ライフサイエンスコーパス: centrifugation

1 med "oncosomes") preisolated by differential centrifugation.

2 action (CRF) was recovered from the juice by centrifugation.

3 eriments, and are typically achieved through centrifugation.

4 from healthy volunteers (n=4) by sequential centrifugation.

5 was used to induce emulsion breaking without centrifugation.

6 is unchanged compared to plasma obtained via centrifugation.

7 ved exosomes (EXOs) isolated by differential centrifugation.

8 ated from culture supernatants by sequential centrifugation.

9 urine samples through multistep differential centrifugation.

10 performed by native PAGE and sucrose density centrifugation.

11 or either (b) one round or (c) two rounds of centrifugation.

12 Insoluble debris was removed by centrifugation.

13 particles, and their ordering is improved by centrifugation.

14 n virion proteins following sucrose gradient centrifugation.

15 sions by MS while circumventing dialysis and centrifugation.

16 Mouse BMCs were isolated by density-gradient centrifugation.

17 ies would normally be removed by dialysis or centrifugation.

18 plasma from whole blood without the need for centrifugation.

19 ted into cellular and acellular fractions by centrifugation.

20 anical pressing, heating to 50 degrees C and centrifugation.

21 ample preparation such as filtration step or centrifugation.

22 which protein was separated from fat through centrifugation.

23 of the liver and subsequent density-gradient centrifugation.

24 t biomarkers that could be obtained at lower centrifugation.

25 s with electrostatically assisted high-speed centrifugation.

26 cteria were concentrated by density gradient centrifugation.

27 of reaction product, enabling separation by centrifugation.

28 of trichloroacetic acid (TCA) and subsequent centrifugation.

29 conditions and overcome sample filtration or centrifugation.

30 further separation of both liquid phases by centrifugation.

31 further and cell fractions are separated by centrifugation.

32 cted in the bottom of the tubes under gentle centrifugation.

33 rably faster than with conventional gradient centrifugation.

34 nding arc-discharge SWNTs with I followed by centrifugation.

35 via collection of the SPIONs by either F4 or centrifugation.

36 the reaction mixture by simple filtration or centrifugation.

37 f platelet-rich plasma to about 6.5 prior to centrifugation.

38 ant female donors and plasma separated using centrifugation.

39 field-flow fraction (F4) in combination with centrifugation.

40 able saving of time and removes the need for centrifugation.

41 on their separation through sucrose gradient centrifugation.

42 ing ultracentrifugation and sucrose gradient centrifugation.

43 d placed on ice for transport to the lab for centrifugation.

44 binding to lipids in liposomes using density centrifugation.

45 recipitated using 4.0 M Lithium Chloride and centrifugation.

46 epared from corresponding plasma obtained by centrifugation.

47 ng experienced health-care professionals and centrifugation.

48 be separated from the media with no need to centrifugation.

49 ion of an apoplast wash solution followed by centrifugation.

50 eric micelles together with purification via centrifugation.

51 ibia, compared with artificial 1G induced by centrifugation.

52 ry of its chloroplast-rich fraction (CRF) by centrifugation.

53 e colloidal complexes were recovered through centrifugation.

54 g a sequence of extraction with solvents and centrifugation.

55 lls were enriched by Ficoll density gradient centrifugation.

56 thod efficiently recovered data with delayed centrifugation.

57 is, immunoprecipitation, and sucrose density centrifugation.

58 cle fractions from differential and gradient centrifugations.

59 y purified from mature seeds by differential centrifugations.

60 After centrifugation, 1 muL of the sedimented phase was analys

61 B were heavy and readily settled down under centrifugation (13,000rpm, 60min).

62 y in which 5 ml of plasma is concentrated by centrifugation (1x, concentrated).

63 with sodium sulfide following high-velocity centrifugation (21500g, 45 min) at 4 degrees C.

64 o laser engineered micromoulds by high-speed centrifugation (30min) to concentrate NMP into MN shafts

65 es by Blue Native PAGE, Suc density gradient centrifugation, 77K fluorescence, circular dichroism, an

66 The final biphasic system was separated by centrifugation (8 min, 10,000 rpm).

67 After centrifugation, 97% Cd(II) was removed from the cocultur

68 nducted without manual operations, including centrifugation, a widely used means of isolating a solid

69 heres can be easily removed by filtration or centrifugation after extraction reaction.

70 is useful to remove these nanomaterials via centrifugation after reacting with spin trapping agents.

71 sors can be readily removed by filtration or centrifugation after use, thus making it a convenient to

72 We find that while differential centrifugation alone gives a first approximation of the

73 iple rounds of differential density gradient centrifugation and analyzed by immunoelectron microscopy

74 developed and validated based on systematic centrifugation and comparison of colony forming unit (CF

75 tage, followed by a subsequent sonification, centrifugation and concentration step.

76 size and zeta-potential were prepared using centrifugation and consequently employed for BOD-ErGO bi

77 d lipids with acetonitrile:water (3:2, v/v), centrifugation and continuous solid-phase extraction for

78 of raw milk, which only involves incubation, centrifugation and dilution steps.

79 Instead, sucrose density gradient centrifugation and electron microscopy showed that BFA a

80 the AAVS1 site, we show by using analytical centrifugation and electron microscopy that the full-len

81 maize Golgi membranes isolated by flotation centrifugation and enriched further by free-flow electro

82 es were pretreated by protein precipitation, centrifugation and filtration, followed by HPLC separati

83 Compared with traditional methods based on centrifugation and filtration, the established method is

84 re isolated from Giardia by density gradient centrifugation and found to be sensitive to nystatin and

85 modal particle size populations isolated via centrifugation and immune-affinity methods respectively.

86 the detection of NS1 in plasma obtained via centrifugation and in plasma separated on-chip.

87 Consistent with these data, sucrose gradient centrifugation and indirect immunofluorescence microscop

88 e UvsY heptamer was confirmed in solution by centrifugation and light scattering, and thermodynamic a

89 as examined in ES cell lines by differential centrifugation and membrane solubilization by digitonin.

90 s of different accessions; 2) the effects of centrifugation and microwave treatment on extracting oil

91 The combined use of centrifugation and microwave treatments gave high oil ex

92 Combining gradient centrifugation and Mito-AP techniques resulted in high p

93 This film, applied by centrifugation and modified in high-frequency discharge

94 mically large structures in density gradient centrifugation and native gel electrophoresis.

95 astringency removal, flash vacuum expansion, centrifugation and pasteurization) in order to evaluate

96 and centrifugation, and astringency removal, centrifugation and pasteurization, respectively) contain

97 ng two methods for EV recovery, differential centrifugation and polyethylene glycol precipitation fol

98 t pre-treatment of the samples, e.g. without centrifugation and precipitation of protein with acids.

99 samples extracted by two different methods (centrifugation and pressed processing) were characterize

100 In conclusion, this method does not require centrifugation and produces a clean spectrum for VMS ana

101 le murine and human neural tissues by serial centrifugation and purification on a sucrose gradient.

102 ecialized vials by a phlebotomist along with centrifugation and refrigerated storage.

103 t rely on precipitation of a urine pellet by centrifugation and requires a smaller volume of urine.

104 CAS caused a rapid ATPS without resorting to centrifugation and salt, mainly because of strong electr

105 s in urine were analyzed by fractionation by centrifugation and separation by sodium dodecyl sulfate

106 ere used; six honey samples were obtained by centrifugation and six by honeycomb press.

107 Crude peptides were separated by high-speed centrifugation and tested for antioxidant and antimutage

108 ntly separated using a sequential process of centrifugation and then detected using an immobilized go

109 Here, we used sucrose gradient centrifugation and transiently transfected HEK 293T cell

110 rom the digested extracts was carried out by centrifugation and ultrafiltration.

111 cols to fulfill these tasks require multiple centrifugation and vacuum-centrifugation steps, making l

112 ting microstructure during storage and after centrifugation and washing was investigated.

113 ellar genomes and metabolites are removed by centrifugation and washing, thus maximizing the utility

114 8% at pH 3 exhibited intact structures after centrifugation and washing.

115 t in a couple of hours without requiring any centrifugation and/or vacuum centrifugation steps.

116 Combined physical (centrifugation) and chemical (molybdate reactiveness) fr

117 droplet size following exposure to physical (centrifugation) and thermal stressors (freeze, thaw cycl

118 reated donor cells (Donor cells) using ultra-centrifugation, and analyzed for acetylcholinesterase ac

119 rgoing the combinations of clarification and centrifugation, and astringency removal, centrifugation

120 s studied through pressure filtration tests, centrifugation, and characterization of hard-to-remove (

121 c acid to release the protein-bound vitamin, centrifugation, and mixing of the supernatant with phosp

122 ere fractionated by Percoll density gradient centrifugation, and N- and O-glycans present in each com

123 structured-illumination microscopy, gradient centrifugation, and proteasome inhibition approaches rev

124 on as well as the aqueous phase separated by centrifugation, and quantification by absorption spectro

125 ltered sedimentation during sucrose gradient centrifugation, and retarded mobility during native agar

126 rger colloids prior to analysis (filtration, centrifugation, and sedimentation).

127 Live imaging of Hyal1, sucrose gradient centrifugation, and specific colocalization of Rab GTPas

128 The sample pretreatment only involved centrifugation, and the time required for lipid quantifi

129 nge style patch, extracted from the gauze by centrifugation, and then derivatized using CIL.

130 We have developed a novel blood lysis-centrifugation approach for highly sensitive Mycobacteri

131 The use of centrifugation as assistive technology appears to be nec

132 Here we show a centrifugation-assisted assembly of colloidal silica sph

133 e samples (down to 10 cells) without washing-centrifugation-assisted immiscible fluid filtration (CIF

134 d with ultra-high quality water and applying centrifugation-assisted ultrafiltration (CUF).

135 by homogenization at 15,000 rpm followed by centrifugation at 3000 rpm.

136 We subsequently incorporated a centrifugation-based fractionation step to deplete the h

137 lly compared it with the commonly used lysis-centrifugation-based purification method (STEM method) b

138 The technology replaces centrifugation-based separation with a faster and highly

139 A standard centrifugation-based water retention capacity, swelling

140 PBMCs were obtained by means of density centrifugation, basophils were purified with a specific

141 Through centrifugation, bilayer SnS nanosheets can be produced s

142 alphacat in the supernatant after high-speed centrifugation, but the protein still elutes in the void

143 tric concentration is typically achieved via centrifugation, but this technique is not well suited fo

144 ompare our device against a density gradient centrifugation by processing 0.5 mL portions of patient

145 rom the bulk of serum proteins by high-speed centrifugation causing substantial sample enrichment pri

146 cterized and stable under thermal-stress and centrifugation conditions.

147 The most robust assay involved an automated centrifugation-decantation step to remove latex aggregat

148 maller nanoparticles (diameter <150 nm), the centrifugation-decantation step was not required as the

149 Cross-linking sucrose density gradient centrifugation demonstrated an association of BC200 and

150 nce of soluble solid separated by high speed centrifugation denoted the presence of lycopene in this

151 be consistently isolated using differential centrifugation & density-gradient fractionation from bro

152 Centrifugation did not significantly improve ULTRA sensi

153 consists of a sequence of only three steps: centrifugation, dilution and filtration.

154 trast, genomic analysis of urine enriched by centrifugation discriminated between the groups, generat

155 tion maps can be obtained using differential centrifugation down to the suborganellar and protein com

156 nents were determined using a combination of centrifugation, drying, gas pycnometry and image analysi

157 ty to mediate fast diffusion of VX and a MAS centrifugation effect.

158 Rate-dependence of centrifugation efficiency for each component suggests di

159 ecovering the oil bodies from the extract by centrifugation, either in the presence of sucrose or by

160 to salivary gland debris by density gradient centrifugation eliminated salivary gland-associated inhi

161 Innovations such as centrifugation-enhanced shell vial and multiwell plate c

162 By combining sucrose gradient centrifugation experiments, limited proteolysis, one-dim

163 We relate, by simple analytical centrifugation experiments, the density of colloidal flu

164 Stopping the reaction with acetic acid and a centrifugation/filtration step to decrease turbidity are

165 on of the milk sample using acetonitrile and centrifugation followed by a solid-phase extraction (SPE

166 removed completely or to a trace level after centrifugation followed by filtration which minimises it

167 ia rapidly and inexpensively using low-speed centrifugation, followed by a single small-volume ultrac

168 acidified methanol, ultrasound treatment and centrifugation, followed by an immunoaffinity column (IA

169 entration, 65 samples underwent pelleting by centrifugation, followed by conventional Xpert MTB/RIF a

170 ated by nitrogen cavitation and differential centrifugation, followed by free flow electrophoresis.

171 (HCl or H2SO4), followed by separation using centrifugation for dissolved coagulant recovery.

172 lays markedly reduced in vitro dependence on centrifugation for infection and low incidence and sever

173 It relies on a simple but controllable centrifugation force for the actuation of samples and re

174 Furthermore, our data from differential centrifugation, Forster resonance energy transfer, nativ

175 es in Golgi membranes, enriched by flotation centrifugation from etiolated coleoptiles of maize (Zea

176 teria and EV from samples using differential centrifugation, genomic DNA was extracted and 16S-rDNA a

177 Centrifugation had no effect on DNA availability, wherea

178 However, common concentration by centrifugation has important limitations when processing

179 heating (i.e., manual) and by chromatography/centrifugation (i.e., column) methods.

180 sing in vivo crosslinking, velocity gradient centrifugation, immuno-isolation, and tandem mass spectr

181 Enzymatic assays, sucrose density gradient centrifugation, immunoprecipitation, dot and Western blo

182 Cell homogenization and sucrose gradient centrifugation in combination with transferrin-HRP/DAB a

183 parating dissociated liver cells by gradient centrifugation into two portions, hepatocytes (parenchym

184 ught into close proximity to target cells by centrifugation, intoxication was increased 4-fold.

185 atories to separate plasma from whole blood, centrifugation is generally inappropriate for on-site te

186 el underpinning the self-ordering process by centrifugation is proposed to describe the interplay bet

187 Percoll density centrifugation is simple, can be done on the day of tran

188 Centrifugation is the most common method used to enrich

189 are difficult to purify by density gradient centrifugation, leading to poor islet recovery.

190 Sputum centrifugation may increase the yield of Xpert MTB/RIF.

191 of each sample were processed by a new lysis-centrifugation method and tested with the Xpert MTB/RIF

192 is presented and compared to an established centrifugation method for analytics.

193 n capacity as determined by a novel drainage centrifugation method, and Farinograph absorption were n

194 Compared to the centrifugation method, use of the novel magnetic sulfate

195 oxins early by conducting multiple low-speed centrifugations, microfiltration, and cross-flow ultrafi

196 nd size of tubes used to produce PRF; and 6) centrifugation model used.

197 sample preparation (e.g., ultrafiltration or centrifugation) needed by more commonly employed techniq

198 mmon confounders such as age, BMI or time to centrifugation nor alternative methods to data normalisa

199 Following centrifugation, O-glycans are recovered in the filtrate.

200 D-Fuge devices presented here are capable of centrifugation of a wide variety of different solutions

201 es were observed by sucrose density gradient centrifugation of bacterial extracts during mid-logarith

202 bility was higher in subjects with immediate centrifugation of blood samples.

203 y polyhedrocyte formation from the forces of centrifugation of blood without clotting.

204 Centrifugation of buttermilk (5% total solids, TS)--curc

205 Density gradient centrifugation of isolated exosomes revealed the presenc

206 Density gradient centrifugation of L1 ribonucleoprotein particles, subcel

207 he volume of the pellet obtained by benchtop centrifugation of nanomaterial suspensions in a packed c

208 After acidification and centrifugation of pressure-treated solutions, purity of

209 by coating MPs with human ES cell lysate and centrifugation of specific ratios of ES cells and MPs to

210 ybeans; (ii) homogenisation, suspension, and centrifugation of the filter cake.

211 This activity could be attenuated by either centrifugation of the inhibitor stock solution or by add

212 able exception of sample volume reduction or centrifugation, one of the most widely utilized processe

213 However, centrifugation only removed a small fraction of total pa

214 k up the emulsion (that otherwise would need centrifugation or a demulsification solvent), further im

215 le cells require the use of density gradient centrifugation or antibody-based cell sorting with molec

216 icity of the protocol used, with no need for centrifugation or filtration, organic solvents or high t

217 Ribosomes were obtained by differential centrifugation or immunopurification and were digested w

218 g "off the shelf" reagents, does not require centrifugation or mechanical lysis, and can be readily i

219 This aggregation was not affected by centrifugation or pretreatment with organic solvents.

220 ds for quantifying persulfate require either centrifugation or prolonged settling times.

221 2 (P1), 1:1 dilution with 2% HNO3 and latter centrifugation (P2) or filtration (P3) and direct analys

222 Dilution with 2% HNO3 and centrifugation (P2) provided the best results, i.e. LODs

223 or filtration (P3) and direct analysis after centrifugation (P4) for the determination of total Al, B

224 readers, since studies have not always used centrifugation parameters with equal rotor sizes, angula

225 ies, including particle dispersion, settling/centrifugation, pipetting, and visible-light spectroscop

226 NARE is the elimination of dilutive wash and centrifugation processes that are fundamental to convent

227 However, in centrifugation, proteins with good affinity to the SPION

228 4 orders of magnitude lower than that using centrifugation-purification procedure, significantly sho

229 Nuclei displaced by centrifugation rapidly recentered by mechanisms that dep

230 n 25 min of operating time, density gradient centrifugation recovers an average of 33% of all sperm a

231 s, whereas nanomax was the most stable under centrifugation regardless the temperature.

232 uential washes with filtration spin cups and centrifugation remove non-specific interactions in a gen

233 Centrifugation removes large cardiomyocytes and tissue d

234 immobilized enzyme recovered by low g-force centrifugation retained 83% of its initial enzymatic act

235 Sucrose gradient centrifugation revealed chaperonin-sized complexes of bo

236 Sucrose gradient density centrifugation revealed that EaDAcT colocalizes to the s

237 r isolating mitochondria (e.g., differential centrifugation) routinely results in organelle preparati

238 ificial gravity (AG) through short-arm human centrifugation (SAHC) has been proposed as a holistic sp

239 and thus g-gradient) during short-arm human centrifugation (SAHC) upon cardiovascular responses, cer

240 After 8.5min of centrifugation, sedimented phase (surfactant-rich phase)

241 simple treatments of chemical oxidation and centrifugation separation.

242 Sucrose density gradient centrifugation showed that exposure of roots to 0.5 mM H

243 ion analysis using OptiPrep density gradient centrifugation shows an increase of functional mMOR-1 re

244 led precursor procapsids by sucrose gradient centrifugation shows that there appear to be three class

245 Differential centrifugation, size-exclusion chromatography, and prote

246 capacity to 2 mL and can reach hand-powered centrifugation speeds up to 6,000 rpm.

247 hysically stable when subjected to different centrifugation speeds.

248 blished isolation protocols require multiple centrifugation steps and substantial amounts of starting

249 organic solvents and time-consuming multiple centrifugation steps that are required in traditional RN

250 f EC cultures were subjected to differential centrifugation steps to collect larger and smaller EVs w

251 s require multiple centrifugation and vacuum-centrifugation steps, making liquid handling robot media

252 a tissue, isolated by several filtration and centrifugation steps, was subjected to alpha-synuclein/R

253 out requiring the use of organic solvents or centrifugation steps.

254 t requiring any centrifugation and/or vacuum centrifugation steps.

255 glutaraldehyde cross-linking and rate zonal centrifugation suggested that EspD in LUV membranes form

256 equipped with "two-phase" and "three-phase" centrifugation systems, respectively.

257 The combination with an on-grid centrifugation technique ensures an efficient concentrat

258 Using electron microscopy and analytical centrifugation techniques, we observed spontaneous foldi

259 Rolling circle amplification and gradient centrifugation testing were positive for encapsidated HP

260 nd that more pronounced order is obtained by centrifugation than sedimentation.

261 Owing to eliminating centrifugation, the conditions of CPE-CCT were more mode

262 We found that following sucrose gradient centrifugation, the most seed-competent fractions from P

263 This was interpreted to mean that, during centrifugation, the spherical ghosts were oriented by a

264 y, by dehydration of silica particles during centrifugation, the spontaneous formation of capillary w

265 aqueous micelle phase has been separated by centrifugation, then it was treated with a mixture of H2

266 hSOD1 aggregate seeds that were prepared by centrifugation through a density cushion were inoculated

267 ration of the chelating ligand, salt effect, centrifugation time and sample volume were investigated

268 t, sample pH, salting-out effect, vortex and centrifugation time were studied.

269 ations of TMAH and EDTA, ultrasonication and centrifugation times were all optimised for analytes usi

270 uman cells are fragile and require only mild centrifugation to be dismantled and subsequently reconst

271 udy demonstrates the utility of differential centrifugation to enrich for mitochondrion within cell e

272 l that uses simple and rapid Percoll density centrifugation to enrich RV-susceptible activated CD8(+)

273 We used density gradient centrifugation to isolate LDL in plasma pooled from 5 no

274 Here, we used gentle centrifugation to obtain a mucus supernatant showing no

275 the exfoliation of layered MFI, followed by centrifugation to remove non-exfoliated particles.

276 However, centrifugation to remove SWCNTs and adsorbed EE2 signifi

277 % NaOH at 30 degrees C for 30min followed by centrifugation to separate the starch rich and the prote

278 ribosome-bound species) with sucrose density centrifugation (to recover intact 70S monosomes).

279 Preparation of the complex involved centrifugation (to remove insoluble iron), ultrafiltrati

280 tal Exosome Isolation Kit or by differential centrifugation/ultracentrifugation.

281 This is an alternative to the use of centrifugation, ultrafiltration, and evaporative methods

282 ith all cross-linked proteins is isolated by centrifugation under denaturing conditions.

283 ssociated hESCs in the absence of ROCK-i and centrifugation was achieved within an optimal range of i

284 Rho-associated kinase inhibitor (ROCK-i), or centrifugation was needed and the side effects of ROCK-i

285 ranule fraction (G(in)) produced by egg yolk centrifugation was pressure-treated at 400 and 600 MPa f

286 Centrifugation was required in 59% cases with scanty spu

287 ubated ex vivo +/- insulin, and differential centrifugation was used to isolate cytosolic and membran

288 by dynamic light scattering and differential centrifugation, was less straightforward.

289 assessed using the Enslin-Neff and drainage centrifugation water retention capacity methods, thermog

290 fluorescence microscopy and density gradient centrifugation we found that mouse TRPA1 localizes prefe

291 olated by immunoaffinity and sucrose cushion centrifugation were characterized by morphologic, bioche

292 Settling and centrifugation were conducted in crude sunflower and pal

293 unt of Na(2)SO(4) salt (5%, w/v) and time of centrifugation were investigated and optimized by experi

294 fter fermentation by racking, filtration, or centrifugation, which add costs to the overall process a

295 ze exclusion chromatography and differential centrifugation, which produces pure and concentrated EVs

296 e purification approach utilizing rate-zonal centrifugation, which provides comparable separation res

297 ferent individuals (n=3) submitted or not to centrifugation (whole vs clarified saliva).

298 granule transcriptome based on differential centrifugation with and without subsequent stress granul

299 y TaqMan, there was poor concordance without centrifugation with the TaqMan platform with only 40% 'u

300 sedimented organic phase was obtained after centrifugation, withdrawn into the microsyringe and dire