ライフサイエンスコーパス: cargo

1 arbon stress as well as potential autophagic cargo.

2 ase inw the transport rate of small-molecule cargo.

3 plexes (polyplexes) and the release of their cargo.

4 tact between the carrier and the hitchhiking cargo.

5 cles for the dynamic transport of functional cargo.

6 lar delivery of molecular and macromolecular cargo.

7 whether they act simultaneously on the same cargo.

8 DP52 recruits the ULK1 complex to autophagic cargo.

9 n of an autophagosome that degrades cellular cargo.

10 containing TNT that are long and carry HIV-1 cargo.

11 ing novel CPPs for transcellular delivery of cargo.

12 cond temperature, could grip and transport a cargo.

13 ategy modulates the IgG response against AAV cargos.

14 d on expression of Grasp55-dependent protein cargos.

15 the C-terminal PDZ-binding motifs of protein cargos.

16 pter linking kinesin-1 motor and presynaptic cargos.

17 ulates transport of Golgi apparatus-resident cargos.

18 riched in RNA-binding proteins (RBPs) and EV cargoes.

19 isolate EMVs and begin to characterize their cargoes.

20 BSome, which mediates trafficking of ciliary cargoes.

21 , termed SNX-BAR-binding motif (SBM), in the cargoes.

22 ynein to its general cofactor, dynactin, and cargoes.

23 les for the transport of biologically active cargo across the cell membrane.

24 rtantly, Zn(II)-mediated hydrolysis triggers cargo activation.

25 The complex acts as a cargo adapter that recognizes signaling proteins such as

26 dynein motors assembled with dynactin and a cargo adaptor.

27 aryopherins, including importin beta and its cargo adaptors, have been shown to co-precipitate with t

28 olocalized, or dissociated, state of the dye cargo after exposure to human biologic fluids.

29 rans-Golgi network (TGN) where their soluble cargo aggregates to form a dense core, but the mechanism

30 ven molecular motor that transports cellular cargo along microtubules.

31 responsible for most retrograde transport of cargoes along microtubules in eukaryotic cells, includin

32 en characterized that interact both with the cargo and components of the autophagic machinery, thus p

33 ation for the triggered delivery of internal cargo and facilitated transport of external agents to th

34 EVs contain a defined protein and small RNA cargo and have roles in intercellular communication amon

35 e markedly increased brain uptake of protein cargo and increased brain concentrations of the Abeta bi

36 1BP1's ability to cluster FIP200 around NBR1 cargo and induce local autophagosome formation enforces

37 was revealed nonetheless by their organellar cargo and the grooves they formed indenting MCs, which w

38 emic exposure of nanocarrier delivered toxic cargoes and increasing nanoparticle concentrations in av

39 c link between reduced transport of synaptic cargos and impaired maintenance of synaptic transmission

40 ng factors into the tegument compartment as 'cargoes', and should inform future anti-EBV strategies.

41 of the dated units characterize the crystal cargo, and Advanced-InSAR analysis highlights active def

42 mes mediate enzymatic degradation of vesicle cargo, and also serve as functional platforms for mTORC1

43 Thus, specific cargoes are internalized by ROCK2-mediated activation of

44 mbly of motor-adaptor complexes ensures that cargoes are loaded at their origin and unloaded at their

45 , previously developed regulatable secretory cargoes are often tricky to use or specific for a single

46 nrich EVs with the most effective anticancer cargo as a promising new anticancer approach.

47 lay a variety of functional protein domains (cargo) at defined positions around its periphery.

48 Hence, hnRNPA2B1 carries its cargo, at most, to the site of exosome assembly, but it

49 xpression platforms, can employ a variety of cargo attachment strategies and can be used for applicat

50 ls revealing distinct differences in protein cargo between cancer and normal vesicles.

51 r, which regulates retrograde trafficking of cargo between the Golgi apparatus and the endoplasmic re

52 ric class of transport vesicle that transfer cargo between these varied locations.

53 hat substituting delivery vans with electric cargo bikes can lead to a GHG emission reduction of 26%

54 that both pH and redox environments regulate cargo binding to a hydrophobic site within the cysteine-

55 le mutant inhibits RanGEF without perturbing cargo binding to importin beta and disrupts MI spindle f

56 rprise, this interaction did not require the cargo-binding domain (CBD) of TNPO3, which typically med

57 of the MYO10 motor domain fused to the MYO7B cargo-binding tail domain.

58 chromatin, and forms an inverse gradient to cargo-bound importins.

59 Here, we examine the mechanisms by which a cargo-bound inner coat layer recruits and is organized b

60 modified a regulatable fluorescent secretory cargo by adding a vacuolar targeting signal.

61 dition to the well-known active transport of cargo by motor proteins, many MT-binding proteins seem t

62 including the characterization of their RNA cargos by next generation sequencing (EXO-NGS).

63 on, an imbalance in the traffic of the model cargo Cadherin-2 also reduces neuronal migration.

64 w the functional synergy of the dual-agonist cargo can be tweaked by adjusting the ratio of the two a

65 estigate if modification of specific exosome cargo can rescue reparative activity.

66 the function of TbUnc119 as a myristoylated cargo carrier and support the presence of a conserved LI

67 Only a single homolog to the cargo carrier protein Unc119 has been identified in T. b

68 lead us to propose that microtubules act as cargo carriers to control spatiotemporal protein distrib

69 t cellular processes and structures in polar cargo clustering and provides initial mechanistic insigh

70 IN clusters and the cellular requirements of cargo clustering remain unclear.

71 known to provide a docking site for protein-cargo complexes at the nuclear pore complex (NPC).

72 the overall distribution of activated motor-cargo complexes within cells remains unclear.

73 ifferent transport-competent or -incompetent cargo complexes, and then the permeability barrier prope

74 ssessments of nuclear transporters and their cargoes confirm these observations, revealing disrupted

75 results contribute to what we know about the cargo contained within or excluded from cancer cell-deri

76 Here, we analyzed the protein cargo contained within these vesicles using mass spectro

77 bled the design of systems whereby different cargoes could be moved between cages using acid and base

78 nated aggregates due to decreased autophagic cargo degradation.

79 Second, how are cargoes delivered from PVE compartments to the vacuole?

80 acellular vesicle biogenesis, transport, and cargo delivery and function is needed for successful cli

81 ntial roles in regulating vesicle fusion and cargo delivery at the division site during cytokinesis.

82 (8)) participate in cargo delivery from exosomes of hepatitis A virus (HAV)-

83 Quantification of cargo delivery to and/or accumulation within endolysosom

84 rating this strategy's applicability for the cargo delivery.

85 LLR) and polyarginine tail length (R(7)) for cargo delivery.

86 multicompartment motors, loaded with a model cargo, demonstrate a homogeneous cargo distribution alon

87 between nanocarrier and their small molecule cargos developed here will aid in broader efforts to dec

88 ith a model cargo, demonstrate a homogeneous cargo distribution along with approximately four-fold en

89 Overexpression of the Klp64D cargo domain also results in dominant-negative wing notc

90 icantly extends the capabilities in targeted cargo/drug delivery, environmental remediation, and othe

91 ent in the transport of multiple cytoplasmic cargo (e.g., proteins, protein aggregates, lipid droplet

92 ric cylinder hydrogels loaded with different cargoes (e.g., model protein with different fluorophores

93 have the ability to deliver neuroprotective cargo effectively to the brain.

94 olds featuring a higher affinity for the DNA cargo enabled chemically programmed communication betwee

95 osahedral-like particles with a dense enzyme cargo encapsulated within a proteinaceous shell.

96 enzyme activation, shell self-assembly, and cargo encapsulation to construct a robust nanoreactor th

97 roautophagy, involves membrane mobilisation, cargo engulfment and trafficking of the newly formed aut

98 ives in part from the biophysical process of cargo enrichment into a constrained spherical membrane-b

99 While previous studies suggested that cargo ensures its own internalization by regulating eith

100 depends on the motor protein MYO15A and its cargo EPS8.

101 ependent on the motor protein MYO15A and its cargo EPS8.

102 terred and through the exploitation of their cargo, EVs may provide an effective mean of drug deliver

103 m) that efficiently encapsulate nucleic acid cargo, exhibit sustained release of payload, and can sta

104 tor, the number of binding interfaces to the cargo factor, and more.

105 ding affinity of the targeting signal to the cargo factor, the number of binding interfaces to the ca

106 secretory pathway, optimization of antigenic cargo, final release to the cell surface, and engagement

107 elective autophagy receptors (SARs) mark the cargo for degradation and, in yeast, recruit Atg11, the

108 the vacuole or lysosome, thereby delivering cargo for degradation.

109 b11 vesicle motility to deliver an essential cargo for lumen formation, CFTR (cystic fibrosis transme

110 ive autophagy receptors to target type-A ARR cargos for autophagic degradation, demonstrating modulat

111 perform various functions, such as engaging cargos for transport or engaging peripheral actin to sta

112 -cellular barriers, protect the nucleic acid cargo from degradation with stability over a range of te

113 Because extracellular vesicles derive their cargo from the contents of the cells that produce them,

114 1) is a nuclear import protein that shuttles cargo from the cytoplasm to the nucleus.

115 TANGO1-mediated transfer of bulky secretory cargos from the ER to the ERGIC/Golgi via a tunnel.

116 In fact, the EMV cargo genes exhibited normal expression patterns after g

117 lows associated desirable genetic material ("cargo genes") to increase in frequency.

118 Since Coa is able to protect cargo GF and maintain its long-term bioactivity, it is s

119 eted delivery of nanomaterials with chemical cargoes guided by biorecognition motifs has a broad rang

120 nd glial function via their microRNA (miRNA) cargo has positioned them as a novel and effective metho

121 Endosomally entrapped cargo, however, can have very low escape efficiency, wit

122 Further analysis of the localization of SG cargoes immediately after budding from the TGN revealed

123 tify the mechanism of action, analysis of EV cargo implicated miRNA (miR-124) as a potential candidat

124 s reveal key molecular determinants of large cargo import in cells.

125 e rotational and translational motion of the cargo in a 3D cell cytoskeleton was obtained.

126 pathway for S. aureus to transport protected cargo in a concentrated form to host cells during infect

127 I)-targeting mechanism enriches the inactive cargo in beta-cells as compared to other pancreatic cell

128 ejected dysfunctional mitochondria and other cargo in dedicated membranous particles reminiscent of n

129 concentration of any associated therapeutic cargo in the placental tissue.

130 form for selective release of small-molecule cargoes in beta-cells over other islet cells ex vivo or

131 n beta, importin alpha/beta, and transportin cargoes in permeabilized mouse neurons and HeLa cells, i

132 fficient transport of many dynein-associated cargos in cells.

133 known endocytosis mechanism for HSPG-binding cargos in general, which requires forces generated by MY

134 argo receptors, but the identity of putative cargos in plants is still elusive.

135 at a particle designed to discharge thrombin cargos in response to an external stimulus, such as H(2)

136 tem for intercellular transfer of biological cargo, including RNA, that plays a pivotal role in physi

137 escribed here, with exosomes carrying unique cargos, including the GTPase Rab11, generated in Rab11-p

138 cytosis (CME) represents the major route for cargo internalisation from the cell surface.

139 the biochemical pathway for CI-M6PR-mediated cargo internalization in cell lines, and uncover the exo

140 g can detect earlier roles of EAPs preceding cargo internalization; however, this approach has been l

141 oited for drug delivery to carry impermeable cargo into cells, but their inherent toxicity results in

142 e and human TfR1 was used to shuttle protein cargo into the brain.

143 anism by which ATR signals that its cofactor cargo is ready (AdoCbl) or not [cob(II)alamin] for trans

144 ng endosomes and Golgi trafficking where the cargo is released intracellularly.

145 The Er1(F/-) EV cargo is taken up by recipient cells leading to an incre

146 ved Golgi localization of O-glycan-deficient cargos is due to their slow Golgi export.

147 As LE-to-ER transport of cellular cargos is unclear, our results have broad implications f

148 , lysosome-mediated degradation of autophagy cargoes, is compromised in cystinosis.

149 For some therapeutic cargoes, it is essential to maintain contact with the bl

150 on the plasma membrane to reshape it into a cargo-laden vesicle.

151 in/Rvs (BAR) proteins can directly recognize cargoes like cation-independent mannose 6-phosphate rece

152 At the peroxisomal membrane, the cargo-loaded receptor interacts with the docking protein

153 and FMR1, play a significant role in exosome cargo loading and enhanced secretion during cellular inf

154 nce, the LC3-conjugation pathway controls EV cargo loading and secretion.

155 ion of pro-IL-1beta, serving as a signal for cargo loading into secretory vesicles.

156 itude larger than small molecules, such that cargo loading is better described by co-assembly process

157 pport a model in which R-DPRs interfere with cargo loading on karyopherins.

158 atical modeling analyses suggest that active cargo loading reduces non-specific encapsulation of cell

159 PRs interact with importin beta, disrupt its cargo loading, and inhibit nuclear import of importin be

160 riphery and induces selective exosomal miRNA cargo loading.

161 The second sequence uncages the carrier's cargo locally to achieve high target specificity without

162 EVs), exosomes and microvesicles, containing cargo mediators, such as proteins and RNAs, play a key r

163 ng de novo expression of proteins encoded by cargo messenger RNA (mRNA).

164 es in postsynthetic loading of more than one cargo molecule.

165 acellular vesicles (EVs) containing specific cargo molecules from the cell of origin are naturally se

166 rosinase-mediated oxidation of phenol-tagged cargo molecules is a particularly convenient method of g

167 reveals that SMO, and likely also other GPCR cargoes, must release their amphipathic helix 8 from the

168 idence shows that a number of intracellular "cargos" navigate the cytoplasm by hitchhiking on motor-d

169 ER leakage is influenced by vesicle size and cargo occupancy: overexpressing an inert cargo protein o

170 The recent discovery that the metabolite cargo of dead and dying cells ingested through efferocyt

171 allows each unit to be loaded with biologic cargo of different compositions, thus enabling controlla

172 is particularly strong evidence that the RNA cargo of extracellular vesicles can alter recipient cell

173 The cargo of holoBLG is decisive in preventing allergy in vi

174 The fact that TGs and SEs are the typical cargo of lipid droplets suggests that these organelles c

175 Overall, these data suggest that the miRNA cargo of plasma CD31(+) EVs is largely affected by T2DM

176 r crowding in concert with the transmembrane cargo on the membrane induce membrane deformation and fa

177 ach can be used to append nearly any kind of cargo onto serine, generating a stable, benign, and hydr

178 Given their ability to transport functional cargos originating from the source cells to target cells

179 ssembly critical for sorting and trafficking cargo out of the endosome.

180 ly, the bulk of two studied transmembrane SG cargoes (phogrin and VMAT2) does not sort directly onto

181 regulation of sEV secretion and PTX3 protein cargo primes the premetastatic niche and suggests that i

182 ustering of retromer-bound integral membrane cargo prior to its packaging into a nascent transport ca

183 The ppH protocol makes use of cargo protein (e.g., the transferrin receptor) coupled t

184 and cargo occupancy: overexpressing an inert cargo protein or reducing vesicle size restores sorting

185 ced by the accumulation of the autophagosome cargo protein p62/SQSTM1, and a poorly inducible autopha

186 Binding of the cargo protein to PCAT1 variants devoid of the PEP domain

187 orly understood how SNX-BARs select specific cargo proteins and whether they recognize additional lig

188 minants of the interaction between PCATs and cargo proteins are poorly understood, yet this interacti

189 etwork (TGN), likely aiding the transport of cargo proteins from the TGN for proper location; but EPS

190 siae), EVs function as carriers to transport cargo proteins into the periplasm for storage during glu

191 nization of the eukaryotic cell and delivers cargo proteins to their subcellular destinations, such a

192 ex5 cargo proteins were outcompeted, several cargo proteins were not affected, implying that they hav

193 We found that while most known Pex5 cargo proteins were outcompeted, several cargo proteins

194 h SCeVD (n = 16) for ELISA quantification of cargo proteins.

195 hway in intact NPCs in cells: that is, inert cargoes ranging from small proteins to large capsids wer

196 endent mechanism that involves the autophagy cargo receptor NBR1.

197 The plant selective autophagy cargo receptor neighbour of breast cancer 1 gene (NBR1)

198 inhibiting self-interaction of the autophagy cargo receptor p62/SQSTM1, impeding p62 autophagy flux.

199 und that inducing internalization of a SNX17 cargo receptor, low-density lipoprotein receptor-related

200 pon preventing accumulation of the autophagy cargo receptor, Neighbor to BRCA1 (NBR1).

201 that exits the ER with the aid of the Erv29 cargo receptor, which is homologous to mammalian Surf4.

202 a its C-terminal domain to impair docking of cargo-receptor (karyopherin/importin) complex and disrup

203 e proteins have been proposed to function as cargo receptors, but the identity of putative cargos in

204 ive forms of macroautophagy, specifically on cargo recognition by autophagy receptor proteins p62 and

205 Cargo recognition is mediated by components of the coat

206 We propose that cargo recruitment into vesicles creates a crowded lumen

207 ects of LRRK2 kinase inhibition in promoting cargo recycling.

208 O) display impaired transport of presynaptic cargos, reduced synapse density and active zones, and al

209 escue data indicate that both AR2 and Klp64D cargo regions are required for the function of Arm and K

210 stress and leading to near-complete vesicle cargo release in sub-seconds.

211 e form is disabled in terms of light-induced cargo release, however, bioorthogonal transformation of

212 pe and cysteine for stability and controlled cargo release.

213 issolves, promoting the autonomous localized cargo release.

214 t ubiquitylation alone is not sufficient for cargo release.

215 targeted affinity ligands and biotherapeutic cargo remain a largely unexplored area, despite obvious

216 cytosol arrival and disassembly, and suggest cargo remodeling as a novel function of dynein adaptors.

217 albumin in transport of endogenous nutrient cargos required for cellular growth and not just a sugge

218 ic database of soluble proteins and exosomal cargo SASP factors originating from multiple senescence

219 viding insights into the mechanisms of large cargo secretion that may be relevant for COPII-related d

220 Cargo selectivity and activation of processive motility

221 n through a checkpoint in the pathway as the cargo-sensitive step.

222 We further show that, by monitoring two cargoes simultaneously, it becomes possible to visualize

223 ntrolling the sorting of regulated secretory cargoes (soluble and transmembrane) away from constituti

224 ESCRT-0, -I, and -II presumably involved in cargo sorting and ESCRT-III in membrane deformation and

225 ates exosome biogenesis and exosomal protein cargo sorting through the control of cholesterol content

226 by a Ca2+-mediated process that involves the cargo-sorting protein Cab45.

227 s shared by golgin-97 and GCC88, and various cargoes specific to individual golgins.

228 The cargo-specific removal of organelles via selective autop

229 vesicle generation at the TGN with different cargo specificity and destination.

230 nduce local autophagosome formation enforces cargo specificity and replaces the requirement for lipid

231 ical systems can act as transporters to move cargo such as hydrogel alginate capsules containing livi

232 is reduced insulin secretion, and mature DCV cargoes such as insulin and carboxypeptidase E (CPE) acc

233 that cross the NPC, even very large (>15 nm) cargoes such as pathogens, mRNAs and pre-ribosomes can p

234 eptide can be used to deliver small molecule cargos such as contrast agents to permit future in vivo

235 Unique biomolecular cargos such as RNA and protein are loaded in these vesic

236 nisms regulating the degradation of specific cargos, such as dysfunctional organelles and protein agg

237 D, but only the second AR (AR2) binds to the cargo/tail domain of Klp64D.

238 alpha-Syn PFFs and other polycation-bearing cargos that enter cells via HSPGs.

239 tophagosome selectively around the cytosolic cargo, that is, a protein aggregate, a mitochondrion, or

240 In certain scenarios, depending on loaded cargos, the vesicles have spatially distinct destination

241 For these cargoes, there is little quantitative understanding of t

242 It is not clear what cargo these vesicles contain and how they are released.

243 They contain specific cargo, they have certain R-SNAREs for fusion, and they a

244 ity ligand to deliver a prototypical surface cargo, thrombomodulin (TM), using one-to-one protein con

245 imit the dynein-mediated stripping of corona cargoes through a direct interaction with Nde1.

246 ization, and translocate phagosomes or other cargo to appropriate cellular locations.

247 f AAV2.retro to deliver disease-related gene cargo to biologically-relevant NHP brain circuits by pac

248 the ability of such carriers to target their cargo to cancer cells is crucial.

249 resulted in the efficient delivery of (19)F cargo to EpiSCs and enabled their visualization by (19)F

250 SL-1 enhanced the trafficking of phagocytic cargo to lysosomes.

251 modulate the host extracellular vesicle (EV) cargo to manipulate the deubiquitination machinery of th

252 deliver their biologically active molecular cargo to recipient cells.

253 hange upon heating, causing the cage and its cargo to reversibly transfer between aqueous and organic

254 unique mechanism, potentially allowing other cargo to simultaneously bind TNPO3.IMPORTANCE RSV Gag nu

255 the lymph node reticular network, delivering cargo to specific cells in the lymph node cortex and par

256 as myosin XI, associate with their secretory cargo to support the ubiquitous processes of polarised g

257 r transferrin-coated vesicles for delivering cargo to the mouse brain.

258 By tethering the cargo to the UBLs present on the forming autophagosome,

259 n with relocalisation of transporters and/or cargoes to ataxin-1[85Q] nuclear bodies.

260 ficiency and specificity of QD with chemical cargoes to chloroplasts in plant cells in vivo (74.6 +/-

261 ) networks is exploited by motors to deliver cargoes to specific intracellular destinations and is th

262 croautophagy (autophagy) targets cytoplasmic cargoes to the lysosome for degradation.

263 ction requires molecular motors to transport cargoes to their correct intracellular locations.

264 ated transport system that delivers selected cargos to specific synapses.

265 rent delivery and sequential release of dual cargos toward combinatorial therapy.

266 t drives the transport of many intracellular cargoes towards the minus end of microtubules (MTs).

267 Nuclear pore complexes (NPCs) regulate all cargo traffic across the nuclear envelope.

268 , pH homeostasis in recycling endosomes, and cargo trafficking, and they also triggered apoptosis.

269 endrite development and facilitate polarized cargo trafficking; however, the mechanism that regulates

270 al cell-cell labeling, interaction-dependent cargo transfer, and the identification of higher order c

271 ant roles in cell-cell communication through cargo transfer.

272 s the surfactants that the droplet needs for cargo transport and the artificial system provides the t

273 exact mechanisms by which TBC1D23 regulates cargo transport are poorly understood.

274 Delayed cargo transport conditions correlate strongly with a pro

275 g a region of the TGN devoted to specialized cargo transport in general rather than being specific fo

276 It plays diverse roles in cargo transport including anterograde (base to tip) traf

277 Also, both motor velocities as well as cargo transport speeds were visibly increased in neurons

278 ecific kinesin-2 implicated in intracellular cargo transport.

279 e explained by a cisternal-specific delay in cargo transport.

280 mplications for illuminating inter-organelle cargo transport.

281 Processive molecular motors enable cargo transportation by assembling into dimers capable o

282 xt to a curvilinear track, e.g., a cytosolic cargo transported by motor proteins moving along a micro

283 It has long been assumed that cargo triggers local CME site assembly in Saccharomyces

284 tasis and relies on motor complexes bound to cargoes via specific adaptors.

285 (ICLs, DiR, DiD, DiI) as a model lipophilic cargo, via different carriers.

286 nd demonstrated that over 99% of the protein cargo was subsequently incorporated into HCMV virions du

287 f a ligand to generate a nearly synchronized cargo wave.

288 ood which kinesins are present on particular cargos, what their contributions are and whether they ac

289 elease of intra-lymph-mobile small-molecular cargo, which can reach vastly more immune cells througho

290 The DNC can host nanoscale cargoes, which allows for the integration with functiona

291 icles (autophagosomes) sequester cytoplasmic cargos, which are subsequently delivered to the lysosome

292 cellular vesicles carrying diverse molecular cargos, which can modulate recipient cell behaviour.

293 by endosomal entrapment of delivered protein cargo with concomitantly inefficient access to the cytos

294 The cleavage releases the cargo with the N-terminal linker amino acid from the pep

295 es like ours are imperative to identify CRM1 cargoes with real pathogenic potential.

296 onent cytoplasmic dynein transports cellular cargoes with the help of another multi-component complex

297 munication structures transporting different cargos with potential implications in therapy resistance

298 iculum (ER) exit sites, where it binds bulky cargo within the ER lumen and recruits membranes from th

299 The clustering of PIN polar cargoes within the plasma membrane has been proposed to

300 cteristics for engulfing NPs and other large cargo, yet its molecular machinery and involvement in NP