ライフサイエンスコーパス: calibrant

1 al of natural Si isotopic composition as the calibrant.

2 sing the reference standard (RS) material as calibrant.

3 is a strong candidate as a negative ion mode calibrant.

4 was due to loss of HIV DNA from the 8E5 cell calibrant.

5 application, because of its dependence on a calibrant.

6 ans of ratio counting using a colloidal gold calibrant.

7 nds, that is, the compound of interest and a calibrant.

8 has been limited by the lack of appropriate calibrants.

9 lies on mass differences instead of internal calibrants.

10 milar chemical properties between lipids and calibrants.

11 ix, indicating no need to use matrix-matched calibrants.

12 ion manipulations (SLIM) using three sets of calibrants.

13 sed as suitable for value assigning such DNA calibrants.

14 108% for all cereals matrices using external calibrants.

15 of labeled metabolites using a mixture of RT calibrants.

16 sociated with pRSETMON-02 as opposed to gDNA calibrants.

17 tter alternative to conventional seed powder calibrants.

18 eptides to the isotopically labeled internal calibrants.

19 the detected species that serve as effective calibrants.

20 ge biomolecules that can then be used as CCS calibrants.

21 minal residues and provide valuable internal calibrants.

22 accuracy MS and MS/MS spectra using separate calibrant and analyte regions on the target plate, avoid

23 ismatching while the same molecular class of calibrant and analyte was maintained yielded larger erro

24 ass standard without the necessity of mixing calibrant and analyte.

25 fication of the 50 compounds using a generic calibrant and mass calibration achieved an average error

26 pass calibration methods, which require both calibrants and analytes to experience the same number of

27 Stable isotope-labeled surrogate calibrants and internal standards were used to achieve m

28 N(2)) and Omega(He) values for the drug-like calibrants and two diastereomers were measured using a d

29 as well as the laborious nature of requiring calibrants and unknown compounds to be subjected to iden

30 -MS instrumentation relies on the use of CCS calibrants, and here, we present data from a family of p

31 ccurate calibration with a minimal number of calibrants, and the ability to extract unknown parameter

32 re analyzed at the same TW conditions as the calibrants, and we observed anomer splitting at pathleng

33 n CCS values, which can impart biases if the calibrants are not structurally similar to the analytes.

34 These calibrants are often internal and behave in a fashion si

35 Current calibrants are sometimes not designed to satisfy these r

36 Such calibrants are suitable to determine the reduced mobilit

37 neration of bespoke fluorescent protein (FP) calibrants, assays to determine protein concentration an

38 ration levels were analyzed together with 41 calibrants at 6 known concentrations by the laboratories

39 In this paper, we introduce a new IM calibrant (CCS and K0) using singly charged sodium adduc

40 ification of target concentration in primary calibrant certified reference materials (CRMs), both lam

41 for suitable ion mobility spectrometry (IMS) calibrant compounds is ongoing and necessitates the use

42 age of the methods presented here is that no calibrant compounds need to be added to the mobile phase

43 ure component relative to "internal affinity calibrants", compounds of known affinity for the target.

44 area and plotting the area ratio against the calibrant concentrations.

45 Since all calibrants consist of the same species and differ only i

46 obtain sufficiently high number of internal calibrants covering the entire ranges of mass-to-charge

47 sing an internal standard curve and need for calibrant diluent, and takes into account the individual

48 100-500 nmol/mol, with NO and NO2 reactants/calibrants diluted down from standards with nominal mole

49 urthermore, high method recoveries indicated calibrants do not need to undergo clean-up to account fo

50 pDNA calibrants exhibited better performance characteristic i

51 ce liquid chromatography, is also a suitable calibrant for CCS estimations.

52 his work establishes a new type of inorganic calibrant for IM-MS allowing sizing, structural analysis

53 e suitability of IsoF as a negative ion mode calibrant for IMS applications.

54 ifs, ultimately enabling the use of only one calibrant for these compounds.

55 However, currently available calibrants for CCS measurement using TWIM are predominan

56 Li, Na, K, Rb, Cs; R = aryl, Et; n = 1-6) as calibrants for electrospray ionization (ESI) TWIMS.

57 es multiple ion isolations of an analyte and calibrants for internal calibration at each stage in a M

58 tudy of large biomolecules, however reliable calibrants for large anions have not been available.

59 cy and reproducibility of PCs and PEs as CCS calibrants for phospholipids against different classes o

60 organic compounds that are commonly used as calibrants for qNMR spectroscopy: benzoic acid, dimethyl

61 Atmospheric moisture can contaminate calibrants for quantitative nuclear magnetic resonance (

62 However, a suitable calibrant has yet to be accurately analyzed and agreed u

63 stigations for a negative ion detection mode calibrant have been more limited.

64 Many positive ion mode calibrants have been investigated, whereas investigation

65 injection and trapping of the internal mass calibrant (i.e., poly(ethylene glycol) with a 1000 Da av

66 eveloped calibration curves using common CCS calibrants [i.e., tetra-alkylammonium salts, polyalanine

67 qHNMR using a single, nonidentical internal calibrant (IC).

68 rge effects is to introduce an internal mass calibrant (IMC) using a dual electrospray ionization (ES

69 ose of the healthy hemisphere as an internal calibrant in addition to routinely used external standar

70 show that dextran, already widely used as a calibrant in high performance liquid chromatography, is

71 nriched metalloprotein standard for use as a calibrant in species-specific isotope dilution analysis

72 l(2)O(3)) has been widely used as a pressure calibrant in static high-pressure experiments and as a w

73 ope-labeled acyl-CoAs were used as surrogate calibrants in the same matrix.

74 Such calibrants include buffer solutions, "anchor" molecules

75 r phospholipids against different classes of calibrants, including polyalanine (PolyAla), tetraalkyla

76 ins some question regarding how selection of calibrants influences TWIMS CCS measurements.

77 n "unknown" concentration without the use of calibrants, internal standards, or a calibration curve.

78 r analyte quantitation that does not rely on calibrants, internal standards, or calibration curves bu

79 ffects that would result from the spiking of calibrants into the sample.

80 This report presents a study of calibrant ion influence on CCS determination via TWIMS.

81 through the use of an internal energy-input calibrant ion that ensured reproducible energy depositio

82 Furthermore, we investigate the number of calibrant ions as well as their required m/z range in or

83 In addition, calibrant ions desorbed from a separate sample spot can

84 a method for the controlled introduction of calibrant ions in highly accurate mass measurements usin

85 well-defined series of positive and negative calibrant ions using either electrospray ionization (ESI

86 Since a single concentration of internal calibrant is necessary to calculate the study sample con

87 when conducting experiments in which a mass calibrant is needed or when multiplexing in a tandem MS

88 ignals using sucrose and ammonium sulfate as calibrants is presented.

89 The experimental results showed that the one-calibrant kinetic calibration technique can be used for

90 In open bed form, a one-calibrant kinetic calibration technique was accomplished

91 We demonstrate that structurally mismatched calibrants lead to larger errors in calibrated CCS value

92 The calibrant-loaded approach can provide both total and fre

93 se and analyte uptake between the sample and calibrant-loaded extraction phase, CL-EP, with a finite-

94 ICP-OES analysis for production of accurate calibrants may increase quantitative accuracy and give g

95 sue sections by MALDI t-wave IM-MS using CCS calibrants measured by MALDI drift cell IM-MS.

96 Mismatching of both calibrant molecular class and charge state resulted in t

97 tandardization of seminal plasma derived PSA calibrant molecular form mass concentrations and purific

98 centroided m/z values of the known, internal calibrant molecular ions versus that calculated from the

99 ltiomic mixtures, several different types of calibrants must be used to obtain the most accurate CCS

100 e of the mass error relative to the internal calibrant never exceeded 5.06 ppm.

101 This is of particular concern when calibrants of the same molecular class (e.g., carbohydra

102 accomplished by loading benzophenone3-d5 as calibrant on the C18 coating to quantify all nonpolar co

103 lysis is achieved using ICA by incorporating calibrants on a separate spot from the sample of interes

104 his study, we have chosen five potential IMS calibrants on the basis of their rating against seven cr

105 tly hindered by the requirement for external calibrants on which a working pH scale is set.

106 1-min aFT-IMS-MS sweeps without the need for calibrants or correction factors.

107 rom 0.04-2.5microg/mL and stable derivatized calibrants over 7days.

108 ed CCS values while the structurally matched calibrants, PCs and PEs, gave highly accurate and reprod

109 ose are quite unreliable, leading to missing calibrant peaks in spectra and correspondingly lower mas

110 With the set of calibrants presented here, the m/z range of 40-400 kDa i

111 t to be unsuitable for the purpose of single-calibrant quantification.

112 alculated based on the fact that 100% of the calibrant reaches the plasma through the MDG, whereas fo

113 We present the kinetics of calibrant release and analyte uptake between the sample

114 endations are made herein for each potential calibrant's suitability as a standard for the wider IMS

115 4 which demonstrate their use as a TW-IM-MS calibrant set to facilitate characterization of very lar

116 The calibration errors for the three calibrant sets (Agilent tuning mixture, polyalanine, and

117 Calibrant solutions are delivered from reservoirs fabric

118 ol to scavenge residual hydrogen peroxide in calibrant solutions provided analytically sensitive and

119 ons was established and was used to identify calibrant solutions that can be employed for the simulta

120 th this system, we have been able to deliver calibrant solutions, washing buffers, or "test" solution

121 irectly determined CCS values for 19 protein calibrant species under three solution conditions (yield

122 of mass of standard reference materials and calibrants, such as ZrH(2), 2,5-diiodothiophene, and low

123 The use of internal calibrants (the so-called lock mass approach) provides m

124 a universally applicable and robust internal calibrant, the tripeptide Asn3.

125 Using the lipid calibrants, the majority of the CCS values of several cl

126 andard, and therefore, the RS can serve as a calibrant to correct for the difference between instrume

127 o be used with a single external nitrogenous calibrant to quantify nitrogen-containing compounds of k

128 plored the validity of using "normal" charge calibrants to calibrate for charge-reduced proteins and

129 TW IM-MS) instrumentation rely on the use of calibrants to convert the arrival times of ions to colli

130 nable absolute quantification using internal calibrants to determine copies per cell of MHC antigens,

131 These instruments often rely on drift-time calibrants to perform qualitative identification.

132 isotope labeled (SIL) standards as internal calibrants to simultaneously quantify authentic analytes

133 The PSA calibrants used to create calibration curves in these as

134 ddressed and the first RI bank based on CDEA calibrants was developed.

135 Parallelism between analytes and surrogate calibrants was systematically verified in plasma across

136 antification of nucleic acid not requiring a calibrant, we demonstrated that inclusion of a carrier n

137 nation of small molecule and polypeptide CCS calibrants, we successfully determined the nitrogen CCS

138 e been corrected through the use of internal calibrants (well-characterized standards introduced with

139 Standard curves of the spiked calibrants were generated with concentrations ranging fr

140 Calibrants were made of nitrocellulose membranes doped w

141 Matrix-matched calibrants were needed to compensate for over recovery o

142 Two sets of 18 calibrants were selected for each of ESI+ and ESI-based

143 The development of phospholipid CCS calibrants will enable high-accuracy structural studies

144 -tagged peptides can further act as internal calibrants with high confidence in a PMM to identify the

145 -to-charge ratios and abundances of internal calibrants without a need for theoretical description of

146 standard oxidation-reduction potential (ORP) calibrants, ZoBell's and Light's solution.