ライフサイエンスコーパス: cellulase

1 and Ab/aptamer reporter molecules linked to cellulase.

2 degrees C) without or with hemicellulase or cellulase.

3 completion without expression of a family 48 cellulase.

4 pectin methylesterase, polygalacturonase and cellulase.

5 e reaction after nitrocellulose digestion by cellulase.

6 ter removing mucilage using the xylanase and cellulase.

7 ere able to strongly enhance the activity of cellulases.

8 n cellulosome chimeras with key cellulosomal cellulases.

9 nding module from CipA in complex with Cel8A cellulases.

10 monooxygenases that enhance the activity of cellulases.

11 lly, which confirmed that these proteins are cellulases.

12 Family 6 cellulases compared to GH Family 7 cellulases.

13 to computationally distinguish them from non-cellulases.

14 ntly increases product binding to processive cellulases.

15 ulases much more strongly than nonprocessive cellulases.

16 used to screen metagenomics data for "true" cellulases.

17 can be accomplished by fungal and bacterial cellulases.

18 re optimal for activity of commercial fungal cellulases.

19 ganism despite the presence of several other cellulases.

20 ollection contains hundreds of highly stable cellulases.

21 icyanide, on nitrocellulose films treated by cellulases.

22 vated temperatures, unlike commercial fungal cellulases.

23 om the performance barriers affecting fungal cellulases.

24 trategy that utilizes multi-catalytic domain cellulases.

25 of a range of glycoside hydrolases including cellulases.

26 t information for the selection of technical cellulases.

27 (6 and 10ppm), xylanase (70 and 120ppm) and cellulase (35 and 60ppm) on the rheological properties o

28 ld (369.23+/-0.11mug) in 1h in comparison to cellulase (359+/-0.30mug) and pectinases (333+/-0.55mug)

29 Among the 25 enzymes, 10 cellulases, 4 xylanases, 3 mannanases, 2 xyloglucanases,

30 ctrochemical assay for the total activity of cellulase, a hydrolytic enzyme widely used in food and t

31 f cellulose synthesis in Archaeplastida, and cellulases absent in terrestrial plants as well as the o

32 a comprehensive kinetic model for processive cellulases acting on insoluble substrates to explain thi

33 yet this approach has not been utilized for cellulases acting on their natural substrate, insoluble

34 A key component of cellulase action on cellulose is product inhibition from

35 Product inhibition negatively impacts cellulase action, but experimental measurements of produ

36 ccurring on the cellulosic substrate through cellulase action.

37 n deconstruction are essential to understand cellulase action.

38 Cellulases active above 100 degrees C may assist in biof

39 Numerical values of cellulase activities measured with the QCM method showed

40 Trichoderma and Aspergillus niger cellulases activities were determined in a 5 min assay w

41 ited a more generalist phenotype with higher cellulase activity and growth capabilities on different

42 Purification of the soluble cellulase activity from a 300litre cultivation of this c

43 Increased cellulase activity is achieved in stress environments (s

44 nments (switching carbon sources), otherwise cellulase activity is minimized to conserve ATP.

45 eans of inducing an organism-level change in cellulase activity is to use laboratory adaptive evoluti

46 The enhancement in apparent cellulase activity was attributed to the "amorphous-like

47 Cellulase activity was detected on CMC all along the len

48 A clone encoding carboxymethyl cellulase activity was isolated during functional screen

49 The three with the highest cellulase activity were Actinosynnema mirum, Cellulomona

50 ourse of evolution concurrent with decreased cellulase activity, increased intracellular ATP concentr

51 to cellulose is known to be proportional to cellulase activity, such that increasing binding affinit

52 Although linker modifications can alter cellulase activity, the functional role of linkers beyon

53 rils to elucidate molecular level details of cellulase activity.

54 nd eight of the organisms showed evidence of cellulase activity.

55 es balance ATP energetic considerations with cellulase activity.

56 ring growth on crystalline cellulose and for cellulase activity.

57 ciated with increased ratios of ligninase to cellulase activity.

58 provide insights into the initial events of cellulase adsorption and diffusion on cellulose.

59 Despite numerous studies documenting cellulase adsorption to lignin, few attempts have been m

60 r cellulosomes alongside an endo- and an exo-cellulase also converted to the cellulosomal mode.

61 Supplementation to cellulases also resulted in an increased cellulose hydro

62 loped by pre-treating the stevia leaves with cellulase and adding soluble starch as the glucosyl dono

63 he number in proteins from bran treated with Cellulase and digested with the same protease.

64 e of 36-45min and the cocktail of pectinase, cellulase and hemicellulase, set at 2% each, gave the be

65 iments are presented that utilize commercial cellulase and laccase enzymes, which are known to modify

66 er, the presence of xylanase, alpha-amylase, cellulase and lipase resulted in bread with greater quan

67 hing to a catalytic site template from a GH9 cellulase and other analyses point to a putative catalyt

68 rs, agitation, liquid/solid (L/S) ratio, and cellulase and protease addition, on extraction yield of

69 t response, including induction of all major cellulase and some major hemicellulase genes.

70 xtraction by using three enzymes (pectinase, cellulase and tannase) and an enzymatic blend to increas

71 ndent variables: temperature, pH, pectinase, cellulase and tannase; and a subsequent optimization thr

72 C. chrysophilum showed the highest cellulase and the lowest lipase activity, while C. noveb

73 t extracted with carbohydrases, Viscozyme or Cellulase and then hydrolyzed with proteases (Alcalase,

74 Enzymatic extractions with Cellulase and Viscozyme were responsible for highest ext

75 Cellulase and xylanase under five different combinations

76 60 most upregulated ones that comprised two cellulases and a cellulose synthase was conserved among

77 In T. fusca, cellulases and a putative cellobiose ABC transporter are

78 e enzymes enhance the hydrolytic activity of cellulases and are essential for cost-efficient conversi

79 is achieved by the action of a wide range of cellulases and associated enzymes.

80 s identified structural elements that define cellulases and can be used to computationally distinguis

81 and, despite the growth limitation, produced cellulases and degraded cellulose more efficiently.

82 al methods are unable to distinguish between cellulases and enzymes with different substrate specific

83 ymes in buffalo rumen metagenome and that of cellulases and hemicellulases in termite hindgut was obs

84 be used for in silico discrimination between cellulases and non-cellulases belonging to GH48 is an om

85 approach supported by experimental studies, cellulases and non-cellulases can be effectively identif

86 work focuses both on improved attributes of cellulases and on the domains of cellulase that have bee

87 robes including Clostridium species organize cellulases and other glycosyl hydrolases into large comp

88 Here, we explore the diversity of cellulases and propose a genomic approach to overcome th

89 repancies reported for binding constants for cellulases and suggest that product inhibition will vary

90 The model is applicable to individual cellulases and their mixtures at low-to-medium enzyme lo

91 ability by imaging the distribution of major cellulases and xylanases in Trichoderma reesei using the

92 studied on a panel of relevant glycosidases (cellulases and xylanases) in microtiter plates.

93 All of these approaches involve multiple cellulases and, since cellulose is insoluble and microor

94 y Eng-1, Cathepsin S-like cysteine protease, cellulase, and two unknown genes with secretory characte

95 to dabsyl-tagged enzyme substrates to screen cellulases, and for the analysis of plant cell wall hemi

96 ies on the consortium, a 90 kDa, multidomain cellulase, annotated as a member of the TIM barrel glyco

97 Secreted mixtures of Hypocrea jecorina cellulases are able to efficiently degrade cellulosic bi

98 lternatively, in certain anaerobic microbes, cellulases are assembled into large multienzymes complex

99 some, CelR also regulates cellulases, while cellulases are controlled by different regulatory sites

100 Bacterial GH Family 6 cellulases are found with structured domains in either N

101 microorganisms cannot ingest particles, the cellulases are present outside of the cell although they

102 In aerobic microrganisms, cellulases are secreted as free enzymes.

103 Glycoside hydrolase family 7 (GH7) cellulases are some of the most efficient degraders of c

104 However, the reported koff values of cellulases are strongly dependent on the method used for

105 Cellulose-degrading enzymes (cellulases) are often modular, with catalytic domains fo

106 Our data thus identify CelC2 cellulase as an important determinant of events underlyi

107 nzymatically responsive to both nuclease and cellulase, as well as small molecules, showing great pot

108 the basis of its ease of use, we expect this cellulase assay platform to be applicable to enzyme scre

109 iently degrades beta-1,4-glucans in in vitro cellulase assays with carboxymethyl-cellulose as substra

110 In contrast, the most tolerant cellulase behaves similarly in water and in ionic-liquid

111 co discrimination between cellulases and non-cellulases belonging to GH48 is an omega-loop located on

112 These symbionts lack cellulases but encode a distinctive and lineage-specific

113 tion of expansins for cellulose digestion by cellulases, but only rarely to an extent that is commerc

114 wer binding partition coefficient for fungal cellulases, but surprisingly, it enhanced hydrolytic act

115 duct binding on processive and nonprocessive cellulases by calculating the binding free energy of cel

116 lucose and alleviate the inhibition of other cellulases by cellobiose during saccharification.

117 ost cellulolytic bacteria have one family 48 cellulase, C. thermocellum has two, Cel48S and Cel48Y.

118 esults show for the first time that a single cellulase can be essential for cellulose degradation by

119 y of the extracellular enzymes ligninase and cellulase can be used to track changes in the predominan

120 at highly homogeneous populations of labeled cellulases can be achieved.

121 by experimental studies, cellulases and non-cellulases can be effectively identified within a given

122 Cellulose is the main polymer in biomass and cellulases can hydrolyze it to cellobiose, which can be

123 Cellobiohydrolases (CBHs) are cellulases capable of liberating many sugar molecules in

124 lose) and 60-70% lower maximum surface-bound cellulase capacity.

125 e find that the two most abundantly secreted cellulases, CBH-1 and CBH-2, depend on distinct ER cargo

126 s a suite of enzymes including endo- and exo-cellulases, CBM33 polysaccharide-monooxygenases, and hem

127 e binding mode, Doc124A directs the appended cellulase, Cel124A, to the surface of C. thermocellum an

128 ynergistic GH9 with the major endoprocessive cellulase Cel48F, but also identify Cel9U as an importan

129 yptophans in the Trichoderma reesei family 6 cellulase (Cel6A) to alanine.

130 exation and decomplexation of the processive cellulase Cel7A and its insoluble substrate, cellulose.

131 e pre-steady-state regime for the exo-acting cellulase Cel7A using amperometric biosensors and an exp

132 the bacterial cellulase CelA with the fungal cellulase Cel7A.

133 equence is 78% identical to the cellulosomal cellulase Cel9E, was found inactive in the free and comp

134 g the cellulolytic activity of the bacterial cellulase CelA with the fungal cellulase Cel7A.

135 CBM) 4 Ig fused domain from the cellulosomal cellulase cellobiohydrolase A (CbhA) of Clostridium ther

136 mbination enables quantitative prediction of cellulase chimera thermostability and efficient identifi

137 nt markedly enhanced potential activities of cellulase (CL), beta-glucosidase (BG), lignin peroxidase

138 ands are disrupted by water in nonprocessive cellulase clefts, and the lack of long tunnel-forming lo

139 The activity of a cellulase cocktail is clearly evident through the TOF-SI

140 sLac acted synergistically with a commercial cellulase cocktail to increase glucose production from S

141 The inhibitory action of lignin on cellulase cocktails is a major challenge to the biologic

142 A prominent example is the cellulase cocktails secreted by fungi and bacteria for d

143 could potentially be used to design improved cellulase cocktails, thus lowering the overall cost of b

144 of the biochemically characterized bacterial cellulases come from only a few cellulose-degrading bact

145 nkers are observed in eukaryotic GH Family 6 cellulases compared to GH Family 7 cellulases.

146 y hot-air, far-infrared radiation (FIR), and cellulase, compared with raw samples.

147 ellulovorans' multidomain GHs assembled into cellulase complexes through glycosylation.

148 Cellulases comprise many glycoside hydrolase families an

149 Modular cellulases contain non-catalytic type A carbohydrate-bin

150 which requires neither an edible plant nor a cellulase, could enable crude biomass to be the sole sou

151 onstrate that the small, noncomplexed fungal cellulases deconstruct cell walls using mechanisms that

152 Acid pre-treatment and cellulase digestion released significantly more sugars f

153 Following cellulase digestion, we measured a significant decrease

154 The sole noncellulosomal GH9 (Cel9W) is a cellulase displaying a broad substrate specificity, whos

155 nt within and between twins (e.g., predicted cellulases, dockerins), and transcriptional activities.

156 less severe pretreatment and 300-400% lower cellulase dosages for equivalent product yields using si

157 Understanding the enzymatic mechanism that cellulases employ to degrade cellulose is critical to ef

158 mising approach involves the application of "cellulase-enhancing factors," such as those from the gly

159 Using a cellulase enzyme (Celluclast(R) 1.5L), different paramet

160 -29 cargo adaptors, as well as their role in cellulase enzyme trafficking.

161 enesis to validate these findings on a model cellulase enzyme, an endoglucanase from the thermophilic

162 Cellulase enzymes cleave glycosidic bonds in cellulose t

163 Cellulase enzymes deconstruct cellulose to glucose, and

164 Cellulase enzymes deconstruct recalcitrant cellulose int

165 errogate the requirements for trafficking of cellulase enzymes from the endoplasmic reticulum to the

166 , in which polysaccharides are hydrolyzed by cellulase enzymes into simple sugars and fermented to et

167 Cellulase enzymes often contain carbohydrate-binding mod

168 Currently, the cost of cellulase enzymes remains a key economic impediment to c

169 he fungus Trichoderma reesei, which secretes cellulase enzymes to hydrolyze lignocellulosic biomass i

170 enzymatic digestion reveals that processive cellulases exploit these defects as initiation sites for

171 enome scale for diverse phenotypes including cellulase expression, isobutanol production, glycerol ut

172 Flavourzyme and Cellulase extracts are richer in macro (Mg, K and P) and

173 was observed in ultrasound, Flavourzyme and Cellulase extracts.

174 ological perspective due to the diversity of cellulase families, their unique assembly and substrate

175 ves, GH74 was originally considered to be a "cellulase" family, although more recent studies have gen

176 strains may significantly improve yields of cellulases for cellulosic biofuel production.

177 opment of novel pretreatments and engineered cellulases for cost-effective biofuels production.

178 eatly facilitate the engineering of improved cellulases for the large-scale conversion of plant bioma

179 es, and structure of a unique endogenous GH7 cellulase from an animal, the marine wood borer Limnoria

180 We have demonstrated that the CelA cellulase from Caldicellulosiruptor bescii degrades high

181 erma reesei ; thus it can be used to compare cellulases from different organisms.

182 cific genomic locus that encodes multidomain cellulases from GH families 9 and 48, which are associat

183 aken together, Dpi protects key cellulosomal cellulases from proteolysis in H10.

184 quid tolerance among three distinct family 5 cellulases from Trichoderma viride, Thermogata maritima,

185 Many fungal cellulases function optimally at pH ~5, and their activi

186 lucosidase activity, but efficiently induces cellulase gene expression and cellulolytic activity in t

187 of the clr-2 homolog (clrB) failed to induce cellulase gene expression and lacked cellulolytic activi

188 porters (Delta3betaGDelta2T) does not induce cellulase gene expression in response to cellobiose.

189 ns for both transporters is unable to induce cellulase gene expression in response to crystalline cel

190 lucosidase activity, but efficiently induces cellulase gene expression in the presence of cellobiose,

191 Thus, the ability to induce cellulase gene expression using a common and soluble car

192 o not greatly impact cellobiose induction of cellulase gene expression.

193 ce of an inducer is not sufficient to induce cellulase gene expression.

194 y shows that deletion of the single family 9 cellulase gene in Clostridium phytofermentans prevents g

195 rows perfectly well on glucose and its other cellulase genes are transcribed normally.

196 argeted gene disruptions of all 13 predicted cellulase genes showed that only cel5B and cel6A were re

197 verify the importance of a highly expressed cellulase (GH6 family cellobiohydrolase) and the CebR tr

198 , combination of alpha-amylase, xylanase and cellulase had a synergetic effect on the dough rheology.

199 A new method to determine the activity of cellulase has been developed using a quartz crystal micr

200 The intrinsic processivity (P(Intr)) of cellulases has been shown to be governed by the rate con

201 3 fungal class II cellobiohydrolases (CBH II cellulases) has yielded a collection of highly thermosta

202 Cellulases, hemicellulases including mannanases and othe

203 catalytic subunits containing cell-adherent cellulases, hemicellulases, xylanases, and other glycosi

204 Cellulases hydrolyze beta-1,4 glycosidic linkages in cel

205 which we show coincided with a radiation of cellulases implicated in cell division.

206 udies determined the role of rhizobial CelC2 cellulase in different steps of the symbiotic interactio

207 trates, and different synergies with pivotal cellulases in mini-cellulosomes.

208 e measured hydrolytic rates of four purified cellulases in small increments of temperature (10-50 deg

209 Finally, incorporation of all GH9 cellulases in trivalent cellulosome chimera containing C

210 f all ESTs represent genes encoding putative cellulases, including glycosyl hydrolase family 7 (GH7)

211 Agitation and addition of cellulases increased oil extraction yield, indicating th

212 However, alpha-amylase and cellulase incubation caused significant increases in the

213 ontrast Cel12A, a bifunctional xyloglucanase/cellulase, induced creep with only subtle changes in wal

214 leads to the conclusion that synergism among cellulases is morphology-dependent and governed by the c

215 drolysis and the synergistic interplay among cellulases is yet poorly understood.

216 The cellulase-labeled immuno-aptamer sandwich applied onto n

217 Here, an inexpensive cellulase-linked immunomagnetic methodology was develope

218 the need for additional studies to elucidate cellulase linker functions.

219 Here we investigate cellulase linkers connecting GH Family 6 or 7 catalytic

220 This study suggests that cellulase linkers may exhibit function in enzyme action,

221 tive N-glycosylation sites are quite rare in cellulase linkers, while an N-P motif, which strongly di

222 ts to linkers are relevant for understanding cellulase mechanisms.

223 h known activities comprising (carboxymethyl)cellulases, mixed-linkage endo-glucanases, and endo-xylo

224 at enhanced levels of HjCel3A in H. jecorina cellulase mixtures benefit the conversion of cellulose t

225 those of lower crystallinity indices for the cellulase mixtures examined.

226 Cellulase mixtures from Hypocrea jecorina are commonly u

227 LPMOs that are present in current commercial cellulase mixtures in part is due to hitherto undetected

228 s between hydrolysis with endoglucanases and cellulase mixtures were observed.

229 w covers the topic of protein engineering of cellulases, mostly after 2009.

230 suggest that cellobiose binds to processive cellulases much more strongly than nonprocessive cellula

231 Overall, this study suggests that marine cellulases offer significant potential for utilization i

232 ving the coordinated expression of different cellulases, often in a synergistic manner.

233 The immobilization of cellulase on amine-functionalized Fe(3)O(4) magnetic nan

234 structural characterization of the action of cellulases on a nano-flat cellulose preparation, which e

235 ces higher concentrations of secreted active cellulases on cellobiose.

236 amount of productive binding for processive cellulases on cellulose.

237 three enzymes; alpha-amylase, pectinase and cellulase onto amino-functionalized magnetic nanoparticl

238 proach to be amenable to thermophile-derived cellulases or to the separation of multiple species usin

239 Phytase + xylanase + cellulase (P + X + C) treatment increased iron bioaccess

240 tevioside from Stevia rebaudiana leaves with cellulase, pectinase and hemicellulase, using various pa

241 inations of solvent and enzyme, enzyme type (cellulase, pectinase, ss-glucosidase), and hydrolysis ti

242 nd may thus be a general strategy to enhance cellulase performance.

243 Also, ethanol extract of cellulase pre-treated ginger had the maximum polyphenol

244 for reliable computational identification of cellulases precludes their exploration in the genomic da

245 surface ablation mechanism driven by general cellulase processivity, but also excavates extensive cav

246 To provide molecular level insights into cellulase product inhibition, we examine the impact of p

247 ngineering of filamentous fungi for improved cellulase production is hampered by our incomplete knowl

248 r in related ascomycetes used for industrial cellulase production.

249 of application of alpha-amylase, viscozyme, cellulase, protease and pectinase enzymes to ginger on t

250 Similarly, the addition of cellulase, protease, lipase and glucose-oxidase did not

251 Both the development and the application of cellulases require an understanding of the activities of

252 Furthermore, the free and immobilized cellulases retained 70 and 84% of their initial activity

253 Cellulase significantly increased the amount of vanillic

254 Despite the wealth of knowledge about cellulase structure and function, the elusive mechanism

255 nd the molecular-level implications of pH in cellulase structure, we use a hybrid, solvent-based, con

256 ty in hydrolytic activity found in different cellulase-substrate systems.

257 anization resembles the one adopted by other cellulases (such as cellobiohydrolases, for example) tha

258 to biofuels is dependent on highly efficient cellulase systems that produce near-quantitative levels

259 endoglucanase (EG), the major components of cellulase systems, take on distinct roles: EG and CBH II

260 Similar to other fungal and bacterial cellulase systems, the multienzyme cellulosome system of

261 quent digestion by two commercially relevant cellulase systems.

262 The immobilized cellulase (tested by carboxymethyl cellulose hydrolysis

263 tributes of cellulases and on the domains of cellulase that have been improved.

264 n intermediate strategy, secreting many free cellulases that contain multiple catalytic domains.

265 aori: to join), not directly associated with cellulases, that mediate attachment to cellulose by spec

266 atically (beta-glucuronidase/arylsulphatase, cellulase), the compounds separated on a reversed phase

267 The surface-disrupting activity of cellulases therefore strongly depends on mesoscopic stru

268 allinity substrates making it the only known cellulase to function well on highly crystalline cellulo

269 or the prevention of unproductive binding of cellulase to the biomass lignin.

270 ondition obtained was: liquid to solid (LS), cellulase to xylanase and enzymes to matter ratios of 22

271 y, we show that prior action of LPMO enables cellulases to attack otherwise highly resistant crystall

272 r 2 CBMs, from both bacterial and eukaryotic cellulases to identify conserved characteristics potenti

273 ic constant that reflects the ability of the cellulases to overcome substrate recalcitrance.

274 The contents of gamma-oryzanol in cellulase treated ground rice husk were significantly in

275 alpha- and gamma-tocopherols in hot-air and cellulase treated rice bran were remained unchanged.

276 io-active constituents in the oleoresin from cellulase-treated ginger.

277 n of the lignocellulosic residue followed by cellulase treatment and conversion to ethanol at a high

278 In grape pomace, cellulase treatment was not efficient for phenolic relea

279 with the sensitivity of their directions to cellulase treatment, indicates they most likely reflect

280 nitrocellulose film and is evoked after the cellulase treatment.

281 phenolic content (TPC) than did hot-air and cellulase treatments for all samples.

282 ide and disaccharides in the product site of cellulase tunnel.

283 and and the cellobiose product in processive cellulase tunnels and the additional stabilization from

284 Components of modular cellulases, type-A cellulose-binding modules (CBMs) bind

285 to D-lactate (50 degrees C and pH 5.0), the cellulase usage could be reduced to 1/3 that required fo

286 of the structural and dynamic features that cellulases utilize to bind a single strand of crystallin

287 demonstrate how the synergistic activity of cellulases was enhanced by altering the hydrogen bond ne

288 lexin produced in response to elicitation by cellulase, was investigated.

289 Gal), endo-1,4-beta-D-glucanase (EGase), and cellulase were monitored during growth and ripening of S

290 Simulations of the three cellulases were conducted at a range of temperatures in

291 o effect on product binding in nonprocessive cellulases, whereas it significantly increases product b

292 In some, CelR also regulates cellulases, while cellulases are controlled by different

293 ndustrially important GH Family 7 processive cellulases with free energy perturbation/replica-exchang

294 are the Cel7A linker with linkers from other cellulases with sequence-based tools to predict disorder

295 ulose-binding proteins and is applied to tag cellulases with three different fluorophores.

296 Family 7 enzymes from other genera and other cellulases within T. reesei may not be as disordered, wa

297 nd a 2.6-fold enhancement compared with free cellulases without LPMO enhancement.

298 Our engineered strains express cellulase, xylanase, beta-glucosidase, and xylobiosidase

299 med the biochemical activities of a GH5-GH10 cellulase-xylanase and a GH11-CE4 xylanase-esterase.

300 xylanase under five different combinations (cellulase/xylanase: 50/0, 50/25, 50/50, 25/50, and 0/50