ライフサイエンスコーパス: coverslip

1 olecules located where the cell contacts the coverslip.

2 roelectrodes were microfabricated on a glass coverslip.

3 ivated myosins attach filaments to the glass coverslip.

4 apped and levitated above the surface of the coverslip.

5 tically trapped in solution and adhered to a coverslip.

6 ed to nitrocellulose-coated glass beads on a coverslip.

7 avoiding fluorescence interference from the coverslip.

8 of inner from outer retinal cells across the coverslip.

9 er or exhibited serpentine gliding along the coverslip.

10 the contact area formed by the bead and the coverslip.

11 arose bead resting on a functionalized glass coverslip.

12 ar or parallel to the plane of the substrate coverslip.

13 y was dependent on the myosin density on the coverslip.

14 ection of flow during myosin adhesion to the coverslip.

15 de from titanium and incorporates a standard coverslip.

16 hrough the surface of a rice leaf or plastic coverslip.

17 d pedestals and thus well separated from the coverslip.

18 p and a pedestal bead immobilized on a glass coverslip.

19 e mainly oriented in a flat alignment on the coverslip.

20 resolution over a range of 800 nm above the coverslip.

21 erned atomic-force microscope cantilever and coverslip.

22 ng to the surface of a biotin-modified glass coverslip.

23 res germinating when adhered on a microscope coverslip.

24 med between a plano-convex lens and a planar coverslip.

25 and reperfusion was mimicked by removing the coverslip.

26 oresist on a chemically functionalized glass coverslip.

27 ate by freeing them from the confines of the coverslip.

28 omparison with photobleaching on an uncoated coverslip.

29 icile with the addition of human MUC2-coated coverslips.

30 ter 45 min of acetone fixation of shell vial coverslips.

31 ring fibroblast spreading on collagen-coated coverslips.

32 rabbit retina grown on laminin-coated glass coverslips.

33 -37 were dissected out and cultured on glass coverslips.

34 in unpolarized osteoclasts cultured on glass coverslips.

35 ns: microfluidic devices and chitosan-coated coverslips.

36 d supported membranes on silica beads and on coverslips.

37 y available microprisms attached to standard coverslips.

38 samples are prepared conventionally on glass coverslips.

39 it flat lamellipodia on poly-L-lysine-coated coverslips.

40 20 arrays of gold nanostructures atop glass coverslips.

41 20-fold more slowly than the sample on glass coverslips.

42 rs tethered to the surface of functionalized coverslips.

43 sustained, compared to sperm with unmodified coverslips (12.0 vs. 1.0%, p < 0.05).

44 f a convex lens is used to deform a flexible coverslip above a substrate containing embedded nanotopo

45 ound the ultrafast TCA reading of GNS-coated coverslips achieved a maximal signal amplification (57-f

46 ing blebbistatin to the cells or use of soft coverslips, also formed microtubule-dependent dendritic

47 footprint (surface area in contact with the coverslip), an effect previously attributed to exocytic

48 Approximately 25,000 RGCs were seeded per coverslip and cultured for 3 days before testing.

49 n allow a particle to be moved away from the coverslip and into solution, avoiding fluorescence inter

50 lecule localization microscopy away from the coverslip and into thicker specimens will greatly broade

51 s from steric hindrances from the microscope coverslip and other surface effects.

52 tro when plated onto a localized region of a coverslip and overlaid with neonatal rat ventricular myo

53 ancement of tight junction formation on both coverslip and permeable inserts, in addition to enhancin

54 an array within a thin film of aluminum on a coverslip and planarized to achieve no height distinctio

55 events at the contact point between a glass coverslip and the plasma membrane.

56 formation at the interface between the glass coverslip and the polymer micropallet.

57 ess when the distance between the microscope coverslip and the trap focus is being changed.

58 s and fixed before centrifugation onto glass coverslips and air drying.

59 operties to a variety of substrates, such as coverslips and Eppendorf tubes.

60 minate combustible materials such as plastic coverslips and grease.

61 Fixed yeast cells are attached to coverslips and hybridized with a mixture of FISH probes,

62 ith a mixture of smFISH probes, mounted onto coverslips and imaged in 3D.

63 vine serum albumin and were dried onto glass coverslips and kept at different relative humidities.

64 in intracellular pH (pHi) in cells grown on coverslips and loaded with 2',7'-bis(2-carboxyethyl)-5(6

65 Mitochondria were attached to coverslips and perfused with K+-based buffer containing

66 m marked the location of cultured neurons on coverslips and provided unequivocal relocation of ATP-re

67 Mitochondria were attached to coverslips and superfused with K(+)-based HEPES-buffer m

68 Chinese hamster ovary cells were adhered to coverslips and then allowed to bind labeled microtubules

69 BCECs were grown on glass coverslips and then perfused with HCO(3)(-)-free Ringer'

70 resence of an effectively infinite wall, the coverslip, and of the immobile pedestal.

71 he central portion of monolayer with a glass coverslip, and reperfusion was mimicked by removing the

72 cles that are attached to the surface of the coverslip, and the sensitivity and selectivity of detect

73 to colonize and form biofilms on MUC2-coated coverslips, and 16S rRNA sequencing showed a unique biof

74 , cells were passaged onto rectangular glass coverslips, and anisotonically induced volume changes we

75 cells were isolated from rat lens, plated on coverslips, and placed in a temperature-controlled chamb

76 lls tethered by a single flagellum to quartz coverslips; and 3). the motor response time of individua

77 rd modifications of the typical slide-sample-coverslip approach to microbial imaging-that controls te

78 croscopy (OSAM) technique which can search a coverslip area of 50 x 24 mm(2) in just 5.3 min and loca

79 eeks in advance, and it takes 5 d to prepare coverslips as a substrate for neuronal growth.

80 hoeae forms a biofilm in flow cells on glass coverslips as well as on primary cervical epithelial cel

81 lens fiber cells were isolated and plated on coverslips at the bottom of a temperature-controlled cha

82 heavy chain attached to streptavidin-coated coverslips at various surface densities.

83 Coverslip-attached cells were perfused in an imaging cha

84 Individual coverslip-attached fibroblasts were loaded with the calc

85 stomer, polydimethylsiloxane on a microscope coverslip base.

86 t" approach, immobilizing viral particles on coverslips before seeding live MHCII-expressing cells on

87 to a fluid inlet, a circular portion of the coverslip beneath the well exploded outward but very lit

88 ity are largely unaffected by contact to the coverslip but the measured localization densities are in

89 chia coli was tethered covalently to a glass coverslip by a single flagellum, and its rotation was st

90 of Escherichia coli were tethered to a glass coverslip by means of a single flagellum: such cells rot

91 bacterium Escherichia coli tethered to glass coverslips by single flagella.

92 abilized grids or poly-L-lysine-coated glass coverslips by transmission electron microscopy or immuno

93 was routinely cultured and seeded onto glass coverslips (c-met immunodetection), 12-well plates (DNA

94 nonexponential distribution, confirming that coverslips can affect microtubule dynamics.

95 ers and focal adhesions) typical of cells on coverslips, cells spread with lamellipodial extensions a

96 it from the close approximation of slide and coverslip ("chimneying").

97 cle is fluorescently labeled and placed on a coverslip coated with a thin layer of noble metal.

98 g of a nonadhesive agar (agarose) onto glass coverslips coated with a cell adhesive film (poly(L-lysi

99 s of activated T cells, we plated T cells on coverslips coated with antibodies to the T cell receptor

100 npermissive clone 17 (minus) was adherent to coverslips coated with chemokines, was phagocytic, kille

101 e objective (1.65) and high-refractive-index coverslips coated with gold, it is possible to follow ro

102 ed blood eosinophils were incubated on glass coverslips coated with intravenous immunoglobulin and in

103 cytes plated on uncoated coverslips (LMN) or coverslips coated with LMN (+LMN).

104 damage can be achieved by imaging samples on coverslips coated with monolayers of silver nanoparticle

105 and protein L-antibody interactions on glass coverslips coated with polyethylene glycol.

106 molecules of actin of a myofibril on Olympus coverslips coated with SIF decreased at least 30-fold in

107 lightly with rhodamine-phalloidin, placed on coverslips coated with SIF, illuminated by total interna

108 easured fluorescence from a sample placed on coverslips coated with silver island films (SIF).

109 Within seconds of contacting coverslips coated with stimulatory antibodies, T cells d

110 HLE on the cell surface and was adherent to coverslips coated with the HLECS ligands alpha1proteinas

111 titate CLAN formation in HTM cells plated on coverslips coated with various extracellular matrix (ECM

112 Using a micropatterned hydrogel-laden coverslip compatible with standard fluorescence microsco

113 cked by areas where the MT was anchored to a coverslip, consistent with a coupling structure encircli

114 by hrpA mutants for 48 h on collagen-coated coverslips demonstrated significant reductions compared

115 acilitates DNA loading, stretching and glass coverslip deposition from microliter-scale DNA samples.

116 Cells were grown at low density on glass coverslips, encased in a flow-through chamber, and conti

117 lity assays, co-sedimentation assays, and on-coverslip enzyme-linked immunosorbent assays to quantify

118 in RPMI 1640 with 10% fetal bovine serum on coverslips for 8 days or assayed for colony-forming effi

119 ree medium for approximately 2 days on glass coverslips for subsequent patch-clamp studies employing

120 d preparation of nanofibers aligned on glass coverslips for the study of membrane wrapping by rodent

121 dipocytes, grown and differentiated on glass coverslips, for at least 2 h.

122 dendritic extensions, whereas fibroblasts on coverslips formed lamellipodial extensions and were spre

123 flux from HRP-catalyzed assays on disposable coverslips functionalized with HRP and placed proximal t

124 Glass coverslips gave no response under all experimental condi

125 In cells grown on glass coverslips, GFP-CFTR was polarized to the basolateral me

126 er dye washout and tested our method on both coverslip-grown cell clusters and filter-grown intact mo

127 stiff (10 kPa) gels or collagen-coated glass coverslips, however, had broad morphologies, formed abun

128 ent support substrates: (i) microscope slide coverslips; (ii) Al foil; and (iii) Cu tapes.

129 cue positions of microtubules nucleated from coverslip-immobilized seeds displayed a nonexponential d

130 ot' for performing circular craniotomies for coverslip implantation, large craniotomies for implantin

131 of Listeria moving between a glass-slide and coverslip in a Xenopus frog egg extract motility assay.

132 d from rat liver or from cultured cells on a coverslip in the form of a proteinaceous planar membrane

133 ts and cultured on polylysine/laminin-coated coverslips in serum-free medium for 2 days.

134 from blood samples and allowed to spread on coverslips in the presence or absence of LPS purified fr

135 film formation on human gallstones and glass coverslips in vitro, including confocal, light, and scan

136 ime to virtually any cell type attached to a coverslip, independently of cellular shape and geometry.

137 n cardiac monolayers, upon reperfusion after coverslip-induced ischemia, mitochondrial inner membrane

138 e modelled as in vitro "microcosms" on glass coverslips inoculated with the natural microbial populat

139 in volumes comparable to those used by glass coverslips is presented.

140 and well-spread primary islet cells on glass coverslips is required for detailed imaging studies by s

141 During coverslip ischemia, action potential duration shortened,

142 Because the PDMS chambers are bonded to a coverslip, it is possible to image P. patens development

143 isolated atrial myocytes plated on uncoated coverslips (LMN) or coverslips coated with LMN (+LMN).

144 Calu-3 cells were grown on coverslips, loaded with the pH-sensitive fluorescent dye

145 pattern totally internally reflected at the coverslip-medium interface which excites fluorescence on

146 lyspermic zygotes was reduced in the suLe(X)-coverslip method (17.7 vs. 41.3%, p < 0.05).

147 HepG2 cells were cultured in pairs on glass coverslips micropatterned with polyethylene glycol disil

148 r observed for enterocyte migration on glass coverslips, namely the dependence of migration speed on

149 Coverslips of cultured human coronary artery ECs were pl

150 ished as monolayers on glass collagen-coated coverslips or on semipermeable membranes at an air-fluid

151 old and tenfold, respectively, compared with coverslips or rigid PMAs.

152 ution by distributing labeled genomic DNA on coverslips or slides for immunodetection.

153 ion depth, by a fluorescence microscope on a coverslip, or uptaken in a single HeLa cell.

154 wild-type and fap1 mutant strains on plastic coverslips over time.

155 Compared with cells on collagen-coated coverslips, phagocytosis of fibronectin-coated beads by

156 her throughput multi-well imaging via remote coverslip placement.

157 owth factor (VEGF-165), integrate with glass coverslips, polyacrylamide gels, and collagen scaffolds,

158 conducted to identify phenotypes followed by coverslip removal and hematoxylin and eosin staining of

159 ile the physical sweeping step using a glass coverslip removes the excess cells outside the microwell

160 ques, and surface blotting of plaques onto a coverslip, revealed that fluorescent puncta were probabl

161 f adherent M0 cells grown on chambered glass coverslips, revealed agreeing platinum concentrations at

162 cells plated on extracellular matrix-coated coverslips showed enhanced invadopodia formation in resp

163 SLG adsorbed to a glass microscope coverslip (SLG/SiO(2)) served as a platform for the esti

164 ol should take 1 h 30 min including time for coverslipping slides.

165 5 mM were observed in neurones on 27% of the coverslips studied.

166 ion fold, we also explored transparent glass coverslip substrate as a model microfluidic immunoassay

167 Consequently, compared with a glass coverslip substrate, the presented SPCE substrate demons

168 d, and emission coupling yield through glass coverslip substrates coated with metal layers of differe

169 7 nm Au nanoparticles immobilized on a glass coverslip surface is analyzed by a Matlab code before an

170 ing and astrocytes or BON cells grown on the coverslip surface modify the dark-field signal.

171 n optically trapped bead in contact with the coverslip surface while observing the bead's thermal mot

172 tethering either DNA or PcrA to a microscope coverslip surface, unwinding of both linear and natural

173 , AtKCBP no longer binds microtubules to the coverslip surface.

174 ly) at depths approximately 250 mum from the coverslip surface.

175 ergistic combination of vacuum degassing and coverslip sweeping.

176 In a myocyte lying flat on a coverslip, t-tubules typically progressed from its upper

177 Fibroblasts on collagen-coated coverslips that were unable to develop stress fibers and

178 rface membrane in close contact with a glass coverslip (the "footprint").

179 For vesicles adhered to a coverslip, the confocal probe can be scanned through the

180 ntation of a headframe and a removable glass coverslip, the precise positioning of the Neuropixels pr

181 Immobilized on glass coverslips, the thin-film MIPs of 1 displayed a clear si

182 Finally, the ability of the suLe(X)-coverslip to maintain sperm fertilizing ability over tim

183 croscopy from selected cellular targets near coverslips to intra- and extracellular targets deep insi

184 Dinarina et al. grow "fields" of spindles on coverslips to investigate the relationship between chrom

185 ft lithography, and of silane-modified glass coverslips to reversibly seal the open side of the chann

186 ting of a glass surface (either a slide or a coverslip) to which an oviduct glycan (sulfated Lewis X

187 hering to flat surfaces such as a microscope coverslip transmit cytoskeletally generated forces to th

188 Specimens are supported on CaF(2) coverslips under ambient conditions from where we (1) qu

189 ficiently on MAdCAM-1- than on VCAM-1-coated coverslips under conditions of flow.

190 r pH was monitored by imaging BCECF fixed to coverslips under dissociated cells.

191 erical ghosts were centrifuged onto a coated coverslip upon which they adhered.

192 ovalently attached to the surface of a glass coverslip using a fixed ratio of a native nucleotide and

193 es adjustment of bead height relative to the coverslip using a variant of optical force microscopy (t

194 erated by tethering growing plus-ends to the coverslip using lambda-DNA, led to an approximately seve

195 The suLe(X)-coverslip was adapted for in vitro fertilization (IVF),

196 The coverslip was mounted on a computer-controlled piezo-ele

197 fertilization by sperm bound to the suLe(X)-coverslip was sustained, compared to sperm with unmodifi

198 ches of the LIS of MDCK cells grown on glass coverslips was indistinguishable from that in free solut

199 ng the attachment of the axonal shaft to the coverslip, we estimate values for the axial viscosity of

200 s to quantify proteins on the motility assay coverslip, we provide strong evidence that most of the M

201 By imaging samples on reflective coverslips, we enable simultaneous collection of four co

202 Additionally, using the chitosan-coated coverslips, we show that real-time environmental changes

203 Cells on companion coverslips were fixed with 3% p-formaldehyde and permeab

204 cropores in 130 mum thick borosilicate glass coverslips were functionalized with trichloro(1H,1H,2H,2

205 Monolayers grown on glass coverslips were loaded with the pH-sensitive dye 2', 7'-

206 MK adhering to FN or FGN coated on coverslips were quantitated by a computerized image anal

207 reshly secreted plaques deposited onto glass coverslips were shown by matrix-assisted laser desorptio

208 Cells grown on coverslips were similarly treated and probed with antibo

209 The coverslips were then moved to dishes containing either c

210 Cells washed from coverslips were used to determined viable counts.

211 Our array-based technology enables "active coverslips" where, when scaled, many individually held c

212 ack-scattered light between the bead and the coverslip, whereas the noise inherent in a surface-tethe

213 e neurons are cultured on polylysine-treated coverslips, which are suspended above an astrocyte feede

214 and was chemisorbed to a standard microscope coverslip with (3-mercaptopropyl)trimethoxysilane.

215 single NP-UCNP in solution between slide and coverslip with diffusivity up to 10(-2) mum(2)s(-1).

216 Cells were grown on glass coverslips with a microgrid pattern so that the results

217 Coverslips with cultured human coronary artery ECs were

218 Resting MK adhered to coverslips with immobilized FN.

219 s were cultured overnight on collagen-coated coverslips with increasing concentrations of different b

220 rse cultures of hippocampal neurons grown on coverslips with no glia.

221 rulean-labeled protein proximal to the glass coverslip within individual granules.

222 Cells were attached to glass coverslips within flow chambers that allowed application

223 g dilute solutions of Sylgard 184 onto glass coverslips, yielding approximately 1.4 microm thick film

224 ndrical shapes and so lie flat on microscope coverslips, yielding low-resolution images when observin