ライフサイエンスコーパス: capsid

1 0 of HIV and the fivefold axis of the EV-A71 capsid).

2 tis B capsid protein dimers to the 120-dimer capsid.

3 of nucleic acid encapsulated into a protein capsid.

4 CP subunits) exposed on the exterior of the capsid.

5 functional antigenic determinants on the HPV capsid.

6 cally package their genomes into a preformed capsid.

7 resses generated due to the curvature of the capsid.

8 tagons that would anchor the NEC coat to the capsid.

9 oncanonical interfaces, thus stabilizing the capsid.

10 s protruding fibers covalently linked to the capsid.

11 , through which DNA is translocated into the capsid.

12 ed in an asymmetric T = 3 pseudo-icosahedral capsid.

13 corporation of the viral genome in the HSV-1 capsid.

14 h a maturation protein incorporated into its capsid.

15 ereas a pleomorphic envelope wraps the outer capsid.

16 tory, and utilizes the widely available AAV9 capsid.

17 the assembly of the hepatitis B virus (HBV) capsid.

18 assive translocation of dNTPs into the HIV-1 capsid.

19 rived from a different strain than the outer capsid.

20 has the least area and free energy, in both capsids.

21 mer-of-dimers and the appearance of 90-dimer capsids.

22 re is an appreciable population of defective capsids.

23 tigenic determinants on the surface of these capsids.

24 cated, concatemeric viral DNA into preformed capsids.

25 id (ssDNA) encapsulated within 25-nm protein capsids.

26 ading to productive egress of UL25-decorated capsids.

27 contributes to the anterograde transport of capsids.

28 rnessing the 3D structural properties of AAV capsids.

29 t allows the passage of dNTPs into assembled capsids.

30 proteins and tegument proteins that encrust capsids.

31 e find that brain accumulation of the PHP.eB capsid 1) exceeds that reported in any previous PET stud

32 Three serotype capsids, AAV1, AAV2, and AAV9, have been approved for co

33 Notably, capsids acquired envelopes at axonal varicosities and te

34 tein-protein interactions between portal and capsid, across a symmetry-mismatched interface.

35 re to proteases cleaves the peptides off the capsid, activating or "switching ON" the provector.

36 the assembly of 3 types of icosahedral viral capsids: Adeno Associated Virus serotype 2 (AAV2) and Mi

37 roscopic structures of the icosahedral MCPyV capsid and analysis of its glycan interactions via nucle

38 tron microscopy structures of purified VZV A-capsid and C-capsid, as well as of the DNA-containing ca

39 Phage G has the largest capsid and genome of any known propagated phage.

40 hibitor-induced tetramerization of the viral capsid and provided essential structural and functional

41 A organization in the multipartite BMV viral capsid and the monopartite bacteriophages MS2 and Qbeta

42 for engaging the terminase complex with the capsid and the subsequent cleavage, packaging, and stabl

43 g capsid engineering) and the ability of the capsid and transgene to avoid the host immune response (

44 terfaces are consistent with those in intact capsids and substantially different from the organizatio

45 ses to increase the volume and complexity of capsids and would provide a new approach to construct hi

46 ippage, whilst allowing its passage into the capsid, and how these processes are controlled.

47 apidly evolving 'v1' loop to bind retroviral capsids, and single mutations in this loop can dramatica

48 thesis that critical properties, such as AAV capsid antibody evasion and tropism, can be coevolved by

49 ntial impact of this genome variation on the capsid antigenicity of lineage and sublineage variants o

50 ent structure reveals atomic-level detail in capsid architecture and provides important guidance for

51 Viral capsids are dynamic assemblies that undergo controlled c

52 internal volume of the X174, G4, and alpha3 capsids are nearly identical, their genome lengths vary

53 ugh crystallographic studies show that viral capsids are static structures, accumulating evidence sug

54 py structures of purified VZV A-capsid and C-capsid, as well as of the DNA-containing capsid inside t

55 ause of the fluctuating environment in which capsids assemble and the requirement of some capsids for

56 Yet, capsids assemble rapidly with minimal trapped intermedia

57 These results offer insights into the EBV capsid assembly and a mechanism for recruiting cell-regu

58 many double-stranded DNA viruses, governing capsid assembly and genome packaging.

59 JNJ-56136379 (JNJ-6379), a capsid assembly modulator that blocks hepatitis B virus

60 The HBV capsid assembly reaction can result in a heterogeneous m

61 residues that are critical for transduction, capsid assembly, or DNA packaging.

62 he cruciality of an interface for successful capsid assembly.

63 data on site-directed mutagenesis' effect on capsid assembly.

64 ompletion of the viral replication cycle and capsid assembly.

65 identify key residues and motifs involved in capsid assembly.

66 Our reconstructions confirm that the capsid associated tegument complex is present on capsids

67 capsid-tegument interfaces involving SCP and capsid-associated tegument complexes (CATC): SCPs crown

68 ically attached DNA-translocating portal and capsid-associated tegument complexes from cryogenic elec

69 Chaotic genome-capsid associations could inhibit packaging or genome re

70 gliosides relieve this block, binding to the capsid at low pH and facilitating a late step in entry i

71 f retroviral infection, and reveal divergent capsid-based EB1 mimicry across retroviral species.

72 ormed, revealing that T = 3, T = 4 and mixed capsids between these two triangulation numbers are gene

73 To characterize RNA-capsid binding sites genome-wide within mature RNA virus

74 at vPAR-CL can readily and reliably identify capsid binding sites in genomic viral RNA by detecting c

75 In addition, GS-6207 interferes with capsid binding to the cellular HIV-1 cofactors Nup153 an

76 Moreover, some of them can act on the capsid by denaturing proteins.

77 s to have created the necessary space in the capsid by evolving the shortest R domain.

78 the inner nuclear membrane (INM) around the capsid by forming a hexagonal array.

79 l dodecamer is anchored at one vertex of the capsid by interactions with the adjacent triplexes as we

80 We have compared the two capsids by cryo-EM at 3.5 angstrom resolution.

81 human immunodeficiency virus type 1 (HIV-1) capsid (CA) protein forms a conical lattice around the v

82 against the structural proteins (SP) of the capsid can be used to monitor seroconversion in both inf

83 date the molecular-scale mechanisms of viral capsid cellular compartmentalization by TRIM5alpha.

84 es in a manner distinct from currently known capsid cofactors, influenced by pentamer composition or

85 e inner protein layer, organized as a T = 19 capsid, confines the core shell, and it is composed of t

86 man herpesviruses, VZV has a similarly sized capsid, consisting of 955 major capsid protein (MCP), 90

87 The mature retrovirus capsid consists of a variably curved lattice of capsid p

88 HIV-1 capsid core disassembly (uncoating) must occur before in

89 also shows how translocation of DNA into the capsid could be modulated by a changing mode of protein-

90 trimer interfaces as the key contributor to capsid curvature.

91 s that potently block infection by wild-type capsid, demonstrating that HIV-1 can use distinct nuclea

92 m permeabilized virions supported efficient, capsid-dependent endogenous reverse transcription to pro

93 hat this serotype-specific immune profile is capsid-dependent.

94 niques can be applied to inform and optimize capsid design.

95 terned structures that wrap around the viral capsid, despite an anomalously low affinity for the caps

96 Infection in the presence of the capsid destabilising small molecule PF-74 also induced a

97 bserve that HIV-1 remains susceptible to the capsid-destabilizing compound PF74 following nuclear imp

98 describe the first structure of herpesvirus capsids determined by sub-tomogram averaging from nuclei

99 A2 domain, the structure of an EDTA-treated capsid, determined to 2.8- angstrom resolution, suggests

100 induces a "kissing" interaction between two capsid dimers.

101 m, penetrate cellular membranes, and undergo capsid disassembly to reach an intracellular destination

102 ll-binding peptide (MBP) incorporated in the capsid displayed a reduced lung tropism and efficiently

103 During uncomplemented infections, capsids dissociated during packaging or quickly thereaft

104 ars that the ssRNA binding (R) domain of the capsid diverged evolutionarily in order to recognize the

105 a hexameric lattice-structured platform for capsid docking and recruit viral and cellular NEC-associ

106 differential scanning fluorimetry (DSF) and capsid dynamics by matrix-assisted laser desorption ioni

107 A key knowledge gap is how the capsid engages the replicated viral genome and the subse

108 e transgene cassette), vector tropism (using capsid engineering) and the ability of the capsid and tr

109 l structural proteins and is a major site of capsid envelopment.

110 h, forming similar interactions in different capsid environments while maintaining strict symmetry in

111 mpassing four predicted sites exposed on the capsid exterior.

112 iently internalized through endocytosis, but capsids fail to uncoat and accumulate within LAMP1(+) en

113 We further demonstrated that the GAd capsid fiber shared the flexibility of the HAd5 equivale

114 capsids assemble and the requirement of some capsids for disassembly before genome delivery.

115 Approaches to engineer recombinant capsids for gene therapy applications have focused on ra

116 eins of tailed bacteriophage and Herpesvirus capsids form dodecameric rings that occupy one capsid ve

117 igh-resolution structures of retrovirus-like capsids formed by Drosophila dArc1 and dArc2 that have s

118 These data demonstrate that virus-like capsid-forming properties of Arc are evolutionarily cons

119 The outer capsid forms a hexagonal lattice (triangulation number T

120 ale, we measure cruciality by changes in the capsid free-energy landscape partition function when an

121 The capsid-free viral RNA in the exosome lumen, but not the

122 ILVs with sphingosine, which prevents HSV-1 capsids from penetrating into the cytosol.

123 rpesvirus infection, egress of nascent viral capsids from the nucleus is mediated by the viral nuclea

124 fit between inner and outer shells maintains capsid function.

125 otyped by sequencing a partial region of the capsid gene.

126 lthough published structural models of viral capsids generally exhibit a high degree of regularity or

127 The VZV capsid has high thermal stability, although with relativ

128 ection.IMPORTANCE Protein shells of viruses (capsids) have evolved to undergo specific changes to ens

129 Further genetic engineering of the GAd capsid holds the promise of in vivo vector tropism modif

130 Additional targeted mutations in the HBoV1 capsid identified several residues that are critical for

131 ay even impart different structures to their capsids.IMPORTANCE The majority of viruses contain RNA g

132 capsids with non-genomic RNA containing the capsid incorporation sequence, and investigated the stru

133 ntroduction of specific changes to the outer capsid, indicating that an optimal fit between inner and

134 The potent HIV-1 capsid inhibitor GS-6207 is an investigational principal

135 edge for rational design of panserotype DENV capsid inhibitors.

136 s important guidance for the design of novel capsid inhibitors.

137 d C-capsid, as well as of the DNA-containing capsid inside the virion.

138 DMS-MaPseq reveals that the predominant RNA-capsid interaction sites favored double-stranded RNA reg

139 The capsid is an essential component of the virion and it is

140 an interface for successful assembly of the capsid is based on combinatorial entropy.

141 The outer capsid is composed of mu1-sigma3 heterohexamers which su

142 an tumor virus and demonstrate that the full capsid is required for the essential interaction with it

143 The HIV-1 capsid is the key determinant of MxB sensitivity and cof

144 ssful assembly of a closed protein shell (or capsid) is a key step in the replication of viruses and

145 nt AAV vectors has largely relied on natural capsid isolates.

146 iral ribonucleoprotein complexes outside the capsid lattice, which led to premature degradation of th

147 xamer interactions that stabilize the curved capsid lattice.

148 of the curved hexagonal NEC coat around the capsid, leading to productive egress of UL25-decorated c

149 this structural information to engineer AAV capsid libraries through saturation mutagenesis of diffe

150 or loaded with its ATP cofactor, to a 1 MDa capsid-like homo-hexacontamer, we conclude that while ph

151 t high-resolution structures of several DHBV capsid-like particles (CLPs) determined by electron cryo

152 -like particles can be converted into mature capsid-like particles by the simple addition of viral pr

153 gs suggest structural heterogeneity in virus capsids may be under-appreciated, and also points to a n

154 between protein energy simulations and viral capsid metastability.

155 Icosahedral viral capsids must undergo conformational rearrangements to co

156 We identified a capsid mutant (H41R) that loses this interaction and doe

157 Capsid mutants and imaging revealed that CLIP170 bound H

158 Additionally, we observe that certain capsid mutants are insensitive to a Nup62-mediated nucle

159 table disease, the double HVD and triple HVD/capsid mutants induce high levels of neutralizing antibo

160 itivity and cofactor binding defective HIV-1 capsid mutants P90A (defective for cyclophilin A and Nup

161 Epidemic ZIKV strains contain capsid mutations that increase Dicer binding affinity an

162 to intact HIV-1 cores or in vitro assembled capsid-nucleocapsid complexes, while EB1 did not.

163 The 2-layer capsid of reovirus provides a model system to study the

164 ing variation on the antigenicity of the HPV capsid of vaccine-relevant oncogenic HPV genotypes.IMPOR

165 at self-assembled into artificial virus-like capsids of approximately 47 nm in size.

166 tibodies that target the virus particles (or capsids) of the most common HPV cancer-causing genotypes

167 tarting from an integer multiple of 60 viral capsid or coat protein (VP) monomers.

168 y at the portal vertex of an empty precursor capsid (or procapsid) to power genome encapsidation.

169 discovery of a mutational hot spot at HBoV1 capsid position 590 that accumulated in two patients dur

170 and are incorporated during the assembly of capsid precursors called procapsids or proheads.

171 id associated tegument complex is present on capsids prior to nuclear egress.

172 The capsid protein (CA) content of the infectious viral core

173 The central cavity of the capsid protein (CA) hexamer reveals itself as a plausibl

174 sid consists of a variably curved lattice of capsid protein (CA) hexamers and pentamers.

175 despite an anomalously low affinity for the capsid protein (CA).

176 ck HBV (DHBV) as a model although DHBV has a capsid protein (CP) comprising ~260 rather than ~180 ami

177 e mosaic virus (BMV) is packaged by a single capsid protein (CP) into three types of morphologically

178 ding site bridging over three domains of one capsid protein (CP) monomer.

179 The alphavirus capsid protein (Cp) selectively packages genomic RNA (gR

180 8,280 copies of the double jelly-roll major capsid protein (MCP) p72, arranged in trimers displaying

181 ilarly sized capsid, consisting of 955 major capsid protein (MCP), 900 small capsid protein (SCP), 64

182 of 955 major capsid protein (MCP), 900 small capsid protein (SCP), 640 triplex dimer (Tri2) and 320 t

183 plays an essential role in the maturation of capsid protein (VP)-encoding mRNAs and viral DNA replica

184 molecule that disrupts the functions of HIV capsid protein and is amenable to long-acting therapy ow

185 rion structure and the identity of the major capsid protein and other predicted virion proteins, incl

186 in complex known as retromer binds to the L2 capsid protein and sorts incoming virions into the retro

187 have T = 1 symmetry with the characteristic capsid protein beta-barrels found in all the viruses in

188 F6 significantly decreased the expression of capsid protein but not that of DHX15.

189 f Balb/c mice with different combinations of Capsid protein containing VLPs either as monovalent, biv

190 mixture of intermediates extending from the capsid protein dimer to the icosahedral T = 4 capsid wit

191 diates on the assembly path from hepatitis B capsid protein dimers to the 120-dimer capsid.

192 s in the nuclear localization signal in EEEV capsid protein have an additional negative effect on vir

193 ystal structure (1.5 angstrom) of the DENV-2 capsid protein in complex with an inhibitor that potentl

194 synthesis of the N-glycan of the viral major capsid protein in PBCV-1 and establishes that a single p

195 hough with relatively fewer intra- and inter-capsid protein interactions and less stably associated t

196 Fitting of the capsid protein into densities shows the presence of a he

197 and inhibition of human Dicer enzyme by the capsid protein is a potential mechanism for this unique

198 Each capsid protein is comprised of three domains: R (RNA bin

199 ind tightly at a conserved interface between capsid protein monomers, where it interferes with capsid

200 ccessfully conjugated tHBcAg VLPs to the HIV capsid protein P24 in the cytosol.

201 multiple layers, and resolve the major outer-capsid protein p72 to higher resolution.

202 Results show the capsid protein plays a central role in the flavivirus as

203 This structure illuminates capsid protein quaternary organization, including its or

204 gh proportions of nonforming or noninfective capsid protein sequences that reduce the effective depth

205 eviously unknown role for the reovirus outer capsid protein sigma3 in limiting the induction of a nec

206 tron tomography, we show that binding of the capsid protein UL25 promotes the formation of NEC pentag

207 irus B3 (CVB3) differ by single mutations in capsid protein VP1 or VP3 and also differ in stability a

208 tified neutralizing antibodies that bind the capsid protein VP1 to block viral infection.

209 ted by the exposed apical loops of the major capsid protein VP1, a broad range of GAG oligosaccharide

210 cellular adhesion molecule 1 receptors via a capsid protein VP1-specific fivefold canyon feature, the

211 y conserved region in the N terminus of FMDV capsid protein VP2 (VP2N) was characterized using a pane

212 undles formed from the extended C-termini of capsid protein VP4B and VP4C protrude from the capsid su

213 tions in nsP3 HVD and clustered mutations in capsid protein, and tested the effects of these modifica

214 cipally target conserved epitopes within the capsid protein, escape from which results in a significa

215 The major capsid protein, gp27, is identified and found to share t

216 Mutations in the major viral capsid protein, VP1, are common in JCPyV from PML patien

217 The additional clustered mutations in capsid protein, which affect its ability to induce trans

218 ized with the antibody against the HIV-1 p24 capsid protein, while it is powered via paper-based biof

219 f NP1, and plays a role in the maturation of capsid protein-encoding mRNAs in the nucleus.

220 Unexpected findings included capsid protein-transcription factor chimeras; endonuclea

221 y/tertiary structures that interact with the capsid protein.

222 nt RNA polymerase (RdRP), capping enzyme and capsid protein.

223 he adenosine deaminase ADAR2 fused to an MS2 capsid protein.

224 d protein monomers, where it interferes with capsid-protein-mediated interactions between proteins th

225 F sequence showed homology with the putative capsid proteins (CPs) of mycoviruses.

226 previously determined, and show that all 10 capsid proteins (from four heterodimers and two homodime

227 ificant structural rearrangements induced by capsid proteins and invite the application of time-resol

228 nfectious virions requires the production of capsid proteins and other late genes, whose production i

229 (mReC), we find that the similarity of major capsid proteins and terminase large subunits further sug

230 del system to study the interactions between capsid proteins and the changes they undergo during entr

231 study reveals new insights into the roles of capsid proteins and their multiple functions during vira

232 le in the maturation of viral mRNAs encoding capsid proteins as well as in viral DNA replication.

233 Analysis of 58,023 major capsid proteins from large and giant viruses using metag

234 tially vaccinated with DNA plasmids encoding capsid proteins of different RV A types.

235 ibodies against conserved regions located on capsid proteins of RV A viruses, mice were sequentially

236 urring variation on the structure of the HPV capsid proteins of vaccine-relevant oncogenic HPV genoty

237 Human papillomavirus (HPV) L1 and L2 capsid proteins self-assemble into virions capable of ef

238 ng in a subnanometer resolution structure of capsid proteins within the virus particle.

239 We identified several additional capsid proteins, including a candidate for the prohead p

240 l neural network to identify candidate viral capsid proteins, several of which formed virus-like part

241 contain RNA genomes protected by a shell of capsid proteins.

242 and subsequently UV-crosslinked to adjacent capsid proteins.

243 By contrast, the arrangement of the capsid proteins:RNA complex, which forms the core of the

244 ular mechanism of nucleotide import into the capsid remains unknown.

245 o provided evidence that axonal transport of capsids requires the kinesin-1 molecular motor.

246 nary models may be able to elucidate complex capsid residue-residue interaction networks essential fo

247 This arises from most capsid residues not being under any strong selective pre

248 sites on the viral surface, thus increasing capsid rigidity and stability.

249 In doing so, the capsids sample a range of conformational space, with imp

250 At the capsid-scale, cruciality of an interface for successful

251 een symmetry-related VP monomers; and at the capsid-scale, we predict the cruciality of an interface

252 Such cannot be achieved by utilizing capsid sequence data alone but requires harnessing the 3

253 HBoV1 variants by cloning 29 distinct HBoV1 capsid sequences from primary human samples and by analy

254 eby preventing functional disassembly of the capsid shell in infected cells.

255 with most of the RNA genome situated at the capsid shell.

256 ensities that appear to have no contact with capsid shell.

257 The crystallographic structure of the BMV capsid shows four trypsin cleavage sites (K(65), R(103),

258 y in virus replication, suggesting these RNA-capsid sites are multifunctional.

259 encodes a protein, glycoGag, that increases capsid stability and limits APOBEC3 access to the revers

260 Capsid stability was restored by introduction of specifi

261 of the digestion time, while retaining their capsid structural integrity, B1(V) and B2(V) released a

262 invertebrate PVs with respect to host-driven capsid structure and unique as a PV showing a cation-sen

263 Capsid structure information also provides knowledge pot

264 Its capsid structure, determined by cryoelectron microscopy

265 However, the capsid structures of all three viruses are similar, with

266 led that GS-6207 tightly binds two adjoining capsid subunits and promotes distal intra- and inter-hex

267 psid protein VP4B and VP4C protrude from the capsid surface.

268 mily Siphoviridae because of its icosahedral capsid surrounded by head fibers and a non-contractile l

269 Plasticity reaches the greatest level at the capsid-tegument interfaces involving SCP and capsid-asso

270 The inhibitor-bound capsid tetramers are assembled inside virions, resulting

271 ally designed adeno-associated virus (AAV) 6 capsid that demonstrates efficiency in lung epithelial c

272 icking and conformational changes in the AAV capsid that support efficient genome transcription.

273 s passed from the tip, along the tube to the capsid that triggers passage of the DNA down the tube an

274 is little change to the shell domain of the capsid, the radiating protruding domains are flexible, a

275 gi compartment are essential for priming the capsid to support efficient AAV genome transcription.IMP

276 il tube of bacteriophage lambda connects its capsid to the tail tip.

277 and resolving structures of T = 3 and T = 4 capsids to 4 angstrom and 6 angstrom respectively.

278 hods and present new protocols for using HPV capsids to deliver non-viral DNA thereby providing an al

279 To expand their utility, we evolved AAV capsids to efficiently transduce specific cell types in

280 ential for these naturally occurring variant capsids to impact recognition by neutralizing monoclonal

281 The ability of HPV capsids to package non-viral DNA makes these a useful to

282 , we observed a defect in the ability of AAV capsids to undergo conformational changes and support ef

283 Recently, AAV2.retro, a new capsid variant capable of efficient retrograde transport

284 Curiously, albeit a large variety of HBoV1 capsid variants has been isolated from human samples, on

285 , infectivity, and immunoreactivity of HBoV1 capsid variants improve our understanding of bocaviral b

286 o PCR amplify and sequence 29 distinct HBoV1 capsid variants.

287 psids form dodecameric rings that occupy one capsid vertex and are incorporated during the assembly o

288 , binding of UL25 situated at the pentagonal capsid vertices to the NEC at the INM promotes formation

289 densovirus, despite the lack of significant capsid viral protein (VP) sequence similarity.

290 es, which sometimes looped out from ruptured capsid walls.

291 cargoes ranging from small proteins to large capsids were excluded from liquid FG-Nup droplets, but f

292 To ensure a valid comparison, the two capsids were prepared and imaged under identical conditi

293 In contrast, B3+4(V) capsids were unstable with trypsin, releasing several pe

294 nsists of a peptide insertion into the virus capsid which disrupts the virus' ability to bind to cell

295 The capsid, which we have named AAVv66, shares high sequence

296 apsid protein dimer to the icosahedral T = 4 capsid with 120 dimers.

297 near-atomic-resolution structures of the EBV capsid with an asymmetrically attached DNA-translocating

298 his study, we have assembled recombinant MS2 capsids with non-genomic RNA containing the capsid incor

299 ormation is found inside the assembled viral capsids, with RNA density conserved even at the center o

300 ck in an entropic trap and essentially every capsid would follow a unique assembly path.