ライフサイエンスコーパス: chemically-defined

1 media, attachment surfaces and splitting are chemically defined.

2 Glucan phosphate, a water-soluble, chemically defined (1-->3)-beta-D-glucan biologic respon

3 Here, to study them, we developed a chemically defined 3D model where conventional pluripote

4 ains three breakthrough articles: AntiBodies Chemically Defined (ABCD) curates antibody sequences and

5 ost-implantation mouse and rat embryos using chemically defined, activin-containing culture medium th

6 o allows high-yield (>250%) MN production in chemically defined adherent cultures.

7 e that these materials may also be useful as chemically defined adjuvants.

8 ified histones were used to generate various chemically defined ADP-ribosylated chromatin substrates,

9 V often received excitatory inputs from two chemically defined afferent pathways.

10 yclodextrin represents the first transparent chemically defined agar and the first urease indicator a

11 We now show that the addition of a chemically defined AGE (methyl-glyoxal-BSA) to low-AGE m

12 These chemically defined alternatives to currently used, undef

13 The ability to generate chemically defined analogues of GPI-anchored proteins is

14 PRosettaC, on the other hand, leverages chemically defined anchor points to yield more geometric

15 Furthermore, the observed channels are chemically defined and have diameters related to oligome

16 potent stem cells (GM23338) with a series of chemically defined and serum-free media to induce format

17 m self-renewal of hES cells under completely chemically defined and xeno-free conditions without ROCK

18 This chemically defined and xenogen-free method enables repro

19 Here, we report a feeder-free, scalable, chemically defined, and modular alveolosphere culture sy

20 oduction of organoids and the development of chemically defined, animal origin-free (CD-AOF) media an

21 A chemically defined anti-CXCR4-auristatin antibody-drug c

22 These experiments support the notion that chemically defined antibody conjugates can be synthesize

23 The resulting chemically defined antibody-drug conjugates represent th

24 These chemically defined antigens identified the M antigenic d

25 mer-based therapeutics and suggest that such chemically defined APCs may afford a useful targeted del

26 blished connections between glutens and CeD, chemically defined, bioavailable peptides produced from

27 We demonstrate that H. pylori grows in the chemically defined broth medium Ham's F-12 nutrient mixt

28 tups, including particles formed in vitro in chemically defined buffers or Xenopus egg extracts and c

29 Chemically defined (CD) and hydrolysate-supplemented med

30 Chemically-defined (CD) media offer critical advantages

31 Thus, we used a chemically defined cell culture medium based on the meta

32 proven important for producing homogeneous, chemically defined complexes for structural studies.

33 specific infectivity have been produced from chemically defined components in vitro.

34 The chemically defined components of the bacterial envelope

35 CM) to a non-covalent structure with minimal chemically defined components represents an attractive a

36 Using sulfated versus non-sulfated forms of chemically defined compounds in binding assays employing

37 primitive neuroepithelium within 1 wk under chemically defined condition.

38 an undifferentiated, pluripotent state under chemically defined conditions in the absence of feeder c

39 ing this critical reproductive process under chemically defined conditions in vitro.

40 Using hiPSCs derived under chemically defined conditions on synthetic matrices, we

41 neural progenitor cells (NPCs) maintained in chemically defined conditions to study HCMV replication

42 oculture with other cell types and relies on chemically defined conditions up to and including the ge

43 ach for the large-scale generation of MKs in chemically defined conditions using a forward programmin

44 Under chemically defined conditions, the galactofuranosyltrans

45 ted extensively for more than 18 passages in chemically defined conditions, with 10(5) starting fibro

46 Cellular reprogramming using chemically defined conditions, without genetic manipulat

47 level of 3% (previously termed hypoxia) and chemically defined conditions.

48 etic progenitors (HPs) derived from hPSCs in chemically defined conditions.

49 e a novel 3-step differentiation protocol in chemically defined conditions.

50 d populations of preOLs and mature OLs under chemically defined conditions.

51 observed in retinal explants cultured under chemically defined conditions.

52 ed serum-free cultures and recently reported chemically defined conditions.

53 o distinct populations of SMC subtypes under chemically defined conditions.

54 d from mouse embryonic stem (ES) cells under chemically defined conditions.

55 thod suitable for the routine preparation of chemically defined conjugates for in vivo applications.

56 cells (ESCs) or induced PSCs (iPSCs), under chemically defined culture conditions, with ex vivo cult

57 (hLLCs) or adrenal-like cells (hALCs) using chemically defined culture conditions.

58 the development of a long-term feeder-free, chemically defined culture system for distal lung progen

59 The chemically defined culture system for hTSC differentiati

60 Here we present a chemically defined culture system for STB and EVT differ

61 In chemically-defined culture conditions, UB organoids gene

62 Consequently, we generate a minimal chemically-defined culture medium in which IGF1 together

63 We demonstrate that the chemically-defined cysteine-engineered antibody-tubulysi

64 Here, we use chemically defined "designer" nucleosomes to investigate

65 into proteins and had the same lifespan on a chemically defined diet with or without selenium supplem

66 Using chemically defined diets, we found that association with

67 larvae were reared on one of a series of 20 chemically-defined diets ranging in their protein: carbo

68 In this report, a chemically-defined differentiation regimen and a monolay

69 Previous studies indicated that a chemically-defined, differentiation medium (DM) induces

70 We have synthesized a chemically defined, dimeric form of an erythropoietin mi

71 The chemically defined disulfide connectivity of the recombi

72 We devised a reconstituted system in which chemically defined donor membranes function to transfer

73 TA-based passaging procedure to be used with chemically defined E8 medium that serves as a tool for b

74 We report a chemically defined, efficient, scalable and reproducible

75 intravenous TPN, partially preserved with a chemically defined enteral diet, and completely preserve

76 We systemically exposed organoid samples to chemically defined "enviromimetic" compounds to examine

77 Our chemically defined expansion culture system will help to

78 We evaluated the effect of chemically defined extracts from Echinacea angustifolia

79 A chemically defined food (CDF) permits the systematic eva

80 uded organic acids, observed particularly in chemically defined formulations, and furans, pyridines,

81 owth in human saliva compared to growth in a chemically defined glucose-containing medium or a nutrie

82 ese interactions rely on the availability of chemically defined glycoconjugates that can be selective

83 uated the addition of these two nutrients to chemically defined grape juice and filter clarified Char

84 to synthesize arginine was assessed using a chemically defined growth medium.

85 s from pluripotent stem cells (PSCs) using a chemically defined, growth factor-rich medium.

86 Here we demonstrate that chemically defined guanidine 5'-diphosphate-beta-l-fucos

87 ynthesizing molecular wires designed to have chemically defined hemilabile contacts based on (methylt

88 Hepatocytes were grown in chemically defined hepatocyte growth medium (HGM) contai

89 There is currently no complete chemically defined (holidic) diet available for Drosophi

90 rolysis combined with the facile assembly of chemically defined homo- and heterodimers constitutes an

91 terest and the subsequent preparation of the chemically defined, homogeneous antibody conjugate can b

92 as a new, user-friendly route for supplying chemically defined human glycans simply by combining bio

93 using a novel rapid protocol which uses only chemically-defined human-derived media additives and sub

94 emethylation, we subjected mouse ES cells to chemically defined hypomethylating culture conditions.

95 This approach based on fully synthetic chemically defined immunogens should be generally useful

96 Using SNARE and Sly1 mutants and chemically defined in vitro assays, we separate and asse

97 s critical need, we developed a reproducible chemically defined in vitro methodology for generating r

98 hPSCs, an iNPC expansion phase with a unique chemically defined iNPC expansion medium called 'hNPSR-v

99 activates DCs through TLR11 and is the first chemically defined ligand for this TLR.

100 The solutions were chemically defined lipid concentrate (CDLC) and model bi

101 supplements (Cholesterol Lipid Concentrate, Chemically Defined Lipid Concentrate, Lipid Mixture 1, G

102 m large ( approximately 300 nm in diameter), chemically defined liposomes incubated with coatomer, Ar

103 ut-PE) was synthesized and incorporated into chemically defined liposomes to provide binding sites fo

104 Thus, the incorporation of chemically defined LN-511-E8 into biosynthetic scaffolds

105 We believe that chemically defined LNP formulations optimized for RNP-en

106 eisseria meningitidis strains that expresses chemically defined LOS components to determine the struc

107 hanol-fed rats recognized mainly a specific, chemically defined MAA epitope.

108 cost, robust, scalable culture methods using chemically defined materials need to be developed to add

109 e methods that are robust, scalable, and use chemically defined materials.

110 Here we show that a chemically defined maturation medium supplemented with t

111 dium formulations that enabled efficient and chemically defined maturation of functional CD8alphabeta

112 he growth of the Deltasse strain in rich and chemically defined media and nonimmune human blood and s

113 -inhibitory concentration of daptomycin in a chemically defined media containing L-[e-(15)N]Lys.

114 The data also suggest that chemically defined media described herein could yield th

115 onicus were surveyed by employing culture in chemically defined media devoid of peptide nutrients and

116 ty of 23 representative gut microbes using a chemically defined media lacking glucose supplemented wi

117 re matrices such as biomimetic scaffolds and chemically defined media supplemented with essential nut

118 sing substrates of known matrix proteins and chemically defined media supplemented with exogenous gro

119 The approach uses chemically defined media under feeder-free conditions, a

120 ined media using fetal bovine serum (FBS) or chemically defined media utilizing specific growth facto

121 bilities of M1 and M4 Mga mutants to grow in chemically defined media with a single sugar source comp

122 of equibactin synthesis, supplementation of chemically defined media with salicylate was required fo

123 ollagen gel cylinders that were incubated in chemically defined media with stromal-derived factor-1al

124 ost pronounced when S. aureus is cultured in chemically defined media with thiosulfate (TS) as a sole

125 ith the advent of new initiatives to develop chemically defined media, cell culture scientists screen

126 Colonies grown on two chemically defined media, including the one in which BMP

127 elf-renewing neural crest-like stem cells in chemically defined media.

128 cultivation and recovery of H. pylori using chemically defined media.

129 gated in serum (2-3 h) and differentiated in chemically defined medium (3-4 h).

130 tococcal proteases were recovered from spent chemically defined medium (CDM) and fractionated by ammo

131 Chemically defined medium (CDM) conditions for controlli

132 evelopment of competence was examined in the chemically defined medium (CDM) described by van de Rijn

133 ters were upregulated in vitro when grown in chemically defined medium (CDM) lacking BCAA.

134 The mtaR mutant grew poorly in chemically defined medium (CDM) prepared with methionine

135 cally, when aerobically grown in a low-iron, chemically defined medium (CDM), L. pneumophila secretes

136 was used to develop in silico and in vitro a chemically defined medium (CDM), which was validated exp

137 murine soft tissue and in nutrient-limiting chemically defined medium (CDM).

138 40 to 70% less CAS reactivity in deferrated chemically defined medium (CDM).

139 or alpha (TGFalpha) in the presence of a new chemically defined medium (HGM).

140 e sequence TVKHRPDALHPQ or LTTAPKLPKVTR in a chemically defined medium (mTeSR), they expressed marker

141 ar matrix coating, and either conditioned or chemically defined medium (mTeSR).

142 -Hewitt supplemented with excess glucose and chemically defined medium allowed survival for less than

143 ed in dimethyl sulfoxide (DMSO)-supplemented chemically defined medium are an excellent model system

144 When grown in chemically defined medium at an alkaline pH, strain 86-0

145 ively) were grown in continuous culture in a chemically defined medium at fast mass doubling time (t(

146 Oral viridans group streptococci, growing in chemically defined medium at pH 6.8, released HlpA into

147 ila was reduced approximately in half in the chemically defined medium compared with the same medium

148 , introducing two novel formulations: LM7, a chemically defined medium comprising seven essential com

149 ed cardiac differentiation strategy, using a chemically defined medium consisting of just three compo

150 s phenotypic abnormalities whereas growth in chemically defined medium containing 5 mM KCl more close

151 dc25A-rescued cells could not proliferate in chemically defined medium containing CSF-1 and continued

152 ts in decreased growth of H. influenzae in a chemically defined medium containing Neu5Ac, supporting

153 and the mesendoderm reporter MIXL1-GFP in a chemically defined medium containing the canonical WNT s

154 often observed when microbes are grown in a chemically defined medium containing two sugars (for exa

155 ious data showed that H. pylori grows in the chemically defined medium F-12, but not in other tissue

156 We also show that reprogramming using chemically defined medium favors formation of fully repr

157 . pylori strains were cultured in Ham's F-12 chemically defined medium in the presence or absence of

158 opper, iron, and zinc to the DMSO-containing chemically defined medium induced DNA synthesis and cell

159 grown in continuous culture with a modified chemically defined medium is regulated by growth rate.

160 Halobacterium synthesized cobalamin in a chemically defined medium lacking corrinoid precursors.

161 roduction was significantly decreased when a chemically defined medium lacking N-acetylneuraminic aci

162 give rise to cell lines that proliferate in chemically defined medium lacking serum.

163 nstrated that hemoglobin supplemented into a chemically defined medium significantly and specifically

164 n adipogenesis induction medium, but also in chemically defined medium specifically for osteogenesis,

165 gue profiling after bacterial cultivation in chemically defined medium supplemented with [U-(13)C(6)]

166 We previously described the use of a chemically defined medium supplemented with epidermal gr

167 genes in an S. mutans PTS mutant grown in a chemically defined medium supplemented with glucose.

168 Pasteurella multocida was grown in iron-free chemically defined medium supplemented with hemoglobin,

169 nhancer/promoter, we found that a serum-free chemically defined medium supports formation of embryoid

170 Because RPMI is a more stable and chemically defined medium than M3, the determination at

171 biological role for these proteins, a simple chemically defined medium that supports growth of adult

172 A chemically defined medium that supports the growth of bo

173 Also, we tested completely chemically defined medium that, when used together with

174 well in complex medium, but did not grow in chemically defined medium unless supplemented with the m

175 ains were assessed by monitoring growth in a chemically defined medium where the only source of the e

176 oderm and surface ectoderm, in a serum-free, chemically defined medium with 10-50 ng/ml of FGF-2 indu

177 tential by culturing them as aggregates in a chemically defined medium with TGFbeta1.

178 ically resected patient tumor tissue using a chemically defined medium without cell dissociation.

179 rs with a predominantly dorsal identity in a chemically defined medium without known morphogens.

180 cells are continuously grown in a completely chemically defined medium without serum supplementation,

181 term dimethylsulfoxide (DMSO) culture, fed a chemically defined medium, are highly differentiated and

182 In a chemically defined medium, exogenous CSP does not induce

183 ells were co-cultured in vitro in serum-free chemically defined medium, functional anchoring junction

184 In a chemically defined medium, growth of the wild-type strai

185 The fucose phenotype, shown in chemically defined medium, is strain specific and linked

186 After growth in a chemically defined medium, stage I (lyophilized) culture

187 When control hESCs were grown in chemically defined medium, their expression of markers o

188 of H2O2 and failed to grow aerobically in a chemically defined medium, unless supplemented with acet

189 Using iron-deficient, chemically defined medium, we determined that H. pylori

190 Using a chemically defined medium, we found that (p)ppGpp accumu

191 , and TGFbeta pathway activity in a minimal, chemically defined medium, we show parallel, robust, and

192 nts and cultured as monolayers in serum-free chemically defined medium.

193 either a Casitone-based complex medium or a chemically defined medium.

194 re BCKAD enzyme activity than cells from the chemically defined medium.

195 or even survive--when plated in mitogen-free chemically defined medium.

196 beta1, TGFbeta2 or TGFbeta3 was added to the chemically defined medium.

197 y role to BrnQ1 during S. aureus growth in a chemically defined medium.

198 ophic for Arg, Gln/Glu, His, Leu, and Trp in chemically defined medium.

199 The observed effects were more striking in a chemically defined medium.

200 ne stifle joints were cultured in serum-free chemically defined medium.

201 nd expansion of the initial SC population in chemically defined medium; (3) a magnetic-activated cell

202 on a rat-tail collagen coated dish and fed a chemically-defined medium supplemented with 2% dimethyls

203 T-II binds to the ESCRT-III subunit Vps20 on chemically defined membranes in a curvature-dependent ma

204 Here, we describe a chemically defined method for generating epicardium and

205 Growth of H. capsulatum mycelia in chemically defined minimal medium produced pigmented con

206 Growth of H. capsulatum yeast in chemically defined minimal medium with L-3,4-dihydroxyph

207 This is the first chemically defined model of the physiological interactio

208 for reduction in cobalt binding by albumin, chemically defined model systems consisting of bovine se

209 tems, we describe a process for synthesizing chemically defined molecular constructs that likewise ac

210 tudy, we describe the synthesis and study of chemically defined mucin analogues bearing native glycan

211 ovide novel, to our knowledge, evidence of a chemically defined, multicomponent lipid system that cou

212 ction by synthetic means, the development of chemically defined multivalent ligands of effectors is s

213 We describe here a chemically defined natural N-linked glycan scaffold that

214 The retrotrapezoid nucleus (RTN) contains chemically defined neurons (ccRTN neurons) that provide

215 In addition, utilizing a chemically defined nonasaccharide acceptor, azidoethyl 6

216 ture and thymine DNA glycosylase (TDG) using chemically defined nucleosome arrays containing site-spe

217 Using chemically defined nucleosome arrays, we demonstrate tha

218 Reconstitution of our uH2B into chemically defined nucleosomes, followed by biochemical

219 eral strategy for engineering the display of chemically defined oligosaccharides on cell surfaces tha

220 gregated and formed osteoclast-like cells in chemically defined osteoclastogenesis medium with 20 ng/

221 This chemically defined platform for cardiac specification of

222 PSC)-derived neuronal cells were cultured on chemically-defined poly(ethylene glycol) (PEG) hydrogels

223 oglia/macrophage precursors were combined on chemically defined polyethylene glycol hydrogels and cul

224 Chemically defined polymers can remediate technological,

225 xic soil reactors amended with and without a chemically defined polyphenol to test this hypothesis, e

226 Triptolide (TPT) is a chemically defined, potent immunosuppressive compound is

227 ting inflammation, incubating the cells in a chemically defined protein-free medium that provided a s

228 Here we describe a chemically defined protocol for robust single-cell cloni

229 Here we describe a chemically defined protocol in which hPSCs were initiall

230 We thus developed a robust chemically defined protocol that generates CFs from huma

231 RNA-sequencing atlas and to develop a robust chemically defined protocol to derive cardiac fibroblast

232 Therefore, a chemically defined PRR agonist like poly ICLC can be a r

233 that are low-cost, robust, scalable and use chemically defined raw materials.

234 into complexes for fusion can be studied in chemically defined reactions.

235 Using a chemically defined reconstitution system, we performed a

236 Employing genetic analyses and chemically defined reconstitutions of ER-Golgi fusion, w

237 lotting (immunoblotting) in cells grown in a chemically defined rich medium or in a minimal medium.

238 (hES) cells have been developed, but finding chemically defined, robust substrata has proven difficul

239 ne glycan presentation and multivalency from chemically defined scaffolds.

240 esired biological activities, and provides a chemically defined, scalable and single cell passaging c

241 Here, we developed a chemically defined, scalable, and reproducible specifica

242 e a mechanism of sensory integration for two chemically defined sensory inputs and may have implicati

243 ion, followed by a 7-day culture period in a chemically defined serum-free basal medium (BM), with or

244 man oral mucosal keratinocytes cultured in a chemically defined serum-free culture system, devoid of

245 or 48 hr in 5% FCS-containing medium or in a chemically defined serum-free medium (ITS).

246 ssessed in cultured granule neurons grown in chemically defined, serum-free medium containing either

247 Here, we establish a chemically defined, serum-free protocol for prolonged cu

248 Using a novel, pluripotent stem cell-based, chemically-defined, serum and feeder cell-free culture s

249 an easily scalable 3D PHH spheroid system in chemically-defined, serum-free conditions.

250 Based on the fusion properties of chemically defined SNARE- proteoliposomes, it has been p

251 A series of novel chemically defined soluble oligomers of the human MHC cl

252 luable in studies aimed at the evaluation of chemically defined structures for toxicity versus immuno

253 splantation of the sensory epithelium onto a chemically defined substrate.

254 minating the need for MEF feeders by using a chemically-defined substrate.

255 Chemically defined substrates for PglC, PglJ, and PglB a

256 s in purifying WaaL homologues and obtaining chemically defined substrates.

257 bunit assembly and coated vesicle budding on chemically defined synthetic liposomes.

258 Therefore, chemically-defined synthetic hydrogels offer benefits ov

259 oglia from human pluripotent stem cells in a chemically defined system.

260 induced pluripotent stem cells (iPSCs) in a chemically defined system.

261 delta) both on unmodified templates and past chemically defined template base lesions (translesion sy

262 NA polymerase delta (pol delta) past several chemically defined template lesions including model abas

263 we report the identification of a family of chemically defined thermoresponsive synthetic hydrogels

264 TFL iron was similar to chemically defined thioferrates; both consisted of Fe(II

265 e a simple protocol that is xenogen free and chemically defined to differentiate human embryonic stem

266 In this paper we show that feeding chemically defined total parenteral nutrition (TPN) to g

267 the degradation kinetics of substrates with chemically defined ubiquitin configurations.

268 GM2-KLH/QS-21 (GMK) is a chemically defined vaccine that is one of the best devel

269 This strategy results in a chemically defined vaccine while maintaining both the st

270 ycolate (3'-PG)] was examined using a novel, chemically defined vector substrate containing a single,

271 ase activity of pure, recombinant GP12 using chemically defined wall teichoic acid analogs.

272 Establishment of chemically defined xeno-free conditions is of great inte

273 These chemically defined xeno-free substrates generate more th

274 (PAS) that support self-renewal of hESCs in chemically defined, xeno-free medium.

275 of expanding and differentiating hiPSCs on a chemically defined, xeno-free synthetic peptide substrat

276 Here we develop the first chemically defined, xeno-free, feeder-free synthetic sub