ライフサイエンスコーパス: cleavage furrow

1 zation at sites of mechanical stress and the cleavage furrow.

2 itotic entry, and formation of a cytokinesis cleavage furrow.

3 ins that participate in the formation of the cleavage furrow.

4 of key proteins, including GpIb alpha to the cleavage furrow.

5 lophase spindle midzone, which specifies the cleavage furrow.

6 on of AMR contraction with ingression of the cleavage furrow.

7 (CPC) and centralspindlin that instructs the cleavage furrow.

8 ted insertion of basolateral membrane in the cleavage furrow.

9 hich actin and membrane are delivered to the cleavage furrow.

10 tment of FIP3 and recycling endosomes to the cleavage furrow.

11 n is required for complete ingression of the cleavage furrow.

12 t how this actin and membrane traffic to the cleavage furrow.

13 cytosed membrane is focally delivered to the cleavage furrow.

14 he interphase cortex but not the cytokinetic cleavage furrow.

15 of Kif12, DdINCENP fails to localize on the cleavage furrow.

16 , the rafts were clearly associated with the cleavage furrow.

17 orial cell cortex at the site of the nascent cleavage furrow.

18 tile ring and triggers the ingression of the cleavage furrow.

19 ed cytokinesis without any ingression of the cleavage furrow.

20 contractile ring, and the ingression of the cleavage furrow.

21 ole for one or more phosphoinositides at the cleavage furrow.

22 c cells, the majority of which had a visible cleavage furrow.

23 reconstruction of the plasma membrane in the cleavage furrow.

24 king, potentially important processes at the cleavage furrow.

25 g that drives the rapid ingression of a deep cleavage furrow.

26 rminant cortical domains are bisected by the cleavage furrow.

27 involved in the delivery of endosomes to the cleavage furrow.

28 and later concentrates in the region of the cleavage furrow.

29 equired for localization of myosin II to the cleavage furrow.

30 nesis until resolution of DNA trapped in the cleavage furrow.

31 ng from defects in contractile forces at the cleavage furrow.

32 ytes and express both NMIIA and NMIIB at the cleavage furrow.

33 adhesions (FAs) controls the symmetry of the cleavage furrow.

34 bed to impaired accumulation of actin in the cleavage furrow.

35 stitutively active RhoA induced NMIIA at the cleavage furrow.

36 tes with astral microtubules to position the cleavage furrow.

37 A and phospholipids to anchor anillin at the cleavage furrow.

38 hen constrict the contractile ring to form a cleavage furrow.

39 or the correct placement of the proteloblast cleavage furrow.

40 flow reverses direction toward the emerging cleavage furrow.

41 structions and the maintenance of the stable cleavage furrow.

42 nd ARX-2/Arp2 facilitates ingression of weak cleavage furrows.

43 ased to central spindle-based positioning of cleavage furrows.

44 ilizing and preventing retraction of nascent cleavage furrows.

45 bryo, the centrosome coordinates assembly of cleavage furrows.

46 ppeared to be caused by formation of ectopic cleavage furrows.

47 protein does not localize to the membrane or cleavage furrows.

48 rexpressed Buc-GFP, accumulates at embryonic cleavage furrows.

49 gether with other factors, organize into the cleavage furrow [1].

50 ted in fly spermatocytes that fail to form a cleavage furrow [3] and during monopolar cytokinesis whe

51 t cortical movements are concentrated in the cleavage furrow [8-13].

52 teins to their proper location, corrects the cleavage furrow abnormalities, and restores genomic stab

53 n showed that RMD1 is required for myosin II cleavage furrow accumulation, acting in parallel with me

54 in II to identify factors involved in myosin cleavage furrow accumulation.

55 sis-deficient mutant cell line devoid of the cleavage furrow actin bundling protein, cortexillin I.

56 s to keep sister genomes apart and guide the cleavage furrow after anaphase.

57 t can be activated if DNA is retained in the cleavage furrow after completion of anaphase.

58 ted cells caused by failed abscission of the cleavage furrow after telophase.

59 These vesicles are recruited to the cleavage furrow along the central spindle and a distinct

60 GpIb alpha and Aurora B redistribute to the cleavage furrow along with other cleavage furrow protein

61 es (metaphase and anaphase), spindle midzone/cleavage furrow (anaphase and telophase), and midbody (c

62 aphase and telophase, myosin II moves to the cleavage furrow and appears to "push" rather than carry

63 ning recycling endosomes accumulate near the cleavage furrow and are required for successful completi

64 n the delivery of recycling endosomes to the cleavage furrow and are, together with Rab11, essential

65 fficking and membrane remodeling to form the cleavage furrow and complete daughter cell separation.

66 feres with the localization of MyoGEF to the cleavage furrow and decreases MyoGEF activity toward Rho

67 body, a structure that anchors the ingressed cleavage furrow and guides the assembly of abscission ma

68 tokinesis, by allowing mispositioning of the cleavage furrow and inappropriate cytokinesis.

69 onclude that PtdIns(4,5)P2 is present at the cleavage furrow and is required for normal cytokinesis a

70 but also endocytic vesicle transport to the cleavage furrow and it does so through sequential intera

71 (ECM) remodeling is thought to stabilize the cleavage furrow and maintain cell shape during cytokines

72 It concentrates at the cleavage furrow and midbody during anaphase, telophase,

73 a multi-domain protein that localizes to the cleavage furrow and midbody of mitotic cells, where it i

74 The distribution of pPLK4 midzone/cleavage furrow and midbody positions pPLK4 to play a fu

75 umulation of RAB-11-positive vesicles at the cleavage furrow and midbody that is not a consequence of

76 ive vesicles into the plasma membrane at the cleavage furrow and midbody.

77 elophase FP-PP1gamma also accumulates at the cleavage furrow and midbody.

78 revents translocation to the spindle midzone/cleavage furrow and prevents cellular abscission, leadin

79 nd Rac1-GTP in epithelial cells--RhoA at the cleavage furrow and RhoA and Rac1 at cell-cell junctions

80 anous organelle, the fusome, grows along the cleavage furrow and the remnants of the mitotic spindle

81 senger proteins are known to localize on the cleavage furrow and to play a role in cytokinesis.

82 reover, Mkif5Ba is required to enrich Buc at cleavage furrows and for the ability of Buc to promote e

83 ically, Mkif5Ba is required to recruit GP to cleavage furrows and thereby specifies PGCs.

84 Sti recruits Nebbish to the cleavage furrow, and both proteins are required for midb

85 MYH10 [NMIIB]), only NMIIB localizes at the cleavage furrow, and its subsequent absence contributes

86 to key cytokinetic structures including the cleavage furrow, and later, the abscission site.

87 RhoA and active myosin were absent from the cleavage furrow, and myosin was redistributed to cortica

88 dissolution of the midbody, reopening of the cleavage furrow, and realignment of chromosomes at the m

89 , becomes associated with the unipolar first cleavage furrow, and remains enriched in animal pole bla

90 is recruited to the adherens junction at the cleavage furrow, and that inhibiting recruitment of Vinc

91 d dynactin participate in stimulation of the cleavage furrow, and their interaction promotes elongati

92 are required to maintain the position of the cleavage furrow, and these signals control the localizat

93 hown an association of microtubules with the cleavage furrow, and we used a fluorescently tagged EB1

94 f filamentous fungi and dendritic spines, in cleavage furrows, and in retracting membrane protrusions

95 umulation and phospholipid metabolism at the cleavage furrow are unchanged in cells expressing ARF6 m

96 nd separation, and it accumulated within the cleavage furrow around the spindle midzone.

97 a molecular link between the centrosome and cleavage furrow assembly.

98 ge furrows in early embryos, is required for cleavage furrow assembly.

99 CM2, blocks the binding of CEN and disrupts cleavage furrow assembly.

100 r within a domain highly homologous to yeast cleavage furrow-associated protein CDC15.

101 ance of maintaining a delicate balance among cleavage furrow-associated proteins during mitosis.

102 ts, suggesting that TOE-2 might position the cleavage furrow asymmetrically to generate daughter cell

103 sion, chromosomes must clear the path of the cleavage furrow before the onset of cytokinesis.

104 ential for Arf1 to function at the Golgi for cleavage furrow biosynthesis.

105 ndergo symmetric cell divisions in which the cleavage furrow bisects the apical domain.

106 filaments is necessary for targeting to the cleavage furrow but not to the interphase cortex.

107 rens junction proteins are stabilized at the cleavage furrow by increased tension.

108 ilament assembly, fails to accumulate at the cleavage furrow, cannot rescue myoII-null cytokinesis, a

109 Because citron kinase is localized in cleavage furrows, citron kinase may be involved in regul

110 ion errors, defective assembly of actin into cleavage furrows, cleavage failure, a rise in cyclin E l

111 t of cell contacts is to position the D cell cleavage furrow closer to the animal pole, and the enhan

112 Here, we report that, after cleavage furrow closure, septins form a membrane-bound d

113 an important role for Anillin in scaffolding cleavage furrow components, directly stabilizing intrace

114 ase, and was maximally activated just before cleavage furrow constriction.

115 n, and controls the symmetry and kinetics of cleavage furrow contractility during cytokinesis.

116 uding spindle assembly, spindle positioning, cleavage furrow contraction and abscission.

117 surface protrusions, it is excluded from the cleavage furrow cortex during cytokinesis.

118 tin crosslinkers have slower kinetics in the cleavage furrow cortex than in the pole, that their kine

119 ntractile ring and subsequent formation of a cleavage furrow, DdINCENP is important for the abscissio

120 ono- and di-phosphorylated MLC were found in cleavage furrows, di-phosphorylated MLC showed more cons

121 At cellularization, however, cleavage furrows do not invaginate between nuclei and co

122 l culture localized to the cell membrane and cleavage furrow during cell division as well as in the n

123 receptor potential channel, localizes to the cleavage furrow during cytokinesis of the fission yeast,

124 90 results in decreased RhoGTP levels at the cleavage furrow during cytokinesis, p190 and the RhoGEF

125 er a molecular mechanism for positioning the cleavage furrow during cytokinesis.

126 cells, but both accumulated together at the cleavage furrow during cytokinesis.

127 if12 is found on the central spindle and the cleavage furrow during cytokinesis.

128 le midzone, and poles during mitosis and the cleavage furrow during cytokinesis.

129 olarize; additionally, it accumulates in the cleavage furrow during cytokinesis.

130 reakdown at mitosis, and concentrates at the cleavage furrow during embryonic cell division.

131 The geometry of the cleavage furrow during mitosis is often asymmetric in vi

132 r chromosomal structure, centrosome, and the cleavage furrow during prophase through telophase.

133 tes during mitosis and concentrates near the cleavage furrow during telophase.

134 ne that normally develops at the apex of the cleavage furrow during the final phase of cytokinesis do

135 pseudocleavage furrows, and to somatic cell cleavage furrows during the postcellularization embryoni

136 hese mutant cells initiate the ingression of cleavage furrows earlier than normal, shortening the sta

137 In the absence of hemicentin, cleavage furrows form but retract prior to completion, r

138 r lobulation, suggesting a relationship with cleavage furrow formation and abortive cytokinesis.

139 ion to the equatorial cortex, and suppresses cleavage furrow formation and cell division.

140 The central spindle regulates cleavage furrow formation and cytokinesis and is compose

141 retardation protein (dFMRP) is required for cleavage furrow formation and functions within dynamic c

142 tor of the small GTPase RhoA, which controls cleavage furrow formation and ingression.

143 etaphase-anaphase transition, Plk1 initiates cleavage furrow formation and is then progressively degr

144 t modulate the actin cytoskeleton to promote cleavage furrow formation and progression during cytokin

145 t regulation of CCT subunits is required for cleavage furrow formation and that at least one of its s

146 RhoA controls cleavage furrow formation during cell division, but whet

147 nd this role is also likely to be crucial in cleavage furrow formation during cell division.

148 f Ect2 are essential for RhoA activation and cleavage furrow formation in human cells.

149 y identified protein Centrocortin to promote cleavage furrow formation in the early embryo.

150 Cleavage furrow formation marks the onset of cell divisi

151 y RNA interference, 58% displayed defects in cleavage furrow formation or completion, or germline cyt

152 pendent regulation of CCT contributes to the cleavage furrow formation phenotype.

153 show that the C terminus of CNN coordinates cleavage furrow formation through binding to CEN, thereb

154 on that occurs after chromosome segregation, cleavage furrow formation, and contraction have complete

155 RP-dependent regulation and new effectors of cleavage furrow formation, we used two-dimensional diffe

156 MRP (dFMRP) is required in early embryos for cleavage furrow formation.

157 genetically that tral itself is required for cleavage furrow formation.

158 ) leads to defects earlier in M phase before cleavage furrow formation.

159 pindlin is essential for central spindle and cleavage furrow formation.

160 We conclude that the two pathways of cleavage-furrow formation are mechanistically distinct.

161 It is widely believed that cleavage-furrow formation during cytokinesis is driven b

162 lar, we conclude that the astral pathway for cleavage-furrow formation involves the negative regulati

163 inesis into two separate daughter cells by a cleavage furrow formed by the actomyosin-based contracti

164 le ring but during septation appeared in the cleavage furrow, forming a washer or disc structure.

165 d to maintain myosin II in the cortex of the cleavage furrow, generating unseparated daughter cells c

166 e present or of functional importance at the cleavage furrow has not been published.

167 ipolar mitosis followed by regression of the cleavage furrow hours later.

168 e, targeted membrane secretion occurs at the cleavage furrow in animal cells, and proteins that regul

169 g generates force to ingress the cytokinetic cleavage furrow in animal cells, yet its filament organi

170 Cytokinesis partitions the cell by a cleavage furrow in animals but by a new cross wall in pl

171 -cell contacts also direct ingression of the cleavage furrow in coordination with FAs in epithelial c

172 How myosin II localizes to the cleavage furrow in Dictyostelium and metazoan cells rema

173 oglycan chondroitin in the completion of the cleavage furrow in dividing Caenorhabditis elegans embry

174 mental biological problem-positioning of the cleavage furrow in dividing cells-to explain how and why

175 a protein that is highly concentrated in the cleavage furrow in numerous animal cells in a pattern th

176 in which LET-99 localizes to the presumptive cleavage furrow in response to the spindle and myosin.

177 checkpoint in mammalian cells stabilizes the cleavage furrow in the presence of a chromatin obstructi

178 do not polymerize in vitro do not go to the cleavage furrow in vivo.

179 The cleavage furrow in Xenopus zygotes is positioned by two

180 ch associates with centrosomes and also with cleavage furrows in early embryos, is required for cleav

181 otubules (MTs) are known to be important for cleavage furrow induction and spindle positioning, and l

182 tuent of the spindle apparatus essential for cleavage furrow induction.

183 ns embryos causes late cytokinesis failures: cleavage furrows ingress but subsequently regress and th

184 In anillin knockdown cells, the cleavage furrow ingressed but failed to complete the ing

185 ottom (i.e., substrate attached side) of the cleavage furrow ingressed less than the top (i.e., unatt

186 kinesis, there is a lengthy interval between cleavage furrow ingression and abscission, during which

187 ence supporting a novel role for ARF6 during cleavage furrow ingression and cytokinesis.

188 scovered calcium spikes both at the start of cleavage furrow ingression and the end of cell separatio

189 the postmitotic cells, resulting in partial cleavage furrow ingression during cytokinesis.

190 -function mutations in fws caused failure of cleavage furrow ingression in dividing spermatocytes and

191 t the conserved Arf GAP Asap is required for cleavage furrow ingression in the early embryo.

192 cence polarization microscopy and found that cleavage furrow ingression initiates by contraction of a

193 Remodeling of the plasma membrane during cleavage furrow ingression involves the exocytic and end

194 the fission yeast Schizosaccharomyces pombe, cleavage furrow ingression is driven by polymerization o

195 ic event in Drosophila embryos, to show that cleavage furrow ingression is kinetically coupled to the

196 Furthermore, cleavage furrow ingression is sensitive to the balance o

197 hiphysin-null embryos correlates with faster cleavage furrow ingression rates.

198 tubules formed, which correlated with slower cleavage furrow ingression rates.

199 ransiently at the division plane just before cleavage furrow ingression, suggesting that these calciu

200 ive cell imaging, we have found that, during cleavage furrow ingression, vesicles are internalized fr

201 coordinate sister chromatid segregation with cleavage furrow ingression.

202 h the small molecule PPMP, causes failure of cleavage furrow ingression.

203 these pathways converge to ensure successful cleavage furrow ingression.

204 regulate actomyosin ring contraction during cleavage furrow ingression.

205 s, a reservoir of microvilli unfolds to fuel cleavage furrow ingression.

206 existence of two pathways that can initiate cleavage furrow ingression.

207 ctomyosin ring, but its role, if any, during cleavage-furrow ingression and abscission is poorly unde

208 hain fatty acids, block or dramatically slow cleavage-furrow ingression during early telophase in div

209 negative Rho1 inhibited SS formation but not cleavage-furrow ingression or the concomitant actomyosin

210 S formation), while simultaneously promoting cleavage-furrow ingression through primary septum format

211 over, Rho1 is temporarily inactivated during cleavage-furrow ingression; this inactivation requires t

212 med cell membranes from cytokinesis, whereas cleavage furrow initiation, mitotic spindle function, ka

213 or that localizes to the central spindle and cleavage furrow, interacts with centrosome/spindle pole-

214 By manipulating the cleavage furrow into different shapes, we show that the

215 f cellularization, leading to the failure of cleavage furrow invagination and the generation of multi

216 During cytokinesis, cleavage furrow invagination requires an actomyosin-base

217 ium INCENP (DdINCENP) with the cortex of the cleavage furrow involves interactions with the actin cyt

218 The inactivation of Rac1 at the cleavage furrow is controlled by MgcRacGAP.

219 Formation of the mitotic cleavage furrow is dependent upon both microtubules and

220 how that the localization of DdINCENP at the cleavage furrow is modulated by myosin II but it occurs

221 ortex after anaphase onset but that a normal cleavage furrow is not induced until contact with MTs ha

222 During animal cell division, the cleavage furrow is positioned by microtubules that signa

223 Anillin accumulation at the cleavage furrow is Rho guanine nucleotide exchange facto

224 Myosin II localization to the cleavage furrow is severely depressed in Kif12-null (Del

225 actile-ring constriction, but the rim of the cleavage furrow is the main site for endocytosis.

226 st that chromatin trapped in the cytokinetic cleavage furrow is the more likely reason for furrow reg

227 The cytokinetic cleavage furrow is typically positioned symmetrically re

228 n, an actin-binding protein localized at the cleavage furrow, is required for cytokinesis.

229 sma membrane, we observed the recruitment of cleavage furrow markers, including an active RhoA report

230 ular matrix may be a general requirement for cleavage furrow maturation and contractile ring function

231 This spindle-independent cleavage furrow mechanism may be relevant to other highl

232 icle traffic from recycling endosomes to the cleavage furrow/midbody where they are tethered prior to

233 the adherens junctions by a parallel mitotic cleavage furrow, most re-establish contact with the apic

234 le into a discrete ring in the Dictyostelium cleavage furrow of adherent cells.

235 s of Acanthamoeba myosin II also goes to the cleavage furrow of Dictyostelium myosin II-null cells.

236 o colocalize with this myosin isoform in the cleavage furrow of dividing cells by indirect immunofluo

237 at extracellular hemicentins assemble at the cleavage furrow of dividing cells in the C. elegans germ

238 How myosin II localizes to the cleavage furrow of dividing cells is largely unknown.

239 ell cortex, lamellar actin networks, and the cleavage furrow of dividing cells--always together with

240 the endoplasmic reticulum, mitochondrion, or cleavage furrow of dividing cells.

241 in myosin II-null cells and localizes to the cleavage furrow of dividing cells.

242 calized to the newly forming and contracting cleavage furrow of dividing cells.

243 e, the leading edge of motile cells, and the cleavage furrow of dividing cells.

244 e polarity protein Pard3 is localized to the cleavage furrow of dividing progenitors, and then mirror

245 time scale, including rapid migration to the cleavage furrow of mitotic cells.

246 t the plasma membrane and accumulated in the cleavage furrow of the Q.a and Q.p neuroblasts, suggesti

247 her microcephalic mouse mutants, mitosis and cleavage furrows of cortical stem cells appear normal in

248 RhoA was localized at the cleavage furrow or at the necks of blebs.

249 hypothesis that the exquisite control of the cleavage furrow orientation in mammalian neural precurso

250 e asymmetry are key determinants for correct cleavage furrow placement and cortical expansion, thereb

251 and triplet microtubules and have defects in cleavage furrow placement and meiosis.

252 ucleus positioning, but resulted in aberrant cleavage furrow placement and the generation of non-equi

253 y of a robust central spindle to specify the cleavage furrow position, to prevent separated chromosom

254 unequal cortical expansion, ensuring correct cleavage furrow positioning and the establishment of phy

255 Microtubules (MTs) are essential for cleavage furrow positioning during cytokinesis, but the

256 identify a spindle-independent mechanism for cleavage furrow positioning in Drosophila neuroblasts.

257 studies suggest that membrane domains at the cleavage furrow possess a signaling pathway that contrib

258 osophila cleavage, myosin recruitment to the cleavage furrows proceeds in temporally distinct phases

259 nd physically interacts with three conserved cleavage furrow proteins, F-actin, myosin II and septins

260 bute to the cleavage furrow along with other cleavage furrow proteins.

261 perpendicular to two basal domains and their cleavage furrow rarely bifurcates the luminal domains.

262 nisms as the assembly domain is required for cleavage furrow recruitment of a truncated coiled-coil t

263 g spindle morphology and kif12 and myosin II cleavage furrow recruitment.

264 2b-deficient cells attempt anaphase, but the cleavage furrow regresses and cytokinesis fails.

265 s time to resolve the obstruction before the cleavage furrow regresses or breaks the chromosomes, pre

266 r collapses to one side of the cell, and the cleavage furrow regresses.

267 lear size and morphology, chromatin bridges, cleavage furrow regression, and tetraploidy.

268 d with subtle Golgi reorganization preceding cleavage furrow regression.

269 ed in which an ectopic myosin cap shifts the cleavage furrow relative to the spindle center.

270 icles with new membrane are deposited to the cleavage furrow relatively evenly during contractile-rin

271 The mitotic spindle determines the cleavage furrow site during metazoan cell division, but

272 I generates regional mechanics by increasing cleavage furrow stiffness and slows furrow ingression du

273 furrows that are structurally related to the cleavage furrows that assemble during somatic cell divis

274 nents in diverse morphogenetic events in the cleavage furrow, the germline, and neurons.

275 control assembly of two alternative forms of cleavage furrows: the 'Rappaport' furrows, which govern

276 division, N-cadherin is enriched in the post-cleavage furrow; then one cell pivots around the other,

277 s organelle lies directly in the path of the cleavage furrow, this delay may explain, at least in par

278 RhoA-regulated actomyosin contraction of the cleavage furrow; this process is aborted in megakaryocyt

279 ivity that impairs NMIIA localization at the cleavage furrow through increased actin turnover.

280 The cleavage-furrow tip adjacent to the actomyosin contracti

281 ys, including endocytic tubules that form at cleavage furrow tips (CFT-tubules).

282 This leads either to failure of the cleavage furrow to form or later defects in which daught

283 he neck of cellular appendages and/or at the cleavage furrow to help compartmentalize the plasma memb

284 reaction and has been proposed to attach the cleavage furrow to the intercellular bridge.

285 ll during anaphase, effectively shifting the cleavage furrow toward the smaller daughter cell.

286 We show that Myosin relocalizes to the cleavage furrow via two distinct cortical Myosin flows:

287 3xAsp myosin II's localization to the cleavage furrow was rescued by constructs encoding rcdBB

288 s undergoing division, thylakoids inside the cleavage furrow were kinked and severed.

289 In anaphase, p120 is enriched at the cleavage furrow where it binds MKLP1 to spatially contro

290 lines revealed asymmetry in the shape of the cleavage furrow, where the bottom (i.e., substrate attac

291 the peripheral ends of the first and second cleavage furrows, where they form part of the zebrafish

292 to the interphase cortex or the cytokinetic cleavage furrow, whereas a 500-residue region (1350-1865

293 filament reorientation along the cytokinetic cleavage furrow, which might have implications for diver

294 tes the actomyosin-driven contraction of the cleavage furrow, which proceeds until the formation of a

295 g to failure to inhibit formation of ectopic cleavage furrows, which result in mitotic defects and DN

296 cles or tubulovesicular structures along the cleavage furrow while the exocyst tethers vesicles at th

297 the region of the plasma membrane where the cleavage furrow will assemble is a classic question in c

298 1 and Ect2 in regulating Rho activity in the cleavage furrow, with GEF-H1 catalyzing Rho activation i

299 bbistatin, that inhibited contraction of the cleavage furrow without disrupting mitosis or contractil

300 and F-actin are familiar cohabitants of the cleavage furrow yet how they might be functionally conne