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1 stage hyperinsulinemic euglycemic pancreatic clamp).
2 e and during the hyperinsulinemic-euglycemic clamp.
3 termined using a hyperinsulinemic-euglycemic clamp.
4 onditions with a hyperinsulinemic euglycemic clamp.
5 G-PET/MRI during hyperinsulinemic-euglycemic clamp.
6 ation in human atrial myocytes using dynamic clamp.
7 th through the CMG helicase-Pol epsilon-PCNA clamp.
8 at KGlu effects are not specific to the beta-clamp.
9 uctance via dual whole-cell/perforated patch clamp.
10 diomyocytes under field stimulation or patch clamp.
11 primed DNA, an incoming ddTTP, and the PCNA clamp.
12 WPB exocytosis, indicating that it acts as a clamp.
13 is expected to induce opening of the sliding clamp.
14 s as measured by hyperinsulinemic euglycemic clamps.
15 on the oligomerization properties of sliding clamps.
16 d functional T-type VGCC currents when patch clamped.
17 of ischemic conditioning before and/or after clamping.
18 ally been studied in low throughput by patch clamping.
19 se tolerance test and two-step hyperglycemic clamp (100 and 300 mg/dL) followed by intravenous argini
20 obese groups, received a euglycemic insulin-clamp (40 mU/m(2) . min) and an oral glucose tolerance t
21 vesicle, Synaptotagmin-1 oligomers bind and clamp a limited number of 'central' SNARE complexes via
24 s of diverse ABCG2 ligands using the pai-pai clamp along with structural studies created a pharmacoph
29 ptical glutamate sensors combined with patch-clamp and 3D molecular localization reveal that LTP indu
30 t clear.METHODSA hyperinsulinemic-euglycemic clamp and a 3-hour oral glucose tolerance test were perf
32 uring the first postnatal weeks, using patch-clamp and field recordings in mouse brain slices (C57Bl/
34 r antigen (PCNA), a homotrimeric DNA sliding clamp and polymerase processivity factor, is loaded onto
37 ctures reveal that TFEalpha bridges the RNAP clamp and stalk domains to open the DNA binding cleft.
39 embled excitable membranes using the dynamic clamp and voltage-gated potassium ionic channels (Kv1.3)
40 urrents were measured using whole-cell patch clamping and a fast perfusion system to assess fast (<2
43 safety of combined lithotripsy of mechanical clamping and electrohydraulics in fragmentation and remo
44 rs of insulin resistance in vivo (euglycemic clamps and HOMA of insulin resistance), and the presence
45 imultaneous membrane-current (ruptured patch-clamp) and [Ca(2+)](i)-recordings in atrial cardiomyocyt
46 nses in models of both warm ischemia (kidney clamping) and prolonged cold ischemia (syngeneic renal t
47 to prevent un-initiated fusion of vesicles (clamping) and to trigger fusion following Ca(2+)-influx.
48 ical, electrophysiological (whole-cell patch clamp), and behavioral approaches were used to examine t
49 r, L-type Ca(2+) current by whole-cell patch-clamp, and cardiomyocyte shortening and Ca(2+) transient
50 vity from a hyperinsulinemic-euglycemic (EU) clamp, and glucose counterregulatory responses from a su
52 yzed by a 2-step hyperinsulinemic euglycemic clamp, and postprandial interorgan crosstalk of lipid an
56 tive Syt1 oligomers, acting as an exocytosis clamp, are critical for maintaining the balance among th
58 rement with the plasma glucose concentration clamped at the fasting level; and 3) repeat EGP measurem
60 mice undergoing euglycemic-hyperinsulinemic clamps, being highly up-regulated in liver and down-regu
61 res linking allosterically the ATP, DNA, and clamp binding sites: residues distinctive of gamma and o
64 gands and antibodies for membrane proteins" (CLAMP), can yield ligands with high potency and specific
65 ore-operated Ca(2+) current I(CRAC) in patch-clamped cells, unlike Ins(1,4,5)P(3) consistent with res
66 nanopores works like a pincer to capture and clamp CO(2) and H(2) O precursors simultaneously, thus l
73 Insulin sensitivity during hyperglycemic clamp declined by ~30% and ~55% (both P < 0.05), respect
75 ing morphology was compared against a volume clamp device (CNSystems CNAP 500) (n = 3) resulting in s
76 iversal apparatus and methods with a special clamping device of non-thermal atmospheric plasma (NTAP)
77 iversal apparatus and methods with a special clamping device using gas mixtures are promising for imp
78 mM) in the pipet solution used during patch clamping did not recover NKA activity in the plumbagin o
79 logy including an extra "H" beta-strand and "clamping" disulfide, absent in known IgV-like structures
80 on trimer has three receptor-binding domains clamped down by a segment adjacent to the fusion peptide
83 age, Wistar rats underwent suprarenal aortic clamping either alone or in combination with short cycle
95 o transgenic reporter mice, whole-cell patch-clamp electrophysiology, and input-specific optogenetics
97 platform of physiological assays using patch-clamp electrophysiology, calcium imaging, and multielect
99 examine, using male C57BL6/J mice and patch-clamp electrophysiology, how chronic high-fat high-sugar
100 ng live-cell based techniques, such as patch-clamp electrophysiology, is laborious and time-consuming
109 Q1 antibodies and performed whole-cell patch clamp experiments as well as single-channel recordings o
117 combines mathematical modelling with dynamic clamp experiments, suggests the difference is due to the
118 ce energy transfer-based biosensors in patch clamp experiments, we discovered a robust voltage depend
123 d change/induced fit" mechanism in which the clamp first opens, followed by DNA binding, inducing ope
125 a combination of electrophysiology, voltage-clamp fluorometry, synthetic BigDyn analogs, and noncano
127 ion channel activity, recorded under voltage clamp, follows KfSLAC1 and KfALMT12 transcript abundance
129 trap MutS at the mismatch en route to mobile clamp formation and stop movement of the mobile clamp on
130 nking, we find that after the initial mobile clamp formation event, MutS undergoes frequent cycles of
134 eripheral (glucose uptake during the insulin clamp), hepatic (basal endogenous glucose production x f
136 monal conditions (studies using a pancreatic clamp), hyperglycemia suppressed EGP by ~50% in both hum
137 on rate/insulin (mg/kg/min) by hyperglycemic clamp in 50 adolescents with T1D (age 16.1 +/- 3.0 years
139 s transported into E-S/E-K compartments are 'clamped' in a sub-compartment created by Scc2's associat
140 olation of atrial myocytes, whole-cell patch clamping, in vitro tachypacing of atrial myocytes, lucig
142 e GIR required by ghrelin-KO mice during the clamps, increased plasma corticosterone and growth hormo
144 R ligands that mimic key native 'carboxylate clamp' interactions between Hsp90 and its TPR cochaperon
145 issociation equilibrium constant of the beta-clamp is of the order of 10 pM in buffers of moderate io
146 o 425 degrees C) and releasing the substrate clamping is shown to dramatically impact ferroelectric s
147 uppressed Ca(2+) oscillations, and the patch-clamped K(ATP) channel opened more frequently when gluco
149 a conformation before PCNA opening, with the clamp loader ATPase modules forming an overtwisted spira
153 rystal structure, suggesting that eukaryotic clamp loaders adopt a similar autoinhibited state early
155 yping, including hyperinsulinemic-euglycemic clamps, magnetic resonance spectroscopy, muscle biopsies
157 amplifiers, configurable into pseudocurrent-clamp mode (for concurrent current injection and voltage
158 and voltage recording) or into pseudovoltage-clamp mode (for concurrent voltage application and curre
162 ation and inactivation in intact loose-patch clamped murine skeletal muscle fibres subject to a doubl
165 eparations, site-directed mutagenesis, patch-clamp, nerve recordings and pharmacological inhibition t
167 ohistochemistry, Western blotting, and patch clamping of membrane potentials was performed to evaluat
169 Ca(2+) release sensor and as the inhibitor (clamp) of spontaneous and delayed asynchronous release.
170 currents were recorded in whole-cell voltage-clamped OHCs while electrically stimulating the MOC fibe
171 men underwent a euglycemic-hyperinsulinemic clamp on 2 separate days: one day with prior one-legged
176 ugh mutagenesis of the PCNA/Pol30 polymerase clamp or deleting specific error-prone polymerases aboli
177 als, membrane ion-currents (perforated patch-clamp), or simultaneous membrane-current (ruptured patch
180 Ubiquitylation of the eukaryotic sliding clamp, PCNA, activates a pathway of DNA damage bypass th
181 ucleic Acid (PNA) and Xenonucleic Acid (XNA) clamp PCR, enabling detection of low-frequency (<0.01%)
182 MCR(I) was calculated during the insulin-clamp performed with [3-(3)H]glucose and the OGTT and re
185 ned the effect of dapagliflozin on EGP while clamping plasma glucose, insulin, and glucagon concentra
186 stage hyperinsulinemic-euglycemic pancreatic clamp procedure in conjunction with glucose and palmitat
187 sessed using the hyperinsulinemic-euglycemic clamp procedure in conjunction with glucose tracer infus
189 oader replication factor C (RFC) and sliding clamp proliferating cell nuclear antigen (PCNA) are both
190 PVH neuron responsiveness, achieved through clamping PVH neuron activity at high or low levels, both
191 Using a combination of experimental (patch-clamp recording and Ca(2+) imaging at CA3-CA1 synapses)
193 Here we used Patch-seq(8) to combine patch-clamp recording, biocytin staining, and single-cell RNA
196 atory neurons, we performed multi-cell patch clamp recordings and Patch-seq on neurons derived from N
207 male and female ChAT-EGFP mice, and current-clamp recordings obtained from BFCNs chronically exposed
208 tween rod flash responses and recent voltage-clamp recordings of cone flash responses, using a model
209 test these possibilities, we combined patch-clamp recordings of ependymal cells with immunohistochem
213 behavioural hyperalgesia phenotype, voltage-clamp recordings of small and medium dorsal root ganglio
215 Additionally, we describe the first patch clamp recordings of VENs from neurosurgically-resected t
229 g rabies tracing, calcium imaging, and patch-clamp recordings, we show that corticofugal neurons proj
234 ponses from a subsequent hypoglycemic (HYPO) clamp.RESULTSLow and intermediate MMTT C-peptide groups
237 economically configuring a traditional patch-clamp rig for performing high signal-to-noise ex vivo ER
241 es key features of I(SK) recording during AP clamp showing that intrinsic rectification limits I(SK)
244 or several practices, including delayed cord clamping, stabilisation with intubation, early enteral f
245 , 3.21 to 10.84) in the surgery group during clamp stage 1, and by 5.39 (95% CI, 2.44 to 8.34) and 5.
246 nute in the two groups, respectively, during clamp stage 2; there were no significant differences bet
247 (disposition) index declined by 60% (second clamp step) and by 62% following arginine (both P < 0.00
248 0 min) and second (90-160 min) hyperglycemic clamp steps increased by 3.8-fold and 1.9-fold, respecti
250 Optogenetic studies in vivo and dynamic clamp studies in slice indicated that such brief PC supp
252 th the Saccharomyces cerevisiae PCNA sliding clamp, suggesting that KGlu effects are not specific to
253 male rats, we employed the action potential-clamp technique to determine the underlying ionic mechan
256 were combined with action potential voltage clamp techniques in a physiologically relevant heart fai
258 ed DNA structures, and becomes a sliding DNA clamp that can coordinate the subsequent repair reaction
259 er, our work suggests that FANCD2-FANCI is a clamp that is locked onto DNA by ubiquitin, with distinc
265 ted clamp and DNA interface enables the PCNA clamp to "waterskate" along the duplex with minimum drag
266 e-directed mutagenesis and single-cell patch-clamp to analyze the functional effect of the four still
267 We made use of a DNA origami-based force clamp to follow the assembly of human initiation complex
269 sing untagged NBCe1-A, injected with bCA and clamped to -40 mV, CO(2) / HCO3- exposures markedly decr
271 dividual frog hair bundles with displacement clamping to control hair bundle motion and measure force
272 of protein biochemistry and whole-cell patch clamping to determine effects of diet manipulation on gl
278 lative diffusion coefficients of two sliding clamps using fluorescence correlation spectroscopy.
282 oot ganglion (DRG), and the whole cell patch clamp was used to examine the amplitude of P2X currents
287 id's effect on I (Ks) Using whole-cell patch clamp, we show that mefenamic acid enhances I (Ks) activ
288 oral glucose tolerance tests and euglycemic clamps were performed, and histochemical analysis and ge
289 ctors present in the wild-type HLB including CLAMP, which normally binds to GAGA repeats in the H3-H4
290 o a deep amphipathic cleft, called the alpha clamp, which resides at the interface of two PA monomers
292 steatotic male participants to a pancreatic clamp with somatostatin and evaluated hepatic glucose an
293 20 mU/m(2)/min) hyperinsulinemic-euglycemic clamp with stable isotopes, in 6-week postpartum women w
294 r injection, the hepatoduodenal ligament was clamped with a vessel clip, and released 5 min later.
295 ced Ca(2+) entry when membrane potential was clamped with depolarization, the effect of TSPAN-7 on Ca
296 besity underwent hyperinsulinemic-euglycemic clamping with concomitant adipose tissue (AT) microdialy
297 ion on sensory hair cells, we combined patch clamping with fiber labeling in semi-intact cochlear pre
298 order involving 2-h euglycemic-hyperglycemic clamps with coadministration of: 1) SU (glimepiride 1 mg