ライフサイエンスコーパス: closed-circular

1 also show that SWI-SNF is able to remodel a closed circular array in the absence of topoisomerase I,

2 We demonstrate here that a remodeled closed circular array will revert toward its original to

3 d by an effect of RSC on the supercoiling of closed, circular arrays of nucleosomes.

4 ecificity in an end-independent manner using closed circular BUR-containing DNA substrates.

5 However, covalently closed circular (CCC) and nicked open circular DNA are n

6 ractions also negatively regulate covalently closed circular (CCC) DNA formation, which occurs after

7 Hepatitis B virus (HBV) covalently closed circular (CCC) DNA functions as the only viral te

8 and is sustained by the episomal covalently closed circular (CCC) DNA in the nuclei of infected hepa

9 Covalently closed circular (CCC) DNA is present indicating that, in

10 nthesis of hepadnaviral RNAs is a covalently closed circular (ccc) DNA located in the nucleus of the

11 cation of HBV genome organized as covalently closed circular (ccc) DNA minichromosome.

12 TANCE The hepatitis B virus (HBV) covalently closed circular (CCC) DNA, by serving as the viral trans

13 ell nucleus for conversion to the covalently closed circular (CCC) DNA, the template for viral transc

14 equired for its conversion to the covalently closed circular (CCC) DNA, the viral episome serving as

15 thesis of hepatitis B virus (HBV) covalently closed circular (ccc) DNA, we developed a cell-based ass

16 incoming RC-DNA is converted into covalently closed circular (ccc) DNA, which serves as a viral persi

17 c mature nucleocapsid (NC) to the covalently closed circular (CCC) DNA, which serves as the template

18 RC DNA needs to be converted to covalently closed circular (CCC) DNA, which serves as the template

19 the host cell nucleus to form the covalently closed circular (CCC) DNA, which sustains viral replicat

20 c CTDs (DCTDs) decreased the DHBV covalently closed circular (CCC) DNA.

21 regular or synthetic AP site on a covalently closed circular (ccc) duplex plasmid molecule or a long

22 be because it fails to eliminate covalently closed circular (CCC) HBV DNA from the nuclei of infecte

23 lly active at this time or if the covalently closed circular (CCC) replicative intermediate of HBV DN

24 The highly structured (64% GC) covalently closed circular (CCC) RNA (220 nt) of the virusoid assoc

25 known replicators that consist of covalently closed circular (ccc) RNA molecules between 200 and 400

26 Viroids and viroid-like covalently closed circular (ccc) RNAs are minimal replicators that

27 pregenome, are transcribed from a covalently closed circular (ccc) viral DNA that is found in the nuc

28 arance of nonencapsidated nuclear covalently closed circular (CCC) viral genomic DNA.

29 Hepatitis B virus (HBV) covalently closed circular (ccc)DNA is the key genomic form respons

30 tions of intrahepatic WHV RNA and covalently closed circular (ccc)WHV-DNA levels also were observed.

31 open-circular (oc, "nicked"), and covalently closed circular (ccc, "supercoiled") form.

32 ith transcription of intrahepatic covalently closed circular (cccDNA), which is an important target f

33 he CRISPR-based system to produce covalently closed circular (cccDNA)-like extrachromosomal DNAs desc

34 three of these clamps have been shown to be closed, circular complexes.

35 in which the protein induces nearly the same closed circular configuration point to the statistical a

36 atin, which might account for the covalently closed circular conformation of quiescent HCMV genomes.

37 electroporating in the presence of 50 microg closed-circular construct.

38 , followed by purification of the covalently closed circular constructs.

39 f the conversion of nicked plasmids from the closed circular damaged plasmid substrate; the inhibitio

40 itro results in the generation of covalently closed circular daughter molecules, indicating that the

41 lease V in about one-half (46 +/- 9%) of the closed circular daughter molecules.

42 HB) because they do not eliminate covalently closed circular deoxyribonucleic acid, the stable replic

43 ermore, the CDM mutations blocked covalently closed circular DNA (CCC DNA) formation during infection

44 ed to be used as an indicator for covalently closed circular DNA (cccDNA) (HBV's minichromosome in he

45 g in more nuclear-localized viral covalently closed circular DNA (cccDNA) and boosting transcription.

46 n depends on the establishment of covalently closed circular DNA (cccDNA) and can be either transient

47 licative intermediates, including covalently closed circular DNA (cccDNA) and Dane particles, were de

48 HBV-infected organoids produced covalently closed circular DNA (cccDNA) and HBV early antigen (HBeA

49 rface antigen (HBsAg) levels from covalently closed circular DNA (cccDNA) and the integrated genome c

50 he hepatitis B virus (HBsAg) from covalently closed circular DNA (cccDNA) and the integrated genome,

51 r to monitor the intrahepatic HBV covalently closed circular DNA (cccDNA) and to define meaningful tr

52 At the same time, covalently closed circular DNA (cccDNA) and viral mRNA levels both

53 plicative intermediates including covalently closed circular DNA (cccDNA) are present.

54 relaxed circular DNA (rcDNA) and covalently closed circular DNA (cccDNA) became detectable sequentia

55 treatment does not affect initial covalently closed circular DNA (cccDNA) conversion but inhibits the

56 B (APOBEC3B; A3B), and subsequent covalently closed circular DNA (cccDNA) decay.

57 HBV is eradication of the stable covalently closed circular DNA (cccDNA) form of the viral genome, w

58 ion is eradicating or silencing the covalent closed circular DNA (cccDNA) form of the viral genome.

59 n assembly, capsid uncoating, and covalently closed circular DNA (cccDNA) formation.

60 Covalently closed circular DNA (cccDNA) forms the basis for replica

61 hromosome consisting of the viral covalently closed circular DNA (cccDNA) genome and host histones.

62 pecifically identifies methylated covalently closed circular DNA (cccDNA) in human liver tissue.

63 pies, lies in the accumulation of covalently closed circular DNA (cccDNA) in nuclei of infected cells

64 is subsequently converted into a covalently closed circular DNA (cccDNA) in the host cell nucleus.

65 mation of an intranuclear pool of covalently closed circular DNA (cccDNA) in the liver.

66 virus (HBV) is caused by the persistence of closed circular DNA (cccDNA) in the nucleus of infected

67 ) genome that is converted into a covalently closed circular DNA (cccDNA) in the nucleus of the infec

68 Quantification of intrahepatic covalently closed circular DNA (cccDNA) is a key for evaluating an

69 HBV covalently closed circular DNA (cccDNA) is a major obstacle for a c

70 Hepatitis B virus (HBV) covalently closed circular DNA (cccDNA) is an episomal minichromoso

71 itis B virus (HBV) infection, HBV covalently closed circular DNA (cccDNA) is formed in the cell nucle

72 cccDNA biogenesis.IMPORTANCE The covalently closed circular DNA (cccDNA) is the persistent form of t

73 mation and persistence of the HBV covalently closed circular DNA (cccDNA) is the root cause of HBV ch

74 ) and subgenomic RNA from the HBV covalently closed circular DNA (cccDNA) minichromosome, both in cul

75 leus of infected hepatocytes as a covalently closed circular DNA (cccDNA) molecule, a reservoir of HB

76 criptional template, a long-lived covalently closed circular DNA (cccDNA) molecule, is degraded noncy

77 k hepatitis virus (WHV) contained covalently closed circular DNA (cccDNA) molecules with deletions an

78 Covalently closed circular DNA (cccDNA) of hepadnaviruses exists as

79 Covalently closed circular DNA (cccDNA) of hepatitis B virus (HBV)

80 HBV covalently closed circular DNA (cccDNA) plays an essential role in

81 The persistence of covalently closed circular DNA (cccDNA) poses a major obstacle to c

82 thesis of hepatitis B virus (HBV) covalently closed circular DNA (cccDNA) requires the removal of the

83 control the nuclear pool of viral covalently closed circular DNA (cccDNA) transcriptional template of

84 utants were competent to form the covalently closed circular DNA (cccDNA) via the intracellular ampli

85 ed that both encapsidated DNA and covalently closed circular DNA (cccDNA) were turned over independen

86 plication-derived RNAs (rd-RNAs), covalently closed circular DNA (cccDNA), and core-associated HBV DN

87 e of the hepatitis B virus (HBV), covalently closed circular DNA (cccDNA), has been difficult to stud

88 eve because of the persistence of covalently closed circular DNA (cccDNA), HBV-DNA integration into t

89 istence of the viral episome, the covalently closed circular DNA (cccDNA), in hepatocytes.

90 rsistence of the HBV episome, the covalently closed circular DNA (cccDNA), in the nuclei of infected

91 orms a stable minichromosome, the covalently closed circular DNA (cccDNA), in the nucleus of infected

92 use the transcriptional template, covalently closed circular DNA (cccDNA), is long lived in infected

93 but not to levels of intrahepatic covalently closed circular DNA (cccDNA), plasma pregenomic RNA, or

94 in the intranuclear pool of viral covalently closed circular DNA (cccDNA), resulting in a transient e

95 lead to the methylation of viral covalently closed circular DNA (cccDNA), resulting in its transcrip

96 ause it does not directly affect nuclear HBV closed circular DNA (cccDNA), the genomic form that temp

97 ation, including the formation of covalently closed circular DNA (cccDNA), the molecular basis for es

98 Covalently closed circular DNA (cccDNA), the nuclear form of hepati

99 replication and formation of new covalently closed circular DNA (cccDNA), the viral transcriptional

100 Ag) as surrogates of silencing of covalently closed circular DNA (cccDNA), to characterize this disso

101 eradicate or inactivate the viral covalently closed circular DNA (cccDNA), which is a stable episomal

102 V infection is the persistence of covalently closed circular DNA (cccDNA), which is traditionally con

103 The HBV genome persists as covalently closed circular DNA (cccDNA), which serves as the templa

104 he intrahepatic pool of the viral covalently closed circular DNA (cccDNA), which serves as the transc

105 lower levels of intrahepatic WHV covalently closed circular DNA (cccDNA).

106 rting that transcription was from covalently closed circular DNA (cccDNA).

107 rcular DNA that transforms into a covalently closed circular DNA (cccDNA).

108 to a persistent viral genome, the covalently closed circular DNA (cccDNA).

109 ear template for HBV replication, covalently closed circular DNA (cccDNA).

110 of HBV replication, including the covalently closed circular DNA (cccDNA).

111 in the formation of hepadnavirus covalently closed circular DNA (cccDNA).

112 number of episomal viral genomes [covalently closed circular DNA (cccDNA)] in the nuclei of infected

113 Closed circular DNA (form I) is converted into nicked ci

114 ifically target hepatitis B virus covalently closed circular DNA (HBV cccDNA), the episomal form of t

115 ularized HBV DNA (which resembles covalently closed circular DNA [cccDNA]).

116 eplication is due to a less rapid covalently closed circular DNA amplification, leading to lower vire

117 nctional cure are the presence of covalently closed circular DNA and ineffective/exhaustive immune sy

118 rhaps complete cure (clearance of covalently closed circular DNA and integrated HBV DNA).

119 Messenger RNAs were derived from covalently closed circular DNA and integrated HBV DNA.

120 for clearance or silencing of all covalently closed circular DNA and integrated HBV DNA.

121 strains as both low-copy-number, covalently closed circular DNA and tandemly duplicated, chromosomal

122 ar form, it would be adventitious to amplify closed circular DNA as closed circular molecules.

123 surface antigen translation, and covalently closed circular DNA biogenesis.

124 f significantly higher amounts of covalently closed circular DNA compared with wild-type HBV replicat

125 Closed circular DNA constructs containing a single 8-oxo

126 Closed circular DNA constructs containing a single TG at

127 We found that although viral covalently closed circular DNA declined 20- to 100-fold, integrated

128 us, chemotherapeutic clearance of covalently closed circular DNA did not involve the replacement of t

129 (CAG)n and (CTG)n extrusions in relaxed closed circular DNA do in fact support MutSbeta-, replic

130 complex does not assemble beta onto relaxed closed circular DNA even at low ionic strength.

131 ed without amplification by using covalently closed circular DNA extracted from the liver of an infec

132 binding, taurocholate uptake, HBV covalently closed circular DNA formation, and expression of all HBV

133 viral replication by clearance of covalently closed circular DNA from infected hepatocytes.

134 ee 3' end, which would nevertheless preserve closed circular DNA in either single-stranded (SS) or do

135 could generate a small amount of covalently closed circular DNA in LMH cells, a chicken hepatoma cel

136 able to introduce positive supercoils into a closed circular DNA in the presence of bacterial or euka

137 cells or permanently silence the covalently closed circular DNA in those cells, and that will stimul

138 and the resulting apparent unwinding of the closed circular DNA is used to calculate both ligand unw

139 n the transcriptional template or covalently closed circular DNA level.

140 Viral antigens and covalently closed circular DNA levels in liver samples were signifi

141 tion of virus entry since initial covalently closed circular DNA levels were not decreased in IFN-tre

142 f inducing and/or maintaining HBV covalently closed circular DNA methylation, resulting in transcript

143 termediate produced from a stable covalently closed circular DNA molecule.

144 We have isolated covalently closed circular DNA molecules carrying hybrid pilin loci

145 diates, no effect on the level of covalently closed circular DNA or HBV transcripts was observed at l

146 atocyte proliferation and loss of covalently closed circular DNA or its equivalent, possibly assisted

147 ize intermediates of in vitro replication of closed circular DNA plasmids.

148 inding assays, and topological analyses with closed circular DNA show that the properties of multipro

149 Solution studies with closed circular DNA show this compound to be a bisinterc

150 Transcription of closed circular DNA templates in the presence of DNA gyr

151 on histone-DNA interactions were studied on closed circular DNA that was either moderately or positi

152 ive subtype in suppression of HBV covalently closed circular DNA transcription and HBV e antigen/HBV

153 cation (LDA), for selective amplification of closed circular DNA using sequence-specific primers.

154 cation (LDA), for selective amplification of closed circular DNA using sequence-specific primers.

155 ic RNA and efficient synthesis of covalently closed circular DNA were detected after infection with t

156 Reductions of covalently closed circular DNA were seen in hepatocyte-like cells.

157 complete cure (ie, eradication of covalently closed circular DNA) of CHB, several challenges in basic

158 igated using plasmid DNA, relaxed covalently closed circular DNA, and linear duplex DNA as substrates

159 ed RNAs associated primarily with covalently closed circular DNA, consistent with this structure bein

160 molar input ratio) in supercoiling relaxed, closed circular DNA, in inducing ligase-mediated circula

161 d Elg1-RFC could remove PCNA from covalently closed circular DNA, indicating that PCNA unloading occu

162 oposed to reflect the activity of covalently closed circular DNA, may improve the ability to predict

163 as replicative intermediate DNA, covalently closed circular DNA, pregenomic RNA, and the percentage

164 levels and impaired production of covalently closed circular DNA, the template for DHBV gene expressi

165 ecause of their lack of effect on covalently closed circular DNA, the template of viral transcription

166 for reversibly binding DNA ligands that uses closed circular DNA, topoisomerase I (Topo I), and two-d

167 ses the transcriptional template, covalently closed circular DNA, was formed by circularization of li

168 ) and surface (HBs) antigens, and covalently closed circular DNA, was observed in HUHEP and HIS-HUHEP

169 of 0.7 to 3.5 kb from the 3.2-kb covalently closed circular DNA, with the 2.1-kb RNA being most abun

170 the presence of E. coli topoisomerase I and closed circular DNA.

171 additionally includes loss of HBV covalently closed circular DNA.

172 nstructs, or constructs mimicking covalently closed circular DNA.

173 C DNA by removing all DNA species other than closed circular DNA.

174 s, proteins, replicative DNA, and covalently closed circular DNA.

175 of HBsAg, HBeAg, and intrahepatic covalently closed circular DNA.

176 not inhibited by 1 microg RNA or covalently closed circular DNA.

177 ed with foreign, single-stranded, covalently closed, circular DNA molecules identical in length to th

178 HBV replicates via an episomal covalently-closed-circular DNA (cccDNA) and integrated viral DNA fr

179 ing treatment from the stable HBV covalently-closed-circular DNA (cccDNA).

180 We have exploited the observation that closed-circular DNA containing an inverted repeat can re

181 Wadjet specifically cleaves closed-circular DNA in a reaction requiring ATP hydrolys

182 e fragments to double-sided adapters to form closed-circular DNA molecules.

183 the presence of DNA nicks, and can occur on closed-circular DNAs in the absence of topoisomerases.

184 l spectrum generated during transcription of closed circular double-stranded DNA indicates that wild

185 gle-stranded DNA and on actively transcribed closed circular double-stranded DNA.

186 Secondly, the binding of RHA to closed circular dsDNA stimulates the relaxation reaction

187 ng linear RecA-ssDNA filament and covalently closed circular duplex DNAs is promoted by supercoiling

188 e lesion during bidirectional replication of closed circular duplex molecules carrying the SV40 origi

189 turbations induced when echinomycin binds to closed circular duplex pBR322 DNA were also investigated

190 convert its linear genome to the covalently closed circular episomal form in which it persists in pr

191 ic of latent herpesviruses-large, covalently closed, circular episomes.

192 conversion of nicked circular plasmid to the closed circular form by cell extracts filling the repair

193 or each HBV DNA species, with the covalently closed circular form of HBV DNA being the most resistant

194 ote limited excision to produce a covalently closed circular form of the transposon, showing that Tn

195 competent form of many DNA molecules is the closed circular form, it would be adventitious to amplif

196 inant form of VS RNA observed in vivo is the closed circular form, though minimal VS ribozyme self-cl

197 eplication and causes a complete loss of the closed-circular form of EBV episomes in latently infecte

198 nd a heavy (H) strand, exists as a multicopy closed circular genome within the mitochondrial matrix.

199 Assembly and comparison of 21 ERBM closed circular genomes identified five as members of a

200 lopment of treatment to eradicate covalently closed circular HBV DNA, and development of immunotherap

201 and relaxed circular compared to covalently closed circular HBV DNA.

202 The corresponding closed circular helicates that are formed from a diamine

203 When present in covalently closed circular heteroduplexes or heteroduplexes contain

204 Helraiser transposition generates covalently closed circular intermediates, suggestive of a replicati

205 ition of a bat Helitron generates covalently closed circular intermediates.

206 tochondrial DNA (mtDNA) is a double-stranded closed circular molecule of about 16 kb coding for 37 ge

207 synthesis products, i.e., newly synthesized closed circular molecules carrying the photoproduct, wer

208 ventitious to amplify closed circular DNA as closed circular molecules.

209 lly all the plastid DNA comprises covalently closed circular monomers, together with a tiny minority

210 A closed circular neck undergoes fission, thereby generati

211 terations cause changes in the topology of a closed circular nucleosomal array that persist after rem

212 If closed circular pBR322 DNA is present when the peroxo-va

213 transcription system was a large covalently, closed circular plasmid (8.9 kb).

214 ty was further confirmed by using covalently closed circular plasmid DNA as a substrate.

215 troduce superhelical tension into covalently closed circular plasmid DNA is stimulated by Rad51.

216 oth orientations, DpnI-resistant, replicated closed circular plasmid DNA was sensitive to nicking by

217 ive ligand PIPER can unwind double-stranded, closed circular plasmid DNA, as determined by a topoisom

218 to the major fluorescent product, covalently closed circular plasmid DNA.

219 functional for initiating DNA synthesis on a closed circular plasmid.

220 ) stimulated the cleavage of double-stranded closed-circular plasmid DNA.

221 The method uses closed circular (plasmid) DNA and can separate single-st

222 ion produces linear products, and as of yet, closed circular products have not been possible.

223 econd, we measured the change in topology of closed circular relaxed DNA following binding of HU.

224 mants with extrachromosomal plasmid DNA when closed circular, replicative plasmid carrying an uptake

225 PO(4) single-strand RNA to form a covalently closed circular RNA molecule through ligase-adenylylate

226 The HDV genome is a closed circular RNA of about 1,700 bases which is replic

227 Covalently closed circular RNA transcripts are prevalent in eukaryo

228 ar ligation of single-stranded RNA to form a closed circular RNA via covalent ligase-AMP and RNA-aden

229 CircRNAs are covalently closed, circular RNA molecules that typically comprise e

230 C are defective phages that contain a novel closed circular single-stranded DNA and that this DNA wa

231 indicated that pyocin C particles contained closed circular single-stranded DNA, approximately 4.0 k

232 , it exists as a protein capsid to protect a closed circular, single-stranded DNA (ssDNA) genome.

233 where the linear dsDNA is used to mimic the closed circular, ssDNA in M13 phage, upon removal of the

234 of LH1 complexes thought to exist as a large closed circular structure only in the latter strain.

235 erent sites, and loading PCNA onto a nicked, closed circular substrate with a unique Hx residue enhan

236 ear non-homologous DNA ends repaired to form closed-circular supercoiled monomers, are joined without

237 ogy that can efficiently generate covalently-closed-circular-synthesized (3Cs) CRISPR/Cas gRNA reagen

238 rom Anabaena was used to generate covalently closed circular trans-acting ribozymes in Escherichia co

239 Excision and formation of a covalently closed circular transposon molecule are required for con

240 ermediates from the cytoplasm and covalently closed circular viral DNA from the nucleus of infected c