ライフサイエンスコーパス: capacitation

1 is protein is tyrosine phosphorylated during capacitation.

2 ify phosphorylation changes occurring during capacitation.

3 the protein phosphorylation cascade of sperm capacitation.

4 oes tyrosine phosphorylation during in vitro capacitation.

5 e found to be tyrosine phosphorylated during capacitation.

6 mally required by wild-type sperm to achieve capacitation.

7 se sperm and is coupled to events regulating capacitation.

8 They also did not undergo proper capacitation.

9 rylation events that take place during sperm capacitation.

10 osine phosphorylation events associated with capacitation.

11 annels in epididymal sperm examined prior to capacitation.

12 protein tyrosine phosphorylation as well as capacitation.

13 ia to support signal transduction leading to capacitation.

14 of the sperm oviductal reservoir, and sperm capacitation.

15 ated rabbit spermatozoa and is unaffected by capacitation.

16 not occur in a medium that does not support capacitation.

17 epididymis, it cannot fertilize eggs before capacitation.

18 m membrane potential (Em) takes place during capacitation.

19 e ubiquitin-proteasome system (UPS) in sperm capacitation.

20 ns and relocalization of PKA subunits during capacitation.

21 y the targets of UPS regulation during sperm capacitation.

22 ouse sperm membrane hyperpolarization during capacitation.

23 to decrease sperm ATP such as starvation and capacitation.

24 entry pathway activated by BSA during sperm capacitation.

25 PSCs is reversed by resetting and subsequent capacitation.

26 matin accessibility features reflect lineage capacitation.

27 city in the female tract in a process called capacitation.

28 incubated in conditions that do not support capacitation.

29 c and related transcriptional changes during capacitation.

30 kalinization need for SLO3 activation during capacitation.

31 activity appeared to be dispensable for the capacitation.

32 range of distinct protein substrates during capacitation.

33 stream signalling events essential for sperm capacitation.

34 ding reverse cholesterol transport and sperm capacitation.

35 m to acquire acrosomal responsiveness during capacitation.

36 in tyrosine phosphorylation that accompanies capacitation.

37 with sperm maturation, collectively known as capacitation.

38 ed in the female tract in a process known as capacitation.

39 mal tyrosine phosphorylation associated with capacitation.

40 These are themselves shed from sperm during capacitation.

41 terol efflux, an important step during sperm capacitation.

42 otility and hyperactivation typical of sperm capacitation.

43 otein tyrosine phosphorylation of late-stage capacitation.

44 ime-dependent effects on multiple aspects of capacitation.

45 otein that is tyrosine-phosphorylated during capacitation.

46 (P<0.0001) before and, more severely, after capacitation.

47 ll as serine/threonine phosphorylated during capacitation.

48 ter leaflet of null spermatozoa before sperm capacitation.

49 co-factor-independence of GalT I-null sperm capacitation.

50 tion can occur, mammalian sperm must undergo capacitation, a process that requires a cyclic AMP-depen

51 s suitability as a biomarker for identifying capacitation, an enduring problem in the study of sperm

52 hat [Mg(2+)](i) is regulated in sperm during capacitation and acrosomal reaction, and suggest that th

53 reduced ability to fertilize eggs, although capacitation and acrosome exocytosis appear to be normal

54 Ca(2+) influx into sperm is associated with capacitation and development of hyperactivated motility.

55 mosome 6 (pi6) produce sperm with defects in capacitation and egg fertilization.

56 of eIF2alpha phosphorylation delays ribosome capacitation and favors reinitiation at ATF4 over the in

57 Sperm capacitation and fertilization are highly regulated by C

58 Events in sperm capacitation and fertilization require similar cellular

59 naling pathways regulating such processes as capacitation and flagellar motility.

60 This maturational process is called capacitation and in mouse sperm it involves a plasma mem

61 o evaluate the roles of the channel in sperm capacitation and in the inflammatory immune response of

62 s are hotspots of epigenetic dynamics during capacitation and indicate possible distinct roles of spe

63 atSper cation channel is essential for sperm capacitation and male fertility.

64 ntral role in the control of mammalian sperm capacitation and motility.

65 the Ca(2+) store, which may be regulated by capacitation and NO from the cumulus.

66 ighlights the importance of the UPS in sperm capacitation and offers opportunities to identify novel

67 ts support the proposed role of UPS in sperm capacitation and open several new lines of inquiry into

68 ouse sperm with Ca(2+) ionophore accelerated capacitation and rescued fertilizing capacity in sperm w

69 egated signaling programs that support sperm capacitation and shed light on a hitherto unforeseen cau

70 ated to play roles in spermatogenesis, sperm capacitation and sperm-egg binding in mammals.

71 Ca(2+) regulate sperm motility, chemotaxis, capacitation and the acrosome reaction, and play a vital

72 the ability to fertilize in a process called capacitation and undergo hyperactivation, a change in th

73 n cascade(s) regulating events pertaining to capacitation and/or motility in mammalian sperm and that

74 and voltage, Slo3 could be involved in sperm capacitation and/or the acrosome reaction, essential ste

75 bjected to extensive remodeling during sperm capacitation, and (ii) the UPS has a narrow range of dis

76 tes, histamine secretion by basophils, sperm capacitation, and airway pH.

77 such as immune cell respiratory burst, sperm capacitation, and cancer cell migration.

78 didymal sperm count, spermatozoa morphology, capacitation, and motility and reduced ejaculated sperm

79 ortance, the molecular mechanisms underlying capacitation are poorly understood.

80 ism attenuates alkaline shifts in pHi during capacitation as well as the ability of sperm to produce

81 HDL also supports sperm capacitation, as assessed by fertilization in vitro.

82 )/Cl(-) cotransporters (NKCC), inhibited all capacitation-associated events, suggesting that these tr

83 nt of Na(+) by choline(+) also inhibited the capacitation-associated increase in protein tyrosine pho

84 monstrated that HCO-(3) is necessary for the capacitation-associated increase in protein tyrosine pho

85 olesterol binding protein, also supports the capacitation-associated increase in protein tyrosine pho

86 protein kinase A (PK-A) is upstream of this capacitation-associated increase in protein tyrosine pho

87 r to stallion and human, PF431396 blocks the capacitation-associated increase in tyrosine phosphoryla

88 c kinase family inhibitor SU6656 blocked the capacitation-associated increase in tyrosine phosphoryla

89 Recently, it has been proposed that the capacitation-associated increase in tyrosine phosphoryla

90 activating mutation in Fer failed to undergo capacitation-associated increases in tyrosine phosphoryl

91 However, the block of capacitation-associated parameters was overcome when spe

92 n the other hand, in conditions that support capacitation-associated processes blocking hyperpolariza

93 In this experimental setting, other capacitation-associated processes such as activation of

94 of Cl(-) did not affect sperm viability, but capacitation-associated processes such as the increase i

95 Both the capacitation-associated tyrosine phosphorylation and the

96 t undergo a physiological maturation, termed capacitation, before they are able to fertilize eggs.

97 factors from wild-type sperm phenocopies the capacitation behavior of GalT I-null sperm.

98 Following capacitation, binding to suLe(A) decreased significantly

99 ltering sperm functions, including motility, capacitation, binding to the zona pellucida, binding to

100 neckless tails were motile and could induce capacitation but had no significant forward motility.

101 in wild-type sperm was maximal after 2 h of capacitation, but capacitation of sperm from spasmodic m

102 roduce capacitation in vitro or induction of capacitation by cell-permeant cAMP analogs decreased the

103 s sperm motility, an important early step in capacitation, by increasing flagellar beat frequency thr

104 icipates in signaling pathways that regulate capacitation, Cl(-) was replaced by either methanesulfon

105 The molecular basis of mammalian sperm capacitation, defined as those biochemical and functiona

106 Signaling events leading to mammalian sperm capacitation depend on the modulation of proteins by pho

107 SAAI) were processed by the proteasome in a capacitation dependent manner.

108 HCO(3)(-) current and the inhibition of the capacitation-dependent increase in protein tyrosine phos

109 (UPS), implicated in the regulation of sperm capacitation, diminished sperm release in response to al

110 Sperm capacitation, essential for fertilization, is regulated

111 Pmca4-/- sperm that had not undergone capacitation exhibited normal motility but could not ach

112 These two behavioral consequences of capacitation, exocytotic competence and altered motility

113 Naive-to-primed transition (capacitation) follows transcriptional dynamics of human

114 Capacitation for multi-lineage differentiation occurs wi

115 ulating protein tyrosine phosphorylation and capacitation, further supports the importance of PK-A in

116 the primary pathway for Ca(2+) influx during capacitation; however, emerging evidence suggests additi

117 In spermatozoa, capacitation, hyperactivation of motility and the acroso

118 n kinase A (PKA) plays a fundamental role in capacitation in all mammalian species.

119 As expected, wild-type sperm must undergo capacitation in order to bind the zona pellucida and und

120 ully fertilize an egg, it must first undergo capacitation in the female reproductive tract and later

121 lT I may function as a negative regulator of capacitation in the sperm head by suppressing intracellu

122 Sperm capacitation in vitro is highly correlated with an incre

123 Incubations known to produce capacitation in vitro or induction of capacitation by ce

124 nduced acrosome reactions without undergoing capacitation in vitro.

125 ertilize an oocyte, sperm must first undergo capacitation in which the sperm plasma membrane becomes

126 Considering that capacitation-induced hyperpolarization is mediated by SL

127 d that inhibition of its activity blocks the capacitation-induced hyperpolarization of the sperm plas

128 Slo3 is the main current responsible for the capacitation-induced hyperpolarization, which is require

129 How sperm regulate the capacitation-induced increase in carbon flux is unknown.

130 an O-linked glycosylated protein, undergoing capacitation-induced processing dependent on Ca(2+) and

131 Furthermore, the capacitation-induced reorganization of the outer acrosom

132 glycolytic activity seems to be achieved via capacitation-induced stimulation of flux through aldolas

133 sine phosphorylation that is associated with capacitation, induction of the acrosome reaction, forwar

134 Sperm capacitation involves a series of biochemical and physio

135 Sperm capacitation is a complex process that takes place in th

136 We previously demonstrated that mouse sperm capacitation is accompanied by a time-dependent increase

137 Capacitation is also accompanied by hyperpolarization of

138 Capacitation is an essential post-testicular maturation

139 erm capacitation." We have demonstrated that capacitation is associated with an increase in the tyros

140 In addition, mouse sperm capacitation is associated with the hyperpolarization of

141 This inhibitor revealed that capacitation is defined by separable events: induction o

142 tin cytoskeleton reorganization during sperm capacitation is essential for the occurrence of acrosoma

143 Sperm capacitation is essential to gain fertilizing capacity.

144 Capacitation is needed for the activation of motility (e

145 tly, this work presents strong evidence that capacitation is regulated by two parallel pathways.

146 Sperm capacitation is required for fertilization.

147 One important correlate of capacitation is the development of hyperactivated motili

148 data suggest that one aspect of mouse sperm capacitation is the selective activation of one major pH

149 probably contribute to sperm maturation and capacitation, is established during development.

150 -fold in abundance (P < 0.05) after in vitro capacitation (IVC) and 13 proteins were found significan

151 also increased the percentage of sperm with capacitation-like changes in membrane structure.

152 In a process called capacitation, mammalian sperm gain the ability to fertil

153 t increases in cAMP content occurring during capacitation may inhibit ENaCs to produce a required hyp

154 open several new lines of inquiry into sperm capacitation mechanism.

155 ounters albumin, a critical component of the capacitation medium used in in vitro fertilization proce

156 Cl(-) transport, sperm incubated in complete capacitation medium were exposed to a battery of anion t

157 cubated under conditions that do not support capacitation (NC) can become ready for an agonist stimul

158 We demonstrate in this report that capacitation of cauda epididymal mouse sperm in vitro wa

159 stigated the activity of UPS during in vitro capacitation of fresh boar spermatozoa in relation to ch

160 Interestingly, capacitation of GalT I-null sperm is independent of the

161 Transcriptome trajectory during in vitro capacitation of human naive cells tracks the progression

162 ning the requirement for PKA activity during capacitation of sperm from mice that express CalphaM120A

163 m was maximal after 2 h of capacitation, but capacitation of sperm from spasmodic mice for up to 3 h

164 els of eIF2alpha phosphorylation favor early capacitation of such reinitiating ribosomes directing th

165 hyperactivated motility, which occur late in capacitation of wild-type spermatozoa, do not develop in

166 did not show membrane hyperpolarization upon capacitation or induction with cAMP analogs.

167 shed by their morphology, ability to undergo capacitation or the acrosome reaction, and/or mitochondr

168 In this paper, we describe the capacitation phenotype of sperm lacking the long isoform

169 When sperm were subjected to 'in vitro' capacitation, photo-stimulation also increased the perce

170 Before capacitation, PKA is maintained in an inactive state whe

171 This reservoir delays capacitation, prevents polyspermy, selects a fertile pop

172 The latter beat pattern is part of the capacitation process whereby sperm prepare for the prosp

173 acellular alkalinization, are central to the capacitation process.

174 sperm were incubated in a medium supporting capacitation, proteins became tyrosine-phosphorylated in

175 tom-up proteomic approach to (i) monitor the capacitation-related changes in the sperm protein levels

176 Signaling events leading to mammalian sperm capacitation rely on activation/deactivation of proteins

177 te rapid surges in signaling cascades during capacitation remains unknown.

178 It is well established that capacitation requires Na(+), HCO(3)(-), Ca(2+), and a ch

179 At the molecular level, capacitation requires protein kinase A activation, chang

180 vation of this signaling pathway, as well as capacitation, requires bovine serum albumin (BSA) in the

181 Furthermore, these sperm show accelerated capacitation resulting in an overall in vitro fertilizin

182 revents engagement of multiple components of capacitation resulting in male infertility.

183 Sperm capacitation results in pHi increases (from 6.54 +/- 0.0

184 Sperm capacitation selectively exposed a partial von Willebran

185 During sperm capacitation SLO3 hyperpolarizes the sperm, whereas CATS

186 After capacitation, sperm beat asymmetrically with lower ampli

187 During capacitation, sperm lose a fraction of their Sias.

188 ed with biological processes including sperm capacitation, sperm motility, metabolism, binding to zon

189 rgism between S1 and S2 for abnormalities in capacitation, sperm-oolemma binding, and zona-free oocyt

190 However, after capacitation, staining in the neck decreased, and most o

191 ty is preserved in mouse sperm regardless of capacitation state but is lost in the sperm either lacki

192 its increased phosphorylation status during capacitation suggested multiple important functions for

193 cal localization did not change during sperm capacitation, suggesting that glycolysis in sperm is reg

194 the oviduct epithelium but the components of capacitation that are important for sperm release are un

195 ozoa must undergo a maturation step known as capacitation that takes place after ejaculation.

196 daptations within the female (the process of capacitation) that are initiated by agents ranging from

197 During capacitation the mouse sperm plasma membrane potential (

198 However, one of the component processes of capacitation, the ability to undergo a zona pellucida-ev

199 uterine fluid yields proton efflux, allowing capacitation, the acrosomal reaction, and oocyte fertili

200 to play a pivotal role in sperm motility and capacitation, the distinctive biochemical properties of

201 signaling events leading to mammalian sperm capacitation, the immediate activation of protein kinase

202 Upon capacitation, the PKA regulatory subunits, but not the c

203 actions as well as the early events of sperm capacitation, the remodeling of the sperm plasma membran

204 A critical regulator of capacitation, the soluble adenylyl cyclase (sAC; ADCY10)

205 In contrast, a second component process of capacitation, the transition to hyperactivated flagellar

206 During this process of capacitation, they acquire progressive motility, develop

207 Balpha system can regulate cytokine receptor capacitation through effects on the induction of downstr

208 BSA is hypothesized to modulate capacitation through its ability to remove cholesterol f

209 BSA is hypothesized to modulate capacitation through the removal of cholesterol from the

210 required for at least the initial 30 min of capacitation to produce subsequent protein tyrosine phos

211 the motility and maturation process known as capacitation to reach and fertilize the oocyte.

212 of AE in which membrane fusions occur during capacitation/transit through the cumulus, prior to any p

213 To identify proteins phosphorylated during capacitation, two-dimensional gel analysis coupled to an

214 , the increase in PK-A activity accompanying capacitation was associated with enzyme activity found i

215 at glucose is not essential for murine sperm capacitation, we demonstrated that glucose (but not lact

216 ertilize constitute the phenomenon of "sperm capacitation." We have demonstrated that capacitation is

217 After epididymal maturation, sperm capacitation, which encompasses a complex series of mole