ライフサイエンスコーパス: cell-based

1 Human stem cell-based alveolospheres, thus, provide novel insights

2 ks (cDNNs), ResNet50 or ResNet18, and single cell-based analysis, we found that Disc1-KD decreased th

3 In this study, we used a combination of cell-based and biochemical assays to show that the mode

4 Herein, we review current cell-based and biomolecular (primarily small-molecule an

5 Using cell-based and in vitro assays, we demonstrate that the

6 shed, while another thirteen pairs have both cell-based and in vitro evidence but lack independent re

7 s negative regulators of translation in both cell-based and in vitro translation assays.

8 Together, our genetic, cell-based, and in vivo modeling data delineate an autos

9 Biophysical, cell-based, and murine xenograft experiments demonstrate

10 enerated growing enthusiasm as an innovative cell-based approach in solid organ transplantation (SOT)

11 erneuron transplantation could offer a novel cell-based approach to autism treatment while challengin

12 Human CD83 CAR T cells are a promising cell-based approach to preventing 2 critical complicatio

13 To establish a stem cell-based approach to restoring TM function and normali

14 irections to tackle these voids using single-cell-based approaches and unique tissue sampling models.

15 Conventional alphabeta CAR-T cell-based approaches have revolutionized the field of c

16 henotype, (2) MOG-IgG seropositivity by live cell-based assay and (3) MRI lesion(s) of brainstem, cer

17 zymes, we developed a robust and automatable cell-based assay based on fluorophore- and fluorescence-

18 Here, we introduce a cell-based assay for protein-protein interaction analysi

19 ania NMT, across 68 compounds in enzyme- and cell-based assay formats.

20 tested BCRs were autoreactive, although the cell-based assay sensitively detects feeble Ag recogniti

21 A cell-based assay shows that nonhomologous end joining (N

22 We validated that a cell-based assay that measures TNF-alpha production by C

23 To address this challenge, we developed a cell-based assay that utilizes a premalignant phenotype

24 We describe a cell-based assay to overcome this challenge, based on GP

25 We developed a cell-based assay to screen the ability of CCG258747 and

26 ty to trastuzumab, has similar activity in a cell-based assay, and can arrest tumor growth in a mouse

27 In a cell-based assay, TAF was 35- and 24-fold and TDF was 10

28 This sequential cell-based assay, which examines the monocyte and eBm5 c

29 tested for NMDAR IgG antibodies using a live cell-based assay.

30 S autoimmune disorders, using fixed and live cell-based assays (CBAs).

31 onclude that acute transcriptomic changes in cell-based assays combined with drug substructures are p

32 r matrix while surface plasmon resonance and cell-based assays confirmed that ACE-083 binds and poten

33 Cell-based assays demonstrate nanomolar inhibition (EC(5

34 Miniaturization of cell-based assays enables the analysis of secreted compo

35 d underscores the utility of high-resolution cell-based assays for assessment of compound efficacy.

36 In recent years, cell-based assays have gained more and more popularity s

37 dentify a candidate gene, ALG9, and in vitro cell-based assays of PC1 protein maturation to functiona

38 We describe here two cell-based assays of tau inclusion formation that we emp

39 iomolecular labels and tags while label-free cell-based assays only offer holistic information about

40 Cell-based assays showed that OsPHT2;1 localized to the

41 CD2 monoubiquitination in biochemical and/or cell-based assays tended to show earlier onset of hemato

42 vide an overview of established and emerging cell-based assays that exploit fluorescence for studies

43 Here, we demonstrate in vitro and in cell-based assays that the viral RNA polymerase, NS5, in

44 nity to assimilate biochemical findings with cell-based assays to uncover new insights into this dece

45 opment as well as the establishment of human cell-based assays to validate therapeutic strategies ex

46 lar to upper nanomolar inhibitory potency in cell-based assays, are selective against other serine pr

47 displayed substantially enhanced potency in cell-based assays, as well as in murine tumor xenograft

48 g a combination of in vitro biochemistry and cell-based assays, including chemically induced dimeriza

49 In live cell-based assays, LGI1 epitope recognition was examined

50 cted mutagenesis, inhibition experiments and cell-based assays, the structure also provides an insigh

51 rticipants, disease modeling in rodents, and cell-based assays, we examined whether S1P signaling may

52 Using engineered mouse models and cell-based assays, we provide evidence that the Tug1 loc

53 icroscopy, structure-guided mutagenesis, and cell-based assays, we reveal the binding interactions be

54 rum aquaporin-4 antibodies are detected with cell-based assays.

55 ent in vitro, do not always show efficacy in cell-based assays.

56 so exhibited promising antiviral activity in cell-based assays.

57 ed kinases to drug treatment in patients and cell-based assays.

58 zoRosi, which were confirmed to be active in cell-based assays.

59 ar inhibition in DENV RNA polymerization and cell-based assays.

60 ted their MT binding properties in mammalian cell-based assays.

61 inhibitors of the enzyme in biochemical and cell-based assays.

62 maging, proteomic, genetic, metabolomic, and cell-based assays.

63 e high potency of RDV against RNA viruses in cell-based assays.

64 o the observed [Formula: see text] values in cell-based assays.

65 igh-throughput screening using enzymatic and cell-based assays.

66 Using a cell-based bimolecular fluorescence complementation assa

67 existing equilibrium partitioning model for cell-based bioassays extended by an additional lipid pha

68 Here, we report the development of a stable cell-based bioluminescence assay of the transcription fa

69 of of concept, a rapid assay consisting of a cell-based biosensor (CBB) panel of pure bacterial strai

70 This allows cell based biosensors to respond more rapidly and sensit

71 developments have been made in the design of cell-based biosensors in the last about five years.

72 iated delivery, including receptor-targeted, cell-based, blood-brain-barrier disrupting and MRI-guide

73 dariz et al (2020) report a murine lung stem cell-based bronchioalveolar organoid system and provide

74 t vs. a library of 645,000 compounds using a cell-based calcium detection system.

75 aluated using classic in vitro AC assays and cell-based cAMP accumulation assays in COS-7 cells.

76 EC(50) of 59 nM in the GPR88 overexpressing cell-based cAMP assay.

77 d to discuss the progress and problems of NK cell-based cancer immunotherapies.

78 at interest clinically for the generation of cell-based cancer immunotherapies.

79 assemblies that combine natural killer (NK) cell-based cancer immunotherapy with radiotherapy and ch

80 cancer cells is important for the success of cell-based cancer immunotherapy.

81 Subsequently, the field of NK cell-based cancer therapy has grown exponentially and cu

82 The outcome of the cell-based cannabinoid reporter system, which monitored

83 uch derivatives could be adapted into either cell-based (CAR-T) or protein-based (bispecific T-cell e

84 ll wall particles (YCWPs) were used as model cell-based carriers with distinct intracellular composit

85 oaccessibility of encapsulated compound from cell-based carriers.

86 ring simulated gastrointestinal digestion of cell-based carriers.

87 In human immune cell-based chemotaxis assays, we find that CXCL12-stimul

88 osis model, as well as novel 2D and 3D human cell-based co-culture of human hepatocytes, KCs (Kupffer

89 Furthermore, we employ cell-based complementation studies in mouse embryonic fi

90 ransferable designs are then created using a cell-based construction toolkit to realize living system

91 contrast, the isolation of SARS-CoV-2 using cell-based culture likely indicates infectivity, but the

92 ves debris-contaminated droplets from single-cell-based data fast and effectively, leading to cleaner

93 Consistent with cell-based data, adoptive transfer experiments demonstra

94 Herein, we outline the development of a cell-based delivery system using macrophages as a delive

95 also discuss the mechanisms regulating stem cell-based dental tissue homeostasis, regeneration and r

96 Due to its unbiased nature, this new cell-based detection method presents itself as an invalu

97 enome stability is essential for engineering cell-based devices and reporter systems.

98 major virulence genes and developed a single-cell-based dPCR approach for rapid (within 1 day) and ac

99 Cell-based drug delivery systems have generated an incre

100 Here, we used a cell-based ELISA (CBE) assay to evaluate each component

101 ck independent replication and/or sufficient cell-based evidence.

102 t predictions, based on both preclinical and cell-based experimental findings.

103 Here, we discuss in vitro and cell-based experimental strategies used to identify KDAC

104 NA-directed DNA recognition, biochemical and cell-based experiments establish scoutRNA as an essentia

105 nome-barcoded sensors, reporter systems, and cell-based factories.

106 Altogether, our cell-based findings together with biochemical studies su

107 ted for AQP4-IgG and MOG-IgG by using a live-cell-based flow cytometry assay.

108 nstrated attenuated homodimer formation in a cell-based fluorescence complementation assay.

109 No significant offsite toxicity of the cell-based formulations in mouse brain and peripheral or

110 nalysis combined with RNA-binding assays and cell-based frameshifting reporter assays reveal a number

111 iants was analyzed in a mouse embryonic stem cell-based functional assay.

112 fied by this method have purity suitable for cell-based functional assays and in vivo studies.

113 entify new regulators of let-7, we devised a cell-based functional screen of RNA-binding proteins usi

114 involves labor-intensive embryonic stem (ES) cell-based gene targeting and tedious micromanipulations

115 ow to all H3N2 viruses tested, including the cell-based H3N2 strain.

116 on assays using an egg-adapted H3N2 virus, a cell-based H3N2 virus, wild-type 3c2.A and 3c2.A2 H3N2 v

117 Here, we have developed a novel co-culture cell-based high throughput assay system to identify comp

118 ompounds can be measured by many in vitro or cell-based high throughput assays that record various en

119 We report here a cell-based high-throughput screen of a library containin

120 ion of CCR5-mutant MCMs to assess novel stem cell-based HIV therapeutics.

121 le for renewed efforts to develop a CD8(+) T cell-based HIV vaccine in conjunction with B cell vaccin

122 for rational immunogen design, including a T cell-based HIV vaccine.

123 Sb9-deficient mice exhibited protective T cell-based host immunity to tumors in association with a

124 carriers, on cellular surfaces to generate a cell-based hybrid system referred to as "Cellnex" is est

125 ting that it could enable cross-protective T-cell-based immunity.

126 ation of HPK1 improves the efficacy of CAR-T cell-based immunotherapies in diverse preclinical mouse

127 novel strategy to increase the efficacy of T-cell-based immunotherapies in nonresponding individuals.

128 Failed T cell-based immunotherapies in the presence of genomic al

129 The recent success of engineered cell-based immunotherapies now provides a mechanism to i

130 The full potential of T cell-based immunotherapies remains limited by a variety

131 Checkpoint inhibitors, oncolytic viruses, cell-based immunotherapies, cytokines, and adjuvants hav

132 notherapy and has general implications for T cell-based immunotherapies.

133 s are important for the improvement of CAR-T cell-based immunotherapy for human cancers.

134 ective CD8(+) T cells and pave the way for T-cell-based immunotherapy to combat recurrent ocular herp

135 transiently inhibiting LDH during adoptive T cell-based immunotherapy, with an unanticipated cooperat

136 presentations pathways may be overcome by NK cell-based immunotherapy.

137 as also confirmed in Proteus mirabilis whole-cell-based inhibition assays.

138 Ts)/thrombin axis, using COVID-19 specimens, cell-based inhibition studies, and NET/human aortic endo

139 le of cell chirality in development, nonstem cell-based injury repair, cancer-promoting processes, an

140 eases at the single-cell level will usher in cell-based interceptive medicine in Europe over the next

141 atomistic simulations totaling 32.7 mus and cell-based intramolecular fluorescence resonance energy

142 us CD34(+) hematopoietic stem and progenitor cell-based lentiviral gene therapy following myeloablati

143 We established a cell-based LIPG enzymatic assay system to measure the in

144 In this study, we developed a cell-based MALDI MS workflow for investigation of OATP2B

145 nd gastric cancer, as well as multiple blood cell-based malignant tumors, such as lymphoma.

146 s at play in atherosclerosis will facilitate cell-based mapping of novel interventional targets with

147 e benefits and challenges of plant-based and cell-based meat alternatives with regard to production e

148 ough cellular agriculture methodology (i.e., cell-based meat).

149 Use of cell-based medicinal products (CBMPs) represents a state

150 that can promote heterochromatin, we used a cell-based method and screened NCI drug libraries consis

151 quatic bacteria detection methods, including cell-based methods, nucleic acid amplification detection

152 ence of a poly-glutamine aggregation protein cell-based model of Huntington's disease.

153 PR interference (CRISPRi) screens in a human cell-based model of propagation of tau aggregation monit

154 Notably, disrupting YIPF5 in beta cell-based models induced ER stress signaling and result

155 In neuronal cell-based models of mTOR hyperactivity, 7 corrected the

156 motivated the development of improved human cell-based models to bridge the gap between well-plate a

157 Using animal and cell-based models, we show that FABP4 is secreted from w

158 the mechanistic understanding of AD, single cell-based molecular alterations are largely unknown.

159 presents a high-throughput and versatile ES cell-based mouse modeling platform that can be combined

160 induce graded persistent activity - a single-cell based, multistable mnemonic firing mode experimenta

161 binant protein-based, n = 23), or Flucelvax (cell-based, n = 23) during the 2017-2018 season.

162 ombinant protein-based, n=23), or Flucelvax (cell-based, n=23) during the 2017-2018 season.

163 tic impact on RNAPII elongation rates in our cell-based nascent RNA-seq experiments upon Elongin A de

164 n in vivo readout for ototoxicity and kidney cell-based nephrotoxicity assay, we screened 1280 compou

165 These mutations decreased activity in cell-based NER assays, demonstrating the functional impo

166 put assays in human induced pluripotent stem cell-based neurodevelopmental models to probe genetic ri

167 handling that is able to distinguish between cells based not only on viability but cell type, develop

168 Using cell-based Notch signaling and ligand-binding assays, we

169 duals will help in the potential design of T-cell-based ocular herpes vaccines.

170 pose a concept of a microfabricated analysis cell based on an IR-transparent Si membrane with advanta

171 ely, the latter of which doubles that of the cell based on graphite anodes.

172 AEOT generates an artificial lineage of this cell based on the population characteristics of the othe

173 million molecules or 0.5-0.7 pg protein per cell; based on our in-house developed NPM-ALK ELISA; LOD

174 e CK2 with significant cytotoxicity on tumor cells based on a 2-aminothiazole scaffold were described

175 for Ca(2+) and CaNAbeta compartmentation in cells based on an isoform-specific mechanism for calcine

176 has allowed for immunophenotyping of single cells based on cell surface expression of specific prote

177 ermining the power density of microbial fuel cells based on dimensional analysis.

178 ovides a facile and robust way of separating cells based on elevated glycolytic activity; a biomarker

179 ut also good performance (for example, solar cells based on lead-tin-gradient structures with an aver

180 ), a Bayesian hierarchical method to cluster cells based on local methylation patterns, discovering p

181 as resulted in the highest performance solar cells based on mixtures of Cs, methylammonium, and forma

182 patterned the fast VCS into slow, nodal-like cells based on molecular and functional criteria.

183 Organic solar cells based on non-fullerene acceptors can show high cha

184 Solar cells based on organo-metal halide perovskites have gain

185 desaturase SCD1 may selectively kill cancer cells based on our discovery of spatial separation of fa

186 w strategy for the chemical analysis of live cells based on protein spherical nucleic acids (ProSNAs)

187 putational approach to classify thousands of cells based on published data within seconds while simul

188 In contrast, cells based on pure ceramic and pure polymer electrolyte

189 o-occurrence clustering algorithm to cluster cells based on the dropout pattern.

190 cytometric strategy to detect clonal Sezary cells based on the monotypic expression of one of two mu

191 boundaries and graph partitioning to segment cells based on the neural network predictions.

192 n be detected and separated from bystander T cells based on their cell-surface enzymatic fucosyl-biot

193 hly specific capture rates of bladder cancer cells based on their Epithelial Cell Adhesion Molecule e

194 IL-2C) to target IL-2 to distinct subsets of cells based on their expression of subunits of the IL-2R

195 inds application in the enrichment of single cells based on their micrographs for further downstream

196 ography, the sorting of large populations of cells based on their migratory characteristics, cannot b

197 Spatial manipulation of suspended cells based on their properties is an essential part of

198 millions of live migratory cancer and immune cells based on their spontaneous migration in two-dimens

199 We propose that indole spatially segregates cells based on their state of adaptation to repel invade

200 nt cycling method for the analysis of single cells based on unique linkers for tetrazine (Tz)/trans-c

201 ersus those derived from less invasive tumor cells, based on dielectrophoresis measurements.

202 regulation of Dicer expression in monocytic cells, based on proteolysis.

203 Characterizing these cells based only on surface markers using flow cytometry

204 The first kidney was perfused with red cell-based perfusate at 37 degrees C for 75 min, mean re

205 underwent a 1-hour period of NMP using a red cell-based perfusate.

206 osis inducing SP degradation, resulting in a cell-based product alternative to BM-MSC to potentially

207 s, expose patients to potentially risky stem cell-based products, and undermine efforts to develop ev

208 This multi-omics and cell-based profiling showed an immediate redirection of

209 Preclinical advances in cell-based, protein replacement and gene therapies are p

210 Here, using a cell-based proteomics technique called multiplexed kinas

211 ins (SRIGs) allow the standardisation of the cell-based rapid fluorescent-focus inhibition test (RFFI

212 such as the presence of PAINs motifs and the cell-based readout using compounds that are weakly poten

213 A new article combining biophysical and cell-based readouts of 15 mutants of the preQ(1)-II ribo

214 d gingiva can be used for the development of cell-based regenerative approaches in dentistry and medi

215 ale for Nrf2 as a therapeutic target in stem cell-based regenerative medicine.

216 at SCI effectively and that a combination of cell-based, rehabilitation and pharmaceutical interventi

217 sis, in autoimmune diseases, and during stem cell-based repair processes after stroke and myocardial

218 By gene mapping and with the help of a novel cell-based reporter assay system we characterized the fi

219 latory activity was analyzed further using a cell-based reporter assay.

220 ity of endogenous IL1RA was assessed using a cell-based reporter assay.

221 ound in human cancers using a combination of cell-based reporter assays, genome editing, flow cytomet

222 dition to enabling mechanistic studies, this cell-based reporter may enable identification of small m

223 Participants received a single dose of a cell-based, reverse-genetics, Good Manufacturing Practic

224 n vivo, we developed and implemented a novel cell-based screen using Actinomyces oris, a key colonize

225 From a pathway-targeted cell-based screen, we identified a non-nucleotide, small

226 Skeletal muscle progenitor cell-based screening identified three compounds that enh

227 d be a simple end point detection method for cell-based screening of compound libraries for effects o

228 Using an insect cell-based screening platform, we identified and charact

229 lutionizing the conventional high-throughput cell-based screening system, its limitation is obvious,

230 the Hh-pathway modulator Pipinib by means of cell-based screening.

231 ased standard dose (SD) quadrivalent (IIV4), cell-based SD quadrivalent (cIIV4), and recombinant quad

232 t on the development of rapid, point of need cell based sensing with boosted sensitivity and wide rel

233 ctionalities could revolutionize fields like cell-based sensing and create diverse new cellular pheno

234 The proposed optogenetically modulated cell-based sensing platform is suitable for in field app

235 Cell-based sensing platforms provide functional informat

236 significant advance against state of the art cell based sensors.

237 action was confirmed by in vitro binding and cell-based signaling assays.

238 Red blood cell-based solutions, artificial hemoglobin solutions, a

239 Using our novel stem cell-based spermatogenesis model, we show that PBB153 ex

240 le-, nucleic acid-, peptide-, antibody-, and cell-based strategies.

241 further apply this chemical biology tool to cell-based studies on the role of a sulfation site in th

242 replication with no significant toxicity in cell-based studies.

243 In the present study, using a cell-based system, we investigated whether ALK regulates

244 production parameters are hard to achieve in cell-based systems because of the need to maintain life

245 this is precluded by limitations of current cell-based systems for monitoring flavivirus infection i

246 L1 biology as elucidated from experiments in cell-based systems, cell-free systems, mouse and lower o

247 is, molecular modeling, and in cell-free and cell-based systems, we demonstrate that substitutions of

248 ms and highlight the potential of novel stem cell-based T2D disease models.

249 mpounds that inhibit tau pathology: a HEK293 cell-based tau overexpression assay, and a primary rat c

250 Screening 23dd in a cell-based TCF/LEF reporter gene assay restored the acti

251 sphoflow cytometry (phosphoflow) is a single-cell-based technique to measure phosphorylated intracell

252 tracellular chloride ion channels using live-cell based techniques, such as patch-clamp electrophysio

253 has led to a need for the next generation of cell-based technologies that enable both the collection

254 With the advent of single-cell-based technologies, a new 'worldview' is emerging:

255 rategy to improve the yield of HSCs for stem cell-based therapeutic agents.

256 s and may catalyze the discovery of new stem-cell-based therapeutic approaches.

257 gene expression using both pharmacologic and cell-based therapeutic approaches.

258 in the development of the next generation T-cell based therapeutics.

259 h as CRISPR/Cas9 in the manufacture of novel cell-based therapeutics has the potential to enable high

260 ts, laying the framework for optimization in cell-based therapeutics intended to mitigate cardiomyopa

261 c results that could provide new options for cell-based therapeutics with curative intent.

262 attempts and describe the updated status of cell-based therapies aimed at counteracting the skeletal

263 Cell-based therapies aimed at replenishing renal parench

264 supporting a therapeutic role for vaccines, cell-based therapies and immune-checkpoint inhibitors an

265 Cell-based therapies and pre-vascularized tissues have s

266 Cell-based therapies are gaining prominence in treating

267 Cell-based therapies are promising treatments for variou

268 ncer cells to induce tolerance to allogeneic cell-based therapies by modifying cells to express immun

269 of Th17 immunity, and open new avenues for T cell-based therapies for Nod2-associated disorders such

270 epair and may serve as inspiration for novel cell-based therapies for skeletal pathologies, such as o

271 development and may help guide the design of cell-based therapies for treating retinal dystrophies.

272 Cell-based therapies in muscular dystrophies have been p

273 hMSCs) are a promising source for engineered cell-based therapies in which genetic engineering could

274 l expansion may improve the efficacy of stem cell-based therapies targeting fibrosis.

275 s thus provide a rationale for developing NK cell-based therapies to effectively treat MYC-driven lym

276 ation is a cornerstone in manufacturing of T cell-based therapies, and precise control over T cell ac

277 ches is ushering in a new generation of stem cell-based therapies, greatly expanding their therapeuti

278 Cell-based therapies, with or without genetic modificati

279 itinol thin films can improve the effects of cell-based therapies.

280 d models of mtDNA disease and support future cell-based therapies.

281 zation process can be used to develop robust cell-based therapy against novel disease targets.

282 esource for personalized vaccine or adoptive cell-based therapy development.

283 ve received less attention as an alternative cell-based therapy for animals or even humans.

284 tem cell-derived interneurons is a promising cell-based therapy for treatment of these disorders.

285 tential in vitro, a major limitation for the cell-based therapy of liver disorders and for ex vivo bi

286 at include engineering biological valves and cell-based therapy strategies to replace coronary vascul

287 hence, they might be considered for further cell-based therapy study on wound healing.

288 so large-scale applications and personalized cell-based therapy.

289 ere we developed an induced pluripotent stem cell-based three-dimensional model that recapitulates an

290 heir biological roles and potential for stem cell-based tissue engineering approaches have not been c

291 our study developed a selective and scalable cell-based tool to investigate mHTT seeding activities i

292 To address this question, a new cell-based trans-activation assay system suitable for ZI

293 Single-cell-based transcriptome analyses revealed that skeletal

294 (HLA) bound peptides capable of mediating T cell-based tumor rejection still face important challeng

295 and under various conditions (cell-free and cell-based, two cell types).

296 s, such as decisions on their inclusion in T cell-based vaccines.

297 be useful in the rapid design of effective T cell-based vaccines.

298 sed by viral escape for the development of T cell-based vaccines.

299 s is essential for the design of effective T cell-based vaccines.

300 enables comparable biocatalytic activity to cell-based workflows in almost half the time.