ライフサイエンスコーパス: click chemistry

1 atalyzed Huisgen 1,3-dipolar cycloaddition ("click chemistry").

2 ions, has been synthesized via alkyne-azide "click chemistry".

3 cyclic alkyne reagents for fast and tunable "click chemistry".

4 quipped with a propargylglycine residue for "click chemistry".

5 ed azide-alkyne cycloaddition, often termed "click chemistry".

6 ymerization (ATRP) signal amplification and "Click Chemistry".

7 oselective fluorescence tagging by means of 'click chemistry'.

8 synthesized proteins were then labeled via 'click chemistry'.

9 dibenzocyclooctyne (DBCO)-Cy5 by subsequent click chemistry.

10 cently emerging applications in the field of click chemistry.

11 -selective peptide cyclo(FRGDLAFp(NMe)K) via click chemistry.

12 und to SH functionalities applying thiol-ene click chemistry.

13 ygen-sensing dendrimers through azide-alkyne click chemistry.

14 onversion to the corresponding triazoles via click chemistry.

15 nm in length were grown layer-by-layer using click chemistry.

16 dipolar cycloaddition reaction, a variant of click chemistry.

17 none for photocrosslinking and an alkyne for CLICK chemistry.

18 containing cellular proteins by azide-alkyne click chemistry.

19 owing since its stimulation by the advent of click chemistry.

20 d to azide functionalized VHH domains, using click chemistry.

21 oxynaphthalene (DNP) recognition units using click chemistry.

22 hus going far beyond the original purpose of click chemistry.

23 l PG using d-amino acid dipeptide probes and click chemistry.

24 ed exploiting 5'-ethynyluridine labeling and click chemistry.

25 like receptors (TLRs) and also determined by click chemistry.

26 synthesized by convergent methodology using click chemistry.

27 s novel clubbed 1,2,3-triazole hybrids using click chemistry.

28 vator, 17-alpha-ethynylestradiol (17EE), via click chemistry.

29 ns for the use and applications of thiol-ene click chemistry.

30 labeled with mass tags to the bound probe by Click chemistry.

31 amine and pyrene azide using Cu(I)-catalyzed click chemistry.

32 ional design of new reagents for copper-free click chemistry.

33 high-relaxivity multiplexed probes utilizing click chemistry.

34 ring-expansion metathesis polymerization and click chemistry.

35 tabolically labeled glycans with copper-free click chemistry.

36 h intrinsic reactivities desired for Cu-free click chemistry.

37 immobilization of azides using catalyst-free click chemistry.

38 uran salicylic acid derivatives assembled by click chemistry.

39 tach different, large hydrophobic groups via click chemistry.

40 ng and tracking strategy using bioorthogonal click chemistry.

41 matic method using the engineered enzyme and click chemistry.

42 merization modification with triazolinedione click chemistry.

43 (SuFEx) has emerged as the new generation of click chemistry.

44 proteins with a non-canonical amino-acid and click chemistry.

45 hen assembled in a combinatorial fashion via click chemistry.

46 king such functionality available for use in click chemistry.

47 ementary oligonucleotides, and bioorthogonal click chemistry.

48 ovalent cross-linking using copper catalyzed click chemistry.

49 re conjugated to IRDye 800 using copper-free click chemistry.

50 oinitiators, which makes it truly two-photon click chemistry.

51 -selective peptide cyclo(FRGDLAFp(NMe)K) via click chemistry.

52 erns and directly on microelectrodes with UV-click-chemistry.

53 orophores via bioorthogonal copper-catalyzed click-chemistry.

54 red to the surface utilizing bio-orthogonal "click" chemistry.

55 yzed azide-alkyne cycloaddition reaction in "click" chemistry.

56 sized using stepwise imine condensation, or "click" chemistry.

57 rivatized conducting PEDOT electrodes using "click" chemistry.

58 incorporation of unnatural amino acids and "click" chemistry.

59 Ru(II) polypyridyl chromophores coupled via "click" chemistry.

60 because 1,2,3-TRZs are easily obtained via "click" chemistry.

61 diated amine-to-nitrile addition, a form of "click" chemistry.

62 hod using DNA methyltransferase (Mtase) and "click" chemistry.

63 receptor (AVP V(2)R) was synthesized using "Click" chemistry.

64 ole and triazole derivatives prepared using "click" chemistry.

65 tion of alkyne-derived motifs for so-called "click" chemistry.

66 with an azide-containing fluorophore using 'click' chemistry.

67 e being discussed, involving orthogonal and 'click' chemistries.

68 rged precursors, causing them to combine by "click" chemistry 1,000,000 times faster than without acc

69 he Fe(III)-HOPO ICP particles by copper-free click chemistry afforded colloidally stable nucleic-acid

70 Using click chemistry, Alexa Fluor 647 DIBO Alkyne was conjuga

71 The orthogonality of oNQM-thiol and azide click chemistry allowed for the development of a sequent

72 nt labels and - thanks to recent advances in click chemistry - an incredible versatility.

73 rporated an alkyne-containing amino acid for click chemistry, an important post-translationally modif

74 Click chemistry analyses demonstrated increased R-Ras pa

75 Ras was validated as an APT-1 substrate, and click chemistry analyses demonstrated increased R-Ras pa

76 tic acid into cellular proteins coupled with click chemistry and (ii) detecting a specific protein of

77 Using click chemistry and a modified antibody array to detect

78 proteins, allowing for their detection using click chemistry and antibody arrays.

79 lly conserved compounds that are amenable to click chemistry and can be used as molecular probes in v

80 of sulfur electrophiles is key for advancing click chemistry and chemical probe discovery.

81 igh sensitivity and spatial resolution using click chemistry and fluorescence microscopy.

82 minescent quantum dot (QD) surfaces that use click chemistry and hydrazone ligation under catalyst-fr

83 We adapted newly developed procedures for click chemistry and iPOND (Isolation of proteins on nasc

84 ace N-glycoproteins by combining copper-free click chemistry and MS-based proteomics.

85 Herein, an introduction to the impact of click chemistry and other bioorthogonal reactions on the

86 2,3-triazole moieties were prepared by using click chemistry and showed low nanomolar inhibitory acti

87 backbone using for the first time sequential click chemistry and stepwise assembly of functional buil

88 lyl donor/acceptor unnatural nucleosides via click chemistry and studies on the duplex stabilization

89 njugated with two oligoguanosine strands via click chemistry and the conjugates were then self-assemb

90 , we present an optimized strategy combining click chemistry and the genetic encoding of unnatural am

91 ibrary of these compounds was prepared using click chemistry and the selectivity concept was validate

92 the utility and multimodal possibilities of click chemistry and to increase enzyme active site inclu

93 ique combination of facile accessibility via click chemistry and truly diverse supramolecular interac

94 Whole cells are labelled via click chemistry and visualized using super-resolution mi

95 ole-based IL moieties were synthesized using click chemistry and were further copolymerized with laur

96 triazole linker is afforded by azide-alkyne "click" chemistry and comprises the third arm of the trip

97 eudomonas aeruginosa were synthesized using "Click" chemistry and DNA chemistry.

98 Here, combining metabolic labeling, click-chemistry and enzymatic reactions, and mass spectr

99 ates from the stoppering reaction, based on "click" chemistry, and thus from the presence of two tria

100 any different biological purposes, including click chemistry applications, diversity-oriented synthes

101 The oligoproline/click chemistry approach holds great promise for the pre

102 4) and sodium ascorbate independently of the click chemistry approach used (azido-COUPY/alkynyl-pepti

103 Hence, the click chemistry approach with an in situ template of a r

104 Herein, we report for the first time a "click" chemistry approach to oligonucleotide probe elong

105 taining azide (-N3) functionalities using a "click" chemistry approach.

106 Herein, we describe a "click chemistry" approach for the synthesis of PROTACs.

107 These studies establish Cu-free click chemistry as a bioorthogonal reaction that can be

108 inal alkyne group, we were able to visualize click chemistry at the single-molecule level, which reve

109 Among the dipoles routinely used for click chemistry, azides, nitrones, isonitriles, and nitr

110 The click chemistry based on the addition of photochemically

111 Here, we have used a click chemistry based strategy to introduce six pairs of

112 fluoride exchange (SuFEx) is a new family of click chemistry based transformations that enable the sy

113 We recently reported a click-chemistry based method for generating RNAseq libra

114 eloped a robust, high-throughput compatible, click chemistry-based approach to identify small molecul

115 Here we describe a click chemistry-based approach, using an azido derivativ

116 transformation into acyl-CoAs and subsequent click chemistry-based detection, to demonstrate that zDH

117 axolotl salamander tissue using whole-mount click chemistry-based fluorescent staining followed by l

118 A click chemistry-based flux assay reveals that necrocytos

119 sing a combination of metabolic labeling and click chemistry-based mass tagging.

120 g precursor was synthesized and subjected to click chemistry-based radiosynthesis of [(18)F]FGlc-FAPI

121 This letter presents a click-chemistry-based assay for proteins (CAP) that allo

122 Here we develop a click-chemistry-based enrichment strategy, DIMEN (deep i

123 sulfonates can be further functionalized via click-chemistry-based post-polymerization modification.

124 ith the ART probe can then be isolated using click chemistry before identification by liquid chromato

125 dy against respiratory syncytial virus using click chemistry between biotin molecules functionalized

126 METHODS AND Using crosslinking analogs, click chemistry, binding assays, and functional assays,

127 osecond photochemical reaction (nsPCR)-based click chemistry, capable of structural probing of protei

128 ecial focus capitalizes on the impact of the click chemistry concept on dendrimer synthesis and the p

129 ys a series of steps that capture fully the "click" chemistry concepts that have greatly facilitated

130 protein targets and has an alkyne handle for click chemistry conjugation to reporter tags.

131 Click chemistry, copper(I)-catalyzed azide-alkyne cycloa

132 Assisted with click-chemistry coupling, we sandwiched one G-quadruplex

133 (68)Ga-aquibeprin was obtained by click-chemistry (CuAAC) trimerization of a alpha5beta1 i

134 techniques, including acyl-biotin exchange, click chemistry, cysteine mutagenesis, and mass spectrom

135 ) linker was found compatible with different click chemistries, demonstrated in bioreversible protein

136 Click chemistry describes a family of modular, efficient

137 oionics an attractive opportunity for future click chemistry development.

138 orthogonal amino acid using copper-catalyzed click chemistry, either before or after the silk fibers

139 gamma-azido vinyl sulfones, and azide-alkyne click chemistry enabled the synthesis of vinyl sulfone-b

140 s as a cellular engagement probe for MPC2 in click chemistry-enabled western blotting or global mass

141 Click chemistry enables conjugation of an azide molecule

142 click reaction is orthogonal to azide-alkyne click chemistry, enabling sequential photoclick/azide-cl

143 Bioorthogonal click chemistry ensures fast and highly selective attach

144 bstitutes for high-throughput screening, and click chemistry ensures that chemical synthesis is fast,

145 We specifically focus on click chemistry, enzymatic ligation, and affinity bindin

146 Biotin conjugation to EdU-labeled DNA using click chemistry facilitates a single-step streptavidin p

147 various thiols by radical mediated thiol-ene click chemistry, followed by self-assembly in deionized

148 or D-glucose to L-cysteine using thiol-ene "click" chemistry, followed by their conversion to the co

149 al reagents that enable protein labeling via click chemistry for affinity purification and detection

150 derived components and relied on copper-free click chemistry for bioorthogonal covalent cross-links t

151 roach is highly versatile, as it (i) enlists click chemistry for flexible protein functionalization;

152 nation atoms were prepared in high yield via click chemistry for potential incorporation into metal c

153 K) that harnesses the power of bioorthogonal click chemistry for remodeling guide RNA to display synt

154 We report a new type of azide-alkyne click chemistry for the synthesis of protein conjugates

155 of biomolecules via Huisgen cycloaddition ("click chemistry") for positron emission tomography (PET)

156 hat can undergo bio-orthogonal ligation via 'click' chemistry, for example, an azide, and can be used

157 des and cyclooctynes, also known as "Cu-free click chemistry," for labeling biomolecules in live mice

158 mizing reaction conditions, we converted CB6 click chemistry from a rotaxane synthesis tool into a us

159 itterionic salts, which are prepared through click chemistry from readily accessible alkynes and sulf

160 ino acid side chains, including non-natural 'click' chemistry functionalities, to conjugate moieties

161 ification of target proteins, an alkyne as a click-chemistry handle for target identification, and a

162 Click chemistry has become a ubiquitous chemical tool wi

163 D chelator capable of direct conjugation via click chemistry has been reported.

164 and kinetics of radical initiated thiol-ene click chemistry has been studied computationally at the

165 labeling using sugar analogs compatible with click chemistry has the potential to provide new insight

166 the most versatile bioorthogonal reactions, "click chemistry", has been exploited to overcome limitat

167 Several forms of click chemistry have previously been used to modify poly

168 Assisted by click chemistry, herein, we attached pulling handles via

169 eling of target proteins and biologics using click chemistries, (ii) for glycoengineering of antibodi

170 al amino acid incorporation and cell surface click chemistry in bacteria with high-throughput sorting

171 trate the potential of SOF(4) -derived SuFEx click chemistry in biological applications.

172 tocol and the labeling of DNA-Pt by means of click chemistry in cells.

173 he successful advancement of Cu(I)-catalyzed click chemistry in glycoscience and its applications as

174 and evaluated a pretargeting strategy using click chemistry in vivo to reduce kidney uptake and avoi

175 combination of genetics and maleimide-based click chemistry in which a cysteine substitution is made

176 meter and synthesized via an astoichiometric click-chemistry in-emulsion method, controllably display

177 in one case and through a triazole linkage ('click chemistry') in the other.

178 les for sulfur(VI) fluoride exchange (SuFEx) click chemistry, in which either the alkenyl moiety or t

179 nality onto solid surfaces using well-known "click" chemistry involving easy-to-react linkers.

180 labeling followed by the use of reagent-free click chemistry is an established technique for in vitro

181 Target-guided synthesis by in situ click chemistry is combined with synthetic epitope targe

182 The use of thiol-ene click chemistry is demonstrated for the first time as a

183 ds and their subsequent macrocyclization via Click chemistry is described.

184 osphors and polymer matrices via Diels-Alder click chemistry is devised as a method.

185 hogonality and versatility of the thiol-ene "click" chemistry is expected to allow the a la carte che

186 Strain-promoted "click" chemistry is used to post-synthetically modify th

187 ng which sulfur(VI) fluoride exchange-based "click chemistry" is currently the most prominent.

188 ctynes with azides, also called "copper-free click chemistry", is a bioorthogonal reaction with wides

189 as well as their myriad of applications in "click" chemistry it is interesting to note that the synt

190 We present the use of a new form of click chemistry known as SuFEx (sulfur(VI) fluoride exch

191 review will focus on recent applications of click chemistry ligations in the preparation of imaging

192 As with most of click chemistry, many essential features of sulfur(VI) f

193 also included an alkyne group to facilitate click chemistry-mediated conjugation of reporter tags fo

194 ia, enabling in vivo photo-cross-linking and click-chemistry-mediated analysis of mycolate-interactin

195 e analogue UV cross-linked and visualized by click-chemistry-mediated fluorescent labeling.

196 Here we describe the application of a new click chemistry method for fluorescent tracking of prote

197 inking of BT to a biotin-linked moiety using click chemistry methods and coating of BT with nonreacti

198 tion, controlled radical polymerization and "Click Chemistry" methods have significantly evolved over

199 tein nanocapsules incorporating copper-free "click chemistry" moieties, polyethylene glycol (PEG) uni

200 tion (also known as copper-free azide-alkyne click chemistry), nanoparticles bearing a high-density s

201 Click chemistry of alkyne-modified RNA with different re

202 Ruthenium catalyzed 1,3-cycloaddition (click chemistry) of an azido moiety installed on dihydro

203 The cyclopropenone-based phototriggered click chemistry offers exciting opportunities to label l

204 d utilizing carbonate ester and azide-alkyne click chemistries on the surface of the closo-B(12)(2-)

205 gical and medical sciences by application of click chemistry or classic esterification and amidation.

206 ent probe cyanine 5 at their focal point via click chemistry permitted the evaluation of their cellul

207 Using click chemistry, PLA-b-PEG400-N(3) and PLA-b-PEG2000 blo

208 Click chemistry plays a dual role in the design of new c

209 Combining the siloxane crosslinker with click chemistry produces exchangeable LCE (xLCE) with tu

210 Additionally, in silico modeling of click-chemistry products may prove useful in rational dr

211 Click chemistry, proposed nearly 20 years ago, promised

212 phthalocyanines was attained by means of a "click" chemistry protocol.

213 Modifying Janus particles through thiol-yne click chemistry provides a fast-reacting, scalable synth

214 Click chemistry provides extremely selective and orthogo

215 tein of interest with high sensitivity using click chemistry, proximity ligation and fluorescence mic

216 tion sequencing) library synthesis that uses click chemistry rather than enzymatic reactions for the

217 4-5-step process, involving as a key step a click chemistry reaction between ynamides and azides.

218 By taking advantage of a facile, one-pot click chemistry reaction, we report herein the preparati

219 corporated OP-Puro can be detected through a click-chemistry reaction that links it to a fluorescentl

220 ogs followed by a fluorescent azide-coupling click-chemistry reaction.

221 Two click chemistry reactions were now combined to form nucl

222 ed AutoClickChem, capable of performing many click-chemistry reactions in silico.

223 were used as reaction templates for in situ click-chemistry reactions to generate a congeneric serie

224 t supramolecular catalysts for alkyne-azide "click chemistry" reactions, yielding the desired 1,4-add

225 ive as compared to the engineered enzyme and click chemistry reagents.

226 ons via selective-pressure incorporation and click chemistry, respectively, without affecting the Ub

227 ylcyclooctyne (DBCO-Cy5.5) via bioorthogonal click chemistry, resulting in Cy5.5-labeled CTLs (Cy5.5-

228 exciting reaction to other well-established click chemistry schemes, its high reaction speed and its

229 through the use of single-generation in situ click chemistry screens against large peptide libraries.

230 Though both in silico screening and click chemistry seek to make drug discovery more feasibl

231 dendrimers synthesized from Cu(I)-catalyzed click chemistry showed a high level of copper contaminat

232 Use of the propargyl ether 44 and Click chemistry showed that niphatenone B binds covalent

233 uromutilin derivatives were synthesized by a click chemistry strategy.

234 te a carbohydrate array via efficient local "click" chemistry strategy.

235 vantages of highly chemo- and regioselective click chemistry, such as mild reaction conditions, effic

236 unched AChE as a reaction vessel for in situ click-chemistry synthesis of high-affinity TZ2PA6 and TZ

237 e in situ reaction products established that click-chemistry synthesis with surrogate receptor templa

238 uses a novel synthetic photoactivatable and click chemistry-taggable phospholipid probe, which, when

239 We employ an efficient "click chemistry" technique to synthesize aptamer-polymer

240 by a combination of e-beam lithography and "click" chemistry techniques.

241 of efficient blue-emitting fluorophores via click chemistry that could be potential luminophores in

242 We confirmed using 5-ethynyl-2'-deoxyuridine click chemistry that the Oc-Cre lineage includes very fe

243 This review is focused on the emerging click chemistry that uses mesoionic compounds as dipole

244 ent of selective bioorthogonal reactions or "click" chemistry that can proceed in live animals.

245 abolic labelling combined with bioorthogonal click chemistry (that is, reactions performed in living

246 eactions have been developed, exemplified by click chemistry, that enable the efficient formation of

247 Combining ATRP signal amplification with "Click Chemistry", the optimized DNA biosensor was capabl

248 e caged dye to the substrate of interest via click chemistry, the allyloxy appendage was functionaliz

249 Using click chemistry, the overexpressed sterol regulatory ele

250 re constructed according to the reactions of click chemistry, they can be easily synthesized for subs

251 Using copper-assisted click chemistry, they can be functionalized with a varie

252 re, we introduce several approaches based on click chemistry, through which we study the distribution

253 r cells and perform efficient, biocompatible click chemistry, thus acting as intracellular nanoscale

254 ve developed a modification of bioorthogonal click chemistry to assay the palmitoylation of cellular

255 sists uptake by cells allowing extracellular click chemistry to be performed.

256 Here, we successfully use click chemistry to construct DNA templates for sgRNA exp

257 l coupling of multifunctional monomers using click chemistry to create a branched cross-linked polyme

258 C termini using sortase transpeptidation and click chemistry to create a covalently linked IgG antibo

259 zed peptides were synthesized by solid phase click chemistry to develop novel, potent, selective MC4R

260 ic modification of PG can be extended to use click chemistry to fluorescently label the mature PG in

261 For this purpose, we developed in situ click chemistry to functionalize SPR sensor chips.

262 polymer backbone by exploiting azide-alkyne click chemistry to functionalize the hydrogels with a pe

263 e picture) were subjected to high-throughput click chemistry to generate a library of sialic acid ana

264 e cell labelling with azidomyristic acid and click chemistry to identify N-myristoylated proteins in

265 ligosaccharide engineering and bioorthogonal click chemistry to label various commensal anaerobes, in

266 Here, we use click chemistry to label viruses with integrase coupled

267 olyprotein construct and DNA via copper-free click chemistry to PEG-coated substrates and a strong bu

268 demand (EVOD) chip utilizes a catalyst-free click chemistry to rapidly and specifically isolate EVs

269 canonical amino acid tagging method, we used click chemistry to study the role of protein synthesis a

270 lkynylphosphonate can be functionalized via "Click" chemistry to generate the 1,2,3-triazolyl or subs

271 Here, we present a simple strategy using 'click' chemistry to couple biotin to a 'caged' photoclea

272 ere surface functionalized with folate using click-chemistry to improve targeted uptake by the malign

273 Using "click chemistry" to detect 5-ethynyl-2'-deoxyuridine (Ed

274 to activate cellulosic materials for CuAAC "click chemistry", to develop new fluorogenic esterase se

275 t reaction (thiol-acrylate/thiol-ene 'double-click' chemistry) to obtain highly uniform dynamically c

276 SuFEx is a new-generation click chemistry transformation that exploits the unique

277 tionally designed and developed via a facile click-chemistry type postfunctionalization, which can fo

278 active scaffolds was synthesized by means of click-chemistry under non-conventional microwave heating

279 Click chemistry was employed to cross-link peptides to D

280 Stepwise click chemistry was employed to introduce cross-links at

281 Click chemistry was then used for the highly efficient a

282 Total synthesis combined with click chemistry was used to label the Ts1 protein molecu

283 "Click chemistry" was used to conjugate alkyne-containing

284 discovered by combinatorial copper-catalyzed click chemistry, was synthesized in situ by incubating e

285 ighly DNA specific and efficient enzyme, and click chemistry, we demonstrated that as little as 0.1 f

286 Using click chemistry, we labeled newly synthesized proteins a

287 cancer associated surface proteins into the click-chemistry, we were able to selectively recover can

288 can be covalently attached to surfaces using click chemistry, where they retain their abilities to in

289 ), and rapid and efficient radiolabeling via click chemistry with (18)F-labeled trans-cyclooctene ((1

290 diabody A2, and subsequently radiolabeled by click chemistry with (18)F-TCO.

291 with either DML or sCy5 and radiolabeled via click chemistry with (18)F-TCO.

292 can be employed for toxin derivatization by click chemistry with an azide-containing reporter molecu

293 by using fluorescent SABRAC analogues or by click chemistry with an azide-substituted analogue.

294 The merging of click chemistry with discrete photochemical processes ha

295 ght into this dynamic interface, we combined Click chemistry with pulsed stable isotope labelling of

296 ith ease", by combining classic C-C pai-bond click chemistry with recent developments in connective S

297 tes by combining stable isotope labeling and click chemistry with subsequent mass spectrometry analys

298 t of the ligand to a surface by conventional click chemistry without disturbing selective interaction

299 lysis of ~ 300 derivatives synthesized using click chemistry yielded compounds with greatly enhanced

300 of new biotin 1,2,3-triazole analogues using click chemistry yielded our most potent structure (K(i)