ライフサイエンスコーパス: clonal

1 is typically assumed that these isolates are clonal.

2 erimental models that can be used to examine clonal abnormalities are limited.

3 distinct mechanisms by which mutations drive clonal advantage in each disease and their associations

4 Clonal amplification of one or a few viral sequences acc

5 ate-like biology, albeit with some scope for clonal amplification.

6 lineages and opening up an important form of clonal analysis in cancer.

7 he course of a month and is also amenable to clonal analysis of HSC heterogeneity.

8 Here we combine clonal analysis, genetic lineage tracing, tissue transpl

9 Using genetic lineage tracing, clonal analysis, multiplexed in situ hybridization, immu

10 Using explant studies, lineage tracing, and clonal analysis, we demonstrate that Ift20 is required f

11 Using clonal analysis, we show that Delta (Dl) serves as the l

12 Clonal and anatomical analyses suggested that a single F

13 by meiotic gynogenesis and were verified as clonal and identical to their mother with microsatellite

14 We also describe assays for EC clonal and network formation, as well as transcriptomic

15 ealistic tumor genomes that harbor all known clonal and subclonal mutation types and processes.

16 was inferior in patients with relapses with clonal and subclonal NT5C2 mutations compared with those

17 es allowed for the detection and tracking of clonal and subclonal tumor cell populations.

18 Clonal animals do not sequester a germ line during embry

19 or was designed based on interaction of poly clonal antibody conjugated with graphene quantum dot wit

20 Alzheimer's disease and provide evidence of clonal, antigen-experienced T cells patrolling the intra

21 within individual aphids and among different clonal aphid lineages, and is affected by environmental

22 Using a clonal approach, we previously detected 200-400 mosaic S

23 Clonal approaches such as this enhance the resolution of

24 ow substantial SCNA and methylation ITH, and clonal architecture analyses present congruent evolution

25 a single-cell DNA sequencing, we report the clonal architecture and mutational histories of 123 acut

26 istribution of recurrently mutated genes and clonal architecture differed among MDS/MPN subtypes.

27 ted way to interactively examine the spatial clonal architecture of a tumor, facilitating clinical an

28 ionary bottleneck, we observe a more complex clonal architecture over time, and appearance of unrelat

29 mors comprises thousands of subclones, has a clonal architecture similar to primary tumors, and is de

30 pertoires started as diverse but became more clonal at the late time point.

31 c variation in dehydrin gene expression in a clonal bank comprised of all three ecotypes.

32 lineage relationships after a single step of clonal barcoding.

33 monstrate the emergence and persistence of a clonal C. auris population and highlights the importance

34 c heterogeneity of circadian oscillations in clonal cell populations to investigate the underlying me

35 lar compartments and cannot be propagated in clonal cells for longitudinal studies or used for in viv

36 We validate our technique in clonal cells with previously defined integration sites a

37 own as i-cells, to the germ cell fate in the clonal cnidarian Hydractinia symbiolongicarpus Tfap2 mut

38 ae (KPC-KP) sequence type (ST) 16 clone in a clonal complex (CC) 258-endemic setting.

39 s), termed "high-risk clones." We noted that clonal complex (CC) 446 (which includes STs 298 and 446)

40 e changed over time, with a 1.5x increase in clonal complex (CC)8 strains and a concomitant decrease

41 p W (IMD-W) cases caused by sequence type-11 clonal complex (cc11) was observed from October 2015 in

42 a hypervirulent strain belonging to the ST11 clonal complex.

43 5 MRSA isolates studied were grouped into 23 clonal complexes (CCs) and assigned to 103 strains.

44 thogenesis of myeloid transformation and how clonal complexity evolves with disease progression.

45 cur in the same clone(s), accurately measure clonal complexity, or definitively elucidate the order o

46 Here, we studied the clonal composition of B cell infiltrates using 4 graft s

47 ung cancer model, showing that it shifts the clonal composition of the tumour in our favour, leading

48 coverage ~0.03x, SBMClone recovers the major clonal composition when incorporating a small amount of

49 es, producing recurrent tumors with distinct clonal composition, genetic alterations, and drug sensit

50 We find that the initial B-cell clonal composition, T-follicular helper cell signaling,

51 This suggests the value of monitoring clonal constitution and tumor microenvironment over time

52 e show that this process plays a role during clonal contraction, establishment of immune memory, and

53 uence comparison between patients identifies clonal convergence.

54 MBC-like WM harbored significantly more clonal CXCR4 mutations (P = .015), deletion 13q (P = .00

55 oiesis of indeterminate potential (CHIP) and clonal cytopenias of undetermined significance (CCUS).

56 ng tissues is commonly assessed by analysing clonal data from in vivo cell lineage-tracing assays.

57 , rendering the selection of the appropriate clonal deconvolution method critical.

58 nd limiting autoimmunity through both thymic clonal deletion and Treg cell generation.

59 ile B7-CD28 interaction is required for both clonal deletion and Treg induction, these two processes

60 Meanwhile, defective thymic clonal deletion due to altered B7-CD28 signaling results

61 ounterparts in Tgm2-/- mice with no signs of clonal deletion, receptor editing, or B cell anergy.

62 Notably, at clonal density, the colony-forming capacity of MSCs was

63 Subcloning these late passage cells to clonal density, to mimic lung injury in vivo, selects fo

64 While clonal depletion of the NMDA receptor subunit NR2 result

65 dependently, aggregation was far superior to clonal development, providing a 35% advantage during gro

66 Together, these data unravel clonal diversity and evolution patterns of AML, and high

67 ll analyses, including the interpretation of clonal diversity and proliferation of intra-tumor lympho

68 We find that clonal diversity decreases during tumor regression, resi

69 esses, fitted to previously published HTLV-1 clonal diversity estimates.

70 Clonal diversity is a consequence of cancer cell evoluti

71 TCRB sequencing shows that clonal diversity of CAR-T cells is highest in the IPs an

72 Clonal diversity results in clone-specific expansion wit

73 -induced antibodies exhibit a high degree of clonal diversity, recognize five conformational antigeni

74 recall responses are confined by preexisting clonal diversity.

75 stinct subclones, consistent with increasing clonal diversity.

76 imately half of the recurrent tumors exhibit clonal dominance with a small number of subclones compri

77 ients with 2-5 serial plasma samples reveals clonal dynamics and genome evolution in response to horm

78 tionary approach to non-invasively delineate clonal dynamics and identify clones with mutations assoc

79 tigated the impact of immune pressure on the clonal dynamics and immune escape signature by comparing

80 We use cellular barcoding to monitor clonal dynamics during tumor recurrence in vivo.

81 Our work provides a mechanism for how early clonal dynamics imprint the hierarchy of T cell clone si

82 onitoring responses to therapy, and tracking clonal dynamics in response to targeted therapies and ot

83 Computational modelling of clonal dynamics indicates that high genetic heterogeneit

84 To address this issue, we explored the clonal dynamics of memory inflation.

85 There was no molecular evidence of either clonal dysregulation or transgene silencing.

86 atients typically relapse, most often due to clonal emergence of the resistance-associated KIT V654A

87 e whole-genome doubling, that was found as a clonal event in 49% of tumours.

88 poptosis, with a higher probability of tumor clonal evolution and resistance.

89 r results support a role of the epigenome in clonal evolution and uncover new candidate pathways asso

90 ylogenetic analysis demonstrated the lack of clonal evolution for African strains which conclusively

91 erstanding of the molecular events governing clonal evolution in MPNs, the cell-intrinsic and -extrin

92 rvival genes in cancer cell integrity during clonal evolution in non-small cell lung cancer (NSCLC).

93 Drug resistance mediated by clonal evolution is arguably the biggest problem in canc

94 y cause a malignant phenotype and continuous clonal evolution is often linked to tumor progression.

95 Revealing the clonal evolution mechanisms in the metastatic transition

96 Here we investigate ITH and clonal evolution of papillary renal cell carcinoma (pRCC

97 by providing a more detailed picture on the clonal evolution of the tumor.

98 ls; (2) colony-forming units (CFUs) revealed clonal evolution or multiple independent clones in indiv

99 ed using whole-exome sequencing to infer the clonal evolution patterns.

100 tations at progressive disease (PD), and the clonal evolution remain underinvestigated.

101 s aiming to understand the mechanisms of HSC clonal evolution will benefit from this new approach to

102 ms and risk stratification tools, studies of clonal evolution, and prospective trials are needed to i

103 cterized by linear and branching patterns of clonal evolution, with the latter including convergent e

104 reased BCR signaling, CLL proliferation, and clonal evolution.

105 did not, suggesting that treatment promotes clonal evolution.

106 h intra-individual chromatin variability and clonal evolution.

107 and characterizing mutagenesis and the early clonal evolutionary hallmarks of carcinogenesis.

108 ugh persistent antigen exposure favors their clonal expansion and accumulation of mutations, which fu

109 ions of mutations that synergized to promote clonal expansion and dominance.

110 ulation, T cells exit quiescence to initiate clonal expansion and effector differentiation.

111 etic mutations through a stepwise process of clonal expansion and evolution.

112 possessing functional basal cells capable of clonal expansion and multilineage differentiation.

113 lly, we show increasing B cell accumulation, clonal expansion and mutational frequency from the cecum

114 The mechanisms involved in clonal expansion and persistence need to be defined to e

115 We analyzed IgG repertoire diversity, clonal expansion and somatic hypermutation in cells from

116 ingle-cell RNA sequencing, which demonstrate clonal expansion and unique functional states of B cells

117 We also show the consistency of clonal expansion dynamics between bulk alpha and beta re

118 Overall, our studies demonstrated B cell clonal expansion in human cardiac allografts with CAV.

119 Here, we summarize the evidence for clonal expansion in innate lymphocytes, which has primar

120 cquisition of somatic mutations that lead to clonal expansion in regenerating haematopoietic stem cel

121 Results showed robust B cell clonal expansion in the graft but not in the blood for a

122 Clonal expansion not only serves to amplify the number o

123 resistance from single cells, mimicking the clonal expansion of a resistant lineage following mutati

124 tectable by bulk sequencing only in cases of clonal expansion of a single cancer cell bearing the mut

125 expression in two dogs may have been due to clonal expansion of cells harboring integrated vectors.

126 nd are probably, as in adults, maintained by clonal expansion of cells infected before ART initiation

127 of indeterminate potential (CHIP) refers to clonal expansion of hematopoietic stem cells attributabl

128 dom lineage tracing to localize and quantify clonal expansion of hepatocytes in normal and injured li

129 at ART reduces HIV-infected T cells and that clonal expansion of HIV-infected cells maintains viral p

130 In vivo clonal expansion of HIV-infected T cells is an important

131 ty of the HIV-1 reservoir by stimulating the clonal expansion of latently infected CD4+ T cells.

132 ndrome coronavirus 2 (SARS-CoV-2) led to the clonal expansion of SARS-CoV-2-specific CD8(+) and CD4(+

133 We detect different clonal expansion of the adaptive immune system in distan

134 However, single-cell clonal expansion produces heterogeneous methylomes, thus

135 enables them to overtake the tissue through clonal expansion that causes, but also relies on, the ki

136 ichment of CD133+ cells by controlling their clonal expansion through the Wnt-regulator FZD8.

137 ounder cells subsequently undergoing massive clonal expansion to form occlusive neointimal lesions.

138 quisition of oncogenic mutations and grow by clonal expansion(1,2).

139 tory cytokines are required to drive NK cell clonal expansion, additional stimulatory signals control

140 ergone minimal somatic mutation with limited clonal expansion, and three bound the receptor-binding d

141 rom the fundamental immunological process of clonal expansion, highlighting the parallels between inn

142 The roles distinct B cell subsets play in clonal expansion, isotype switching, and memory B cell d

143 Also, tonic signaling and clonal expansion, two important functions mediated by th

144 an innate-like low cytotoxic state, with low clonal expansion, unlike the classical exhausted state o

145 ve horizontal spread of mcr-1 rather than by clonal expansion.

146 ls without prior GC experience or detectable clonal expansion.

147 es, and across lymphocyte subsets; to detect clonal expansion; and to detect the recruitment of new c

148 We find that clonal expansions during a perinatal time window leave a

149 ssibility of identifying and tracking B cell clonal expansions during adaptive immune responses.

150 habeta analyses of MAIT cells revealed large clonal expansions in older adults and tissues that rival

151 In contrast to the normal colon, where clonal expansions outside the confines of the crypt are

152 s with a selective advantage, leading to the clonal expansions responsible for dominant genetic disea

153 delta1 and distinguished by highly localized clonal expansions, consistent with the nonrecirculating

154 are, we observed widespread millimeter-scale clonal expansions.

155 These nAbs belonged to 21 clonal families and employed a variety of VH genes.

156 (gamma9delta2TCRs), was not associated with clonal frequency.

157 Moreover, these highly uropathogenic clonal groups demonstrate an especially prolonged gut pe

158 use many epiphytic vascular plants undertake clonal growth and because vascular epiphytes colonize ca

159 sease-causing somatic mutations can initiate clonal growth prior to the appearance of any disease sym

160 cancer and of risk-matched controls reflect clonal haematopoiesis and are non-recurrent.

161 is Viewpoint assesses recent developments in clonal haematopoiesis and the related implications for a

162 CHIP through mechanisms that are specific to clonal haematopoiesis as well as shared mechanisms that

163 Compared with tumour-derived mutations, clonal haematopoiesis mutations occur on longer cfDNA fr

164 ysplastic syndrome precursor states, such as clonal haematopoiesis of indeterminate potential (CHIP)

165 y heart disease(5)-this phenomenon is termed clonal haematopoiesis of indeterminate potential (CHIP)(

166 ated with greatly increased vulnerability to clonal haematopoiesis with specific acquired CN-LOH muta

167 When faced with a diagnosis of clonal haematopoiesis, some patients and providers might

168 be justified by our present understanding of clonal haematopoiesis.

169 e we study this phenomenon in the context of clonal hematopoiesis (CH) and the development of therapy

170 Recent studies identified nearly ubiquitous clonal hematopoiesis (CH) in AA patients.

171 The discovery of clonal hematopoiesis (CH) in older individuals has chang

172 Clonal hematopoiesis (CH) is common in older persons and

173 t that many treated patients have persistent clonal hematopoiesis (CH) that may not reflect residual

174 hly variable, ranging from clinically silent clonal hematopoiesis (CH) to leukemic progression.

175 DNMT3A) is the most commonly mutated gene in clonal hematopoiesis (CH).

176 re subject to frequent missense mutations in clonal hematopoiesis and diverse neoplastic diseases.

177 Somatic mutations leading to clonal hematopoiesis have been described in IBMFSs, but

178 understanding of the processes that promote clonal hematopoiesis in IBMFSs may inform clinical surve

179 Clonal hematopoiesis is exceptionally common with human

180 vascular event in an individual patient with clonal hematopoiesis may be low, the possibility of futu

181 People with clonal hematopoiesis may come to clinical attention in a

182 mutations in the peripheral blood is termed clonal hematopoiesis of indeterminate potential (CHIP) a

183 Clonal hematopoiesis of indeterminate potential (CHIP) r

184 atients without MDS/AML also had evidence of clonal hematopoiesis of indeterminate potential-related

185 e is often preceded by a premalignant state (clonal hematopoiesis or myelodysplastic syndrome).

186 hieve AML cure and the impact of preleukemic clonal hematopoiesis persistence in predisposing to seco

187 Here, we will review recent studies linking clonal hematopoiesis to altered immune function, inflamm

188 Emerging data also associate clonal hematopoiesis with other nonhematologic diseases.

189 erved in hematological malignancies(1-3) and clonal hematopoiesis(4,5).

190 hape the genetic diversity of healthy blood (clonal hematopoiesis).

191 odysplastic syndromes and are also common in clonal hematopoiesis, acute myeloid leukemia, chronic ly

192 es clinical considerations for patients with clonal hematopoiesis, including important points for hem

193 rdiovascular disease and are associated with clonal hematopoiesis, inflammation, and adverse vascular

194 ction, not drift, is the major force shaping clonal hematopoiesis, provide bounds on the number of he

195 es and T cells of patients with HF harboring clonal hematopoiesis-driver mutations in DNMT3A exhibit

196 2 gene in hematopoietic cell development and clonal hematopoiesis.

197 d for NGS may contain germline, somatic, and clonal hematopoietic DNA alterations, and distinguishing

198 low variant allele fraction (VAF < 15%) and clonal heterogeneity contribute up to 68% of private WGS

199 Early cultures maintain the clonal heterogeneity seen in PDAC with similar phenotype

200 TAFRO might share pathogenetic features with clonal inflammatory disorders bearing MEK and RUNX1 muta

201 loid cells contained large numbers of likely clonal insertions.

202 Both clonal integration and environmental buffering from nonv

203 Overall, clonal integration had greater benefits than inter-speci

204 epiphytes played a more important role when clonal integration of vascular epiphytes was eliminated.

205 Parasitism can cause clonal integration to negatively affect fitness in clona

206 However, clonal kinetics and transcriptional programs that regula

207 rve clones that display distinct patterns of clonal kinetics, making variable contributions to the CA

208 n site does not appear to be a key driver of clonal kinetics, scRNA-seq demonstrates that clones that

209 ticarbohydrate-binding patterns, observed at clonal level as well, could explain these apparently opp

210 ex variety of regenerative behaviours at the clonal level, but the mechanisms underlying this diversi

211 It can provide clonal-level insights into cellular proliferation, devel

212 ly by stable association with one successful clonal lineage (ST258/512) yet have been mobilized among

213 By combining in vivo clonal lineage tracing, proliferation kinetics, single-c

214 It was found that boosting increased clonal lineage-specific ADCC breadth and potency.

215 an match or transcend copy number-determined clonal lineages and opening up an important form of clon

216 We further reveal large clonal lineages formed by tandem fusions in antigen-stim

217 ly thought and that about a quarter of large clonal lineages with unusually long CDR3s are generated

218 mitotic gynogenes, and from these the first clonal lines in Atlantic salmon.

219 first maturing females, five all homozygous clonal lines were produced by meiotic gynogenesis and we

220 al for production of further Atlantic salmon clonal lines, potentially with distinct characteristics.

221 Clonal loss of functional TP53 is significantly associat

222 ulent gene-containing SM cluster, ACE1, in a clonal M. oryzae population (Clade 2).

223 Although clonal mast cell disease is the culprit in some individu

224 6V, and bone marrow evaluation to rule out a clonal mast cell disorder.

225 Patients with clonal mast cell disorders (cMCD), systemic mastocytosis

226 se level (MCAS-T) may not necessarily have a clonal MC disorder.

227 The results of the work-up for clonal MC disorders such as systemic mastocytosis and mo

228 ples, at known proportions, created from two clonal metastases from the same patient.

229 g data, we show that Epiclomal discovers sub-clonal methylation patterns in aneuploid tumour genomes,

230 uM Ag(+), suggesting that a subpopulation of clonal MSCs was sensitive to Ag(+) exposure.

231 by genomic heterogeneity and presence of sub-clonal mutations.

232 clonal mutations and only one-third harbored clonal mutations.

233 emia is characterized by the accumulation of clonal myeloid blast cells unable to differentiate into

234 immune escape, tumor neoantigens are either clonal or at low frequency; hypermutated tumors can only

235 and the adjacent tumor, suggesting a shared clonal origin, as well as instances in which both NAT ti

236 NA at recurrence further helped identify the clonal origin.

237 ation, confirming cellular malignancy with a clonal origin.

238 into hematologic malignancies; however, the clonal origins of such malignancies remain unknown.

239 integration to negatively affect fitness in clonal plants because parasites can import resources fro

240 pairs of connected ramets of two congeneric clonal plants, Sphagneticola trilobata and Sphagneticola

241 affect the response of individual cells in a clonal population to cisplatin, a DNA-damaging chemother

242 The clonal populations are engineered with secreted Gaussia

243 fitness landscapes, the instability rates of clonal populations form their mutability landscapes.

244 Here, we describe the generation of clonal populations from a patient-derived ovarian clear

245 We distinguish these possibilities by using clonal populations of yeast cells to quantify the inhere

246 oniae have shown that hsdS inversions within clonal populations produce subpopulations with profound

247 We find that tumors derived from the same clonal populations showed heterogeneous ICB response and

248 es that can be used in vivo in mice to track clonal populations.

249 r in rare cells within otherwise homogeneous clonal populations.

250 As a result, MB-like clonal precursors become trapped in an oscillatory state

251 osed of candidate phylogenies and associated clonal prevalence.

252 Even though the risk of clonal progression or a cardiovascular event in an indiv

253 pus-prone mice and patients with SLE undergo clonal proliferation and expansion in a self-antigen dep

254 he study of cellular population dynamics and clonal properties in vivo.

255 rising the vast majority of the tumor; these clonal recurrences are frequently dependent upon Met gen

256 Clonal relatedness between the actor (brain fluke) and r

257 To date, however, the precise clonal relationship between GCTs and the diverse additio

258 ences of MLL-ENL expression based on a clear clonal relationship between parental and leukaemic cells

259 he JCI, Taylor, Donoghue, et al. unravel the clonal relationship between primary mediastinal nonsemin

260 gnancy and prior data intriguingly suggest a clonal relationship exists between hematologic malignanc

261 NA (mtDNA) mutations enable the inference of clonal relationships among cells.

262 ll phenotypes in health and UC, define their clonal relationships and characterize terminally differe

263 Clonal relationships are learned from AIRR-seq data by a

264 racing methods now facilitate the mapping of clonal relationships onto these landscapes and enable de

265 Here, we examined the anatomic distribution, clonal relationships, and functional properties of T-bet

266 tes p53, which substantially shrinks the HSC clonal repertoire in hematochimeric mice, although engra

267 acterial antigens on the emergent B-1 B cell clonal repertoire.

268 While apomixis, that is, clonal reproduction, is intuitively antithetical to dive

269 These trade-offs reveal that clonal research and development, strategic corporate and

270 The clonal resistant subline K/VP.5 contains reduced TOP2alp

271 ep sequencing data can be used to assess the clonal richness and diversity of lymphocyte populations;

272 which is only slowly reshaped by fluctuating clonal selection during adult life.

273 n-specific patterns of genomic mutations and clonal selection in haematopoietic cells on the basis of

274 While clonal selection in response to particular antigens has

275 The extent of somatic mutation and clonal selection in the human bladder remains unknown.

276 owed to be an appropriate tool to assist the clonal selection process of 'Tempranillo'.

277 pecific to that antigen are enriched through clonal selection, expansion, and somatic hypermutation.

278 erving cell-cell interactions and minimizing clonal selection, GBOs maintain the cellular heterogenei

279 rations in the epigenome might contribute to clonal selection, yet the extent to which the chromatin

280 ir prognostic significance and mechanisms of clonal selection.

281 e to therapy has led to a Darwinian model of clonal selection.

282 Clonal sequence type (ST)-306 and ST615 are representati

283 Twenty-four (83%) of the 29 LC clonal sequences found were derived from the IGLV1-40 an

284 The clonal shift was reflected in AMR patterns, with a decre

285 owing for potent and targeted suppression of clonal SMC expansion in the plaque in vivo.

286 revealed that this prevalence was not due to clonal spread in clinic but rather to an intermingling o

287 l fate choice predict sufficiently different clonal statistics.

288 edict, under asymptotic conditions, the same clonal statistics.

289 tiation and growth, including tumor hypoxia, clonal stem cell selection, and immune cell response, al

290 arranged, long-lived but dormant epithelial clonal stem cells mutants.

291 sal") functional dependence of the long-term clonal survival probability on distance from the niche,

292 Repertoire analysis revealed broad clonal T cell expansion and curtailed interferon respons

293 etic stem and progenitor cells (HSPCs) using clonal tracking in patients treated with gene therapy fo

294 Here, we apply a barcoding strategy to clonal tracking of edited cells (BAR-Seq) and show that

295 We mapped clonal trajectories for each sample and uncovered combin

296 logy to reconstruct alpha-beta pairings from clonal trajectories.

297 alterations in chromosome structure underlie clonal transcriptomic, epigenomic, and histopathologic s

298 ogenic autoantibodies were found to comprise clonal trees accumulating mutations.

299 perties and haemolytic activities of these 2 clonal types using in vivo mouse models and in vitro ass

300 were frequently subclonal yet appeared to be clonal within single samples.