ライフサイエンスコーパス: cross-hybridization

1 ization specificity and minimize genome-wide cross-hybridization.

2 nd may lead to false positive results due to cross-hybridization.

3 composition and those likely resulting from cross-hybridization.

4 specific hybridization and oligo genome-wide cross-hybridization.

5 tion and dramatically decreasing genome-wide cross-hybridization.

6 tive template secondary structure to control cross-hybridization.

7 ion levels are more complex, probably due to cross-hybridization.

8 ransposon-disrupted gene exhibit significant cross-hybridization.

9 amilies of closely related genes may lead to cross-hybridization.

10 75% similar over the 50 base target may show cross-hybridization.

11 ligonucleotide; and there was no evidence of cross-hybridization.

12 erature, and we validated for the absence of cross-hybridization.

13 es, as well as by interspecies, whole-contig cross-hybridizations.

14 ology is isoform-specific, with virtually no cross-hybridization, achieving limits of detection (LODs

15 probe design rules that significantly reduce cross-hybridization after their introduction into the fr

16 ization signals are derived from genome-wide cross-hybridization alone.

17 t the criterion of 90% identity suggests how cross hybridization among gene family members can overes

18 It may be a consequence of wide-cross hybridization and may contribute to quantitative t

19 Based on cross hybridization and sequence comparisons, homologous

20 The two probes show no cross-hybridization and are both species specific for C.

21 isclassifications in replicate measurements, cross-hybridization and sensitivity limitations.

22 fic to their respective targets to avoid any cross-hybridization and should not form stable secondary

23 ksA, was cloned from Aspergillus nidulans by cross-hybridization, and the corresponding protein was p

24 epared and analyzed, melting transitions for cross-hybridization are observed along with significant

25 ology, without labeling and without apparent cross-hybridization artifacts, would allow fast, sensiti

26 otide annealing to non-target sites, termed 'cross-hybridization', as a significant contributor to mu

27 sequence-specific allelic imbalances such as cross-hybridization between allele A and allele B probes

28 targets were orthogonally designed to avoid cross-hybridization between capture probes.

29 labeled P450 genes exhibited essentially no cross-hybridization between families and within subfamil

30 ligned contigs of 399 cosmids established by cross-hybridization between the cosmids, which were sele

31 ted systematic base-calling errors caused by cross-hybridization between the whole-genome sample and

32 tion, we developed a strategy to correct for cross-hybridization biases of gene-level expression esti

33 al bacteria), despite the high potential for cross-hybridization by hundreds of different coexisting

34 The assay was tested for cross-hybridization by using large numbers (e.g., 10(5)t

35 ngle-copy tile path, as significant sequence cross-hybridization can result from the presence of non-

36 their signals are derived from specific and cross-hybridization components combined together in a to

37 re and GC content, as well as redundancy and cross-hybridization constraints.

38 e hybridization affinities and the degree of cross-hybridization due to indeterminable thermodynamic

39 etween closely related isoforms, e.g. due to cross-hybridization during their detection.

40 Little is known about the details of cross-hybridization effect at molecular level.

41 enous treatment effects and reduces possible cross-hybridization effects across a set of probes.

42 ineage but can be biased by inter-subspecies cross-hybridization events during speciation.

43 metry (ITC), vapor pressure osmometry (VPO), cross-hybridization experiments involving the analysis o

44 r to estimate the extent of non-specific and cross-hybridization for each probe.

45 t where the identification is complicated by cross-hybridization from splice variants or closely rela

46 tween specific hybridization and genome-wide cross-hybridization has been insufficiently studied, des

47 hese estimates are affected by biases due to cross-hybridization, in which probes hybridize to off-ta

48 These 187 clones were then sorted by filter cross-hybridization into 34 unique groups.

49 encing data demonstrates that correction for cross-hybridization leads to a significant improvement o

50 hybridization, implying that the sources of cross-hybridization must be very different between a PM-

51 w signal affected by spurious contributions (cross-hybridization, noise, background, and unequal spec

52 Sequencing and cross-hybridization of amplification products indicated

53 es with a single A/G mismatch are formed via cross-hybridization of mutant (T-->G) and wild-type DNA-

54 Cross-hybridization of phage genes on the array with rel

55 Cross-hybridization of repetitive sequences in genomic a

56 Cross-hybridization of the mouse cDNA to a human thymus

57 Almost all miRNA sensors utilize cross-hybridization of the target miRNA with a capture p

58 acts are more likely to be due to systematic cross-hybridization of these poly(dT) tracts than to tru

59 Here, we report a free energy analysis of cross-hybridization on short oligo microarrays using dat

60 Our analysis revealed that cross-hybridization on the arrays is mostly caused by ol

61 i specificity by reducing potential sequence cross-hybridization or microRNA-like effects.

62 of a universal array with minimal amounts of cross-hybridization or misligation.

63 inherent to short oligonucleotide arrays -- cross-hybridization, or variability in probe response to

64 designed mismatches can be used to decrease cross-hybridization potential, but implementing all poss

65 To help predict potential cross hybridization, PROBEmer computes all near neighbor

66 embled in contigs that were established from cross-hybridization relationships between the cosmids.

67 A novel cross-hybridization score, quickly computable for any pl

68 Such cross-hybridization "side reactions" are of particular n

69 s containing G-stacks tend to have increased cross hybridization signals and reduced target-specific

70 homologies, characterized by the presence of cross-hybridization signals on one or more telomeres in

71 ter subtraction of the artificial, random or cross-hybridization signals, data about 5315 genes have

72 oration of an oligonucleotide at a predicted cross-hybridization site, and by modification of putativ

73 specific parameters (cell line, degradation, cross-hybridization, target conformation, etc.) can be p

74 ated to be energetically much more costly in cross-hybridization than that in gene-specific hybridiza

75 go microarray technology is how to deal with cross-hybridization that produces spurious data.

76 educes the potential for artifacts caused by cross-hybridization, the subsampling of the query genome

77 song exposure and by demonstrating effective cross hybridization to genomic DNAs of other songbird sp

78 Probes for 20 human genes were tested for cross-hybridization to murine BAC and PAC clones, thereb

79 ted by different levels of non-specific- and cross-hybridization to non-specific transcripts.

80 for target genes/genomic sequences, however, cross-hybridization to non-target sequences becomes a pr

81 d Fas were detected in all SCCHN tested, and cross-hybridization to radioactive Fas and FasL cDNA pro

82 Due to the problem of potential cross hybridization, using full-length genes for microar

83 everal issues inherent to these arrays (e.g. cross-hybridization, variable probe/target affinity) cau

84 ed at one to two copies of mRNA per cell and cross hybridization was estimated to occur if closely re

85 Cross-hybridization was confirmed conclusively by partia

86 Analysis demonstrated that cross-hybridization was the chief source of FPs, while T

87 By DNA cross-hybridization we have identified two Meis1-related

88 n match type to a transcript are affected by cross-hybridization, we developed a strategy to correct

89 To study cross-hybridization, we map Affymetrix exon array probes

90 Regions of cross-hybridization were co-linear between the two genom

91 verage signal intensity, and an estimator of cross-hybridization were found to be associated with the

92 None of the OsA-CL cDNA clones showed cross hybridization with the TX3868 cDNAs suggesting tha

93 By cross-hybridization with DcR1, we have identified a four

94 assay for a number of samples may be due to cross-hybridization with HPV types not included in the H

95 to known disease resistance genes, showed no cross-hybridization with maize genomic DNA, suggesting s

96 -established hybridization model to estimate cross-hybridization with nontarget sequences, show that

97 esult in robust and consistent evaluation of cross-hybridization with nontarget sequences.

98 idization affinities should be enhanced, and cross-hybridization with off-targets must be minimized.

99 inone redox signal as measured by SWV and no cross-hybridization with single nucleotide mismatch DNA

100 o the concerns for enzymatic degradation and cross-hybridization with the host's genetic materials.

101 or of nonspecific probe-target interactions (cross-hybridization) with an optimization algorithm that