ライフサイエンスコーパス: counter selection

1 ences to be removed from the library through counter-selection.

2 are isolated directly using sucrose-mediated counter-selection.

3 ptake patterns, except those related to host counter-selection.

4 Through puromycin selection and ganciclovir counter-selection, a targeting efficiency of over 50% wa

5 mut expression is maintained in vivo with no counter selection against reporter-labeled cells.

6 Twelve rounds of SELEX, including two counter selections against fibroblast cells, were comple

7 ntigen-binding site, with particularly heavy counter-selection against certain productive VH/JH combi

8 Counter-selection against flagella expression was observ

9 unds of cell-SELEX with multiple subtractive counter-selections against non-target species.

10 B is orders of magnitude more sensitive as a counter-selection agent than either gene alone.

11 We conclude that a delicate balance between counter-selection and phage-attack can result in both th

12 rove gene targeting in C. elegans, we used a counter-selection approach to reduce the number of false

13 requency by 1.3E6-fold in total, making tolC counter-selection as effective as most selectable marker

14 cular, it resulted in more B cells surviving counter-selection at the transitional stages.

15 h a polymorphism might be maintained despite counter-selection by malaria, we have carried out functi

16 We fundamentally improved the tolC counter-selection by supplementing a second agent, vanco

17 ulted in many rearranged clones in which the counter-selection cassette had been deleted.

18 ng a second agent, vancomycin, which reduces counter-selection escape by 425-fold, compared colicin E

19 a mismatch repair proficient strain reduced counter-selection escape frequency by 1.3E6-fold in tota

20 ight and guided strain engineering to reduce counter-selection escape frequency by approximately 40-f

21 a selectable/counter-selectable marker, but counter-selection escape frequency using colicin E1 prec

22 encing of 96 independent lineages exhibiting counter-selection escape to identify loss-of-function mu

23 efits to more-cooperative individuals, which counter selection for cheating; however, empirical evide

24 costs of virulence to be the main mechanism countering selection for pathogen complexity, many other

25 nvaluable for genetic manipulation, although counter-selection has historically exhibited limited rob

26 volving the URA3/5-fluoroorotic acid (5-FOA) counter-selection have shown a high background of 5-FOA

27 1 generations, thereby eliminating potential counter selection in females.

28 , and suggest a rationale for their apparent counter selection in RNA processing sites.

29 benefiting one sex may not accumulate due to counter selection in the other sex.

30 uclease-deficient Cas9 to establish a CRISPR counter-selection interruption circuit (CCIC) that can b

31 The two-step process of selection and counter-selection is a standard way to enable genetic mo

32 last selection rounds from both positive and counter selection magnetic beads, several sequences were

33 as a positive-selection marker and GFP as a counter-selection marker which is lost during homologous

34 Additionally, there are few well developed counter-selection markers for use in Burkholderia.

35 ome the limited availability of heterologous counter-selection markers, here we explore novel DNA int

36 The use of SacB as a counter-selection method has been of limited success due

37 In this work, we describe a directed counter-selection method that enables identification of

38 such as BCL-XL is thought to result from the counter selection of sensitive, low expresser clones dur

39 ulated target gene expression and led to the counter-selection of cells containing corrected alleles,

40 in particular in B cells, and how UPR-driven counter-selection of cells undergoing homeostatic failur

41 ction of single-crossover clones followed by counter-selection of double-crossover clones.

42 mplications in both repertoire selection and counter-selection of premalignant clones for leukemia su

43 stance, while plasmid excision is secured by counter-selection of the pIJ2581 glkA gene, which confer

44 Our results indicate that growth reducing counter-selection on a single PV locus results in the st

45 Selection/counter-selection on transfected whole cells yielded hCD

46 Naturally selective forces may create countering selection pressures.

47 recombineering coupled to a robust two-step counter-selection protocol.

48 We are applying counter-selection recombineering to modify bacmid bMON14

49 here should prove useful to others applying counter-selection recombineering to modify BACs or PACs

50 seamless modifications can be achieved using counter-selection strategies in which a donor cassette c

51 We have used a counter-selection strategy based on aberrant phytochrome

52 ed in a DNA cassette and confer a novel dual counter-selection system.

53 me that cannot be directly detected by other counter-selection systems.

54 rcumvents the need for selectable markers or counter-selection systems.

55 on in all isolates, despite the absence of a counter-selection to remove FAD-binding RNAs.

56 rm stable and continuous rounds of selection/counter-selection using tolC, enabling replacement of 10

57 First, a URA3 counter selection was adapted to link chemical dimerizer

58 A genetic system based on rpsL-streptomycin counter selection was developed to further dissect the m

59 Next, the URA3 counter selection was shown to detect cellulase activity

60 of 1264 and BEAS cells used for panning and counter-selection was estimated as 75,000 +/- 5,000 and

61 ic reconstruction, biophysical modelling and counter-selection, we found that tetraploidy evolved bec

62 By performing multiple rounds of counter selections with WT and catalytic dead FphB, we w