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1 uding those that regulate disassembly of the clathrin coat.
2 o the correct spatiotemporal assembly of the clathrin coat.
3 the cell surface and link them to the PM and clathrin coat.
4 of clathrin-binding adaptor proteins and the clathrin coat.
5 ished for clathrin-AP-1/AP-2 coats, to a non-clathrin coat.
6 organization of the inner AP-1 layer of the clathrin coat.
7 regulation to ensure the correct assembly of clathrin coats.
8 on structural elements with COPI, COPII, and clathrin coats.
9 play central roles orchestrating assembly of clathrin coats.
12 h a perturbation of the coupling between the clathrin coat and the actin cytoskeleton, which we confi
14 oated vesicle, or with an in vitro assembled clathrin coat, and recruits Hsc70 to its specific heavy-
15 yotes, and many of the proteins required for clathrin coat assembly and disassembly have orthologs in
17 functions in clathrin-mediated endocytosis, clathrin coat assembly protein AP180, was quantified as
18 ribosylation factor 1 (ARF1), essential for clathrin coat assembly, Golgi architecture, and vesicula
20 s was not accompanied by the accumulation of clathrin coated buds on their surface and this process p
21 ch in metazoans links endocytic cargo to the clathrin coat, but had no assigned function in yeast, wa
22 tein 1 (ACAP1) functions as an adaptor for a clathrin coat complex that has a function in endocytic r
25 d the genes encoding a set of early arriving clathrin-coat constituents, FCHO1 and FCHO2, in HeLa cel
26 Inducible overexpression of the Arabidopsis clathrin coat disassembly factor, Auxilin2, which inhibi
32 e fission, which resulted in accumulation of clathrin-coated endocytic intermediates on the plasma me
33 h Vps class C/HOPS subunits incorporate into clathrin-coated endosomal domains and carriers in mammal
35 l-free reconstitution system, we report that clathrin coat formation and cargo sorting can be uncoupl
37 -active processes-vacuolar acidification and clathrin-coat formation--as modulators of sertraline's a
38 nistic models for disassembly of nonneuronal clathrin coats has been limited by the absence of a func
39 of the heat shock response or disassembly of clathrin coats, however, where binding of a short hydrop
40 at about 11 A resolution, the structure of a clathrin coat (in the D6-barrel form) with specifically
43 cytic defects and a striking accumulation of clathrin-coated intermediates, strongly implicating Sac
44 ecreased dynamin recruitment to the necks of clathrin-coated invaginations resulting in impaired vesi
45 tor protein 4 (AP-4) is a component of a non-clathrin coat involved in protein sorting at the trans-G
49 or protein 2 (AP2) complexes, which initiate clathrin-coated pit (CCP) assembly, are activated by con
52 ighly sensitive and quantitative analysis of clathrin-coated pit (CCP) dynamics, we have evaluated th
53 This corresponded to a decreased rate of clathrin-coated pit (CCP) initiation and increased lifet
54 hat this phosphorylation event starts during clathrin-coated pit (CCP) initiation and increases throu
55 DEO ABSTRACT: Some endocytic cargoes control clathrin-coated pit (CCP) maturation, but it is not know
59 synthesis and its metabolic processing, the clathrin-coated pit endocytosis pathway, and the ubiquit
65 r size increases beyond the size of a single clathrin-coated pit, B cells retrieve receptor clusters
67 after a 4 h chase and became undetectable if clathrin-coated pit-mediated trafficking was blocked wit
68 Classical CME proceeds via the formation of clathrin-coated pits (CCPs) at the plasma membrane, whic
69 ations affect the morphology and kinetics of clathrin-coated pits (CCPs) by directly following their
71 ns (EAPs) mediate assembly and maturation of clathrin-coated pits (CCPs) into cargo-containing vesicl
72 guishing abortive coats (ACs) from bona fide clathrin-coated pits (CCPs) is required but unaccomplish
74 es of the observed heterogeneous dynamics of clathrin-coated pits (CCPs) might be the different cargo
75 ed by stabilization and growth/maturation of clathrin-coated pits (CCPs) that eventually pinch off an
76 iated endocytosis occurs via the assembly of clathrin-coated pits (CCPs) that invaginate and pinch of
77 ntrol its fate by regulating the dynamics of clathrin-coated pits (CCPs) that mediate their internali
78 ing structures at the plasma membrane termed clathrin-coated pits (CCPs) that mediate vesicle formati
79 substantial increase in the ratio of "open" clathrin-coated pits (CCPs) to "necked"/"closed" CCVs an
80 composition of the lifetime distributions of clathrin-coated pits (CCPs) to measure independent aspec
81 P(2) levels and is concentrated at endocytic clathrin-coated pits (CCPs) via interactions with the sc
83 cargos are known to accumulate into maturing clathrin-coated pits (CCPs), whether and how cargo recru
87 that results in an accumulation of arrested clathrin-coated pits and a greatly reduced synaptic vesi
88 e clathrin-mediated endocytosis with shallow clathrin-coated pits and a strong reduction in the inter
91 tic adaptor, which is highly concentrated at clathrin-coated pits and coordinates acquisition of bila
93 hat ADAM17 is constitutively internalised by clathrin-coated pits and that physiological stimulators
94 per-resolution images of living cells, using clathrin-coated pits and the transferrin cargo as model
96 ns unclear whether the cargos in the growing clathrin-coated pits are actively monitored by the coat
97 eptor signaling, in cells in which endocytic clathrin-coated pits are frozen at a deeply invaginated
99 ation of EETI-II-positive macropinosomes and clathrin-coated pits at early time points after treatmen
101 catalyzes the scission of deeply invaginated clathrin-coated pits at the plasma membrane, but the mec
104 ns can be marked as cargo for inclusion into clathrin-coated pits by common internalization signals (
108 is generally believed that the formation of clathrin-coated pits in epithelial cells occurs randomly
109 y ligands, MORs are rapidly internalized via clathrin-coated pits in heterologous cells and dissociat
113 ocalization of the SNX9.dynamin-2 complex to clathrin-coated pits is blocked by interactions with the
114 inositol 3-kinase C2alpha at plasma membrane clathrin-coated pits is spatially segregated from its hy
115 d capsids laterally diffused into assembling clathrin-coated pits less than 30 s after attachment.
117 rin receptors are delivered selectively from clathrin-coated pits on the plasma membrane into a speci
118 e ESCRT-0 complex accumulates at a subset of clathrin-coated pits on the surface of human cells.
119 ocal activity, and axonal boutons containing clathrin-coated pits showed a more pronounced decrease i
120 erized, and it remains controversial whether clathrin-coated pits specialize to internalize particula
121 tosis depends on the formation of functional clathrin-coated pits that recruit cargos and mediate the
122 indings reveal a link between progression of clathrin-coated pits to endocytic vesicles and an activa
123 , these findings indicate that BRAG2 acts at clathrin-coated pits to promote integrin internalization
124 Superresolution imaging of microtubules and clathrin-coated pits was demonstrated, under both modes.
125 D1A, which encodes a protein associated with clathrin-coated pits where cell-surface receptors reside
127 PS15, a protein required for the assembly of clathrin-coated pits, and DN PAK-1, an obligate mediator
128 Triad3A associates with Arc, localizes to clathrin-coated pits, and is associated with endocytic s
129 oenvironments, three-dimensional tracking of clathrin-coated pits, and long-term imaging spanning >10
130 Lp(a) internalization was also dependent on clathrin-coated pits, and Lp(a) was targeted for lysosom
131 s, focal adhesions, primary cilia, caveolae, clathrin-coated pits, and plaques play additional key ro
132 1 and inducing GLUT1 internalization through clathrin-coated pits, as well as indirectly, by reducing
134 onstruct of EPS15, an essential component of clathrin-coated pits, blocked the entry of RRV into RFs.
135 sion by forming a collar around the necks of clathrin-coated pits, but the specific structural intera
136 thrin interactor, is recruited to late-stage clathrin-coated pits, clinical manifestations have been
137 st partially defined by the cytoskeleton and clathrin-coated pits, in which receptors and G proteins
138 )P(3) biosensors, disappearance of endocytic clathrin-coated pits, nearly complete inhibition of KCNQ
140 n thought to coordinate cargo selection into clathrin-coated pits, results in a significant impairmen
142 endocytosis of ligand-receptor complexes via clathrin-coated pits, trafficking of the internalized li
143 molecules via endocytic components, such as clathrin-coated pits, vacuoles, and micropinocytic vesic
144 ntrast to INPP5B visits late stage endocytic clathrin-coated pits, was earlier shown to contain anoth
145 ns in a complex with the GTPase dynamin-2 at clathrin-coated pits, where it provokes fission of vesic
146 accumulate at the base of arrested endocytic clathrin-coated pits, where they support the growth of d
147 -coated vesicles and an increase in U-shaped clathrin-coated pits, which may result from sequestratio
148 ntly, ligand-bound EGFR is incorporated into clathrin-coated pits--membrane structures containing cla
164 rin-mediated endocytosis and associates with clathrin-coated pits/vesicles at the plasma membrane.
165 as key membrane determinants for assembly of clathrin coat proteins that drive formation of clathrin-
166 f endocytosis following MC4R localization to clathrin-coated sites and exclusion of the receptor from
168 This combined approach has strong effects on clathrin coat structure and function by dictating the st
170 ar organisms on formation and dissolution of clathrin-coated structures (CCSs) have not been directly
171 we found that actin patches associated with clathrin-coated structures (CCSs) in cultured mouse cell
173 oth the large insert isoform of myosin VI on clathrin-coated structures and the no-insert isoform on
174 Further, the steady-state morphology of clathrin-coated structures appears to be a manifestation
175 Yet, in living cells, EH domains gathered at clathrin-coated structures are poorly accessible, indica
181 ficially dimerized construct of myosin VI on clathrin-coated structures suggests that wild type myosi
182 nse core vesicles) and endocytic structures (clathrin-coated structures) and the proteins associated
183 lathrin machinery, localizes to cell surface clathrin-coated structures, and is enriched in placental
187 teins are found to regulate the formation of clathrin coats under certain conditions, but can also su
189 (FCHo1/2) were required for plasma membrane clathrin-coated vesicle (CCV) budding and marked sites o
190 and then compared the protein composition of clathrin-coated vesicle (CCV) fractions from control and
198 P-2 cooperate to increase the probability of clathrin-coated vesicle formation and to control the num
199 thrin adaptor EPSIN1 (EPS1) is implicated in clathrin-coated vesicle formation at the trans-Golgi net
200 of adaptor protein-1 (AP1), responsible for clathrin-coated vesicle formation at the trans-Golgi, wa
206 roteins from HeLa cells and identified known clathrin-coated vesicle proteins with >90% accuracy.
207 iling is a universal method for defining the clathrin-coated vesicle proteome and may be adapted for
209 Thus, our results reveal the importance of clathrin-coated vesicle trafficking in C. burnetii infec
210 ovides novel insight into the TGN-associated clathrin-coated vesicle trafficking machinery that impac
211 athways include effector endocytosis through clathrin-coated vesicle trafficking, defense signaling t
213 e, auxilin, associates with a freshly budded clathrin-coated vesicle, or with an in vitro assembled c
216 ( approximately 300-nm diameter) and typical clathrin-coated vesicles ( approximately 90 nm) makes it
218 tests on subcellular fractions enriched for clathrin-coated vesicles (CCVs) indicated that pip5k1 an
222 as sorting signals for packaging cargo into clathrin-coated vesicles (CCVs), and also facilitate dow
223 ptor protein-2 (AP2), a central component of clathrin-coated vesicles (CCVs), is pivotal in clathrin-
224 on of the entire population of intracellular clathrin-coated vesicles (CCVs), suggesting a more globa
225 its of the adaptor protein (AP) complexes of clathrin-coated vesicles (CCVs), together with an FKBP a
226 ty, and leads to presynaptic accumulation of clathrin-coated vesicles (CCVs)-all without decreasing G
230 lattices and the transition to highly curved clathrin-coated vesicles - are adaptable and can follow
232 region that is required for localization to clathrin-coated vesicles and contains a putative pleckst
235 strate that REEP6 is detected in a subset of Clathrin-coated vesicles and interacts with the t-SNARE,
236 ody revealed that TBC1D24 is associated with clathrin-coated vesicles and synapses of hippocampal neu
238 ns were found to be associated with isolated clathrin-coated vesicles and to colocalize with clathrin
240 ex-2 (AP-2) is required for the formation of clathrin-coated vesicles at the plasma membrane (PM).
244 ndocytosis (CME) occurs via the formation of clathrin-coated vesicles from clathrin-coated pits (CCPs
253 al triskelia, suggesting that disassembly of clathrin-coated vesicles may proceed through a partially
256 We propose that AP180 directs Vamp7B into clathrin-coated vesicles on contractile vacuoles, creati
257 le vacuoles offer a valuable system to study clathrin-coated vesicles on internal organelles within e
259 ls is to link ubiquitinated Notch ligands to Clathrin-coated vesicles through other Clathrin adapter
260 2 may stimulate Gap1 incorporation into AP-1/clathrin-coated vesicles to promote Gap1 trafficking fro
261 EEP6 in trafficking of cargo via a subset of Clathrin-coated vesicles to selected membrane sites in r
262 SNAREs, required for the fusion of endocytic clathrin-coated vesicles with endosomes and also for sub
263 he plasma membrane, which invaginate to form clathrin-coated vesicles, a process that is well underst
264 o the adapter complex of the "inner" coat in clathrin-coated vesicles, and a heterotrimeric B-subcomp
265 ological structures, including mitochondria, clathrin-coated vesicles, and the actin cytoskeleton, in
266 kely determine the functional specificity of clathrin-coated vesicles, and together they control a mu
267 e endophilins results in the accumulation of clathrin-coated vesicles, but not of clathrin-coated pit
268 uce ATP7B incorporation into AP-1-containing clathrin-coated vesicles, caused loss of TGN localizatio
270 rin heavy chain (CHC), the main component of clathrin-coated vesicles, is well characterized for its
271 ted structures, and is enriched in placental clathrin-coated vesicles, new possibilities for Ced-6/Gu
272 are important components for the cleavage of clathrin-coated vesicles, phagosomes, and mitochondria.
274 and the late secretory route are mediated by clathrin-coated vesicles, while the COat Protein I and I