ライフサイエンスコーパス: coculture

1 lls and leukocytes were studied alone and in coculture.

2 trifugation, 97% Cd(II) was removed from the coculture.

3 vity, a difference not seen in 2-dimensional coculture.

4 expressing mice induce motor neuron death in coculture.

5 uting the specimen in broth and by Vero cell coculture.

6 significantly lower in L. rostrata cells in coculture.

7 lated from an environmental sample by amoeba coculture.

8 a) by microglia when the two cell types were cocultured.

9 , DCs and autologous naive CD4+ T cells were cocultured.

10 invasion of tumor cells when both cells were cocultured.

11 origenic function in primary B cell lymphoma cocultures.

12 ted phagocytosis of CD47(-/-) tumor cells in cocultures.

13 s the primary mode of communication in these cocultures.

14 hagocytosis of tumor cells by macrophages in cocultures.

15 gnificant increase in PTEC necrosis in these cocultures.

16 egs from DeltaDC mice was impaired in T cell cocultures.

17 f inflammatory macrophages in macrophage-IEC cocultures.

18 d into the vaginal epithelial cells in their cocultures.

19 and NET/human aortic endothelial cell (HAEC) cocultures.

20 ) cell differentiation and cTFH/naive B-cell cocultures.

21 evidence of prominent occludin expression in cocultures.

22 ML, and that IL8 was increased in AML/BM-MSC cocultures.

23 factors on the chondrogenesis of MSC and AC cocultures.

24 s of such networks in organoids and organoid cocultures.

25 ma and in vitro using myeloma cell-adipocyte cocultures.

26 ed anti-inflammatory cytokines in macrophage cocultures.

27 Contact-dependent transmission between cocultured 293T and MT-4 cells is higher than in cocultu

28 Coculturing a macrophage cell line with hypoxia-treated

29 AD1 in metastatic cells by primary glia cell coculture abolished the capacity of metastatic cells to

30 We further show that cocultured allogeneic T cells kill Atg16L1-mutant intest

31 Coculture analysis indicated that CCR2-induced stromal r

32 ll number and neurites, as shown by indirect coculture and direct addition of PMSC-conditioned medium

33 to phagocytosis of stimulation beads used in cocultures and absent when using soluble or coated Abs.

34 Transwell coculturing and conditioned media treatments were select

35 lity to drive memory T cell proliferation in coculture, and skewed CD4(+) T cells to express an incre

36 s using single-cell RNA sequencing, in vitro coculture, and subcutaneous injection of MSCs and myelom

37 to two different types of samples, bacteria cocultures, and brain tissue sections.

38 naive T-cell differentiation, B-cell/T-cell cocultures, and regulatory T-cell suppression.

39 gically relevant extracellular gradients and coculture architectures.

40 520 compounds on a transendothelial invasion coculture assay.

41 Strikingly, coculture assays with primary T cells showed that macrop

42 artially inhibit angiogenesis in organotypic coculture assays, and this is reversed by treatment with

43 to increase regulatory T cells (Treg) during coculture assays, features that cannot be captured by us

44 HTRF) assay and PD-1 signaling in cell-based coculture assays.

45 mentally confirm this prediction in pairwise cocultures assembled from a diverse set of species and s

46 ll, human lung fibroblast, and U937 monocyte cocultures (at air-liquid interface) displayed similar C

47 rapa L. were performed with and without the coculture bacteria.

48 Proteomic and metabolomic analyses of the cocultured bacteria revealed that H(2)S and SA productio

49 We found that in SMC and EC contact cocultures, BMPR2 (bone morphogenetic protein receptor 2

50 o formed in the fibrin-Matrigel mixed gel by coculturing brain microvascular endothelial cells (BMECs

51 We cocultured breast cancer and patient-derived fibroblast

52 Here, we present a bacteria-in-spheroid coculture (BSCC) platform that simultaneously tests host

53 spheroids retained ER protein expression in cocultures but acquired redundant compensatory signals e

54 py sensitivity is restored in the fibroblast cocultures by combination treatment with inhibitors of M

55 sed B cells from NP or tonsil, or after ILC2 coculture, by flow cytometry.

56 , the ratio of stromal and cancer cells in a coculture can be used to modulate the expression of the

57 uman cell lines decreased CD80 expression on cocultured CD80(+) cells, with restoration upon CTLA-4 b

58 ed stromal fibroblasts (CAFs) derived from a coculture cell model and clinical patient samples, we de

59 ted fibroblasts increased IL23 production in cocultured cervical cancer-instructed mDCs, which mediat

60 and interactions within constructed defined cocultures comprising the methanogen Methanoculleus bour

61 When compared to aerobic coculture conditions, the establishment of a translumina

62 s were treated in monoculture and cumulative coculture conditions.

63 au can be driven toward LLPS under live cell coculturing conditions with rationally chosen experiment

64 Stromal coculture did not prevent leukemia cell cycle activity,

65 ontrast, cFb-conditioned medium or transwell coculture did not significantly increase cardiomyocyte b

66 n of IL-6 by its blocking antibody in BMM-OB cocultures diminishes the increase of osteoclastogenesis

67 In coculture, E. timonensis promoted the complete l-carniti

68 explained by regulation of E-selectin on the cocultured EC.

69 Remarkably, coculturing embryonic stem cells (ESCs) and trophoblast

70 ociation and invasion of cancer cells toward cocultured endothelial cells.

71 Coculturing endothelial cells with astrocytes yielded th

72 man monocytes and mouse macrophages in tumor cocultures exhibited significantly different FAD mean li

73 rons only from the same region in mismatched cocultures, exhibiting region-matched astrocyte to neuro

74 mediated increase of cancer stemness both in coculture experiments and in mice.

75 Mechanistically, coculture experiments of tumor organoids and CAFs showed

76 Coculture experiments revealed T(FH)17 cells as primaril

77 Coculture experiments showed that IL-33-stimulated mast

78 In vitro coculture experiments showed that MSCs and ECFCs induced

79 iation and response to chemotherapy, various coculture experiments were performed.

80 Neutrophils were used as APCs in coculture experiments with autologous HLA-DR-restricted

81 rganoids isolated from the murine stomach in coculture experiments with live bacteria mimicking the i

82 In coculture experiments, sorted pTfh did not support the B

83 ntibodies by memory B cells was evaluated in coculture experiments.

84 recapitulated in 3-dimensional organoids and coculture experiments.

85 mice stimulated collagen gene expression by cocultured fibroblasts.

86 The same cocultures formed xenograft tumors of significantly redu

87 f these genes in primary neuronal-microglial cocultures from KCNH2-3.1 mice impairs synapse formation

88 C and MM cells; and importantly, that pDC-MM coculture further increases ENO1 expression in both MM c

89 Here, we report that AML-MSC cocultures greatly increase Cox-2 expression in MSC and

90 These Giardia cocultures grew better than nonconditioned trophozoites,

91 Coculture groups demonstrated higher type II collagen ex

92 pertrophic phenotype was also limited in the coculture groups.

93 We established a novel rosetting model by coculturing HLA-II-matched peripheral blood mononuclear

94 V) transfer blocks the synergistic effect of coculture, identifying EVs as the primary mode of commun

95 In cocultures, IL-33-induced Muc2 expression is dependent o

96 und that bacterial invasion of CRC cells and cocultured immune cells induced a differential cytokine

97 scaffold, and the resulting muscle tissue is cocultured in situ with a neural cluster.

98 When platelets were cocultured in the same compartment with lymphocytes, we

99 ally regulate IL23 and IL12 in DC fibroblast cocultures in an IL6/C/EBPbeta/IL1beta-dependent manner,

100 romoted angiogenesis in CAF-endothelial cell cocultures in vitro.

101 DIET have primarily been studied in defined cocultures in which Geobacter species are one of the DIE

102 on lung cancer biology by studying in vitro cocultures, in vivo mouse models, and human lung cancer

103 In addition, IFNgamma blockade in T cell/VEC coculture increased VEC proliferation and VEGF-A protein

104 We find that in a two-species coculture, increasing mortality favors the faster grower

105 Flow cytometry, ELISAs, cocultures, intracellular staining and suppression assay

106 Moreover, when we cocultured K279a with strains of Pseudomonas aeruginosa,

107 Cocultured keratinocytes are essential for induction of

108 RSV infection of BEC/HLF cocultures led to decreased hyaluronidase expression by

109 Coculturing lung cancer cells with Th9/Th17 cells or exp

110 We cocultured MAIT cells and human primary proximal tubular

111 ffectively discriminates cancer cells in the coculture media.

112 mined morphology and functionality, and used coculture methods, high-content microscopy, and RNA sequ

113 GFs were also significantly increased by the coculturing methods.

114 reover, in pHERV-W ENV-stimulated myelinated cocultures, microglia were found to structurally damage

115 We developed an enteroid-immune cell coculture model to determine the mechanism through which

116 HS27a cells were functionally evaluated in a coculture model with ALL targets.

117 In a three-dimensional coculture model, AES-135 kills low-passage patient-deriv

118 endometrial stromal cell proliferation in a coculture model.

119 elial cell (BEC)/human lung fibroblast (HLF) coculture model.

120 and IL-6) expression using an MSC-macrophage coculture model.

121 iated dysfunction with disease; however such coculture models have randomly oriented myotubes with im

122 Using our coculture models of patient autologous pDC-T-NK-MM cells

123 at intercellular mRNA transfer occurs in all coculture models tested (e.g., between primary cells, im

124 Using tissue and coculture models, we found that myofibroblasts and the f

125 trocytes and abrogates their toxicity toward cocultured motor neurons.

126 Altogether, this study demonstrates that coculturing MSCs with ACs can greatly enhance the chondr

127 The binary cocultures obtained could be stably passaged and studied

128 Coculture of bone marrow-derived macrophages with ERCs f

129 Coculture of cells facilitated transition, migration, an

130 everal recent studies have demonstrated that coculture of chondrocytes (CHs) with bone marrow-derived

131 Here we show that coculture of control astrocytes with neurons enhances ne

132 Coculture of CTLA-4(+) CLL cells with CD80-GFP(+) cell l

133 In vitro, only the coculture of DCs with T cells or their supernatants resu

134 PE toxicity that was induced by NaIO3Ex vivo coculture of gammadelta T cells with RPE explants activa

135 MP-2 expression was recently observed in the coculture of human gingival fibroblasts (HGFs) and U937

136 A coculture of human neutrophils preactivated with 50 uM N

137 Coculture of ICAM-1(-/-) neutrophils or wild-type (WT) n

138 Here, we show the extended coculture of living human intestinal epithelium with sta

139 n mice subjected to U-IRI and in vitro using coculture of macrophages and tubular cells.

140 valuated by transwell migration assay and 2D coculture of MSCs with human CD34+ HSCs.

141 We found that coculture of murine bone marrow cells with bladder tumor

142 Here, we examined the effects of coculture of NTHI with human airway epithelial cells and

143 The coculture of platelets with lymphocytes in the presence

144 d in intestinal epithelium dysfunction using coculture of primary endothelial cells and intestinal T8

145 Our 3D kidney tissue allows for coculture of proximal tubule epithelium and vascular end

146 Coculture of sorted, HIV-infected CD4(-) (siCD4(-)) T ce

147 Coculture of T cells with CTLA-4(+) CLL cells decreased

148 Coculture of the AML cell lines MOLM-4, THP-1 or primary

149 Coculture of the osteotropic PC-3M-Pro4Luc2 PCa cells wi

150 A coculture of three yeast strains, each harboring a diffe

151 Coculture of tumor organoids with iCAFs resulted in sign

152 ltured 293T and MT-4 cells is higher than in cocultures of 293T with most other T-cell lines tested,

153 Moreover, in cocultures of bone marrow adipocyte and osteoblast proge

154 crease of osteoclast (OC) differentiation in cocultures of bone marrow macrophages/monocytes (BMMs) a

155 In cocultures of CD4(+) T cells with S. aureus-infected DCs

156 Cocultures of cervical cancer-instructed mDCs and cervic

157 cell proliferation and IFN-gamma release in cocultures of DC-PBMC.

158 cal, molecular, and functional approaches in cocultures of epidermal keratinocytes and sensory neuron

159 Cocultures of human NK cells and endothelial cells confi

160 en primary cells, immortalized cells, and in cocultures of immortalized human and murine cells).

161 on of cytokines produced by myeloid cells to cocultures of LACC1-deficient myeloid cells and wild-typ

162 eight and concentration on chondrogenesis of cocultures of mesenchymal stem cells (MSCs) and articula

163 Cocultures of Pelobacter SFB93, a C2H2-fermenting bacter

164 The cocultures of platelets with synovial fluid cells from r

165 s question with laboratory microcosms, using cocultures of two bacterial species, P. putida and P. ve

166 Cocultures of ZR75-1 and LNCaP with BMSCs exhibited para

167 end, endothelial cells and fibroblasts were cocultured on 3D scaffolds for 1, 7, or 14 d to form vas

168 When cocultured on exosomes from Nischarin-positive cells, br

169 mental approaches with a synthetic anaerobic coculture pairing fermentative Escherichia coli and phot

170 In one cell-based coculture PD-1 signaling assay, 2a and 2b were 8.2- and

171 While this coculture phenomenon is compelling, the differential cho

172 When we cocultured polarized endocervical cells with HIV-1-infec

173 TAMs were generated by coculturing primary human macrophages (MPhi) with human

174 Cocultures remained viable throughout.

175 T cell stimulation in CD4(+) T cell/monocyte cocultures resulted in maintenance of IL-10-producing T

176 ligands in the three-dimensional organotypic coculture revealed increased mesenchymal expression of W

177 Gene expression analysis after coculture revealed simultaneous induction of PD-L1, IDO1

178 Surprisingly, donor-recipient cocultures revealed that the mere presence of auxotrophi

179 c PTM analysis of colorectal cancer organoid cocultures reveals that shApc, Kras(G12D) and Trp53(R172

180 Confirmatory ex vivo coculture studies validated that SLAMF7-SLAMF7 interacti

181 postimplantation stages by using an in vitro coculture system and profiled the transcriptome of 476 i

182 developed an engineered 3D fibroblast tumor coculture system and used high resolution images to quan

183 oietic stem and progenitor cell/EC (HSPC/EC) coculture system as well as in vivo EC infusions followi

184 dies using an in vitro CD11b(+) cell:Th cell coculture system revealed that CD11b(+)CD11c(+) dendriti

185 etrial epithelial cells (EEC) in an in vitro coculture system revealed transcriptional repression of

186 epithelial polarity loss also occurs in a 3D coculture system that combines acini with human mammary

187 We experimented on an in vitro coculture system to determine its direct effects on the

188 This coculture system will be useful to understand the comple

189 stablished an in vitro fibroblast-epithelial coculture system with primary kidney fibroblasts from RS

190 In a coculture system, adipocytes secreted the cytokines IL-6

191 Using a coculture system, we determined that senescent cholangio

192 anced their cytotoxic activity in a lymphoma coculture system.

193 ation of human hepatocytes was measured in a coculture system.

194 In human coculture systems, we demonstrated that the agonism of t

195 n oligodendrocyte precursor cells (OPCs) and coculture systems, we find that BZA enhances differentia

196 Using motoneuron-CD8(+) T cell coculture systems, we found that mutant SOD1-expressing

197 n response to MCC cells with restored STING, cocultured T cells expressing MCPyV-specific T cell rece

198 egulation of PD-L1 levels, which inactivates cocultured T cells in vitro, compromises anti-tumor immu

199 T(H)2 bias and inducing IL-10 production in cocultured T(H) cells.

200 ro using the Human oxygen-Bacteria anaerobic coculture technique.

201 This coculture technology can have broad implications for use

202 es of amino acid biosynthesis were higher in coculture than in axenic culture, and this was reflected

203 al organoids; and fibroblast-epithelial cell coculture, the latter coupled with fluorescence-activate

204 ntial chondroinductivity of zonal CHs on MSC cocultures, the nature of the molecular cargo, and their

205 Coculturing these two strains with Cd(II) led to the pro

206 The inability to coculture trophozoites and epithelial cells under optima

207 ophils isolated from mouse brain tumors kill cocultured tumor cells.

208 s were used: A three-dimensional organotypic coculture using primary human lung cells, precision-cut

209 e monocultures and oligodendrocyte-astrocyte cocultures, we demonstrate blocking PAR1 improves myelin

210 Cells grown either independently or coculture were exposed to OS inducing cigarette smoke ex

211 cells (BMDCs), and moDC/naive CD4(+) T-cell cocultures were analyzed by using ELISA and flow cytomet

212 In addition, neuron-microglia cocultures were exposed to oxyhemoglobin to mimic SAH in

213 Human and mouse neutrophil-epithelial cocultures were used to evaluate the role of neutrophil-

214 both Krox20 and MBP in SC-motor neuron (MN) coculture, which was notably prevented by pharmacologica

215 riod of experimental evolution, this time in coculture with a B(12)-producing bacterium.

216 lls, among other cancer cells, are CTLA-4(+) Coculture with activated human T cells induced surface C

217 Coculture with adipose stromal cells enabled the metabol

218 Here, we demonstrate that juvenile CHs in coculture with adult MSCs promote functional differentia

219 ability of Legionella pneumophila to grow in coculture with amoebae.

220 upregulated in WAT-derived progenitors after coculture with breast cancer: granulocyte macrophage col

221 or in vivo but attenuated atrophy induced by coculture with cancer cells in vitro.

222 d activity of 5-LO in TAMs were reduced upon coculture with cancer cells.

223 reased, initiation of infection in trans via coculture with CD169(+) IFN-alpha-treated DCs restored i

224 nd reducing expression of Th2 cytokines upon coculture with CD4(+) T cells.

225 In turn, the activation of NK cells in coculture with CMV-specific CD8 T cells promoted a selec

226 After coculture with coinfected DCs, M. tuberculosis Ag-specif

227 , defined by their proliferative response on coculture with dendritic cells from Ang (angiotensin) II

228 cells expressing EPHB1 form aggregates upon coculture with ephrin B1 expressing cells.

229 Furthermore, coculture with gammadelta T cells results in heightened

230 Coculture with human peripheral blood mononuclear cells

231 wn axenically with B12 supplementation or in coculture with M. loti.

232 d NK cells that produced less IFN-gamma upon coculture with M2.

233 g a physiologically realistic microvessel in coculture with mammary tumor organoids.

234 imary airway epithelial cells in organotypic coculture with mast cells, we show that epithelial-deriv

235 ls grown in pure culture on crotonate and in coculture with Methanospirillum hungatei on crotonate, b

236 These genes were switched on by coculture with osteoblastic cells.

237 iation of hPSCs, our system does not require coculture with other cell types and relies on chemically

238 e compactly clustered tumor-cell colonies in coculture with PC3 cells, which might boost tumor stem-l

239 s ERV virions could not be amplified through coculture with permissive cells.

240 Direct coculture with postnatal cFbs increased cardiomyocyte bi

241 rapid activation in response to S. aureus In coculture with S. aureus-infected monocyte-derived dendr

242 s in the pathology, we performed muscle cell coculture with the Abs.

243 G. vaginalis exhibited more-rapid growth in coculture with the tissue model when it was exposed to t

244 species from the human oral cavity in binary coculture with their bacterial hosts.

245 Having isolates growing in coculture with their hosts allowed time course studies o

246 g an infectious clinical reovirus isolate in coculture with three FLA, namely, Vermamoeba vermiformis

247 r of lipid responses in ovarian cancer cells cocultured with adipocytes.

248 lts revealed that at both temperatures, NTHI cocultured with airway epithelial cells demonstrated sig

249 achieved in CA IX overexpressed cells, when cocultured with ALP overexpressed cells, where the nanoa

250 ATC CM-primed neutrophils were cocultured with ATC cells to determine the effects exert

251 nonstimulated CD8(+) cells were purified and cocultured with autologous monocytes infected with adeno

252 They were cocultured with B cells to induce their terminal differe

253 by comparing caries lesions on enamel blocks cocultured with biofilms treated with sucrose, glucose a

254 e was confirmed in patient-derived organoids cocultured with BMSCs.

255 When cocultured with breast cancer cells in vitro, MCs hinder

256 nditioned media (CM) from endocervical cells cocultured with BVAB (endocervical+BVAB CM), as well as

257 ession and IL-12 secretion compared with DCs cocultured with CD4(+) conventional T cells from CD83(fl

258 ylation and cell growth in malignant B cells cocultured with CD40L-expressing stromal cells.

259 Strikingly, DCs from BALB/c mice cocultured with CD83-deficient CD4(+) conventional T cel

260 When enteroids are cocultured with CD90(+) mesenteric lymph node cells from

261 ealthy donors, incubated with interleukin 5, cocultured with fibroblasts, and analyzed by immunohisto

262 9a cells) and mouse enteroid monolayers were cocultured with human macrophages (THP-1, U937, primary

263 T cells can be therapeutically beneficial if cocultured with IL-12 cytokine during in vitro expansion

264 Here, we showed that MCV(+) MCC cells cocultured with keratinocytes undergo neuron-like differ

265 TH17 cells were also cocultured with lung APC subsets to determine which of t

266 ulation and cytotoxic activity than NK cells cocultured with M1.

267 Also, CD56(dim) NK cells cocultured with M2 displayed lower degranulation and cyt

268 from the model were satisfied when Fibs were cocultured with MCs and fluorescence exchange was relate

269 Furthermore, in macrophages cocultured with MDA-MB-231 cells expressing miR-149, epi

270 esting a direct neurotrophic effect, B cells cocultured with mixed cortical cells protected neurons a

271 NPIs cocultured with MSCs had greater cellular insulin conten

272 To assess T-cell differentiation, mDCs were cocultured with naive T(H) cells.

273 Failing hMSCs or nonfailing hMSCs were cocultured with normal human cardiac myocytes derived fr

274 MCF7-derived ducts cocultured with obese stromal cells exhibited higher max

275 enerated from WT or Nod1(-/-) mice that were cocultured with or without WT or Nod1(-/-) macrophages.

276 Las-bearing psyllid insect vectors, and CLas cocultured with other bacteria but at CLas titers below

277 Naive T cells were cocultured with pDCs in specific strain combinations and

278 Naive T(H) cells cocultured with PN-stimulated mDCs showed an RA-dependen

279 omic and proteomic profiling of cancer cells cocultured with primary human omental adipocytes.

280 th colon and lung CAF secreted netrin-1 when cocultured with respective cancer cells, and netrin-1 up

281 ravel molecular pathways of C. albicans when cocultured with S. mutans in mixed biofilms.

282 (SEA)-nonreactive naive CD4 Tcon cells were cocultured with SEA-reactive allergen-nonspecific Treg o

283 lt, cytotoxic NK cells become activated when cocultured with sorafenib-treated MPhi, leading to tumor

284 Giardia chronically cocultured with specific cell lines became adapted (cond

285 Medicago truncatula plants were cocultured with the AM fungus Rhizophagus irregularis un

286 However, macrophages cocultured with the clinical isolate of Acanthamoeba pro

287 sive capacity of myeloid cells was tested in cocultures with autologous lymphocytes.

288 moted IL-4 secretion and suppressed IL-17 in cocultures with autologous T cells.

289 peatedly adapted to grow stably in long-term cocultures with Caco2, Cos7, and mouse tumor rectal (RIT

290 in synthetic biology to construct synthetic cocultures with desired behavior.

291 g reduced TEER and increased permeability in cocultures with human IEC or mouse enteroid monolayers,

292 vivo Treatment of dendritic cell (DC)-T cell cocultures with IFN-alpha upregulated CD169 expression o

293 High levels of TNF were detected in cocultures with nonclassical/intermediate monocytes, the

294 In cocultures with primary human cancer cells, actively mig

295 of primary human airway epithelial cells and cocultures with Pseudomonas aeruginosa.

296 The electrode also allows coculturing with Shewanella for syntrophic electrogenesi

297 in GPC-1 knockdown PC-3 cells was rescued by coculturing with stromal cells.

298 CD147 in HGFs were enhanced after transwell coculturing with U937 cells and exposure to U937-conditi

299 e the release of soluble CD147 in HGFs after coculturing with U937 cells and its functional effect on

300 from astrocytes by evaluating the effects of coculturing WT neurons and BDNF-deficient astrocytes.