ライフサイエンスコーパス: carboxysome

1 h which metabolites presumably pass into the carboxysome.

2 to be structurally characterized from a beta-carboxysome.

3 l beta-cyanobacterial genomes is part of the carboxysome.

4 egularly spaced subcellular compartment, the carboxysome.

5 isCO), the major enzyme component inside the carboxysome.

6 ernatively, EutG may recycle NADH within the carboxysome.

7 in other organisms) as shell proteins of the carboxysome.

8 ated in a specialized organelle known as the carboxysome.

9 idual building blocks within the icosahedral carboxysome.

10 odel of the internal arrangement of the beta-carboxysome.

11 nobacteria with Form-IB Rubisco contain beta-carboxysomes.

12 tep in the biogenesis of cyanobacterial beta-carboxysomes.

13 Small granular bodies were also seen within carboxysomes.

14 nsity frequently connects granules to nearby carboxysomes.

15 stered into polyhedral protein bodies called carboxysomes.

16 e compared with those of intact and ruptured carboxysomes.

17 f the enzyme into polyhedral organelles, the carboxysomes.

18 rotein shell that is related to the shell of carboxysomes.

19 The mutant does not possess carboxysomes.

20 pecialized proteinaceous compartments called carboxysomes.

21 me architectural similarities to prokaryotic carboxysomes.

22 o bicarbonate, supporting carbon fixation in carboxysomes.

23 s, via Rubisco condensation, to fully formed carboxysomes.

24 onic anhydrase into microcompartments called carboxysomes.

25 istinct from CsoS2A in the assembly of alpha-carboxysomes.

26 nside proteinaceous microcompartments called carboxysomes.

27 sequence similarity to the shell proteins of carboxysomes (a polyhedral organelle involved in autotro

28 An example is the carboxysome, a bacterial microcompartment for CO(2) fixa

29 a process occurs during the formation of the carboxysome, a bacterial microcompartment that assembles

30 The best-characterized BMC is the carboxysome, a central part of the carbon-concentrating

31 We have constructed a synthetic mimic of the carboxysome, a cyanobacterial carbon-fixing organelle.

32 ototypical bacterial microcompartment is the carboxysome, a protein shell for sequestering carbon fix

33 ploy cryo-electron tomography to image alpha-carboxysomes, a pseudo-icosahedral microcompartment resp

34 s encode homologues of shell proteins of the carboxysome, an organelle shown (in other organisms) to

35 This process requires the carboxysome, an organelle-like proteinaceous microcompar

36 We show that CcmN localizes to the carboxysome and is essential for carboxysome biogenesis.

37 th its apparent genomic association with the carboxysome and its constituents.

38 alizations of the outer protein shell of the carboxysome and its internal cargo.

39 m for BMC-associated metabolism, joining the carboxysome and metabolosome.

40 The carboxysome and other bacterial microcompartments (BMCs)

41 tulated pore-forming protein CsoS1D with the carboxysome and show how it may modulate function.

42 reover, RbcX appears as one component of the carboxysome and shows a dynamic interaction with Rubisco

43 Given that carboxysomes and architecturally related bacterial organ

44 ary and structural differences between alpha-carboxysomes and beta-carboxysomes, we found that the tw

45 ALC localizes to carboxysomes and exhibits ATPase activity.

46 mutant of McdB has altered association with carboxysomes and influences carboxysome enzyme content.

47 Fundamental to engineering carboxysomes and other BMCs for applications in plant sy

48 Within the scope of synthetic biology, carboxysomes and other BMCs hold even greater potential

49 insights into the assembly and formation of carboxysomes and paves the way for engineering carboxyso

50 ns suggest a functional relationship between carboxysomes and polyphosphate granules.

51 r components, including thylakoid membranes, carboxysomes and polyribosomes, as well as phages, insid

52 rs, which could function as adaptors to link carboxysomes and provide for stable transport by the Mcd

53 ioengineering applications, the formation of carboxysomes and their structural composition, stoichiom

54 eleton to control the spatial arrangement of carboxysomes and to optimize the metabolic process of ca

55 obacteria with Form-IA Rubisco contain alpha-carboxysomes, and cyanobacteria with Form-IB Rubisco con

56 that much of the enzyme is sequestered into carboxysomes, and that the genes for the enzyme, cbbL an

57 formation of polyhedral organelles known as carboxysomes, and two encode proteins that appear distan

58 es molecular transport through the shells of carboxysomes, applicable to other BMCs.

59 observed that the shell proteins of the beta-carboxysome are able to assemble in plant chloroplasts i

60 wed that some of the shell components of the carboxysome are produced, which may explain the presence

61 Carboxysomes are a family of bacterial microcompartments

62 Carboxysomes are a family of bacterial microcompartments

63 Carboxysomes are a paradigm of self-assembling proteinac

64 Carboxysomes are bacterial microcompartments that encaps

65 Carboxysomes are bacterial microcompartments that enhanc

66 Carboxysomes are bacterial microcompartments that functi

67 Carboxysomes are BMCs containing ribulose-1,5-bisphospha

68 Carboxysomes are bounded by a proteinaceous outer shell

69 Carboxysomes are capsid-like, CO(2)-fixing organelles th

70 Carboxysomes are compartments in bacterial cells that pr

71 Typically, alpha-carboxysomes are genetically encoded as a single operon

72 Because beta-carboxysomes are obligately expressed, heterohexamer for

73 Carboxysomes are organelle-like polyhedral bodies found

74 Carboxysomes are polyhedral bodies consisting of a prote

75 Carboxysomes are polyhedral inclusion bodies that play a

76 Carboxysomes are protein microcompartments found in cyan

77 Carboxysomes are proteinaceous bacterial microcompartmen

78 Carboxysomes are proteinaceous organelles that encapsula

79 obal carbon cycle, little is known about how carboxysomes are spatially regulated.

80 Carboxysomes are thought to locally concentrate CO(2) in

81 led biosynthesis and ordered organization of carboxysomes are vital to the CO2-fixing activity of cya

82 ker protein CsoS2 plays an essential role in carboxysome assembly and Rubisco encapsulation.

83 Here, we describe the stages of carboxysome assembly and the requisite gene products nec

84 and decreases the genetic load required for carboxysome assembly in heterologous systems.

85 n these observations, we propose a model for carboxysome assembly in which the shell and the internal

86 Carboxysome assembly involves a series of protein-protei

87 We propose that the principles of carboxysome assembly that we have uncovered extend to di

88 These results provide a new model for carboxysome assembly with implications for photosyntheti

89 ng of protein-protein interactions governing carboxysome assembly, but also establish a platform to s

90 o the role of RbcX from Syn7942 in mediating carboxysome assembly.

91 The alpha-carboxysome-associated ortholog, CsoCbbQ, from Halothiob

92 that this protein, which we refer to as the carboxysome-associated PII protein (CPII), represents a

93 A (or CsoS4A), from the two known classes of carboxysomes, at resolutions of 2.4 and 2.15 angstroms.

94 l RuBisCO to be encapsulated into modern-day carboxysomes, bacterial organelles central to the cyanob

95 indicating a close coordination between beta-carboxysome biogenesis and photosynthetic electron trans

96 ar mechanism underlying Rubisco assembly and carboxysome biogenesis will provide essential informatio

97 er enriched CO(2) and impaired regulation of carboxysome biogenesis, without affecting growth rate.

98 izes to the carboxysome and is essential for carboxysome biogenesis.

99 ential knowledge for us to modulate the beta-carboxysome biosynthesis and function in cyanobacteria.

100 However, little is known about how carboxysome biosynthesis and spatial positioning are phy

101 We demonstrated that beta-carboxysome biosynthesis is accelerated in response to i

102 Carboxysome breakdown is reversed by environmental condi

103 M composed of one or two HCO3(-) pumps and a carboxysome, but its functionality has not been examined

104 Of particular interest are the carboxysomes (CBs), which sequester the CO(2)-fixing enz

105 lular localization of the ALC, its effect on carboxysome/cell ultrastructure in Fremyella diplosiphon

106 We show here that carboxysomes, CO(2)-fixing microcompartments encoded by

107 Mutations were also recovered in ccmK4, a carboxysome coat protein homologue, and me, the decarbox

108 Carboxysomes compartmentalize the enzyme ribulose-1,5-bi

109 Our study indicates that carboxysome composition is probably more complex than wa

110 mic evidence have important implications for carboxysome composition, structure, and function.

111 Carboxysomes consist of a protein shell that encapsulate

112 A functional carboxysome consisting of an intact shell and cargo is e

113 It is present in all alpha-carboxysome containing bacteria and has homologs in alga

114 Protein domains native to the carboxysome core were used to encapsulate foreign cargo

115 based on protein domain interactions in the carboxysome core.

116 As a metabolic module for carbon fixation, carboxysomes could be transferred to eukaryotic organism

117 vision defects, glycogen limitation, lack of carboxysomes, deteriorated thylakoids and accumulation o

118 sizes and enzyme packings of alpha- and beta-carboxysomes each constitute an optimal compartmentaliza

119 Carboxysomes enclose the enzymes Rubisco and carbonic an

120 xation in C4 plants or in the cyanobacterial carboxysome, enhances the activity of inefficient enzyme

121 association with carboxysomes and influences carboxysome enzyme content.

122 Carboxysomes exhibited greater size, shape, and composit

123 Capsids, carboxysomes, exosomes, vacuoles and other nanoshells ea

124 rotein shell; prominent examples include the carboxysome for CO2 fixation and catabolic microcompartm

125 Well-characterized BMCs include carboxysomes for CO2-fixation, and propanediol- and etha

126 e especially useful for developing synthetic carboxysomes for plant engineering.

127 X gene affects Rubisco abundance, as well as carboxysome formation and spatial distribution.

128 our knowledge of the principles that mediate carboxysome formation and structural modulation.

129 Well-defined Rubisco assembly and carboxysome formation are pivotal for efficient CO(2) fi

130 Deletion of the peptide abolishes carboxysome formation, indicating that its interaction w

131 the individual roles of CsoS2B and CsoS2A on carboxysome formation.

132 lly replaces four gene products required for carboxysome formation.

133 ppear distantly related to those involved in carboxysome formation.

134 condense Rubisco and enable efficient alpha-carboxysome formation.

135 Here, studies on the alpha-carboxysome from Halothiobacillus neapolitanus demonstra

136 carbonic anhydrase encapsulation into alpha-carboxysomes from Halothiobacillus neapolitanus.

137 to explore the possibility of producing beta-carboxysomes from Synechococcus elongatus PCC7942, a mod

138 the three-dimensional structure of purified carboxysomes from Synechococcus species strain WH8102 as

139 cessful purification and characterization of carboxysomes from the marine cyanobacterium Prochlorococ

140 Some models of carboxysome function depend on specific contacts between

141 nowledge, the first direct comparison of the carboxysome function from alpha-cyanobacteria and beta-c

142 l function of these species and that similar carboxysome function may be possessed by each carboxysom

143 a novel RuBisCO chaperone integral to alpha-carboxysome function.

144 mity alone may not be the key determinant in carboxysome function.

145 hotomixotrophic growth, and the abundance of carboxysome gene (rbcL, ccmK1, ccmL) and ndhF4 transcrip

146 Similar results were observed for carboxysome gene transcription and assembly, as assayed

147 We found that carboxysomes generally cluster into distinct groups with

148 The carboxysome genes are located adjacent to cbb genes, whi

149 In addition to carboxysome genes, two novel genes (Tcr_1019 and Tcr_131

150 ccmK4 situated in a locus remote from other carboxysome genes.

151 in tobacco chloroplasts by transforming nine carboxysome genetic components derived from a proteobact

152 the different Rubisco forms present in each carboxysome had almost identical kinetic parameters.

153 Complicating the study of cyanobacterial carboxysomes has been the inability to obtain homogeneou

154 ggesting that the S. enterica organelles and carboxysomes have a related multiprotein shell.

155 i-permeability, and catalytic improvement of carboxysomes have inspired rational design and engineeri

156 teria, bacterial microcompartments, known as carboxysomes, improve the efficiency of photosynthesis b

157 l Rubisco and progress toward synthesizing a carboxysome in chloroplasts.

158 ces, we introduce a strategy that mimics the carboxysome in cyanobacteria by utilizing microcompartme

159 cells undergoing division evenly segregated carboxysomes in a nonrandom process.

160 eter, localization, and mobility patterns of carboxysomes in cells depend sensitively on the microenv

161 Expression of carboxysomes in E. coli resulted in the production of ic

162 re sufficient for heterologous expression of carboxysomes in Escherichia coli, opening the door to de

163 We have aligned and averaged carboxysomes in several size classes from the 3D tomogra

164 The widely accepted models for the role of carboxysomes in the carbon-concentrating mechanism of au

165 Likewise, the spatial organization of carboxysomes in the cell correlates with the redox state

166 nd organizational variability of single beta-carboxysomes in the model cyanobacterium Synechococcus e

167 Using electron cryotomography, we examined carboxysomes in their native states within intact cells

168 We found that while the sizes of individual carboxysomes in this organism varied from 114 nm to 137

169 Here, we generate morphologically correct carboxysomes in tobacco chloroplasts by transforming nin

170 Finally, we observed carboxysomes in various stages of assembly, as well as f

171 s a bacterial microcompartment (BMC) called 'carboxysome' in which RuBisCO is co-encapsulated with th

172 Characterization of purified synthetic carboxysomes indicated that they were well formed in str

173 ic electron flow impairs the accumulation of carboxysomes, indicating a close coordination between be

174 eding diffusion of CO(2) into and out of the carboxysome interior.

175 nts establish the feasibility of introducing carboxysomes into chloroplasts for the potential compart

176 sign and synthetic engineering of functional carboxysomes into higher plants to improve photosynthesi

177 on the viability of transplanting functional carboxysomes into the C3 chloroplast.

178 obacterial CO2 -concentrating mechanism, the carboxysome, into chloroplasts is an approach to enhance

179 The carboxysome is a bacterial microcompartment that enhance

180 The carboxysome is a bacterial microcompartment that functio

181 The carboxysome is a bacterial organelle found in all cyanob

182 The carboxysome is a complex, proteinaceous organelle that p

183 The carboxysome is a protein-based organelle for carbon fixa

184 The cyanobacterial carboxysome is a proteinaceous microcompartment that pro

185 The carboxysome is a specialized bacterial organelle that en

186 The carboxysome is believed to function as a microcompartmen

187 e also demonstrate that CO2 retention in the carboxysome is necessary, whereas selective uptake of HC

188 The number of carboxysomes is increased in 4 under high-CO2 conditions

189 How Rubisco complexes pack within the carboxysomes is unknown.

190 We propose that carboxysome-like organelles help bacteria conserve certa

191 In S. enterica, this carboxysome-like structure (hereafter referred to as the

192 o form aggregated Rubisco complexes with the carboxysome linker protein CcmM35 within tobacco (Nicoti

193 ny chemoautotrophs, products of the expanded carboxysome locus include CbbO and CbbQ, a member of the

194 indicates that, similar to DNA segregation, carboxysome maintenance systems employ Walker-box protei

195 DNA partition-like ParA-ParB system mediates carboxysome maintenance, called McdA-McdB.

196 hought to localize differentially within the carboxysome matrix.

197 ut a relatively fast catalytic rate within a carboxysome microcompartment.

198 eport the first structural insights into the carboxysome of Prochlorococcus, the numerically dominant

199 Prototypes of the BMCs are the carboxysomes of autotrophic bacteria.

200 These structures, typified by carboxysomes of cyanobacteria and many chemoautotrophs,

201 The first BMCs discovered were the carboxysomes of cyanobacteria.

202 al and cluster in the central cytoplasm, the carboxysomes of MED4 are smaller.

203 he organization of this lattice in beta-type carboxysomes of the freshwater cyanobacterium Synechococ

204 The alpha-carboxysome operon is embedded in a cluster of additiona

205 nzyme, cbbL and cbbS, are part of a putative carboxysome operon.

206 lyzing the genomic regions around alpha-type carboxysome operons, we characterize a protein that is c

207 s are found to co-occur near or within alpha-carboxysome operons.

208 or the transfer, regulation, and assembly of carboxysomes, or any of the myriad of functionally disti

209 skeletal protein ParA specifically disrupted carboxysome order, promoted random carboxysome segregati

210 some, through rubisco network formation, and carboxysome organization.

211 re on average approximately 250 RuBisCOs per carboxysome, organized into three to four concentric lay

212 determined the crystal structure of an alpha-carboxysome PCD-like protein from the chemoautotrophic b

213 ent in PCD enzymes is disrupted in the alpha-carboxysome PCD-like protein.

214 At the optimal carboxysome permeability, contributions from CO2 scaveng

215 Transmembrane bicarbonate transporters and carboxysomes play key roles in accumulating bicarbonate

216 has been the inability to obtain homogeneous carboxysome preparations.

217 nism Escherichia coli by expressing a set of carboxysome protein-encoding genes.

218 Carboxysomes, protein-coated organelles in cyanobacteria

219 Carboxysomes provide significantly elevated levels of CO

220 In this study, structure and function of carboxysomes purified from wild type Halothiobacillus ne

221 chemical analysis indicates that if they are carboxysome related, they are not functional, i.e., do n

222 More broadly, the reengineered carboxysomes represent a proof of concept for a domain f

223 nserved PCD-like protein, renamed here alpha-carboxysome RuBisCO assembly factor (or acRAF), is a nov

224 disrupted carboxysome order, promoted random carboxysome segregation during cell division, and impair

225 The carboxysome self-assembles from thousands of individual

226 The carboxysome serves as an ideal model system for testing

227 tural basis of selective permeability of the carboxysome shell and can be used to design modification

228 recent progress in the re-engineering of the carboxysome shell and core to offer a conceptual framewo

229 the potential for gated transport across the carboxysome shell and reveals a new type of building blo

230 ver, we find striking similarity between the carboxysome shell and the structurally characterized, ev

231 arbon cycle, the molecular mechanisms behind carboxysome shell assembly remain unclear.

232 expression data, were used to identify a new carboxysome shell component, CsoS1D, in the genome of Pr

233 Genes coding for carboxysome shell components and the encapsulated protei

234 We studied the regulation of beta-carboxysome shell composition by investigating the BMC-H

235 The carboxysome shell consists of three structurally charact

236 lysis suggests that CsoSCA exists within the carboxysome shell either as a homodimer or as extended f

237 mes that collectively enhance catalysis, the carboxysome shell enables the generation of a locally el

238 We find selectivity at the carboxysome shell is not necessary; there is an optimal

239 The carboxysome shell is roughly 800 to 1400 angstroms in di

240 tion of dodecamers in solution suggests that carboxysome shell permeability may be dynamically attenu

241 necessary; there is an optimal non-specific carboxysome shell permeability.

242 ated with their transition through the major carboxysome shell protein CcmK2.

243 The most abundant carboxysome shell protein contains a single bacterial mi

244 nerate a population-level description of the carboxysome shell protein, RuBisCO, and CcmM isoform loc

245 e-dimensional crystal structures of multiple carboxysome shell proteins, revealing a hexameric unit a

246 he three-dimensional structures of hexameric carboxysome shell proteins, which self-assemble into mol

247 s inorganic carbon transporters and the beta-carboxysome shell proteins.

248 ss and the specific interactions between the carboxysome shell proteins.

249 The carboxysome shell serves as a semi-permeable barrier for

250 The model requires the carboxysome shell to be a major barrier to CO2 efflux an

251 ocalization of carbonic anhydrase within the carboxysome shell with Rubisco, cyanobacteria are able t

252 ion levels, catalytic rates, permeability of carboxysome shell) for efficient carbon fixation.

253 ic building block for incorporation into the carboxysome shell, and the trimers further dimerize, for

254 racts with CsoCbbO and is a component of the carboxysome shell, the first example of ATPase activity

255 lity of approximately 10(-2) cm/s across the carboxysome shell, the shell proteins reflect enough CO(

256 rboxysomes and paves the way for engineering carboxysome shell-based nanoreactors to recruit specific

257 eptide is essential for interaction with the carboxysome shell.

258 plausible, preliminary atomic models for the carboxysome shell.

259 of a recombinantly produced simplified beta-carboxysome shell.

260 Here, we build large, intact carboxysome shells (over 90 nm in diameter) in the indus

261 Carboxysome shells contain multiple BMC-H paralogs, each

262 esent a structural characterization of alpha-carboxysome shells generated from recombinant systems, w

263 Here we synthetically engineer alpha-carboxysome shells using minimal shell components and de

264 ability properties of metabolite channels in carboxysome shells.

265 tic operon in Escherichia coli that produces carboxysome shells.

266 atalytic rates measured for CsoSCA in intact carboxysomes suggest that the protein shell acts as a ba

267 bacillus neapolitanus and Nitrobacter agilis carboxysomes suggest that the structures are either icos

268 The resulting streamlined carboxysomes support photosynthesis.

269 Photosystem II activity and carboxysome synthesis are lost in the tocopherol mutants

270 Although both strains possess carboxysomes that are polygonal and cluster in the centr

271 osahedral protein microcompartments known as carboxysomes that encapsulate multiple copies of the CO(

272 membrane-bound organelles of eukaryotes, in carboxysomes the interior of the compartment forms first

273 The carboxysome, the cyanobacterial BMC for CO(2) fixation,

274 In alpha-carboxysomes, the disordered linker protein CsoS2 plays

275 In the case of the alpha-carboxysome, there is little molecular insight into prot

276 lity at the level of a metabolic module, the carboxysome, through rubisco network formation, and carb

277 for passage of metabolites in and out of the carboxysome to enhance CO(2) fixation.

278 d averaging of the computationally extracted carboxysomes to minimize the missing data effects.

279 any bacteria employ a protein organelle, the carboxysome, to catalyze carbon dioxide fixation in the

280 arboxysome function may be possessed by each carboxysome type.

281 These include the two carboxysome types and a divergent set of metabolosomes,

282 ns indicate that the possession of different carboxysome types does not significantly influence the p

283 The two carboxysome types have arisen through convergent evoluti

284 Interestingly, both carboxysome types showed a response to cytosolic Ci, whi

285 on A (RpaA), exhibit a striking breakdown of carboxysomes under certain environmental conditions.

286 s a multimolecular structure that mimics the carboxysome used by some photosynthetic bacteria to fix

287 It was found that H. neapolitanus carboxysomes vary widely in size and mass as shown by cr

288 Surprisingly, no more than one partial carboxysome was ever observed per cell.

289 ferences between alpha-carboxysomes and beta-carboxysomes, we found that the two strains are remarkab

290 Here, fluorescently tagged carboxysomes were found to be spatially ordered in a lin

291 Because the isolated P. marinus MED4 carboxysomes were free from contaminating membrane prote

292 more definitively, purified H. neapolitanus carboxysomes were re-examined by cryo-electron tomograph

293 ll of a cellular microcompartment called the carboxysome, where it converts HCO(3)(-) to CO(2) for us

294 The central CO2-fixing machinery is the carboxysome, which is composed of an icosahedral protein

295 S2B isoform were capable of producing intact carboxysomes, while those with only CsoS2A were not.

296 e/oxygenase (RubisCO) sequestered within the carboxysome with sufficiently high concentrations of its

297 nthesis and subcellular localization of beta-carboxysomes within a model cyanobacterium, Synechococcu

298 Rubisco-containing microcompartments called carboxysomes within cells.

299 The results show that the addition of carboxysomes without other CCM components substantially

300 hereas selective uptake of HCO3 (-) into the carboxysome would not appreciably enhance energetic effi