ライフサイエンスコーパス: chase

1 idues in protein during a 24-h pulse and 4-d chase.

2 nrichment in the pulse and a decrease in the chase.

3 ministered in neonates followed by a 1-month chase.

4 determined using (35) S radiolabeling pulse chase.

5 8-OH-DPAT also increased latencies to chase.

6 all intestinal crypt cells after a four-week chase.

7 elemental cycling via a stable isotope pulse-chase.

8 ly increased their vocalization rates during chases.

9 aches, flight loops and dives, hovering, and chases.

10 ositories, and forward and backward citation chasing.

11 ptic components would be compared with pulse-chase (15)N labeling in mice and (14)C content in human

12 ramine pretreatment, a 1.5 mg/kg desipramine chase administered 10 min after tracer injection, and sa

13 on of Persist End Atrial Fibrillation Study [CHASE-AF]; NCT01580124).

14 t are difficult for defector to discover and chase after.

15 Herein, we develop ratiometric pulse-chase amidination mass spectrometry (rPAm-MS) as a site-

16 rmine translation rates of messages by pulse-chase analyses in living Escherichia coli cells and show

17 Pulse-chase analyses reveal that active site inhibitors do not

18 Metabolic pulse-chase analyses, small molecule inhibitor treatments, and

19 Using lineage tracing and DNA pulse-chase analyses, we identified an anatomical location in

20 sing a combination of steady state and pulse-chase analyses, we show that FKBP38 knockdown increases

21 Furthermore, the primer-extension pulse-chase analysis affirmed that the reconstituted N. crassa

22 Pulse-chase analysis demonstrated that the absence of the term

23 Pulse-chase analysis indicated that in S6-expressing cells, al

24 Fluorescence pulse-chase analysis of an internally tagged Cse4 reveals that

25 Using metabolic labeling and pulse-chase analysis of HIV-1 Gag proteins, we verified that c

26 rthogonal clickable fluorophores, dual pulse-chase analysis of Lck revealed accelerated palmitate cyc

27 Pulse-chase analysis revealed that pre-termination ribosomes t

28 Pulse-chase analysis showed that the rates and amount of crypa

29 Quantitative imaging, pulse-chase analysis, and high-resolution ratiometric live-cel

30 Parabiosis, bromodoxyuridine (BrdU) pulse-chase analysis, and intranasal instillation of tracers i

31 As shown through a pulse-chase analysis, the mdVp1s were derived from the newly s

32 Moreover, using pulse-chase analysis, we show that this activation is maintain

33 se of global translation inhibitors during a chase and also provides a "built-in" reference protein.

34 om the cell surface of about 50% after a 4 h chase and became undetectable if clathrin-coated pit-med

35 As measured by pulse-chase and cycloheximide chase assays, a major binding si

36 Pulse-chase and cycloheximide-chase assays demonstrated that t

37 Pulse-chase and endoglycosidase H analysis demonstrate that th

38 Pulse-chase and kinetic spectral experiments and modeling esta

39 mploying histone and nucleotide double-pulse-chase and lineage tracing, we show that the early SC des

40 Pulse chase and mutational analysis indicated that HRD1 inhibi

41 sequencing (Bru-Seq) and bromouridine pulse-chase and sequencing (BruChase-Seq) to assess genome-wid

42 processing and stability by metabolic pulse-chase and surface On-Cell Western blots revealed that WT

43 We used EdU pulse-chase and tissue-reconstruction approaches to analyze ho

44 We used stable-isotope pulse-chase and total proteome mass-spectrometry to identify p

45 ion-ratchet model, where ParB on the plasmid chases and redistributes the ParA gradient on the nucleo

46 ereby overcoming the problem of significance chasing and approximate replications.

47 whale, including rorqual whales, show active chasing and feeding, i.e., skimming, suction, and engulf

48 This guidance law promotes tail-chasing and is not thrown off by erratic manoeuvres, mak

49 We show that females use fighting, chasing and mounting behaviors to rapidly establish high

50 ly recreated the observed interactions, i.e. chases, and best matched the observed spatial patterns f

51 We show that the HaloTag pulse-chase approach is a nontoxic alternative to inhibition o

52 re we show, by combining two different pulse-chase approaches--genetic fate-mapping with stable isoto

53 tion seeking is likely to manifest in thrill-chasing art that draws on the arousing interplay of posi

54 bly, after 8 weeks of labeling and a 12-week chase, as much as 44% of all granular neurons in the den

55 A pulse-chase assay of common SDHB missense mutations in transfe

56 Cycloheximide chase assay revealed that EMD tended to shorten the half

57 Cycloheximide chase assay showed a decrease in the half-life of KLF8 p

58 Moreover, a pulse-chase assay showed that two chimeric precursors with the

59 gradation as measured by cycloheximide (CHX) chase assay was increased in the presence of CHIP.

60 eporter assay, in vivo ubiquitination assay, chase assay, and promoter activity assay to reach the co

61 stability of KLF5 as shown by cycloheximide chase assay, indicating that SMURF2 specifically destabi

62 lycosylated F27C variants in metabolic pulse-chase assays coupled with flow cytometry and cell surfac

63 (3)H-uridine pulse-chase assays demonstrate that BLM expression is required

64 Pulse-chase and cycloheximide-chase assays demonstrated that the marked reduction in t

65 Cycloheximide chase assays revealed a shortening of APP half-life with

66 of immobilized 11-residue peptides and pulse-chase assays to examine the substrate specificity of mou

67 During import-chase assays using intact chloroplasts, Plsp1 was incorp

68 Pulse-chase assays with BrdU confirmed intracellular replicati

69 rmation and a lack of equilibration in pulse-chase assays) were also seen with (rat) P450 2B1, which

70 As measured by pulse-chase and cycloheximide chase assays, a major binding site Nedd4-2 mutant had a

71 In cycloheximide chase assays, PIAS4 slowed immature F508del degradation

72 otein stability estimated from cycloheximide chase assays; and 3) prevent the CaValpha2delta1-mediate

73 m, focusing on later aspects of transport by chasing Au-Fc before fixation.

74 led receptors (GPCRs) for chemoattractant to chase bacteria through chemotaxis and then use phagocyti

75 discoideum is a professional phagocyte that chases bacteria through chemotaxis and engulfs them as f

76 through a combination of the BrdU/EdU pulse-chase, based on the circadian pattern of DNA replication

77 d in an unbiased screen for increased female chasing behavior revealed the involvement of another sex

78 Chasing behavior, and the latencies to chase or quit, du

79 ated from wild-type animals induce male tail-chasing behavior, while ECVs isolated from klp-6 animals

80 Participants employed either chasing behaviour, in which gaze follows a target's traj

81 e analog JHC1-64 and by reversible and pulse-chase biotinylation assays showing evidence for lysosoma

82 Indeed, pulse-chase biotinylation experiments in IECs lacking AnxA2 de

83 chemoattractant GPCR that mediates not only chasing but also ingesting bacteria.

84 When chased by the addition of cold ATP, 90% of the phosphoen

85 simultaneous, opportunistic, short-distance chasing by dogs pursuing multiple prey (rather than long

86 Neutrophils were directly seen to chase C. neoformans cells and then rapidly internalize t

87 rting (33)P-phosphate-pulsed (32)P-phosphate-chased cells, we demonstrate that both Prochlorococcus a

88 Pulse-chases combined with mutagenesis studies reveal that Ser

89 wolf" display, in which one item (the wolf) chased continuously, but its goal (i.e., the sheep) freq

90 The enzyme chondroitinase (Chase) degrades inhibitory chondroitin sulfate proteogly

91 Additionally, a desipramine chase did not change the cardiac (123)I-MIBG uptake (del

92 orted to hunt with energetically costly long chase distances.

93 'PO4-end exposed by nascent RNA cleavage and chases down the RNA polymerase.

94 , in vitro binding studies utilizing a pulse-chase electrophoretic mobility shift assay protocol reve

95 using steady-state, pre-steady-state, pulse-chase, equilibrium-binding titrations, and stopped-flow

96 of n = 5 Harris' Hawks Parabuteo unicinctus chasing erratically manoeuvring artificial targets.

97 loss, we performed a bromodeoxyuridine pulse-chase experiment and found that a subset of ependymal la

98 amination of selected mutants during a pulse-chase experiment demonstrated an increase in F protein c

99 starting point for a crystallographic pulse-chase experiment of the active site during turnover.

100 We employed a carbon-13 pulse-chase experiment to investigate how a temperate estuarin

101 Here, using a (13)C pulse-chase experiment, we demonstrate how trophic structure i

102 iphosphate (mantATP) is replaced by ATP in a chase experiment.

103 Using nucleoside pulse-chase experiments and clonal analysis, we determined tha

104 EdU pulse-chase experiments and in vivo tracking of individual tra

105 The study shows that (13)CO2 pulse-chase experiments are powerful in elucidating, under in

106 Lipid turnover rates were studied by pulse-chase experiments at the single cell level.

107 Pulse-chase experiments confirm that LRAD3 expression signific

108 (14)CO2 pulse-chase experiments confirmed that water deficit enhanced

109 of tumour cell proliferation with TMZ, pulse-chase experiments demonstrate a tumour re-growth cell hi

110 Pulse-chase experiments demonstrate that the bulk population o

111 EdU pulse-chase experiments demonstrated a perivascular cancer ste

112 Western immunoblots and pulse-chase experiments demonstrated that EsaR is stable in vi

113 Pulse-chase experiments demonstrated that the down-regulation

114 (54)Mn chase experiments demonstrated that the mutant was not a

115 BrdU pulse-chase experiments demonstrated the longevity of the DEX-

116 The 5-ethynyl-2'-deoxyuridine pulse-chase experiments further reveal that this stem cell pop

117 In organello, pulse labelling and pulse-chase experiments have enabled us to track the mitochond

118 Pulse-chase experiments identified sites of cell surface growt

119 Pulse chase experiments in 293 T cells expressing rhGAA with o

120 Pulse-chase experiments in gonococci demonstrated that LtgA pr

121 However, antibiotic chase experiments in vivo indicate that FtsZ degradation

122 elemental effect using dNTPalphaS, and pulse-chase experiments indicate that a rapid phosphodiester b

123 Pulse chase experiments indicate that the newly-generated cell

124 Inducible cell line and cycloheximide-chase experiments indicate that these drugs prevent inco

125 Pulse-chase experiments indicated decreased protein turnover i

126 Optical pulse-chase experiments of Dendra2-tau demonstrate that A152T

127 hey enable regional optical marking in pulse-chase experiments on live cells and tissues, and they ar

128 occurred at 2.1 muM(-1) s(-1), and the pulse-chase experiments revealed an ATP-promoted isomerization

129 polyadenylated RNA stability via 5-EU pulse-chase experiments revealed RNAs with shorter half-lives

130 Pulse-chase experiments revealed that A774wt and avirulent rec

131 Fate mapping using BrdU pulse-chase experiments revealed that such deficits may origin

132 Furthermore, pulse-chase experiments revealed that the binding of QPD mutan

133 Biotin chase experiments revealed that the immature surface cha

134 A series of pulse-chase experiments revealed that the origin of aortic vas

135 Pulse and chase experiments show that LDAH enhances TAG biogenesis

136 ity of the aldehydes for P450 2A6, but pulse-chase experiments showed only limited exchange with adde

137 Pulse-chase experiments showed that collagen type I secretion

138 Cycloheximide chase experiments showed that Ecm7 was stabilized by Mid

139 Radioactive nucleotide chase experiments showed that the mechanism of TBSV repl

140 Here, we report genetic pulse-chase experiments that define osteoblastic cells as shor

141 ues to follow formation of NR by using pulse-chase experiments to examine protein and lipid delivery

142 n culture and bromodeoxyuridine (BrdU) pulse-chase experiments to identify and evaluate stem cell fun

143 By combining pulse-chase experiments to measure the rate at which the arres

144 Finally, pulse-chase experiments using [(14)C]serine revealed that Ups1

145 In pulse chase experiments using a deconvolved, confocal line sca

146 ocapsids into virions as determined in pulse-chase experiments was dependent on the activity of actin

147 Pulse-chase experiments with 5-EU allowed us to determine RNA

148 lags were observed in acid formation; pulse-chase experiments with carrier aldehydes showed only lim

149 ne binding event), consistent with data from chase experiments with catalytically inactive mutant Pol

150 Optical pulse-chase experiments with Dendra2-tagged aSyn versions indi

151 Bromodeoxyuridine pulse-chase experiments with short survival times suggest that

152 In pulse-chase experiments, 14-3-3beta increased the synthesis of

153 In fact, as directly demonstrated by pulse-chase experiments, EECs in the vascularized, but not in

154 In pulse-chase experiments, either miR-33 overexpression or knock

155 lecular imaging, and [(35)S]methionine pulse-chase experiments, together with lysosome (chloroquine a

156 In pulse-chase experiments, various chase periods decreased neck

157 Using pulse-chase experiments, we also discovered that the pectin ne

158 in vivo imaging and bromodeoxyuridine pulse-chase experiments, we have analyzed growth and regenerat

159 are further supported by proteome-wide pulse-chase experiments, which show that the loss of UCH37 act

160 tein stability as confirmed by cycloheximide chase experiments.

161 l approach, human islets were used for pulse-chase experiments.

162 plant Pentalinon andrieuxii by (13)CO2 pulse-chase experiments.

163 ding entity precipitations and cycloheximide chase experiments.

164 7-10 h), which we confirmed by in vivo pulse-chase experiments.

165 ility of ORE1 in vivo in MG132/cycloheximide-chase experiments.

166 rved processivity in pregnenolone/DHEA pulse-chase experiments.

167 ohistochemistry, and bromodeoxyuridine pulse-chase experiments.

168 Fluorescent pulse-chase fate-tracking documented dynamic nucleo-cytoplasmi

169 During the chase, feed was changed to an unlabeled equivalent feed

170 some insects that are used for detecting and chasing females.

171 Pulse-chase fluorescent labeling indicates that Nup188 populat

172 enzyme rapidly decayed to less than 20% when chased for 3 s by the addition of ADP.

173 In this study, gulls were chased from a Lake Michigan beach using specially traine

174 Chasing HIV-1 across the genotype landscape, unequipped

175 how coevolutionary adaptations, but instead "chase" hosts based on the herbivore's own traits at the

176 ung mice, LRCs were detectable after a 16-wk chase in adults.

177 During chase in ascorbate- free medium, anMan-containing HS dis

178 We evaluated the effects of Chase in rhesus monkeys that had undergone C7 spinal cor

179 rol-loaded cyclodextrin for 1 h, followed by chase in serum-containing medium.

180 tivity influence a behavioral analog of loss-chasing in rats.

181 ents did not retaliate as expected (i.e., by chasing) in response to increased cheating by cleaners.

182 A desipramine chase increased (11)C-hydroxyephedrine washout from the

183 c retention was sensitive to a NET inhibitor chase, indicating a cycle of continuous NET uptake and r

184 n, we administered multiple intraparenchymal Chase injections below the lesion, targeting spinal cord

185 result in predator-prey-like non-reciprocal chasing interactions.

186 he middle exon of yeast EFM5 or HMRA1 can be chased into circular RNA when the exon is sufficiently l

187 RNA-containing replication intermediates are chased into mature mtDNA, to which they are thus in prec

188 CDP-Chase is the first identified member of a novel Nudix fa

189 Finally, a pulse-chase isotope enrichment experiment was conducted by mea

190 Pulse-chase kinetic transport assays on four of the top-rankin

191 wild-type activity and single-turnover pulse-chase kinetics.

192 itoneally delivered every other day to pulse-chase label in vivo endogenous cardiac replication.

193 Using pulse-chase label retention experiments and multiplexed in sit

194 mplementary methods of genetic H2B-GFP pulse-chase labeling and BrdU incorporation in mice, we show s

195 turnover in adipocytes using metabolic pulse-chase labeling and high resolution mass spectrometry.

196 ddressed this question using metabolic pulse-chase labeling and quantitative mass spectrometry to obt

197 In the present study we use pulse-chase labeling experiments in conjunction with isolated

198 ight haloalkanes as blocking agents in pulse-chase labeling experiments with the cell-permeable tetra

199 vivo lineage tracing, and HSC-specific pulse-chase labeling have provided novel insights on B1a-cell

200 In vivo pulse-chase labeling identified galactoglycerolipid pools as a

201 Pulse-chase labeling indicates that growth is heterogeneous an

202 Improved pulse-chase labeling of endogenous interphase chromosomes yiel

203 Using in vivo pulse-chase labeling of neutrophil DNA with 6,6-(2)H2-glucose,

204 Here, we describe a metabolic pulse-chase labeling protocol using 4-thiouracil combined with

205 asomal degradation of NHK in metabolic pulse-chase labeling studies.

206 we present a genetic screen coupled to pulse-chase labeling that allow us to identify proteins select

207 acetate incorporation into MGDG during pulse-chase labeling, indicating a reduced flux through the pl

208 P-tag for studying protein turnover by pulse-chase labelling.

209 By combining pulse-chase lineage tracing and single-cell RNA sequencing, we

210 r the biomedical research community to 'stop chasing Mendel?'

211 Pulse-chase metabolic labeling reveals that the half-life of T

212 cell-surface cross-linking, FRET, and pulse-chase metabolic labeling, we demonstrate that deleting t

213 [9-11] using a reciprocal metabolomic pulse-chase method.

214 ehicle) emission levels were measured by the chasing method under real-world conditions at a depot ar

215 ct greatly, with a study employing C14 pulse-chase methodology concluding 1% annual turnover in youth

216 mino acids in cell culture (SILAC) and pulse-chase methods to generate a global quantitative map of d

217 terminal with resistance to a NET inhibitor chase, mimicking physiologic norepinephrine turnover.

218 umptions and restrictive models in the pulse-chase model artificially eliminated high-turnover soluti

219 Similarly, the pulse-chase model was acutely sensitive to assumptions of inst

220 ressed in a tetracycline (Tet)-based protein chase model, constitutive heat shock cognate 70 (Hsc70)

221 Three-station bolus-chase MR angiography with real-time fluoroscopic trackin

222 hippocampal dentate gyrus, using dual pulse-chase, multicolour gamma-retroviral tracing, transmissio

223 A predator chasing multiple prey exemplifies how goals can dynamica

224 , we employed an inducible transgenic "pulse-chase" murine model (K5Tta x TRE-H2BGFP) to localize, pu

225 Pulse-chase, Northern blotting, and primer extension analyses

226 g a combination of lineage tracing and pulse-chase of actively proliferating chondrocytes, we here de

227 nt of protein of interest half-life by pulse-chase of HaloTag ligands is not widely employed because

228 The chase of MinD by MinE creates the in vivo oscillator tha

229 ertial technology to record 1,119 high-speed chases of all members of a pack of six adult African wil

230 Chasing behavior, and the latencies to chase or quit, during sequences of unfavorable outcomes

231 swapped leader-follower roles and performed chases or coordinated manoeuvres by copying the heading

232 After a 6 d chase period, c. 26.8% of the (13) C remaining in the sy

233 Genetic labeling, followed by a chase period, showed that acinar cell replacement is not

234 C is probably modest, but may increase with chase period.

235 (ELCs) were label-retaining after a 140-day chase period.

236 exposure, as demonstrated with 2- or 4-week chase periods after labeling.

237 s available for > 24 h, indicating that long chase periods bias outcomes and fail to accurately simul

238 In pulse-chase experiments, various chase periods decreased neck cell label but did not incr

239 inorganic N and amino acid (l-alanine), with chase periods from 1 min to 24 h, to investigate the dur

240 (BrdU) in their drinking water, followed by chase periods without BrdU, or combined with intraperito

241 eling of the label-retention data during the chase phase supported the existence of a slowly cycling

242 collected at defined times during pulse and chase phases.

243 dual-reporter human cell lines and devised a chase protocol to quantify proteasomal degradation of a

244 separated glucose populations using a pulse-chase protocol.

245 oration into replicating viral DNA and pulse-chase protocols, we found that viral DNA synthesis occur

246 n, claudin strands break and reanneal; pulse-chase-pulse analysis using SNAP-tagged claudins showed p

247 Pulse-chase-pulse experiments with BrdU and EdU, and DNA conte

248 AV-infected cells and biochemically by pulse-chase radiolabeling experiments.

249 Pulse-chase radiolabeling reveals that a ypk1Delta mutant exhi

250 Using pulse-chase radiolabeling, peptide-N-glycosidase F treatment,

251 ride significantly reduced the proportion of chase responses, and the mean number of consecutive chas

252 esponses, and the mean number of consecutive chase responses, in a dose-dependent manner.

253 riods twice as long as signaled originally ('chase' responses).

254 approaching conspecific, even though it was chasing salmon, not threatening.

255 Moreover, Chase significantly increased corticospinal axon growth

256 We propose that pulse-chase SILAC labeling is a useful tool for studying rates

257 ngs demonstrate that single time point pulse-chase SILAC mass spectrometry-based proteomics (pSILAC M

258 Using a pulse-chase SILAC mass spectrometry-based proteomics approach,

259 in rats were measured in vivo using a pulse-chase stable isotope labeling experiment.

260 We report here that pulse-chase stable isotope labeling with amino acids in cell c

261 Use of high-level cooperative chase strategies (coordination and collaboration) was no

262 Using a dual pulse-chase strategy comparing palmitate and protein half-live

263 maps of protein synthesis as well as a pulse-chase strategy for determining rates of translation elon

264 We used a load-chase strategy to study the mechanisms that promote long

265 Pulse-chase studies found that CYP2E1 protein level is upregul

266 Classic pulse-chase studies have shown that actin is conveyed in slow

267 Pulse-chase studies of isotopically labeled AChE molecules sho

268 3 chk mutant/rx3 morphant fish and EdU pulse-chase studies reveal that rx3 is required to select tube

269 Cycloheximide chase studies using induced human neurons and HEK293 cel

270 In contrast, pulse-chase studies with isotope-labeled nutrients revealed im

271 Pulse-chase study showed that 14-3-3zeta siRNA decreased the a

272 A biotin pulse-chase/subcellular fractionation approach to examine ribo

273 Using a tetracycline-regulated pulse-chase system, we measured population turnover rates and

274 nvestigated in perfused rat liver by a pulse chase technique.

275 (13) C-CO(2) pulse to whole tree crowns and chased that pulse in the respiration of leaves, whole cr

276 oresis force ensues as the cargo perpetually chases the ATPase gradient, allowing the cargo to essent

277 tetracycline by binding to the ribosome and chasing the drug from its binding site.

278 ne resistance by binding to the ribosome and chasing the drug from its binding site.

279 n clinical contexts and addiction folklore ("chasing the first high"), little is known about the rele

280 In PRT assays, one measures, during a chase, the ratio of a test protein to a long-lived refer

281 The chasing-the-optimum method has been introduced for refin

282 of physicians and scientists has lost years chasing these studies, and patients have been placed at

283 Modeling loss-chasing through analogous behaviors in rats could facili

284 nd then blocked in second cytokinesis during chase to determine distribution of immortal versus newly

285 tiple short, high-speed, mostly unsuccessful chases to capture prey, while cheetahs (Acinonyx jubatus

286 Hand function improved significantly in Chase-treated monkeys relative to vehicle-injected contr

287 eome misfolding stress is applied in a pulse-chase-type experiment.

288 Ever since Hershey and Chase used phages to establish DNA as the carrier of gen

289 Using pulse-chase vital dye labeling to mark newly forming teeth in

290 ediastinum ratio, 1.99 +/- 0.12 (desipramine chase) vs. 2.05 +/- 0.16 (controls), not statistically s

291 dult mice administered (15)N-thymidine pulse-chase, we find that proliferating crypt cells dilute the

292 cetaceans and pinnipeds accomplish deep-sea chases, we deployed animal-borne instruments that record

293 ion were usable, as when predators abandon a chase when sure of the prey escaping.

294 and the Child Heart Health Study in England (CHASE), which studied 9-10 year-old children (538 Indian

295 ntially outweighs the cost of multiple short chases, which indicates that African wild dogs are more

296 lanthraniloyl)adenosine-5'-O-diphosphate and chased with ADP, the SRX is not seen, indicating that tr

297 hioacetimidate (SMTA) for pulse time, t, and chased with excess of S-methyl thiopropionimidate (SMTP)

298 he right pSTS, compared with (1) a wolf that chases with a single unchanging intention, (2) very simi

299 Here, we combine H2B-GFP-based pulse-chasing with cell-surface markers to distinguish quiesce

300 s to increase NEUROG3 expression and further chased without inhibitors.