ライフサイエンスコーパス: channel current

1 produced a substantial increase in potassium channel current.

2 tion of alpha1-A(R)s augments tonic GluD1(R)-channel current.

3 ormation that can lead to inhibition of GLIC channel current.

4 e channel sufficient to account for the open channel current.

5 evated membrane expression of BKalpha and BK channel current.

6 or NADP(+), induces a large increase in open channel current.

7 tion in cardiac repolarizing I(Ks) potassium channel current.

8 annel open-time, open probability, or single-channel current.

9 an increase in the size of the atypical Ca2+ channel current.

10 ost sensitively to changes in L-type calcium channel current.

11 ts NCLX activity, and impairs SOCE and Orai1 channel current.

12 pen probability without modifying the single channel current.

13 is was not accompanied by a change in Ca(2+) channel current.

14 nvestigating the effect of TNFalpha on TASK3 channel current.

15 uced calcium permeability and reduced single-channel currents.

16 mode to measure the effects of drugs on ion-channel currents.

17 e predictive of whether a ligand could evoke channel currents.

18 w that Delta27-264 CFTR can restore chloride channel currents.

19 Xenopus laevis oocytes elicited S-type anion channel currents.

20 ) release induced by increased L-type Ca(2+) channel currents.

21 activation kinetics of baclofen-induced GIRK channel currents.

22 arge-conductance, Ca(2+)-activated K(+) (BK) channel currents.

23 ilar differences are also seen in the single-channel currents.

24 balance in favor of D(s) and increasing ion channel currents.

25 ns, and the GBP-induced reduction in calcium channel currents.

26 pendent activation of inwardly rectifying K+ channel currents.

27 ase C deplete PIP(2) and reduce those Ca(2+) channel currents.

28 n yet together synergize to inhibit Ca(v)3.2 channel currents.

29 ity and increased constitutive and activated channel currents.

30 tion, nor did it affect voltage-activated Ca channel currents.

31 adder smooth muscle had no measurable K(V) 7 channel currents.

32 is lost, to make in situ recordings of nAChR channel currents.

33 either Orai1 or Orai3 markedly inhibits ARC channel currents.

34 ression of Orai1 increases both CRAC and ARC channel currents.

35 by the recording of maximally activated CRAC channel currents.

36 pparent reduction of the amplitude of single-channel currents.

37 antibodies induced the appearance of single-channel currents.

38 hannel-like structures and elicit single ion-channel currents.

39 ion (LoF) point changes leading to decreased channel currents.

40 ical because of the rapid rundown of calcium channel currents.

41 e individual showed markedly decreased NALCN channel currents.

42 hat has precluded direct recording of single-channel currents.

43 f hMSC families with distinct functional ion channel currents.

44 tor of high-voltage-activated N-type calcium channel currents.

45 in parallel with increased potassium (K(+)) channel currents.

46 f L-type channel currents compared to N-type channel currents.

47 ted abnormal SCN5A mRNA splicing reduced Na+ channel current 91.1+/-9.3% to a range known to cause su

48 in the model by inhibiting appropriate ionic channel currents according to experimentally reported co

49 individual experiment (i.e., measurement of channel currents across the endolysosomal membrane), inc

50 ed opening frequency and open time of single channel currents activated by maximally effective concen

51 We describe GluA3 single channel currents activated by nitrowillardiine (NO(2)W)

52 ficantly decreased Ca(2+)-dependent chloride channel currents activated in response to the nanoporati

53 d alpha-conotoxins Vc1.1 and RgIA on calcium channel currents after transient knockdown of the GABA(B

54 in a substantial enhancement of the calcium channel currents, albeit less than that produced by wild

55 leotides) results in a large increase in ion channel current, allowing accurate quantitation of the k

56 a toxin ProTx-II, which also inhibits sodium channel currents, altered the gating-pore currents in mu

57 -->O isomerization process; and 3), the open-channel current amplitude (i(0)), which reports whether

58 e or by homoquinolinate has identical single-channel current amplitude and mean open-channel duration

59 ased ENaC open probability (P(o)) and single-channel current amplitude but not the unit conductance.

60 With the reported method, single-channel current amplitude of native voltage-gated calciu

61 of -20 mV, we found that the average single-channel current amplitude was approximately 0.04 pA, inc

62 ied by a significant reduction of the single-channel current amplitude, indicating an interaction of

63 ide-out patches, but has no effect on single-channel current amplitude.

64 that GLT-1c and EAAT5 also differ in single-channel current amplitudes of associated anion channels.

65 tivation by combining mutagenesis and single-channel current analyses.

66 es were also functionally assessed by single channel current analysis.

67 function of Shank proteins is to regulate L-channel current and activity induced gene expression.

68 ased and decreased shn-1 gene dosage alter L-channel current and activity-induced expression of a CRH

69 a reduction in NPo of a 238 pS arterial KCa channel current and an increase in [Ca(2+)]i level in fr

70 tates in both cycles are responsible for the channel current and fit the current transients under the

71 It has been shown that the channel current and the conformational flexibility of co

72 channel proton current was smaller than open-channel current and was inhibited by 10 muM Zn(2+) Extre

73 tions and EAAT2/EAAT4 measurements of single-channel currents and anion/cation selectivities.

74 f Panx1 at Cys-40 and Cys-346 inhibits Panx1 channel currents and ATP release.

75 inic acetylcholine receptors can enhance ion channel currents and downstream effects of alpha7 stimul

76 We recorded single-channel currents and estimated the gating rate and equil

77 CAF +/- HAL are studied on RYR1 single-channel currents and HET myotubes to define molecular me

78 We simultaneously measured channel currents and nucleotide binding to Kir6.2.

79 ished both oxyhb-induced suppression of K(v) channel currents and oxyhb-induced constriction of isola

80 vel differential regulation of L-type Ca(2+) channel currents and SR load by GRK2.

81 sted that hyperpolarization-activated cation channel currents and T-type calcium channel currents may

82 bunits results in a graded reduction in CRAC channel currents and that this effect is independent of

83 pool in tsA-201 cells, we monitored KCNQ2/3 channel currents and translocation of PHPLCdelta1 domain

84 d G-protein-coupled inwardly rectifying K(+) channel current, and attenuated calcium current, and ind

85 esult of reciprocal actions on VRAC and KATP channel currents, and could contribute towards the stimu

86 s exhibit increased basal and GPCR-activated channel currents, and increased Ca(2+) fluorescence resp

87 pits, nearly complete inhibition of KCNQ2/3 channel currents, and loss of membrane ruffling.

88 ole-cell patch clamp was used to assess K(+) channel currents, and mRNA and protein expression was de

89 asing G-protein-gated inwardly rectifying K+ channel currents, and presynaptically inhibiting release

90 t the axon initial segment, attenuates Na(v) channel currents, and reduces the excitability of hippoc

91 and characterize their whole-cell and single-channel currents, and surface and total levels.

92 ressing TRPM8, elicited Ca(2+) responses and channel currents, and those were inhibited in the presen

93 GIRK (G-protein-gated inwardly rectifying K+ channel) current, and attenuated calcium current, and in

94 d-rectifying K+ channels and background (Cl- channel) current, and to a parallel loss in sensitivity

95 y was carried out to determine whether these channel currents are involved in the PGE(2)-induced sens

96 expression of Orai3 alone has no effect, ARC channel currents are specifically increased by expressio

97 in the E160R mutation, whole-cell and single-channel currents are still observed in both the presence

98 The measured transmembrane single channel currents are very high, rivaling those typically

99 lts from loss of a direct effect on the Ca2+ channel current, as shown in a transfected cell line dev

100 s and autocorrelation coefficients of single-channel currents, as well as by the predicted time cours

101 show that tatM2NX inhibits over 90% of TRPM2 channel currents at concentrations as low as 2 muM.

102 ealed a similar density of whole-cell Ca(2+) channel current between single smooth muscle cells (SMCs

103 dden Markov Models (MHMMs) to the problem of channel current blockade clustering and associated analy

104 i1 alone determine the magnitude of the CRAC channel currents, both Orai1 and the closely related Ora

105 the patch pipette solution blocked TRPC1/C5 channel currents but potentiated by about six-fold TRPC6

106 NADPH inhibits inactivation and potentiates channel current, but the mechanism behind this effect is

107 illustrated by the suppression of KCNQ K(+) channel current by activation of M(1) muscarinic recepto

108 Transient, ~95% blockage of the channel current by alpha-syn was observed when 1), alpha

109 EGTA reduced PC2hst channel currents by 86%, with a t1/2 = 3.6 min, whereas

110 atase (VSP) suppresses Ca(V)1.2 and Ca(V)1.3 channel currents by approximately 35% and Ca(V)2.1 and C

111 te, all mutations increase whole-cell K(ATP) channel currents by reducing channel inhibition by MgATP

112 t of silencing and permanent blockade of the channel current cannot necessarily be attributed to the

113 -activated cyclic nucleotide-gated (HCN) ion channel current characteristics.

114 m channels show a marked reduction of L-type channel currents compared to N-type channel currents.

115 conductance calcium-activated potassium (BK) channel currents contribute to action potential (AP) rep

116 ing for I409C/R410C, which in turn decreases channel currents, cross-linking of L402C/L403C is functi

117 The D540C-L666C channel current decreased in an oxidizing environment in

118 Whole cell K(ATP) channel current density (activated by metabolic inhibiti

119 s because of better-maintained L-type Ca(2+) channel current density and no increase in sodium-Ca(2+)

120 teroid hormone-induced up-regulation of BKCa channel current density and NS1619-mediated relaxations,

121 KCa channel beta1 subunit abundance and BKCa channel current density in uterine arteries from pregnan

122 howed that R222Q Nav1.5 did not alter sodium channel current density, but did left shift steady-state

123 sheep, hypoxia significantly inhibited BKCa channel current density, decreased NS1619-induced relaxa

124 We used real-time monitoring of K(ATP) channel current density, immunohistochemistry, and bioti

125 tein caused a significant increase in Ca(2+) channel current density, whereas lentivirus-mediated CRM

126 lta-1(-/-) DRG neurons exhibit lower calcium channel current density.

127 (v)1.5 membrane targeting, and reduced Na(+) channel current density.

128 duration but significantly depressed sodium channel current-dependent parameters causing a reduction

129 oving boundary electrophoresis (GEMBE), with channel current detection.

130 y of these compounds in potentiating the SK2 channel current determined by electrophysiological recor

131 Voltage-gated Ca(2+) channel currents determined by patch-clamp recording wer

132 sed in HEK-293 cells, the kinetics of single-channel currents differ only slightly, but they are subs

133 The ion channel current directly charges the quantum capacitance

134 CRAC-/STIM1-mediated Orai channel currents display characteristic hallmarks such a

135 t pharmacologic enhancement of mutated TASK3 channel current during development may, therefore, provi

136 ell-attached patch-clamp recording of single-channel currents elicited by 30-10000 microM glycine.

137 ted with decreased cardiac voltage-gated Na+ channel current (encoded by SCN5A), and the changes have

138 her depolarized, then the hyperpolarizing BK channel current exceeds its facilitatory effect, causing

139 wentoxin-IV (HWTX-IV), which inhibits sodium channel current, exclusively enhanced inward gating-pore

140 RTX-induced single channel currents exhibit multiple conductance states and

141 easure the activity of BK and GirK potassium channel currents expressed in Xenopus laevis oocytes.

142 dation is provided by injecting the designed channel current for arrhythmia termination in human atri

143 Single-channel currents for formate, hypophosphite and nitrite

144 his gating isomerization, we examined single-channel currents from adult mouse muscle AChRs that isom

145 eptor function by PbTx-2, we recorded single-channel currents from cell-attached patches.

146 ological evidence for the presence of single channel currents from isolated primary cilia of LLC-PK1

147 l signals, we recorded whole-cell and single-channel currents from mouse hair cells that expressed Tm

148 We studied single-channel currents from neuromuscular acetylcholine recept

149 ments to receptor gating, we recorded single-channel currents from outside-out patches containing a s

150 ring postnatal development, we recorded Ca2+ channel currents from Purkinje cells in cerebellar slice

151 Here we compare agonist-elicited single channel currents from receptors assembled with an excess

152 ntially distinguishing effects requiring ion channel currents from signaling induced by nonconducting

153 f adenylyl cyclase, stimulation of potassium channel currents (G(i)), and activation of phospholipase

154 mall-conductance calcium-activated potassium channel currents generate an apamin-sensitive medium AHP

155 Studying ion channel currents generated distally from the recording s

156 In TRPC1(-/-) VSMCs, CPA-induced channel currents had 3 subconductance states of 14, 32,

157 Single-channel currents had a unitary conductance of 210 pS, ty

158 yrate (PDBu) in inside-out patches and these channel currents had a unitary conductance of about 2 pS

159 tivated cyclic nucleotide-gated cation (HCN) channel currents have been identified in various periphe

160 Irregular voltage-dependent single-channel currents, high gating charges, and efficient dye

161 f the N-terminal EF hand transforms the CRAC channel current (I(CRAC)) into a constitutively active,

162 used to evaluate inactivation of peak sodium channel current (I(Na)) in myocytes isolated from canine

163 inactivation, and because facilitation of Ca-channel currents (I(Ca)) masks the extent and kinetics o

164 t it did inhibit Ca(V)2 voltage-gated Ca(2+) channel currents (I(Ca)).

165 bath application of 10 nm ET-1 evoked cation channel currents (I(cat)) with subconductance states of

166 tions result in the potent inhibition of Nav channel currents (I(Na)) and in changes in the voltage d

167 There was no change in L-type Ca2+ channel current (ICa) density but the ability of ICa to

168 er IL-6 reduced Ca2+ influx via L-type Ca2+ -channel current (ICa,L).

169 llodynia by modulating voltage-gated calcium channel currents (ICa(V)).

170 ta sets on AF-induced changes of major ionic channel currents (ICaL, IKur, Ito, IK1, IKs, INaCa) and

171 EA induced cation channel current (Icat) in SW982 cells with biophysical c

172 entrations of Ang II (1 nM) activated cation channel currents (Icat1) with conductances states of abo

173 Painful axotomy decreases K(ATP) channel current (IK(ATP)) in primary afferent neurons.

174 s but no significant changes in transient K+-channel current (IKA) amplitude.

175 conductance, we measured Shaker-P475D single-channel current in a wide range of symmetrical K(+) conc

176 e produced greater inhibition of peak sodium channel current in atrial cells isolated from HF versus

177 Here, we recorded the voltage-gated calcium channel current in nucleated patches from layer 5 pyrami

178 ane stretch suppresses an XE991-sensitive Kv channel current in patch-clamped vascular smooth muscle

179 described by the model: the ratio of single channel current in the presence of PEG to that in the po

180 The amplitudes of their single-channel currents in a physiological concentration of ext

181 potently inhibits the Abeta-induced calcium channel currents in artificial lipid membranes.

182 ) vesicles, but failed to inhibit single RyR channel currents in bilayers.

183 wever, mAb 131 did not reduce fetal AChR ion channel currents in electrophysiological experiments.

184 DQP-1105 inhibited single-channel currents in excised outside-out patches without

185 By action spectroscopy of photoinduced channel currents in HEK293 cells and absorption spectros

186 cordings of both macroscopic and single dSlo channel currents in identified neurosecretory neurons in

187 ns in the properties of voltage-gated sodium channel currents in Jedi-1 null neurons.

188 in ex vivo slices significantly reduced KCa2 channel currents in layer V IL-PFC pyramidal neurons, co

189 tein-activated inwardly rectifying potassium channel currents in mouse brain slices containing locus

190 namic interaction among Kir2, Kleak, and HCN channel currents in shaping membrane potential and the t

191 effects of a methanethiosulfonate reagent on channel currents in single and double Cys mutants, we id

192 l tools to dissect the various kinds of K(+) channel currents in situ.

193 i and a beta-subunit antibody) abolished Kv7 channel currents in the absence of either Gbetagamma sub

194 GABARs in HEK 293 cells and recorded single channel currents in the cell-attached configuration usin

195 cules to examine their ability to induce ion channel currents in the whole spermatozoa.

196 KCNQ channel currents in these cells were potently reduced by

197 also ablated the effects of hypoxia on BKCa channel currents in uterine arteries.

198 sible hidden Markov model for the underlying channel current, in which successive measurements of the

199 ncreases linearly as the amplitude of single channel currents increases, while the open time for inwa

200 f the membrane to differentially enhance ERG channel currents independent of channel state.

201 tion of S-acylation attenuated endogenous BK channel currents independently of changes in cell surfac

202 By comparing whole-cell and single channel currents induced by the agonists, we provide a m

203 We hypothesized that Cav3.1 T-type Ca2+ channel currents inhibit thalamic sensory transmission t

204 Electrophysiological recording of single-channel currents is the most direct method available for

205 ChRs tested the Lys-132 homolog also alters channel current kinetics.

206 ed VZV isolates induce changes in sodium ion channel currents known to be associated with neuropathic

207 Calcium influx activates the BK channel current, leading to potassium efflux that increa

208 d cation channel currents and T-type calcium channel currents may account for some of the membrane pr

209 similar mutation-induced alterations in IKs channel currents may be caused by different molecular me

210 ta2gamma2L and alpha6(R46W)beta2delta single-channel current mean open times and burst durations were

211 Thus single channel current measurements reveal bases for the distin

212 Single-channel current measurements show that the channel funct

213 ted FETs, we have demonstrated that the high channel currents obtained at low gate voltages result fr

214 nherent randomness of telegraph noise in the channel current of a single CMOS transistor as an entrop

215 method that allows measurement of the single-channel current of ion channels even when it is too smal

216 choline (ACh) binding and gating from single channel currents of fetal mouse AChRs expressed in tissu

217 A kinetic analysis of spontaneous channel currents of PC2 showed that four intrinsic, non-

218 Single-channel current openings were on average 32% shorter in

219 not due to changes in voltage-gated calcium channel currents or activation kinetics.

220 By dramatically suppressing channel currents, PIP3 may generate a shift in odorant s

221 e predictive of a ligand's ability to induce channel currents points to these conformational biosenso

222 old mice also exhibit smaller L-type calcium channel currents, providing a plausible mechanism that l

223 d memory window (>180 V), a high erase/write channel current ratio (10(4) ), good data retention, and

224 r voltage clamp, and the ensemble of the ion channel currents reconstructed in silico generates rhyth

225 e, consistent with the increased fast sodium channel current recorded from BACE1-null hippocampal neu

226 used kinetic analyses of equilibrium single-channel currents recorded from individual GluN1/GluN2A r

227 On the basis of single-channel currents recorded from the muscle nicotinic acet

228 By using single-channel current recording, we define discrete assembly i

229 Furthermore, we demonstrate using channel current recordings and binding assays that OAADP

230 mbrane binding and discuss how to use single-channel current recordings and dye flux assays to confir

231 In vitro hERG channel current recordings are an important step in eval

232 Functional studies using single channel current recordings of the sensorless pore module

233 Further, from one-channel current recordings, we found that 4 mM NPA cause

234 e and alter the direction and amount of open channel current rectification.

235 of ADPRase activity fails to affect nvTRPM2 channel currents, reporting a catalytic cycle uncoupled

236 pe Arg83 and variant Gly83 ClC-K(a) chloride channel currents revealed approximately 50% loss-of-func

237 y decreasing the calcium-activated potassium channel current (SK), as well as elevates dopaminergic n

238 We have measured single-channel currents, spontaneous gating, and rectification

239 In addition, we show that multi-channel, current-steering-based stimulation within the c

240 ed Tmc1 and Tmc2 had a broad range of single-channel currents, suggesting multiple heteromeric assemb

241 cytes show hyperactivation of L-type calcium channel current that could not be reversed with palmitat

242 utward-directed current precedes the passive channel current that has the opposite direction at physi

243 K(+) channel current that normally mediates K(+) uptake for s

244 -1)) all caused a 20% increase in maxi-K(Ca) channel current that was blocked by AG-1478 or by knock-

245 We propose that smaller ion channel currents that contribute to setting the resting

246 ed large-conductance, Ca2+-sensitive K+ (BK) channel currents that could be activated by neuronal sti

247 aracterization of prolonged bursts of single-channel currents that occur with PNU-120596 provide a re

248 We estimated (from single-channel currents) the components of energy for gating ar

249 ous alpha5beta1 integrin ligand, enhances BK channel current through both Ca(2+)- and phosphorylation

250 Whole-cell K(ATP) channel currents through homomeric and heterozygous F35V

251 potentiation, we examined ACh-induced single-channel currents through the human neuronal alpha7 acety

252 presence of tRNA, blocking events of single-channel currents through the pore formed by recombinant

253 aveolae by MCD leads to a decrease in the BK channel current thus increasing cell excitability, and (

254 his form of crosstalk requires L-type Ca(2+) channel current to activate STIM1, and betaAR activity e

255 We use the average channel current to create a conductance image and the ra

256 ifies oscillatory dendritic Ca(2+) and Na(+) channel currents to lead to the initiation of high-frequ

257 PA1) into lipid bilayers and recorded single-channel currents to understand its inherent thermo- and

258 he requirement of calcium-activated chloride channels currents to mediate pheromone activation.

259 ucing plausible reaction schemes from single-channel current traces is time-consuming and difficult.

260 ed, which led to the proposal that gA single-channel current transitions result from closed-open tran

261 (anion) in Vicia faba guard cells, measuring channel current under a voltage clamp while manipulating

262 resolution structures of several prokaryotic channels, current understanding of their physiological r

263 ovalent modulation of macroscopic and single-channel currents using extracellularly and intracellular

264 um antibodies and their effects on potassium channel currents using human embryonic kidney cells expr

265 bition we recorded wild-type and mutant CFTR channel currents using inside-out membrane patches from

266 gests that mutant MEC-10 proteins may affect channel current via MEC-2.

267 Single channel current-voltage curves are computed over hundred

268 Single-channel current-voltage relationships were nonlinear in

269 outside-out patches showed that in all Kir2 channels current-voltage relationships display a 'crosso

270 SK channel inhibitors, while expressed IKCa channel current was inhibited by known IKCa channel bloc

271 We found that at P40-50, the somatic Ca2+ channel current was inhibited by omega-agatoxin IVA at c

272 function, and the reduction in the potassium-channel current was remedied by the application of the p

273 ximal activation rate of ensembled single BK channel currents was in the submillisecond range at > or

274 Using kinetic modeling of one-channel currents, we attributed the observed current dec

275 In detached, inside out patches single-channel currents were abolished by [Au(CN)2]- and activi

276 and alpha4beta2delta(R220H) receptor single-channel currents were both significantly decreased compa

277 tivation and the deactivation rate of mutant channel currents were characterized using the two-microe

278 KcsA channel currents were effectively blocked by potassium s

279 Channel currents were identified as K(ATP) channels thro

280 DiC8-PIP(2)-evoked channel currents were inhibited by anti-TRPC1 antibodies

281 RyR2 channel currents were measured in planar lipid bilayers.

282 BK channel currents were measured using whole-cell patch cl

283 The channel currents were recorded under steady-state condit

284 ious work has suggested that subthreshold K+ channel currents were responsible for this dichotomous b

285 alpha9alpha10 nAChRs but not N-type calcium channel currents, whereas [2,8]-dicarba analogues displa

286 operty of HCN channels is their small single-channel current, which is below the thermal noise thresh

287 ions in this gene produced reduced potassium-channel current, which was successfully remedied by phar

288 ',N'-tetraacetic acid (BAPTA-AM) both evoked channel currents, which had unitary conductances of appr

289 onstrated that D1R stimulation increased HCN channel current, while local HCN channel blockade in pri

290 tro recordings tested for D1R actions on HCN channel current, while recordings in monkeys performing

291 d high calcium permeability and large single-channel currents, while cells with mutant Tmc1 had reduc

292 d a concentration-dependent inhibition of BK channel currents with 3 mM producing approximately 50% i

293 o quiescent inside-out patches evoked single channel currents with a unitary conductance of 1.9 pS.

294 ET-1 evoked non-selective cation channel currents with a unitary conductance of 2.6 pS wh

295 e extracellular solution also reduced single-channel currents with an affinity that increased as [K(+

296 8810 (FP receptor antagonist)-insensitive BK channel currents with EC(50)s of 0.51 +/- 0.03 nM (n = 5

297 We examined GluN1/GluN2A single-channel currents with kinetic analyses and modeling to p

298 AMPAR/TARP combinations gave rise to single-channel currents with multiple conductance levels that a

299 racellular Ca2+ stores also activated cation channel currents with similar properties to Icat2.

300 the microsomes harboring KcsA showing single-channel currents with the typical biophysical characteri