1 r proteins (e.g., helicases, chelatases, and
copines).
2 ng properties similar to those of Paramecium
copine.
3 7, a secretory vesicle-binding protein, as a
copine.
4 in-protein interaction for the VWA domain of
copines.
5 of unknown function were identified, such as
Copine 1 and PICOT, whose developmental regulation was p
6 Mutation of the Arabidopsis CPN1 (
COPINE 1) gene causes a humidity-sensitive lesion mimic
7 volved in signalling, such as importin 7 and
copine 1, cytoplasmic intermediate filament (IF) protein
8 Genetic deletion or downregulation of
Copine-
6 in the DRG in vivo selectively impairs sensitiv
9 Copine-
6 interacts with TRPM3 via its von Willebrand fac
10 Copine-
6 is a calcium-sensitive phospholipid-binding pro
11 itivity range of the nerve fibers; moreover,
Copine-
6 is an accessible target for the treatment of he
12 Copine-
6 is expressed in peripheral somatosensory neuron
13 Copine-
6 is highly expressed in a subpopulation of dorsa
14 Here we demonstrate that
Copine-
6 plays a prominent role in thermosensation.
15 In the central nervous system,
Copine-
6 regulates function of some neurotransmitter rec
16 plasticity, including SynGAP1, Pak3, GEPH1,
Copine-
6, and collybistin, and DNA and chromatin remodel
17 Thus,
Copine-
6-dependent TRPM3 trafficking determines noxious-
18 In our previous study,
copine 7 (CPNE7) could induce dentin formation for an in
19 However, the
copine amino acid sequences lack the traditional kinase
20 The highest levels of
copine are found in the spleen.
21 The
copines are a newly identified class of calcium-dependen
22 The
copines are a novel group of Ca(2+)-dependent, phospholi
23 The
copines are a widely distributed class of calcium-depend
24 Copines are calcium-dependent membrane-binding proteins
25 Copines are calcium-responsive, phospholipid-binding pro
26 athway for calcium signaling because we find
copines are capable of interacting with a wide variety o
27 Copines are highly conserved proteins with lipid-binding
28 The biological roles of most
copines are not understood and the biochemical propertie
29 However, the biological functions of the
copines are unknown.
30 A consensus sequence for the coiled-coil
copine-
binding site was derived and found to have predic
31 In the majority of cases the
copine binds to a domain of the target protein that is p
32 The full-length sequences reveal that
copines consist of two C2 domains at the N terminus foll
33 rties and distribution of a native mammalian
copine,
copine I.
34 previously identified several members of the
Copine (
Cpne) family of molecules as potential targets o
35 dicted transmembrane domain and a C-terminal
copine domain and binds to the M-line/dense body protein
36 The
copine domain of RGLG2 exhibited the strongest interacti
37 Antibodies specific to
copine domain protein atypical-1 (CPNA-1), as well as a
38 Copines exhibit a variety of subcellular distributions w
39 ion and immunohistochemistry to characterize
Copine expression in the postnatal and adult mouse retin
40 Thus, the
copine family in Arabidopsis may have effects in promoti
41 gem-4 encodes a member of the
copine family of Ca(2+)-dependent phosphatidylserine bin
42 ding protein of the evolutionarily conserved
Copine family.
43 The
copines,
first described by Creutz et al., comprise a tw
44 The Arabidopsis
copine gene BON1/CPN1 is a negative regulator of cell de
45 The Arabidopsis
copine gene BON1/CPN1 was previously shown to negatively
46 It belongs to the
copine gene family, which is conserved from protozoa to
47 mutant combinations we show that these three
copine genes have overlapping functions essential for th
48 as in other organisms, there is a family of
copine genes, BON1, 2 and 3.
49 mecium was found to have two closely related
copine genes, CPN1 and CPN2.
50 The major protein obtained, named
copine,
had a mass of 55 kDa, bound phosphatidylserine b
51 data bases indicate the presence of multiple
copine homologs in green plants, nematodes, and humans.
52 ally competed by Ca(2+), suggesting that the
copine I A domain may be a functional MIDAS metal bindin
53 Copine I exhibits Mn(2+) binding activity that is strong
54 antiserum raised against a fragment of human
copine I was used to identify chromobindin 17, a secreto
55 A human homolog,
copine I, was expressed in bacteria as a fusion protein
56 d distribution of a native mammalian copine,
copine I.
57 Copine-
I abolishes NF-kappaB transcription by inducing e
58 Knockdown of
copine-
I by siRNA increases tumor necrosis factor alpha-
59 ha-stimulated NF-kappaB transcription, while
copine-
I expression blocks endogenous transcription.
60 Our work provides evidence that
copine-
I regulates the half-life of NF-kappaB transcript
61 Copine-
I stimulates endoproteolysis of p65 within a cons
62 In this study we identify
copine-
I, a calcium phospholipid-binding protein, as a n
63 had correspondingly high kinase activity in
copine III antiserum immunoprecipitate.
64 ified endogenous copine III, and recombinant
copine III expressed in Saccharomyces cerevisiae.
65 ted in all in vitro kinase assays containing
copine III immunoprecipitate or purified copine III.
66 Therefore, the data suggest
copine III may possess an intrinsic kinase activity and
67 no acid analysis revealed phosphorylation of
copine III on serine and threonine residues.
68 Cell lines expressing high levels of
copine III protein had correspondingly high kinase activ
69 The 5 kb
copine III transcript is expressed ubiquitously as deter
70 pine III, chromatography-purified endogenous
copine III, and recombinant copine III expressed in Sacc
71 performed with immunoprecipitated endogenous
copine III, chromatography-purified endogenous copine II
72 ing copine III immunoprecipitate or purified
copine III.
73 fy, partially microsequence, and clone human
copine III.
74 Roles for
copine in binding membranes and target proteins or small
75 Purified native
copine is a 58 kDa monomer that exhibits Ca(2+) self-ass
76 f a 920-base pair partial cDNA revealed that
copine is a novel protein that contains a C2 domain like
77 We also show that interaction with
copines may result in recruitment of target proteins to
78 We provide evidence that
copines,
members of a ubiquitous family of calcium-depen
79 Our results suggest that
Copines might be involved in a combinatorial fashion in
80 Here, we describe a humidity-sensitive
copine mutant in Arabidopsis.
81 The
copine protein is composed of two C2 domains (C2A and C2
82 lysis reveals an unanticipated regulation of
copine protein localization and function by calcium and
83 Copine proteins are highly conserved and ubiquitously fo
84 a way to study steroid hormone signaling and
copine proteins of eukaryotes in a broader perspective.
85 estigations into the important role that the
copine proteins play in vivo.
86 oteins, which are plasma membrane-associated
copine proteins, are critical components of BR signaling
87 ight into the lipid-binding mechanism of the
copine proteins.
88 The
copine target proteins were identified by yeast two-hybr
89 to have predictive value for identifying new
copine targets.
90 A protocol for purifying
copine to homogeneity from bovine spleen is described.
91 ory vesicles, as well the general ability of
copines to bind phospholipid bilayers in a calcium-depen
92 f a CRH-1/CREB transcriptional target (gem-4
Copine),
which parallels the effects of human Shank copy
93 No enzymatic function has been attributed to
copines yet.