ライフサイエンスコーパス: cell plate

1 ontrols (185 +/- 32 v 40 +/- 3 per 2 x 10(5) cell plated).

2 ant numbers of colonies (11-50 colonies/1000 cells plated).

3 nd membrane and callose are deposited at the cell plate.

4 targeting to the outward-growing rim of the cell plate.

5 d formed dense tubule-like structures in the cell plate.

6 icles to the CPAM and thereby to the growing cell plate.

7 the cytoplasm to the expanding and maturing cell plate.

8 , which is required for the formation of the cell plate.

9 for the synthesis of callose at the forming cell plate.

10 which later become integrated into the main cell plate.

11 of vesicle-tubule-vesicle structures at the cell plate.

12 ly localized to microtubules near the future cell plate.

13 e-vesicle structures observed on the forming cell plate.

14 ules are located near the site of the future cell plate.

15 e formation, and often results in an oblique cell plate.

16 redistributed to the growing margins of the cell plate.

17 d across the whole width of the newly formed cell plate.

18 on the phragmoplast microtubules and on the cell plate.

19 tein is associated with the formation of the cell plate.

20 abnormal callose accumulations formed at the cell plate.

21 sis, when the phragmoplast forms the nascent cell plate.

22 e ER abnormally buckled along the developing cell plate.

23 at directs vesicles secretion to the nascent cell plate.

24 the site where they coalesce to form the new cell plate.

25 sin form positively charged scaffolds in the cell plate.

26 molecules involved in the maturation of the cell plate.

27 ein) fusion protein was localized to growing cell plates.

28 cipates in the construction of newly forming cell plates.

29 ion and sorted by flow cytometry into single cell plates.

30 imals averaged 8.23 +/- 3.3601 per 3 million cells plated.

31 +/- 2.3523 osteogenic colonies per 3 million cells plated.

32 plus ends to deliver materials bound for the cell plate [2] [3].

33 esion molecule antibodies, and of 144 single cells plated, 39 clones were expanded, propagated, and s

34 The formation of the cell plate, a unique structure in dividing plant cells,

35 SAC9): in the Arabidopsis mutant sac9-3, the cell plate abnormally attaches at two equidistant positi

36 t most of its somata are confined to a dense cell plate adjacent to the fourth ventricle.

37 of defects, including branched or irregular cell plates, altered Golgi morphology and ectopic callos

38 re laterally displaced, and that the growing cell plate anchors on one side of the cell at an early s

39 Aurora2) associate with the spindle and the cell plate and are implicated in controlling formative d

40 mutant, reduced transport of vesicles to the cell plate and formed dense tubule-like structures in th

41 The localisation of NPK1 to the cell plate and its mitosis-specific activation suggest t

42 Callose is synthesized on the forming cell plate and several other locations in the plant.

43 his protein is involved in maturation of the cell plate and the re-establishment of cytoplasmic actin

44 Immunolabeling experiments show that the cell plates and cell walls of the endosperm differ from

45 rotein located in the nascent cross wall or "cell plate" and also in mature cell walls.

46 to appear first in the center of the forming cell plate, and as the cell plate grew outward, it redis

47 ls enhanced callose synthesis on the forming cell plate, and that these cell lines exhibited higher l

48 ective microspores did not form a continuous cell plate, and two identical nuclei were produced with

49 otein responses, discussing surface coating, cell plating, and fluorescence imaging in detail.

50 ass of nanolattices, constructed from closed-cell plate-architectures.

51 plant cytokinesis, including assembly of the cell plate, are not fully understood.

52 required to initiate, assemble and shape the cell plate as it grows toward the mother cell cortex is

53 Using Caco-2 colonic epithelial cells plated as a polarized monolayer in transwells, we

54 CD34(+)/c-kit(+)/CD13(+) cells plated as single cells in the presence of various

55 Clonogenic (single-cell plating) assays were used to define and quantify su

56 nsisting of a filamentous ribosome-excluding cell plate assembly matrix (CPAM) and Golgi-derived vesi

57 ween vesicles and regulatory proteins at the cell plate assembly matrix during polysaccharide deposit

58 At their equatorial planes, cell plate assembly sites are formed, consisting of a fi

59 l1A-2 adl1E-1 double mutants show defects in cell plate assembly, cell wall formation, and plasma mem

60 s matrix contains the molecules that mediate cell plate assembly.

61 ning between daughter cells as well as rapid cell plate assembly.

62 aracterization of patellin1 (PATL1), a novel cell-plate-associated protein that is related in sequenc

63 CalS1 interacts with two cell plate--associated proteins, phragmoplastin and a no

64 o constrict the ER, was not recruited to the cell plate at cytokinesis.

65 ent in the PD proteome, are recruited to the cell plate at late telophase, when primary PD are formed

66 We show that NCS cells plated at clonal density give rise to multiple neu

67 higher functional HIF-1alpha expression than cells plated at high density independent of O2 tension.

68 to ICE family proteases or by aggregation of cells plated at high density.

69 ed at lower densities release more PGE2 than cells plated at higher densities.

70 the keratocyte markers in 7-day cultures in cells plated at low (5,000 cells/cm2) and high (20,000 c

71 Inhibition of FAK or Src in MCF-10A cells plated at low cell density prevented the activatio

72 ous manner, as indicated by its detection in cells plated at low density and in cultures in which dep

73 Human prostate cancer cells plated at low density manifested higher functional

74 Cells plated at lower densities release more PGE2 than c

75 hragmoplast microtubules (AtMAP65-4) and the cell plate (AtKNOLLE) were indistinguishable from those

76 ed eukaryotic proteins to distinct stages of cell plate biogenesis and emphasize the coupling of cell

77 , are essential for polar cell expansion and cell plate biogenesis.

78 t to the involvement of phosphoinositides in cell-plate biogenesis.

79 nts, including surface functionalization and cell plating, can be completed in 10 h.

80 at is required for callose deposition at the cell plate, cell wall and plasmodesmata.

81 n the recruitment or removal of a variety of cell plate components; thus, they did not demonstrate a

82 e was enhanced by low doses of inhibitors of cell plate consolidation and vesicle secretion.

83 lagenase stimulatory activity and epithelial cell plating density was demonstrated.

84 se B by these cells is similarly affected by cell plating density.

85 In contrast to this picture, we observe that cell-plate development in Arabidopsis shoot cells is hig

86 ntly from primary cultures of dissociated TG cells plated directly after removal from the mouse (7 an

87 reticulum (ER) during interphase and at the cell plate during cell division.

88 the size quantification of vesicles near the cell plate during cell division.

89 essential for the correct positioning of the cell plate during cytokinesis in cells of the developing

90 ily of proteins, becomes associated with the cell plate during cytokinesis in plants.

91 ic protein is functional and targeted to the cell plate during cytokinesis in transgenic cells.

92 In higher plants, the formation of the cell plate during cytokinesis requires coordinated micro

93 h in turn direct the vesicles to the forming cell plate during cytokinesis.

94 rent patterns of localization at the forming cell plate during cytokinesis.

95 es in interphase cells and with the immature cell plate during cytokinesis.

96 T1 fusion protein was located on the forming cell plate during cytokinesis.

97 s, and lipids are trafficked to and from the cell plate during cytokinesis.

98 to the apex of tip-growing cells and to the cell plate during cytokinesis.

99 Construction of the cell plate during plant cell division requires the preci

100 rafficking is central to construction of the cell plate during plant cytokinesis.

101 the TGN, the protein is targeted to growing cell plates during cell division.

102 that orchestrate the growth and guidance of cell plates during cytokinesis.

103 irs and pollen tubes or de novo formation of cell plates during plant cytokinesis, newly synthesized

104 plane that minimizes the surface area of the cell plate (Errera's rule) while creating daughter cells

105 d distinguished three distinct phases during cell plate expansion in tobacco (Nicotiana tabacum) 'Bri

106 membrane-trafficking events associated with cell-plate expansion or maturation and point to the invo

107 on tomography, we mapped the transition from cell plate fenestrae to plasmodesmata.

108 like other gsl8 mutants, in which defects in cell plate formation are seedling lethal, cytokinetic de

109 een isolated and shown to be associated with cell plate formation in soybean by using immunocytochemi

110 ragmoplastin, it appears that the process of cell plate formation is completed in two phases.

111 pan1 mutants exhibited no defects in cell plate formation or in the recruitment or removal of

112 ate biogenesis and emphasize the coupling of cell plate formation with phragmoplast expansion.

113 Cell plate formation within the CPAM appears to be initi

114 ant negative, slowing down the completion of cell plate formation, and often results in an oblique ce

115 accurate phragmoplast fusion, and subsequent cell plate formation, at the preprophase band site.

116 nizes PME5 messenger RNA (mRNA) release with cell plate formation, enabling precise demethylesterific

117 cles, which accumulate at the early stage of cell plate formation, were not affected by ES7, KNOLLE w

118 trafficking and fusion machinery involved in cell plate formation.

119 tes in an early membrane fusion event during cell plate formation.

120 s to monitor the dynamics of early events in cell plate formation.

121 wn about the forces that model the ER during cell plate formation.

122 esis in the microspore that is essential for cell plate formation.

123 provide a mechanically robust mechanism for cell-plate formation in large cells and suggests a simpl

124 Here we show that membranous material for cell-plate formation initially accumulates along regions

125 k for how the cytoskeleton spatially defines cell-plate formation is lacking.

126 smata (PD) arise at cytokinesis when the new cell plate forms.

127 novel kind of cell plate, the syncytial-type cell plate, from Golgi-derived vesicles approximately 63

128 callose persists in the cell walls after the cell plates fuse with the parental plasma membrane.

129 During cytokinesis, the expanding cell plate fuses with the plasma membrane at the cortica

130 heral cell plate growth zone, which leads to cell plate fusion with the cell wall.

131 center of the forming cell plate, and as the cell plate grew outward, it redistributed to the growing

132 maximum during the late PFS stage, when most cell plate growth is completed.

133 eates the centrifugally expanding peripheral cell plate growth zone, which leads to cell plate fusion

134 tubular networks, giving rise to new foci of cell plate growth, which later become integrated into th

135 s suggested to be responsible for regulating cell plate growth.

136 Recent studies of membrane fusion at the cell plate have revealed the contribution of functionall

137 ence microscopy, NSPN11 was localized to the cell plate in dividing cells.

138 tin has been shown to be associated with the cell plate in dividing cells.

139 punctate subcellular structures and with the cell plate in dividing cells.

140 fluorescence microscopy localized PDL to the cell plate in dividing soybean root tip cells.

141 t cell is accomplished by the formation of a cell plate in the center of the phragmoplast.

142 PAN1, but not PAN2, is localized to cell plates in all classes of dividing cells examined.

143 e-dimensional architecture of syncytial-type cell plates in the endosperm of Arabidopsis has been ana

144 Single cell plating in serum-free medium allows direct assessme

145 observed spread of infection to nonneuronal cells plated in a different compartment.

146 aggregated fail to differentiate, but mutant cells plated in a wild-type background are able to do so

147 In contrast, the substratum of subconfluent cells plated in noncoated dishes lacked vitronectin but

148 ng term bone marrow cultures, or bone marrow cells plated in semisolid medium.

149 For these determinations, cells plated in serum-free medium were treated either wi

150 e phosphorylation of VEGFR-2 was observed in cells plated in sparse culture conditions (60-65% conflu

151 servation of elevated levels of p27(KIP1) in cells plated in the presence of fibrillar collagen has l

152 Cells plated in the presence of fibrillar collagen were

153 (KOR) gene causes the formation of aberrant cell plates, incomplete cell walls, and multinucleated c

154 Cell plates induced to insert outside the predicted divi

155 Whether cells plated into 3D cultures give rise to a singular ph

156 t cytoskeleton has depolymerized and the new cell plate is beginning to mature.

157 The cell plate is formed by the fusion of Golgi apparatus-de

158 aped, membrane-enclosed precursor termed the cell plate is formed that radially expands toward the pa

159 distribute around the phragmoplast where the cell plate is formed.

160 that polarized targeting of KOR1 to forming cell plates is essential for cytokinesis.

161 d with exocytic vesicles that are depositing cell plate material during cytokinesis in the plant cell

162 al role of callose during the late stages of cell plate maturation and establish the temporal relatio

163 urprising and fundamental role for the ER in cell plate maturation.

164 tions of secretory and endosomal vesicles in cell plate maturation.

165 t recycling at the PM, vacuolar sorting, and cell-plate maturation.

166 daughter cells across fenestrae, and as the cell plate matures, fenestrae contract, causing the plas

167 dopsis RanGAP1 is associated with the NE and cell plate, mediated by an N-terminal, plant-specific WP

168 Without shortening, the wider cell-plate membrane depositions evolved into cell walls

169 A ribosome-excluding matrix encompasses the cell plate membranes from the fusion of the first vesicl

170 ctopic expression, ARL2 is also found at the cell plate of dividing cells during cytokinesis, an area

171 ell surface and accumulates at the expanding cell plate of dividing cells.

172 opy indicates that ADL1C is localized to the cell plate of dividing somatic cells and to the tip of e

173 A cell plated on a two-dimensional substrate forms adhesio

174 f a Boyden chamber and the rest of the liver cells plated on a cell culture insert, and fish oil or f

175 Whether and how cells plated on a two-dimensional matrix or embedded in

176 ubcB-null cells plated on agar form mounds with normal kinetics; h

177 ACh withdrawal was significantly smaller in cells plated on an alpha beta 1-integrin antibody (10 +/

178 -induced stimulation of ICa,L was greater in cells plated on an alphabeta1-integrin antibody than on

179 Cleavage of RhoA was detectable in BAE cells plated on an integrin substrate; it did not occur

180 stiffness at the cellular level for MC3T3-E1 cells plated on biomaterial substrates of varying modulu

181 Only RPE cells plated on cleaned or cleaned and ECM-coated ICL de

182 nin-5, and had no effect on reaggregation of cells plated on collagen I (alpha2beta1 integrin ligand)

183 However, endothelial cells plated on collagen I were resistant to TGF-beta1-i

184 Human umbilical vein endothelial cells plated on collagen I-coated plates and cultured in

185 lphavbeta5 inhibited degradation (70-90%) by cells plated on collagen or laminin.

186 A is essential for the active stiffening of cells plated on collagen-coated substrates.

187 ls plated on fibronectin but 0.35 ng/min for cells plated on collagen.

188 onized with IgG or complement, compared with cells plated on control protein.

189 f CLANs and quantitate CLAN formation in HTM cells plated on coverslips coated with various extracell

190 Analyses of invadopodia function from cells plated on cross-linked gelatin and collagen gels u

191 n, using live-cell imaging of invadopodia in cells plated on cross-linked gelatin, was consistent wit

192 trap micrometer-sized beads internalized in cells plated on crossbow-shaped adhesive micropatterns a

193 typic modulation of VSMCs, whereby senescent cells plated on ECM synthesized from cells depleted of S

194 Moreover, cells plated on extracellular matrix-coated coverslips s

195 Activation of PAK is higher in cells plated on fibronectin (FN) compared to basement me

196 [125I]vitronectin, which was 3.0 ng/min for cells plated on fibronectin but 0.35 ng/min for cells pl

197 f focal adhesions and actin stress fibers by cells plated on fibronectin depends on adhesion-mediated

198 Whereas endothelial cells plated on fibronectin or fibrinogen activate NF-ka

199 eta 1-containing sites of focal adhesions on cells plated on fibronectin or the III-9,10 modules of f

200 Translocation of YopH into cells plated on fibronectin resulted in rapid and select

201 Piezo1 expressing HEK cells plated on fibronectin stripes elongated, while a k

202 In cells plated on fibronectin, FAK could indeed autophosph

203 combined effect 70%) of [125I]vitronectin by cells plated on fibronectin, only mAb anti-alphavbeta5 i

204 ignificantly retarded the spreading of REF52 cells plated on fibronectin.

205 phosphorylation in alpha(5)-deficient muscle cells plated on fibronectin.

206 reased IGFBP-5 gene expression 3-fold in the cells plated on fibronectin.

207 ere continuously elevated in PRL-1 knockdown cells plated on fibronectin.

208 uced here showed that mu1B-knocked down MDCK cells plated on filters at confluency and cultured for 4

209 ntegrins into focal contacts in alpha 5-null cells plated on FN, indicating that alpha V integrins ca

210 phosphorylation of Tyr(397) to match that of cells plated on FN.

211 In contrast, FN-null cells plated on FNIII(8-11) contiguous with FN-GFBD surv

212 Cells plated on glucose-modified collagen IV showed redu

213 ces of spreading and differentiation of stem cells plated on hydrogel and silicone gel substrates on

214 fibers indistinguishable from those seen in cells plated on intact fibronectin.

215 , elicited a greater stimulation of ICa,L in cells plated on laminin (+79 +/- 16 %; n = 17) than on g

216 ith 50 microM cAMP was not different between cells plated on laminin or glass.

217 ed cAMP content was significantly smaller in cells plated on laminin than on glass.

218 olin (1 microm) was significantly smaller in cells plated on laminin than on glass.

219 SCLC cells plated on laminin were not only resistant to serum

220 o-localize in retraction fibers in carcinoma cells plated on laminin, a finding suggesting a function

221 Cells plated on laminin-5 for 16 d express increased lev

222 Mouse mesangial cells plated on MGO-modified collagen IV showed decrease

223 defines the rear instead of the front, using cells plated on micropatterned adhesive strips to facili

224 tion were impaired in migrating cells and in cells plated on micropatterned substrates, and cell migr

225 Cells plated on monoclonal antibody 281.2 initially exte

226 n was cell substrate-dependent since type II cells plated on plastic dishes did not show this effect.

227 Cells plated on PN form fewer stress fibers and are more

228 ial restoration of cell cycle progression in cells plated on poly-L-lysine, providing further support

229 n an integrin substrate; it did not occur in cells plated on poly-l-lysine.

230 ression of FAK in serum-starved glioblastoma cells plated on recombinant (rec)-osteopontin resulted i

231 FAK further enhances invadopodia activity in cells plated on rigid polyacrylamide substrates.

232 Nonpigmented cells plated on soft collagen gels retained a rounded sh

233 In experiments on human mesenchymal stem cells plated on soft, medium rigidity, and hard silicone

234 The level of these microRNAs increased in cells plated on stiff versus soft substrates, consistent

235 activates specific integrins in endothelial cells plated on substrates containing the cognate extrac

236 ed 70-kDa fragments to cycloheximide-treated cells plated on the 160-kDa substrate, suggesting that a

237 emotaxis induced by PDGF-BB were enhanced in cells plated on the alphavbeta3 ligand vitronectin compa

238 alphavbeta3 ligand vitronectin compared with cells plated on the beta1 integrin ligand collagen.

239 f focal adhesions and actin stress fibers in cells plated on the cell-binding domain of fibronectin c

240 protein levels were very low in endothelial cells plated on the non-integrin cell attachment factor,

241 beta 1/TM4 complexes toward the periphery of cells plated on various extracellular matrix substrates

242 The spreading of a cell plated onto a substrate is one of the simplest para

243 ised of hepatocytes and 3T3-J2 mouse stromal cells plated onto a patterned standard 96-well or 24-wel

244 RPE cells plated onto BL repopulated the explant surface wit

245 Cells plated onto collagen or laminin, which engage diff

246 xplant surface within 14 +/- 3 days, whereas cells plated onto the ICL and EL eventually died and nev

247 In contrast, cells plated onto the laminin-5-rich matrices of pp126 e

248 Human corneal endothelial cells plated onto the matrices elaborated by bovine corn

249 Further analysis of the cells plated onto vitronectin indicated that PDGF stimul

250 he disturbance of the directional control of cell plate orientation.

251 increased in proportion to the number of MMQ cells plated out.

252 e, based on their prolonged staining for the cell-plate polymer callose.

253 nation of the number of living cells through cell plating procedure after cultivations is known as ti

254 Utilizing a cell plating protocol, we successfully isolate FAPs-rich

255 ilar polarity developed in phragmoplasts and cell plates, raising the possibility that polarized divi

256 The CPAM, which is found only around growing cell plate regions, is suggested to be responsible for r

257 fferentiated root cells and targeting to the cell plate remain intact.

258 Growth of the cell plate requires continuous fusion of vesicles, and p

259 ts of the dynamics of vesicle markers on the cell plate revealed an overall reduction of the delivery

260 ates the deposition of callose at developing cell plates, root hairs, and plasmodesmata.

261 An Arabidopsis gene encoding a putative cell plate-specific callose synthase catalytic subunit (

262 The interplay between cell plate-specific post-Golgi vesicle traffic and callo

263 most well-characterized component, KNOLLE, a cell plate-specific soluble N-ethylmaleimide-sensitive f

264 That CalS1 is a cell plate--specific enzyme is demonstrated by the obser

265 e mechanism ensuring the maturation of those cell plates successfully contacting the "programmed" cor

266 abolish targeting to the nuclear rim and the cell plate, suggesting that the same mechanism is involv

267 mitotic cells and their localisation to the cell plate suggests a role in cytokinesis.

268 h the phragmoplast initials and with the TVN cell plate that is formed within the solid phragmoplast.

269 fusion protein was localized at the growing cell plate, that expression of CalS1 in transgenic tobac

270 e mini-phragmoplasts produce a novel kind of cell plate, the syncytial-type cell plate, from Golgi-de

271 rane may play important roles in guiding the cell plate throughout much of its development.

272 hind by the PPB and is proposed to guide the cell plate to the cortical division site is unknown.

273 oplast expands centrifugally and directs the cell plate to the preselected division site at the plasm

274 polarization reorientation solitons from the cell plates to the cell center upon field reversal.

275 t by forming a new membrane compartment, the cell plate, via a rerouting of the secretory pathway tow

276 vo and colocalize with RanGAP1 at the NE and cell plate were identified.

277 LBs are mobile at the edge of the developing cell plate, where new wall materials are being delivered

278 he plant-specific cytokinetic organelle, the cell plate, which develops across the division plane and

279 ssemble a new intracellular compartment, the cell plate, which grows centrifugally by vesicle fusion

280 sis was abolished without the formation of a cell plate, which led to failures in the birth of the ge

281 concomitant with cell expansion, but not to cell plates, which depend on actin and CSLD for structur

282 ndocytic membrane remodeling in the maturing cell plate while the plate is stabilized by callose.

283 simple PDs are inserted into the developing cell plate, while during wall extension, more complex (b

284 r at the end of interphase and predict where cell plates will fuse with parental walls during divisio