ライフサイエンスコーパス: cardiac troponin C

1 ortant for the proper regulatory function of cardiac troponin C.

2 ' iodoacetamido-tetramethylrhodamine-labeled cardiac troponin C.

3 n is initiated when Ca2+ binds to site II of cardiac troponin C.

4 the calcium binding affinity of fluorescent cardiac troponin C.

5 ponin I(129-166) to the regulatory domain of cardiac troponin C.

6 tivity in the absence of calcium-bound human cardiac troponin C.

7 I interacts with the NH2-terminal domain of cardiac troponin C.

8 inding myofilament proteins, including cTnC (cardiac troponin C), a major cytosolic Ca(2+) buffer, we

9 ression of CARP in cardiomyocytes suppresses cardiac troponin C and atrial natriuretic factor transcr

10 , the NH2- and COOH-terminal domains in free cardiac troponin C and cardiac troponin C bound cardiac

11 the calcium-saturated complex formed between cardiac troponin C and cardiac troponin I.

12 minal deletion mutants formed a complex with cardiac troponin C and troponin I that exhibited the sam

13 type or mutated cTnI together with wild-type cardiac troponin C and troponin T.

14 , together with alpha-tropomyosin (Tpma) and cardiac troponins C and I (Tnni3), forms a calcium-sensi

15 nt distance from the calcium binding site in cardiac troponin C, and do not affect either the binding

16 te genes such as Nkx2.5, GATA4, Tbx5, dHAND, cardiac troponin C, and SM22-alpha.

17 as used to determine incorporation of mutant cardiac troponin C ( approximately 21%) into the KI-TnC-

18 beling of the endogenous Cys-84, labeling of cardiac troponin C at a novel engineered Cys-53 with 2-(

19 Cardiac troponin C belongs to the EF-hand superfamily of

20 minal domains in free cardiac troponin C and cardiac troponin C bound cardiac troponin I-(1-80)DD tum

21 (15)N transverse relaxation rates for intact cardiac troponin C bound to either cardiac troponin I(1-

22 in the regulatory domain of Ca(2+)-saturated cardiac troponin C bound to the isolated N-domain of car

23 ease the calcium affinity of the N-domain of cardiac troponin C by facilitating the movement of helic

24 um binding to the single, regulatory site of cardiac troponin C by measuring the rates of calcium-med

25 CaM (4 cTnC) is a calmodulin--cardiac troponin C chimeric protein containing the first

26 The highest-ranked up-regulated gene, cardiac troponin C, codes for a neuronal calcium-binding

27 new hypertrophic cardiomyopathy mutations in cardiac troponin C (cTnC) (A8V, C84Y, E134D, and D145E)

28 cTnI(Delta2-11) interact with the N lobe of cardiac troponin C (cTnC) and that phosphorylation at Se

29 the complex formation between IAANS-labeled cardiac troponin C (cTnC) and the two cTnI mutants.

30 tion, TnI(1-32) interacts with the N-lobe of cardiac troponin C (cTnC) and thus is positioned to modu

31 Mutations in TNNC1-encoded cardiac troponin C (cTnC) are a relatively rare cause of

32 Two novel mutations (G159D and L29Q) in cardiac troponin C (CTnC) associate their phenotypic out

33 ms by which a double mutation (E59D/D75Y) in cardiac troponin C (CTnC) associated with dilated cardio

34 c cardiomyopathy-associated mutant D145E, in cardiac troponin C (cTnC) C-domain, causes generalised i

35 The solution structure of cardiac troponin C (cTnC) challenges existing structure/

36 binary complex with the C-terminal domain of cardiac troponin C (cTnC) comprising residues 81-161.

37 and cTn containing WT cardiac troponin T/I + cardiac troponin C (cTnC) D65A (a site II inactive cTnC

38 uscle-specific enhancer-promoter of the slow/cardiac troponin C (cTnC) gene contains five protein bin

39 Cardiac troponin C (cTnC) is the Ca(2+)-binding componen

40 Human cardiac troponin C (cTnC) is the Ca(2+)-sensing componen

41 Cardiac troponin C (cTnC) is the calcium-dependent switc

42 Cardiac troponin C (cTnC) is the regulatory protein that

43 These changes include the opening of the cardiac troponin C (cTnC) N-domain, the change of second

44 e regulatory N-terminal domain in Ca2+-bound cardiac troponin C (cTnC) presents a much different bind

45 2+) to the N-terminal regulatory lobe of the cardiac troponin C (cTnC) subunit in the troponin comple

46 ique 31-residue N-terminal region that binds cardiac troponin C (cTnC) to increase the calcium sensit

47 r 85 in the N-terminal hydrophobic region of cardiac troponin C (cTnC) to provide specific sites for

48 in binding site in the cardiac-specific slow/cardiac troponin C (cTnC) transcriptional enhancer and o

49 ein binding site within the cardiac-specific cardiac troponin C (cTnC) transcriptional enhancer.

50 m of 5'-tetramethylrhodamine (5'ATR)-labeled cardiac troponin C (cTnC) was measured to monitor cTnC s

51 The Ca(2+) sensor of the sarcomere, cardiac troponin C (cTnC), plays an important role in re

52 TNNC1, which encodes cardiac troponin C (cTnC), remains elusive as a dilated

53 .I4M substitution in the N-terminal helix of cardiac troponin C (cTnC).

54 33 and levosimendan, are reported to bind to cardiac troponin C (cTnC).

55 ity of the amino-terminal regulatory site of cardiac troponin C (cTnC).

56 f calmodulin (CaM) and the fourth EF-hand of cardiac troponin C (cTnC).

57 llosterically mediated, changes to the cTnC (cardiac troponin C)-cTnI (cardiac troponin I) interface

58 Coincident S-nitrosylation of cardiac troponin C decreases myocardial sensitivity to C

59 Fluorescence emission from cardiac troponin C(F20W/N51C)(AEDANS) complexed to cardi

60 The regulatory N-domains of cardiac troponin C(F27W) and its mutants were also able

61 calcium affinity of the regulatory domain of cardiac troponin C(F27W) approximately 2.1-15.2-fold.

62 To sensitize the N-domain of cardiac troponin C(F27W) to calcium, we individually sub

63 ociation and dissociation of the N-domain of cardiac troponin C(F27W).

64 t position 27 with Trp, making a fluorescent cardiac troponin C(F27W).

65 binding affinity of the regulatory domain of cardiac troponin C for cardiac troponin I(129-166) and p

66 oximately 8-fold decrease in the affinity of cardiac troponin C for the regulatory region of cardiac

67 mutations minimally affected the affinity of cardiac troponin C for the regulatory region of cardiac

68 Cardiac troponin C has been postulated to be a potential

69 (2+) affinity of hypertrophic cardiomyopathy-cardiac troponin C (HCM-cTnC) mutants.

70 binding characteristics of human recombinant cardiac troponin C in in vitro conditions.

71 The role of the C-domain sites of cardiac troponin C in the modulation of the calcium sign

72 th mixtures of functional cardiac and mutant cardiac troponin C insensitive to calcium and permanentl

73 eins on the kinetics of Ca(2+) exchange with cardiac troponin C is essential to elucidating the Ca(2+

74 Here we show a large decrease in cardiac troponin C linker flexibility, corresponding to

75 The design of calcium-sensitizing cardiac troponin C mutants presented in this work enhanc

76 ac thin-filament activation, the N-domain of cardiac troponin C (N-cTnC) binds to Ca(2+) and interact

77 hy-linked mutations A8V, E134D, and D145E in cardiac troponin C on the properties of the C-domain sit

78 omplex correlate with partial opening of the cardiac troponin C regulatory domain previously demonstr

79 interaction of troponin I(129-166) with the cardiac troponin C regulatory domain.

80 pecific amino-terminus of troponin I and the cardiac troponin C regulatory domain.

81 interacts with and induces an opening of the cardiac troponin C regulatory domain.

82 ation, in general, and for phospholamban and cardiac troponin C S-nitrosylation, in particular, in be

83 e proton/nitrogen chemical shift analysis of cardiac troponin C showed that, in the presence of cTnI-

84 dge binding cooperatively induced changes in cardiac troponin C structure, as measured by dichroism o

85 ndling proteins, including phospholamban and cardiac troponin C, thereby playing an essential and pre

86 The C terminal of cardiac troponin C (TnC) has two Ca2+-Mg2+ sites which e

87 Cardiac troponin C (TnC) is composed of two globular dom

88 The ability of calcium-bound human cardiac troponin C to neutralize the inhibition of K206I

89 k filament proteins influence the ability of cardiac troponin C to sense and respond to Ca(2+).

90 tions due to troponin I(129-166) binding the cardiac troponin C/troponin I(1-80) complex correlate wi

91 ) for cardiac troponin I(129-166) binding to cardiac troponin C/troponin I(1-80) was 43.3 +/- 3.2 mic

92 eronuclear magnetic resonance studies of the cardiac troponin C/troponin I(1-80)/troponin I(129-166)

93 t both the NH2- and COOH-terminal domains of cardiac troponin C tumble with similar correlation times

94 entify conformational and dynamic changes in cardiac troponin C upon binding a phosphomimetic troponi

95 TNNC1 (cTnC [cardiac troponin C]) variants are implicated in DCM, hyp

96 pecific regulatory regions of Aldolase A and Cardiac troponin C via Trex/MEF3 sites.

97 latory N-terminal domain (N-domain) of human cardiac troponin C, we substituted Phe at position 27 wi

98 mational changes in the regulatory domain of cardiac troponin C were monitored in complexes with trop

99 C and replacement with functional and mutant cardiac troponin C were used to evaluate the relationshi

100 n the regulatory domain of calcium-saturated cardiac troponin C when bound to the NH2-terminal domain

101 ponin I (corresponding to residues 34-71) to cardiac troponin C with the D145E mutation was not able