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1 ects which could be restored through genetic complementation.
2 separated genetically and undergo intragenic complementation.
3 exogenous WT MELK, but not kinase-dead MELK complementation.
4 an chimerism can be generated via blastocyst complementation.
5 the likely structural basis of interallelic complementation.
6 cture indicate that local spread facilitates complementation.
7 C and following ZPN subdomains by beta-sheet complementation.
8 nomes and their products and thereby reduces complementation.
9 A3B catalytic activity and reversible by UNG complementation.
10 genome-wide association studies and allelic complementation.
11 hetic intermediates and function in chemical complementation.
12 ed adhesion could be restored by chromosomal complementation.
13 rmatic pipeline, and confirmed by transgenic complementation.
14 enotype was reversed by genetic and chemical complementation.
15 g single-EPS-cell-derived mice by tetraploid complementation.
16 was detected using bimolecular fluorescence complementation.
17 s in tt4 are reversed by genetic or chemical complementation.
18 ce microscopy using Bimolecular Fluorescence Complementation.
19 sis, and in one case confirmed by transgenic complementation.
20 n Drosophila was completely amenable to PNKP complementation.
21 evel of plaque formation without X174 J gene complementation.
22 confirmations with bimolecular fluorescence complementation.
23 pability of VTL4 and VTL8 was shown by yeast complementation.
24 with bioactive macromolecules via split GFP complementation.
25 control of the TPS1 promoter, and tested for complementation.
26 f incomplete viral genomes showed that their complementation accounts for the moderate reliance on mu
29 lopment of an aptamer-initiated fluorescence complementation (AiFC) method for RNA imaging by enginee
32 mplex structure and bimolecular fluorescence complementation analyses revealed that the C terminus of
34 double-ring mHsp60 variants are active, and complementation analysis in bacteria shows the single-ri
37 g yeast-two-hybrid, bimolecular fluorescence complementation and co-immunoprecipitation assays, we id
39 nse variants, through a combination of yeast complementation and enzymatic assays, show impaired enzy
40 ation of electrophysiology, yeast functional complementation and extended molecular dynamics simulati
42 as well as improved efficiency in tetraploid complementation and human-mouse interspecies chimerism a
47 SUP) gene based on gene expression analysis, complementation and overexpression assays, and reverse g
49 mentary approaches (bimolecular fluorescence complementation and reverse genetics) demonstrated that
50 t development using a combination of genetic complementation and stacked mutants, biochemical activit
54 ese residues in the RRS1-R allele by genetic complementation, and found that C15 in the Toll/interleu
55 ugh fine mapping, genome sequencing, genetic complementation, and gene editing, that haploid inductio
57 rom the rDNA was not adequate for functional complementation, and the stalled cells arrested prior to
63 of-of-principle for interspecific blastocyst complementation as a viable approach for kidney generati
65 analyses, a mouse embryonic stem cell (mESC) complementation assay and retrieval of patient-related i
68 rmed validation screening with a Split-Rluc8 complementation assay that identified reticulon 1A (RTN1
69 Therefore, we optimized a firefly luciferase complementation assay to screen against this chimeric re
70 bining biochemical approaches with a genetic complementation assay using Arabidopsis thaliana plants,
72 Using a cell-based bimolecular fluorescence complementation assay, here we demonstrate that Nef recr
81 , we used the BiFC (bimolecular fluorescence complementation) assay and report for the first time on
87 establish, using biochemical approaches and complementation assays in live cells, that Dictyostelium
88 derived neurons and bimolecular fluorescence complementation assays in transfected cells to elucidate
91 sis and craniofacial patterning, and in vivo complementation assays indicate that the patient-derived
99 cAMP measurements, bimolecular fluorescence complementation assays, and CD-based helicity assessment
100 ll-down and in vivo bimolecular fluorescence complementation assays, CIPK23 was not phosphorylated by
101 za A virus polymerase using split luciferase complementation assays, coimmunoprecipitation, and in si
104 Bs and 3B fusion products in competition and complementation assays, we investigated the contribution
105 east two-hybrid and bimolecular fluorescence complementation assays, we probed recombinant flax trans
111 erse natural DWV population, suggesting that complementation between genotypes was not required to re
112 we demonstrated by interspecific blastocyst complementation between mouse and rats, generation of pl
115 through the use of bimolecular fluorescence complementation (BiFC) and super-resolution microscopy.
117 noprecipitation and bimolecular fluorescence complementation (BiFC) assays were performed to validate
119 erved in vivo Using bimolecular fluorescence complementation (BiFC) methods, we have now analyzed the
120 Yeast-2-hybrid and bimolecular fluorescence complementation (BIFC) results showed that the transmemb
123 ngs in mice through a conditional blastocyst complementation (CBC) approach that vacates a specific n
124 rotein engineering tools, including fragment complementation, circular permutation, and domain swappi
126 pollen exine development, and by transgenic complementation demonstrate that TaMs5-A restores fertil
127 east two-hybrid and bimolecular fluorescence complementation) demonstrate that StCEN binds components
128 employed, while the reciprocal heterologous complementation demonstrates that U. botrytis MAT genes
129 y, these genes were capable of cross-species complementation, demonstrating that trans-acting factors
132 structural studies, we present a functional complementation experiment in E. coli AR3110, indicating
133 These results alongside a range of genetic complementation experiments are discussed in the context
136 with inner-envelope proteins, and functional complementation experiments in a Columbia-0 ftsZ2-2 null
146 his pathway by mutation of genes encoding FA complementation group (FANC) proteins impairs developmen
148 DNA-bound, monoubiquitinated Fanconi anemia complementation group D2 (FANCD2) protein in the Fanconi
150 m this interaction in cells deficient for FA complementation group I and D2 (FANCI and FANCD2) that f
151 notype-phenotype correlation within the FA-B complementation group of FA, where severity is associate
154 Interspecies organ generation via blastocyst complementation has succeeded in rodents, but not yet in
162 hrough targeted gene deletion and functional complementation in Malassezia sympodialis, we demonstrat
165 have characterized the role of GmVTL1a using complementation in plant mutants, hairy root transformat
168 es is an extreme instance of gene expression complementation, in which genes are active in only one o
169 rod through a mechanism termed donor strand complementation, in which the incomplete immunoglobulin-
170 ilus rod via a mechanism termed donor-strand complementation, in which the N-terminal extension of ea
172 mologs in diverse bacterial phyla, and cross-complementation indicates that MrfAB function is conserv
173 ysed mutants deficient for both proteins and complementation lines (with the AXR1 promoter and either
175 yte defects were bypassed by specific pollen complementation, loss of MED30 led to early embryo devel
185 logical functions of mitochondria, including complementation of damaged mitochondrial DNAs and the ma
186 In addition, metabolite analysis and genetic complementation of glycolate transport in yeast showed t
189 ng viable mutant plants generated by partial complementation of homozygous lethal OXA2a transfer-DNA
191 in of murine astrovirus in the gut, and this complementation of immunodeficiency required IFN-lambda
193 presence of EHB1 prevented the IRT1-mediated complementation of iron-deficient fet3fet4 yeast (Saccha
198 tch into the ONE HELIX PROTEIN1 gene allowed complementation of ohp1 mutants and induction of the mut
202 studies have demonstrated that interspecies complementation of rodent blastocysts lacking a developm
203 tified in bioinformatics studies and in vivo complementation of select mutants; however, none of thes
205 d in density gradients and allowed the trans-complementation of subgenomic reporter HEV replicons.
210 iate complexes containing cytochrome b (559) Complementation of the Chlamydomonas reinhardtii (hereaf
213 GFP-tagged protein variants, and phenotypic complementation of the yeast strain Kl3, we identified r
214 nize bark-covered wood, suggesting potential complementation of their weaker wood-decaying abilities
215 expression of ZAP, indicative of synergy or complementation of these hitherto unconnected pathways.
216 ion phenotype on cotton, olive, and okra but complementation of two genes restored the defoliation ph
218 ability assays, revealing 50 new human-yeast complementation pairs, thus opening up avenues to furthe
219 NAP tags improve upon other protein fragment complementation (PFC) approaches by offering both multid
220 Here, we establish a versatile blastocyst complementation platform based on CRISPR-Cas9-mediated z
221 ficient conjugative transfer, 'extracellular complementation' potentially involving intercellular pro
222 nsional printing and interspecies blastocyst complementation, promise organoids manufactured from the
224 ns in specific genes of interest followed by complementation remains problematic for obligates and is
225 ically relevant, we use a recently developed complementation reporter system to evaluate the kinetics
226 loped NanoLuciferace (NLuc)-based structural complementation reporter system which allows us to obser
229 east two-hybrid and Bimolecular Fluorescence Complementation revealed that while all the AtPam18s int
230 pe analysis, as well as chemical and genetic complementation, revealed that CEK3, a Cho-specific kina
231 discovered rare alleles by zebrafish in vivo complementation reveals an enrichment for pathogenic all
232 or synapsis and recombination, using a novel complementation strategy that exploits a previously unch
233 pproach that applies a cell-surface fragment complementation strategy, Split-TurboID, and identify a
236 east two-hybrid and bimolecular fluorescence complementation studies demonstrated interaction of both
242 ying a forward genetic approach supported by complementation studies of ecotype Columbia (Col), which
243 was demonstrated using reverse genetics and complementation studies of h2ax mutants, establishing th
248 We confirmed these findings in a genetic complementation study that involved crossing FHH and MNS
249 demonstrates the potential of the NS1 trans complementation system as a new platform for flavivirus
250 s have demonstrated the potential of the NS1 complementation system as an alternative platform for fl
253 Here, we report on a nanoluciferase-based complementation technique to detect ligands that promote
255 in live cells that utilizes split-luciferase complementation technology based on TREM2 and TYROBP fus
256 , based on the DiscoverX InCell Pulse enzyme complementation technology, we characterized the binding
259 We validate its effect using a quantitative complementation test with CRISPR/Cas9-generated null mut
267 coupled to a supported lipid bilayer via DNA complementation, triggers TCRs and activates nuclear tra
272 genome editing, transposon mutants, and gene complementation, we empirically assessed the contributio
273 unoprecipitation and bimolecular florescence complementation, we found that SsCP1 interacts with PR1
275 tion microscopy and bimolecular fluorescence complementation, we map protein distribution at SPB fene
276 CRISPR-mediated gene knockout and subsequent complementation, we validate an essential role for the e
277 ach, demarcated by dystrophin exons 2-79, in complementation with a cardiac and skeletal muscle direc
282 nally, rescue of an fst1 insertion mutant by complementation with an FST1 genomic fragment restored t
285 icromolar concentrations of putrescine or by complementation with either glycosomal or cytosolic vers
289 duces bright luminescence upon high-affinity complementation with LgBiT, an 18-kDa subunit derived fr
294 cytoplasmic O-fucosyltransferase (OFT) by 1) complementation with TgSPY-MYC(3), 2) its functional dep