ライフサイエンスコーパス: chromogenic substrate

1 he heightened affinity of MnO(2) NFs for the chromogenic substrate.

2 ,5'-tetramethylbenzidine (TMB) was used as a chromogenic substrate.

3 ld have to be deployed to build a fluoro- or chromogenic substrate.

4 y assaying their peroxidase activity using a chromogenic substrate.

5 with the pathogen of interest reacts with a chromogenic substrate.

6 onjugated antibody against digoxigenin and a chromogenic substrate.

7 competitive with respect to the tripeptidic chromogenic substrate.

8 amidolytic activities toward a FIXa-specific chromogenic substrate.

9 e on a monoclonal antibody and assaying with chromogenic substrate.

10 by commercially optimized assay coupled with chromogenic substrate.

11 thrombin activity that is measurable with a chromogenic substrate.

12 n 13 min using enzymatic amplification and a chromogenic substrate.

13 nd demonstrated acetylesterase activity with chromogenic substrates.

14 centration range compared with commonly used chromogenic substrates.

15 o five-fold when compared with commonly used chromogenic substrates.

16 t rely on the availability of radioactive or chromogenic substrates.

17 stantially lower levels of activity on other chromogenic substrates.

18 ability to cleave varied trypsin-susceptible chromogenic substrates.

19 Hydrolysis of the chromogenic substrate 4-methyl umbelliferyl-beta-galacto

20 lly been measured by staining cells with the chromogenic substrate 5-bromo-4-chloro-3-indolyl-beta-d-

21 blue-colony phenotype on plates containing a chromogenic substrate, 5-bromo-4-chloro-3-indolylphospha

22 oside phosphorylase coupling system with the chromogenic substrate 7-methylthioguanosine (MesG).

23 ive binding studies with a thrombin-specific chromogenic substrate, a fluorescein-labeled hirudin pep

24 ermediate, meizothrombin, that has increased chromogenic substrate activity but poor clotting activit

25 egraded approximately twice as fast than the chromogenic substrate, Ala-Pro-2naphthylamide, by dipept

26 larginine p-nitroanilide (Ac-Arg-pNA) as the chromogenic substrate all indicate a cooperative binding

27 The cleavage rates of both a small chromogenic substrate and the factor XIII activation pep

28 tivator was able to degrade both a synthetic chromogenic substrate and the natural substrate fibrinog

29 C may switch the enzyme specificity with the chromogenic substrates and inhibitors but not with the n

30 h a slow conformational change by tripeptide chromogenic substrates and its specific physiological su

31 or factor X, although the affinity for small chromogenic substrates and the efficiency of factor X sc

32 e EC activator did not hydrolyze a factor Xa chromogenic substrate, and recombinant tick anticoagulan

33 ed using a fluorescent thrombin inhibitor, a chromogenic substrate, and SDS-PAGE.

34 h overlapping specificities, enzyme-specific chromogenic substrates, and an antibody specific for act

35 Most chromogenic substrates are required in costly amounts, w

36 X was determined to be functional by using a chromogenic substrate assay after immunoprecipitation wi

37 The hPC was functional as determined by a chromogenic substrate assay after immunoprecipitation.

38 ine in factor VIII activity (measured with a chromogenic substrate assay) during weeks 49 through 52

39 Using chromogenic substrate assays, immunoblotting, and ELISA,

40 ion of time with radiolabeled proteins and a chromogenic substrate at resolutions of 2-8 s.

41 ortCorin exhibited catalytic activity toward chromogenic substrates but failed to cleave pro-ANP, ind

42 activation and the cleavage of a tripeptidic chromogenic substrate by factor XIa.

43 mpetitively inhibits the hydrolysis of small chromogenic substrates by factor Xa but binds in an orie

44 d that Na(+) stimulated the cleavage rate of chromogenic substrates by FXa or GDFXa approximately 8-2

45 nly a small effect on the hydrolysis of nine chromogenic substrates carrying substitutions at P1, P2,

46 found that all FIXa derivatives cleaved the chromogenic substrate CBS 31.39 with near normal catalyt

47 The fluorogenic substrates were compared to chromogenic substrates (CENTA, nitrocefin, and imipenem)

48 agar assay, zymography, and cleavage of the chromogenic substrate, Chromozym PL.

49 strated hyperbolic, mixed-type inhibition of chromogenic substrate cleavage by factor IXa (K(I) = 88

50 d complex increased 3-fold, and the level of chromogenic substrate cleavage by factor IXa increased m

51 by the factor IXa-phospholipid complex, and chromogenic substrate cleavage by factor IXa, only in th

52 DHG did not affect factor VIIIa half-life or chromogenic substrate cleavage by factor IXa-phospholipi

53 ith the active site, as it failed to inhibit chromogenic substrate cleavage by the factor IXa-PL comp

54 n or the ability of alpha-thrombin to cleave chromogenic substrates, clot fibrinogen, or block alpha-

55 d a variation of the two-stage assay using a chromogenic substrate (COAMATIC; 19% +/- 6.9% of FVIII W

56 Plasmin effectively cleaved a chromogenic substrate corresponding to this cleavage sit

57 higher in one-stage clot (OS) assays than in chromogenic-substrate (CS) assays, whereas recombinant F

58 Kinetic studies using the chromogenic substrate D-H-Pro-Phe-Arg-4-nitroanilide sho

59 Using chromogenic substrate, DrKIn-I dose-dependently inhibite

60 he mutant, instead, cleaved the APC-specific chromogenic substrates efficiently.

61 ody complexes on the blot are reacted with a chromogenic substrate (either 3.3'-diaminobenzidine [DAB

62 REG shows promise as a chromogenic substrate for E. coli detection and should b

63 A new chromogenic substrate for laccases and peroxidases, 1,8-

64 hylbenzothiazoline-6-sulfonate), is a common chromogenic substrate for peroxidase enzymes, which are

65 We propose a real-time assay based on a chromogenic substrate for phosphatase activity (pNPPase

66 p-nitroanilide), which can also be used as a chromogenic substrate for plasmin.

67 Here we have identified a new and effective chromogenic substrate for Tdp2 and developed a homogeneo

68 lactopyranosides, were found to be effective chromogenic substrates for an endo-alpha-N-acetyl-D-gala

69 ative micromethod that uses conventional and chromogenic substrates for the identification of medical

70 tivity as much as 70-fold in kcat/K(m) for a chromogenic substrate gamma-Glu-Ala-Gly-pNA.

71 comparison, thrombin-catalyzed hydrolysis of chromogenic substrates gives greater KSIEs and more comp

72 ously by continuous measurement of parabolic chromogenic substrate hydrolysis and fluorescence-based

73 These mutations only had modest effects on chromogenic substrate hydrolysis and the kinetics of fac

74 Chromogenic substrate hydrolysis by the mutant proteases

75 courses of Pg activation by SK monitored by chromogenic substrate hydrolysis had initial rates (v(1)

76 s curves of Pg activation by SK monitored by chromogenic substrate hydrolysis were parabolic, with in

77 y different mechanisms of Na+ enhancement of chromogenic substrate hydrolysis.

78 AP sites is estimated by HRP activity toward chromogenic substrate in an ELISA assay.

79 roups were also distinguishable by using the chromogenic substrates in Dade MicroScan test panels.

80 e, was assayed by the digestion of synthetic chromogenic substrates in protein-matched aliquots of ce

81 Three discontinuous chromogenic substrate initial rate assays were developed

82 ibitor of gamma-thrombin hydrolysis of small chromogenic substrate (K(i) = 0.694 +/- 0.063 nM).

83 several polyanions on the hydrolysis of the chromogenic substrate L-pyroglutamyl-L-prolyl-L-arginyl-

84 rostin, a serine protease, which cleaves the chromogenic substrate (N-benzoyl-L-Ile-L-Glu-L-Gly-L-Arg

85 Enzymes and a chromogenic substrate needed for colorimetric detection

86 producing inactive OXA-24 as assayed by the chromogenic substrate nitrocefin.

87 emperatures from 0 to 25 degrees C using the chromogenic substrate o-nitrophenyl-beta-galactoside.

88 s and products directly and does not require chromogenic substrates or lengthy chromatography, was su

89 tracts of these cells readily hydrolyzed the chromogenic substrate p-nitrophenyl-alpha-d-glucopyranos

90 enzyme activity was measured using ABTS as a chromogenic substrate (pH 6.0).

91 effects of the cofactor on the hydrolysis of chromogenic substrates probing the interior of the catal

92 ontrary to results in amidolytic assays with chromogenic substrates, prothrombinase is resistant to i

93 Herein, we propose an alternative chromogenic substrate, resorufin beta-d-glucuronide (REG

94 l 3 enzymes have similar activity toward the chromogenic substrate S-2238, that meizothrombin and mei

95 to the active protease plasmin, cleaved the chromogenic substrate S-2251 and the natural substrate f

96 n bound to the borrelial protein cleaved the chromogenic substrate S-2251 upon conversion by urokinas

97 Activation was monitored using a chromogenic substrate (S-2238) to detect both II(a) and

98 in a plasma clotting assay, to hydrolyze the chromogenic substrate S2366, and to undergo inhibition b

99 Kinetic analysis of these variants using chromogenic substrates showed differences in substrate s

100 with respect to their ability to cleave the chromogenic substrate Spectrozyme PCa, react with protei

101 at the A and B toxins of C. difficile cleave chromogenic substrates that have stereochemical characte

102 in; it used an enzyme-labeled antibody and a chromogenic substrate to provide an amplified visible si

103 Hydrolytic activity of the chromogenic substrate tosyl-glycyl-prolyl-argly-4-nitroa

104 cus faecium (mauve colonies) on the basis of chromogenic substrate use.

105 rhK4 cleaved the chromogenic substrates Val-Leu-Arg-pNA (S-2266), Pro-Phe

106 By utilizing indolepropionyl-CoA as a chromogenic substrate, we could measure the enzyme activ

107 lycosylated pseudoform for several synthetic chromogenic substrates were considerably less (33%-50%)

108 The cleavage of a chromogenic substrate with a single Arg residue at P1 wa

109 he activation was monitored by cleavage of a chromogenic substrate with newly formed plasmin, the rea

110 The chimeric mutant cleaved a FIXa-specific chromogenic substrate with normal catalytic efficiency,

111 er venom at similar rate, but it cleaved the chromogenic substrates with a Gly at the P2 positions po

112 T99Y mutant, similar to FXa, hydrolyzed the chromogenic substrates with a Gly at the P2 positions.

113 or assays, and activities against a range of chromogenic substrates with control plasma.

114 hundredth of the quantity needed for common chromogenic substrates, with an estimated bulk cost of <

115 s were seen in reactivity towards a range of chromogenic substrates, with substantial differences in

116 bitor 6 reduced V(MAX) of FXIa hydrolysis of chromogenic substrate without affecting the K(M), sugges

117 ning with a post-azo-coupling method using a chromogenic substrate (Z-arg-arg-MNA) or by a novel assa