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6 t Tn7 is attracted to events associated with conjugal DNA replication during plasmid DNA transfer.
7 in a functional icm complex that can mediate conjugal DNA transfer and probably can initiate infectio
8 ass of transfer factors that carry genes for conjugal DNA transfer and, in some cases, antibiotic res
23 cins are powerful counterselective agents in conjugal DNA transfers using colicin-sensitive Escherich
25 4a tra operons is not activated by TraR, and conjugal efficiency is not affected by TraR and 3-oxo-C8
28 nomes has revealed an extraordinary array of conjugal elements (integrative and conjugative element o
29 novel elements, designated ICEF (integrative conjugal elements of M. fermentans), resemble conjugativ
38 ne by itself, rather than the formation of a conjugal intermediate with pSa, blocks transformation su
39 tudy was to describe the association between conjugal loss and both syndromal depression and depressi
41 ith plasmid R1162 DNA were used as donors in conjugal matings to determine if the plasmid replication
44 pSC101 and R1162 encode related systems for conjugal mobilization, although these plasmids are other
45 clinics, we have determined the frequency of conjugal MS and have estimated the recurrence risk in of
49 donors grown with agrocinopines A and B, the conjugal opines for this Ti plasmid, was detected only a
51 s support the hypothesis that nicking at the conjugal origin initiates the recombination that produce
52 Within strain pairs, targeted placement of a conjugal origin of transfer and selectable markers in do
53 he production of a single-strand nick at the conjugal origin, we supplied an exogenous nicking enzyme
56 rity in crude recurrence between children of conjugal pairs and those of single affected parents show
62 cell subpopulation that does not transcribe conjugal plasmid transfer genes, which may help reduce p
65 chromosome or replicating DNA structures in conjugal plasmids, ensuring the vertical and horizontal
69 -system family member ESX-4 is essential for conjugal recipient activity in Mycobacterium smegmatis T
71 opines and the corresponding opine-meditated conjugal regulatory regions of pTiChry5 and pTiC58 share
74 cleaving both strands of the Tn916 origin of conjugal transfer (oriT) at several distinct sites and f
75 egion encoding plasmid replication (rep) and conjugal transfer (tra) functions similar to those encod
76 However, these mutants retained residual conjugal transfer activity when tested in strain NT1, wh
78 RP4 (TraG(RP4)) and TraG and VirD4 from the conjugal transfer and T-DNA transfer systems of Ti plasm
79 sing system, suggesting a connection between conjugal transfer and vegetative replication of these vi
81 For low numbers of sites, the efficiency of conjugal transfer decreased as an exponential function o
82 port that the Agrobacterium-to-Agrobacterium conjugal transfer efficiencies of RSF1010 increase drama
83 plasmid TiC58 or plasmid RSF1010 reduces the conjugal transfer efficiency of pAtC58 10- or 1,000-fold
84 he quorum-dependent activation of Ti plasmid conjugal transfer exhibited a lag of almost 8 h when the
87 bB, -C, -D, -E, -L, -F, -G, and -H abolished conjugal transfer from strain UIA5, which lacks the 450-
89 nosa and Erwinia spp., as well as T1 plasmid conjugal transfer in Agrobacterium tumefaciens, and many
91 ed may be used to increase the efficiency of conjugal transfer into restriction-competent bacteria.
92 lasmid gene markers: traA from the conserved conjugal transfer machinery and vapA and vapB, found in
95 es (close to 100%) from the chromosome after conjugal transfer of a Flp recombinase-expressing plasmi
102 s well as its own synthesis, can promote the conjugal transfer of both chromosomal and plasmid genes
104 hines, but present-day functions include (i) conjugal transfer of DNA by cell-to-cell contact, (ii) t
108 tudies did not detect replication delays for conjugal transfer of episomes containing PIT elements.
109 nd proteins into plant cells, as well as the conjugal transfer of IncQ plasmids, such as RSF1010, bet
111 ced stimulatory effect on the virB-dependent conjugal transfer of plasmid RSF1010 by agrobacterial do
118 the first time an oriT, which is involved in conjugal transfer of the circular form, has been implica
119 cell, while the other is responsible for the conjugal transfer of the entire Ti plasmid from one bact
121 e the VirB system, AvhB products promote the conjugal transfer of the IncQ plasmid RSF1010, suggestin
122 for tumor formation; rather, it mediates the conjugal transfer of the pAtC58 cryptic plasmid between
123 e major type IV pilin subunit (pilS) reduced conjugal transfer of the plasmid by 4 orders of magnitud
124 um tumefaciens, activates genes required for conjugal transfer of the Ti plasmid and also enhances th
125 rb system encodes functions required for the conjugal transfer of the Ti plasmid between cells of Agr
129 3-oxo-octanoyl-l-homoserine lactone control conjugal transfer of the tumor-inducing plasmid, the pri
130 iption factor TraR regulates replication and conjugal transfer of the tumour-inducing (Ti) plasmid in
131 efaciens, which controls the replication and conjugal transfer of the tumour-inducing (Ti) virulence
132 ine-type Ti plasmid pTiC58 are important for conjugal transfer of this element to recipient bacteria.
136 genetic transformation of M. arthritidis and conjugal transfer of Tn916 from an enterococcal donor to
137 be part of a mechanism to prevent premature conjugal transfer of Tn916 prior to excision from the do
142 ssociated with the type IV secretion system, conjugal transfer protein TraG, plant-pathogen interacti
143 system enables A. tumefaciens to express its conjugal transfer regulon preferentially at high populat
144 ce and appears to constitute components of a conjugal transfer system that has been adopted to preven
147 taining a coresident conjugative transposon, conjugal transfer was repressed in the absence of tetrac
148 that a negative and a positive regulator of conjugal transfer were encoded outside the transfer regi
150 mids encode genes involved in pilus-mediated conjugal transfer, as well as pilL-V, which encodes a se
151 uency of transposon loss is a consequence of conjugal transfer, making this loss a marker for that tr
152 P plasmid was cloned into pGNS-BAC to enable conjugal transfer, thereby allowing both electroporation
153 ring excision and nicking at the oriT during conjugal transfer, to prevent premature nicking at the o
154 ing single strand in the recipient, early in conjugal transfer, until lagging-strand replication crea
155 hat at least traG and traF are essential for conjugal transfer, while sequence analysis predicts that