ライフサイエンスコーパス: concatemer

1 through autonomous formation of a signaling concatemer.

2 ne of the two genomic ends are formed on the concatemer.

3 g pathway that is initiated by cleavage of a concatemer.

4 sistent with the integration of het DNA as a concatemer.

5 on is introduced to the gamma2L-beta2-alpha1 concatemer.

6 molecular DNA packaging machine on the viral concatemer.

7 D3 through disulfides to form ultralong VWF concatemers.

8 pports strong FRET in CFP-DsRed or GFP-DsRed concatemers.

9 ystem for replication and maintenance of DNA concatemers.

10 n hepatocytes containing the double-stranded concatemers.

11 mediate cleavage of genomes from replicative concatemers.

12 zation) of T7 DNA and subsequent cleavage of concatemers.

13 cated product is in the form of long plasmid concatemers.

14 lling circle activity to generate the linear concatemers.

15 nome, arranged mainly as polydisperse linear concatemers.

16 to rolling circle amplification to generate concatemers.

17 SBs) totaling 5.9 kb that exist as nonrandom concatemers.

18 ndividual binding steps to each subunit with concatemers.

19 ncatemers; and locus 7 yielded no identified concatemers.

20 ATR in "repair" of E4 mutant genomes to form concatemers.

21 of alpha3beta4alpha5 versus alpha3beta4-only concatemers.

22 plication and ligates the viral genomes into concatemers.

23 NA replication and ligate viral genomes into concatemers.

24 , and joining together of viral genomes into concatemers.

25 nd functional characterization of tetrameric concatemers.

26 lly characterized by the formation of genome concatemers.

27 We propose the use of concatemer, an artificial and stable isotope-labelled pr

28 EGF) expressions were controlled by this HRE concatemer and a minimal simian virus 40 promoter.

29 ultiple genome copies combined into a single concatemer and by analysing reads generated from plus an

30 milar pore properties of the hexameric Orai1 concatemer and native CRAC channels, we conclude that th

31 es are then cut out of a larger, multigenome concatemer and packaged into capsids.

32 echanism, the packaging machinery cuts a DNA concatemer and packages a single unit length genome with

33 osome involves cutting the chromosome from a concatemer and translocating the DNA into a prohead.

34 d that it existed as a high-molecular-weight concatemer and underwent significant levels of homologou

35 me quantification of concentrations of rLTNF concatemer and urea in the reaction mixture.

36 wild-type and mutagenized PSA promoter, ARE concatemers and appropriate controls.

37 We find a head-to-tail arrangement in the concatemers and circular permutation in both the monomer

38 circulates in the blood as disulfide-linked concatemers and functions in primary hemostasis.

39 Monomeric herpesvirus DNA is cleaved from concatemers and inserted into preformed capsids through

40 rpes simplex virus (HSV) DNA is cleaved from concatemers and packaged into capsids in infected cell n

41 enzyme that excises individual genomes from concatemers and packages them into preassembled procapsi

42 stoichiometry, while studies of linked Orai1 concatemers and single-molecule photobleaching suggest t

43 gene expression (including the formation of concatemers and their subsequent cloning and sequencing)

44 sized linear molecules, head-to-tail genomic concatemers, and complex branched forms with ends at def

45 DNA genomes, in the form of complex branched concatemers, and unstable spherical precursor capsids te

46 2, and 5 produced head-to-head/tail-to-tail concatemers; and locus 7 yielded no identified concateme

47 We have used a pentameric concatemer approach to express defined and consistent po

48 rgens (egg, milk, peanut, and hazelnut), the concatemer approach was found to offer advantages associ

49 o form several fragments is observed when T7 concatemers are incubated in an extract of T7-infected E

50 In vivo, DNA concatemers are required for packaging.

51 vage of herpesvirus genomes from replicative concatemers are unknown.

52 9-kb band, suggesting a head-to-tail genomic concatemer as the most prominent form in extracted mtDNA

53 and beta2 subunits and establish pentameric concatemers as a means to delineate interactions between

54 bunits increased the rate of inactivation of concatemers, as predicted for subunits that act independ

55 hesis is proposed: the observed packaging of concatemer-associated T7 genomes is cooperative.

56 The concatemers autonomously performed many functions attrib

57 alpha5, or alpha5DN subunits with a dimeric concatemer (beta2alpha4) in a heterologous system, to ob

58 nown to occur during the packaging of T7 DNA concatemers both in vivo and in vitro.

59 I, are able to produce normal amounts of DNA concatemers but they are not cut, or matured, into unit

60 region, the viral genomic DNA is joined into concatemers by cellular DNA repair factors, and this req

61 lly regardless of which Orai1 subunit in the concatemer carried the mutation.

62 veral technical difficulties associated with concatemer cloning and purification have not been solved

63 d steps, such as purification and cloning of concatemers, colony picking and plasmid DNA purification

64 The packaging substrate is typically a concatemer composed of multiple genomes linked in a head

65 Phage genomes are replicated as linear concatemers composed of multiple copies of the genome jo

66 e large dsDNA viruses replicate their DNA as concatemers consisting of multiple covalently linked gen

67 n range from single copy insertions to large concatemers, consisting of complex DNA originating from

68 tudies utilizing applied transcriptomics and concatemer constructs support that up to one or two KV1.

69 The RCA product is a concatemer containing tens to hundreds of tandem repeats

70 MutY were found to function processively on concatemers containing 7,8-dihydro-8-oxo-2'-deoxyguanosi

71 nstructed and characterized tetrameric hERG1 concatemers containing a variable number of wild-type su

72 on during replication can produce h-t linear concatemers containing an inversion of single copy seque

73 ed initiators triggered the formation of DNA concatemers containing hemin-binding aptamers through a

74 Poxvirus DNA replication generates linear concatemers containing many copies of the viral genome w

75 as not observed in the reaction of MutY with concatemers containing OG.A mispairs.

76 If the in vivo presence of a similar concatemer-containing DNA network is assumed, requiremen

77 ded by Ad3, Ad7, Ad9, and Ad11, no viral DNA concatemers could be detected.

78 en proposed that the phage packages a linear concatemer created during rolling circle replication of

79 The translocation ATPase and the concatemer-cutting endonuclease reside in terminase.

80 Formation of pac2 ends on concatemers depended on terminal cis sequences, since ec

81 SOAR itself is a dimer, we constructed SOAR concatemer-dimers and introduced mutations at F394, whic

82 complex that mediates both the insertion of concatemer DNA into capsids and its subsequent cleavage

83 tein complex that mediates both insertion of concatemer DNA into capsids and its subsequent cleavage

84 oli and poor in vitro ligation of CTG repeat concatemers due to strand slippage.

85 Mature genomes are cleaved from the concatemer during packaging.

86 from transient head-to-head and tail-to-tail concatemers during replication in the cytoplasm of infec

87 n internal phosphodiester bond and linearize concatemers during rolling circle replication.

88 Often, such short ssDNA dominantly form concatemers (either linear or circular) due to intermole

89 stent with a model in which pac2 elements at concatemer ends impart a directionality to concatemer pa

90 on is that single genomes are cleaved off of concatemer ends in a preferred direction.

91 binding proteins that initiate insertion of concatemer ends into empty capsids.

92 molecules to generate a head-to-tail linear concatemer, followed by recombination-dependent replicat

93 icating cleavage/packaging of viral DNA from concatemers for packaging into virions, but analyses of

94 Mre11, Rad50, and Nbs1 (MRN) is required for concatemer formation and full activation of damage signa

95 gated the cellular proteins involved in this concatemer formation and how they are inactivated by E4

96 of Nbs1 that are differentially required for concatemer formation and inhibition of Ad DNA replicatio

97 12 also failed to complement defects in both concatemer formation and late protein production of a vi

98 Concatemer formation can be prevented by the E4orf3 prot

99 Concatemer formation is dependent upon the cellular Mre1

100 plication in the absence of E4 is not due to concatemer formation or DNA damage signaling.

101 individual contributions of the MRN complex, concatemer formation, and damage signaling to viral DNA

102 host nucleus, resulting in circularization, concatemer formation, or chromosomal integration.

103 ation was a critical event in the process of concatemer formation.

104 transcriptional crosstalk is dependent upon concatemer formation.

105 B-55K to degrade MRE11, preventing viral DNA concatemer formation.

106 junctions between genomes within replicative concatemers formed late in infection almost exclusively

107 ytes showed double-stranded and head-to-tail concatemer forms but failed to show integration of the A

108 The results revealed that amplicon concatemers frequently contain adjacent amplicon units w

109 d recombination may result in viral-host DNA concatemers, frequently disrupting genes involved in onc

110 Mouse fibroblasts transfected with the concatemers gave a CAT activity that was 14-fold greater

111 a DNA-barcoded antibodies and orthogonal DNA concatemers generated by primer exchange reaction (PER).

112 1) excision of a unit length genome from the concatemer (genome maturation) and 2) translocation of t

113 n in equilibrium between circular and linear concatemer genomes caused by the lack of DNA-PKcs activi

114 same region of high tension in tethered VWF concatemers; however, the half-maximal tension required

115 CAP-treated cells displayed progressive concatemer immobilization with increasing molecular weig

116 A1 is activated by tensile force on VWF concatemers imparted by hydrodynamic drag force.

117 ons in the reaction buffer, concentration of concatemer in the IBs, enzyme loading relative to protei

118 ed rAAV molecules into high-molecular-weight concatemers in about 5% of hepatocytes.

119 e chromosomal islands, which are packaged as concatemers in phage particles, with lengths that match

120 fluorescent protein fusion peptides (AcGFP1 concatemers) in the mitochondrial matrix of HeLa cells d

121 o formation and accumulation of head-to-tail concatemers, in addition to the usual head-to-head and t

122 the liver was approximately 0.2, suggesting concatemer integration.

123 (6A(4)) or SP-A2(1A(3)) were integrated as a concatemer into the genome of each of the two hTG lines.

124 e copies of the transgene can integrate as a concatemer into the sperm genome, and more than one site

125 ay junction resolvase is required to process concatemers into unit-length genomes for packaging.

126 Processing of viral DNA concatemers into unit-length genomes was unimpaired at e

127 ired for the in vivo resolution of viral DNA concatemers into unit-length genomes with hairpin telome

128 ) is defective in both cleavage of viral DNA concatemers into unit-length monomers and packaging of v

129 The loss of long VWF concatemers is associated with the excessive bleeding of

130 aves the concatemerized DNA within this 8 nt concatemer junction (CJ).

131 near promoters and near the right end of the concatemer junction almost certainly must relate to lyso

132 DNA and at a site near the right end of the concatemer junction of T7 DNA.

133 T/A), well conserved at the right-end of the concatemer junction of T7-like phages.

134 aining the entire VAC genome, with an intact concatemer junction sequence, were identified.

135 ion and in pausing and termination at the T7 concatemer junction.

136 85) reduce pausing and termination at the T7 concatemer junction.

137 eaves DNA four-way junctions extruded at the concatemer junctions to produce monomeric genomes.

138 -field gel electrophoresis, (iv) cleavage of concatemer junctions was inhibited, and (v) virion morph

139 mechanism, we used a series of V. riparia K2 concatemers (K4, K6, K8, and K10) and natural dehydrins

140 whose repeat structures, gene contents, and concatemer lengths suggest they are phage-inducible chro

141 n and therefore were not part of a canonical concatemer made by replication.

142 wed that packaged phage had not been part of concatemers made by recombination or by annealing at cos

143 suggesting that both circular and linear AAV concatemers may have contributed to the trans-splicing-m

144 factor-1alpha during hypoxia and features a concatemer of four XRE cores (GCGTG).

145 A concatemer of nine copies of the consensus sequence of H

146 Integration into a 32-unit concatemer of target DNA was markedly more efficient tha

147 This is the first time that a concatemer of the complete pentameric receptor has been

148 A concatemer of the cpr-1 -147 GATA motif placed upstream

149 nant allele, V99A(T)/V99A(T), a head-to-tail concatemer of three V99A targeting constructs.

150 Concatemers of d(TCCC) that were first detected through

151 ents lie near the ends formed on replicative concatemers of four herpesviruses: herpes simplex virus

152 Concatemers of high-order contacts in highly expressed g

153 rain stoichiometry, fusion proteins encoding concatemers of human alpha3, beta4, and alpha5 (D and N

154 hannel stoichiometry by expressing hexameric concatemers of human Orai1 and comparing in detail their

155 ese models, we have designed and synthesized concatemers of Rev-binding elements (RBEs) that fold to

156 hat the presence of two ER export motifs (in concatemers of SERT and GABA transporter-1) supported re

157 By using concatemers of subunits and chimeric subunits, we have f

158 We generated concatemers of subunits so that the alpha1 subunits coul

159 ce found at the junction of the head-to-tail concatemers of T7 genomic DNA generated during T7 DNA re

160 ealing (SSA) reaction to generate end-to-end concatemers of the phage genome for packaging.

161 n defined by the primers but also continuous concatemers of the template.

162 adenovirus E4 mutants accumulate end-to-end concatemers of the viral genome that are assembled from

163 Multiple copy transformants often integrated concatemers of transforming DNA.

164 was based on circularization of head-to-tail concatemers of VAC DNA.

165 te for packaging is a head-to-tail multimer (concatemer) of the mature 40-kilobase pair genome.

166 A CanScript concatemer offered enhanced activity.

167 Human RK (hRK) promoter and its concatemers or derivatives extending into the conserved

168 ment formed at the junction of HHV-6B genome concatemers (pac2-pac1) is necessary and sufficient for

169 t concatemer ends impart a directionality to concatemer packaging by binding proteins that initiate i

170 mical evidence of a requirement for gp55 for concatemer packaging to assemble active wild-type phage

171 Integrations involving amplified HPV-human concatemers, particularly multi-breakpoint events, frequ

172 ation intermediates the loci produced, while concatemer processing gave rise to the circularized DNAs

173 xynucleoside triphosphates and a polymerase, concatemers quickly formed, and those random sequences t

174 donucleolytic cleavage of immature viral DNA concatemer recognized by TerS, assembles into a pentamer

175 us genome mediated formation of pac2 ends on concatemers regardless of the orientation of their inser

176 ion and maintenance in Escherichia coli; DNA concatemer resolution was inhibited leading to formation

177 and roles in DNA replication, recombination, concatemer resolution, and transcription were suggested.

178 Some plasmids exploit the Xer machinery for concatemer resolution.

179 ry high frequency: up to 60% of the amplicon concatemers retrieved from virion particles underwent in

180 nisms in the same or alternate subunits of a concatemer revealed that both intra- and intersubunit co

181 The observed in vitro packaging of a concatemer's genomes always occurs in a synchronized clu

182 Our data suggest that this XRE concatemer site concurrently regulates the expression of

183 ADsubTRS1 exhibited normal cleavage of DNA concatemers, so the defect in C-capsid production must o

184 n them had randomly combined to form complex concatemers, some of which were multi-mega base pairs in

185 the proteomic field for more than a decade, concatemer strategy has not yet been applied for food an

186 s with Y- or X-structures in the replicative concatemer substrate by employing a portal-bound Hollida

187 e used a linear multi-abasic site substrate (concatemer), synthesized by ligating together identical

188 ontinuing around the other end to generate a concatemer that is subsequently resolved into unit genom

189 ndependent in sequence, from the complicated concatemer that LAMP processes create as part of their a

190 Herpesvirus genomic DNA is cleaved from concatemers that accumulate in infected cell nuclei.

191 However, whereas DNA concatemers that accumulate in prohead and terminase def

192 d FISH probes with long, single-stranded DNA concatemers that aggregate a multitude of short compleme

193 The latter generates concatemers that are cleaved and packaged into infectiou

194 s on a massive scale via the medium of cfChP concatemers that have undergone extensive and complex mo

195 es replicate their linear genomes by forming concatemers that must be resolved into monomeric units t

196 Hetero-concatemers that resulted from recombination between min

197 minase introduces staggered nicks to cut the concatemer to generate unit-length virion chromosomes.

198 nylated proteins indicated that breakdown of concatemers to individual subunits was minimal.

199 ormation capture with nanopore sequencing of concatemers to profile proximal high-order chromatin con

200 nded DNA genome of adenovirus is joined into concatemers too large to be packaged.

201 implex virus (HSV) replicates by forming DNA concatemers using a single strand annealing mechanism me

202 Using the multi-abasic site concatemer, we demonstrated that AP endo was capable of

203 ual head-to-head and tail-to-tail forms; the concatemers were circularized by homologous or Cre-loxP-

204 Concatemers were expressed in Xenopus laevis oocytes, an

205 These unusual concatemers were generated through homologous recombinat

206 These concatemers were identical to signaling microclusters, a

207 ate gene expression and resolution of genome concatemers were not detected.

208 Fourth, concatemers were partially digested with NlaIII before c

209 mined that the 25-mer monomer from which the concatemers were prepared was nicked by AP endo in a fas

210 A new model is proposed in which concatemers were separated into single units by a "snap-

211 umulate high-molecular-weight linear plasmid concatemers when transformed with plasmids carrying the

212 ges and herpes viruses replicate genome as a concatemer which is cut by a 'headful' nuclease upon com

213 tranded DNA genomes as high-molecular-weight concatemers which are subsequently cleaved into unit-len

214 y ligation of the digestion products yielded concatemers which migrated as a single band in agarose g

215 the host genome, forming mainly head-to-tail concatemers with occasional deletions of the inverted te

216 hage T4-like networks of highly branched mp1 concatemers with up to 20 monomer units were mapped and

217 near genomic monomers and head-to-tail (h-t) concatemers within inverted repeat sequences (IRs) near

218 e event begins with a single repeat within a concatemer yet produces two repeats, one at each of the

219 is that RCR should generate tandem Helitron concatemers, yet almost all Helitrons identified to date