 and AtCNGC1 cRNAs induced inward rectified, noninactivating K(+) cur

68 Xenopus laevis oocytes injected with AtCNGC2 cRNA demonstrate cyclic-nucleotide-dependent, inward-rec

69 ane patches of oocytes injected with AtCNGC2 cRNA.

70 xpressed, and approximately 3600 background, cRNAs.

71 ntisense oligonucleotide and excess rat beta cRNA rescued expression of alpha1 Ca2+ channel currents;

72 ly is restored by coinjection of human beta4 cRNA or, surprisingly, by mutant cRNA encoding beta4 sub

73 ified, reverse transcribed, and biotinylated cRNA hybridized to the human high-density oligonucleotid

74 s isolated and used to generate biotinylated cRNA for hybridization to a custom 1,600-rat gene DNA mi

75 To this end, we generated biotinylated cRNA pools from livers of Plg(o) mice and controls befor

76 To this end, we generated biotinylated cRNA probes from livers of age-matched infants with the

77 We generated pools of biotinylated cRNA from livers of 14 infants with biliary atresia and

78 t aquaporin-1 is a specific water channel by cRNA expression studies in Xenopus oocytes and by functi

79 al characteristics of receptors expressed by cRNA or native retina mRNAs.

80 ed to 5 microM 5-aza-dC for 96 h followed by cRNA hybridization to an oligonucleotide microarray (Aff

81 n transporters (SERTs) in Xenopus oocytes by cRNA injection and measure 5-hydroxytryptamine (5-HT) tr

82 wt) and mutant alpharENaCs were performed by cRNA expression in Xenopus oocytes and by reconstitution

83 In Xenopus oocytes, microinjected Calx cRNA induces calcium uptake like that of its homolog, th

84 activity and activated with either CA-CaMKII cRNA or by SrCl(2), similar rates and incidence of devel

85 neurons that had been microinjected with CB1 cRNA.

86 was also frequently observed when K+ channel cRNA was injected at the equator.

87 ymerase structure with a bound complementary cRNA 5' end that exhibits a major rearrangement of the s

88 ual amounts of mutant and wild-type connexin cRNA, mimicking the heterozygous condition.

89 beling; the other was added to the copyRNAs (cRNAs) before hybridization.

90 Gene expression in each diabetic corneal cRNA was assessed against pooled cRNA from 7 to 9 normal

91 enomic RNA (vRNA), but not the corresponding cRNA or mRNA, were specifically reduced by the inhibitor

92 nt capacitance change with pure forms of CRP/cRNA while responses reduced considerably in presence of

93 At a critical CRP:cRNA ratio of 2:1, the capacitance response was dramatic

94 muM) in pure forms, but low affinity for CRP:cRNA ratio of 2:1 (K(d)=8.58 muM).

95 A) strands in pure form and co-mixtures (CRP:cRNA=0:1, 1:0, 1:1, 1:2 and 2:1).

96 nses reduced considerably in presence of CRP:cRNA in co-mixtures (1:1 and 1:2) because of the binding

97 ne uptake is significantly increased in Cx38 cRNA-injected oocytes in the absence of external calcium

98 Injection of Cx38 cRNA or Cx38 antisense oligonucleotides (to increase or

99 ocytes injected with similar amounts of CX50 cRNA.

100 Injection of Cx56 cRNA induced a slowly activating, nonselective cation cu

101 .6) cRNA-injected oocytes compared with Cx56 cRNA-injected oocytes.

102 s, including addition of phospholipase Czeta cRNA, which mimics natural fertilization.

103 In contrast, oocytes injected with D47A cRNA did not form gap junctional channels when paired ho

104 ally active mRNAs and of replicating diverse cRNA or vRNA templates at levels compatible with viral i

105 in Xenopus oocytes injected with human EAAT1 cRNA.

106 ting ENaC activity, we co-expressed rat ENaC cRNA (alpha, beta, gamma subunits) with syntaxin 1A or 3

107 13:1, with half-maximum effects at estimated cRNA ratios of 2 to 4.

108 untreated rats, ciliary neurotrophic factor cRNA labeling density was high in the olfactory nerve, p

109 immobilized aptamers had strong affinity for cRNA (K(d)=1.98 muM) and CRP molecules (K(d)=2.4 muM) in

110 Transcript abundance from cRNA hybridizations to Affymetrix microarrays can be use

111 To investigate its transport function, cRNA encoding GmN70 was expressed in Xenopus laevis oocy

112 transcripts such as PAN RNA and beta-globin cRNA exhibit two-component exponential decay kinetics in

113 current as much as 10-fold depending on HERG cRNA concentration.

114 tudy, we utilized both in vivo (heterologous cRNA expression in Xenopus laevis oocytes) and in vitro

115 th calcium-ionomycin, ionomycin, or hPLCzeta cRNA microinjection.

116 nized by the additional coinjection of Hsc70 cRNA in a concentration-dependent fashion.

117 whereas a higher amount of coinjected Hsp70 cRNA (30 ng) decreased mENaC functional and surface expr

118 t, coinjection of a moderate amount of Hsp70 cRNA (10 ng) increased the functional and surface expres

119 xpression with coinjection of 10 ng of Hsp70 cRNA was antagonized by the additional coinjection of Hs

120 amide response in oocytes expressing HtFaNaC cRNA.

121 Radiolabeled human cRNA probes were used to map the distribution of the two

122 xamined using transfected fibroblasts and in cRNA-injected voltage-clamped Xenopus oocytes, show that

123 ion was analyzed by a biotinylation assay in cRNA-injected Xenopus laevis oocytes.

124 urrents and choline transport are evident in cRNA-injected oocytes and significantly enhanced by the

125 EAAC1 modulation was studied in cRNA-injected Xenopus oocytes by measuring [3H]L-glutama

126 case, including the ability (i). to initiate cRNA synthesis de novo on both plus- and minus-stranded

127 bi-ionic conditions depended on the injected cRNA concentration.

128 on of Ih in the pyloric network, we injected cRNA of PAIH, a lobster gene that encodes Ih, into rhyth

129 d the complementary replicative intermediate cRNA using several specific binding sites; however, the

130 uses different initiation strategies on its cRNA and vRNA promoters.

131 single-strand genomic RNA (viral RNA) or its cRNA.

132 a chromosome 17 library with a specific K13 cRNA probe.

133 tes co-injected with mesophyll mRNA and KAT1 cRNA produced I(Kin) that was not inhibited by ABA.

134 om cardiac myocytes was probed with a 2.5-kb cRNA transcribed with T7 RNA polymerase from the clone N

135 Deletions at the 3' L cRNA and 5' L vRNA termini were also observed, and the p

136 letions at the 5' termini of the S, M, and L cRNAs suggests that the 3'-deleted vRNAs may not be repl

137 Labeled cRNA derived from TG-isolated total RNA was hybridized t

138 ng in situ hybridization with an 35S-labeled cRNA probe.

139 situ hybridization was used with 35S-labeled cRNA probes for the different ionotropic receptor subuni

140 yes were cut and hybridized with 35S-labeled cRNA probes specific for the glucocorticoid receptor, mi

141 The purified biotin-labeled cRNA samples were hybridized to microarray chips (GeneCh

142 vitro transcribed to produce biotin-labeled cRNA.

143 a high-sensitivity digoxigenin (DIG)-labeled cRNA in situ hybridization protocol to determine the exp

144 o high-sensitivity digoxigenin (DIG)-labeled cRNA in situ hybridization to determine the expression o

145 A for Northern blots and digoxigenin-labeled cRNA for in situ hybridization.

146 situ hybridization using digoxigenin-labeled cRNA probes and an alkaline phosphatase-conjugated anti-

147 he present study we used digoxigenin-labeled cRNA probes for the vesicular glutamate transporters, VG

148 with a catalytic subunit digoxigenin-labeled cRNA was performed on embryonic day 20 and newborn kidne

149 ed with radiolabeled and digoxygenin-labeled cRNA probes for alpha-synuclein, parkin, and UCH-L1 mRNA

150 situ hybridization with isotopically labeled cRNA probes showed that trkB and trkC mRNAs were express

151 reverse transcription, generation of labeled cRNA (target) through in vitro transcription, and hybrid

152 specific and non-specific binding of labeled cRNA to surface-bound oligonucleotides on microarrays.

153 The resultant labeled cRNA from TG isolated total RNA was hybridized to gene m

154 echnique using digoxigenin and (35)S-labeled cRNA probes to analyze, in detail, the expression of ER

155 u hybridization using specific (35)S-labeled cRNA probes.

156 replicate hybridizations of a single labeled cRNA target from three distinct experimental paradigms,

157 Labeled cRNAs were hybridized with U95Av2 GeneChips (Affymetrix)

158 Expression of LdNT2 cRNA in Xenopus oocytes significantly augmented their ab

159 ybridization was performed using full-length cRNA probes labeled with 35S-UTP.

160 idization was performed by using full-length cRNA probes labeled with 35S-UTP.

161 Expression of LmPOT1 cRNA in Xenopus laevis oocytes revealed LmPOT1 to be a h

162 nal expression of ENaC, alphabetagamma mENaC cRNAs were coinjected into Xenopus oocytes with Apx sens

163 Expression of mNBC3 cRNA in Xenopus laevis oocytes demonstrated that the pro

164 , to the terminal residues of both the model cRNA and vRNA promoters.

165 ted pppApG synthesis internally on the model cRNA promoter, whereas it initiated pppApG synthesis ter

166 t current requires approximately 5-fold more cRNA to elicit a half-maximal response.

167 e sequences were hybridized to labeled mouse cRNA producing highly concordant data (average R(2) = 0.

168 n injection of increasing amounts of M(2)-MR cRNA.

169 human beta4 cRNA or, surprisingly, by mutant cRNA encoding beta4 subunits incapable of binding to Ca(

170 nd water transports on the amounts of mutant cRNA injected was identical exponential buildups (k = 19

171 ter injection of the gamma(2) subunit mutant cRNA containing a N-terminal fragment, GABA-induced rho(

172 Co-injection of the mutant cRNAs with the wild-type cRNA did not affect the functio

173 Co-injection of these mutant cRNAs and wild type Kv1.1 cRNA into Xenopus oocytes exer

174 this hypothesis in oocytes injected with NCC cRNA with or without WNK4 cRNA.

175 idization histochemistry (ISHH) with a novel cRNA probe.

176 rvical spinal cords by using ISHH with novel cRNA probes specific for the mRNA encoding functional GH

177 ed two-fold by co-injection of NR1(100)+NR2A cRNAs as compared with NR1(100) cRNA alone, there is lit

178 The amount of cRNA and viral RNA increased to that observed for untrea

179 both increase sigmoidally with the amount of cRNA injected, but current requires approximately 5-fold

180 s induced by oocytes with similar amounts of cRNA for Cx56.

181 this study can accelerate the association of cRNA molecules, can stimulate strand displacement, and c

182 added a fixed poly(A) tail to the 3' end of cRNA.

183 Expression of cRNA encoding K(V)1.6 in Xenopus oocytes also generated

184 Hybridization of cRNA probes for trkB or trkC showed a time-dependent red

185 lymerase and establish whether initiation of cRNA and viral RNA (vRNA) differed.

186 of CLC-0 can be achieved by co-injection of cRNA encoding the transmembrane domain along with Escher

187 n showed a slight reduction in the levels of cRNA, viral RNA, and mRNA populations on the first day p

188 channels due to localized microinjection of cRNA, a naturally polarized (animal/vegetal side) distri

189 In oocytes microinjected with 5 ng of cRNA, average Pf values (in cm/s x 10(-3)) were 0.67 +/-

190 r to be expressed mostly around the point of cRNA injection when injected either into the animal or v

191 Moreover, the apparent potency of cRNA for transport depends on 5-HT concentration.

192 tem that catalyzes the unprimed synthesis of cRNA and vRNA using 50-nucleotide-long RNA templates.

193 s not required for the unprimed synthesis of cRNA and vRNA.

194 Broad zones of cRNA hybridization in the mitral cell layer became incre

195 Lastly, co-expression of cRNAs encoding residues 1-361 and 362-647 of mouse PLCze

196 The synthesis of cRNAs and vRNAs is initiated without a primer, in contra

197 Using a Xenopus oocyte cRNA expression system, we have evaluated the molecular

198 ration, were hybridized with blue cone opsin cRNA for quantitative analysis of the blue cone pattern.

199 Xenopus oocytes injected with Limulus opsin cRNA did not evoke light-sensitive currents after incuba

200 d Limulus rhodopsin expressed from a cDNA or cRNA from these systems.

201 atic conversion of mRNA into labeled cDNA or cRNA.

202 infection due to increased levels of mRNA or cRNA species.

203 ected by expression of dominant negative p85 cRNA.

204 nopus laevis oocytes microinjected with PCFT cRNA, uptake of 2, like that of Pmx, was electrogenic.

205 Xenopus oocytes injected with pCLC5 cRNA exhibited outwardly rectifying whole cell currents

206 opus laevis oocytes by microinjection of PDS cRNA or in Sf9 cells following infection with PDS-recomb

207 f Xenopus laevis oocytes injected with PIC30 cRNA demonstrated PIC30 mediated transport of picloram a

208 xpressing PLCbeta1 by microinjecting a Plcb1 cRNA significantly perturbed the duration and frequency

209 njected with either wild-type or mutant pNCT cRNA, indicating that the enhanced taurine transport act

210 tic corneal cRNA was assessed against pooled cRNA from 7 to 9 normal corneas.

211 ains were sectioned and hybridized with a PR cRNA probe.

212 ppropriate (matching) nucleotides to produce cRNA products from heteropolymeric and other homooligome

213 In situ hybridization using an R-Ras3 cRNA probe revealed high levels of R-Ras3 transcripts in

214 ction of oocytes injected with TrkA receptor cRNA, but not in uninjected or mock-injected oocytes.

215 responding residue in NET, and the resulting cRNA were expressed in Xenopus oocytes.

216 genomic RNA (vRNA) and the antigenomic RNA (cRNA), but not viral mRNA.

217 3' terminus, producing a complementary RNA (cRNA) intermediate, which serves as a template for the s

218 A polymerase (RNAP) via a complementary RNA (cRNA) intermediate.

219 Radioactive complementary RNA (cRNA) probes were prepared from cDNAs specific for alpha

220 -which proceeds through a complementary RNA (cRNA) replicative intermediate, and requires oligomeriza

221 tive binding with CRP and complementary RNA (cRNA) strands in pure form and co-mixtures (CRP:cRNA=0:1

222 NA polymerase to generate complementary RNA (cRNA), which then was used to hybridize Affymetrix GeneC

223 positive-strand influenza virus genome RNA (cRNA) and influenza virus gRNA were drastically suppress

224 ibed into positive sense complementary RNAs (cRNAs) and viral messenger RNAs (mRNAs) inside infected

225 of in vitro transcribed complementary RNAs (cRNAs) into Xenopus oocytes.

226 of vRNAs and virus complementary-sense RNAs (cRNAs) began to decline.

227 anner dependent on the amount of R482T/S187T cRNA added, consistent with the idea that the active for

228 eplicate GeneChips (hybridized with the same cRNA), we found that 95.6% of data points lie within the

229 ttachment, that could be restored by securin cRNA rescue.

230 rring, we cloned and sequenced the S-segment cRNA/mRNA from ribavirin-treated or untreated cells from

231 sing digoxigenin-labeled antisense and sense cRNA probes to human APOE.

232 study, in situ hybridization with anti-sense cRNA riboprobe was used to show expression of POMC mRNA

233 plicates it by synthesizing a positive-sense cRNA intermediate, which is copied back into vRNA.

234 histochemistry (ISHH) with a novel sensitive cRNA probe.

235 ly reconstituted by co-injection of separate cRNA constructs encoding the N-terminal transmembrane an

236 of transport and current to increasing SERT cRNA injection and mutant co-expression.

237 rase (RdRP) activities that synthesize short cRNAs by using cellular or viral RNAs as templates.

238 mily 26 (anion exchanger)-member 9 (SLC26A9) cRNA, promoted WNK4 autophosphorylation and increased NC

239 When we co-injected UT-A1 with snapin cRNA in Xenopus oocytes, urea influx was significantly i

240 ization histochemistry using monkey-specific cRNA probes.

241 in situ hybridization, using monkey-specific cRNA probes.

242 clease protection assay and species-specific cRNA probes, we measured mRNA expression levels of andro

243 full-length, but not alternatively spliced, cRNA into Xenopus oocytes resulted in the expression of

244 t a full-length copy of vRNA is made, termed cRNA, and then this cRNA is copied to produce vRNA.

245 Expression of TgAT cRNA in Xenopus laevis oocytes increased adenosine uptak

246 The cRNA derived from the CA1, CA3, and dentate gyrus region

247 The cRNA encodes a 1043-amino acid membrane protein that, wh

248 ues absent in the vRNA promoter) allowed the cRNA template strand to reach further into the active si

249 the secondary structures of the vRNA and the cRNA panhandles on the basis of solution nuclear magneti

250 initiation and initiation conformations, the cRNA promoter exhibited very limited dynamics.

251 For the cRNA panhandle, a contiguous stem helix with a protonate

252 d influenza virus RNA transcription from the cRNA promoter but not from the vRNA promoter in a report

253 e influenza virus RNA transcription from the cRNA promoter.

254 tasis (diseased controls) and hybridised the cRNA against oligonucleotide-based gene chips.

255 Expression of the cRNA in Xenopus laevis oocytes revealed strong transport

256 wo residues in the proximal 3' region of the cRNA promoter (residues absent in the vRNA promoter) all

257 ain is essential for the accumulation of the cRNA replicative intermediate in infected cells.

258 tructure of monomeric FluPol(A) bound to the cRNA template reveals a binding site for the 3' cRNA at

259 Using the cRNA-based reverse-genetics system developed for IBDV, w

260 generated recombinant IPNV strains using the cRNA-based reverse-genetics system.

261 Xenopus oocytes injected with the cRNA for both Ostalpha and Ostbeta, but not each separat

262 lammonium (TEA) in oocytes injected with the cRNA-encoding rOCT1A was increased 16-fold over that in

263 The cRNAs for eight isoforms were generated by in vitro tran

264 ries of mutant cDNAs was constructed and the cRNAs for all three subunits were expressed in Xenopus o

265 Expression of this cRNA in Xenopus oocytes revealed that the strongest tran

266 Expression of this cRNA, which does not increase intracellular Ca(2+), indu

267 of vRNA is made, termed cRNA, and then this cRNA is copied to produce vRNA.

268 tions hybridized with sense p55 and p75 TNFR cRNA probes was comparable to background.

269 Conversion to cRNA involves a target amplification step that overcomes

270 umbers of its respective reverse-transcribed cRNA.

271 We injected in vitro transcribed cRNA encoding AQPxlo into Xenopus oocytes for functional

272 ytes were injected with in vitro transcribed cRNA encoding wild-type mouse Cx50 (Cx50wt), wild-type r

273 tivity of detection with reverse-transcribed cRNAs is as low as 100 copies.

274 bridization with digoxigenin-labeled pro-TRH cRNA probe.

275 Incubating TrkA cRNA-injected oocytes with the transcriptional inhibitor

276 oplasmic face) of oocytes injected with TrkA cRNA, but not in uninjected or mock injected oocytes.

277 ction of the mutant cRNAs with the wild-type cRNA did not affect the function of the wild-type AQP2.

278 ined using a digoxigenin-labeled DNPI/VGLUT2 cRNA probe in the present study to determine which, if a

279 When co-expressed (via cRNA injections) with alpha(1B) and beta(3) subunits in

280 for expression in Xenopus laevis oocytes via cRNA injection.

281 nimal impact on mRNA and complementary vRNA (cRNA) but results in a dramatic loss of vRNA in a segmen

282 ybridized with mRNAs in cell bodies, whereas cRNAs for ex21 with mRNAs in both cell bodies and dendri

283 n, alpha1-EGFP, alone or in combination with cRNA of GABAA receptor beta2, gamma2, or beta2+gamma2 su

284 i) coinjection of mammalian alpha1 cRNA with cRNA encoding either of the two Xenopus beta subunits fa

285 with nonisotopic in situ hybridization with cRNA probes for glutamic acid decarboxylase 65 (GAD65) a

286 analyzed by using in situ hybridization with cRNA probes.

287 rine 11K Affymetrix GeneChip hybridized with cRNA from the p53 temperature-sensitive cell line, Vm10.

288 ltage clamp of Xenopus oocytes injected with cRNA encoding kainate receptor subunits, we have observe

289 Xenopus oocytes injected with cRNA encoding mKir4.2 displayed a large inwardly rectify

290 ase C (PKC) in Xenopus oocytes injected with cRNA encoding the cardiac (exon 5-) CFTR Cl- channel iso

291 ake studies in Xenopus oocytes injected with cRNA encoding this protein demonstrated transport proper

292 Oocytes co-injected with cRNA for mKir4.2 and Kir5.1, a protein that does not for

293 Xenopus oocytes were injected with cRNA of this fusion protein, alpha1-EGFP, alone or in co

294 s, Xenopus laevis oocytes were injected with cRNA of wild-type or mutant (R482T) BCRP.

295 Injection of oocytes with cRNA containing mutations of serine 187 in the ATP-bindi

296 rm duplexes of higher thermal stability with cRNA than cDNA, although destabilized compared to duplex

297 ed comparisons between chips hybridized with cRNAs prepared from an identical starting RNA population

298 Oocytes were injected with cRNAs for alphabetagamma-ENaC with or without cRNA for I

299 ive current in Xenopus oocytes injected with cRNAs of rat alpha-, beta-, and gamma-ENaC.

300 RNAs for alphabetagamma-ENaC with or without cRNA for IKKbeta.

301 injected with NCC cRNA with or without WNK4 cRNA.

302 injected with a 1:1 mixture of mutant and WT cRNA.

303 sured in Xenopus oocytes injected with zSMCT cRNAs by measurement of intracellular Na(+) concentratio