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1  does not proceed, possibly because of rapid counterselection.
2 n H. pylori strain 26695 using sacB-mediated counterselection.
3 s associated with the utilization of sacB in counterselection.
4 ing wild-type rpsL and used streptomycin for counterselection.
5 wanted DNA sequences was promoted by sucrose counterselection.
6 e genome editing without base replacement or counterselection.
7 parallel while also eliminating the need for counterselection.
8 tween short-term fitness gains and long-term counterselection.
9 ions in multiple phages without the need for counterselection.
10 oved after isolation of recombinants through counterselection.
11 olecules in a single experiment, without any counterselection.
12  Ig alpha-Ig beta, whereas the mechanism for counterselection against D mu has not been determined.
13            Thus, the molecular mechanism for counterselection against D mu in pre-B cells resembles p
14 f the Ig alpha-Ig beta signal transducers in counterselection against D mu using mice that lack Ig be
15                                              Counterselection against FcgammaRIIB eliminated binders
16                                         With counterselection against non-engineered cells, eight AEG
17 chanism has been proposed to explain TRAPPII counterselection against Rab1.
18         The employment of highly restrictive counterselection and ocr-assisted lambdaRed recombineeri
19 rrelates with the degree of self-reactivity, counterselection, and anergy among individual B cell clo
20 inant effect on altering autoreactive B cell counterselection before any onset of autoimmunity.
21 tial role in controlling autoreactive B cell counterselection by regulating BCR and TLR functions.
22 rategy employing "base-paired" target analog counterselection enhanced specificity by deenriching non
23                                 As a result, counterselection experiments require trial-and-error app
24 strate was used simultaneously with multiple counterselection fluorescent substrates to isolate rare
25 ons, which can be combined with simultaneous counterselection for >99% efficiency.
26 ogous recombination paired with Cas13a-based counterselection for precise phage modifications.
27 variants are susceptible to loss by drift or counterselection imposed by prevailing female preference
28 ions on a single lambda phage genome without counterselection in only a few hours of hands-on time an
29 tasis and suggests that loss of Tfh-mediated counterselection in the GC contributes to lethality in G
30  gene of Escherichia coli functioned for the counterselection in the gonococcus, albeit with low effi
31 tion also affected the efficiency of sucrose counterselection in the sucrose-sensitive strains.
32      Reconstruction experiments suggest this counterselection increased cloning specificity by 100-fo
33 This approach relies on the replacement of a counterselection marker with cargo DNA cassettes via lam
34  propose that scheduled apoptosis provides a counterselection mechanism that keeps the germline stabl
35 ells by Ig membrane mu H chains (mum HC) and counterselection mediated by the truncated HC Dmu depend
36 ted using an allelic replacement and sucrose counterselection method.
37 ting the threshold for B-cell activation and counterselection of autoreactive B cells, making Btk an
38           SAP expression is required for the counterselection of developing autoreactive B cells and
39                           TAK1 also promotes counterselection of NF-kappaB-addicted DLBCL lines by a
40 e observed a pervasive pattern of systematic counterselection of nonsynonymous mutations in prophage
41  two additional rounds of transformation and counterselection of the transformation marker.
42 obacterium that relies on both selection and counterselection of ura3 using a uracil dropout medium a
43  exposed to the stress of transformation and counterselection on 5-fluoro-orotic acid.
44 ive mesothelioma cells and tissues following counterselection on normal cells and identified a panel
45                                        After counterselection, only the desired allele is retained as
46 g phage genomes require laborious screening, counterselection or in vitro construction of modified ge
47 diates effectively determine whether similar counterselection PAMs elicit different selection stringe
48  it is not possible to determine the optimal counterselection parameters.
49 ng plasmid was subsequently cured by sucrose counterselection, resulting in an unmarked P. aeruginosa
50                               This selection-counterselection scheme had acceptable statistics, notwi
51                             We used in vitro counterselection/selection to isolate a pool of RNAs tha
52                                     The rpsL counterselection strategy allows construction of unmarke
53                                We employed a counterselection strategy involving a genetic circuit ba
54 ted by homologous recombination using a sacB counterselection strategy.
55 ost spontaneous mutants, and thus provides a counterselection stringency close to the maximum possibl
56 dhesion receptor subunit Aga2, selection and counterselection substrate sequences, multiple interveni
57 ost aptamer selection efforts have relied on counterselection to eliminate aptamers that exhibit unwa
58  strain was first isolated, followed by sacB counterselection to isolate the double-crossover strain.
59 ced acceptance of small-wing males, imposing counterselection via prevailing preferences.
60                        A cycloheximide-based counterselection was introduced to increase the specific
61                                Cycloheximide counterselection was used in conjunction with 80-bp link
62             To improve the efficiency of the counterselection, we cloned the gonococcal rpsL gene and
63                                      A third counterselection with 5-fluoroorotic acid (5FOA) against
64 gration event can be followed immediately by counterselection, without the need for growth in permiss
65 describe a high throughput screen based on a counterselection yeast two-hybrid assay, which was used