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1 Hofmeister series, also labeled by the term chaotropic.
2 We also reveal inconsistent measurements of chaotropic activity in the literature highlighting the n
3 from hydrophobic substances that exert their chaotropic activity, by proxy, from within the hydrophob
4 buffer with a fixed optimal concentration of chaotropic agent (e.g., 1.2 M urea) to differentially is
6 n vitro, such as elevated temperatures and a chaotropic agent guanidine hydrochloride, were studied.
8 was found to play a crucial role acting as a chaotropic agent that disrupts undesired H-bonding inter
11 ions, including GuH(+), which also acts as a chaotropic agent, affect the equilibrium between the ope
12 as a cation, stimulating activity, and as a chaotropic agent, altering the distribution of conformat
13 chanistic role of guanidinium thiocyanate, a chaotropic agent, in the crystallization of Sn-Pb perovs
14 Guanidine hydrochloride (GdnHCl), a strong chaotropic agent, influences nanoparticle stability and
15 autosampler at 5 degrees C and (b) a strong chaotropic agent, such as guanidine hydrochloride, is cr
16 in-lacking mitochondria in the presence of a chaotropic agent, which disrupts the interaction between
18 and 31 nM in the absence or presence of anti-chaotropic agents (10% glycerol and 0.5 M Na2SO4), respe
19 lvents (acetonitrile, methanol, 2-propanol), chaotropic agents (6 M urea, 6 M guanidine-HCl), a prote
22 from isolated tonoplast preparations by mild chaotropic agents and thus appeared to be nonintegrally
23 -resistant oligomers and filaments stable to chaotropic agents are detected using all Tyr --> Phe alp
26 60-fold stabilization of the dimer, the anti-chaotropic agents produced a 7-fold enhancement in the c
27 ructive heat denaturation in the presence of chaotropic agents such as formamide to allow access to t
28 itro method to desaturate LH-RH receptors by chaotropic agents such as manganous chloride (MnCl2) and
31 ronment (changes in temperature, addition of chaotropic agents), no isolated subunits in native condi
34 nanodiamonds and separating out detergents, chaotropic agents, and salts, and other impurities that
35 mbranes after treatment with Triton X-100 or chaotropic agents, implying a stabilizing role for PsaD.
37 complex mixture of inorganic salts, buffers, chaotropic agents, surfactants/detergents, preservatives
41 in the Y42F mutant can be interconverted by chaotropic and kosmotropic agents, respectively, accordi
42 glutamate aminopeptidase requires the use of chaotropic and reducing agents, quite often with limited
43 temperatures and polysorbate 20, rather than chaotropic and reducing agents, with sample cleanup and
45 are often touted for their stability to both chaotropic and thermal challenges, fuscanodin is found t
47 iated less than were glial currents when the chaotropic anion thiocyanate was substituted for glucona
49 of catecholamine molecules is released after chaotropic anions (ClO(4)(-) and SCN(-)) treatment, even
52 lting in strong binding between proteins and chaotropic anions and between nucleic acids and kosmotro
55 e, we present the first direct evidence that chaotropic anions have an affinity for hydrophobic conca
56 tion can be controlled by the application of chaotropic anions in the mixture, allowing a second leve
58 d for recycling of Em is activated by Pi and chaotropic anions such as azide and thiocyanate, giving
59 These transporters exhibit a permeability to chaotropic anions that is associated with transport, all
62 Analyses of cells treated with the highly chaotropic bile salt detergent deoxycholic acid demonstr
63 r high potential for biomarker preservation, chaotropic brines could therefore serve as repositories
64 enous genetic material is preserved in these chaotropic brines, producing an unexpected increase in d
68 , 'hyperfolder YFP' (hfYFP), that withstands chaotropic conditions that denature most biological stru
69 elemental bricks" under certain detergent or chaotropic conditions, but our companion paper provides
72 of proteins in two case studies using (i) a chaotropic denaturant (urea) and (ii) low-pH buffers use
73 ermore, treatment of pure protein with acid, chaotropic denaturants, alkylators, and detergents faile
74 The modified peptide is more resistant to chaotropic denaturants, and the free energy of folding o
75 However, the "end-states" of temperature and chaotropic denaturation are spectroscopically differenti
78 well fitted as a sum of a linear, presumably chaotropic effect and a square root-dependent, presumabl
79 e spectroscopic probe of the ion kosmotropic/chaotropic effect at aqueous interfaces as well as of th
80 n, we overcome these problems by introducing chaotropic effects in SFC separations using a nontraditi
82 xigenic Escherichia coli (ETEC) toxins after chaotropic extraction of DNA from stool would increase t
83 but there are key points that delineate (i) chaotropic from kosmotropic substances (i.e. chaotropes
87 nanochannel structure demonstrated that the chaotropic ion results in the smallest nanochannel size
88 arried out in the presence of thiocyanate, a chaotropic ion that markedly increases the affinity of A
89 enhance cation exclusion effects and reduce chaotropic/ion pairing effects by using (1) low ionic st
90 (dihydrogen phosphate) that exhibits minimal chaotropic/ion pairing effects, and (3) columns that sho
91 c (HPO(4)(-2), SO(4)(-2), and CO(3)(-2)) and chaotropic ions (NO(3)(-) and I(-)) to determine their i
92 simulations and experiments have shown that chaotropic ions actually exhibit enhanced concentrations
93 is to study the behaviour of kosmotropic and chaotropic ions in aqueous solutions of polyhydroxy comp
97 with the cationic Hofmeister hierarchy, the chaotropic Mg(2+) ions caused the greatest thermal desta
99 daptations, it suggests that the presence of chaotropic molecules may be beneficial to life operating
100 th a modification of the recently introduced chaotropic NaI method, reducing our estimate of the leve
102 investigated the effects of either strongly chaotropic, nearly neutral or strongly kosmotropic salt
103 ly describe the chemical nature (kosmotropic/chaotropic or hard/soft) of the interacting species.
106 e that this behavior is consistent with the "chaotropic" or "structure breaking" model of the hydrati
108 Herein, we exploit the affinity between a chaotropic POM and native cyclodextrins (alpha-, beta-,
109 MCF-7 cells also showed the requirement of a chaotropic protein denaturant in the assays and selectiv
110 poisoning by ICRF-193 required the use of a chaotropic protein denaturant in the topoisomerase poiso
112 alovirus (CMV) antigen preparations with the chaotropic reagent Urea and probing with immune sera fro
113 n of reaction time, pH, organic solvent, and chaotropic reagents (urea, guanidine HCl) by monitoring
115 h-speed cell disruption (HSCD) incorporating chaotropic reagents and lysing matrices in comparison to
117 ding or by denaturation of the bound Ig with chaotropic reagents, but could be dissociated by reducti
119 ing kinetics is more complex when the highly chaotropic salt guanidine hydrochloride (GuHCl) is emplo
120 e from the endoplasmic reticulum membrane by chaotropic salt wash, consistent with lack of a putative
122 i) the use of PCR-inhibiting reagents (e.g., chaotropic salts and alcohol) and (ii) the washing and e
123 ncubated with perchlorates, highly oxidizing chaotropic salts which have been found across the Martia
124 lly used in DNA extraction procedures (e.g., chaotropic salts, 2-propanol) can be avoided, making the
126 is quite similar to that in the presence of chaotropic sodium bromide (and other halides), which rai
127 otropes </= -4 kJ kg(-1) mol(-1) ); and (ii) chaotropic solutes that are readily water-soluble (log P
128 CFU/ml, respectively, for a glass matrix and chaotropic solution protocol; 8.0 x 10(2) and 7.0 x 10(3
129 y MS showed that FCGBP was mainly soluble in chaotropic solutions, whereas MUC2 was insoluble, and mo
130 d the effect of acid and other denaturing or chaotropic solvents on the conformation of monoclonal an
131 e sequential use of these three increasingly chaotropic solvents to elute antibodies results in both
134 es water activity (a(w) ) and exerts extreme chaotropic stress, the tendency of a solution to disorde
135 alternative methods tested, the use of this chaotropic technique of DNA isolation by using NaI produ
136 Here, we characterize the adsorption of the chaotropic thiocyanate anion (SCN(-)) to two prototypica
137 nic generation (SHG) studies of the strongly chaotropic thiocyanate ion adsorbed to the interface for
139 mbrane-bound VP14 was partially resistant to chaotropic washes such as 0.1 M Na2CO3 (pH 11.5) and 6 M
140 ions favor native protein structure, whereas chaotropic (weakly water binding) cations enhance native