ライフサイエンスコーパス: clone

1 ing heterogeneity and evolution of resistant clones).

2 neage might constitute an emerging high-risk clone.

3 of Kpi may explain the success of the ST-15 clone.

4 g the number of genetic variants assayed per clone.

5 system is unable to eradicate the malignant clone.

6 n worldwide, caused by the emerging ST-13578 clone.

7 ion (SD) of individual root measurements per clone.

8 f myeloma cell evolution in a drug-resistant clone.

9 tructure, it is also a characteristic of the clone.

10 ignment to a sub-study based on the dominant clone.

11 eting efficiency up to 76.5% of total mutant clones.

12 l technology with which to track single-cell clones.

13 ed by increased prevalence of high-frequency clones.

14 and may be ineffective in killing particular clones.

15 netic trees of the PMNs and the ensuing HSTM clones.

16 d genes between cells from different somatic clones.

17 d stable cell lines and isolated single-cell clones.

18 evidence of substantial cell death in Cit(+) clones.

19 Sanger sequences of over 90 Y chromosome BAC clones.

20 ion of distinct populations of cells, called clones.

21 eity through the selection of immune-evasive clones.

22 improved growth characteristics in specific clones.

23 , produced by USA300 and other epidemic MRSA clones.

24 cture over time, and appearance of unrelated clones.

25 ransfected with 2 spectrally different OLIG2 clones.

26 epigenetic variation in plants propagated as clones.

27 one, a single broadly reactive clone or both clones.

28 ivity and specificity for identifying B cell clones.

29 tability were maintained in all heterozygous clones.

30 ogenic STAT5 and ERK activation to competing clones.

31 ichment of pre-existing ICB-resistant cancer clones.

32 he relevant gene was identified by map-based cloning.

33 th lymphocytic choriomeningitis virus (LCMV) clone 13 (CL13) are highly susceptible to mousepox, a di

34 .IMPORTANCE Infection of adult mice with the clone 13 (CL13) strain of lymphocytic choriomeningitis v

35 th lymphocytic choriomeningitis virus (LCMV) clone 13 (CL13), result in immune dysfunction that predi

36 We cloned 17 AAE genes, expressed them heterologously in Es

37 We confirmed these associations by cloning 22 different alleles and expressing them in a co

38 ed the repertoire of known HBoV1 variants by cloning 29 distinct HBoV1 capsid sequences from primary

39 Cloned 30 years ago, it was characterized as a flavoenzy

40 Moreover, clone 46.1-scFv was found to associate with postvascular

41 travenous administration with one particular clone, 46.1-scFv, exhibiting a 26-fold increase in brain

42 2 Fc-glycosylation features of 23 mAb-Ds (12 clones, 5 produced from multiple cell lines) and one blo

43 n the overall disease burden of JAK2-mutated clones(6,7), prompting us to investigate the mechanism u

44 Cinsault, Gewurztraminer (clone 643), Moscatel de Alejandria, Pais, Pinot Noir (Fr

45 atel de Alejandria, Pais, Pinot Noir (French clone 777), Pinot Noir (local clone 'Valdivieso').

46 mparable with the entire population of 1,485 clones (9-15% and 85-91%, respectively).

47 so restored by the exogenous expression of a cloned A* gene.IMPORTANCE Double-stranded DNA viruses ty

48 ells that come from a single strain-specific clone, a single broadly reactive clone or both clones.

49 of atabecestat metabolite-responsive T-cell clones activated via a pharmacological interactions path

50 delivery in mice of an infectious ZIKV cDNA clone allows the rescue of recombinant (r)ZIKV in vivo.

51 the immunological features of the malignant clone and alterations in the immune microenvironment in

52 analyses confirmed the spread of the NRCS-A clone and enabled us to date its emergence in the late 1

53 Treatment targets the underlying clone and should be risk adapted to rapidly administer t

54 on is explained by the home environment of a clone and the most common parallel genetic changes, wher

55 We cloned and analysed selected upstream regulatory regions

56 Here, we cloned and characterized Lavandula x intermedia cDNAs en

57 it variation in sorghum have been previously cloned and characterized.

58 ive GH10 endo-beta-1,4-xylanase, Xyl10E, was cloned and expressed in Escherichia coli.

59 23 alleles in the entire population of 1,485 clones and captured the genetic diversity of the entire

60 created a large library of isogenic reporter clones and identified reporter integration sites in a ma

61 ational signatures were heterogeneous across clones and individuals, which suggests differential expo

62 tion to maximize representation of Louisiana clones and minimize import of clones from Miami.

63 cell receptor repertoire with immunodominant clones and serum autoantibodies reactive to oncogenic si

64 ed information on the product quality of the clones and the effect of growth conditions in a fast and

65 igin and harvest year of 'Tempranillo' grape clones and with Partial Least Squares (PLS) regressions

66 Prospective studies targeting plasma cell clones and/or fibrotic pathways are warranted for long-t

67 porter (ClVST1) identified through map-based cloning and association study, whose expression in fruit

68 The in silico cloning and codon optimization supported the proficient

69 Here, we report cloning and expression of a novel P2RX7 splice variant,

70 We report here the positional cloning and functional characterization of Pm41, a powde

71 Here, we report the map-based cloning and functional characterization of qFIRM SKIN 1

72 By cloning and isolating luminescent proteins from biolumin

73 emory T cell libraries, combined with T cell cloning and TCR sequencing, to dissect the human naive a

74 WTK2 was identified by map-based cloning and validated by transformation of a c.10-kb gen

75 d to alanine using a type A full-genome cDNA clone, and the virus progeny was analyzed for defects in

76 tudinal non-destructive monitoring of cancer clones, and mathematical modelling of tumour evolution.

77 cells, each consisting of ~2500 independent clones, and placed the pools under selection in media wi

78 are acute CRISPR-based knockout, single cell clones, and small-molecule inhibition.

79 One SV was found in two clones, and we timed its origin to ~14 wk postconception

80 RCK's reconstructions of clone- and haplotype-specific karyotypes will aid furthe

81 Members of a B cell clone are descended from a common ancestor and share the

82 responses and whether tumor-resident T cell clones are amplified following treatment.

83 g states of preparedness from which adaptive clones are better selected.

84 estimate that between 100 and 200 new HTLV-1 clones are created and killed every day.

85 Metastatic clones are formed by dispersal of cancer cells to second

86 ytogenetic and molecular features, malignant clones are frequently insensitive to traditional AML che

87 latency model using autologous CD8(+) T cell clones as biosensors of antigen presentation, neither HD

88 at eventually exit and produce multicellular clones as they move along migratory streams toward targe

89 roenvironment in maintenance of the leukemic clone, as well as in treatment resistance.

90 s from 248 samples of 62 different cell line clones at four time points in 2 h using RapidFire-time-o

91 mice contain highly dominant 'winner' B cell clones at steady state, despite rapid turnover of germin

92 We have now produced the first documented cloned Atlantic salmon lines.

93 es were identified by quantitating the phage clones before and after affinity selection and aligning

94 stochemistry (IHC) for CTNNB1 (beta-Catenin; clone beta-Catenin-1) was performed on the constructed T

95 Using the HB5 anti-CD21 antibody clone but not the Bly-4 anti-CD21 antibody clone, we det

96 ected, unparasitized ramet by 60% and of the clone by 40%, and increased biomass of the parasite by 5

97 Pigs were cloned by transfer of chromatin from swine primary fetal

98 The CF clones Strain B and Clone C were present.

99 mutant cells with PARP inhibitors, resistant clones can arise via several mechanisms, including loss

100 at even in endemic settings, highly virulent clones can rapidly emerge demanding constant monitoring.

101 The ST16 clone carried up to 14 resistance genes, including blaKP

102 We find that high-expression clones carry insertions that are not correlated with H-N

103 ings reveal that IZP-resistant S. cerevisiae clones carry mutations in genes involved in Endoplasmic

104 These data show that HTLV-1-infected T-cell clones carrying key oncogenic driver mutations can be de

105 This clone caused 88% of Sp2 during 2015-2019.

106 The transplantation of the EC clones caused arterio-occlusive PH in rats exposed to ch

107 tions are mutually exclusive in leukemic sub-clones, causing dichotomy in therapeutic target choices.

108 Presence of distinct clones, cells that differ in their genotype and/or pheno

109 These were primarily public clones, characterized by simple rearrangements of Vgamma

110 998 cholera epidemic, the invariant epidemic clone co-existed alongside highly diverse members of the

111 nflata using bacterial artificial chromosome clones collectively containing all 17 SLF genes, SLFLike

112 ent but highly variable frequencies of aphid clones containing protective symbionts; these patterns w

113 154 expression was TCR-mediated since single clones could be specifically restimulated.

114 the immunodominance of high-affinity T cell clones declined during the chronic infection phase, like

115 DACi-treated CD4(+) T cell condition induced clone degranulation.

116 of 310 induced pluripotent stem cell (iPSC) clones derived from 100 individuals allowed us to identi

117 ared with a clinical isolate, the infectious-clone-derived SARS-CoV-2 (icSARS-CoV-2) exhibited simila

118 sociation study (GWAS) using a panel of 5130 clones developed at the International Institute of Tropi

119 nopathology, whereas the multifunctional Tc1 clone did not enhance clearance or significantly alter i

120 A 309-clone diversity panel (SDP1) was developed that captured

121 Sweeps of different resistant clones dominated the population at different timepoints,

122 ire and an associated expansion of singleton clones during treatment.

123 Clone dynamics were consistent with a simple model in wh

124 possess a vast diversity of distinct NK cell clones, each with the capacity to vary along this functi

125 A clone encoding carboxymethyl cellulase activity was isol

126 al tumour 1 (DFT1) is a transmissible cancer clone endangering the Tasmanian devil.

127 Donor-origin mutant clones engrafted in recipients and expanded during the f

128 These EC clones engrafted in the pulmonary arteries.

129 We focus here on studies using cloned eRNAs to study their function as transcripts, rev

130 port a hypothesis that the ST-13578 outbreak clone evolved from ST-1504 by recombination.All tested s

131 nsformed cells, we show that near-tetraploid clones exhibit a significant increase in the number of c

132 Deleted clones exhibited an accessible chromatin conformation wi

133 Specifically, subtype C clones exhibited the lowest CD4 and tetherin downregulat

134 BCOR-WT or BCOR-mut transcripts, while other clones expressed both BCOR-WT and BCOR-mut transcripts.

135 Clones expressed CD69, CCR4, CXCR3, and CCR10.

136 CAP-O(BCOR-mut) into iPSCs, some of the iPSC clones expressed either solely the normal BCOR-WT or BCO

137 Screening large numbers of clones expressing different trispecific antibody (tsAb)

138 Clone formation capacity of neural progenitor cells isol

139 and functional profile of the Ca(V)2 channel cloned from the early-diverging animal Trichoplax adhaer

140 n of Louisiana clones and minimize import of clones from Miami.

141 ze atabecestat(metabolite)-responsive T-cell clones from patients with liver injury.

142 Clones from PMNs and HSTMs shared a common precursor, ar

143 reponderance of seeding of new metastasis by clones from primary tumors and/or existing metastases.

144 Genetic analysis of adapted clones from two independently evolving populations revea

145 of natively paired, full-length TCRalphabeta clones, from millions of primary T cells, which were the

146 the engagement of the large diversity of MBC clones generated by priming.

147 To validate these in silico results, we cloned genes encoding candidate acid phosphatases from g

148 Among carriers of large CHIP clones, genetically reduced IL-6 signaling abrogated thi

149 enetic heterogeneity in the form of distinct clone genotypes that arise over time and across differen

150 he reconstructed mutation order and inferred clone groupings varied extensively among methods.

151 The emergent clones had acquired resistance through engagement of alt

152 When ancestral clones harbor EGFR mutations, truncal mutation abundance

153 tical intact sequences, suggestive of a cell clone harboring a replication-competent provirus.

154 By isolating microrafts that contain genetic clones harboring individual guide RNAs (gRNA), we identi

155 lly infected mice, we produced over 100 CVB3 clones harboring nine unique nucleotide "barcodes." Usin

156 vent risk among participants with large CHIP clones (hazard ratio, 0.46 [95% CI, 0.29-0.73], P<0.001)

157 P=0.019), with greater risk from large CHIP clones (hazard ratio, 1.59 [95% CI, 1.21-2.09], P<0.001)

158 ce were infected with isogenic HIV molecular clones (HIV-WT, HIV-45G, and HIV-DeltaSLQ) that differ i

159 Mutant-clone HSPCs have increased expression of megakaryocyte-a

160 prostate cancer seems to arise from a single clone in the primary tumour but can exhibit subclonal he

161 /Cas9 technology to generate stable deletion clones in BCBL-1Cas9 and BC-1Cas9 cells.

162 Here, generating tetraploid isogenic clones in colorectal cancer and in non-transformed cells

163 led clonal evolution or multiple independent clones in individuals with multiple CH mutations; and (3

164 ls and significantly lower than CMV-specific clones in infectious villitis.

165 trations and phenotypes of individual T-cell clones in response to primary and secondary yellow fever

166 Furthermore, the ratio of peanut-specific clones in the effector versus regulatory T-cell compartm

167 n cells, causing regression of the malignant clones in vivo, and inducing molecular remission.

168 Current consensus for gene cloning in wheat is to manually perform many steps in a

169 age tracing confirmed that germ cells die as clones independent of intercellular bridges, suggesting

170 creating a population structure of multiple clones (infected cell populations with identical genomic

171 ions manifests itself in high variability of clone instability rates.

172 el fertility, resulting in a final map-based cloning interval of 12 kb.

173 Two hundred nine FSPs were selected and cloned into nonhuman Great Ape Adenoviral and Modified V

174 These isoforms were subsequently cloned into recombinant adeno-associated viral (AAV) vec

175 we used an animal model, wherein NC1-peptide cloned into the pCI-neo mammalian expression vector was

176 ansion; and to detect the recruitment of new clones into a tissue.

177 Identification of the lethal tumour cell clones is required to improve survival of patients with

178 We initially characterized a yeast clone isolated for carrying an LOH event at a specific c

179 High-avidity CD8+ T cell clones isolated from healthy donors killed CBFB-MYH11+ H

180 be inhibited by overexpression of ATM from a clone lacking miR-181c binding sites.

181 y taking advantage of large existing Gateway clone libraries.

182 204 cloned lines trapped diverse proteins, including 64 orth

183 populations differ in selective pressures on clones long before the development of clinically apparen

184 In contrast to smaller clones (<5% VAF), which did not affect overall survival,

185 did not take into account > 120 wild/exotic clones maintained at the USDA-ARS Sugarcane Research Uni

186 Targeted cloning, mapping, and sequencing of parental and novel t

187 PSTVd genomes from plants inoculated with a cloned master sequence revealed naturally occurring vari

188 Follow-up analyses revealed that clones may expand and decline, generally showing only a

189 Different clones may migrate simultaneously in the streams but occ

190 iversity of lymphocyte populations; to track clone members over time, between tissues, and across lym

191 creening expression profiles of cell culture clones, monitoring purification, and evaluating drug sub

192 we use the quantified symptom intensities of cloned MSV isolates in differentially resistant maize ge

193 HSTMs were derived from multiple distinct clones not detected within the PMNs.

194 Using an infectious clone of an enteric CoV, porcine epidemic diarrhea virus

195 tive partners of CjNC110 in a sheep abortion clone of C. jejuni These data were then utilized to focu

196 We found that a M. persicae clone of near-identical females established stable colon

197 Sequencing multiple clones of a single-copy gene isolated from single hyphal

198 Despite the limited panel of test antigens, clones of antigen-responsive CD4+ T cells containing def

199 Targeted progenitors establish long-term clones of both luminal and myoepithelial lineages in adu

200 ne system responds to pathogens by selecting clones of cells with specific receptors.

201 Up to 4 clones of each species were detected within persons.

202 nsuppressible viremia can be due to expanded clones of infected CD4+ T cells carrying replication-com

203 Clones of the hybrid progenies were highly heterogeneous

204 smodium vivax [Pv]) as well as with multiple clones of the same species.

205 pt for five patients (6%) with minute T-cell clones of uncertain significance accounting for 53-136 c

206 Restriction of the Ps56 genome, cloning of several fragments, and resection of the fragm

207 ouping, an estimate of genetically distinct "clones" of cancer cells can be determined for each tumor

208 in-specific clone, a single broadly reactive clone or both clones.

209 tory requires dense sequencing of individual clones or single cells.

210 Therapies targeting the mutant clones or the increased inflammatory mediators might be

211 ncer cells and that activity of the parental clone, or functional avidity of selected gamma9delta2 T

212 sequencing applications using collections of cloned ORFs as probes.

213 ogenies are used to infer mutation order and clone origins during tumor progression, rendering the se

214 st-treatment samples demonstrates that large clones overexpress genes implicated in cytotoxicity and

215 of errors can be corrected by sampling more clones per tumor, and by increasing the number of geneti

216 specific T cells of interest for single cell cloning, phenotyping, and transcriptomics.

217 These clone phylogenies are used to infer mutation order and c

218 Many computational methods produce clone phylogenies from population bulk sequencing data c

219 Overall, single clone Pinot noir grapes grown in different regions but m

220 matochimeric mice, although engrafted edited clones preserve multilineage and self-renewing capacity.

221 HIP-reactive T-cell clones produced Th1-associated cytokines and proliferate

222 This clone provides a unique opportunity to study the physiol

223 developed previously with smaller number of clones representing WCSRG did not take into account > 12

224 To positionally clone Rf4, a large F3 population derived from a cross be

225 istinguish which mutations occur in the same clone(s), accurately measure clonal complexity, or defin

226 showed limited ability to identify ancestral clone sequences present in tumor samples correctly.

227 81.8% of recurrences came from original HCC clones sharing the same plasma vh-DNA, whereas 18.2% wer

228 Unexpectedly, some evolved populations and clones show apparent declines in fitness.

229 Monoclonal antibodies derived from these clones show increased binding, compared with their unmut

230 and infectious cases; however, these T cell clones show very little overlap between subjects.

231 of Saskatoon berry cultivars and cultivation clones significantly depended on berry genotype and comp

232 Four out of 5 cases showed increased clone size in recipients compared with donors.

233 nal dynamics imprint the hierarchy of T cell clone sizes with implications for pathogen defense and a

234 ep sequencing were used to track species and clone-specific transmission to mosquitoes.

235 The cloned Sr60 also can be a useful component of transgenic

236 The CF clones Strain B and Clone C were present.

237 The emergence of clinically important clones, such as sequence types 11, 15, 101, and 258, has

238 Analysis of these high-noise clones supports a scenario of switching due to transcrip

239 GoldenBraid is a rapid, modular, and robust cloning system used to assemble and combine genetic elem

240 Here we cloned, tagged and expressed 26 of the 29 SARS-CoV-2 pro

241 rovement of clinical manifestations after PC clone-targeted treatment.

242 dy was to generate clozapine-specific T cell clones (TCC) and characterize pathways of T cell activat

243 Here we apply single-cell cloning technologies to lung tissue of patients with and

244 re, we identified autoreactive CD4(+) T cell clones that can cross-react with HLA-DR-derived self-pep

245 We observe clones that display distinct patterns of clonal kinetics

246 clonal kinetics, scRNA-seq demonstrates that clones that expand after infusion mainly originate from

247 thymus B cell repertoire and identify B cell clones that resided in the thymus and circulation before

248 We cloned the full-length EB1 cDNA for its overexpression i

249 -mediated recombineering (LLHR), we directly cloned the skt gene cluster using the Streptomyces site-

250 involve reengagement of memory B cell (MBC) clones, the diversity and specificity of which determine

251 lineage output and dynamics of gene-modified clones; this is usually challenging because of sparse sa

252 ind that responding patients have more large clones (those occupying >0.5% of repertoire) post-treatm

253 ents to use in a RACE PCR-based approach and cloned three full-length OSC transcripts from cork (QsOS

254 mics, the ability to track individual T cell clones through paired sequencing of the T cell receptor

255 Each data analysis job can be shared or cloned to disseminate the knowledge gained, thus propaga

256 s the importance of eradicating NPM1-mutated clones to achieve AML cure and the impact of preleukemic

257 Mapping TCR clones to common viral epitopes (CMV, EBV, and influenza

258 y used for LLHR, expanding the Rec/ET direct cloning toolbox and providing the possibility for rapid

259 lder than 90 years, which suggests that such clones trend towards inevitability with advancing age.

260 ers of the Broccoli aptamer into a SINV cDNA clone using sites in nsP3 (genomic RNA), the 3'UTR (geno

261 protein barcoding system which can label 128 clones using seven antibodies.

262 t Noir (French clone 777), Pinot Noir (local clone 'Valdivieso').

263 We have successfully used AutoCloner to clone various genes of interest in the Apogee wheat vari

264 Sequencing of genomes of passaged and cloned viruses revealed mutations near the N terminus of

265 six genotypes (five varieties and a pair of clones), viz.

266 An emerging ST16 clone was associated with high mortality.

267 an adoptively transferred CD8gamma13 T-cell clone was remarkably proficient at preventing chlamydia

268 r/abacavir analogue-responsive CD8(+) T-cell clones was measured using IFN-gamma ELIspot.

269 y clone but not the Bly-4 anti-CD21 antibody clone, we detected expression of CD21 on both CD4(+) and

270 sses that give selective advantage to mutant clones, we analysed genotyping data from the blood-deriv

271 cal and systemic expansion of tumor-resident clones, we analyzed renal cell carcinomas from patients

272 ganoids, and CRISPR-Cas9 genome-edited JEG-3 clones, we herein show that YAP, the transcriptional coa

273 nosa sequence types (STs), termed "high-risk clones." We noted that clonal complex (CC) 446 (which in

274 hich did not affect overall survival, larger clones were associated with increased risk for death.

275 Distinct predominant TCR clones were detected in cervical and TLSB specimens in a

276 Thymus-associated B cell clones were detected in the circulation by both mRNA-bas

277 Drug-specific T-cell clones were generated from immediate hypersensitive pati

278 AX- and Clav-specific clones were generated from patients irrespective of whet

279 110 AX-specific and 96 Clav-specific T-cell clones were generated from seven patients with positive

280 resentatives of CC17-A1, CC17-A2 and CC23-A1 clones were identified in datasets from other countries

281 Many circulating thymus-associated B cell clones were inferred to have originated and initially ma

282 These clones were more numerous among the reactive patients, a

283 regulation activities, while subtype D and B clones were most functional for both activities.

284 In the present study, 12 independent clones were obtained and were invariably ALA-responsive,

285 ctive-TGFbeta was limited or when new T cell clones were recruited into the epidermis, antigen-specif

286 pecies corroborated that two of the isolated clones were specific to wheat, barley and rye proteins.

287 isolated B cells were derived from the same clone with 100% paired IGH: immunoglobulin light chain (

288 interactions of a highly uroporphyrinogenic clone with primary macrophages were examined with the in

289 the sublineages of a cell line involving 20 clones with 2367 mutations, which returned the optimal t

290 Haematopoietic clones with acquired mutations become common with advanc

291 Cell clones with drivers often originate during the first dec

292 Hematopoietic clones with leukemogenic mutations arise in healthy peop

293 antly, these phenotypes were not observed in clones with LRRK2 truncation.

294 Deep sequencing identified numerous mutant clones with multiple genes under positive selection, inc

295 ively delineate clonal dynamics and identify clones with mutations associated with resistance in pros

296 When clones with similar competitive fitness collide, mutant

297 the existence of phenotypically distinct TPC clones within a tumor, the evolution of TPCs with diseas

298 Finally, both donor and recipient T cell clones within the rejecting kidney suggested lymphoid ag

299 can evolve even in the presence of multiple clones within the same ant host.

300 Genetic diversity of 1,485 clones within WCSRG and Louisiana (commercials, wild/exo