ライフサイエンスコーパス: caveolin-2

1 response; Cavin 2) and CAV1/CAV2 (Caveolin 1/Caveolin 2).

2 ng domains of caveolins 1 and 3 but not with caveolin 2.

3 with increased expression of caveolin 1 and caveolin 2.

4 n-1 expression on the behavior of endogenous caveolin-2.

5 of caveolin-1 but contain normal amounts of caveolin-2.

6 cell-type and tissue-specific expression of caveolin-2.

7 caveolin gene has been identified and termed caveolin-2.

8 rom a complex of the proteins caveolin-1 and caveolin-2.

9 antly decreased expression of caveolin-1 and caveolin-2.

10 orming gene 1, Polo-like kinase 1 (PLK1) and Caveolin-2.

11 ly up-regulates the expression of endogenous caveolin-2.

12 They were cofractionated with caveolin-2, a lipid-raft-associated intracellular membra

13 Here, we demonstrate that host deficiency in caveolin-2, a member of caveolin protein family, increas

14 colocalizes with caveolin-1, indicating that caveolin-2 also localizes to caveolae.

15 ysis of 6538 genes revealed that caveolin 1, caveolin 2, amphiregulin, and melanoma growth stimulator

16 e cell population (>95%) that also expressed caveolin 2 and GLUT4 with very low levels of the PDGF re

17 failed to interact with full-length forms of caveolin-2 and caveolin-3.

18 oid (FRT) cells, which express low levels of caveolin-2 and no caveolin-1, caveolin-2 localizes exclu

19 co-immunoprecipitation experiments show that caveolin-2 and Ras-GAP are constitutively associated in

20 Caveolins 2 and 3 were also detected in purified caveoli

21 latively low levels of the insulin receptor, caveolin 2, and GLUT4.

22 Notably, caveolin-1, caveolin-2, and annexin A2, which are proteins associate

23 e commonly down-regulated genes, caveolin-1, caveolin-2, and GDF10/BMP3b, were chosen for further stu

24 marily stimulated Akt phosphorylation in the caveolin 2- and GLUT4-negative cell population.

25 stimulated the phosphorylation of Akt in the caveolin 2- and GLUT4-positive cells, whereas PDGF prima

26 Two of three caveolin-2 antibodies show little basal staining, but st

27 d raft-dependent tyrosine phosphorylation of caveolin-2 appeared to be a critical regulator of P. aer

28 mRNAs for both caveolin-1 and caveolin-2 are most abundantly expressed in white adipos

29 In MDCK cells, both caveolin-1 and caveolin-2 associated with low density Triton-insoluble

30 g caveolins, we show that phosphorylation of caveolin-2 at serines 23 and 36 can be differentially re

31 reatment led to the exclusion of caveolin-1, caveolin-2, beta1-adrenergic receptors (beta1-AR), beta2

32 throleukemic cell line, K562, that expresses caveolin-2 but fails to express detectable levels of cav

33 ion of lung epithelial cells is dependent on caveolin-2 but not caveolin-1.

34 noclonal antibody probe that recognizes only caveolin-2, but not caveolins-1 and -3.

35 ectly address the functional significance of caveolin-2 by genetically targeting the caveolin-2 locus

36 mains, where it decreases phosphorylation of caveolin-2 by inactivating c-Src.

37 Phosphorylation of caveolin-2 by Src family kinases is an essential event f

38 Caveolin-2 (Cav-2), a member of caveolin protein family,

39 tric inhibitory polypeptide receptor (GIPR), caveolin 2 (CAV2), and peptidase D (PEPD) (P-interaction

40 composed primarily of caveolin-1 (Cav1) and caveolin-2 (Cav2) proteins.

41 Caveolin-2 colocalizes with caveolin-1, indicating that

42 esults in Group V PLA2 being associated with caveolin-2-containing granules close to the perinuclear

43 sing the anti-tumor immune response and that caveolin-2 could be a potential target for cancer immuno

44 reduced in Cav-1-null mice, we conclude that caveolin-2 deficiency is the clear culprit in this lung

45 ith the latter, transfer and co-injection of caveolin-2 deficient bone marrow (origin of TAMs) suppre

46 ltrating CD8 T cells and tumor regression in caveolin-2 deficient mice, suggesting that an early incr

47 upregulation of serine 23 phosphorylation of caveolin-2 depends on caveolin-1-driven targeting to pla

48 In FRT cells, endogenous or overexpressed caveolin-2 did not associate with low density Triton ins

49 ar distribution, endogenous or overexpressed caveolin-2 did not promote the assembly of caveolae; rat

50 current, whereas antibodies to caveolin-1 or caveolin-2 did not.

51 However, caveolin-1 and caveolin-2 differ in their functional interactions with

52 bodies directed against either caveolin-1 or caveolin-2 directly show that these molecules form a sta

53 l signaling genes including RhoB, PTGF-beta, caveolin-2, Egr-1, myosin 1B, and EHM2.

54 Peptides from caveolin-2 exhibited no effect, whereas caveolin-1 and -

55 our data suggest that lung cancer cells use caveolin-2 expressed in bone marrow-derived cell types i

56 We therefore examined caveolin-1 and caveolin-2 expression in resident and thioglycollate-eli

57 As caveolin-2 expression is also severely reduced in Cav-1-

58 nerve growth factor (NGF) treatment, whereas caveolin-2 expression is transiently up-regulated early

59 -localization, it appears that regulation of caveolin-2 expression occurs independently of the expres

60 show a approximately 95% down-regulation in caveolin-2 expression; these cells also do not express c

61 nds, whereas the corresponding domain within caveolin-2 fails to recognize these ligands under the sa

62 udomonas co-opts the endocytic properties of caveolin-2 for invasion, there also exists in these cell

63 st, in the absence of caveolin-1 expression, caveolin-2 forms low molecular mass oligomers that are r

64 ular machinery to induce the dissociation of caveolin-2 from caveolin-1 oligomers.

65 -expression is required for the transport of caveolin-2 from the Golgi complex to the plasma membrane

66 The CAV1/CAV2 (caveolin 1 and caveolin 2) genomic region previously was associated wit

67 signed microarrays showed down-regulation of caveolin 2, GNB4, and lipase A in AD Rac1b-positive/p75(

68 t notably adipocytes and endothelial cells), caveolin-2 has traditionally been considered the dispens

69 c-Src, NCK, and Ras-GAP, that interact with caveolin-2 in a phosphorylation-dependent manner.

70 Conversely, transient expression of caveolin-2 in CHO cells is sufficient to up-regulate end

71 phenotypes, indicating a selective role for caveolin-2 in lung function.

72 show for the first time a specific role for caveolin-2 in mammalian physiology independent of caveol

73 ible involvement of serine 36-phosphorylated caveolin-2 in modulating mitosis.

74 ression of caveolin-1 or caveolin-3, but not caveolin-2, in Cav-1 null cells complements this phenoty

75 lates caveolin-1 protein expression, but not caveolin-2, in cultured NIH 3T3 and Rat 1 cells.

76 ovel mechanism, via which host deficiency in caveolin-2 inhibits tumor growth.

77 vesicles, but does not affect trafficking of caveolin-2, interaction with caveolin-1 or its biophysic

78 At the level of the ER, caveolin-2 interacts with caveolin-1 to form a high mole

79 Caveolin-2 is a member of the caveolin gene family with

80 Thus, the phosphorylation of caveolin-2 is a novel mechanism to regulate the dynamics

81 Here, we present the first evidence that caveolin-2 is a phosphoprotein.

82 It is believed that caveolin-2 is an "accessory protein" that functions in c

83 Caveolin-2 is an accessory molecule and the binding part

84 Although caveolin-2 is coexpressed and heterooligomerizes with ca

85 ine residues and that the phosphorylation of caveolin-2 is necessary for its actions as a positive re

86 However, caveolin-2 is not required for caveolae formation, imply

87 More importantly, we show that caveolin-2 is phosphorylated in vivo at two serine resid

88 Caveolin-2 is the least well studied member of the caveo

89 ient in all three caveolin gene products, as caveolin-2 is unstable in the absence of caveolin-1.

90 Interestingly, caveolin-2 is up-regulated in response to the mechanical

91 ation event was selective for caveolin-1, as caveolin-2 levels remained constant under these conditio

92 Ultrastructurally, this pattern of caveolin-2 localization corresponds to caveolae membrane

93 nstitutively express both caveolin-1 and -2, caveolin-2 localized to both the Golgi complex and to th

94 low levels of caveolin-2 and no caveolin-1, caveolin-2 localizes exclusively to the Golgi complex bu

95 e of caveolin-2 by genetically targeting the caveolin-2 locus (Cav-2) in mice.

96 ence of caveolin-1, serine 23-phosphorylated caveolin-2 mostly localizes to plasma membrane, while se

97 Caveolin-2 mRNA and protein expression were unchanged ov

98 Northern blot analysis reveals that caveolin-2 mRNA levels remain constant under these condi

99 Caveolin-2, on the other hand, is expressed in all cells

100 noviral transduction of either caveolin-1 or caveolin-2 or both isoforms into cells lacking both cave

101 These data support a role for caveolin 1, caveolin 2, or both in POAG and suggest that the caveoli

102 We also found that caveolin-1, caveolin-2, or Cav-1 S80E formed immunoprecipitable comp

103 of caveolin-1 in endothelial cells decreases caveolin-2 phosphorylation at serine 23 but not at serin

104 ceptor in the insulin receptor-, GLUT4-, and caveolin 2-positive cells resulted in the ability of PDG

105 lacking both caveolins, we demonstrate that caveolin-2 positively regulates caveolin-1-dependent cav

106 s revealed that serine 23 phosphorylation of caveolin-2 preferably occurs in detergent-resistant memb

107 sma membrane, while serine 36-phosphorylated caveolin-2 primarily resides in intracellular compartmen

108 This pattern of caveolin-2 protein expression most closely resembles the

109 In the absence of caveolin-2 protein expression, caveolae still form and c

110 embryos reveals the following: (i) a loss of caveolin-2 protein expression; (ii) defects in the endoc

111 cell and tissue types demonstrates that the caveolin-2 protein is most abundantly expressed in endot

112 Also, caveolin-2 protein levels remain unchanged during the di

113 o oncogenic transformation of NIH 3T3 cells, caveolin-2 protein levels remain unchanged.

114 MP2 in NIH3T3 cells decreased caveolin-1 and caveolin-2 protein levels while increasing the surface e

115 ex between caveolins-1 and -2 stabilizes the caveolin-2 protein product and allows caveolin-2 to be t

116 in-1 expression is required to stabilize the caveolin-2 protein product, to mediate the caveolar endo

117 the expression of caveolin-1 stabilizes the caveolin-2 protein.

118 1 and -3, but not the analogous regions from caveolin-2, resulted in inhibition of eNOS, inducible NO

119 rol efflux by HDL(3) leads to caveolin-1 and caveolin-2 secretion to the cell culture medium, a proce

120 tion of the primary phosphorylation sites on caveolin-2, serine 23 and 36, reduces the number of plas

121 Decreased expression of caveolin-2 significantly impaired the ability of P. aeru

122 Cav-1-F92A was measured by stabilization of caveolin-2, sucrose gradient, and electron microscopy.

123 es the caveolin-2 protein product and allows caveolin-2 to be transported from the Golgi complex to t

124 recombinant expression of caveolin-1 allows caveolin-2 to form high molecular mass oligomers that ar

125 onclude that the tyrosine phosphorylation of caveolin-2 (Tyr(P)(19)) may function as a signal that is

126 both induce the tyrosine phosphorylation of caveolin-2 (Tyr(P)(19)).

127 differentiated PC12 cells; up-regulation of caveolin-2 under these conditions is strictly dependent

128 We show that caveolin-2 undergoes Src-induced phosphorylation on tyro

129 The cellular distribution of caveolin 2 was similar to that of caveolin 1.

130 Some overexpressed caveolin-2 was also present in lipid droplets.

131 proximately 20-kDa caveolin-related protein, caveolin-2, was identified through microsequencing of ad

132 in regulating phosphorylation of endogenous caveolin-2, we have used the siRNA approach.

133 presence of PIM1, whereas IL6, cyclin A1 and caveolin 2 were increased.

134 ace, does not co-localize significantly with caveolin-2, which is present primarily in the Golgi comp