ライフサイエンスコーパス: centrosome

1 s of the plus ends of growing MTs and at the centrosome.

2 selectively inherits the mother or daughter centrosome.

3 ioles, the core structural components of the centrosome.

4 y in the zygote, be poised to respond to the centrosome.

5 ultimately inactivating MTOC function at the centrosome.

6 egulation of the EB1-p150 association at the centrosome.

7 vicinal to the inner core of the interphase centrosome.

8 or form new microtubules off the perinuclear centrosome.

9 for PIDDosome activation in cells with extra centrosomes.

10 r successful cell division in the absence of centrosomes.

11 and PIDDosome activation in cells with extra centrosomes.

12 activity to ensure low DA levels at mitotic centrosomes.

13 at least in part, by mobilizing ARP2 to the centrosomes.

14 endencies of satellite composition on intact centrosomes.

15 uire whole cell volumes in which to scan for centrosomes.

16 of soluble and polymeric tubulin at mitotic centrosomes.

17 ating distinct states of mother and daughter centrosomes.

18 responsible for the positioning of multiple centrosomes.

19 scence, we confirm that PLK4 is localized to centrosomes.

20 n of chromosomes and the appearance of multi-centrosomes.

21 e, we set out to prevent clustering of extra centrosomes.

22 in response to DNA damage and supernumerary centrosomes.

23 ibit a significant increase in the number of centrosomes.

24 on in centriole elongation and conversion to centrosomes.

25 sperm-derived nuclei and their accompanying centrosomes.

26 ved in culture have been shown to lack extra centrosomes.

27 pindles contain asymmetrically sized mitotic centrosomes (2.14 +/- 0.13-fold difference between the t

28 ationally screen effective forces between 1) centrosomes, 2) centrosomes and kinetochores, 3) centros

29 We find that the mitotic centrosome, a structure that assembles the mitotic spind

30 Loss of such coupling produces supernumerary centrosomes, a condition called centrosome amplification

31 Centrosome aberrations are commonly observed in human tu

32 However, centrosome aberrations can also disrupt tissue architect

33 In contrast, most cancer cells have centrosome aberrations, including supernumerary centroso

34 aploids maintained in culture do not present centrosome aberrations.

35 Centrosome abnormalities are emerging hallmarks of cance

36 ook2 regulates microtubule nucleation at the centrosome; accordingly, Hook2-depleted cells have reduc

37 polarizing cues, how is specificity for the centrosome achieved?

38 to inhibit cell proliferation, but how extra centrosomes activate the PIDDosome remains unclear.

39 The centrosome acts as a microtubule organizing center (MTOC

40 ry coarse-grained Shannon information to the centrosome adjacent to the nucleus with minimum loss of

41 raction severely inhibited nuclear movement, centrosome advance proceeded unimpeded, supporting an in

42 ded, supporting an independent mechanism for centrosome advance.

43 hat although most cells reduce supernumerary centrosomes after tetraploidization, a small fraction re

44 oduced from phage DNA, which did not possess centrosomes, also acted as trigger wave sources, but pur

45 A numerical increase in centrosomes, or centrosome amplification (CA), is common in cancer and c

46 upernumerary centrosomes, a condition called centrosome amplification (CA).

47 d consistent and severe mitotic failure with centrosome amplification and multipolar spindle formatio

48 and induces mitotic abnormalities, including centrosome amplification and multipolar spindle formatio

49 Centrosome amplification is frequently observed in tumor

50 n breast cancer; however, the role of p15 in centrosome amplification is unknown.

51 In non-transformed cells, centrosome amplification triggers PIDDosome activation a

52 required for PIDDosome activation following centrosome amplification.

53 l to limit cell proliferation in response to centrosome amplification.

54 nuclear envelope (NE), which is required for centrosome anchoring to the NE.

55 was caused by asymmetric distribution of the centrosome and aberrant spindle assembly.

56 that LUZP1 associates with factors linked to centrosome and actin filaments.

57 LIN, increases the levels of MyosinVa at the centrosome and primary cilia formation.

58 Arl4D promotes the recruitment of EB1 to the centrosome and regulates MT nucleation.

59 Moreover, the centrosome and the pericentriolar material form condensa

60 , 3) centrosomes and chromosome arms, and 4) centrosomes and cell cortex to understand mechanics that

61 rosomes, 2) centrosomes and kinetochores, 3) centrosomes and chromosome arms, and 4) centrosomes and

62 is, where it is dynamically recruited to the centrosomes and chromosomes during mitosis.

63 eless granules that localize and move around centrosomes and cilia.

64 teomes, and disease links of satellites with centrosomes and cilia.

65 t microtubule-based structures that assemble centrosomes and cilia.

66 tional diversity and their functions outside centrosomes and cilia.

67 oles are vital cellular structures that form centrosomes and cilia.

68 ent to alter cognate protein localization to centrosomes and impair spindle morphogenesis and genome

69 effective forces between 1) centrosomes, 2) centrosomes and kinetochores, 3) centrosomes and chromos

70 In diverse cell types, mRNAs localize to centrosomes and may contribute to changes in PCM abundan

71 GSK3beta binds to centrosomes and microtubules, and lymphoma cells treated

72 es length-dependent pulling forces that move centrosomes and MTs outwards, while other components of

73 We show that during prophase, centrosomes and nucleus reorient so that centrosomes are

74 Centrosomes and spindle pole bodies (SPBs) are membranel

75 y directing the localization of Cdc13-CDK to centrosomes and that this localization of CDK contribute

76 that Arl4D colocalized with gamma-tubulin in centrosomes and the depletion of Arl4D resulted in a cen

77 anism that controls supernumerary centrioles/centrosomes and the maintenance of bipolar spindles.

78 Cilia arise from centrosomes and their formation and function is governed

79 ion by regulating the structure of host cell centrosomes and thereby nuclear lamina integrity.

80 TBCD to support microtubule nucleation from centrosomes and with ARF6 in cytokinesis.

81 ous components are localized in the nucleus, centrosome, and cytoplasm.

82 ts in mitosis and cytokinesis, supernumerary centrosomes, and compromised cell-cycle checkpoints, all

83 ons, but it seems premature to conclude that centrosomes, and perhaps other membraneless organelles,

84 trosome aberrations, including supernumerary centrosomes, and this correlates with aneuploidy and gen

85 polyploidy, increased numbers of duplicated centrosomes, and vulnerability to anaphase or mitotic ca

86 distinctive subcellular organization of the centrosome are largely unknown.

87 Even if centromeres and centrosomes are connected via MTs in mitosis, it is not

88 During mitotic exit, however, centrosomes are deformed and fractured by those same for

89 Centrosomes are formed when mother centrioles recruit pe

90 We have previously shown that centrosomes are key players in the initiation of subcell

91 Centrosomes are microtubule-organizing centers required

92 How centrosomes are positioned in space and time through the

93 se, centrosomes and nucleus reorient so that centrosomes are positioned on the shortest nuclear axis

94 Across most sexually reproducing animals, centrosomes are provided to the oocyte through fertiliza

95 Moreover, we find that centrosome area in near-tetraploids is twice as large as

96 elegans and zebrafish [2, 3], where mitotic centrosome area scales more closely with changes in cell

97 is leads to the accumulation of actin around centrosomes as cells enter anaphase and to a correspondi

98 This model has anticipated analogous centrosome asymmetries featured in self-renewing stem ce

99 However, the mechanisms regulating centrosome asymmetry and biased centrosome segregation a

100 r and outer sphere that are removed from the centrosome at different rates and using different behavi

101 We show that a C. elegans one-cell stage centrosome at metaphase contains >10,000 microtubules wi

102 Here, we established a method of quantifying centrosomes at a single-cell level in different types of

103 Because astral MT minus-ends are anchored by centrosomes at spindle poles, we hypothesized that the M

104 a mitotic spindle, a molecular machine with centrosomes at two opposing cell poles and chromosomes a

105 a multicentrosomal cell: 1) the strengths of centrosomes' attraction to each other and to the cell co

106 tional to each other and 2) the strengths of centrosomes' attraction to kinetochores and repulsion fr

107 P1 localizes to both actin filaments and the centrosome/basal body.

108 Although the asymmetric centrosome behavior is widely conserved, its biological

109 ating centrosome function but also links the centrosome biogenesis machinery with the MSO apparatus.

110 Centrosomes break symmetry in the C. elegans one-cell em

111 y in the mitotic conversion of centrioles to centrosomes, but both are required to load Ana1, which i

112 uncated isoforms localize to neuroprogenitor centrosomes, but full-length OCLN transiently localizes

113 osomes with a microtubule spindle that lacks centrosomes, but the mechanisms by which acentrosomal sp

114 The unpaired chromosome remains tethered to centrosomes by lengthening kinetochore microtubules, whi

115 of the old spindle pole body (SPB, the yeast centrosome) by the bud.

116 Centrosomes catalyse the formation of microtubules neede

117 How the functional material properties of centrosomes change throughout the cell cycle, and how th

118 d as the "third component" of the vertebrate centrosome/cilium complex, which profoundly changes the

119 n suppressed multipolarity by improving both centrosome clustering and pole coalescence.

120 me during a bipolar division with asymmetric centrosome clustering are favored for long-term survival

121 We show that even if an initial centrosome clustering step can occur at mitotic entry, t

122 To evaluate whether centrosome clustering was occurring, we next analysed th

123 roteins, which showed striking enrichment in centrosome components.

124 Centrosomes concentrate soluble alpha/beta tubulin by ab

125 Finally, we observe this centrosome configuration favors the establishment of an

126 so that it coincides with maturation of the centrosome cue.

127 This causes cells to undergo centrosome de-clustering, prolonged multipolar mitosis,

128 d brain size without correcting the upstream centrosome defects or extended mitosis.

129 Here, we discuss how centrosome defects promote invasive behaviors that may c

130 Here we show that centrosome depletion induces synthetic lethality in canc

131 nase 4 (PLK4) using small molecules leads to centrosome depletion, which triggers mitotic catastrophe

132 compromising nuclear lamina integrity led to centrosome detachment from the nuclear envelope and migr

133 that yeast spindle pole bodies (SPBs, yeast centrosomes) differentially control the plus-end dynamic

134 in which uniquely large zebrafish embryonic centrosomes direct spindle placement within disproportio

135 k, brittle state that enables force-mediated centrosome disassembly.

136 by those same forces, which is a key step in centrosome disassembly.

137 CDK5 localization and activation, leading to centrosome disorganization and disrupted microtubule cyt

138 tiating germ cells, results in rapid loss of centrosomes due to a failure in daughter centriole dupli

139 Centrosomes duplicate only once in coordination with the

140 ike kinase 4 (PLK4) plays a critical role in centrosome duplication for cell division.

141 loss of p15 and p16 provides conditions for centrosome duplication to become deregulated with conseq

142 ly inhibited, cell division proceeds without centrosome duplication, generating centrosome-less cells

143 Alms1a has a stem-cell-specific function in centrosome duplication.

144 ike kinase 4 (PLK4), the master regulator of centrosome duplication.

145 which tetraploid cells that inherit a single centrosome during a bipolar division with asymmetric cen

146 SFI1 localizes USP9X to the centrosome during S phase to deubiquitylate STIL, a crit

147 and outer cores was found in only one of the centrosomes during cell division, indicating distinct st

148 nein is required for ER concentration around centrosomes during late interphase.

149 nt, indicating that cen mRNA localization to centrosomes ensures mitotic fidelity.

150 olding the MEN onto spindle pole bodies (SPB-centrosome equivalent) allows the MEN to couple the fina

151 hydrophobic patch targets Cdc13 to the yeast centrosome equivalent, the spindle pole body (SPB), and

152 However, how supernumerary centrosomes evolve during the emergence of tetraploid ce

153 During mitosis, the centrosome expands its capacity to nucleate microtubules

154 ata2 recruits the deubiquitinase CYLD to the centrosome for deubiquitination of polo-like kinase 4 (P

155 s demonstrate that centriolar satellites and centrosomes form independently but share a substantial f

156 f the centriole to form primary cilia blocks centrosome formation and mitotic spindle assembly and pr

157 t only reveals a role for Plk4 in regulating centrosome function but also links the centrosome biogen

158 duplication, more strongly at the GSC mother centrosome, further supporting Alms1a's unique role in G

159 oid chromosome number while losing the extra centrosomes gained at the time of tetraploidization.

160 Thus, we propose that mutations in centrosome genes cause microcephaly by delaying mitosis

161 Mutations in centrosome genes deplete neural progenitor cells (NPCs)

162 Mutations in centrosome genes reduce MSO fidelity, leading to tissue

163 Besides the centrosome, Hook2 localizes to and recruits dynactin and

164 work in interphase cells has implicated the centrosome in both microtubule and actin nucleation, sug

165 lts reveal an unexpected role for the intact centrosome in controlling the capacity but not the speci

166 Alstrom syndrome, is enriched on the mother centrosome in Drosophila male germline stem cells (GSCs)

167 ts indicate a previously unknown role of the centrosome in regulating the mechanical features of neur

168 ng function tailored to the unique bipartite centrosome in the Apicomplexa.

169 olar mature neurons rotatin localizes at the centrosome in the leading edge.

170 rucial for the proper cell division, and two centrosomes in animal cells naturally become two spindle

171 of dimeric and polymeric tubulin at mitotic centrosomes in early C. elegans embryos.

172 bic patch prevents cyclin B1 localization to centrosomes in human cells, suggesting that this mechani

173 eveloped a single-cell assay for quantifying centrosomes in human prostate tissue.

174 Microtubules assembled from artificial centrosomes in microfluidic chambers of defined size are

175 DAs are released from centrosomes in mitosis by an undefined mechanism.

176 acted as trigger wave sources, but purified centrosomes in the absence of nuclei did not.

177 igate the regulation of mRNA localization to centrosomes in the rapidly cycling Drosophila melanogast

178 In 17q23-amplified cells that lack centrosomes, increased levels of TRIM37 block the format

179 IM37 prevents PLK4 from self-assembling into centrosome-independent condensates that serve as ectopic

180 re recent work uncovered several additional, centrosome-independent microtubule generation pathways,

181 In the absence of TgCep250, stray centrosome inner and outer core foci were observed.

182 roliferation of keratinocytes by maintaining centrosome integrity during mitosis through interacting

183 hat previously would not overduplicate their centrosomes into cells that did.

184 e to assemble bipolar spindles by clustering centrosomes into two spindle poles.

185 The centrosome is composed of two centrioles surrounded by a

186 Polo-dependent phosphorylation of Cnn at the centrosome is crucial for scaffold assembly.

187 In RGPs, the centrosome is positioned away from the nucleus at the ap

188 The centrosome is the microtubule organizing center of human

189 The microtubule-nucleating activity of centrosomes is conferred by the pericentriolar material

190 ng that microtubule nucleation in C. elegans centrosomes is driven in part by concentrating soluble t

191 We find that RNA localization to centrosomes is regulated during the cell cycle and devel

192 We postulate that the timely separation of centrosomes is regulated in a cell type-dependent manner

193 unctional importance of mRNA localization to centrosomes is unclear.

194 dle formation in yeast requires insertion of centrosomes (known as spindle pole bodies [SPBs]) into f

195 s includes release of MT minus-ends from the centrosome, leading to PCM dispersion and centriole mis-

196 sophila, where departure of Polo kinase from centrosomes leads to loss of microtubule nucleating acti

197 s without centrosome duplication, generating centrosome-less cells that exhibit delayed, acentrosomal

198 have a role in cellular processes including centrosome localization, cell proliferation, and tumorig

199 A centrosome-localized calcium signal is essential for mam

200 Phosphorylated RAB10 is recruited to centrosome-localized RILPL1, which may interfere with ci

201 In anaphase, centrosomes lose PLK-1 and SPD-2 and transition to a wea

202 Our findings suggest that centrosome loss could create a cellular crisis with onco

203 By chemically or genetically inducing centrosome loss in nontumorigenic prostate epithelial ce

204 ultured cells, mitotic delays resulting from centrosome loss prevent the growth of unfit daughter cel

205 Strikingly, transient or chronic centrosome loss transformed prostate epithelial cells, w

206 Surprisingly, centrosome loss-which has not been described in human ca

207 cifically about the mechanisms(s) underlying centrosome loss.

208 RIM37 causes genomic instability by delaying centrosome maturation and separation at mitotic entry, a

209 f human smooth muscle cells, which regulated centrosome maturation and spindle assembly.

210 entriole, but it cannot expand outwards, and centrosome maturation fails.

211 s prepare to enter mitosis (a process termed centrosome maturation), but it is unclear how this expan

212 inhibited paxillin Ser-272 phosphorylation, centrosome maturation, and cell division.

213 Centrosome-mediated microtubule (MT) nucleation has been

214 o consideration, we propose a model in which centrosomes migrate and are positioned through the conce

215 this orientation depends on a combination of centrosome movement controlled by Arp2/3-mediated regula

216 Centrosomes must resist microtubule-mediated forces for

217 ear envelope-based dynein pulling forces and centrosome-nuclear envelope tethering.

218 relationship between p15 and p16 expression, centrosome number abnormalities, and melanoma progressio

219 ormal human melanocyte lines did not exhibit centrosome number abnormalities, whereas those from late

220 e is strictly controlled to maintain correct centrosome number and spindle polarity in cells.

221 cellular processes, including regulation of centrosome number, primary cilium morphology, nuclear me

222 There is no apparent alteration in centrosome number, spindle formation or primary cilia, s

223 n occurs once in each cell cycle to maintain centrosome number.

224 How polyploid cells deal with increased centrosome numbers and DNA content remains unknown.

225 , accurate way to distinguish alterations in centrosome numbers at the level of single cells.

226 et spatially restricted Ca(2+) signal at the centrosomes of actively dividing cells.

227 orylated paxillin (Ser-272) was localized in centrosomes of human smooth muscle cells, which regulate

228 rematurely displaced DAs from the interphase centrosomes of immortalized retina pigment epithelial (R

229 monomer and polymer tubulin concentration at centrosomes of up to 660 uM.

230 oteins that localize to the centrosome (the "centrosome-ome").

231 A numerical increase in centrosomes, or centrosome amplification (CA), is common

232 During mitosis, centrosomes organize and nucleate the majority of spindl

233 cleated Dictyostelium discoideum cells, each centrosome organizes a radial MT network, and these netw

234 Nuclear movement and centrosome orientation were also defective in fibroblast

235 he cables necessary for nuclear movement and centrosome orientation.

236 n finding that near-tetraploids rapidly lose centrosomes over time.

237 block, p15 and p16 status determined whether centrosome overduplication would occur.

238 entrosome separation, leading to unseparated centrosome pairs dissociated from the nuclear envelope.

239 ce between the two), with the larger mitotic centrosome placed toward the embryo center in a polo-lik

240 Centrosomes play a pivotal role in regulating bipolar sp

241 Godinho investigates the role centrosomes play in cancer cell biology.

242 bule overgrowth and was sufficient to rescue centrosome polarization in KIF21B-knockout cells.

243 Centrosome polarization is thought to enhance the potenc

244 r data reveal that S6K1 hyperactivity alters centrosome positioning in mitotic cells, affecting orien

245 We present a model whereby centrosomes prevent inappropriate beta-catenin modificat

246 We show that CEP85L is a centrosome protein localizing to the pericentriolar mate

247 Here, we show that the depletion of centrosome proteins in NPCs prolongs mitosis and increas

248 the mother or daughter-centriole-containing centrosome, providing a possible mechanism for biased de

249 rticle protein complex (TRAPPC) proteins for centrosome recruitment during ciliogenesis.

250 EB1 depletion results in many centrosome-related defects.

251 re well conserved in different kingdoms, the centrosome, representing the most prominent microtubule

252 or-posterior axis specification is guided by centrosomes, resulting in anterior-directed cortical flo

253 hotopharmacology, we show that, in vivo, the centrosome's position relative to the nucleus is not lin

254 ctively confer strength and ductility to the centrosome scaffold so that it can resist microtubule-pu

255 In vitro, PLK-1 and SPD-2 directly protected centrosome scaffolds from force-induced disassembly.

256 s regulating centrosome asymmetry and biased centrosome segregation are unclear.

257 nesin-13 KLP-7/MCAK, resulting in incomplete centrosome separation at NEBD in AB but not P1.

258 KBD expression rescues ensc centrosome separation defects in NBs, but not the fast o

259 also known as MAP7) controls spindle length, centrosome separation in brain neuroblasts (NBs) and asy

260 wo-cell stage, the somatic AB cell initiates centrosome separation later than the germline P1 cell.

261 results add critical precision to models of centrosome separation relative to the nucleus during spi

262 NIMA-related kinase 2 (NEK2), a regulator of centrosome separation that is also elevated in taxane-re

263 lear lamina simultaneously further disrupted centrosome separation, leading to unseparated centrosome

264 over a role for PP2A-B55/SUR-6 in regulating centrosome separation.

265 d nuclear-envelope dynein density for proper centrosome separation.

266 rbations to determine the molecular basis of centrosome strength and ductility in C. elegans embryos.

267 LIS1 also localizes to the centrosome, suggesting that this organelle is key to the

268 ancer and provide a rationale for the use of centrosome-targeting therapeutic agents in treating thes

269 A common model proposes that the extra centrosomes that are typically acquired during tetraploi

270 to reveal the unique regulation of stem cell centrosomes that may contribute to asymmetric stem cell

271 coding the 367 proteins that localize to the centrosome (the "centrosome-ome").

272 Centrosomes, the main microtubule organizing centers (MT

273 Centrosomes themselves duplicate once per cell cycle, in

274 ranes while only truncated OCLN continues at centrosomes throughout neurogenesis.

275 CK1delta and CK1epsilon localize to centrosomes throughout the cell cycle, and in interphase

276 y is accompanied by reorientation of the CTL centrosome to a position beneath the synaptic membrane.

277 h represent novel players in actin-dependent centrosome to basal body conversion.

278 DK2-dependent increase in STIL levels at the centrosome to drive CA.

279 trosomal protein that localizes USP9X to the centrosome to stabilize STIL and promote centriole dupli

280 Here we show in mice that anchoring of the centrosome to the apical membrane controls the mechanica

281 appendages and disrupts the anchorage of the centrosome to the apical membrane, resulting in the diso

282 nein-mediated pulling forces that allows the centrosome to translocate past the nucleus.

283 of CPAP-tubulin interaction activates extra centrosomes to nucleate enhanced numbers of microtubules

284 ity in the C. elegans zygote, diffusing from centrosomes to the overlying cortex to phosphorylate yet

285 , we examined the functional contribution of centrosomes to Wnt signaling, beta-catenin regulation, a

286 se, rapid dynein-driven translocation of the centrosome toward the contact site leads to reorganizati

287 , causes microtubule overgrowth and perturbs centrosome translocation.

288 Deregulation in the number of centrosomes triggers tumorigenesis.

289 Before mitosis, centrosomes undergo maturation characterized by expansio

290 hange between chromosomes, microtubules, and centrosomes upon tension release during anaphase.

291 In HEK293 cells depleted of centrosomes we find that beta-catenin synthesis and degr

292 ous Wnt signaling components are enriched at centrosomes, we examined the functional contribution of

293 g, especially for complex organelles such as centrosomes, where a liquid-like structure would allow t

294 etric behavior of the mother versus daughter centrosomes, whereby the self-renewing stem cell selecti

295 nt site for MT nucleation in mitosis are the centrosomes, which are composed of two centrioles, surro

296 in cancer, results in the presence of extra centrosomes, which has been associated with chromosome i

297 Centrioles form the core of centrosomes, which organize the interphase microtubule c

298 Centrioles constitute the core of centrosomes, which organize the microtubule cytoskeleton

299 ed mechanism of CIN is the overproduction of centrosomes, which promotes tumorigenesis in various mou

300 ent labeling indicates that Nup188 populates centrosomes with newly synthesized protein that does not