ライフサイエンスコーパス: cell isolation

1 samples that avoids the need for mononuclear cell isolation.

2 minimize development of LPS tolerance during cell isolation.

3 using PDL-MSCs requires tooth extraction for cell isolation.

4 xpressed within a tissue of interest without cell isolation.

5 e levels did not significantly change during cell isolation.

6 alone and without cell enrichment or single-cell isolation.

7 ic gene expression profiles without physical cell isolation.

8 d to form peptide-HLA (pHLA)-tetramers for T cell isolation.

9 genome expression analysis, histology, and T-cell isolation.

10 d chromatin-binding proteins in vivo without cell isolation.

11 structures inside a microfluidic channel for cell isolation.

12 llected and used for histologic analysis and cell isolation.

13 ogenic-specific Cre-loxP lineage marking for cell isolation.

14 eld (100%), and viability (94-100%) for rare cell isolation.

15 the cancer target HER2 (ErbB2) for magnetic cell isolation.

16 finity, improving antibody affinity improved cell isolation.

17 that enables cell-specific profiling without cell isolation.

18 Nestin prevents its use for prospective live cell isolation.

19 d as an alternative to antibodies for cancer cell isolation.

20 ny of the limitations of current methods for cell isolation.

21 y robust surface marker profile for CRC stem cell isolation.

22 r the utilization of frozen tissues for stem cell isolation.

23 rming the basis for improved methods of stem cell isolation.

24 stocyst development (62%) and embryonic stem cell isolation (38%) rates were comparable to controls.

25 steps for SOP implementation, namely timely cell isolation after sampling, use of appropriate lysis

26 als with 0.5% (R)-alpha-lipoic acid prior to cell isolation almost completely reversed the age-associ

27 at cord blood was a superior source for Treg-cell isolation and cell line generation compared with ad

28 ells and could be applied to facilitate stem cell isolation and characterization.

29 artial epithelial-to-mesenchymal transition, cell isolation and co-culture studies, high-resolution m

30 hment of novel protocols with optimized stem cell isolation and culture conditions has given rise to

31 imitation have primarily consisted of cancer cell isolation and culture directly from human PC specim

32 e develop a novel program for semi-automated cell isolation and culture equipment to permit complete

33 Establish a robust cardiac stem cell isolation and culture protocol to consistently gene

34 Following cell isolation and culture, 63 +/- 23% of the isolated P

35 However, lack of proper cell isolation and enrichment techniques hinder downstre

36 Advances in hematopoietic stem cell isolation and ex vivo manipulation has kept pace wi

37 Here, we use cultivation-independent single-cell isolation and genome-resolved metagenomics to inves

38 This strategy bypasses the requirement of cell isolation and includes five major steps: (i) constr

39 Bone marrow harvest, cell isolation and infusion were completed by 48 h post-

40 As scFTD-seq decouples on-chip cell isolation and library preparation, we envision it t

41 and human liver and pancreas organoids, from cell isolation and long-term expansion to genetic manipu

42 th a microwell array-patterned face (xy) for cell isolation and lysis.

43 ditioned media still requires time-consuming cell isolation and maintenance and also may contain fact

44 extensive sample preparation, except for the cell isolation and matrix application.

45 Here, using four-way liver cell isolation and parallel comparison of candidate anti

46 two key steps in genetic testing procedures, cell isolation and PCR and promises to be adaptable for

47 s in and out of an 8--9 microL dual-purpose (cell isolation and PCR) glass-silicon microchip.

48 y steps in the analytical procedure, namely, cell isolation and PCR.

49 ease of cells to suspension, prior to single-cell isolation and protein analysis.

50 combining VCS MN with photoactivation-based cell isolation and RNASeq.

51 silicone molds, microfluidic patterning and cell isolation and seeding takes approximately 7 days.

52 g robust organoids, such as those related to cell isolation and seeding, matrix and soluble factor se

53 Approximately 5-12 d after cell isolation and seeding, preparations develop electri

54 ars, the implementation of high-throughput B-cell isolation and sequencing assays and of screening me

55 Using transgene-assisted cell isolation and single cell RNA-sequencing, we charac

56 Primary cell isolation and subculture of PT cells recapitulated

57 human tissues for subsequent post-natal stem cell isolation and tissue regeneration.

58 Cell isolation and transplant studies demonstrated CD62L

59 s 5 kDa smaller than protein from epithelial cells; isolation and sequencing of the monocyte CADTK cD

60 e analytical procedure, contamination during cell isolation, and different levels of vector marking i

61 n and preservation in cardiac myocytes after cell isolation are not well documented.

62 Ordered single-cell isolation arrays allow for high-density microscopic

63 ables automated quantitation and prospective cell isolation as a function of chromatin accessibility,

64 In this study, we show that single-cell isolation biases in microfluidics can be circumvent

65 a more reliable approach to mitigate single-cell isolation biases.

66 olymerization did not have a major effect on cell isolation, but isolation was inhibited by cholester

67 ESCs) has been extensively studied since the cells' isolation, but the necessity for cell-secreted fa

68 application for evaluating the efficiency of cell isolation by collagenases.

69 hair cells, we developed a protocol for hair cell isolation by FACS.

70 onmental microbes via high-throughput single-cell isolation by FACS.

71 Clara cell isolation by flow cytometry sorting is a useful met

72 Direct comparisons with cell isolation by fluorescence-activated cell sorting an

73 Cell isolation can be completed in less than 4 h.

74 les and overview its applications for single cell isolation, cell focusing and sorting, cell washing

75 The protocol describes gill cell isolation, cultured gill epithelium formation, main

76 duced based on three essential capabilities: cell isolation, detection assay and output measurement.

77 ignificantly improve the sensitivity of rare cell isolation devices by increasing the processed whole

78 optimizing the entire workflow from initial cell isolation down to sample preparation, liquid chroma

79 e insights should guide future approaches to cell isolation, either magnetically or using other means

80 generation of embryoid bodies or prospective cell isolation, entails four stages with different cultu

81 these findings for vaccine development and T cell isolation/enumeration are discussed.

82 l differentiation using a three-step system (cell isolation/expansion/differentiation).

83 Cell isolation experiments from psoriatic tissue showed

84 The muscle specimen was shipped to a cell isolation facility where myoblasts were isolated an

85 ies, comprising live-cell imaging and single-cell isolation, followed by freeze-drying, international

86 apture microdissection instrument for single-cell isolation, followed by reverse transcription via Mo

87 Here we introduce SIFT, single-cell isolation following time-lapse imaging, to address

88 ardiography and peripheral blood mononuclear cell isolation for expression profiling and 112 patients

89 ntages of nanoscale reagents in molecule and cell isolations for both research and clinical applicati

90 is study used models allowing the convenient cell isolation from Ccl17(E/+) reporter mice; it also ex

91 Current methods for cell isolation from complex samples are largely dependen

92 Single cell isolation from helminth-infected murine intestines

93 osis and have proved to be very effective in cell isolation from minimally processed primary tissue.

94 tone mark is highly specific to a cell type, cell isolation from tissues is often necessary to genera

95 Population analyses rely on cell isolation from whole organs, and interpretation is

96 Microfluidic systems for affinity-based cell isolation have emerged as a promising approach for

97 by combining in situ hybridization, primary cell isolation, immunoblotting, quantitative RT-PCR, and

98 10 ml whole blood was harvested for cell isolation, immunophenotyping and stimulation.

99 Kupffer cell isolation in IL-6(-/-) females receiving IL-6(+/+)

100 on of zIAA8 is up-regulated within 3 h after cell isolation in inductive medium, indicating that cell

101 ike cell markers and suitable procedures for cell isolation in order that the correct populations are

102 and are widely employed for biomolecule and cell isolations in research laboratories, clinical diagn

103 Advances in cell isolation, in vitro culture techniques, and genetic

104 and could include mRNA contamination during cell isolation, in vivo mRNA paracrine transfer from par

105 l for accurate profiling, yet factors during cell isolation including time ex vivo and temperature in

106 Successful single-cell isolation is a primary step for subsequent chemical

107 enging due to difficulties related to single cell isolation, metabolite detection, and identification

108 Also, this cell isolation method allowed for the time between blood

109 tablish fs-LM as a spatially resolved single-cell isolation method for phenotype-to-genotype mapping.

110 An optimized direct immune cell isolation method prevented loss of up to 90% of mac

111 cond laser microdissection (fs-LM), a single-cell isolation method that dissects specific cells direc

112 Conventional single-cell isolation methods often encounter operational compl

113 s as required by most of the existing single-cell isolation methods such as flow cytometry.

114 humans has been challenging due to a lack of cell isolation methods, and little is known about human

115 e ease of DNA sequencing without specialized cell isolation methods.

116 (1) Islet-cell isolation: miniature swine underwent either partial

117 OFPMS guarantees exact single cell isolation one by one just using a thermo-decomposab

118 onsequence of tissue injury sustained during cell isolation or organ retrieval and ischemia reperfusi

119 lored for T cell identification, traceless T cell isolation, or TCR characterization, respectively.

120 man whole blood (<3 microL) in an integrated cell isolation--PCR microchip containing a series of 3.5

121 We have now developed a simple cell isolation procedure to obtain and culture viable bi

122 ation systems demonstrated that the microcup cell isolation procedure yielded higher purity, yield, a

123 As only one mouse is needed for each cell isolation procedure, this protocol will be particul

124 cific expression clusters indicated that all cell isolation procedures also co-purify other unrelated

125 d induction of stress-inducible genes during cell isolation procedures.

126 loss of intracellular tacrolimus during the cell isolation process and is required to ensure adequat

127 Leukapheresis followed by a two-step cell isolation process yielded a CD34+ Thy-1+ cell popul

128 A refined cell isolation protocol and an improved flow cytometry a

129 Our cell isolation protocol and high-dimensional analysis al

130 The embryo cell isolation protocol can be completed in 5-6 h.

131 Our cell isolation protocol employed human fetal calvaria ti

132 D34 and CD45, we have developed a rapid oval cell isolation protocol with high yields of greater than

133 specific expression, investigators developed cell isolation, purification, and sorting protocols, yet

134 in vitro drug testing, cryopreservation, and cell isolation/purification.

135 itations, especially the lack of suture stem cell isolation, reconstruction of large craniofacial bon

136 pture, efficient cellular manipulation, rare-cell isolation, selective analytical separation of biolo

137 cive to these types of complex models due to cell isolation stress, cell loss, and high cost.

138 However, current methods for single cell isolation struggle to phenotypically differentiate

139 an donor bone marrow using an immunomagnetic cell isolation system.

140 d product compositions require high-purity T-cell isolation systems that, unlike immunomagnetic posit

141 The proposed target cell isolation technique can provide a practical means t

142 In conclusion, regardless of cell isolation technique, differential DNA methylation w

143 lls from 36 of 37 human hearts using primary cell isolation techniques and magnetic cell sorting tech

144 Existing cell isolation techniques cannot isolate neurons with sp

145 After cell isolation, the number of surface-sarcolemmal caveol

146 ysis and can be completed within a week from cell isolation through to quantification.

147 The entire process, from primary cell isolation to establishment of an immortal cell line

148 The protocol takes 12-15 h from single-cell isolation to library preparation and 3-7 d of data

149 The overall procedure from T-cell isolation to RV transduction takes 2 d, and enrichm

150 avoiding transcriptional changes induced by cell isolation trauma, as well as the identification of

151 Cell isolation via antibody-targeted magnetic beads is a

152 e behind the SCP-LVC-MS technology is single cell isolation via small droplet piezoelectric ejection

153 ive technique allows for in vivo labeling of cells, isolation via fluorescence-activated cell sorting

154 ts showed that the successful rate of single-cell isolation was about 90%.

155 s using uterine artery Doppler RI before dNK cell isolation, we have identified that impaired dNK-tro

156 ol (despite the long and invasive process of cell isolation) when metabolic rate at the physiological