ライフサイエンスコーパス: conformational

1 substrate binding of OGG1--both resulting in conformational adjustment in the adjacent DNA sequences

2 Conformational analyses of N-acyl isochalcogenouronium s

3 Here, we describe the synthesis and conformational analysis of three delta-azaproline deriva

4 L-MS) provides unique information on protein conformational and interaction changes resulting from pe

5 Patients' IgE recognized conformational and linear epitopes in a patient-specific

6 By removing conformational and linear IgE epitopes, a hypoallergenic

7 ught to determine the relative importance of conformational and linear IgE-binding epitopes of the ma

8 ffects of these ligands on three-dimensional conformational and local changes in BLG induced by HP.

9 h degree of clonal diversity, recognize five conformational antigenic sites of the genotype 1 HEV p23

10 demonstrate that ClpXP degradation modifies conformational assemblies of MinD in vitro and depresses

11 he molecular level, observing changes in the conformational behaviour of monomers, with a possible li

12 Much attention is being paid to conformational biases in the ensembles of intrinsically

13 ever, it is currently unknown whether or how conformational biases within the disordered ensembles of

14 Chromosome conformational capture techniques such as Hi-C, have bee

15 d that the HR3 modulates triggering of the F conformational cascade (still requiring G).

16 One limitation has been that global conformational change - which occurs in all conventional

17 PS), binds NLRP6 directly and induces global conformational change and dimerization.

18 toinhibition, allowing it to undergo a large conformational change and interact itself with the membr

19 Upon binding dsRNA, OAS undergoes a conformational change and is activated to polymerize ATP

20 wed that ATP-DnaA, not ADP-DnaA, undergoes a conformational change at the higher nucleotide concentra

21 A key element of the model is a conformational change from R (relaxed), which is highly

22 The first conformational change in our dataset involves rotation o

23 the isoalloxazine ring of FMN and induced a conformational change in residues of the FMN-binding poc

24 ow acylation by the K234R enzyme is due to a conformational change in Ser(130), and this change also

25 r longer chain fatty acids is regulated by a conformational change in the C-terminal tail that leads

26 echanism is that the mutation predisposes to conformational change in the expressed protein, leading

27 Conformational change in the receptor regulated tightly

28 DEER revealed an agonist-dependent conformational change in which residues of the B'-helix

29 ier to partial dehydration in the absence of conformational change indicates Kir channels are not gat

30 of the bovine and human BBSome reveal that a conformational change is required to recruit the complex

31 The mAb h5B3.1 blocks the required F conformational change needed to facilitate membrane fusi

32 rai1 pore opening is accompanied by a global conformational change of all Orai transmembrane domain (

33 nhibition to an active, open state, due to a conformational change of the ELMO1 subunit, exposes bind

34 le of the E-DNA sensor is the target induced conformational change of the surface signaling probe (co

35 for substrate binding and a fidelity-related conformational change referred to as "fingers closing."

36 Although the cause of this conformational change remains elusive, ADAMTS13 is prima

37 We prove that a local conformational change spreads omnidirectionally within t

38 ding of gD to one of its receptors induces a conformational change that allows gD to bind to the regu

39 Moreover, binding to loxP DNA induces a conformational change that dislodges the C terminus, res

40 thosteric ligand-binding pocket enabled by a conformational change that doubles the pocket volume.

41 indicate that acetylation of Pif1 induces a conformational change that may account for its altered e

42 ral modeling predicts that SY242CS confers a conformational change that mediates stable binding to a

43 T6 but absent from other isoforms, induced a conformational change that resulted in a much stronger a

44 slow unimolecular reaction consistent with a conformational change that stabilized the complex.

45 Of note, this conformational change was not universally fulfilled by a

46 ADPH binding and mutation of Lys166 causes a conformational change, altering protein structure.

47 L) of the serpin, and the serpin undergoes a conformational change, incorporating the amino-terminal

48 ites and coupling between ligand binding and conformational change.

49 virus surface spike, allowing it to undergo conformational changes and become prone to proteolytic a

50 that the ACB domain facilitates induced-fit conformational changes and confers high-affinity RNA hai

51 we demonstrate a direct relationship between conformational changes and enzyme activity that is explo

52 To identify the primary steps of these conformational changes and how they are prevented by the

53 sensitive and robust method to probe protein conformational changes and protein-ligand interactions.

54 t undergoes adenosine triphosphate-dependent conformational changes and recruits MutLalpha.

55 s that Aurora B S227 phosphorylation induces conformational changes and this manifests in a profound

56 solution provides precise information about conformational changes and variations of the interaction

57 boundary indicate that cofilin-induced actin conformational changes are local and limited to subunits

58 egulatory hub in Sth1, suggesting that large conformational changes are part of Sth1 regulation and R

59 Finally, we study the conformational changes associated with the switching of

60 ies of apo and holo states demonstrate local conformational changes at specific sites within the loop

61 between fluorophore motions and large-scale conformational changes between LIV-BPSS domains.

62 These conformational changes control the path of DNA flanking

63 We find that while the conformational changes do not significantly affect elect

64 We hypothesize that the conformational changes due to SCoA binding impact the Cd

65 ABC transporters undergo substantial conformational changes during substrate translocation.

66 esis but clashes with longer RNA-DNA trigger conformational changes for the timely release of the pro

67 Our investigations reveal that conformational changes in EF-Tu coordinate the rate-limi

68 H(+) driven by the proton motive force, and conformational changes in FliG2 driven by CheY3-P allow

69 a2+, and NPC1 binding synergistically induce conformational changes in GP2 and permit virus-liposome

70 based biosensors that detect WNT-induced FZD conformational changes in living cells in order to asses

71 (EPR) has become an important tool to probe conformational changes in nucleic acids.

72 The early receptor potential is caused by conformational changes in opsins after photoisomerizatio

73 These findings shed light on allosteric conformational changes in PCSK9 required for high-affini

74 ninvasive, label-free approach for assessing conformational changes in receptors in living cells at a

75 ows that inhibitor binding is accompanied by conformational changes in the active site, including the

76 ceptors, CD4 and CCR5/CXCR4, triggers serial conformational changes in the envelope glycoprotein (Env

77 ates rotation of the central stalk, inducing conformational changes in the F(1) motor that catalyzes

78 ctivates fusion by facilitating irreversible conformational changes in the glycoprotein.

79 DAGH sheet) are particularly susceptible to conformational changes in the monomeric state.

80 ns helped to propose a plausible sequence of conformational changes in the mu-OR and G(i) system and

81 The former invokes termination via conformational changes in the transcription complex and

82 g correlates cytoskeletal filament size with conformational changes inferred from molecular dynamics

83 folding and unfolding and determining hidden conformational changes invisible to other methods.

84 rotein-based mechanisms of inheritance whose conformational changes lead to the acquisition of divers

85 relaxation dispersion measurements report on conformational changes occurring on the mus-ms timescale

86 These findings suggest that conformational changes of actin filaments in cells could

87 ecule fluorescence spectroscopy to track the conformational changes of bovine MRP1 (bMRP1) in real ti

88 f the l- and d-enantiomers induced different conformational changes of FliY, where the l- enantiomer-

89 or channel and the substrate channel via the conformational changes of its two catalytic residues Cys

90 Using FRET assays to monitor conformational changes of pol beta with r8-oxo-GTP, we d

91 CIU detects subtle conformational changes of proteins and emerges as an att

92 Alternatively, Urd may block conformational changes of the holoenzyme required for en

93 Conformational changes of the photosynthetic antenna com

94 e adenosine triphosphate (ATP) hydrolysis to conformational changes of their catalytic core.

95 demonstration of a small molecule affecting conformational changes on a HS oligosaccharide.

96 escale have been structurally characterized, conformational changes on slower timescales remain elusi

97 g multiple cycles of LIV-BPSS clamshell-like conformational changes on the time scale of seconds, exa

98 We also examine how proton-induced conformational changes pose unique challenges to classic

99 preventing access to the target RNA and the conformational changes required for nuclease activation.

100 Transition paths of macromolecular conformational changes such as protein folding are predi

101 haracterization of the temperature-dependent conformational changes suggests the contribution of the

102 EDD8-dependent interprotein interactions and conformational changes synergistically configure a catal

103 During amyloidogenesis, proteins undergo conformational changes that allow them to aggregate and

104 ophage T7 undergoes large, substrate-induced conformational changes that are thought to account for h

105 bond, but during catalysis thermally induced conformational changes that enable ET from a cluster iro

106 ests receptor binding initiates a cascade of conformational changes that produces expanded particles

107 asugars reveal mechanistic details hidden by conformational changes that the Michaelis complex of the

108 ut signals are converted to the same output: conformational changes that trigger refolding of trimeri

109 c, likely driven by a wave of interdependent conformational changes throughout the protein originatin

110 have focused on residue mobility and global conformational changes to address receptor function.

111 Techniques for capturing the continuous conformational changes underlying function are essential

112 Conformational changes upon ligand binding illuminate a

113 able to proteins undergoing relatively small conformational changes upon target binding.

114 tion through SecYEG and (2) communication of conformational changes via SecDF across the periplasm to

115 These conformational changes were consistent with transfer of

116 forward and reverse directions to correlate conformational changes with chemical reaction steps.

117 nd viscoelastic properties due to reversible conformational changes with mucin forming a network stru

118 ctor-triggered and phosphorylation-regulated conformational changes within RRS1 that results in disti

119 analyze three diverse biological processes: conformational changes within the soluble protein calmod

120 cruit multiple SNARE proteins, trigger their conformational changes, and facilitate SNARE assembly.

121 le ATP binding and hydrolysis to large-scale conformational changes, ultimately leading to substrate

122 igands, suggesting a sequential mechanism of conformational changes.

123 rate TPP nor protonation induces large-scale conformational changes.

124 to characterize the global and peptide-level conformational changes.

125 without the ad hoc postulation of additional conformational channel states.

126 A growing body of literature suggests that conformational characteristics of tau filaments, along w

127 t glycosyltransferase enables testing of the conformational control model.

128 e studies reveal the previously unrecognized conformational cycle of ABCG2.

129 bbons orient similarly in solution; as such, conformational differences do not account for the dispar

130 204-scFv monomer, dimer, and trimer revealed conformational differences that explain differences amon

131 e photon energy after photoisomerization: 1) conformational distortion of the prosthetic group retina

132 ng regions (CDRs) that increase binding site conformational diversity.

133 Tapasin conformational dynamics are also affected by calreticuli

134 rrelate the roles of protein interactions in conformational dynamics at the multidomain level.

135 in the literature of targeted engineering of conformational dynamics being successfully used to alter

136 ze, suggesting coevolution of AimB with MreB conformational dynamics in C. crescentus.

137 Harnessing conformational dynamics in engineering studies is a powe

138 We address the role of enzyme conformational dynamics in specificity for a high-fideli

139 eir calcium binding properties, and in their conformational dynamics in the presence and absence of C

140 This structural arrangement and conformational dynamics may facilitate rapid intraflavin

141 ular dynamics simulations, we confirmed that conformational dynamics of beta2-microglobulin native st

142 c (MD) simulations suggest a large degree of conformational dynamics of ECD that is necessary for bin

143 Allostery exploits the conformational dynamics of enzymes by triggering a shift

144 ability of HDX-MS to distinguish between the conformational dynamics of inhibitor and substrate bindi

145 their spin-labeled variants as reporters of conformational dynamics of membrane proteins using DEER

146 ET) spectroscopy for probing sub-millisecond conformational dynamics of protein folding and binding o

147 ane reconstitution system to investigate the conformational dynamics of SNARE/Munc18-1 complexes in m

148 e and active states, but poorly describe the conformational dynamics of the allosteric network that u

149 ar dynamics simulation results indicate high conformational dynamics of the cytosolic region that reg

150 ations of cone GAFab revealed differences in conformational dynamics of the two subunits forming the

151 f wild-type or S34F-substituted U2AF1 on the conformational dynamics of U2AF2 and its splice site RNA

152 se studies have suggested a role for protein conformational dynamics that has not been well character

153 ght into rate-limiting steps of G-quadruplex conformational dynamics.

154 Here, we uncover a range of conformational effects in full-length BTK induced by a p

155 Subtle conformational effects seen for singly charged metals di

156 ing sequence patterns that favor an expanded conformational ensemble are under selection for efficien

157 interferometry (XSI), to directly probe the conformational ensemble of the Holliday junction across

158 nder specific ionic conditions, the complete conformational ensemble of this motif, especially at low

159 reliable measurement of such distances for a conformational ensemble remains a significant challenge.

160 oid insensitive 1-associated kinase 1 (BAK1) conformational ensemble, we performed Gaussian accelerat

161 es of IDPs is of great interest, since their conformational ensembles are the link between their sequ

162 lthough challenging, characterization of the conformational ensembles of IDPs is of great interest, s

163 0 macrocycles resulted in occupancy-weighted conformational ensembles that exhibited low strain energ

164 tion relationships: two-state and multistate conformational ensembles, conformational hierarchies, os

165 ical pH and temperature to populate relevant conformational ensembles.

166 tegrative structural biology to characterize conformational ensembles.

167 signatures of heterogeneity in the monomeric conformational entropies.

168 pari-Szabo based formalism for estimation of conformational entropy (S(conf)), we find clear signatur

169 Conformational entropy can be an important element of th

170 th proteins retain an extraordinary residual conformational entropy in the folded state, which provid

171 antibodies that specifically recognise a new conformational epitope formed by two different gangliosi

172 lectively to activated B cells, recognizes a conformational epitope on CD81 that is masked when CD81

173 nanoparticles that can preserve and present conformational epitopes of protein antigens for inductio

174 hallenge model identified critical antigenic conformational epitopes on H5 hemagglutinin (HA) from di

175 that uses the temperature-dependent loss of conformational epitopes to measure thermostability of GP

176 ibitors, including large-scale shifts in the conformational equilibria of the regulatory domains.

177 The conformational equilibria should therefore be an importa

178 e kinetics of re-equilibration to the native conformational equilibrium can be determined by time-res

179 267 conformational equilibrium constants measured across ele

180 d the impact of IP(6) on oligomerization and conformational equilibrium of the highly homologous arre

181 roach by designing a mutant that inverts the conformational equilibrium of the HIV-1 transactivation

182 identify specific residues involved in this conformational exchange that are highly conserved among

183 Two active site loops undergo large conformational excursions during this dynamic gating mec

184 ta show that binding of one agonist leads to conformational flexibility and an increase in conformati

185 mutations are all mapped onto the regions of conformational flexibility and functional importance, in

186 oped over the years in order to modulate the conformational flexibility and the peptide character of

187 l structural integrity of antigens and local conformational flexibility at antigenic sites, which may

188 the electrostatic environment due to protein conformational flexibility enables direct excitation of

189 The insert region has larger conformational flexibility in the GDP-bound Cdc42 than i

190 Together, these data suggest that sigma1 conformational flexibility modulates the efficiency of r

191 detailed characterization of changes in the conformational flexibility of EF-G in response to FusB b

192 se in protein multidomain rigidity, reducing conformational flexibility of F-actin-binding domains vi

193 The conformational flexibility of Y731 and the hydrogen-bond

194 PO monomeric and oligomeric states and their conformational flexibility, together with ligand-binding

195 It exhibits substantial conformational flexibility.

196 S exhibits extensive conformational flexibility: it modulates exposure of its

197 gh-resolution structures exist for AcrB, its conformational fluctuations and their putative role in f

198 binding motifs, our results suggest that the conformational fluctuations in a scaffold protein play a

199 proposed coupling between intra-/interdomain conformational fluctuations is a consequence of the dyna

200 We find not only that conformational fluctuations play a major role but also t

201 tions on assembly and identify four distinct conformational forms of the complex II flavoprotein that

202 The conformational free energy landscapes of free alpha-l-ar

203 f immunoglobulin genes and recognize various conformational HBsAg epitopes.

204 The loss of conformational heterogeneity and related functional dive

205 nal assays to investigate the role played by conformational heterogeneity in determining the activity

206 ity to the CCS distributions, reflecting the conformational heterogeneity of proteins and also how co

207 We find that it displays conformational heterogeneity with various elliptical sha

208 ate and multistate conformational ensembles, conformational hierarchies, osmolytes, coupling between

209 To better understand the conformational impact by the knob and hole mutations, we

210 elative contributions of configurational and conformational imprinting.

211 uding rostrocaudal relative distribution and conformational indicators, were maintained in sCJDVV1-2

212 imple-to-use analytical tools to monitor the conformational integrity of therapeutic-proteins during

213 These reagents exhibit sensitivity to the conformational integrity of various forms of rHuEPO and

214 lity, we also identified four initiating 48S conformational intermediates, provided novel insights in

215 oluminescence, contributes to the concept of conformational isomerism in nanoclusters, and demonstrat

216 ributed to field heating and the concomitant conformational isomerization.

217 addition to standard HPLC and MS analysis of conformational isomers of disulfide-rich peptides and pr

218 cs (MD) simulations to characterize the CheY conformational landscape accessed by FliM(N) and D13K-Y1

219 NAC co-binds with and remodels the conformational landscape of SRP on the ribosome to regul

220 roles, we offer an unprecedented view of the conformational mechanics of the knot and its relationshi

221 the intra-N disulfide bonds, which restrict conformational motion within the N-domain, prevented FeE

222 ell as the hydrogen-bonding interactions and conformational motions of interfacial residues along the

223 Here, we present chemically-detailed conformational movies of biological function, extracted

224 ation ensembles toward functionally distinct conformational or dynamic states.

225 indicate a progressive increase in the chain conformational order with increasing n between n = 6 and

226 somes was characterized (bilayer size, chain conformational order, lateral packing, lipid phase, and

227 ty fits than the deposited primary/alternate conformational pairs.

228 inding assay) to investigate the role of DNA conformational penalties in transcription factor-DNA rec

229 The conformational plasticity of IL-2 plays an important rol

230 t metal ion per C2 domain results in loss of conformational plasticity of the loop regions, potential

231 riving forces and reveals granular detail of conformational populations in space and time.

232 onding interaction energetics of billions of conformational poses were ranked by the programs PIPER a

233 aSyn-mediated vesicle fusion by altering the conformational properties of the protein when lipid boun

234 ules possessing interesting and well-defined conformational properties.

235 The conformational ratio of the (1) C(4) :(2) S(0) forms of

236 Here, we focus on a conformational rearrangement during translocation, known

237 diating the association kinetics, undergoing conformational rearrangement upon initial binding.

238 This conformational rearrangement was observed both in deterg

239 the alpha5-alpha6 linker that likely mediate conformational rearrangements in the protein and cognate

240 These data suggest that sigma1 conformational rearrangements mediate the efficiency of

241 These outcomes indicate that conformational rearrangements must occur in the N termin

242 sion in protein systems could facilitate the conformational rearrangements required to create hierarc

243 arison of these structures reveals concerted conformational rearrangements through which the HA media

244 Icosahedral viral capsids must undergo conformational rearrangements to coordinate essential pr

245 und that menthol binding induces wide-spread conformational rearrangements within the transmembrane d

246 The conformational rigidity and the enriched stability of th

247 tial coverage of the target ligand, performs conformational sampling and template-guided energy refin

248 Understanding the conformational sampling of translation-arrested ribosome

249 The modelled complexes were subjected to conformational sampling using the Backrub method in Rose

250 are also affected by calreticulin through a conformational selection mechanism that facilitates MHC-

251 c and computational studies that show that a conformational selection mechanism, in which POT1 bindin

252 activated DNA binding by HpNikR is driven by conformational selection.

253 tly decreases the duplex stability through a conformational shift of native Watson-Crick pair to a wo

254 nges at specific sites within the loop and a conformational shift of the loop during activation.

255 cellular environment, we wanted to model the conformational space adopted by the PRD.

256 The result shows that a viable conformational space is predominantly determined by the

257 chniques depends on how much of the relevant conformational space of a particular compound is conside

258 also made it possible to explore the entire conformational space of these nanomachines under turnove

259 region of a target can be used expanding the conformational space sampled in a meaningful way.

260 In doing so, the capsids sample a range of conformational space, with implications for maintaining

261 onformational flexibility and an increase in conformational spread at the second agonist site.

262 2 provided hints of the remarkably different conformational stability and substrate specificity.

263 The specific structural, conformational, stability, reactivity and interaction fe

264 ry under low ionic conditions and revealed a conformational state at intermediate ionic conditions pr

265 The application of smFRET to assess the conformational state of existing Env constructs and liga

266 The application of smFRET to test the conformational state of existing Env constructs and liga

267 that are sufficient to characterize a minor conformational state of the C-terminal region of DHFR.

268 How substrate binding influences the conformational state of VcINDY is currently unknown.

269 iction methods lack an analysis of the major conformational states and might underestimate the impact

270 nning different levels of classification and conformational states and totaling to 1 ms of simulation

271 T via a more complex mechanism involving two conformational states interconverting on the second time

272 ificial target data reflecting the two major conformational states of a binding protein.

273 ave been invaluable tools to isolate defined conformational states of proteins, and we reasoned that

274 ered in residues that participate in dynamic conformational states of sarcomere proteins.

275 structural biology by identifying transient conformational states that are highly abundant in biolog

276 ansport by Atm1 may involve a limited set of conformational states with minimal separation of the nuc

277 02A1 is highly dynamic, existing in multiple conformational states, including open and closed states.

278 article cryo-electron microscopy in distinct conformational states, stabilized by individual disulfid

279 c constants provide glimpses of intermediate conformational states, time scales for motion, and assoc

280 signs of chemical shift changes between the conformational states, which are difficult to obtain in

281 nd disassembly of protein aggregates in both conformational states.

282 analysis of endogenous proteins in discrete conformational states.

283 es have been proposed to represent different conformational strains of alpha-synuclein that can self-

284 ifferent way and lead to remodeling of their conformational substates.

285 ucose 1-phosphate concentration, couples the conformational switch and the phosphorylation step, resu

286 We reveal that a single conformational switch by ATP binding drives unidirection

287 interaction with cholesterol may regulate a conformational switch in the large extracellular domain

288 propose that the extension domain acts as a conformational switch with differential response options

289 sted that this proton binding also prompts a conformational switch, leading to a loss of affinity for

290 CD81 interactome analysis also suggests that conformational switching may modulate the assembly of CD

291 atively bind to actin, thus facilitating Tpm conformational switching to cooperatively propagate alon

292 ne environment, revealing putative sites for conformational switching.

293 entry inhibitor BMS-626529 restricts trimer conformational transition and improves the immunogenicit

294 yrococcus horikoshii suggested that the slow conformational transition from the outward- to the inwar

295 nsively and to elucidate their effect on the conformational transition.

296 lytical approaches that can identify protein conformational transitions and simultaneously characteri

297 he gut, have several structural features and conformational transitions that distinguish them from ot

298 s, moss, and green algae can undergo dynamic conformational transitions, which have been correlated t

299 ates viral-host cell fusion via a network of conformational transitions, with allosteric elements in

300 t curvature in the CA tubes is controlled by conformational variability of residues in the linker reg