ライフサイエンスコーパス: clasp

1 , and a neuromuscular abnormality (hind-limb clasping).

2 n and disengagement of the membrane-proximal clasp.

3 ctrostatic and hydrophobic interfaces in the clasp.

4 amino acids 550-554 acting as a hydrophobic clasp.

5 evolutionary conserved proteins, KATANIN and CLASP.

6 e-proximal methionine into a gp41-tryptophan clasp.

7 ins significantly loosens the inner membrane clasp.

8 domain-containing proteins and MT stabilizer CLASPs.

9 exhibited reduced body weight and hind limb clasping.

10 ddition to the previously described hindlimb clasping.

11 apical meristem activity, as observed in the clasp-1 null mutant.

12 ely conserved microtubule-associated protein CLASP [3].

13 R1) associates with the TOGL1 domain of Stu1(CLASP), a conserved plus-end microtubule protein that is

14 ce show behavioral abnormalities including a clasping abnormality of their hind limbs and a habituati

15 These results indicate that C. elegans CLASPs act partially redundantly to regulate astral micr

16 on protein PHLDB2/LL5beta.(4)(,)(5) Cortical CLASPs also stabilize a subset of microtubules, which st

17 We believe that our orthogonal clasp analysis provides a building block for future mode

18 ns during axon guidance and antagonizes both CLASP and Abl activity.

19 we report that Msps is a strong modifier of CLASP and Abl in the retina.

20 omplementarity with a PARG-specific tyrosine clasp and arginine switch, supporting inhibitor specific

21 ments required for entry involve opening the clasp and expelling the termini.

22 the hPol kappa by interactions between the N-clasp and finger domains combined with stabilization of

23 Our data suggest a model in which CLASP and Msps converge in an antagonistic balance in th

24 e yield a refined model for the alphav beta3 clasp and provide plausible explanations for the effects

25 The clasp and second helix amino dipole recognize a central

26 constrained by the close apposition of the N-clasp and the fingers domain, and therefore can accommod

27 ctions biomechanically as an inter-molecular clasp and thereby facilitates supra-molecular assembly.

28 module and the Fc region facilitated antigen clasping and achieved both high specificity and high pot

29 inclusion size, and lowered the frequency of clasping and footslips on balance beam.

30 ncluding survival, body weight, tremor, limb clasping and open field activities.

31 XMAP215, CLASP, and Crescerin use arrayed tubulin-binding tumor o

32 tubule-associated genes, such as MAP70-5 and CLASP, and receptor genes, such as HERK1 and WAK1, were

33 subunits that is known as the outer membrane clasp, and the key interaction group of the betaA domain

34 m that sustains meristem homeostasis through CLASP, and they advance our understanding of how roots m

35 nomatous polyposis coli, cytoplasmic dynein, CLASPs, and LIS-1, has been shown recently to target to

36 our platform to generating high-performance clasping antibodies to diverse PTMs.

37 We further generated clasping antibodies to phosphotyrosine antigens by using

38 We designed the platform that generates clasping antibodies with two distinct binding units, res

39 application in epigenomics, revealed that a clasping antibody to trimethylated histone H3 at lysine

40 domain array-containing proteins ch-TOG and CLASP are key regulators of cytoplasmic MTs.

41 CLASPs are also recruited to the trans-Golgi network (TG

42 CLASPs are conserved MT-binding proteins implicated in t

43 reas the interaction sites with Clip-170 and CLASPs are dispensable.

44 rt, and cell motility.(7) In dividing cells, CLASPs are essential at kinetochores for efficient chrom

45 CLIP-associated proteins CLASPs are mammalian microtubule (MT) plus-end tracking

46 CLASPs are microtubule-associated proteins that have a c

47 We show that CLASPs are recruited to the trans-Golgi network (TGN) at

48 CLASPs are spatially regulated microtubule plus end trac

49 Furthermore, we show that Mad3(BUBR1)-Stu1(CLASP) are essential to rescue the segregation of mini-c

50 Cytoplasmic Linker Associated Proteins (CLASPs) are a conserved class of MT-associated proteins

51 Cytoplasmic linker-associated proteins (CLASPs) are MT-associated proteins thought to organize i

52 Cytoplasmic linker-associated proteins (CLASPs) are proposed to function in cell division based

53 ase 3beta (GSK3beta) directly phosphorylates CLASPs at multiple sites in the domain required for MT p

54 veals encirclement of the DNA by a unique "N-clasp" at the N terminus of Pol kappa, which augments th

55 al studies, KI mice did not display the foot-clasping behavior upon lifting by the tail and lacked an

56 vior and gait dynamics), corrects repetitive clasping behavior, as well as normalizes cued fear-condi

57 d motor deficits, as assessed by rotarod and clasping behavioural tests.

58 This signal, found in the CLASPs beta-arrestin and the autosomal recessive hyperch

59 rker of exposure of residues involved in the clasp between alpha(IIb) and beta(3) cytoplasmic tails,

60 Breakage of a C-terminal clasp between the alpha and beta subunits enhances Mn(2+

61 ) His(722), involved in the formation of the clasp between the tails are also required for skelemin b

62 The peptide is clasped between the N-terminal domain and the transmembr

63 SM proteins, shaped like clasps, bind to trans-SNARE complexes to direct their fu

64 Cell identifies the protein LL5beta as a key CLASP binding platform that mediates communication betwe

65 AP-2 beta2 subunit appendage is a privileged CLASP-binding surface that recognizes a cognate, short a

66 CLASP binds stably to the MT lattice, recruits tubulin,

67 cause they appear to mediate facilitation of clasping by AVT.

68 h, although Peg1 resembled higher eukaryotic CLASPs by physically associating with both Mal3 and Tip1

69 r how ARH can act as an endocytic adaptor or CLASP (clathrin-associated sorting protein) to couple LD

70 no longer localizes to chromosomes, whereas CLASP(CLS-2) depletion does not prevent HCP-1/2 targetin

71 1/2, two redundant CENP-F-like proteins, and CLASP(CLS-2) in Caenorhabditis elegans.

72 apart in the 1 cell embryo, indicating that CLASP(CLS-2) is required for biorientation when chromoso

73 HCP-1/2 and CLASP(CLS-2) localize transiently to mitotic C. elegans

74 In embryos depleted of HCP-1/2, CLASP(CLS-2) no longer localizes to chromosomes, whereas

75 hemical association, depletion of HCP-1/2 or CLASP(CLS-2) resulted in virtually identical defects in

76 sh that the key role of HCP-1/2 is to target CLASP(CLS-2) to kinetochores, and they support the recen

77 le antiparallel bundler PRC1/Ase1 to recruit CLASP/Cls1 to stabilize microtubules.

78 show here that the Schizosaccharomyces pombe CLASP, Cls1p, is a homodimer that binds an alphabeta-tub

79 , we show that the Schizosaccharomyces pombe CLASP, cls1p/peg1p, mediates the stabilization of overla

80 tely, we are interested in understanding how CLASP collaborates with functionally linked proteins to

81 l domain (CTD).(2)(,)(3) In migrating cells, CLASPs concentrate at the cortex near focal adhesions as

82 assessments, including analysis of gait and clasping, confirm the presence of a neurological defect.

83 The cofolded complexes have similar "zinc clasp" cores that are augmented by distinct structural e

84 provide a structural mechanism by which the CLASP CTD directs diverse sub-cellular localizations thr

85 and conserved arginine residue essential for CLASP CTD interaction with partner proteins.

86 CLASPs (cytoplasmic linker-associated proteins) are ubiq

87 ed of BUB-1(Bub1), HCP-1/2(CENP-F) and CLS-2(CLASP))-dependent pushing acts redundantly with Ndc80 co

88 growing microtubules reaching the cortex in CLASP depleted embryos, but the polymerization rate of a

89 Increased GTP-tubulin content within MTs in CLASP-depleted cells suggests that CLASPs facilitate GTP

90 In CLASP-depleted cells, EBs localized along the MT lattice

91 In vitro assays revealed that CLASP directly functions to remove EB from MTs.

92 IP-170, but in the leading edge of the cell, CLASPs display lattice-binding activity.

93 CLASP disruption also causes a marked detachment of MTs

94 SPs to kinetochores,(10)(,)(11) although the CLASP domain required for this interaction is not known.

95 of striatal pathology, begins with hind-limb clasping during tail suspension and tail stiffness durin

96 -arm grooming in which the grooming partners clasp each other's raised palms.

97 on a centrosome-to-ncMT transition requiring CLASP, EB1, and Patronin function.

98 mice developed forelimb stereotypy, hindlimb clasping, excessive grooming and hypo-activity prior to

99 in MTs in CLASP-depleted cells suggests that CLASPs facilitate GTP hydrolysis to reduce EB lattice bi

100 redundant CENP-F orthologs HCP-1/2, and the CLASP family member CLS-2 (hereafter termed the BHC modu

101 A CLASP family MT stabilizer and the depolymerizing kinesi

102 s the Saccharomyces cerevisiae member of the CLASP family of microtubule plus-end tracking proteins a

103 crotubule assembly requires targeting of the CLASP family protein CLS-2 to the kinetochores in metaph

104 The hormone is bound in a hand-clasp fashion to an elongated, curved receptor.

105 am, behavioral seizures, and marked hindlimb clasping; females displayed thigmotaxis in an open field

106 cter (LOS) with the anatomical gastric sling-clasp fibres at the oesophago-cardiac junction (OCJ).

107 inase 3beta likely regulates the affinity of CLASPs for microtubule lattices.

108 Cytoplasmic linker-associated proteins (CLASPs) form a conserved family of microtubule-associate

109 thened by cinching of a reciprocal "tyrosine clasp" formed between the N-terminal domain of TIMP-1 an

110 ing rescue while suppressing catastrophe.(1) CLASP function involves an ordered array of tumor overex

111 hey support the recently proposed model that CLASP functions to promote the polymerization of kinetoc

112 30, which remains anchored in the tryptophan clasp (gp41 W623, W628, W631) in the B41 Env prefusion s

113 ture of the Nse5/Nse6 core further reveals a clasped-hand topology and a dimeric interface important

114 Thus, the two TOGL domains of yeast CLASP have different activities and execute distinct mit

115 Structure predictions suggest that CLASPs have at least two TOGL domains.

116 f designed RNA origami 6-helix bundle with a clasp helix (6HBC).

117 ce motif, we define a novel structural Helix-Clasp-Helix (HCH) nucleotide binding motif and show ASCC

118 h nucleic acid (NA) and define a novel Helix-clasp-Helix-Strand-Loop (HcH-SL) NA recognition motif bi

119 ormational changes observed near the 'Ca(2+) clasp' hint at the mechanism of Ca(2+)-dependent gating.

120 ming in embryos depleted of either the orbit/CLASP homolog, CLS-2, or FZY-1.

121 Caenorhabditis elegans has three CLASP homologs in its genome.

122 nd beta-subunits known as the inner membrane clasp, hydrophobic packing of a few transmembrane residu

123 The data suggest that CAND1 clasps idling catalytic domains of an inactive SCF, roll

124 Valve RePair System Pivotal Clinical Trial [CLASP IID] NCT03706833).

125 Absence of the N-clasp in Dpo4 explains the error-prone processing of the

126 Here, we examine the dynamics of the +TIP CLASP in migrating PtK1 epithelial cells.

127 in, confirming the role of the extracellular clasp in restraining integrin activation.

128 esolution imaging of the Orbit/MAST ortholog CLASP in Xenopus growth cones suggests that this family

129 sembling a baseball catcher's mitt with heme clasped in the palm.

130 Notably, hind limb clasping in Abcd1-/y mice was corrected through transduc

131 e in rotarod performance, and alleviation of clasping in R6/2 mice, establishing a proof-of-principle

132 tubule-growth and nucleation agents, Ran and CLASP, in the establishment of the centrosome-independen

133 In vitro, CLASP increases MT rescue frequency, decreases MT catast

134 Together, these findings suggest that CLASPs influence the MT lattice itself to regulate EB an

135 llow us to precisely classify the roles that CLASP-interacting genes play in MT regulation.

136 g normal EB localization, whereas neither EB-CLASP interactions nor EB tail-binding proteins are invo

137 comprehensive survey of the proteins in the CLASP interactome as MT regulators is missing.

138 rallel genetic and proteome-wide screens for CLASP interactors in Drosophila melanogaster.

139 While the influence of some CLASP interactors on MT behavior is known, a comprehensi

140 viously described functions of several known CLASP interactors, its multiparametric resolution reveal

141 previously identified to be in vivo genetic CLASP interactors.

142 We show that while a point mutation in the clasp interface modestly activates alphaIIb beta3, addit

143 ance of chromosomes (SMC) family of ATPases, clasped into topologically closed rings by accessory sub

144 n of this mechanistically distinct subset of CLASPs into clathrin-coated buds.

145 Our results show that the pol kappa N-clasp is a key structural feature that accounts for the

146 Here, we investigated whether CLASP is involved in light-dependent root growth promoti

147 MT association of CLASPs is suggested to be regulated by multiple TOG (tum

148 with a membrane-proximal cytoplasmic domain clasp, is thought to maintain integrins in a low affinit

149 l microtubule stabilization sites containing CLASPs, KIF21A, LL5beta and liprins are recruited to foc

150 irectly to microtubules and are required for CLASP localization to kinetochores.

151 race of an ABO fucose residue by a disulfide-clasped loop, which is inactivated by reduction.

152 rticular, evidence suggests that the MT+TIP, CLASP, may play a pivotal role in the coordination of mi

153 ppears to compensate for release of SPs that clasp MCP capsomeres together.

154 er and as a dimer and further defines that a clasp mechanism may control lipid binding and, ultimatel

155 w functional residues that participate in a "clasp" mechanism to modulate apoA-IV lipid affinity.

156 This indicates that CLASP-mediated stabilization of peripheral MTs, which li

157 tubule (MT) plus-end tracking protein (+TIP) CLASP mediates dynamic cellular behaviors and interacts

158 mediated by the COOH-terminal region of the CLASP microtubule-binding domain and is regulated downst

159 mation of microtubules at the Golgi requires CLASPs, microtubule-binding proteins that selectively co

160 Our work suggests that the clasp might be involved in enzyme kinetics, with the N-t

161 microtubule polymerization (EB1, Mast/Orbit [CLASP], Minispindles [Dis1/XMAP215/TOG]) or depolymeriza

162 that cytoplasmic linker-associated proteins (CLASPs) modulate EB localization at MTs.

163 Our data identify the clasp motif as a fundamental contributor to CA-CTD inter

164 This arginine clasp motif can robustly mediate cohesive interactions b

165 nesis, but the specific requirements for the clasp motif in several steps of M-PMV particle assembly

166 we report an examination of the role of the clasp motif in the M-PMV life cycle.

167 stretch of 5 amino acid residues termed the "clasp motif," important for the organization of the hexa

168 We show that a short "clasp" motif in the capsid domain of the M-PMV Gag prote

169 Beside the well known lock-and-key and clasp motifs, other alternative structural interactions

170 LOS' that likely reflects the gastric sling/clasp muscle fibres at the OCJ.

171 it(+) cells (ICC-IM) in mouse and monkey LES clasp muscles.

172 The same is true in orbit/clasp mutants, indicating a pivotal role for this microt

173 reproductive behaviors, including courtship clasping of females.

174 tary movements with dystonic-appearing, self-clasping of limbs, as early as 3 weeks after birth.

175 aning are healthy, but they show an abnormal clasping of the hindfeet when suspended by the tail.

176 Thus, the impact of S. pombe CLASP on interphase microtubule behavior is more closely

177 Here, we investigate the effects of CLASPs on the pre-catastrophe intermediate state of micr

178 perturbs the electrostatic interface in the clasp only partially activates alpha(IIb)beta(3) and tha

179 With VIR-CLASP, only the incoming virion RNAs are labeled with 4S

180 rogen-bonded carboxyl-carboxylate clamp that clasps onto a charged Lys side chain.

181 eins that locally alter MT dynamics, such as CLASP or kinesin-4.(7)(,)(13)(,)(14)(,)(15)(,)(16) Howev

182 Disrupting ncMT function by compromising CLASP or Patronin function leads to failures in nuclear

183 oplasmic linker protein)-associated protein (CLASP), originally identified as a MT plus end-binding p

184 s on a region of the protein that produces a clasp over the active site.

185 behavioral abnormalities including hind limb clasping, overt seizures, motor impairment and context-

186 charomyces pombe CLIP170-associated protein (CLASP) Peg1 was identified in a screen for mutants with

187 g the Dlx5/6-Cre transgene, led to a hindpaw-clasping phenotype and a 50% loss of MSNs without affect

188 Adult Emx-BDNF(KO) mice display a hindlimb clasping phenotype similar to that observed in mouse mod

189 improved the locomotor deficits and hindlimb clasping phenotype, both in male and female mice, and fu

190 omerulus size, and a characteristic hindlimb-clasping phenotype.

191 ddition, In-M-cko demonstrated a severe limb-clasping phenotype.

192 e tremor, and most animals showed a hindlimb clasping phenotype.

193 test, and increased spasticity as shown by a clasping phenotype.

194 However, both lines developed spasticity (a "clasping" phenotype) at a median age of 21 wk and 29 wk,

195 re more active and developed severe hindlimb clasping phenotypes.

196 Palm-to-palm clasping (PPC) is a distinct style of high-arm grooming

197 mice and male Japanese quail), reproductive clasping (pre-copulatory mounting in newts), and paced m

198 This "antigen clasping" produced an expansive interface where trimethy

199 We showed that CLASP protein levels were greatly reduced in the root ti

200 ntrinsically disordered region of vertebrate CLASP proteins contains two SXIP EB1 binding motifs that

201 for microtubule nucleation at the Golgi via CLASP proteins.

202 gic phenotype including hunchback, hind limb clasp, reduced survival and brain and cortical neuron en

203 otypes including ataxia, front and hind limb clasping, reduced brain size, and smaller neurons.

204 in growth rate and exhibited an altered leg clasp reflex, an altered gait, and defective nursing beh

205 gat3(Delta) mutations exhibited a marked leg clasp reflex, indicating that in the absence of wild-typ

206 Mutation of the clasp region of alphav or beta3 results in a constitutiv

207 arge domain relative to the small domain and clasp region within each subunit of the dimeric enzyme.

208 he large domain relative to the small domain/clasp region, reminiscent of what has been observed in t

209 peptides representing the alphaIIb and beta3 clasp regions promote integrin activation as judged by c

210 Cytoplasmic linker-associated proteins (CLASPs) regulate microtubules in fundamental cellular pr

211 l. show that the microtubule binding protein CLASP regulates PIN2 auxin transporter trafficking and s

212 inds that the microtubule-associated protein CLASP repairs lattice damage by regulating GTP-tubulin i

213 sed MT regulatory paradigm beyond ch-TOG and CLASP, representing a distinct regulator of cilia struct

214 While both gamma-TuNA inhibition and lack of CLASPs resulted in drastically decreased GDMT nucleation

215 henotype of incoordination, involuntary limb clasping, seizures, and premature death.

216 eta, cytoplasmic linker-associated proteins (CLASPs), specialized host +TIPs that control MT formatio

217 How CLASPs specifically modulate microtubule transitions is

218 CLASPs stabilize dynamic microtubules by suppressing mic

219 Decortication ameliorated hindlimb clasping, striatal neuron atrophy, and huntingtin-positi

220 RRM1, influenced by a newly identified 'beta-clasp' structure.

221 that only TOGL2 of Saccharomyces cerevisiae CLASP Stu1 binds to tubulin and is required for polymeri

222 Antigen clasping substantially expands the paradigm of antibody-

223 Therefore, we conclude that CLASPs suppress microtubule catastrophe by stabilizing t

224 owed that the microtubule-associated protein CLASP sustains root apical meristem size by influencing

225 n of 4SU-labeled virus takes ~7 days and VIR-CLASP takes 1 day.

226 ts on the accelerating rotorod and hind limb clasping tests in transgenic HD mice.

227 helices extend into the cytoplasm and form a clasp that differs significantly from a recently publish

228 ing of the alpha and beta TM domains forms a clasp that regulates integrin activation.

229 s the Gly-X-X-Gly (GXXG) signature sequence "clasp" that brings together two helices as an ~90 degree

230 le the assembly of outer and inner membrane 'clasps' that hold the alphabeta TMD together to limit tr

231 2) is a clathrin-associated sorting protein (CLASP) that contributes to clathrin recruitment, vesicle

232 ent of clathrin-associated sorting proteins (CLASPs) that independently select different integral mem

233 ical domain and terminal, extended arms that clasp the compact ASCC2 unit.

234 at terminates in two beta-sandwiches tightly clasping the glucosyltransferase domain.

235 s of GK undergo the same closure motion that clasps the transition state analogue, in contrast to the

236 Previous studies found that "clasping" the alphaIIb head domain to the beta3 tail dec

237 Cell, Efimov and colleagues report a role of CLASPs, the MT plus end-binding proteins, in MT formatio

238 t the border of the outer and inner membrane clasps, thereby decoupling the tilt between these segmen

239 stimuli depends on the ability of Galpha to clasp tightly the GTP molecule that enters the binding s

240 three specificity-conferring NetrinG loops, clasped tightly by matching NGL surfaces.

241 A clasp to mimic juxtamembrane association between the int

242 and beta3 function as a novel extracellular clasp to restrain activation.

243 ,)(9) Both CENP-E and ASTRIN bind and target CLASPs to kinetochores,(10)(,)(11) although the CLASP do

244 ss-linking and solid-phase purification (VIR-CLASP) to characterize the earliest interactions between

245 ut fail to biorient rely on Mad3(BUBR1)-Stu1(CLASP) to ensure their efficient attachment to microtubu

246 Mutations in the CLASP TOG domains demonstrate that tubulin binding is cr

247 The single-arm, multicenter, prospective CLASP TR (Edwards PASCAL TrAnScatheter Valve RePair Syst

248 he U.S. single-arm, multicenter, prospective CLASP TR early feasibility study of the PASCAL transcath

249 (Edwards CLASP TR EFS [CLASP TR EFS]; NCT03745313).

250 (Edwards CLASP TR EFS [CLASP TR EFS]; NCT03745313).

251 gitation [CLASP TR] Early Feasibility Study [CLASP TR EFS]; NCT03745313).

252 ve RePair System in Tricuspid Regurgitation [CLASP TR] Early Feasibility Study [CLASP TR EFS]; NCT037

253 ve RePair System in Tricuspid Regurgitation [CLASP TR] Early Feasibility Study) evaluated 1-year outc

254 We find that, although CLASPs track microtubule plus ends in the cell body, the

255 In migrating epithelial cells, CLASPs track MT plus ends in the cell body but bind alon

256 dentified GSK3beta motifs determines whether CLASPs track plus ends or associate along MTs.

257 , we found that despite the lack of protein, CLASP transcript levels were higher in dark-grown root t

258 We propose a model in which CLASP transduces GSK3 activity levels to differentially

259 We propose a mechanism for rescue in which CLASP-tubulin dimer complexes bind along the MT lattice

260 In the orthogonal clasp, two filaments are brought into contact, with each

261 ctivation of integrin adhesion receptors via clasping/unclasping of their membrane-proximal helices.

262 nal helix may regulate the equilibrium-based clasping/unclasping process.

263 Kcne3(-/-) mice exhibited abnormal hind-limb clasping upon tail suspension (63% of Kcne3(-/-) mice >/

264 (2D2(+) mouse) that presents with hind-limb clasping upon tail suspension and is associated with T c

265 hain of this asparagine forms an active site clasp via two H-bonds with the residue (Ser85) adjacent

266 We established VIR-CLASP (VIRal Cross-Linking And Solid-phase Purification)

267 The MT-binding region of CLASP was sufficient for restoring normal EB localizatio

268 of high-arm grooming featuring palm-to-palm clasping - we found that matrilineal relationships expla

269 eper insight into the functional partners of CLASP, we conducted parallel genetic and proteome-wide s

270 transporters, both of which are sustained by CLASP, were largely degraded in the dark.

271 nal neurons, which play an important role in clasping, were identified by retrograde labeling with te

272 binding mode of anti-PTM antibodies, antigen clasping, where two antigen binding sites cooperatively

273 ed a relapsing-remitting course of hind-limb clasping with the development of progressive motor defic

274 A "Ca(2+) clasp" within the channel's intracellular region acts as