ライフサイエンスコーパス: charged residue

1 static interactions between a cation and key charged residues.

2 and d that are mediated by highly conserved charged residues.

3 including a repulsive stretch of positively charged residues.

4 o a high number of positively and negatively charged residues.

5 enced by the linear patterning of oppositely charged residues.

6 ontent, intermediate hydrophobicity, and few charged residues.

7 duction pathway lined by numerous positively charged residues.

8 osphosites that are surrounded by negatively-charged residues.

9 otential PKA site at Ser-938 and surrounding charged residues.

10 ormational changes in a network of conserved charged residues.

11 t concentration, due to Coulomb repulsion by charged residues.

12 m) abolished CaM binding without introducing charged residues.

13 ast, the same region comprises 17 positively charged residues.

14 ntribute to the surface potential with their charged residues.

15 ternary epitope containing a high density of charged residues.

16 by a linear fit to the density of positively charged residues.

17 that include both positively and negatively charged residues.

18 groove that is lined with several positively charged residues.

19 on of the DNA phosphate groups by positively charged residues.

20 inked to the fourth channel by a 'funnel' of charged residues.

21 linear sequence distributions of oppositely charged residues.

22 y, mediated by a set of conserved positively charged residues.

23 near mixing versus segregation of oppositely charged residues.

24 e hydration of nonpolar residues neighboring charged residues.

25 rminal PYD, and it is enriched in positively charged residues.

26 only on net charge, but also on the type of charged residues.

27 s of linker mutants revealed that a group of charged residues, (200)EKR(202), is crucial for the swiv

28 tic interactions with neighboring negatively charged residues, a result also confirmed by disulfide t

29 Thus, reversal of charged residues about the conserved tropomyosin-binding

30 Controls where lysines were mutated with charged residues accessed similar morphologies but did n

31 + T rich genome may influence how positively-charged residues accumulate in SLiMs.

32 els and enzymes by transporting electrically charged residues across a hydrophobic VSD constriction c

33 ostatic interactions between complimentarily charged residues across the interface between the N- and

34 ostatic interactions between complementarily charged residues across the interface between the N- and

35 Results confirm that charged residues adjacent to phosphorylation sites and r

36 Positively charged residues adjacent to phosphorylation sites in th

37 id binding of ATG16L1 by mutating negatively charged residues adjacent to the lipid binding motif als

38 esis to probe the functional significance of charged residues adjacent to the phosphorylation sites a

39 in this C-terminal tail balances positively charged residues, allowing a more compact ensemble of st

40 protein molecules in large ESI droplets-the charged residue and chain ejection models can possibly c

41 rstood that interactions between an embedded charged residue and other charged or polar residues in t

42 h the N-terminal sensor of BovK, and several charged residues and a conserved hydrophobic region in t

43 The movement of certain charged residues and a decrease in the distance between

44 two transmembrane helices contain conserved charged residues and are implicated in substrate binding

45 se sites are highly enriched in prolines and charged residues and are strikingly similar to other lig

46 ism that excludes substrates with positively-charged residues and favors LAT and SLP-76 phosphosites

47 a balance between the numbers of positively charged residues and hydrophobic anchoring residues.

48 unusual protein contains numerous polar and charged residues and lacks characteristic membrane-inter

49 We identify five charged residues and six uncharged residues at the inter

50 mportance of the rarely occurring negatively charged residues and the N-terminal coil region in tropo

51 e site contains several conserved positively charged residues, and a portion of the active site shows

52 ng a mix of bulky hydrophobic and positively charged residues, and an adjacent amphipathic region tha

53 s shows consensus proteins to be enriched in charged residues, and rarified in uncharged polar residu

54 Almost all proteins contain charged residues, and their chain distribution is tailor

55 H-NS N-terminal domain are unusually rich in charged residues, and their interaction is mostly electr

56 Once the charged residues are capped with CEs, certain charge sta

57 Charged residues are essential for maintaining the arran

58 t in the SH3-like domain of NdhS, where nine charged residues are highly conserved among plants.

59 chanism becomes YidC-dependent if negatively charged residues are inserted into the translocated peri

60 (kappa31) and kappa14, where the oppositely charged residues are more evenly distributed, exhibit a

61 quence of the linker reveals that positively charged residues are separated with a typical helical pe

62 estrict DNA interactions with the positively charged residues Arg-14 and Lys-17.

63 that substitution of a conserved positively charged residue (Arg-388, hEAAT1) in transmembrane domai

64 We have identified two charged residues (Arg(145) and Lys(153)) within this seg

65 Single alpha-helix (SAH) domains are rich in charged residues (Arg, Lys, and Glu) and stable in solut

66 how that the weaker densities for negatively charged residues arise from their greater sensitivity to

67 ne contains almost three times the number of charged residues as the internal side of the outer membr

68 le side chain of Trp-1126 and the negatively charged residues Asp-1129, Asp-1131, and Asp-1133.

69 Charged residues Asp-997, Glu-998, Arg-1000, and Lys-100

70 d that the activation process is hindered by charged residue associations as well as by local steric

71 However, a negatively charged residue at position 180 was necessary to convey

72 In contrast, substituting a negatively charged residue at the equivalent position in Kir7.1 enh

73 Mutation of a charged residue at the interface (Arg-103) weakens the i

74 replicase interaction requires a positively charged residue at the third position (3R) in the N-term

75 ng this mutant demonstrate that a negatively charged residue at this position is essential for normal

76 lity appeared to require a second positively charged residue at VP1-109.

77 Absence or presence of specific charged residues at particular positions makes EcYfdC se

78 Furthermore, the introduction of positively charged residues at position 345 rendered shifted biphas

79 tivity have been generated by elimination of charged residues at the A1-A2 and A2-A3 interfaces.

80 The presence of a pair of oppositely charged residues at the aforementioned positions in beta

81 identified the importance of four positively charged residues at the base of the cysteine-rich head a

82 sitively charged patch to bind to negatively charged residues at the C-termini of P-proteins.

83 ing and site-directed mutagenesis identified charged residues at the CD4-Env interface and clashes be

84 o membrane-protein insertion from positively charged residues at the cytoplasm-membrane interface and

85 Previously, charged residues at the edge of the LPS binding pocket h

86 Here, we determined the role of the charged residues at the end of TM-1 in voltage sensing i

87 n the amino acid sequence, we also show that charged residues at the fibril three-fold apices provide

88 ggregate, including enrichment of gatekeeper charged residues at the flanks of hydrophobic aggregatio

89 e analysis of the photoadducts revealed that charged residues at the N-terminus of the signal sequenc

90 Mutations of charged residues at the rim of Hfq interfered with posit

91 ences of the linear patterning of oppositely charged residues because it measures and separates prote

92 Exposure of hydrophobic (respectively charged) residues can lead to disk (respectively ring) d

93 Our ZIP2 models revealed a cluster of charged residues close to the substrate-translocation po

94 The present study evaluated the role of charged residue clusters in the regulation of MMP collag

95 cted at a limited subset of highly conserved charged residues, combined with ODN screening to elimina

96 although electrostatic interactions between charged residues contribute significantly to the overall

97 eport, we demonstrate that distal positively charged residues contribute to substrate binding in a sy

98 LSus of adjacent L2 dimers, where negatively charged residues coordinate around a Mg(2+) ion in a fas

99 model, confirmed that each of the positively charged residues critical for sweetness is close to a re

100 The ten AMPs contain five or six positively charged residues (d-Arg, d-Lys, d-Orn, l-Dab, or l-Dap)

101 This study confirmed the importance of the charged residues D219 and E220 in maintaining structural

102 ppressor analysis suggests that one of these charged residues, D87, has distal influence on interhexa

103 he presence of neutral instead of positively charged residues did not interfere with POMP10 localizat

104 The fraction of charged residues discriminates between weak and strong p

105 arity of the sulfate moieties on the GAG and charged residues displayed on the fibril surface.

106 he distribution of tryptophan and positively charged residues distinguishes them from non-LD-targetin

107 e, from the (19)F-NMR analysis two important charged residues, E7 and R28, were found to be positione

108 ing three pairs of positively and negatively charged residues (either Glu(-)/Arg(+), Asp(-)/Arg(+), o

109 e majority of the binding free energy, while charged residues elsewhere are less critical for binding

110 been hindered by their atypical aromatic and charged residue-enriched sequences, extreme sensitivitie

111 It is clear that charged residues exert significant influence over struct

112 binding mode further enables recognition of charged residues flanking the nascent TMD and thus expla

113 d the importance of the conserved positively charged residue for the function of the Escherichia coli

114 ng suggested the critical involvement of two charged residues for the interaction of PAI-1 with LRP1

115 the interaction of PAI-1 with LRP1 and three charged residues for the interaction of uPA:PAI-1 comple

116 packing interactions, whereas the positively charged residues form an exterior polar shell.

117 Varying the identity of charged residues (FRFEFRFE and FRFDFRFD) produced simila

118 rge zipper with interdigitated complementary charged residues from Hha and the two units of the H-NS

119 ains a cluster of three conserved negatively charged residues Glu-179, Asp-180, and Asp-181 that coul

120 ctrostatic and dipolar effects caused by the charged residues (Glu113, Glu181) and to strong hydrogen

121 Mutagenesis of Cys-rich domain-charged residues had no major effect on ADAMTS13 functio

122 dioxygen, demonstrating that the positively charged residue (His548) plays a significant role in cat

123 helmingly located in the vicinity of E929, a charged residue in a hydrophobic position of the heptad

124 rged residue in TRBV4-1 and a key negatively charged residue in CD1b that is shared with CD1c, which

125 We examine the role of Lys-377, the only charged residue in helix XI, on the functional mechanism

126 Each of these DDR events requires a charged residue in RNF168 (R57).

127 d predominantly to those having a negatively charged residue in the -2 position relative to the aspar

128 dies establish why mutations in a positively charged residue in the cationic pocket of an activation

129 The rationale was that a charged residue in the drug-binding pocket would disrupt

130 The sole negatively charged residue in the first half of human tropoelastin

131 Two mutations affecting the same positively charged residue in the S4 domain of K(V)7.2 have been fo

132 el family, TRPV5 and TRPV6 lack a positively charged residue in the TM4-TM5 loop that was shown to in

133 tational studies identified a key positively charged residue in TRBV4-1 and a key negatively charged

134 complex indicated that vCCI uses negatively charged residues in beta-sheet II to interact with posit

135 tic and biochemical approaches, we show that charged residues in Csm3 facilitate its self-assembly an

136 By mutagenesis, we tested the roles of charged residues in each putative permeation pathway.

137 -1 further confirm the critical roles of the charged residues in eotaxin-1.

138 d with 87 residues in HP1b) is critical; the charged residues in HP1a are necessary for tight peptide

139 on-binding residue in GlcP(Se) and show that charged residues in its vicinity affect the pK(a) of glu

140 completed the functional analysis of all 57 charged residues in MurJ and demonstrated that the respe

141 ic residues in subsite S2 and for positively charged residues in S1.

142 lysis, we find that the conserved positively charged residues in S4 are stabilized by countercharges

143 Either positively or negatively charged residues in that position completely blocked GCA

144 In addition, hydrophobic and charged residues in the 104 through 125 segment, arrange

145 stic role of key hydrophobic and hydrophilic/charged residues in the assembly of active LRRK2.

146 e polar residues to interact with positively charged residues in the C-terminal domain.

147 luster (by nitrosylation) permits positively charged residues in the C-terminal helix to engage in DN

148 From these data, we hypothesize that some charged residues in the cavity region of MurJ homologs a

149 Previous studies showed that 8 charged residues in the central cavity region of MurJ ar

150 Mutation of conserved charged residues in the deltaretrovirus bovine leukemia

151 also found that, upon neutralization of the charged residues in the first turn of M2, the control of

152 ype Pin1 WW domain, which has two positively charged residues in the first turn, was compared to the

153 We have now defined the contributions of charged residues in the FL region of the Rous sarcoma vi

154 charged residues within the alpha repeat and charged residues in the flexible gating loop of the cata

155 Next, we identified multiple positively charged residues in the inner ring of HUS1 that were cru

156 , suggesting the involvement of two critical charged residues in the interaction of fVIII with LRP1.

157 hannel opening, and is enabled by negatively charged residues in the linker region.

158 demonstrates the requirement for negatively charged residues in the loop regions for divalent ion bi

159 viral fusion by interacting with negatively charged residues in the MERS-CoV FP region.

160 in beta-sheet II to interact with positively charged residues in the MIP-1beta N terminus, 20s region

161 Furthermore, the positively charged residues in the N-terminal domain of VDAC1 inter

162 of NCp7 mutants, the presence of positively charged residues in the N-terminus was found to be essen

163 s and shown to be comprised of complementary charged residues in the NCAM Ig2 domain (Arg-156 and Lys

164 ces in the number and position of positively-charged residues in the outer sides of the junction bind

165 es, the distance and order of the oppositely charged residues in the peptide sequence differ, such th

166 for pairwise interactions between oppositely charged residues in the receptor and chemokine, confirmi

167 rine-threonine-rich sequence with positively charged residues in the RelA NTD.

168 rated without altering the invariant ring of charged residues in the selectivity filter that governs

169 This suggests that charged residues in the translocated domain and the hydr

170 s, revealing that ionic interactions between charged residues in the transmembrane domains of RNF170

171 ing transport, and a proton-relay network of charged residues in the transmembrane region is linked t

172 observed and correlated with an asymmetry in charged residues in the vicinity of the inner and outer

173 Two negatively charged residues in these networks are putative Ca(2+) b

174 Several positively charged residues in this interface were identified from

175 anslocated peptide region is lowered and the charged residues in this region are removed, translocati

176 Mutating charged residues in this region of Spt5 did not prevent

177 ctively demonstrate that removing conserved, charged residues in TNT1's tropomyosin-binding domain im

178 The protonation state of embedded charged residues in transmembrane proteins (TMPs) can co

179 Abeta oligomers is influenced by positively charged residues in two sites (positions 23-31 and 95-10

180 ria, we explored the conservation of these 8 charged residues in YtgP, a homolog from Streptococcus p

181 nsistent with the suggestion that positively charged residues interact with the negatively charged ri

182 smembrane segment 4 (S4) contains positively charged residues intercalated by hydrophobic residues.

183 oop and coincides with a patch of positively charged residues involving arginines 102, 104, 106, and

184 tein studies, we confirmed that a positively charged residue is a SecYEG determinant for the endogeno

185 le factors in tuning the pKa of TMP-embedded charged residues is important for bioengineering and bio

186 appa56 with linear segregation of oppositely charged residues leads to limited conformational heterog

187 Increased segregation of oppositely charged residues leads to linear decreases in the global

188 that local clustering of hydrophobic and/or charged residues leads to significant collapse of the un

189 nctional studies demonstrating that specific charged residues localized in the central cavity are ess

190 l, charges on the S4 segment are screened by charged residues localized on neighboring segments of th

191 '-AMP-PPi moiety is guided by the positively charged residues located along the "ceiling" of the chan

192 bearing alanine substitutions for positively charged residues located at positions 5, 7, 10 and 11 ar

193 wn to be governed by a cluster of positively charged residues located in its N-terminal segment.

194 The positively charged residues located in the RNA-binding crevice play

195 Instead, we observe that substitutions of charged residues located in the TatA amphipathic helix l

196 at sequentially neutralized three positively charged residues (Lys-49, Lys-53, and Arg-57) within the

197 ter molecules in protein cavities containing charged residues may be subject to entropy changes that

198 condense on the protein ions formed via the charged residue mechanism.

199 Pentamerization forms a barrel lined with charged residues mediating pH-responsive dissociation an

200 the ion evaporation model (IEM), whereas the charged residue model (CRM) applies to large globular sp

201 For native ESI the charged residue model (CRM) is favored; it entails the l

202 Our results confirm the Rayleigh/charged residue model (CRM).

203 ow flow rates the protein molecules follow a charged residue model of ionization mechanism, and at hi

204 evaporation to dryness, as envisioned by the charged residue model.

205 ty, these results cannot be explained by the charged-residue model alone.

206 The charged-residue model is most often used to explain the

207 the coupling between conserved ion pairs and charged residues modulate the proton transfer dynamics,

208 ructure contains a large patch of positively charged residues, most of which are evolutionarily conse

209 nity block, and that substituting negatively charged residues (N171D, N171E) at this position dramati

210 main are not due solely to interactions with charged residues near phosphorylatable serines and provi

211 ee energy in the LD oil phase and positively charged residues near predicted hairpin hinges that beco

212 These peptide structures commonly positioned charged residues near the membrane interface to promote

213 zyme and directly by contributing positively charged residues near the RNA-binding cleft.

214 nteractions and is allosterically coupled to charged residues near the site of SWCNT attachment.

215 Nascent proteins enriched in negatively charged residues near their C-terminus eject the fastest

216 R24A,R25A)-cystatin C, with substitutions of charged residues not involved in enzyme inhibition, was

217 s, we identified a subset of hydrophobic and charged residues of CC MBS (localized within and adjacen

218 racts with a cluster of conserved positively charged residues of Fhc, allowing for strong interaction

219 Two negatively charged residues of KCNE3 (D54 and D55) are found essent

220 anti-aggregation agents targeting positively charged residues of proteins undergoing amyloidogenic pr

221 Positively and negatively charged residues of ribosomal proteins tend to be cluste

222 cherichia coli to re-assess the roles of the charged residues of TatA.

223 DAMGO)-related glycopeptides by altering the charged residues of the amphipathic helical address were

224 region of the N-Cap interact with negatively charged residues of the C-linker domain.

225 ibility and the reduced number of negatively charged residues of the D219/E220 deletion mutant, we me

226 atic interaction between E220 and positively charged residues of the linker in TIS11d.

227 4 PMCA is disturbed by changes of negatively charged residues of the N-terminal region.

228 bc9 residues interacting with the negatively charged residues of the NDSM.

229 ioleoyl-sn-glycero-3-phosphocholine bilayer, charged residues of the protein are trapped in the hydro

230 in place by the interactions with positively charged residues of VP1.

231 RGS19 lacks a charged residue on the alpha (4) helix that is present i

232 Positively charged residues on actin, particularly Lys(326) and Lys

233 A constellation of charged residues on and around the arginine-rich helix o

234 ke domains results in clusters of positively-charged residues on each becoming arranged to form a con

235 The charged residues on its surface made favorable electrost

236 D-1 was correlated with conserved positively charged residues on predicted amphipathic alpha-helices,

237 re we have experimentally tested the role of charged residues on stability and folding kinetics of on

238 In addition, we reveal a cluster of charged residues on the Bre1 RING domain that is critica

239 s interact in succession with the negatively charged residues on the channel protein.

240 ed a new interaction between complementarily charged residues on the cpFtsY G-domain and the vicinity

241 Mutation of several conserved and positively charged residues on the exterior surface of EcDnaB resul

242 Charged residues on the LBD surface form pathways that f

243 e high-occupancy sites in close proximity to charged residues on the protein surface.

244 the energy of insertion of TRPV1 by exposing charged residues on the S4 segment to solution.

245 g face using NMR and then mutated positively charged residues on this surface with a series of 16 Ala

246 when D231 and K178 were replaced with larger charged residues or when their positions were exchanged.

247 in an assembled state, suggesting that these charged residues play a critical role in the protein tra

248 However, two charged residues present in the modelled interface with

249 binding site, including only two positively charged residues (R122 and K141) positioned precisely in

250 The deleted fragment contains the positively charged residues R198 and K201, adjacent to layers 7 and

251 In addition, mutation of positively charged residues required for lipid binding disrupted VA

252 We identified a network of mainly charged residue-residue interactions spanning from the P

253 tin-3, but further mutagenesis of negatively charged residues revealed additional structural componen

254 nserved, the linear patterning of oppositely charged residues shows minimal variation.

255 ency toward both enzymes, whereas negatively charged residues significantly reduced it.

256 le factors that tune the pKa of its embedded charged residue, specifically its primary proton accepto

257 N-glycosylation efficiency, while positively charged residues such as Arg suppressed N-glycosylation.

258 These highly stable domains are rich in charged residues (such as Arg, Lys, and Glu) with potent

259 be achieved by neutralizing several specific charged residues suggesting that they may play an active

260 Further increasing the polarity, by adding charged residues, switches the insertion pathway to a Yi

261 while nascent chains enriched in positively charged residues tend to eject much more slowly.

262 ugar transporters, we identify an additional charged residue that may be essential for effective H(+)

263 we show that replacements of the positively-charged residues that contribute to the activity of the

264 Here we examined the role of conserved charged residues that form electrostatic networks near t

265 ystematically analyze each of the negatively charged residues that mediate binding of Ca(2+) to the b

266 itution of one of a Gly-Gly pair with highly charged residues that significantly increase structural

267 Strikingly, the addition of a positively charged residue to either the translocated region or the

268 s unclear to what extent CDRs can accumulate charged residues to increase antibody affinity without c

269 reducing the thermodynamic cost of exposing charged residues to the hydrophobic core.

270 , also showed that mutating highly conserved charged residues to the oppositely charged amino acid ha

271 Changing either of two negatively charged residues to uncharged residues eliminates the ab

272 ild-type sequence, which contains well-mixed charged residues, to increase charge segregation.

273 in a flexible loop region to the bulkier or charged residues tyrosine, histidine, and glutamic acid.

274 er salt bridges between the alphaC-helix and charged residues upstream of the NtA motif.

275 e determined by a combination of fraction of charged residues values and the linear sequence distribu

276 he linear sequence patterning of proline and charged residues vis-a-vis all other residues.

277 When this positively charged residue was introduced to either TRPV6 or TRPV5,

278 In contrast, the conserved positively charged residue was not required for the E. coli YidC or

279 an equal number of positively and negatively charged residues, we found a striking correlation T( *)c

280 athic helices of all three peptides with the charged residues well exposed to the water phase.

281 Charged residues were poorly tolerated, conferring extre

282 removes the desolvation penalty paid by the charged residue, whereas the third introduces unanticipa

283 beta2e encompassing a cluster of positively charged residues, which is strictly required for membran

284 d MMP-9 most greatly favored the presence of charged residues with preference for the Gly-Asp-Lys ser

285 a hydrophobic patch surrounded by positively charged residues, with subtle differences from other ICK

286 of the PKA-targeted serine with a negatively charged residue within the ICD of alpha3GlyRs and of chi

287 urans revealed that the conserved positively charged residue within transmembrane segment one (at pos

288 ct from DR1101 in its ability to accommodate charged residues within all but one of its binding pocke

289 essential in YtgP; YtgP possesses additional charged residues within its predicted cavity that are es

290 Segregation of oppositely charged residues within linear sequences leads to high k

291 ed substrates, we find that a combination of charged residues within the alpha repeat and charged res

292 A pair of positively charged residues within the amphipathic helix (the basic

293 Based on identified contacts, positively charged residues within the external waist region were m

294 Conversely, mutating positively charged residues within the hydrophobic region of AAP2 a

295 terized viruses with mutations engineered at charged residues within the mu1 loop formed by residues

296 segregation versus clustering of oppositely charged residues within the primary sequence.

297 ated ion channels (VGICs) contain positively charged residues within the S4 helix of the voltage-sens

298 ractions mediated by the abundant positively charged residues within these regions.

299 We further identified conserved charged residues within TMD0 transmembrane helices that

300 Phe-1086 (in NBD2) with hydrophobic but not charged residues yielded active mutants.