ライフサイエンスコーパス: conserved region

1 esided within a 7 amino-acid, evolutionarily conserved region.

2 level variability across the entire central conserved region.

3 rotein complexity and identifying functional conserved regions.

4 sed patterns/motifs tethered together by non-conserved regions.

5 the interaction of pRb family proteins with conserved region 2 (CR2) of E1A is known to promote cell

6 ce of a unique spacer region located between conserved regions 2 and 3 within the HAdV-A12 early regi

7 Ca(2+)-dependent conserved-region 2 (C2) domains target their host signal

8 y islet transcription factors binding within conserved Region 3 (base pairs (bp) -8118/-7750) of MafA

9 We show that Par3 conserved region 3 (CR3) forms a tight inhibitory comple

10 ription, and we revealed that in addition to conserved region 3 (CR3) of FOXO3a, the C-terminal segme

11 tor sigmaA of M. tuberculosis containing the conserved region 4 (sigmaA4).

12 conserved template/pseudoknot domain, and a conserved regions 4 and 5 (CR4/5) domain, all essential

13 ood gene expression: coding regions (4.47x), conserved regions (4.51x), and two histone marks (p < 3

14 expression included coding regions (11.41x), conserved regions (4.67x), and four histone marks (p < 5

15 nserved element was discovered (evolutionary conserved region 47) that functions as a transcriptional

16 -variant ADHD risk haplotype in evolutionary conserved region 47, formed by rs17226398, rs56038622, a

17 nclude a large enrichment of heritability in conserved regions across many traits, a very large immun

18 urther identify a glutamate residue within a conserved region adjacent to the last two putative TM do

19 and binding of RNA polymerase II within this conserved region, all of which are consistent with trans

20 We sequenced highly conserved regions along 22q from eight individuals with

21 This highly conserved region also makes contacts with residues on he

22 the identification and characterization of a conserved region, also known as H10, in intron IV of the

23 loop ((108)FQRFTPFTLGKEFKE(123)G), a highly conserved region among eAs that mediates binding to thei

24 The neck-linker is a structurally conserved region among most members of the kinesin super

25 mmon feature of MASPs is the presence of two conserved regions: an N-terminal region codifying for si

26 A reveals that plant-specific modules (plant-conserved region and class-specific region) fold into di

27 The conserved region and flanking sequences in an 85-bp intr

28 results support the importance of the plant-conserved region and/or class-specific region in CESA ol

29 s to C-rich single-stranded DNA within these conserved regions and also associates with double-strand

30 This complex, stabilized by cytosolic plant-conserved regions and helical exchange within the transm

31 n of HIV-specific T-cell responses targeting conserved regions and induction of HIV-specific T-cell r

32 GNA finds large conserved regions and produces a one-to-one node mapping

33 ion factors while residing in evolutionarily conserved regions and resulting in expression changes of

34 ions link previously identified structurally conserved regions and reveal new motifs, which are beyon

35 indicating similar immunogenicities for the conserved regions and the outside regions.

36 emonstrate the immunogenicity of these small conserved regions and the potential for a vaccine to ste

37 says for C. watsonii abundances targeted the conserved regions and therefore could not differentiate

38 The alignment is arranged in 17 blocks of conserved regions and unaligned blocks in between that c

39 Two conserved regions are central for selective function: th

40 ncoding regulatory elements; however, extant conserved regions are enriched for novel noncoding RNAs

41 these findings demonstrate that the selected conserved regions are highly immunogenic and that anatom

42 helical bundle reveal critical roles for the conserved region as well as helix five.

43 : antibodies present in the antisera bind to conserved regions associated with cross-neutralization.

44 These data suggest that a conserved region at 20 kb upstream from the MMP-13 TSS i

45 , located in a non-coding but evolutionarily conserved region at 8q24.21, is strongly associated with

46 movirus L consisting of CR-VI and the poorly conserved region at its C terminus, the +domain.

47 A conserved region at the 3' end of the locus, containing

48 Additionally, an analysis of highly conserved regions at the family level revealed molecular

49 modes at recombination signal sequence (RSS)-conserved regions before nicking and synapsis.

50 protein interaction studies, we found that a conserved region between amino acids 34 and 52 of NleE,

51 nds on the most C-terminal coiled-coil and a conserved region between this coiled-coil and the transm

52 Network alignment aims to find conserved regions between different networks.

53 Sequence alignment revealed several highly conserved regions between T-type and Na(v) channels that

54 Network alignment (NA) finds conserved regions between two networks.

55 Conserved region C (CR-C), a promoter proximal cis-regul

56 active to an epitope proximal to the central conserved region (CCR) of RSV G protein (mAb 131-2G) sho

57 c analyses showed that eSTRs are enriched in conserved regions, colocalize with regulatory elements a

58 Since the tHIVconsvX vaccine targets conserved regions common to most global HIV-1 variants a

59 All members share a conserved region composed of five transmembrane regions.

60 SNPs in less conserved regions contributed more to occipital and dors

61 lso observe that SNPs in evolutionarily more conserved regions contributed significantly to the herit

62 nds on the specific MIRNA We show that these conserved regions correspond to structural determinants

63 The coiled coil has two spatially distinct conserved regions: CRI and CRII.

64 orter construct driven by a component of the conserved region demonstrated an expression pattern simi

65 The poorly conserved region distal to the phosphorylation cluster i

66 insertional duplication of an evolutionarily conserved region (ECR) downstream of Hmx1 has recently b

67 sis, we have identified three evolutionarily conserved regions (ECRs), alpha, SINA, and DINA, that ac

68 nonneutralizing antibodies binding to a less conserved region encompassing residues 434 to 446.

69 and biophysical analyses suggested that the conserved region forms a regular secondary structure ado

70 the periphery of the complex, and the plant-conserved region forms the base of the trimer.

71 n in-frame deletion mutation (DeltaE81) in a conserved region from the first ubiquitin interaction mo

72 ned the synthetic gene '5GHPV3' by selecting conserved regions from each of the six early proteins an

73 Thus, FALCON@home first extracts conserved regions from each template and then aligns a q

74 nces; therefore, we selected four relatively conserved regions (Gag amino acids 148 to 214 and 250 to

75 ntifying rSNPs focus on sequence variants in conserved regions; however, it is unknown what fraction

76 sent the structure of the N-terminal domain (conserved region I) of the L protein from a rhabdovirus,

77 high yielding soluble fragments encompassing conserved regions I and II (CRI and CRII) at the C-termi

78 Mutations in four GAAG repeats within the conserved region impaired generation of the same splice

79 part of the Spp2 G-patch, which is the most conserved region in different G-patch proteins, transien

80 Here we identify a conserved region in DRC4 critical for assembly of the N-

81 Strikingly, a conserved region in Dsup proteins exhibits sequence simi

82 PB1 through a single guide RNA recognizing a conserved region in exon 2.

83 d (CA) HIV-1 DNA and RNA, targeting a highly conserved region in HIV-1pol, with sensitivities of 3 to

84 S349 and S351 are located in a conserved region in the C-terminal domain of YopJ family

85 minus of Rav1, V1 subunits E and C bind to a conserved region in the C-terminal half of Rav1, and the

86 ch indicated binding of miR-1247 to a highly conserved region in the coding sequence of SOX9 but not

87 haracterized the function of the only highly conserved region in the H2B tail, the H2B repression (HB

88 A domain called the 'Conserved region in the middle' is responsible for targe

89 In this study, a highly conserved region in the N terminus of FMDV capsid protei

90 The TRP box, a conserved region in the N-end of the C terminus domain,

91 One of the tested MAbs, which binds to a conserved region in the NA of pH1N1 virus, inhibited NA

92 , we demonstrate that SOX3 binds to a highly conserved region in the Ngn3 promoter region in vivo, in

93 We identify a conserved region in the Ulp2 C terminus that mediates it

94 yces cerevisiae Upf2 and identified a highly conserved region in this domain that is essential for NM

95 entral part of HaNLP3 and corresponding to a conserved region in type 1 NLPs that induces ethylene pr

96 Mapping of conserved regions in a set of CagA sequences identified

97 targeted proteome areas outside the selected conserved regions in direct proportion to sequence lengt

98 The number of conserved regions in plant introns appeared to be hundre

99 We identify a NELF core subcomplex formed by conserved regions in subunits NELF-A and NELF-C, and res

100 s, such as pleconaril and vapendavir, target conserved regions in the capsids of rhinoviruses (RVs) a

101 We described conserved regions in the endoplasmic reticulum-facing lo

102 s9 system can specifically target and cleave conserved regions in the HBV genome, resulting in robust

103 most active shRNA molecules against the most conserved regions in the HCV genome, which were then use

104 Furthermore, targeting a set of five conserved regions in the HIV-1 gag gene, the method coul

105 We show that Aiolos binds conserved regions in the IL10 locus in IL-17+ CD4+ T cel

106 We propose cholesterol interacts with the conserved regions in the LAT1 transporter that have been

107 othalamus is among the most phylogenetically conserved regions in the vertebrate brain, reflecting it

108 ersity and primer localization in relatively conserved regions, in addition to recognized constraints

109 r restricts access to the active site as its conserved-region-in-the-middle (CRIM) domain is position

110 Conserved regions include the IR1 latency promoter (Wp)

111 for remote homologous proteins, consists of conserved regions interweaved with highly variable regio

112 complete rescue suggests that while the core conserved region is essential, additional sequences cont

113 We found that this conserved region is necessary and sufficient for binding

114 bodies specific for region III or the highly conserved regions IV-V predict strong protection from cl

115 mentation between the zinc finger domain and conserved region IX.

116 SH2) domain of p85 through an evolutionarily conserved region located at the C terminus of BRD7.

117 uence flanking to the K1 segment and a novel conserved region located between the S and K1 segments [

118 To elicit antibodies against conserved regions located on capsid proteins of RV A vir

119 ccine-elicited immune responses or targeting conserved regions may improve coverage of circulating st

120 ellow fever, and West Nile virus, identifies conserved regions modified by m(6)A.

121 that noroviruses often recombine in a highly conserved region near the junction of the viral polyprot

122 s M10-I17, which is included within a highly conserved region near the N-terminal.

123 Mutations within this conserved region not only inactivate NMD but also disrup

124 omologue of NSUN5, methylates C2278 within a conserved region of 25S rRNA.

125 Alignment of amino acid sequences revealed a conserved region of 52 amino acids, referred herein as W

126 polymorphisms, one of which was in a highly conserved region of a gene encoding a sensor kinase, lia

127 neutralizing antibodies (bNAb) that target a conserved region of a viral antigen hold significant the

128 We report here that the most strongly conserved region of Aha-type co-chaperones, the N termin

129 multiple sites near the D1 domain, a highly conserved region of bacterial flagellins that interact w

130 n the 'RNR' region, which represent the most conserved region of bacterial RNase P proteins, exhibit

131 A conserved region of E2 encompassing amino acids 412 to 4

132 s work, we examined the role of PNLGs in the conserved region of HIV-1 Env, particularly the role of

133 Herein we demonstrate that a conserved region of human and mouse SYN1 promoters conta

134 The conserved region of influenza hemagglutinin (HA) stalk (

135 ing qRT-PCR primers and a probe for a highly conserved region of integrase in the HIV-1 pol gene (the

136 tion resulted in alternative splicing in the conserved region of intron 34, which strongly decreased

137 miR-30, with three binding sites in a highly conserved region of its 3' UTR, operates as repressor of

138 identified an A896T substitution in a highly conserved region of KCNJ2 that resulted in a de novo mut

139 We identify a new conserved region of KIBRA.

140 4-restricted T-cell epitopes within a highly conserved region of membrane protein, which induced poly

141 closely related to mule deer CrERVgamma in a conserved region of pol; more distantly related sequence

142 a putative catalytic residue within a highly conserved region of RbgA, His9, which is contained withi

143 divergent open reading frames (ORFs) in the conserved region of rpoA, the gene encoding the PEP alph

144 We also discovered a highly conserved region of She3p upstream of its hook region.

145 A second conserved region of Stepping stone was necessary and lar

146 turing; the findings do not exclude the core conserved region of TapA having a second role beyond str

147 y all of DmSNAPc, a portion of Bdp1, and the conserved region of TBP.

148 hat the CP2 motif is in close proximity to a conserved region of TER known as the template boundary e

149 erase essential N-terminal (TEN) domain is a conserved region of TERT proposed to mediate DNA substra

150 ), and a CD4 or CD8 molecule that binds to a conserved region of that same MHC gene product (class II

151 ptase quantitative PCR (RT-qPCR) targeting a conserved region of the 5' untranslated region (5'-UTR).

152 ation is located in an evolutionarily highly conserved region of the alpha-helical rod of the lamin B

153 ucture of the heterodimer of the multidomain conserved region of the DBHS proteins, PSPC1 and NONO.

154 nteracting with amino acid residues within a conserved region of the DNA binding domain.

155 ecently solved crystal structure of a highly conserved region of the Drosophila orthologue of BICD2 t

156 on of a single tyrosine (p*Y504) in a highly conserved region of the fibronectin type III (FN3) domai

157 OCHROME C (HvPHYC), carrying a mutation in a conserved region of the GAF domain, is a candidate under

158 This variant is in exon 2, a highly conserved region of the gene.

159 OMA-1 binds with high affinity to a conserved region of the glp-1 3'-UTR previously shown to

160 The most conserved region of the HIV type 1 (HIV-1) genome, the a

161 orthogonal methods, alters an evolutionarily conserved region of the HMGB3 protein from a negatively

162 We also show that four genes within the more conserved region of the KSHV genome contained polymorphi

163 sociation is mediated through the N-terminus conserved region of the LFY protein.

164 A synthetic peptide vaccine (J8-DT) from the conserved region of the M protein has shown efficacy aga

165 YBR Green chemistry with primers targeting a conserved region of the M1 gene of influenza A viruses).

166 ively in yeast, and define an evolutionarily conserved region of the N domain involved in resetting t

167 e identification of pathogenic variants in a conserved region of the OVOL2 proximal promoter sequence

168 S690 is in a conserved region of the p85 cSH2 domain, and IKK-mediate

169 Although this domain of AF10 is the most conserved region of the protein, its biological signific

170 , and we determined association of ELF3 to a conserved region of the PRR9 promoter.

171 ovel missense mutation (p.I141V) in a highly conserved region of the SCN5A gene, encoding the Nav1.5

172 tional Formica species reveals that the most conserved region of the supergene contains a transcripti

173 ural progenitor cells of the hypothalamus, a conserved region of the vertebrate brain that centrally

174 The epithalamus is a highly conserved region of the vertebrate brain that differenti

175 A therapeutic agent that targets a highly conserved region of the virus could bypass resistance an

176 telet dysfunction, caused by the defect in a conserved region of the VPS33A gene on human chromosome

177 ulosis at ambient temperatures and detects a conserved region of the Zika virus after isothermal nucl

178 interference construct, designed to a highly conserved region of these genes, was used to transform c

179 contain multiple amino acid changes within a conserved region of VHS.

180 novel frame-shift insertion discovered in a conserved region of WNT16a, and it is proposed that this

181 enly map to reference genomes, especially to conserved regions of common housekeeping genes, such as

182 results lend support for the development of conserved regions of EBA-175 as components in a combinat

183 bsence of glycosylation sites encoded in the conserved regions of env, further reduced in transmitted

184 Because eCD4-Ig binds only conserved regions of Env, it is also much broader than a

185 cted subjects that target other structurally conserved regions of Env.

186 Six distinct NAIP5 domains contact multiple conserved regions of flagellin, prying NAIP5 into an ope

187 LIPs, and that subtle differences within the conserved regions of FLIPs possess distinct molecular an

188 that elicit broadly neutralizing Abs against conserved regions of HA, such as the stalk domain.

189 s) can greatly assist others in highlighting conserved regions of hemagglutinin.

190 e HIV-1 isolates and if other glycans in the conserved regions of HIV-1 Env display similar functions

191 However, the role of specific glycans in the conserved regions of HIV-1 Env in modulating epitope rec

192 reverse transcriptase (RT)-PCR detection of conserved regions of HIV-1 RNA and can be limited by det

193 ADCC-mediating Abs can target more conserved regions of influenza virus proteins and recogn

194 ines against influenza virus, elucidation of conserved regions of influenza viruses is crucial.

195 t-binding site(s) on RD3, short evolutionary conserved regions of its primary structure were scramble

196 d recognize immunodominant viral epitopes in conserved regions of membrane, spike, and nucleocapsid.

197 polymerase chain reaction (PCR) assay using conserved regions of mitochondrial DNA (cytochrome b gen

198 o methionine substitution (I4790M) in highly conserved regions of RyR.

199 ty by RbpA, showing that RbpA interacts with conserved regions of sigma(A) as well as the nonconserve

200 We examined the functional significance of conserved regions of SmpB and tmRNA to the trans-transla

201 (Trp-501) region of DdMII demonstrate these conserved regions of the active site are sensitive to Mg

202 domains that roughly correspond to the three conserved regions of the CTD: an alpha helix, needed for

203 ertype-restricted immune responses to highly conserved regions of the dengue proteome may protect aga

204 As N-linked glycans are thought to shield conserved regions of the EBOV GP receptor-binding domain

205 Skewing away from these conserved regions of the epitopes recognized by current

206 mosaic T-cell vaccine immunogens composed of conserved regions of the Gag and Pol proteins matched to

207 possible, though there are no suitable 100% conserved regions of the gene, leading to bias.

208 moval of the heavy glycan shield surrounding conserved regions of the glycoprotein does not enhance v

209 velopment is focusing the immune response on conserved regions of the HCV envelope protein, E2, capab

210 n mice to boost the humoral response against conserved regions of the hemagglutinin (HA) glycoprotein

211 e monoclonal antibody designed to target the conserved regions of the hemagglutinin of influenza A H1

212 ation-scale boost in antibodies specific for conserved regions of the hemagglutinin stalk and the vir

213 t advantage to simultaneously targeting both conserved regions of the HIV-1 envelope glycoprotein.

214 induction of neutralizing antibodies against conserved regions of the human immunodeficiency virus ty

215 ivating CD8+ and CD4+ T cells against highly conserved regions of the influenza genome.

216 la patens and identified mutations in highly conserved regions of the moss ortholog of tomato DGT.

217 tasks are likely subserved by evolutionarily conserved regions of the nervous system.

218 s a series of basic residues residing on two conserved regions of the primary structure.

219 his extensive glycosylation not only shields conserved regions of the protein from the immune system

220 ocated near catalytic sites or within highly conserved regions of the protein.

221 ingle mutations localize within three highly conserved regions of the telomerase thumb domain referre

222 In order to assess the importance of conserved regions of the UL15 and UL28 proteins, recombi

223 iscover de novo functional motifs located in conserved regions of the viral genome.

224 earch has focused on the potential of highly conserved regions of the viral hemagglutinin (HA) as tar

225 T-PCR) with degenerate primer sets targeting conserved regions of the viral L genome segment.

226 Allosteric ligands may bind to less-conserved regions of these receptors and therefore are m

227 ither deletions or substitutions in the most conserved regions of UL33 using a bacterial artificial c

228 dence suggests that HLAs preferentially bind conserved regions of viral proteins, a concept we term "

229 ed two previously unannotated evolutionarily conserved regions of ZIC3 that are necessary for trans-a

230 This work identifies a conserved region on the C terminus of Zip1 (called Zip1

231 potent inhibiting antibodies specific for a conserved region on the globular head of the pandemic H2

232 utoreactive bNAbs, 2F5 and 4E10, recognize a conserved region on the HIV-1 envelope glycoprotein gp41

233 tructural and biochemical studies identify a conserved region on TREX-2, which directly binds the Med

234 spectrum of viruses by targeting relatively conserved regions on the surface HIV envelope trimer spi

235 arise during natural HIV-1 infection bind to conserved regions on the virus envelope glycoprotein and

236 emplate and then aligns a query protein with conserved regions only rather than the full-length templ

237 h of cellular immune responses compared with conserved-region-only HIV-1 immunogens, including greate

238 fic T lymphocyte responses compared with the conserved-region-only immunogens (P = 0.007).

239 st conserved HIV-1 regions compared with the conserved-region-only immunogens.

240 Inactivating mutations in this conserved region or in p10 can inhibit activation of the

241 roup (HMG) box DNA-binding domain and poorly conserved regions outside the HMG box.

242 ation of PsbZIP1 was determined by a central conserved region overlapping the DNA binding domain.

243 a sativa) cellulose synthase, OsCesA8, plant-conserved region (P-CR), one of two unique domains in th

244 unstudied despite being located in a highly conserved region proximal to the proteolytic site.

245 raction of the genome than do evolutionarily conserved regions, raising the question of whether nonco

246 Although the conserved regions ranged from 43 to 86 amino acids and c

247 homologs but also identified a novel, highly conserved region (region IX) essential for terminase fun

248 putative zinc finger motif (30-ZF3A) or in a conserved region (region IX) with predicted structural s

249 monstrated by the prevalence of structurally conserved regions (SCRs) even in highly divergent protei

250 The structure reveals that two highly conserved regions, separated by an unconserved linker, f

251 Truncation analyses of the non-conserved regions showed that they are not essential for

252 Genetic variants spanning evolutionarily conserved regions significantly explained 92.4% of Toure

253 Baseline enhancer activity of the conserved region specifically on the MYC promoter and it

254 The antibody binds to RSV G at a highly conserved region stabilized by two disulfide bonds, but

255 ' untranslated region containing five highly conserved regions suggesting miRNA regulation of its exp

256 to variable antigenic sites or structurally conserved regions, suggesting a combination of evolution

257 combined with frame-disrupting mutations in conserved regions, suggests that evolutionary pressure p

258 Vaccination with J8, a conserved region synthetic peptide derived from the M-pr

259 The CRV yielded a conserved-region targeting density that was approximatel

260 They interact with less-conserved regions than known capsid binders.

261 The survivin protein forms dimers through a conserved region that is critical for subcellular locali

262 ions were missense and presented in a highly conserved region that is implicated in ATP catalysis.

263 We find that Vms1 contains a highly conserved region that is necessary and sufficient for mi

264 The DRS is a conserved region that lies distal to the active site and

265 nal transmembrane domain of CHS1 in a highly conserved region that may serve a noncatalytic but essen

266 terminal to the ankyrin repeats, in a highly conserved region that we identify here as a novel domain

267 vaccine serotypes to locate primer pairs in conserved regions that border variable regions and could

268 n-mediated endocytic receptors contain short conserved regions that recognize tyrosine or dileucine s

269 We show that one of these conserved regions, the p62/Tfb1 Anchor region, directly

270 ha-helix that potentiates the ability of the conserved region to self-assemble.

271 nteracts with Ldb as well as Awh through the conserved regions to mediate transcriptional activation

272 An upstream conserved region (UCR) localizes Sec16 to tER sites.

273 in which each subunit contains two upstream conserved regions (UCR), UCR1 and -2, which precede the

274 A new method that can identify uniquely conserved regions (UCRs) as candidate diagnostic targets

275 of 140 sequences of PIB-1-ATPases revealed a conserved region unusually rich in histidine and cystein

276 impact on activation efficiency, whereas the conserved region up to the first HXH motif is highly ben

277 A conserved-region vaccine (CRV) delivering genes for thes

278 The conserved region VI (CR VI) of the large (L) polymerase

279 The conserved region VI (CR VI) of the large (L) polymerase

280 While targeting of conserved regions was similar in the two groups (P = 0.4

281 A previous study using LDSC reported that conserved regions were 13-fold enriched, and found a fur

282 11 different conserved regions were identified, some corresponding to

283 3, Arg74, Lys79 and Arg81) in the downstream conserved regions were required for binding of WhiB1 DNA

284 One highly conserved region, which contained a myocyte enhancer fac

285 Its non-conserved regions, which are located between the conserv

286 g and ubiquitin-like domains, they contain a conserved region with similarity to retroviral proteases

287 Notably, we identified a novel conserved region with the consensus sequence WWxxRxxLD p

288 Our results suggest that targeting conserved regions with less plasticity and more structur

289 ri, which share 97% nucleotide identity over conserved regions with SPI-7 and with each other.

290 bp downstream of the termination codon, in a conserved region, with the A-allele ancestral in primate

291 s recently been identified to sense a highly conserved region within bacterial 23S rRNA.

292 These differences are mediated by a conserved region within the carboxy terminus of perilipi

293 the hMPV N protein that was the most highly conserved region within the hRSV N protein.

294 we determined that androgen receptor binds a conserved region within the phosphatase, Ptpn1, and cons

295 fied three such epitopes derived from highly conserved regions within LASV proteins.

296 Comparative phylogenetic analysis of highly conserved regions within Pezizomycotina TERs revealed tw

297 udy was to determine the contribution of two conserved regions within TbpB to the function of this pr

298 We examine 15 evolutionary conserved regions within the Jagged1 genomic locus and i

299 In addition, less conserved regions within the N terminus of Ets1 play an

300 h carries two amino acid changes to a highly conserved region, yields a dominant-negative phenotype t