ライフサイエンスコーパス: cloning

1 deletions, site-directed mutagenesis and sub-cloning).

2 ple rounds of cell selection and single-cell cloning.

3 is procedure can be implemented with minimal cloning.

4 linearity-leading e.g. to universal quantum cloning.

5 he relevant gene was identified by map-based cloning.

6 mologous arms is becoming popular in routine cloning.

7 y the madC gene was identified by positional cloning.

8 -2 and isolated the causal gene by map-based cloning.

9 ications such as B cell sequencing and/or Ab cloning.

10 ts, streamlining subsequent Ab analysis, and cloning.

11 xome capture and sequencing for rapid R gene cloning.

12 nature of molecular biology methods such as cloning.

13 i strains, to develop IVA (In Vivo Assembly) cloning.

14 basis for improving the efficiency of embryo cloning.

15 and USER (Uracil-Specific Excision Reagent) cloning.

16 rted chromosomes, mutagenesis, and map-based cloning.

17 As, along with oligonucleotide sequences for cloning.

18 oning sequence by exploiting Gibson Assembly cloning.

19 library synthesis and multi-step Golden Gate cloning.

20 of S. mutans UA159 via three rounds of NabLC cloning.

21 ssion vector in combination with single-cell cloning.

22 We confirmed these associations by cloning 22 different alleles and expressing them in a co

23 We have validated the workflow by cloning 24 human proteins of biopharmaceutical value, ei

24 ed the repertoire of known HBoV1 variants by cloning 29 distinct HBoV1 capsid sequences from primary

25 ting costs of NGS-based methods, mapping and cloning a mutation of interest has become straightforwar

26 By precisely resolving or cloning a number of these loci, we demonstrate that a br

27 admium biosensing construct was assembled by cloning a single-output cadmium biosensor element, cadRg

28 Direct cloning allowed for rapid heterologous expression in Str

29 automated primer design and recombinational cloning, allowing one to rapidly go from a genomic locus

30 Positional cloning, analysis of independent alleles, and transgenic

31 9 system, are systematized for fast, modular cloning and accommodate diverse regulatory sequences to

32 Our approach requires no molecular cloning and allows a large number of cell lines to be pr

33 ith their respective motifs, using automated cloning and analysing the methyltransferases in vectors

34 (5 h), cells were processed for single-cell cloning and analyzed for chromosomally integrated HHV-6A

35 porter (ClVST1) identified through map-based cloning and association study, whose expression in fruit

36 Here, we report the cloning and brain distribution of UCN3 mRNA in a saurops

37 risk loci have been identified by positional cloning and by genome-wide association studies.

38 lete IGS sequences, obtained by conventional cloning and by Pacific Biosciences sequencing.

39 Here we report the cloning and characterization of a quantitative trait loc

40 Here, we report the cloning and characterization of barren stalk2 (ba2), a m

41 Here we present the identification, cloning and characterization of the gymnosperm Norway sp

42 ivities of single murine IgA plasma cells by cloning and characterizing large numbers of monoclonal a

43 The in silico cloning and codon optimization supported the proficient

44 Map-based cloning and complementation tests revealed that fsh is d

45 Antibody cloning and cryo-electron microscopy structures of antib

46 re-emerging as a powerful tool for molecular cloning and DNA assembly.

47 Cloning and electrophysiological characterization of duc

48 Cross-reactivity to VZV is reconstituted by cloning and expressing TCRA/TCRB receptors from T cells

49 BAC cloning and expression analyses were employed to charact

50 Here, we report cloning and expression of a novel P2RX7 splice variant,

51 In this study, we report the cloning and expression of recombinant mouse FH and three

52 Thus, further ongoing studies for molecular cloning and expression of these genes with strong promot

53 eans including the autoantibodies' molecular cloning and expression.

54 We report here the positional cloning and functional characterization of Pm41, a powde

55 Here, we report the map-based cloning and functional characterization of qFIRM SKIN 1

56 By integrating knowledge from genomics, gene cloning and functional characterization, and environment

57 By map-based cloning and functional genomic approaches, we previously

58 netic component, and so far, only positional cloning and genomewide association studies have been use

59 Here, we report the cloning and heterologous expression in camelina (Camelin

60 n nonhuman primates, as methods for P. vivax cloning and in vitro cultivation remain unavailable.

61 Shuttle vectors for facile gap repair cloning and integration into a neutral locus in Candida

62 By cloning and isolating luminescent proteins from biolumin

63 logy and reverse genetics, which enabled the cloning and manipulation of viral genomes to express gen

64 markers developed in this study will aid in cloning and marker assisted gene pyramiding of LrAp.

65 computational effort between regions through cloning and merging steps, as in the weighted ensemble m

66 d single modules compatible with Golden Gate cloning and MoClo syntax.

67 Finally, we used machine learning, cloning and peptide screens to demonstrate the specifici

68 ase chain reaction-based immunoglobulin gene cloning and recombinant expression approach was used to

69 binding modality was generated by molecular cloning and recombinant protein expression.

70 We isolated and identified TSV by map-based cloning and rescue experiments, combined with genetic, c

71 The presence of MYMIV was confirmed by cloning and Sanger sequencing from four of the six plant

72 Previous analyses of these methods relied on cloning and Sanger sequencing to evaluate their efficien

73 iously from the same samples by standard PCR cloning and Sanger sequencing, and showed that both meth

74 ment length polymorphism fingerprinting, and cloning and sequencing of full-length 18S rRNA genes.

75 Through cloning and sequencing of individual transcripts from fe

76 Cloning and sequencing of nearly full-length fragments o

77 ic screening using an effective strategy for cloning and sequencing paired single guide RNA (sgRNA) l

78 Cloning and sequencing revealed that this cardiac isofor

79 It progresses from genetics to cloning and sequencing to biochemistry to structural bio

80 ajor responses on ibrutinib using Sanger, TA cloning and sequencing, and highly sensitive and allele-

81 This wasp is unique in terms of its larval cloning and soldier larvae.

82 emory T cell libraries, combined with T cell cloning and TCR sequencing, to dissect the human naive a

83 WTK2 was identified by map-based cloning and validated by transformation of a c.10-kb gen

84 Genome resequencing, gene cloning, and activity assays revealed that the presence/

85 s, long-range polymerase chain reaction with cloning, and complementary DNA sequencing were used to c

86 provide protocols for computational design, cloning, and experimental testing of the engineered prot

87 With RACE, molecular cloning, and long read sequencing, we found a number of

88 sible for functional gene mapping, map-based cloning, and marker-assisted breeding.

89 genic pairs of cells that avoids single cell cloning, and screen these pairs with genome-wide CRISPR-

90 romatin immunoprecipitation (ChIP), promoter cloning, and site-directed mutagenesis, real-time quanti

91 g technologies have facilitated the mapping, cloning, and validation of genetic variants underlying t

92 This advance was achieved with a multistep cloning approach and by using DNA dilutions to improve p

93 A positional cloning approach indicates that the mutation in aura is

94 Here we used an expression cloning approach to identify genes in human cancer cells

95 onstitutes a platform for synteny-based gene cloning approaches and can support the forthcoming white

96 multaneously, we established simple one-step cloning approaches for expression of multiple sgRNAs wit

97 Whereas current popular cloning approaches use in vitro assembly of DNA fragment

98 Traditional Map based Cloning approaches, used for the identification of desir

99 approach require manipulation via molecular cloning as well as in vitro transcription.

100 T Motif Family gene 7, HvCMF7) by positional cloning as well as its functional validation based on in

101 toreactive, flow cytometric, and single-cell cloning assays have proven to be critical in deciphering

102 ely decreases the requirements for molecular cloning, because the nucleic acid sequence encoding the

103 occur not only during isolation prior to BAC cloning but also when virus is regenerated.

104 We used a positional cloning-candidate gene approach to identify molecular ba

105 ers, chemical induction, and high-throughput cloning capabilities.

106 e progenitors are characterized by increased cloning capacity, impaired development into mature cells

107 t trial emulation techniques on the basis of cloning, censoring, and weighting, we compared the risks

108 d provide proof of principle for therapeutic cloning combined with cell therapy.

109 ne mapping of quantitative traits, map-based cloning, comparative mapping, and in marker-assisted whe

110 Antibody cloning confirmed elicitation of high levels of somatic

111 quencing (or "Cos-Seq"), based on functional cloning coupled to next-generation sequencing.

112 provide a detailed protocol for the design, cloning, delivery, and utilization of such artificial mi

113 Cloning each GBS into luciferase reporters revealed gluc

114 displayed an altered GSL profile, increased cloning efficiency and intracellular Ca(2+), reminiscent

115 The simplicity, cost effectiveness, and cloning efficiency should promote its routine use, espec

116 The cloning efficiency was similar to that of the commercial

117 detected in 5 out of 8 patients (up to 4.5% cloning efficiency).

118 selectable marker, allowing for selection of cloning events against vector circularization.

119 This study describes the cloning, expression and purification of hypoxanthine-gua

120 After cloning, expression, and purification of the correspondi

121 Following successful cloning, expression, and purification of this region, th

122 egion 3 (CDR3) sequences, a prerequisite for cloning/expression of discovered TCRs.

123 ic and genetic analyses, including map-based cloning, feasible.

124 Here, we report a cloning-free method to target the mouse genome by pronuc

125 Here, we present a rapid and cloning-free mutagenesis technology that can efficiently

126 r this pipeline, including target selection; cloning-free single-guide RNA (sgRNA) synthesis; microin

127 we employ CRISPR/Cas9 technology to build a cloning-free toolkit that addresses commonly encountered

128 Here, we used a "cloning-free" saturation genome editing approach in a di

129 have combined lineage tracing with antibody cloning from single B cells to examine the role of affin

130 om this study lay a solid foundation for the cloning, functional characterization and marker-assisted

131 Cloning, gene regulation, and neuronal proliferation fun

132 ding those interested in structural biology, cloning, glycobiology and chemical biology.

133 We conclude that the original concerns that cloning had caused early-onset OA in Dolly were unfounde

134 Animal cloning has gained popularity as a method to produce gen

135 In tomato (Solanum lycopersicum), molecular cloning has revealed that the underlying genes of natura

136 on holds the potential for optimal molecular cloning, however, current strategies require specialised

137 se resistances in Gy14 and further map-based cloning identified a candidate gene for the resistant lo

138 Map-based cloning identified that the LHR1 gene encodes the polyam

139 cts alongside TIR1, ARF7, and AUX1 Map-based cloning identified TNI as UBP14 Inefficient splicing of

140 AgRenSeq enables R gene cloning in any crop that has a diverse germplasm panel.

141 ool to fully-automate primer design for gene cloning in polyploids, where previously the consensus wi

142 sclerosis has been considered ever since its cloning in the 1990s, but the exact role played by this

143 LUT4) protein in 1988 inspired its molecular cloning in the following year.

144 Current consensus for gene cloning in wheat is to manually perform many steps in a

145 Streptococcus sanguinis, we have developed a cloning-independent methodology, which uses a countersel

146 el fertility, resulting in a final map-based cloning interval of 12 kb.

147 nce of the two oligonucleotides required for cloning into 'B/c' compatible vectors.

148 material, HCMV genomes can be stabilized by cloning into bacterial artificial chromosomes (BACs), an

149 Molecular cloning is a cornerstone of biomedical, biotechnological

150 TPA cloning is scarless and sequence independent.

151 P) mutation on NK cell cytolytic function by cloning it into a lentiviral expression vector prior to

152 een assembled together by modular idempotent cloning, it is unclear if such simplified strategies sca

153 igene expression due to increased labour for cloning, limited vector capacity, requirement of multipl

154 Targeted cloning, mapping, and sequencing of parental and novel t

155 Our in vivo blunt-end cloning method and destabilization-domain-fused Cas9 var

156 Using the efficient fragment exchange (FX) cloning method, the sybody sequences are transferred fro

157 Using a dye dilution-based T cell cloning method, we generated and characterized 24 unique

158 As such, improved cloning methodologies can significantly advance the spee

159 cant advantages over alternative sorting and cloning methods by eliminating the necessity for repetit

160 Traditional cloning methods have limitations on the number of DNA fr

161 f cells and organisms, relying upon cellular cloning methods that remain unchanged for decades, are l

162 ation in molecular biology, from traditional cloning methods to modern synthetic biology and molecula

163 However, a standardized modular cloning (MoClo) system is not yet available for cyanobac

164 We report the full-length cDNA cloning, molecular characterization and functional analy

165 n vitro isothermal reactions are useful when cloning multiple fragments, for site-directed mutagenesi

166 natural competence based large DNA fragment cloning (NabLC) technique was developed, which can move

167 rse SK line, we used it to perform map-based cloning of a major effect quantitative trait locus contr

168 and populations, this study will support the cloning of a major yield QTL on chromosome 3B that is hi

169 We report the cloning of a P. pachyrhizi resistance gene CcRpp1 (Cajan

170 It has been 20 years since the cloning of ATR, the last of the three to be identified.

171 rminants in the blast fungus resulted in the cloning of avirulence genes PWT3 and PWT4, whose gene pr

172 Recent studies reported cloning of candidate genes for several diseases in cucum

173 g annotated genome assemblies and positional cloning of candidate genes.

174 y of these events, however, has hindered the cloning of cells with the desired rearrangement before o

175 ded oligonucleotides for reproducible pooled cloning of CRISPR/Cas9 libraries.

176 rt the first de novo synthesis and cell-free cloning of custom DNA libraries in sub-microliter reacti

177 also used endpoint dilution PCR followed by cloning of env genes to create pseudotyped virus to expl

178 Map-based cloning of fcd1 indicated that this gene encodes the pla

179 Here we report the map-based cloning of Fhb1 from a Chinese wheat cultivar Sumai 3.

180 sed significant progress in fine-mapping and cloning of genes controlling QDR.

181 eloped molecular genomics tool for the rapid cloning of genes in plants.

182 , labor, and time required for synthesis and cloning of gRNAs, allowing generation of CRISPRi librari

183 Conversely, the original cloning of human AC9 reported that AC9 is insensitive to

184 Through systematic cloning of its genes, we identified an Acr solely respon

185 We report here the cloning of Male Sterile23 (Ms23), encoding an anther-spe

186 A major unresolved issue in the cloning of mammals by somatic cell nuclear transfer (SCN

187 The cloning of Ms2 has substantial potential to assemble pra

188 Cloning of multiple genes in a single vector has greatly

189 Since the cloning of NPFFR1 and NPFFR2 in 2000, significant progre

190 Dilution cloning of P. falciparum transfectants showed that indiv

191 The cloning of Pm1a and its identification in a highly rearr

192 Here, we report the map-based cloning of powdery mildew resistance gene Pm24 from Chin

193 n to asexual blood stage culture followed by cloning of recombinant progeny in vitro.

194 This work ushered in the cloning of related proteins, GATA-2-6, with distinct and

195 e the utility of our tool for the positional cloning of resistance genes, we estimated the number of

196 Restriction of the Ps56 genome, cloning of several fragments, and resection of the fragm

197 holipid biosynthesis in Escherichia coli and cloning of several of the genes encoding these enzymes i

198 pproaches because they generally require the cloning of several truncation mutants.

199 ion experiments were performed by sequential cloning of six P. falciparum isolates growing in human e

200 Over the past 25 years, successive cloning of SLC34A1, SLC34A2 and SLC34A3, which encode th

201 sment of their pathogenic potential requires cloning of such antibodies.

202 esses, but a rate-limiting step is often the cloning of targeted genes.

203 The rapid cloning of TdHMA3-B1 rescues a wild beneficial allele an

204 Here we report cloning of the biosynthetic gene cluster for the beta-la

205 g to guide the identification, recovery, and cloning of the cde biosynthetic gene cluster from a soil

206 Cloning of the CER11 gene revealed that it encodes a C-T

207 In this study, map-based cloning of the de locus showed that the determinate grow

208 search on elicitins culminated in the recent cloning of the elicitin response (ELR) cell surface rece

209 It appears that after the initial cloning of the enzymatically active soluble part of P. a

210 Amplification and cloning of the full-length of the sodium channel gene in

211 The original cloning of the gene encoding the intermediate conductanc

212 Here, we report positional cloning of the gene responsible for production of retrov

213 a-informed forward genetics approach enabled cloning of the gl14 gene, which encodes a putative membr

214 The cloning of the H3R cDNA in 1999 by Lovenberg et al. allo

215 Molecular cloning of the Hnrnph1 5' UTR containing all four varian

216 Here, we report the identification and cloning of the M. truncatula NFS2 gene that regulates th

217 We herein report the cloning of the Medicago truncatula NFS1 gene that regula

218 Here, we describe the map-based cloning of the Ms2 gene and show that Ms2 confers male s

219 Here we report the map-based cloning of the Ms2 gene.

220 asochistic endeavor) in 1987, leading to the cloning of the Pemt cDNA.

221 We report the map-based cloning of the powdery mildew resistance allele Pm5e fro

222 typicum riboflavin catabolic strain, and the cloning of the riboflavin catabolic genes.

223 the sigma(1) receptor and discuss the recent cloning of the sigma(2) receptor.

224 Following the cloning of the T cell receptor (TCR), the race was on to

225 Here, we describe the cloning of two paralog genes, H03-IPSE and H06-IPSE, whi

226 The cloning of YrAS2388R will facilitate breeding for stripe

227 Here, we report cloning of ZmNope1 on the basis of synteny with rice.

228 in vitro assembly of DNA fragments, in vivo cloning offers potential for greater simplification.

229 attempts to clone the SMO1 gene by map-based cloning or complementation have failed over many years.

230 were characterized by direct sequencing and cloning; phenotypes were assessed by assays of rod opsin

231 specific T cells of interest for single cell cloning, phenotyping, and transcriptomics.

232 que is based on the previously described TAR cloning procedure developed for isolation of a desirable

233 advantages over alternative methods for all cloning procedures (insertions, deletions, site-directed

234 enzymatic assembly and reduces all molecular cloning procedures to a single-tube, single-step PCR, pe

235 lized bacteria, therefore vastly simplifying cloning procedures.

236 lows unprecedented simplification of complex cloning procedures: five simultaneous modifications of a

237 asmid-based recombination assays and in vivo cloning processes.

238 his poorly understood pathway offers optimal cloning properties (i.e. seamless, directional, and sequ

239 a versatile pipeline enabling parallel gene cloning, protein expression and purification, and in viv

240 ransformation-associated recombination (TAR) cloning protocol, enabling selective isolation of DNA se

241 Nevertheless, methods such as cloning, recombination, and electrospinning have not suc

242 is generally assumed that bacterial in vivo cloning requires Escherichia coli strains with enhanced

243 Here, a single cell cloning revealed that HepAD38 cells, a widely-used HBV-i

244 Using molecular cloning, RT-PCR, Western blotting, immunolocalization an

245 Bisulfite cloning sequencing analysis shows that the paternal alle

246 pared the results with those obtained by PCR-cloning-sequencing (PCR-CS).

247 sequencing, 16S rRNA gene pyrosequencing and cloning/sequencing hgcAB gene products.

248 y-based unbiased analysis followed by T cell cloning, several findings were made.

249 esulting F(2) progeny followed by positional cloning showed that resistance to an isolate of A. candi

250 Antibody cloning shows that donors with high ZIKV neutralizing an

251 of medical interest, it has eluded molecular cloning since its discovery, and the gene that codes for

252 mple involves dsRNA produced by the multiple cloning site (MCS) of L4440, which shares complementary

253 part of a sequence- and ligation-independent cloning (SLIC) reaction and then transformed into Escher

254 The initial cloning stage can take 5-7 d, and the subsequent protein

255 lity to generate such constructs in a single cloning step.

256 oratory equipment or techniques, or multiple cloning steps.

257 or intense and require multiple ligation and cloning steps.

258 and PCR-independent Golden Gate and Gateway cloning strategies, which will facilitate transcriptiona

259 Using a positional cloning strategy and a retrotransposon insertion line, w

260 t inhibition of transcription and the simple cloning strategy described in this work, CRISPR interfer

261 We also developed a lentiviral vector and cloning strategy to generate high-complexity pooled dual

262 es and five genes supported by previous gene cloning studies in maize, rice, and Arabidopsis.

263 Map-based cloning studies revealed that CTL1 mutations alter the i

264 were supported by both QTL analyses and gene cloning studies.

265 y genetic variants in post-GWAS or post-QTL- cloning studies.

266 The modular cloning system developed in this study allows for the ef

267 The cloning system is provided under an OpenMTA license for

268 GoldenBraid is a rapid, modular, and robust cloning system used to assemble and combine genetic elem

269 In this work, a complete modular chloroplast cloning system, MoChlo, was developed and validated for

270 A common strategy is to develop cloning technologies aimed at increasing the fixation ti

271 Here we apply single-cell cloning technologies to lung tissue of patients with and

272 erial dilutions of CD4(+) T cells and T cell-cloning technologies, we are able to demonstrate that de

273 we utilized a high-throughput single B cell cloning technology to longitudinally track the human B c

274 artificial minichromosome was constructed by cloning the centromere DNA of the budding yeast Saccharo

275 ated with a known strawberry mutation before cloning the dek mutants, thereby enabling phenotypic ana

276 Cloning the sigma2 receptor resolves a longstanding myst

277 Cloning the taurine PRDM9 gene, which is the common form

278 last 30 years, with the advent of positional cloning, the human genome project, solid-state genotypin

279 ed from the oral metagenome, was verified by cloning them upstream of a gusA reporter, proving they c

280 . coli self-assembly have greatly simplified cloning, these have not yet been harnessed for the high-

281 We employed molecular cloning to examine how PTEN's stability, subcellular loc

282 ulked segregant RNA sequencing and map-based cloning to identify the genetic lesion underlying a rece

283 s were used for somatic nuclear transfer and cloning to produce a bovine fetus homozygous for the Q(5

284 y used for LLHR, expanding the Rec/ET direct cloning toolbox and providing the possibility for rapid

285 mplified protocols and minimal requirements, cloning using in vivo DNA assembly in E. coli has the po

286 In addition to cloning variants of previously characterized genes, spec

287 hiazine [DIAT], N-acetyl DIAT & epoxide) and cloning was attempted in a number of patients.

288 Genome editing followed by reproductive cloning was previously used to produce two hornless dair

289 Using map-based cloning we positioned the her2 mutation to the At5g63620

290 Using map-based cloning, we delimited a candidate region including two l

291 After the HAT-selection and cloning, we established nine hybridoma clones secreting

292 Using expression cloning, we identified an accessory protein, 17 kDa memb

293 By using single-cell cloning, we identified genes that are associated with hi

294 Using mapped-based cloning, we identify the RAA7 locus, which encodes a pio

295 Using map-based cloning, we localized TdDof to within a physical interva

296 s, followed by somatic cell nuclear transfer cloning, which can be inefficient.

297 Rapid and reliable network cloning with single-synapse precision is thus theoretica

298 tive polyploid primer-design method for gene cloning, with no need for researchers to download softwa

299 antibody variable genes and accelerates the cloning workflow.

300 ylyi ADP1 to create easy and rapid molecular cloning workflows, including a Cas9-based single-step ma