ライフサイエンスコーパス: claudin-5

1 otypically bind to claudin-1, claudin-3, and claudin-5.

2 n tight junction (TJ) proteins: occludin and claudin-5.

3 phosphorylation of occludin, ZO-1, ZO-2, and claudin-5.

4 ing zonula occludens-1 (ZO-1), occludin, and claudin-5.

5 fect of cARLA via the tight junction protein claudin-5.

6 dysfunction through modulation of junctional claudin-5.

7 art by tight junction (TJ) proteins, such as claudin-5.

8 as shown by immunostaining for occludin and claudin-5.

9 degradation of the TJ proteins occludin and claudin-5.

10 ; AII spectrin fragments, 1.9-fold increase; claudin-5, 2.7-fold increase; sodium-potassium-chloride

11 ctive diffusion of small molecules and makes claudin-5 a possible target for the development of drugs

12 In this issue, Nitta et al. demonstrate that claudin-5, a transmembrane protein of TJs, is a critical

13 Stroke decreases claudin-5 abundance, and we found that EC-selective miR-

14 esidues in the first extracellular domain of claudin-5 altered the properties of the tight junctions

15 Claudin-5, an endothelial tight-junction protein express

16 PA loss results in aberrant localization of Claudin 5 and -VE-Cadherin in endothelial cell junctions

17 Claudin 5 and eNOS Western blot analysis were used to me

18 n molecules zona occludens-1, claudin 3, and claudin 5 and other pathways critically involved in tran

19 HLEC(DeltaGATA2)) have altered expression of claudin 5 and VE-cadherin, and blocking miR-126 activity

20 ed expression of the tight junction proteins claudin 5 and ZO-1.

21 mice, as evidenced by tight junction protein Claudin-5 and adapter protein ZO-1 expression surroundin

22 ntine) rescued cerebrovascular expression of claudin-5 and blood-brain barrier permeability to both e

23 for the tight junction proteins occludin and claudin-5 and examined by confocal microscopy.

24 mpanied by decreased tight junction proteins Claudin-5 and glucose transporter Glut-1 in the brain en

25 well as tight junction proteins Occludin and Claudin-5 and increased barrier leakiness.

26 to a barrier-incompetent state with reduced Claudin-5 and increased PLVAP expression.

27 orting blood-brain barrier function, such as claudin-5 and intercellular adhesion molecule 1.

28 s in transendothelial electrical resistance, claudin-5 and occludin became internalized via caveolae

29 lation of cytoplasmic domains of recombinant claudin-5 and occludin by RhoK.

30 om a sucrose gradient showed the presence of claudin-5 and occludin in the same fractions that contai

31 However, total expression of claudin-5 and occludin remained unchanged except for a n

32 The tight junction proteins claudin-5 and occludin showed reduced surface expression

33 he amounts of major tight junction proteins, claudin-5 and occludin, in 12 brain regions dissected fr

34 A downregulation of TJ proteins, claudin-5 and occludin, paralleled monocyte migration in

35 ion of transferrin receptor, p-glycoprotein, claudin-5 and occludin.

36 ated BBB degradation by decrease of the TJPs claudin-5 and occludin.

37 ral endothelial cell tight-junction proteins claudin-5 and occludin.

38 levels of the tight junction proteins (TJPs) claudin-5 and occludin; increased expression of matrix-m

39 mportantly, the positive correlation between claudin-5 and synaptic markers, in particular synaptophy

40 cific interaction between the NVU/BBB marker claudin-5 and the inflammatory marker TNF-alpha in the p

41 rst to assess the relationship between serum claudin-5 and TNF-alpha levels, during major depressive

42 Serum levels of claudin-5 and TNF-alpha of 40 patients diagnosed with cu

43 Claudin-5 and TNF-alpha serum levels in the MDE group we

44 icant positive correlation was found between claudin-5 and TNF-alpha serum levels.

45 Claudin-5 and vascular endothelial-cadherin (VE-cadherin

46 ced in vivo permeability and dissociation of claudin-5 and VE-cadherin at junctional complexes.

47 EC expression paralleled decreased levels of claudin-5 and VE-PTP.

48 HIV-1-induced IL-6, diminished HIV-1-induced claudin-5 and ZO-1 down-regulation, and blocked HIV-1- a

49 gulation, exogenous glucocorticoids regulate claudin-5 and ZO-2 in vivo at some, but not all ages, an

50 meability was correlated with an increase in claudin-5 and zonula occludens-1 immunofluorescence at c

51 GCs also reduced levels of occludin, claudin 5, and caveolin 1, proteins central to blood-bra

52 Tight junction components ZO-1, claudin 5, and occludin were decreased at both the trans

53 (elevated levels of tight-junction protein, Claudin 5, and reduced S100B levels in periphery).

54 tial for GC-mediated expression of occludin, claudin-5, and barrier induction, and the p54/PSF hetero

55 ltiple junctional genes, including occludin, claudin-5, and cadherin-9.

56 ht junction protein down-regulation of ZO-1, claudin-5, and JAM-1 in HBMEC.

57 und to the 3' untranslated region (3'UTR) of Claudin-5, and lentivirus-mediated ablation of miR-15a/1

58 tight junction proteins (zonula occludens 1, claudin-5, and occludin), astrocyte activation, IgG extr

59 unctional phenotype as assessed by occludin, claudin-5, and ZO-1 immunoreactivities.

60 and protein expression of ZO-1, occludin and claudin-5 are prevented with inhibition of nociceptive i

61 akage, and elevated levels of syndecan-1 and claudin-5 are strongly associated with severe plasma lea

62 hosphorylated occludin at T382 and S507, and claudin-5 at T207 from full-length recombinant occludin

63 These new insights into the functions of claudin-5 at the molecular level in tight junctions may

64 chizophrenia in 22q11DS, leading to 75% less claudin-5 being expressed in endothelial cells.

65 e tight junction proteins ZO-1, occludin and claudin-5 between endothelial cells.

66 d the direct phosphorylation of occludin and claudin-5 by RhoK at specific sites, which was increased

67 bution of TJ proteins (occludin, ZO-1, ZO-2, claudin-5) by CCL2.

68 omplex reduced claudin-5 strands (homophilic claudin-5 cis- and trans-interactions) and claudin-5/ZO-

69 downregulation of the tight junction protein claudin 5 (CLDN5).

70 the endothelial cell tight junction protein claudin-5 (Cldn5) and abnormal blood vessel morphology i

71 ression of genes such as Keratin 20 (Krt20), Claudin-5 (Cldn5) and Nuclear Receptor Subfamily 4 Group

72 grity through loss of tight junction protein claudin-5 (cldn5) in male mice, promoting passage of cir

73 eability to smaller molecules, and decreased claudin-5 (CLDN5) levels.

74 are tightly coupled, express high levels of Claudin-5 (CLDN5), a junctional protein that stabilizes

75 Claudin-5 (CLDN5), an important component of tight junct

76 helial transmembrane tight junction proteins claudin-5 (CLN-5) and occludin (OCLN) as targets of VEGF

77 pression of S1PR1 and tight junction protein claudin-5, concomitantly with increased vascular permeab

78 ential endothelial TJ proteins, occludin and claudin-5, contribute and possibly disrupt BBB integrity

79 es including expression of GLUT-1, increased claudin-5, decreased PLVAP, and decreased permeability.

80 al-time RT-PCR assays for BCRP, occludin and claudin-5 demonstrated no significant differences betwee

81 Claudin-5 depletion only mimicked ZO-1 effects on barrie

82 Unexpectedly, knockout of claudin-5 did not result in a general breakdown of TJs b

83 Claudin-5 distribution was altered in small- to medium-s

84 vation of PPAR-gamma prevented HIV-1-induced claudin-5 down-regulation and significantly reduced vire

85 cription factor to trigger the expression of claudin-5 downstream of JAM-A, to thus enhance vascular

86 , gain-of-function for C/EBP-alpha increased claudin-5 expression and decreased endothelial permeabil

87 e HIV/AIDS models, we demonstrated decreased claudin-5 expression and increased macrophage infiltrati

88 sing siRNA by itself caused up-regulation of claudin-5 expression and partial protection from cytotox

89 paracellular pathway, demonstrated decreased claudin-5 expression at 24 h, and an increase at 48 and

90 ury and now we demonstrate a contribution of claudin-5 expression in IL-4-induced protection.

91 chotic medications dose-dependently increase claudin-5 expression in vitro and in vivo while aberrant

92 ha) was found to affect BBB permeability and claudin-5 expression of NVU endothelial cells.

93 Increased claudin-5 expression resulted in increased transmembrane

94 The increased claudin-5 expression was not limited to the junction.

95 gs, Efavirenz, but not other NNRTIs, altered claudin-5 expression, increased endothelial permeability

96 of the tight junctions formed in response to claudin-5 expression.

97 din for blood vessels, and downregulation in claudin-5 expression.

98 s necessary for GC induction of occludin and claudin-5 expression.

99 ein claudin-5, with concomitant reduction in claudin-5 expression.

100 ailing CMs displayed downregulated ephrin-B1/claudin-5 gene expression linearly related to the ejecti

101 Here, we show that a variant in the claudin-5 gene is weakly associated with schizophrenia i

102 population who are haploinsufficient for the claudin-5 gene.

103 ation and intracellular fate of occludin and claudin-5, green fluorescent protein fusion proteins of

104 association with the tight junction protein, claudin-5, has previously been identified.

105 eactivity and ConA binding, but no change in claudin-5 immunoreactivity was detected.

106 e expression of cleaved Notch-1, villin, and claudin 5 in colon samples from mice and humans.

107 ced expression of cleaved Notch, villin, and claudin 5 in colonocytes and significantly reduced the p

108 the cell junction molecules VE-cadherin and claudin 5 in lymphatic vessels.

109 The abcb4 probe also colocalized with claudin-5 in brain endothelial cells.

110 ng RGMa-induced downregulation of PDGF-B and Claudin-5 in endothelial cells, triggering BCB-disruptio

111 ch as facilitative glucose transporter 1 and claudin-5 in freshly isolated rat retinal capillaries.

112 tion of tight junctions, we expressed murine claudin-5 in Madin-Darby canine kidney II cells.

113 suppression, reinforcing the crucial role of claudin-5 in normal neurological function.

114 induces upregulation of the junction protein claudin-5 in porcine ECs through activation of Jak/STAT6

115 while aberrant, discontinuous expression of claudin-5 in the brains of schizophrenic patients post m

116 eno-associated virus-mediated suppression of claudin-5 in the mouse brain results in localized BBB di

117 rescued expression of tight junction protein claudin-5 in the prelimbic cortex (PLC) of female CIE-ED

118 esion molecule-1 (VCAM-1) and a reduction in claudin-5 in the retinal endothelium.

119 d with significantly decreased expression of claudin-5 in the vasculature of various tissues, includi

120 reveal that ZO1 clustering is independent of claudin-5 in vivo.

121 n of TJ transmembrane proteins (occludin and claudin-5) in increased permeability of the brain endoth

122 ns) and claudin-5/ZO-1 interaction affecting claudin-5 incorporation into the TJ complex.

123 s of occludin, E-cadherin, beta-catenin, and claudin-5 increased significantly, whereas no obvious ch

124 s, occludin, junction adhesion molecule, and claudin-5, induced by TNF-alpha in BCECs and consequentl

125 n the developmental context, total levels of claudin-5 inversely correlate with TJ functionality.

126 niformly expressed, claudin 4 is absent, and claudin 5 is only expressed in endothelial junctions.

127 Claudin-5 is a protein component of many endothelial tig

128 Claudin-5 is expressed in endothelial cells forming part

129 ve demonstrated that the expression level of claudin-5 is governed by the expression of VE-cadherin.

130 Claudin-5 is necessary and sufficient to diminish alveol

131 silon) phosphorylation, and up-regulation of claudin-5 is suppressed by PKCepsilon inhibitor peptide

132 ion showed the opposite effects of decreased claudin-5 levels and increased endothelial permeability.

133 r, no significant correlation was found with claudin-5 levels.

134 h norrin and VEGF were required for enriched claudin-5 localization at the tight junction.

135 tissues, we demonstrated down-regulation of claudin-5 (marker of pulmonary barrier integrity), down-

136 evealed sparse (occludin) vs. clustered (ZO1/claudin-5) molecular architecture.

137 deprivation (OGD)-induced down-regulation of claudin-5 mRNA and protein abundance and endothelial bar

138 EC-selective miR-15a/16-1 deletion enhanced claudin-5 mRNA and protein abundance in ischemic mouse b

139 t junctional proteins (claudin-3, claudin-4, claudin-5, occludin, and ZO-1) and adherent junctional p

140 mRNAs and protein for claudin-5, occludin, and zona occludens 2 were also redu

141 protein expression of collagen-IV, laminin, claudin-5, occludin, and zonula occludens protein 1 was

142 hanced presence of TJ proteins, occludin and claudin-5, on EVs.

143 detected in junctions of the duct epithelia, claudin 5 only in junctions of acinar cells, whereas cla

144 xpression, but not with occludin, claudin-1, claudin-5 or ZO-1 expression in ovine cerebral cortices.

145 can-1 (OR = 1.004; 95% CI = 1.000-1.008) and claudin-5 (OR = 1.038; 95% CI = 1.004-1.074) had an adju

146 trate that in response to alcohol, increased claudin-5 paradoxically accompanies an increase in parac

147 Critically, a claudin-5 peptide mimetic reverses the deleterious effec

148 rotected BBB integrity and reversed occludin/claudin-5 phosphorylation associated with monocyte migra

149 nase (RhoK) activation mediates occludin and claudin-5 phosphorylation resulting in diminished barrie

150 Claudin-5 protein expression was 69% and 73% higher (P<0

151 les, and only later to small molecules, with claudin-5 proteins arrangement compacting during this ma

152 Therefore, expression of claudin-5 selectively decreased the permeability to ions

153 o MDE type indicated significantly increased claudin-5 serum levels in BD but not in MDD patients, co

154 lk1 (activin receptor-like kinase 1), Cldn5 (claudin 5), Sox18 (SRY-box transcription factor 18), Rob

155 rance of claudin-1 in the TJ complex reduced claudin-5 strands (homophilic claudin-5 cis- and trans-i

156 ble 'knockdown' mouse model, we further link claudin-5 suppression with psychosis through a distinct

157 develop seizures and die after 3-4 weeks of claudin-5 suppression, reinforcing the crucial role of c

158 on occludin (T382 and S507) and one site on claudin-5 (T207).

159 indicators of vascular dysfunction, such as claudin-5, thrombospondins, platelet-derived growth fact

160 of either cysteine abolishted the ability of claudin-5 to increase transepithelial resistance, and mu

161 BP-alpha then directly binds the promoter of claudin-5 to thereby promote its transcription.

162 To test the contribution of claudin-5 to this barrier function of tight junctions, w

163 hrough EPAC to up-regulate the expression of claudin-5, to thus decrease endothelial permeability.

164 ld increase in transepithelial resistance in claudin-5 transductants and a reduction in conductance o

165 monosaccharides was significantly changed in claudin-5 transductants compared to controls.

166 07 from full-length recombinant occludin and claudin-5 transiently expressed in COS-7 cells and mouse

167 tion of the tight junction proteins ZO-1 and claudin-5, upregulation of the adhesion molecules VCAM-1

168 roteins ZO-1, JAM-2, Occludin, Claudin-3 and Claudin-5, using in vitro cultures of the primary brain

169 ey roles in vascular stabilization including claudin-5, vascular endothelial-protein tyrosine phospha

170 EC association increases Ezh2 recruitment to claudin-5, VE-PTP, and vWf promoters, causing gene downr

171 ve complex-2) binding to promoter regions of claudin-5, VE-PTP, and vWf.

172 such as DLL4 (Delta-like protein 4), CLDN5 (claudin-5), VWF (von Willebrand factor), and CDH5 (VE-ca

173 Expression of cleaved Notch-1, villin, or claudin 5 was not detected in RAG1(-/-) colonocytes; the

174 Claudin-5 was higher at 60% than 70% of gestation, and t

175 Claudin-5 was higher in dexamethasone than placebo-treat

176 ly, JAM-A-C/EBP-alpha-mediated regulation of claudin-5 was lost in blood vessels from tissue biopsies

177 reover, levels of the tight junction protein claudin-5 were increased with norrin and VEGF or with VE

178 ession and activation of STAT1 and decreased claudin-5 were observed in microvessels from autopsied b

179 dherin immunocytochemistry and expression of claudin-5, which were all unaltered by high glucose.

180 on proteins zona occludens-1, claudin-1, and claudin-5, which were significantly reduced after heme e

181 eins, zonula occluden-1 (ZO-1), occludin and claudin-5 with CIP.

182 the promoter for the tight junction protein claudin-5, with concomitant reduction in claudin-5 expre

183 bsence of tight junction proteins (occludin, claudin-5, ZO-1 and JAM-1) in the parenchymal blood vess

184 We conclude that claudin-1, claudin-5, ZO-1, and ZO-2 expression exhibit differentia

185 L-6 expression, and diminishes expression of claudin-5, ZO-1, and ZO-2 in HBMECs.

186 endothelial cells expressed the TJ proteins claudin-5, ZO-1, and ZO-2; HIV-1 decreased TJ proteins e

187 In wild-type CMs, ephrin-B1 interacted with claudin-5/ZO-1 complex at the lateral membrane, whereas

188 c claudin-5 cis- and trans-interactions) and claudin-5/ZO-1 interaction affecting claudin-5 incorpora

189 ession of tight junction proteins (occludin, claudin-5, zona occluden-1) and increase in the levels o

190 e protein expression of occludin, claudin-1, claudin-5, zonula occludens (ZO)-1, and ZO-2, and a TJ a

191 ion-associated proteins, including occludin, claudin-5, zonula occludens-1, junctional adhesion molec