ライフサイエンスコーパス: chemostat

1 growth on glucose, lactose, and maltose in a chemostat.

2 s extinction of the bacterial culture in the chemostat.

3 rameters of plasmid-bearing cells growing in chemostat.

4 under hemin excess conditions (pH 7.0) in a chemostat.

5 f bacterial growth parameters in a simulated chemostat.

6 ypes (differing in viral resistance) in each chemostat.

7 f Escherichia coli growing in a microfluidic chemostat.

8 r mutant that outgrew its predecessor in the chemostat.

9 present in some initial concentration in the chemostat.

10 the transcriptome of NO-exposed E. coli in a chemostat.

11 500 generations of growth in glucose-limited chemostats.

12 experimental evolution in glutamine-limited chemostats.

13 tained in nutrient-limiting conditions using chemostats.

14 s, ARS, or all elements in glutamine-limited chemostats.

15 eneration for GAP1 CNVs in glutamine-limited chemostats.

16 mixed and continually fed into 10 identical chemostats.

17 e in monocultures evolving in lysine-limited chemostats.

18 ted against experimental data obtained using chemostats.

19 ive and methanogenic coculture conditions in chemostats.

20 nchronized cultures growing exponentially in chemostats.

21 Microfluidic chemostats allowed observation of intrinsic cell dynamic

22 r community consequences; coevolution in the chemostat altered the sensitivity of Synechoccocus to a

23 retic analysis and protein identification in chemostat and continuous-culture biofilm-grown populatio

24 this hypothesis, we grew diverse strains in chemostat and measured DNA replication and oxygen consum

25 s many environments, bridges the gap between chemostat and serial dilution systems.

26 smid pBR322 depended on the dilution rate in chemostat and was higher at low dilutions; (ii) high lev

27 d on Prochlorococcus MED4 grown in P-limited chemostats and batch cultures.

28 mework to model microbial transformations in chemostats and retentostats under transient or quasi-ste

29 a number of steady and unsteady continuous (chemostat) and batch culture laboratory experiments.

30 igh and low growth rates in a carbon-limited chemostat, and transcriptomic analysis was performed to

31 Blinded specimen sets from human stool, chemostats, and artificial microbial communities were se

32 er hour, were obtained from cellobiose-grown chemostats, and it was shown that one ATP is required pe

33 chemostat, indicating that the cells in the chemostat are "poor, not starving." Similar comparisons

34 GBS type V, an emergent serotype, grown in a chemostat at a cell mass-doubling time (t(d)) of 1.8 h w

35 Each species was grown in a chemostat at two different growth rates.

36 ith noise measurements of cells growing in a chemostat at well-defined growth rates, suggests that ce

37 )) to epsilon(C) = -4.1 +/- 0.2 per mille in chemostats at 90 mug L(-1).

38 calis, we used microtiter plate assays and a chemostat-based biofilm fermentor assay to examine biofi

39 fically, in this paper, we develop a simple, chemostat-based model illustrating how a process analogo

40 Utilizing chemostat-based resource-competition models, we exhibit

41 Therefore, the chemostat bears the imprint of earlier, simultaneous sti

42 In this scenario, simulations require chemostats capable of preserving the chemical concentrat

43 general and stress-specific responses under chemostat conditions in which specific growth rate-depen

44 sion is derepressed in all nitrogen-limiting chemostat conditions regardless of nitrogen source, and

45 raints of constant total population size and chemostat conditions.

46 Although in both cases the cells in the chemostat consumed most of the glucose, in neither case

47 s growing at steady state in glucose-limited chemostats corresponds most closely with the state of ce

48 low glucose concentrations under continuous chemostat cultivation with the aim to identify novel fac

49 s of S. aureus growing at different rates in chemostat culture ( approximately 6 h, 1 day and 2 week

50 s to a sudden drop in oxygen availability in chemostat culture and studied the transcriptional respon

51 ical manipulation of S. mutans in continuous chemostat culture demonstrated that steadystate levels o

52 E. coli was grown in chemostat culture so that cellular metabolism could be h

53 vels of either nitrate or nitrite, anaerobic chemostat culture techniques were employed using nrfA-la

54 igh levels of nitrate, nitrite, and formate, chemostat culture techniques were employed with fdnG-lac

55 The stochastic IBM model fails the steady chemostat culture test, because it produces excessive nu

56 tion in batch culture to P-limited growth in chemostat culture.

57 f environmental fitness than either batch or chemostat culture.

58 Utilizing chemostat-cultured human fecal bacteria, we identified t

59 microbiota were further validated using gut chemostat cultures and microbiota transfer into germ-fre

60 ply to excess gaseous IC supply, N. europaea chemostat cultures demonstrated an acclimation period th

61 n of a modest oxidative stress response, the chemostat cultures did not exhibit the massive environme

62 Chemostat cultures enabled a study of AI-2 regulation th

63 data from genes in Saccharomyces cerevisiae chemostat cultures exhibiting energy metabolic oscillati

64 Glycerol-limited chemostat cultures in defined medium of Escherichia coli

65 Four of 13 chemostat cultures monomorphic for the lac operon retain

66 ns by means of (13)C-labeling experiments in chemostat cultures of a wild-type strain, DeltacreB and

67 lization of fumarate as an electron donor in chemostat cultures of Geobacter sulfurreducens, despite

68 A novel aspect of our work is the use of chemostat cultures of M. tuberculosis which allowed us t

69 showed that indene oxidation in steady state chemostat cultures proceeds primarily through a monooxyg

70 We used steady-state oxic and anoxic chemostat cultures to demonstrate that the switch from a

71 communication, was studied in E. coli W3110 chemostat cultures using a Vibrio harveyi AI-2 reporter

72 to PQH2 extraction, cells from steady-state chemostat cultures were exposed to a wide range of physi

73 embly in Escherichia coli using steady-state chemostat cultures where we could precisely control the

74 two different parameters in nutrient-limited chemostat cultures, biomass concentration and the ratio

75 +/- 0.06 was calculated for cellulose-grown chemostat cultures, corresponding to n = 4.20 +/- 0.46.

76 Using minimal-medium batch and chemostat cultures, we comprehensively characterized spe

77 ate using carbon (serine)-limited continuous chemostat cultures.

78 imental data obtained from highly controlled chemostat cultures.

79 myces cerevisiae (yeast) in nitrogen-limited chemostat cultures.

80 diverse nutrients in batch and steady-state (chemostat) cultures of S. cerevisiae.

81 lucose limitation in batch and steady-state (chemostat) cultures of Saccharomyces cerevisiae by follo

82 from epsilon(C) = -1.0 +/- 0.5 per mille in chemostats (D = 0.090 h(-1), 88 mg L(-1)) and epsilon(C)

83 athione (GSNO) in both aerobic and anaerobic chemostats, demonstrated the expression of nitric oxide

84 phenotypes such as competitive fitness in a chemostat, DNA repair proficiency, and synthetic genetic

85 nclude the toxin/antitoxin interactions in a chemostat does not alter the qualitative results that su

86 l in which the resource exhibits logistic or chemostat dynamics and consumers have saturating (Type I

87 a defined selection is continuous culturing: chemostats enable the study of adaptive evolution in con

88 replicate experiments are adaptations to the chemostat environment and are not specific to one or the

89 ontrol amensal bacteriocin interactions in a chemostat environment.

90 meters measured in a range of community-like chemostat environments, prediction quantitatively agreed

91 robes growing on mixtures of substrates in a chemostat exhibit different substrate utilization patter

92 e the practicality of the code by modeling a chemostat experiment of atrazine degradation at low dilu

93 We conducted a long-term chemostat experiment where the marine cyanobacterium Syn

94 In a 6-mo-long replicated chemostat experiment, Synechococcus sp. WH7803 and the v

95 We first compare our model to a classical chemostat experiment, validating our proposed dynamics a

96 Chemostat experiments showed a metabolic shift induced i

97 Here, we use phosphate-limited chemostat experiments to show that acidification enhance

98 Isotope analysis of atrazine in chemostat experiments with whole cells revealed a drasti

99 unique deletions among 42 evolvants from 23 chemostat experiments.

100 In chemostats, fatty acids from glycerol-respiring cells we

101 ubstrate transport, mol ATP/mol hexose) from chemostats fed beta-glucans with degree of polymerizatio

102 gastrointestinal model comprising sequential chemostat fermentation steps that simulate digestive con

103 During chemostat fermentation that simulates colonic digestion,

104 hocyanins were detected in low amounts after chemostat fermentation.

105 effect of generation time (as controlled by chemostat flow rate) and temporal variability in nutrien

106 competition experiments in nutrient-limited chemostats followed by high-throughput sequencing of str

107 iocatalytic systems comprising the uses of a chemostat for strain improvement and radioisotopic trace

108 continuous aerobic growth in glucose-limited chemostats for more than 250 generations.

109 Competition in chemostats for two substitutable resources, methylgalact

110 e-controlled cultures using nitrogen-limited chemostats, gene expression programs are strikingly simi

111 tate cultures of Saccharomyces cerevisiae in chemostats growing on limiting galactose to two differen

112 yield analysis and growth rates derived from chemostat-grown cells (under three conditions).

113 Chemostat-grown cells, subjected to increasing carbon st

114 The intracellular concentration of DMSP in chemostat-grown S. pomeroyi DSS-3 was 70 mM.

115 es to peroxynitrite under tightly controlled chemostat growth conditions.

116 First, using recently published data on chemostat growth of Saccharomyces cerevisae under differ

117 Batch kinetic tests with chemostat harvested cells showed the maximum rate (k(max

118 aminant degradation by suspended bacteria in chemostats has shown that isotope fractionation decrease

119 sentially the same pattern was found between chemostats having a fivefold difference in steady-state

120 g prolonged culturing in L-glutamine-limited chemostats in a manner analogous to the selection of onc

121 were cultivated under high-DIC conditions in chemostats in growth medium with low concentrations of D

122 Strains isolated from three of four chemostats in which the lac polymorphism was preserved h

123 ted in batch cultures is not observed in the chemostat, indicating that the cells in the chemostat ar

124 Two chemostats inoculated with the Point Mugu culture, which

125 When the growth of bacteria in a chemostat is controlled by limiting the supply of a sing

126 ime, followed by a time gap during which the chemostat is not stimulated at all, and if the chemostat

127 When a chemostat is perturbed from its steady state, it display

128 We show that, if the chemostat is stimulated by both growth factors for a cer

129 emostat is not stimulated at all, and if the chemostat is then stimulated again by only one of the gr

130 he biomolecular level, these algae inhabit a chemostat-like environment and is consistent with the ne

131 enrichment, providing evidence that SDB is a chemostat-like environment where circulation, temperatur

132 batch, the serial dilution dynamics mimic a chemostat-like steady state.

133 A chemostat limited by a single growth-limiting substrate

134 In chemostat-maintained perpetual cultures, the proportion

135 e and the biofilm community in mixed-culture chemostat MFCs.

136 We then remeasured strain phenotypes in chemostats mimicking the metabolite-limited community en

137 een the transcriptomic profile of BCG in the chemostat model and the response of M. tuberculosis to g

138 of M. tuberculosis and also the value of the chemostat model for deconstructing components of the in

139 ycobacterium tuberculosis in vivo, we used a chemostat model to study a single aspect of the organism

140 nt taxa were further maintained ex vivo in a chemostat model.

141 models: a standard Wright-Fisher model and a chemostat model.

142 rsus extinction only under certain ranges of chemostat operating parameters.

143 he morbidostat can additionally be used as a chemostat or a turbidostat.

144 me-course experiments that were performed in chemostats or batch cultures under a spectrum of environ

145 ith spent medium supernatants from batch and chemostat planktonic and biofilms generated in continual

146 The control chemostat (PM-5L, no sulfate), achieved pseudo-steady-st

147 Sulfate (1 mM) was introduced to the second chemostat, PM-2L.

148 mum nitrification rates, consistent with our chemostat results.

149 roline, to cells growing in nitrogen-limited chemostats results in rapid, dose-dependent repression o

150 on the flow rate and feed concentration, the chemostat settles into a steady state or executes sustai

151 tograms in RGB channels were extracted using Chemostat software.

152 Results are presented from a chemostat study where the reductive dehalogenation of PC

153 level of GAP1 expression in nitrogen-limited chemostats suggests that the frequency of GAP1(circle) a

154 (gas phase, 200 ml of 5% CO2 in air x min-1) chemostat system were then investigated.

155 stems of protists in continuous experimental chemostat systems and corresponding continuous-time mode

156 lists on each galactoside were isolated from chemostats that maintained the fhuA polymorphism, whethe

157 In the other five chemostats the fhuA polymorphism, and consequently the l

158 In four out of nine chemostats the lac polymorphism persisted for 400-600 ge

159 Extending the chemostat theory to include serial-dilution cultures, we

160 fects a lake microbial community by exposing chemostats to a tau gradient spanning seven orders of ma

161 ) mutant was cultured in an NH(4)(+)-limited chemostat, two sequential spontaneous mutations occurred

162 o fully aerobic metabolism of glucose in the chemostat under conditions of glucose scarcity, homeosta

163 cultivation of Arthrobacter aurescens TC1 in chemostat under four different dilution rates leading to

164 Moreover, GBS grown in a chemostat under highly invasive conditions (t(d) of 1.8

165 Desulfovibrio alaskensis strain G20 grown in chemostats under respiratory and syntrophic conditions w

166 tinuous oxygen depletion were performed in a chemostat using nitrate as the terminal electron accepto

167 studied at four levels of iron limitation in chemostats using physiological and proteomic analyses.

168 of this model to experimental studies in the chemostat, using the model organisms Escherichia coli an

169 Using growth adaptation in a chemostat, we increased n-butanol tolerance to 15 g/L.

170 ng at different steady-state growth rates in chemostats were subjected to a short heat pulse.

171 Chemostats were used to adapt cells to different growth

172 Most of the chemostats where the fhuA polymorphism was lost also con

173 iption factors during steady-state growth in chemostats, which facilitated distinction of direct from

174 creased when T. paralvinallae was grown in a chemostat with 65 microM of added H2(aq) .

175 Using chemostat with cell retention (CCR) of Pseudomonas putid

176 rlo simulations of populations evolving in a chemostat with fixed washout rate and inflow resource de

177 ained in growing Escherichia coli 23716 in a chemostat with glucose as a limiting nutrient.

178 toxin/antitoxin hypothesis for bacteria in a chemostat with results incorporating the senescence hypo

179 pirical applications: (1) algal species in a chemostat with variable temperature, showing that the st

180 In equable environments (modeled here using chemostats with constant inputs of nutrients and sensiti

181 Cells were grown in chemostats with glucose, rate limiting or in excess, or

182 methanol or ethanol, during anoxic growth in chemostats with nitrate as the electron acceptor.

183 In variable environments (chemostats with seasonal inputs), bacteriophage with hig

184 hesis from nutrient signaling by growing, in chemostats, yeast auxotrophs for histidine, lysine, or u