ライフサイエンスコーパス: centriole

1 to a specific site to generate a single new centriole.

2 incorporation of both STIL and CPAP into the centriole.

3 ciliogenesis-initiating events at the mother centriole.

4 ed pericentriolar material around the mother centriole.

5 BXW7 strongly associated with HsSAS-6 at the centriole.

6 le wall along the central core region of the centriole.

7 ar material (PCM) to the proximal end of the centriole.

8 n an older "mother" and a younger "daughter" centriole.

9 factors, importantly including supernumerary centrioles.

10 ease gene through an ill-defined function at centrioles.

11 r pathway in association with the two parent centrioles.

12 on the surface and in the vicinity of parent centrioles.

13 iogenesis in cells with manipulated daughter centrioles.

14 at multiple sites, leading to supernumerary centrioles.

15 in over-elongated and structurally aberrant centrioles.

16 estraining HsSAS-6 recruitment to the mother centriole, a molecular mechanism that controls supernume

17 ere, we show that rcd4 mutants exhibit fewer centrioles, aberrant mitoses, and reduced basal bodies i

18 Quantification of centriole abundance in vitro and in vivo identified a li

19 of PC caused an increase in deuterosome and centriole abundance, highlighting the presence of a comp

20 To test whether cells progressively lost centrioles after becoming near-tetraploid, we transientl

21 Additionally, centrioles align between the nucleus and the leading edg

22 and E2F4 mutant proteins we demonstrate that centriole amplification is crucially dependent on these

23 These results support a model in which centriole amplification occurs during a transient state

24 previously unsuspected cytoplasmic events of centriole amplification, providing new perspectives for

25 analysed the number of centrioles revealing centriole amplification.

26 C does not perturb deuterosome formation and centriole amplification.

27 tween 100-600 centrioles by a process termed centriole amplification.

28 Most centrioles amplified by MCCs grow on the surface of orga

29 genesis, indicating tissue-specific roles in centriole and basal body formation.

30 ic surface area-dependent mechanism controls centriole and cilia abundance in multiciliated cells.

31 hnologies have expanded our understanding of centriole and cilium biology, revealing exciting avenues

32 uitment of the ciliary vesicle to the mother centriole and its subsequent maturation, docking, and fu

33 that BCCIP localizes proximal to the mother centriole and participates in microtubule organization a

34 lays important roles in the orchestration of centriole and PCM assembly.

35 We tested multiple anti-centriole and pericentriolar-material antibodies to iden

36 hat FBXW7 is broadly localized on the mother centriole and that its presence is reduced at the site w

37 of two microtubule-derived structures called centrioles and an amorphous mass of pericentriolar mater

38 ts in our understanding of the biogenesis of centrioles and cilia and the regulatory controls that go

39 Yet, how MCC regulate the precise number of centrioles and cilia remains unknown.

40 Remarkably, MCCs lacking parent centrioles and deuterosomes also amplify the appropriate

41 Here, we address in vivo the role of centrioles and the accumulation of MTs on the protrusive

42 tion of distal appendage markers on daughter centrioles and the loss of procentriolar markers.

43 Oocytes of many species lack centrioles and therefore form acentriolar spindles.

44 It localizes around the centrioles and to actin cytoskeleton.

45 explain the origin of structurally aberrant centrioles and why centriole numerical and structural de

46 migration, that it localizes to the maternal centriole, and that it forms a complex with many other p

47 lticiliated cells (MCCs), which contain ~150 centrioles, and ionocytes (ICs), which contain two centr

48 ulticiliated cells, originates from parental centrioles, and its cellular position is biased and depe

49 In the absence of F-actin, the sperm DNA, centrioles, and organelles were transported as a unit wi

50 e, where the majority of cenexin, the mother centriole appendage protein and PLK1 binding partner, re

51 tability of microtubule triplets, as well as centriole architectural integrity remain poorly understo

52 conserved molecular player participating in centriole architecture integrity.

53 Distal appendages (DAs) of the mother centriole are essential for the initial steps of cilioge

54 Additionally, we found that centrioles are amplified in immediate neuronal precursor

55 We show that centrioles are amplified via centriole rosette formation

56 Centrioles are characterized by a nine-fold arrangement

57 Centrioles are composed of long-lived microtubules arran

58 Centrioles are conserved organelles fundamental for the

59 Centrioles are core structural elements of both centroso

60 These aberrant centrioles are formed de novo each cell cycle, but are u

61 Centrioles are key eukaryotic organelles that are respon

62 Although centrioles are known to regulate PCM assembly, it is les

63 Centrioles are microtubule (MT)-based structures that pr

64 Centrioles are precisely built microtubule-based structu

65 In other species, oocyte-derived centrioles are removed around the time of fertilization

66 Centrioles are small barrel-shaped structures that form

67 Centrioles are vital cellular structures that form centr

68 , little in our extant knowledge conveys how centrioles arise.

69 ntrates microtubule-nucleators, but also the centriole assembly machinery, promoting biogenesis close

70 rotein 6 (SAS-6) is an essential step in the centriole assembly process and may act as trigger for th

71 CEP135, a centriole assembly protein, is dysregulated in some brea

72 Centriole assembly requires oligomerization of the essen

73 s interaction is conserved and important for centriole assembly, particularly its elongation.

74 known whether and how the PCM contributes to centriole assembly.

75 oligomerization in vitro and for function in centriole assembly.

76 glutamylase residues 330-624 and spindle and centriole associated protein 1, residues 549-855 (SPICE1

77 iments, we propose that the establishment of centriole asymmetry in mitosis primes biased interphase

78 tes usually have multiple cilia, each with a centriole at its base.

79 and increases its recruitment to the mother centriole at multiple sites, leading to supernumerary ce

80 using this model, we assessed the number of centrioles at different time-points after tetraploidizat

81 Two evolutionarily conserved centriole/basal body cartwheel proteins, TbSAS-6 and TbB

82 ing molecularly distinct mother and daughter centrioles before interphase.

83 Centriole biogenesis and homeostasis is tightly regulate

84 an overview of our current understanding of centriole biogenesis and how variations around the same

85 HsSAS-6 for degradation and thereby controls centriole biogenesis by restraining HsSAS-6 recruitment

86 Centriole biogenesis is regulated by Polo-like kinase 4

87 of the growing centriole initiates and times centriole biogenesis to ensure that centrioles grow at t

88 It is critical in cell division, centriole biogenesis, and neuronal morphogenesis.

89 o-like kinase 4 (Plk4) is a key regulator of centriole biogenesis.

90 ) are capable of selecting a single site for centriole biogenesis.

91 ondensate to serve as an assembling body for centriole biogenesis.

92 assembly around centriolar MTs and promoting centriole biogenesis.

93 docking of the ciliary vesicle to the mother centriole but prior to axoneme elongation and fusion of

94 of Aurora kinase activity, SPICE1 remains at centrioles but does not target to the spindle.

95 old is initially assembled around the mother centriole, but it cannot expand outwards, and centrosome

96 ue homeostasis, are extensions of the mother centriole, but the mechanisms that remodel the centriole

97 as been shown that TTBK2 is recruited to the centriole by distal appendage protein CEP164, little is

98 ese cilia, each MCC produces between 100-600 centrioles by a process termed centriole amplification.

99 de up of rootletin filaments anchored to the centrioles by C-NAP1.

100 Centrioles can also arise de novo, and thus the longstan

101 Centrioles can also be modified to form basal bodies, wh

102 utonomously of it-potentially explaining why centrioles can duplicate independently of cell-cycle pro

103 Cells with amplified centrioles can go on to divide, with centrioles clustere

104 Together, these findings indicate that centrioles can persist when Plk1 activity is impaired, a

105 Using Drosophila, we identify a centriole-centric mechanism that ensures proper proximal

106 ecular mechanism that controls supernumerary centrioles/centrosomes and the maintenance of bipolar sp

107 plified centrioles can go on to divide, with centrioles clustered at each pole.

108 Rcd4 is a poorly characterized Drosophila centriole component whose mammalian counterpart, PPP1R35

109 Here we investigate the interaction between centriole components and the PCM by taking advantage of

110 resolution microscopy, and identification of centriole components have accelerated our understanding

111 ed that several ectopically-expressed animal centriole components such as SAS-6 are recruited to the

112 cant progress has been made in understanding centriole composition, we have limited knowledge of how

113 Centrioles constitute the core of centrosomes, which org

114 cells either inherit the mother or daughter-centriole-containing centrosome, providing a possible me

115 s of positive selection for cells with extra centrioles, continuous generation of such centrioles, or

116 ocyte to ensure that only the two functional centrioles contributed by the sperm are present in the z

117 Centriole copy number is tightly maintained by the once-

118 tion, phenotypes that are strongly linked to centriole defects.

119 oscopy and electron tomography, we find that centrioles degenerate early during ciliogenesis.

120 Centriole deletion altered microtubule architecture as e

121 he primary cilium is nucleated by the mother centriole-derived basal body (BB) via as yet poorly char

122 support a model in which ANKRD26 initiates a centriole-derived signal to limit cell proliferation in

123 erile, reflecting a requirement for Ana3 for centriole development in the male germ line, rcd4 mutant

124 Nevertheless, CTLs lacking centrioles did display substantially reduced killing pot

125 oles, and ionocytes (ICs), which contain two centrioles, differentiate during the same developmental

126 Loss of Dbf causes premature centriole disengagement and affects spindle structure, c

127 Centrosome fragmentation and precocious centriole disengagement depend on separase and anaphase-

128 he initiation of centriole licensing through centriole disengagement, at which point the ability to m

129 However, cells with extra centrioles display heterogenous phenotypes including ext

130 Using a genome-wide screen, we identify centriole distal appendages as critical for PIDDosome ac

131 and recruit the PIDDosome component PIDD1 to centriole distal appendages, and this interaction is req

132 We discover that centrioles do not have an elongation monitoring mechanis

133 que opportunity to address this question, as centrioles do not persist at the base of mature cilia.

134 This results in erroneous, lateral centriole docking to the nucleus, leading to spermatid d

135 We further show that Nup188 functions in centriole duplication at or upstream of Sas6 loading.

136 n development, and human mutations affecting centriole duplication cause microcephaly.

137 The centriole duplication cycle normally ensures that centri

138 WBP11 depletion causes centriole duplication defects, in part by causing a rapi

139 pindle pole body, as a critical regulator of centriole duplication in mammalian cells.

140 ional splicing factors that are required for centriole duplication interact with WBP11 and are requir

141 Tight control of centriole duplication is critical for normal chromosome

142 onsequences of deregulating Plk4 (the master centriole duplication kinase) activity in Drosophila asy

143 Centriole duplication occurs once in each cell cycle to

144 Centriole duplication occurs once per cell cycle and is

145 t the USP9X protection of STIL to facilitate centriole duplication underlies roles of both proteins i

146 that SAS-7 functions at the earliest step in centriole duplication yet identified and plays important

147 During centriole duplication, a preprocentriole forms at a sing

148 r protein kinase and the master regulator of centriole duplication, but it may play additional roles

149 licing factors leads to a specific defect in centriole duplication, but the cause of this deficit rem

150 e for CEP131 localization, ciliogenesis, and centriole duplication, it is essential for maintaining t

151 racts with Sak/Plk4, a critical regulator of centriole duplication, more strongly at the GSC mother c

152 of centrosomes due to a failure in daughter centriole duplication, suggesting that Alms1a has a stem

153 rupting locus gene (Stil), key regulators of centriole duplication.

154 et of splicing factors leads to a failure of centriole duplication.

155 le cartwheel assembly, and thereby regulates centriole duplication.

156 ctor, WBP11, as a novel protein required for centriole duplication.

157 the centrosome to stabilize STIL and promote centriole duplication.

158 ponents, including Plk4, a key regulator for centriole duplication.

159 form of CEP135, CEP135(mini), that represses centriole duplication.

160 deubiquitylate STIL, a critical regulator of centriole duplication.

161 ing the self-assembly abrogate Plk4-mediated centriole duplication.

162 erly recruitment of Plk4, a key regulator of centriole duplication.

163 tethering preciliary vesicles to the mother centriole during ciliogenesis.

164 e Centrobin (Cnb) accumulate on the daughter centriole during mitosis, thereby generating molecularly

165 structures called deuterosomes that nucleate centrioles during amplification.

166 say the role of Polo-like kinase 1 (Plk1) in centriole elimination.

167 erpart, PPP1R35, is suggested to function in centriole elongation and conversion to centrosomes.

168 1, which we uncover as a novel regulator of centriole elongation in human cycling cells.

169 s mRNA and protein to the earliest stages of centriole elongation.

170 ted phosphorylation, whereas Polo's daughter centriole enrichment requires both Wdr62 and Cnb.

171 Centrioles form the core of centrosomes, which organize

172 ext cell cycle, leading to a futile cycle of centriole formation and disintegration.

173 und the same theme generate alternatives for centriole formation and function.

174 the first recruited to the site of daughter centriole formation and regulates the centriolar localiz

175 Ana2 at the procentriole to induce daughter centriole formation.

176 how PLK4 activity controls specific steps in centriole formation.

177 h flexible SAS-6 segments for the control of centriole formation.

178 king advantage of fission yeast, which has a centriole-free, PCM-containing centrosome, the SPB.

179 Formation of a single new centriole from a pre-existing centriole is strictly cont

180 ase impairment with BI-2536 does not protect centrioles from removal in the bat star Patiria miniata.

181 nd times centriole biogenesis to ensure that centrioles grow at the right time and to the right size.

182 k4) and initiates in S-phase when a daughter centriole grows from the side of a pre-existing mother.

183 complex is concentrically arranged around a centriole in a Cep57-in and Cep152-out manner.

184 oteins are concentrically localized around a centriole in a highly organized manner.

185 url-4 transiently associated with the mother centriole in a process that required mother-daughter cen

186 oma brucei is structurally equivalent to the centriole in animals and functions in the nucleation of

187 The mother centriole in RGPs develops distal appendages that anchor

188 es at the subdistal appendages of the mother centriole in specific subtypes of neural stem cells, and

189 Although about half of cells with extra centrioles in a population were able to divide, the exte

190 e, and rosette-bearing cells often have free centrioles in addition.

191 Here, we report that centrioles in delta-tubulin and epsilon-tubulin null mut

192 bryonic utricles by live-imaging GFP-labeled centrioles in HCs.

193 r by grossly asymmetric segregation of extra centrioles in mitosis.

194 Plk4 oscillation at the base of the growing centriole initiates and times centriole biogenesis to en

195 somes also amplify the appropriate number of centrioles inside a cloud of pericentriolar and fibrogra

196 annot be explained by limited duplication of centrioles, instability of extra centrioles, or by gross

197 The daughter centriole invariably migrated ahead of the MC from the c

198 The centriole is a multifunctional structure that organizes

199 The centriole is an ancient microtubule-based organelle with

200 Centriole is an essential structure with multiple functi

201 f a single new centriole from a pre-existing centriole is strictly controlled to maintain correct cen

202 ioning of PLP to more distal portions of the centriole is sufficient to redistribute PCM and microtub

203 how the epithelium generates cells with many centrioles is not yet understood.

204 le cilia assembly is absolutely dependent on centrioles, it is not known to what extent they contribu

205 lthough Plk1 localizes around oocyte-derived centrioles, kinase impairment with BI-2536 does not prot

206 onstrating that cell division with amplified centrioles, known to be tolerated in disease states, can

207 Plk1 levels lead to the formation of shorter centrioles lacking a full set of microtubule triplets, i

208 leotide exchange factor (GEF), to the mother centriole, leading to Rab8 activation and cilium growth.

209 ribute PCM and microtubules along the entire centriole length.

210 itotic progression trigger the initiation of centriole licensing through centriole disengagement, at

211 ver, we uncover that all four oocyte-derived centrioles lose microtubule nucleating activity when ret

212 how yeasts kept this interaction even after centriole loss and showed that the conserved calmodulin-

213 ack triplet microtubules and fail to undergo centriole maturation.

214 The mother centriole (MC) forms the base of the kinocilium and the

215 Although the mother centriole mediates most centrosome-dependent processes,

216 mmalian distal appendages associate with two centriole microtubule triplets via an elaborate filament

217 pendage proteins localize in relation to the centriole microtubules and appendage electron densities.

218 gesting that Emx2 pre-patterned HCs prior to centriole migration.

219 he centrosome, leading to PCM dispersion and centriole mis-positioning at the spindle poles.

220 Centrioles must be eliminated or inactivated from the oo

221 Centrioles must duplicate as cells progress through the

222 Interestingly, two such centrioles nevertheless retain the centriolar markers mE

223 ming of insertion is modulated by changes in centriole number and the accumulation of acetylated MTs.

224 anisms responsible for this return to normal centriole number at the population level in human retina

225 kinase PLK4, human cells return to a normal centriole number during the proliferation of the populat

226 Modulating centriole number in 3D cell cultures has been shown to i

227 We find that the return to normal centriole number in the population of induced cells cann

228 iole duplication cycle normally ensures that centriole number is maintained at two centrioles per G1

229 These data show that the centriole number is set independently of their nucleatio

230 y manipulating cell size, we discovered that centriole number scales with surface area.

231 Following an artificial increase in centriole number without tetraploidization due to transi

232 g SPICE1 phosphorylation results in abnormal centriole number, spindle multipolarity, and chromosome

233 tances can result in an aberrant increase in centriole number-a phenotype that is particularly preval

234 ocephalin caused the most robust increase in centriole number.

235 to FBXW7-mediated degradation and control of centriole number.

236 linear relationship between surface area and centriole number.

237 nt for the observed rate of return to normal centriole number.

238 of structurally aberrant centrioles and why centriole numerical and structural defects coexist in tu

239 mutants are fertile and have male germ line centrioles of normal length.

240 is achieved through a massive production of centrioles on the surface and in the vicinity of parent

241 The centriole, or basal body, is the center of attachment be

242 ra centrioles, continuous generation of such centrioles, or alleviation of the disadvantageous growth

243 lication of centrioles, instability of extra centrioles, or by grossly asymmetric segregation of extr

244 Centrioles organize the microtubule network and mitotic

245 A structural motif called the G-box in the centriole outer wall protein Sas4 interacts with a short

246 We identify that mitotic centriole over-elongation is dependent on mitotic Polo-l

247 at dysregulation of CEP135 isoforms promotes centriole overduplication and contributes to chromosome

248 We examined the contribution of centriole overduplication to CA and the consequences for

249 t of genes, which cause cilia shortening and centriole overduplication.

250 In mammals, centrioles participate in brain development, and human m

251 Airway progenitor cells contain two parental centrioles (PC) and form structures called deuterosomes

252 s that centriole number is maintained at two centrioles per G1 cell.

253 Centrioles play a key role in the development of the fly

254 Centrioles play critical roles in organizing the assembl

255 ploidization, a small fraction retains extra centrioles, potentially resulting in CIN.

256 ucleation platforms and suggest that massive centriole production in MCCs is a robust process that ca

257 Among five known core centriole proteins, SPD-2/Cep192 is the first recruited

258 e in a process that required mother-daughter centriole proximity.

259 Centrosomes are formed when mother centrioles recruit pericentriolar material (PCM) around

260 Rsg1 mother centrioles recruit proteins required for cilia initiatio

261 through Spd2 phosphorylation, which induces centriole release from the apical cortex.

262 loss of microtubule nucleating activity and centriole removal.

263 urther diversity in the mechanisms governing centriole removal.

264 de novo, and thus the longstanding focus on centriole 'replication' may have led us astray from grou

265 as occurring, we next analysed the number of centrioles revealing centriole amplification.

266 We show that centrioles are amplified via centriole rosette formation in both embryonic developmen

267 on and function of cilia depends on a set of centriole's distal appendages.

268 and had an increased frequency of premature centriole separation, accompanied by reduced density of

269 We conclude that centriole splitting reduces the local density of key cen

270 expansion microscopy to study the origins of centriole structural aberrations in large populations of

271 ion of inner scaffold components, leading to centriole structural abnormalities in human cells.

272 Aberrations in centriole structure are common in tumors, yet how these

273 Analysis of centriole structure is difficult because it requires dem

274 Here, we show that PLK4 phosphorylates its centriole substrate STIL on a conserved site, S428, to p

275 The centrosome, consisting of two centrioles surrounded by a dense network of proteins, is

276 The centrosome is composed of two centrioles surrounded by a microtubule-nucleating perice

277 The centrosome, composed of two centrioles surrounded by pericentriolar material, is the

278 e the centrosomes, which are composed of two centrioles, surrounded by a protein-rich matrix of elect

279 tions upstream of MSO control, orchestrating centriole symmetry breaking and consequently centrosome

280 While the distal end of the centriole templates the cilia, the proximal end associat

281 thought to form a scaffold around the mother centriole that recruits other components of the mitotic

282 w no evidence of cytoplasmic buds containing centrioles that are produced from the first abortive mei

283 liated cells (MCCs) amplify large numbers of centrioles that convert into basal bodies, which are req

284 we review the structure and functions of the centriole, the centrosome, and the basal body in differe

285 One spindle pole contains the oldest mitotic centriole, the mother centriole, where the majority of c

286 el, we employed a genetic strategy to delete centrioles, the core structural components of the centro

287 ntriole forms at a single site on the mother centriole through a process that includes the hierarchic

288 ciliary vesicle (CV) stage to promote mother centriole to basal body transition.

289 We showed that the use of the centriole to form primary cilia blocks centrosome format

290 mary cilium must be disassembled to free its centriole to form the centrosome, a necessary structure

291 ntriole, but the mechanisms that remodel the centriole to promote cilia initiation are poorly underst

292 rotein called SAS-7 is required for daughter centrioles to become mothers in C. elegans.

293 ipates directly in the mitotic conversion of centrioles to centrosomes, but both are required to load

294 rkers of microtubule nucleating activity and centrioles to investigate this question.

295 Comparing HCs between utricular regions, centriole trajectories were similar but they migrated to

296 hin hours during a critical time-window when centriole trajectory was responsive to Emx2.

297 Ectopic Emx2, however, reversed centriole trajectory within hours during a critical time

298 linking the cartwheel to microtubules of the centriole wall.

299 ins the oldest mitotic centriole, the mother centriole, where the majority of cenexin, the mother cen

300 s a component of PCM needed to duplicate the centriole with implications for congenital heart disease

301 g deuterosomes amplify the correct number of centrioles with normal step-wise kinetics.